Perfusion infiltration application sample carrier and perfusion module

Abstract

The utility model provides a kind of sample plate of perfusion infiltration application, it includes plate body, at least two groups for carrying biological tissue section sample's sample space of loading are provided on the plate body, each group the sample space is arranged and is separately provided along front-back direction;Each group the sample space one end is respectively communicated and is arranged drain, each group the sample space other end is respectively communicated and is shunted space.Based on the independent design of sample plate, the feasibility that the perfusion module applied thereto can be combined to replace different tissue perfusion sample plate is enabled, so that the perfusion module can be adapted to be combined according to biological tissue section sample, tissue selection different sample plate, liquid inlet plate, meet the perfusion infiltration demand of external perfusion solution to biological tissue section sample.And heat transfer is realized by temperature control plate, liquid inlet plate realizes liquid inlet and shunt, can guarantee the consistency, repeatability of perfusion operation.

Description

Technical Field

[0001] This utility model relates to the field of biological tissue section sample perfusion technology, specifically, to a sample carrier and perfusion module for perfusion infiltration application. Background Technology

[0002] The perfusion of biological tissue sections under in vitro conditions directly affects the cell viability of the sections during subsequent cryopreservation, depending on factors such as appropriate temperature control, sufficient tissue section infiltration, and stable perfusion solution flow. Existing perfusion devices suffer from challenges such as difficulty in standardizing perfusion conditions, large fluctuations in experimental results, and difficulty in controlling experimental reproducibility and consistency. These factors significantly impact the cell viability of cryopreserved biological tissue sections. Therefore, improving the stability and reproducibility of perfusion has been a pressing issue in this field. Utility Model Content

[0003] To overcome the shortcomings of the prior art, this utility model provides a carrier plate and irrigation module for irrigation and wetting applications.

[0004] A sample carrier plate for perfusion infiltration application includes a plate body, on which at least two sets of sample carrier spaces for carrying biological tissue slice samples are provided. Each set of sample carrier spaces is arranged and separated along the front-to-back direction. One end of each set of sample carrier spaces is connected to a drain port, and the other end of each set of sample carrier spaces is connected to a diversion space.

[0005] Furthermore, each of the sample carrying spaces includes at least two sample carrying cavities, which are connected by an immersion channel; the cross-section of each sample carrying cavity is elliptical, the length of each sample carrying cavity is along the front-back direction, and the immersion channel is connected to the two ends of the length direction of two adjacent sample carrying cavities.

[0006] Furthermore, the immersion channel includes a horizontally connected straight channel at the length end between two adjacent sample loading chambers, and / or the immersion channel includes an outlet located on the upper side of one end of one sample loading chamber, an inlet located on the lower side of the other end of the other sample loading chamber, and a connecting channel connecting the outlet and the inlet; the outlet and the inlet have a height difference.

[0007] Furthermore, the diversion space includes a first diversion cavity, the extension width of which corresponds to a width greater than or equal to the side-by-side arrangement width of the two sets of sample-carrying spaces, and the sample-carrying spaces within the extension width range of the first diversion cavity are connected to the first diversion cavity; and / or, the diversion space includes a second diversion cavity, the second diversion cavity being set and connected to the position of a single set of sample-carrying spaces.

[0008] Furthermore, it also includes a substrate, wherein the sample carrying space and the flow distribution space of the plate are vertically connected, and the substrate is placed on the lower side of the plate and forms a closure on the lower side of the sample carrying space and the flow distribution space.

[0009] The perfusion module includes a sample carrier plate as described above; a liquid inlet plate is pressed onto the upper side of the sample carrier plate to form a closed upper side for each of the sample carrier spaces; and a liquid inlet channel is provided on the liquid inlet plate to connect the outside world with the sample carrier space.

[0010] Furthermore, the liquid inlet channel includes a liquid inlet, a liquid outlet, and a liquid inlet passage connecting the liquid inlet and the liquid outlet on the liquid inlet plate; the liquid outlet is provided corresponding to the diversion space.

[0011] Furthermore, the liquid inlet is located on the outer periphery of the liquid inlet plate, the liquid inlet channel includes a horizontally extending cross channel on the liquid inlet plate, the cross channel is provided with a flow channel outlet, at least two liquid inlet outlets are provided corresponding to each group of sample carrying spaces and located on the lower side of the liquid inlet plate, and at least two liquid inlet branch channels are provided between the flow channel outlet and each liquid inlet outlet in a diverted manner;

[0012] Furthermore, an independently controlled control valve is provided in the liquid inlet channel corresponding to at least one set of sample-carrying space positions.

[0013] Furthermore, it also includes a temperature control plate, which is disposed on the lower side of the sample carrier plate and forms a heat exchange relationship with each of the sample carrier spaces; the front side of the temperature control plate is provided with a downward-sloping drainage ramp corresponding to the position of the drainage port.

[0014] The beneficial effects of this utility model are as follows:

[0015] Based on the independent design of the sample carrier, the perfusion module using this technology allows for the combination and replacement of sample carriers for different tissue perfusions. This enables the perfusion module to be adapted to combine different sample carriers and infusion plates according to the biological tissue section sample and the tissue itself, meeting the perfusion and wetting requirements of the external perfusion solution for the biological tissue section sample. Furthermore, heat transfer is achieved through a temperature control plate, and infusion and diversion are achieved through the infusion plate, ensuring the consistency and repeatability of the perfusion operation.

