Intestinal flora dynamic culture detector

Abstract

The utility model discloses a kind of intestinal flora dynamic culture detection instruments, comprising: pedestal, box, feeding tank, stirring device, heating device and multistage detection structure.The pedestal upper surface is fixedly connected with box, box top is equipped with feeding tank, feeding tank below is communicated with stirring barrel by through pipe.The first motor is equipped outside stirring barrel, motor drives rotating rod to rotate to drive stirring plate to stir culture solution, rotating rod is equipped with constant-temperature heating rod outside, for maintaining suitable temperature.Culture solution sequentially enters acid-base detection chamber by first downpipe, enters dissolved oxygen detection box by second downpipe, then enters dynamic monitoring chamber by third downpipe, and the detection of pH value, dissolved oxygen and flora state is realized gradually.Dynamic monitoring chamber top is equipped with exhaust duct connection air pump, air pump introduces waste gas into waste gas treatment tank through first air pipe, realizes waste gas purification discharge.The device structure is compact, function is complete, can realize dynamic culture and multi-parameter monitoring to intestinal flora.

Description

Technical Field

[0001] This utility model relates to the field of microbial detection and bioreactor technology, specifically to an instrument for dynamic culture and detection of intestinal flora. Background Technology

[0002] A dynamic gut microbiota culture and detection instrument is used for the in vitro dynamic culture and environmental simulation detection of gut microbiota. It is primarily used to simulate the internal conditions of the human gut, such as temperature, pH, and dissolved oxygen, to achieve continuous monitoring of the growth, metabolism, and changes in the microbiota. It is suitable for applications such as medical research, drug screening, and gut microbiota regulation. Traditional gut microbiota culture methods mostly employ static culture techniques, which are difficult to simulate the dynamic and complex environment within the human gut, such as temperature changes, pH fluctuations, and anaerobic conditions. Furthermore, the detection methods are limited, failing to achieve real-time monitoring of the microbiota status. Simultaneously, gases generated during the culture process, if not effectively treated, can easily affect experimental results and operational safety. Therefore, there is an urgent need for a dynamic gut microbiota culture and detection device that can dynamically regulate the culture environment, achieve simultaneous monitoring of multiple parameters, and has waste gas treatment capabilities to meet the needs of scientific and clinical research. Utility Model Content

[0003] The purpose of this invention is to solve at least one of the technical problems existing in the prior art by providing a dynamic intestinal flora culture and detection instrument. This instrument optimizes existing methods for flora culture, addressing issues such as inaccurate environmental control, limited detection parameters, and non-standard waste gas emissions. Through the cooperation of a feeding tank and a stirring tank, continuous addition and uniform stirring of the culture medium are achieved. A constant temperature is maintained by a constant-temperature heating rod, simulating the intestinal microenvironment. The acid-base detection chamber, dissolved oxygen detection chamber, and dynamic monitoring chamber are set up in a tiered manner, enabling simultaneous monitoring of pH, dissolved oxygen, and flora status, thus improving the comprehensiveness and accuracy of detection. An air pump and waste gas treatment chamber effectively remove waste gas during the culture process, ensuring a stable internal environment within the monitoring chamber and preventing interference with detection results, thereby improving the reliability and repeatability of the culture.

[0004] This utility model also provides an intestinal flora dynamic culture and detection instrument as described above, comprising: a base, a box fixedly connected to the upper surface of the base, a feeding box fixedly connected to the upper surface of the box, a straight pipe fixedly connected to the lower surface of the feeding box, a stirring tank fixedly connected to the lower surface of the straight pipe, a first motor fixedly connected to the outer surface of the stirring tank, a first rotating rod fixedly connected to the output end of the first motor, a stirring plate fixedly connected to the outer surface of the first rotating rod, a constant temperature heating rod fixedly connected to the outer surface of the first rotating rod, a first falling pipe fixedly connected to the lower surface of the stirring tank, an acid-base detection chamber fixedly connected to the lower surface of the first falling pipe; a second falling pipe fixedly connected to the lower surface of the acid-base detection chamber, a dissolved oxygen detection box fixedly connected to the lower surface of the second falling pipe, a third falling pipe fixedly connected to the lower surface of the dissolved oxygen detection box, a dynamic monitoring chamber fixedly connected to the lower surface of the third falling pipe, an exhaust duct fixedly connected to the upper surface of the dynamic monitoring chamber, an air pump fixedly connected to the end of the exhaust duct, a first air guide pipe fixedly connected to the rear surface of the air pump, and a waste gas treatment box fixedly connected to the side surface of the first air guide pipe. The above components contribute to the stability of the dynamic monitoring room.

