A jellyfish discoid body separation and selection device

Abstract

This utility model discloses a jellyfish disc-shaped body separation and selection device, belonging to the technical field of aquatic organism culture and separation devices. It includes a polyp culture chamber, a disc-shaped body screening pipe, and a circulating filtration device. An inlet pipe is installed inside the polyp culture chamber. The disc-shaped body screening pipe consists of multiple screening units connected in series, with sieves at the connection points. The mesh size of the multiple sieves decreases sequentially along the flow direction. Each screening unit has a sealing cap at its lower part. The circulating filtration device includes a circulating water tank and a variable frequency water pump. The outlet of the polyp culture chamber is connected to the inlet of the disc-shaped body screening pipe, and the outlet of the disc-shaped body screening pipe is connected to the inlet of the circulating filtration device. The outlet of the variable frequency water pump is connected to an outlet pipe, the end of which is connected to the inlet pipe. This utility model can achieve automatic separation and automatic screening, saving manpower and time costs, reducing human damage and stress, and improving the accuracy of the experiment.

Description

Technical Field

[0001] This utility model relates to the technical field of aquatic organism culture and separation devices, and in particular to a jellyfish disc-shaped body separation and selection device. Background Technology

[0002] Moon jellyfish, belonging to the phylum Cnidaria, class Scyphozoa, order Scyphozoa, family Phyllotyzoideae, and genus *Leptochloa*, is a cosmopolitan species widely distributed in coastal waters between 70°N and 40°S. In the past decade or so, large-scale outbreaks of moon jellyfish have occurred worldwide, severely impacting fisheries, industry, and coastal tourism. On the other hand, with ornamental jellyfish becoming a new favorite in the aquarium market, the moon jellyfish, with its graceful and flowing movements in the water, offers excellent visual appeal, making research on moon jellyfish a global research hotspot. The life cycle of the moon jellyfish consists of an asexual polyp stage and a sexual medullary stage. Most current research focuses on the polyp stage, where the polyp releases discoids through transverse splitting; therefore, the polyp population size determines the number of discoids released. However, on the other hand, the benthic, sessile polyps release discoids, thus completing the transition from the asexual to the sexual generation. The survival and growth of the discoids released during the transverse splitting stage also play a crucial role in the size of the medullary population. As early larvae in the planktonic stage, the discoids are more sensitive to changes in environmental physicochemical factors and experimental procedures, and their survival rate during the culture stage is significantly affected by environmental and human factors.

[0003] Current research on discoid polyps typically involves culturing polyps on corrugated plates in jellyfish tanks to obtain a sufficient quantity or to study the number of polyps released. The temperature is controlled to induce transverse splitting of the polyps, releasing the discoid polyps. During the release phase, the polyps are removed daily using pipettes and transferred to a temporary holding tank. At the peak of polyp splitting, thousands of discoid polyps can be released daily, resulting in significant labor costs for separation and selection. Furthermore, the newly released discoid polyps are small, some even adhering to the corrugated plates. Their transparent appearance and small bell diameter further hinder transfer, making it impossible to transfer all. Unsuccessful transfers may lead to loss during subsequent feeding and water changes, affecting the accuracy of data analysis. Additionally, the process of removing and transferring the polyps using pipettes causes mechanical damage and stress to the polyps. On the other hand, the disc-shaped organisms transferred to the disc-shaped organism incubator vary in length and size. In subsequent group experiments, they need to be grouped according to different umbrella diameters. Mixing disc-shaped organisms of different umbrella diameters together and selecting them will consume a lot of manpower and time.

[0004] Therefore, there is an urgent need to develop a new type of disc-shaped body separation and screening device. Utility Model Content

[0005] The main purpose of this invention is to provide a jellyfish disc-shaped body separation and selection device to solve the above-mentioned problems.