[0005] According to the intestinal flora dynamic culture and detection instrument provided by this utility model, a support leg is fixedly connected to the lower surface of the base, and a support pad is provided on the lower surface of the support leg. These components help to better secure the entire detection instrument, ensuring its normal operation.

[0006] According to the intestinal flora dynamic culture and detection instrument provided by this utility model, a first mounting port is fixedly connected to the side surface of the housing, and an observation window is fixedly connected to the inner surface of the first mounting port. These components facilitate observation of the internal vibration and sieving process of the device.

[0007] According to the intestinal flora dynamic culture and detection instrument provided by this utility model, a first fixing block is fixedly connected to the side surface of the box, and a brush is fixedly connected to the inner surface of the first fixing block. These components facilitate the cleaning of dust from the device.

[0008] According to the intestinal flora dynamic culture and detection instrument provided by this utility model, a first support rod is fixedly connected to the lower surface of the acid-base detection chamber, and the first support rod is fixedly connected to the bottom surface of the box. These components help to support the acid-base detection chamber and ensure its normal operation.

[0009] According to the intestinal flora dynamic culture and detection instrument provided by this utility model, a sealing door is rotatably connected to the front surface of the housing, and a rotating handle is fixedly connected to the outer surface of the sealing door. These components help to increase the sealing performance of the device and ensure the production quality of the detection instrument.

[0010] According to the intestinal flora dynamic culture and detection instrument provided by this utility model, a heat-insulating pad is fixedly connected to the side surface of the dynamic monitoring chamber. These components help reduce heat loss and improve heating efficiency.

[0011] According to the intestinal flora dynamic culture and detection instrument provided by this utility model, a sealing ring is fixedly connected to the outer surface of the first air guide tube, and the rear surface of the sealing ring is fixedly connected to the exhaust gas treatment box. These components help prevent gas leakage and air pollution.

[0012] Compared with existing technologies, this intestinal flora dynamic culture and detection instrument achieves automatic addition and mixing of culture medium by setting up a feeding box, a straight pipe, and a stirring tank, ensuring the uniformity of the bacterial culture; the first motor drives the stirring plate to operate, and together with the constant temperature heating rod, it can continuously and stably simulate the intestinal environment; the hierarchical setting of the acid-base detection chamber, dissolved oxygen detection chamber, and dynamic monitoring chamber can effectively realize real-time monitoring of multiple parameters such as pH value, dissolved oxygen, and growth status of the bacterial flora; the exhaust pipe, air pump, and waste gas treatment box work together to effectively discharge and purify the waste gas generated during the experiment, improve the system stability and environmental friendliness, and are suitable for in vitro simulation research of intestinal flora. Attached Figure Description

[0013] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0014] Figure 1 This is a front view of the intestinal flora dynamic culture and detection instrument of this utility model;

[0015] Figure 2 This is a perspective structural diagram of the intestinal flora dynamic culture and detection instrument of this utility model;

[0016] Figure 3 This is a side view of the intestinal flora dynamic culture and detection instrument of this utility model;

[0017] Figure 4 This is a rear view of the intestinal flora dynamic culture and detection instrument of this utility model.

[0018] Legend:

[0019] 1. Base; 2. Box body; 3. Feeding box; 4. Straight pipe; 5. Mixing tank; 6. First motor; 7. First rotating rod; 8. Mixing plate; 9. Constant temperature heating rod; 10. First drop pipe; 11. Acid-base detection chamber; 12. Second drop pipe; 13. Dissolved oxygen detection chamber; 14. Third drop pipe; 15. Dynamic monitoring chamber; 16. Exhaust duct; 17. Air pump; 18. First air guide pipe; 19. Waste gas treatment box; 20. Support leg; 21. Support pad; 22. First mounting port; 23. Observation window; 24. First fixing block; 25. Brush; 26. First support rod; 27. Sealing door; 28. Rotating handle; 29. ​​Insulation pad; 30. Sealing ring. Detailed Implementation

[0020] This section will describe in detail the specific embodiments of the present utility model. The preferred embodiments of the present utility model are shown in the accompanying drawings. The purpose of the drawings is to supplement the textual description with graphics, so that people can intuitively and vividly understand each technical feature and overall technical solution of the present utility model, but they should not be construed as limiting the scope of protection of the present utility model.