[0006] To achieve the above objectives, this utility model provides a jellyfish disc body separation and selection device, including a polyp culture tank, a disc body screening pipe, and a circulating filtration device; the polyp culture tank is equipped with an inlet pipe, and the lower part of the side wall of the polyp culture tank is provided with an outlet; the disc body screening pipe is composed of multiple screening units connected in series, and a sieve is provided at the connection between two adjacent screening units; the mesh size of the multiple sieves decreases sequentially along the flow direction; each screening unit is provided with a sealing cover at its lower part; the circulating filtration device includes a circulating water tank and a variable frequency water pump installed in the circulating water tank, and the lower part of the side wall of the circulating water tank is provided with an inlet; the outlet of the polyp culture tank is connected to the inlet of the disc body screening pipe, and the outlet of the disc body screening pipe is connected to the inlet of the circulating filtration device; the outlet of the variable frequency water pump is connected to an outlet pipe, and the end of the outlet pipe is connected to the inlet pipe of the polyp culture tank.

[0007] Furthermore, the polyp incubator is cylindrical.

[0008] Furthermore, the water inlet pipe is cylindrical and vertically arranged. The top end of the water inlet pipe is located outside the polyp incubator and connected to the water outlet pipe. The bottom end of the water inlet pipe is a closed end and connected to the bottom plate of the polyp incubator. There are multiple small circular holes arranged in a straight line on the side wall of the water inlet pipe.

[0009] Furthermore, the screening unit is cylindrical with sealing flanges installed at both ends, and adjacent screening units are connected by sealing flanges.

[0010] Furthermore, the circulating water tank contains filter material.

[0011] Furthermore, a temperature control device is installed inside the circulating water tank.

[0012] This utility model has the following beneficial effects:

[0013] This invention can automatically separate polyps and discoids and automatically screen them according to their umbrella diameter. It integrates separation and screening functions, saving manpower and time costs. When separating discoids, simply open the sealed cap and directly transfer the water containing the discoids. Compared with the experimenter using a pipette to suck them up, it reduces human damage and stress, and improves the survival rate of discoids and the accuracy of indoor experiments. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of the jellyfish disc-shaped body separation and selection device of this utility model.

[0015] Figure 2 This is a schematic diagram of the screening unit of a jellyfish disc-shaped body separation and selection device according to the present invention.

[0016] Among them, 1-polyp incubator; 2-screening unit; 3-sieve silk; 4-circulating filter device; 11-water inlet pipe; 12-circular small hole; 13-corrugated plate; 21-sealing flange; 22-sealing cover; 41-circulating water tank; 42-variable frequency water pump; 43-water outlet pipe. Detailed Implementation

[0017] To achieve the above objectives and effects, the technical means and structure adopted by this utility model are described in detail with reference to the accompanying drawings, focusing on the features and functions of the preferred embodiments of this utility model.

[0018] like Figure 1-2As shown, this utility model provides a jellyfish disc body separation and selection device, including a polyp culture box 1, a disc body screening pipe, and a circulating filtration device 4. The polyp culture box 1 is cylindrical, reducing the formation of corners inside the box and increasing the smoothness of the disc body's movement. This effectively solves the problem of existing square culture bottles, which produce bottom corners and cause disc bodies to easily get stuck in the corners, resulting in incomplete separation of disc bodies. A water inlet pipe 11 is installed inside the polyp culture box 1. The water inlet pipe 11 is cylindrical and vertically arranged. The top end of the water inlet pipe 11 is located outside the polyp culture box 1 and is connected to the water outlet pipe 43. The lower end of the water inlet pipe 11 is a closed end and is connected to the bottom plate of the polyp culture box 1. The side wall of the water pipe 11 has multiple small circular holes 12 arranged vertically in a straight line. This linearly arranged porous structure effectively generates flowing water at different levels inside the incubator, preventing the released disc-shaped bodies from adhering to the corrugated plate. Since the newly released disc-shaped bodies have weak mobility, a single inlet may cause them to gather in a corner of the incubator, failing to separate successfully. The multi-hole inlet design effectively increases the flow of water within the incubator, increasing the number of disc-shaped bodies separated. The lower side wall of the polyp incubator 1 has an outlet. The disc-shaped body screening pipe consists of multiple screening units 2 connected in series. Each screening unit 2 is cylindrical and has sealing flanges 21 installed at both ends. Adjacent screening units 2... The two screening units 2 are connected by a sealing flange 21; a screen 3 is provided at the connection between two adjacent screening units 2; the mesh size of the screen 3 can be set according to the umbrella diameter of the target disc-shaped body; the mesh size of multiple screens 3 decreases sequentially along the flow direction so as to obtain disc-shaped bodies with different umbrella diameters in each screening unit; each screening unit 2 is provided with a sealing cover 22 at the bottom, and the disc-shaped bodies obtained inside can be separated after the sealing cover 22 is opened; when a certain number of disc-shaped bodies are obtained, the circulating filtration device 4 is paused. Since the newly released disc-shaped bodies have weak movement ability, there will be no disc-shaped body backflow phenomenon, so it is not necessary to disassemble the screening unit 2. The sealing cover 22 at the bottom of each screening unit 2 can be opened directly to transfer the water inside. Move the samples to the corresponding incubators, then disassemble each screening unit 2. Gently rinse the screening unit 2 and the corresponding sieve 3 with water to completely separate the remaining disc-shaped bodies attached to the sieve 3 and the screening unit 2. The circulating filtration device 4 includes a circulating water tank 41 and a variable frequency water pump 42 installed in the circulating water tank 41. The lower part of the side wall of the circulating water tank 41 is provided with a water inlet. The water outlet of the polyp incubator 1 is connected to the water inlet of the disc-shaped body screening pipe, and the water outlet of the disc-shaped body screening pipe is connected to the water inlet of the circulating filtration device 4. The water outlet of the variable frequency water pump 42 is connected to a water outlet pipe 43, and the end of the water outlet pipe 43 is connected to the water inlet pipe 11 of the polyp incubator 1.Adjusting the flow rate of the variable frequency water pump 42 can control the water flow speed within the entire separation and sorting device, preventing damage to the polyps and discs caused by excessive flow. Filter materials, such as coral stone and ceramic rings, can be placed in the circulating water tank 41 to ensure water quality. At the same time, temperature control devices, dissolved oxygen control devices, and other water physicochemical factor controllers can be installed to adjust the water physicochemical factors externally, achieving precise control of the water physicochemical factors without affecting the polyps and discs.

[0019] This utility model also provides a method for using the jellyfish disc-shaped body separation and selection device, including the following steps:

[0020] S1. Trim the corrugated plate 13, which is covered with polyps of moon jellyfish in the laboratory, to a suitable size, record the number of polyps on the corrugated plate 13, and then place it in a polyp incubator.

[0021] S2. Turn on the circulating filter device 4 to allow the water temperature to adapt. During the adaptation phase, the water temperature should be controlled at around 18℃.

[0022] S3. The experiment consisted of 5 temperature change groups. After the polyps adapted for one week, the water temperature was gradually adjusted, decreasing or increasing at a rate of 1℃ per day. After reaching the preset experimental temperature, the polyp transverse cracking test was carried out.

[0023] S4. In the early stage of polyp transverse splitting, closely monitor whether discoids are released in polyp incubator 1. When the polyps enter the transverse splitting stage, adjust the water flow rate to achieve water flow without affecting the individual movement posture of the discoids.

[0024] S5. Each temperature change group has 5 umbrella diameter ranges. Therefore, the disc-shaped body separation pipeline has a total of 5 screening units 2.