[0021] Reference Figures 1-4 The intestinal flora dynamic culture and detection instrument of this utility model includes: a base 1, a housing 2 fixedly connected to the upper surface of the base 1, a first mounting port 22 fixedly connected to the side surface of the housing 2, and an observation window 23 fixedly connected to the inner surface of the first mounting port 22. A first fixing block 24 fixedly connected to the side surface of the housing 2, and a brush 25 fixedly connected to the inner surface of the first fixing block 24. A sealing door 27 is rotatably connected to the front surface of the housing 2, and a rotating handle 28 is fixedly connected to the outer surface of the sealing door 27. A feeding box 3 is fixedly connected to the upper surface of the box 2. A straight pipe 4 is fixedly connected to the lower surface of the feeding box 3. A mixing tank 5 is fixedly connected to the lower surface of the straight pipe 4. A first motor 6 is fixedly connected to the outer surface of the mixing tank 5. A first rotating rod 7 is fixedly connected to the output end of the first motor 6. A stirring plate 8 is fixedly connected to the outer surface of the first rotating rod 7. A constant temperature heating rod 9 is fixedly connected to the outer surface of the first rotating rod 7. A first falling pipe 10 is fixedly connected to the lower surface of the mixing tank 5. An acid-base detection chamber 11 is fixedly connected to the lower surface of the first falling pipe 10.

[0022] Specifically, a housing 2 is fixedly connected to the upper surface of the base 1, serving to support and protect the internal structure. A feeding box 3 is fixedly connected to the upper surface of the housing 2, and a straight pipe 4 is provided at the bottom of the feeding box 3 to facilitate the flow of the culture medium. A stirring tank 5 is fixedly connected below the straight pipe 4, and a first motor 6 is installed outside the stirring tank 5. The first motor 6 drives the first rotating rod 7 at its output end to rotate, thereby driving the stirring plate 8 on its exterior to mix the culture medium. A constant temperature heating rod 9 is also provided outside the first rotating rod 7 to maintain the temperature of the culture environment. A first drop pipe 10 is connected to the lower end of the stirring tank 5, through which the culture medium can enter the acid-base detection chamber 11 below to monitor pH changes and achieve dynamic detection function.

[0023] A second drop pipe 12 is fixedly connected to the lower surface of the acid-base detection chamber 11, and a first support rod 26 is fixedly connected to the lower surface of the acid-base detection chamber 11. The first support rod 26 is fixedly connected to the bottom surface of the box body 2. A dissolved oxygen detection chamber 13 is fixedly connected to the lower surface of the second drop pipe 12, and a third drop pipe 14 is fixedly connected to the lower surface of the dissolved oxygen detection chamber 13. A dynamic monitoring chamber 15 is fixedly connected to the lower surface of the third drop pipe 14, and a heat insulation pad 29 is fixedly connected to the side surface of the dynamic monitoring chamber 15. An exhaust duct 16 is fixedly connected to the upper surface of the dynamic monitoring chamber 15, and an air pump 17 is fixedly connected to the end of the exhaust duct 16. A first air guide pipe 18 is fixedly connected to the rear surface of the air pump 17, and a sealing ring 30 is fixedly connected to the outer surface of the first air guide pipe 18. The rear surface of the sealing ring 30 is fixedly connected to the waste gas treatment box 19. The waste gas treatment box 19 is fixedly connected to the side surface of the first air guide pipe 18, and a support leg 20 is fixedly connected to the lower surface of the base 1. A support pad 21 is provided on the lower surface of the support leg 20.

[0024] Specifically, a second drop pipe 12 is fixedly connected to the lower surface of the acid-base detection chamber 11 to guide the liquid into the dissolved oxygen detection chamber 13 for subsequent dissolved oxygen monitoring. A third drop pipe 14 is located on the lower surface of the dissolved oxygen detection chamber 13, connecting to the dynamic monitoring chamber 15 for comprehensive monitoring of bacterial growth. An exhaust duct 16 is fixedly connected to the upper surface of the dynamic monitoring chamber 15 to exhaust internal gas. The end of the exhaust duct 16 is connected to an air pump 17 to provide gas flow power. A first gas guide pipe 18 is connected to the rear surface of the air pump 17 to transfer gas. A waste gas treatment box 19 is located on the side surface of the first gas guide pipe 18 to purify the discharged waste gas and ensure environmental safety.