[0025] S6. During the polyp transverse splitting stage, at a fixed time each day, pause the circulating filtration device 4, open the sealing cover 22 at the bottom of the 5 screening units, transfer the water to the corresponding incubator, then disassemble the screening unit 2, rinse the screening unit 2 and the corresponding sieve 3, so as to separate all the remaining unseparated discoids, and record the number of discoids of 5 sizes in 5 temperature change groups.

[0026] S7. Install screening unit 2 and repeat this step daily during the polyp transverse splitting stage, recording the number of discs placed.

[0027] S8. Culture discoids of different release times and sizes separately to continue early discoid experiments. The experiment is set up with 5 body length groups. At this time, healthy individuals can be randomly selected from incubators of different body lengths without measuring the umbrella diameter, making the experiment more convenient and accurate.

[0028] This separation and selection device can achieve precise control of the physicochemical factors of the water, ensuring the consistency of water indicators throughout the entire separation device. Especially for sensitive physicochemical factors such as dissolved oxygen, simply adjusting the water in the circulating water tank 41 to the required standard creates an internal circulation within the entire separation and selection device, guaranteeing precise control of physicochemical factors. Moreover, the circulating filtration device 4 exists independently outside the polyp incubator 1 and the disc separation pipeline, making the adjustment of physicochemical factors more convenient. The adjustment process will not affect the polyps and discs within the device. When separating discs, simply open the sealing cap 22 to directly transfer the water containing the discs. Compared to using a pipette to extract the discs, this reduces human damage and stress, lowers labor costs, and increases the accuracy of indoor experiments.

[0029] In addition to separating and selecting the disc-shaped bodies of moon jellyfish, this invention can also be used to conduct experiments on the disc-shaped bodies of different jellyfish species. Furthermore, the size of the screening unit 2 can be adjusted according to the size of different test organisms, making it applicable to a wider range of biological species.

[0030] The above description is only a preferred embodiment of the present utility model and not all embodiments. Anyone should know that structural changes made under the guidance of the present utility model are protected by the present utility model. All technical solutions that are the same as or similar to the present utility model are within the scope of protection of the present utility model.

Claims

1. A jellyfish disc-shaped body separation and selection device, characterized in that, The system includes a polyp incubator, a disc-shaped screening pipe, and a circulating filtration device. The polyp incubator has an inlet pipe and an outlet on its lower side wall. The disc-shaped screening pipe consists of multiple screening units connected in series, with a screen mesh at the connection point of adjacent screening units. The mesh size of the multiple screen meshes decreases sequentially along the flow direction. Each screening unit has a sealing cap at its lower part. The circulating filtration device includes a circulating water tank and a variable frequency pump installed within the circulating water tank. The circulating water tank has an inlet on its lower side wall. The outlet of the polyp incubator is connected to the inlet of the disc-shaped screening pipe, and the outlet of the disc-shaped screening pipe is connected to the inlet of the circulating filtration device. The outlet of the variable frequency pump is connected to an outlet pipe, the end of which is connected to the inlet pipe of the polyp incubator.

2. The jellyfish disc-shaped body separation and selection device as described in claim 1, characterized in that, The polyp incubator is cylindrical.

3. The jellyfish disc-shaped body separation and selection device as described in claim 1, characterized in that, The inlet pipe is cylindrical and vertically arranged. The top end of the inlet pipe is located outside the polyp incubator and is connected to the outlet pipe. The bottom end of the inlet pipe is closed and connected to the bottom plate of the polyp incubator. There are multiple small circular holes arranged in a straight line on the side wall of the inlet pipe.

4. The jellyfish disc-shaped body separation and selection device as described in claim 1, characterized in that, The screening unit is cylindrical with sealing flanges installed at both ends, and adjacent screening units are connected by sealing flanges.

5. The jellyfish disc-shaped body separation and selection device as described in claim 1, characterized in that, The circulating water tank contains filter material.

6. The jellyfish disc-shaped body separation and selection device as described in claim 1, characterized in that, The circulating water tank is equipped with a temperature control device.

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