[0025] Working Principle: Culture medium is added through the feeding tank 3 and flows into the stirring tank 5 via the straight pipe 4. The first motor 6 drives the rotating rod 7 to rotate, which in turn drives the stirring plate 8 to stir the culture medium. Simultaneously, the constant temperature heating rod 9 heats the liquid, simulating the intestinal temperature environment. After stirring, the liquid enters the acid-base detection chamber 11 through the first drop pipe 10 to monitor the pH value in real time, and then enters the dissolved oxygen detection chamber 13 through the second drop pipe 12 to monitor the dissolved oxygen level. Subsequently, the liquid flows into the dynamic monitoring chamber 15 through the third drop pipe 14 for monitoring the bacterial community status. Gases generated during the cultivation process are extracted by the air pump 17 through the exhaust pipe 16 and introduced into the waste gas treatment box 19 for purification and discharge through the first air guide pipe 18, ensuring stable system operation.

[0026] The embodiments of the present utility model have been described in detail above with reference to the accompanying drawings. However, the present utility model is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present utility model.

Claims

1. A dynamic intestinal flora culture and detection instrument, characterized in that, include: A base (1) is fixedly connected to a box (2) on its upper surface. A feeding box (3) is fixedly connected to the upper surface of the box (2). A straight pipe (4) is fixedly connected to the lower surface of the feeding box (3). A stirring tank (5) is fixedly connected to the lower surface of the straight pipe (4). A first motor (6) is fixedly connected to the outer surface of the stirring tank (5). A first rotating rod (7) is fixedly connected to the output end of the first motor (6). A stirring plate (8) is fixedly connected to the outer surface of the first rotating rod (7). A constant temperature heating rod (9) is fixedly connected to the outer surface of the first rotating rod (7). A first falling pipe (10) is fixedly connected to the lower surface of the stirring tank (5). An acid-base detection chamber (11) is fixedly connected to the lower surface of the first falling pipe (10). A second drop pipe (12) is fixedly connected to the lower surface of the acid-base detection chamber (11). A dissolved oxygen detection box (13) is fixedly connected to the lower surface of the second drop pipe (12). A third drop pipe (14) is fixedly connected to the lower surface of the dissolved oxygen detection box (13). A dynamic monitoring chamber (15) is fixedly connected to the lower surface of the third drop pipe (14). An exhaust duct (16) is fixedly connected to the upper surface of the dynamic monitoring chamber (15). An air pump (17) is fixedly connected to the end of the exhaust duct (16). A first air guide pipe (18) is fixedly connected to the rear surface of the air pump (17). A waste gas treatment box (19) is fixedly connected to the side surface of the first air guide pipe (18).

2. The intestinal flora dynamic culture and detection instrument according to claim 1, characterized in that, The base (1) has a support leg (20) fixedly connected to its lower surface, and the support leg (20) has a support pad (21) on its lower surface.

3. The intestinal flora dynamic culture and detection instrument according to claim 1, characterized in that, The side surface of the housing (2) is fixedly connected to a first mounting port (22), and the inner surface of the first mounting port (22) is fixedly connected to an observation window (23).

4. The intestinal flora dynamic culture and detection instrument according to claim 1, characterized in that, A first fixing block (24) is fixedly connected to the side surface of the box (2), and a brush (25) is fixedly connected to the inner surface of the first fixing block (24).

5. The intestinal flora dynamic culture and detection instrument according to claim 1, characterized in that, The lower surface of the acid-base detection chamber (11) is fixedly connected to a first support rod (26), and the first support rod (26) is fixedly connected to the bottom surface of the box body (2).

6. The intestinal flora dynamic culture and detection instrument according to claim 1, characterized in that, The front surface of the box (2) is rotatably connected to a closed door (27), and the outer surface of the closed door (27) is fixedly connected to a rotating handle (28).

7. The intestinal flora dynamic culture and detection instrument according to claim 1, characterized in that, A thermal insulation pad (29) is fixedly connected to the side surface of the dynamic monitoring chamber (15).

8. The intestinal flora dynamic culture and detection instrument according to claim 1, characterized in that, A sealing ring (30) is fixedly connected to the outer surface of the first air guide pipe (18), and the rear surface of the sealing ring (30) is fixedly connected to the exhaust gas treatment box (19).

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