[0016] The perfusion module features outlets and inlets with varying heights in each sample chamber on the sample carrier plate. This design ensures that the perfusion solution fully infiltrates the biological tissue slices in the sample chambers as it passes through the perfusion module, guaranteeing effective infiltration.

[0017] The perfusion module, with its multiple separate sample-carrying spaces and independent control valves in the inlet channel, can simultaneously perform batch perfusion of multiple biological tissue slices, or allow a small number of biological tissue slices to be independently perfused in a single sample-carrying space. This allows the perfusion module to meet the control requirements for perfusion and perfusion of different numbers of biological tissue slices, and to achieve reasonable distribution and control of the perfusion solution. Attached Figure Description

[0018] Figure 1 This is an exploded structural diagram of the irrigation module in Example 1;

[0019] Figure 2 yes Figure 1 A magnified view of part A;

[0020] Figure 3 This is a cross-sectional structural diagram of the irrigation module in Example 1;

[0021] Figure 4 yes Figure 3 A magnified view of part B;

[0022] Figure 5 This is an exploded structural diagram of the irrigation module in Example 2;

[0023] Figure 6 This is a cross-sectional structural diagram of the irrigation module in Example 2.

[0024] Explanation of reference numerals in the attached figures:

[0025] Sample carrier 1; plate body 10, substrate 101, sample carrying space 11, sample carrying cavity 111, immersion channel 12, outlet 121, connecting channel 122, connecting cavity 1221, first channel 1222, second channel 1223, inlet 123, drain 13, diversion space 14, first diversion cavity 141, second diversion cavity 142, positioning wall 15, positioning sealing plate 16, diversion port 161.

[0026] Liquid inlet plate 2; liquid inlet channel 21, liquid inlet 22, liquid inlet outlet 23, liquid inlet passage 24, crossflow channel 241, channel outlet 242, liquid inlet branch channel 243.

[0027] Control valve 3; slotted valve 31

[0028] Temperature control plate 4; drainage ramp 41. Detailed Implementation

[0029] To make the technical solution, purpose and advantages of this utility model clearer, the following explanation is given in conjunction with the accompanying drawings and embodiments.

[0030] like Figures 1 to 6As shown, this utility model provides a perfusion module for the application of perfusion of biological tissue section samples. The perfusion module includes a sample carrier plate 1 and a liquid inlet plate 2; the sample carrier plate 1 is provided with at least two sets of sample carrying spaces 11 for carrying biological tissue section samples. The lower side and outer periphery of the sample carrying space 11 are closed and form an upper opening for placing the biological tissue section sample. One end of each set of sample carrying spaces 11 is respectively connected to a drain port 13. The liquid inlet plate 2 is pressed against the upper side of the sample carrier plate 1, so that the upper side of each sample carrying space 11 is closed; the liquid inlet plate 2 is provided with a liquid inlet channel 21 connecting the outside and the sample carrying space 11.

[0031] By setting up the sample carrier plate 1 and the liquid inlet plate 2 separately, different combinations of sample carrier plate 1 and liquid inlet plate 2 can be made to meet the application of sample carrier plate 1 with different arrangement and connection methods of sample space 11 and liquid inlet plate 2 with different liquid inlet organization according to the actual needs of the perfusion module.

[0032] Specifically, the sample carrier plate 1 is designed to include a plate body 10, which is rectangular in shape and arranged with its length direction as the front-to-back direction. At least two sets of sample carrying spaces 11 for carrying biological tissue slice samples are provided on the plate body 10. Each set of sample carrying spaces 11 is arranged and separated along the front-to-back direction. The front end of each set of sample carrying spaces 11 is respectively connected to a drain port 13, and the rear end of each set of sample carrying spaces 11 is respectively connected to a diversion space 14.

[0033] Each group of sample-carrying spaces 11 includes at least two sample-carrying cavities 111, which are connected by an immersion channel 12. Multiple sample-carrying cavities 111 are arranged in a group along the horizontal direction on the sample-carrying plate 1, and the immersion channel 12 is arranged in a front-to-back direction between two adjacent sample-carrying cavities 111 in the same group. The cross-section of each sample-carrying cavity 111 is elliptical, and the length of each sample-carrying cavity 111 is along the front-to-back direction. The immersion channel 12 connects the two ends of the length direction of two adjacent sample-carrying cavities 111.

[0034] In a preferred embodiment, the perfusion module further includes a temperature control plate 4, which is disposed on the lower side of the sample carrier plate 1 and forms a heat exchange relationship with each of the sample carrier spaces 11, allowing for the connection of an external heating or cooling module for temperature control and heat exchange applications of the perfusion module. A downward-sloping drainage ramp 41 is provided on the front side of the temperature control plate 4 corresponding to the drain port 13. The drainage ramp 41 facilitates the discharge of the tissue perfusion solution.

[0035] Example 1:

[0036] This embodiment addresses the configuration of an irrigation module to ensure effective wetting. The sample carrier 1 in this embodiment is configured as follows: the wetting channel 12 includes an outlet 121 located on the upper side of one end of a sample carrier cavity 111, an inlet 123 located on the lower side of the other end of a sample carrier cavity 111, and a connecting channel 122 linking the outlet 121 and the inlet 123; the outlet 121 and the inlet 123 have a height difference; the outlet 121 and the inlet 123 are respectively located on opposite sides of the length of the sample carrier cavity 111. A cylindrical vertically arranged connecting cavity 1221 is provided between two adjacent sample-carrying cavities 111. One end of the connecting cavity 1221 extends horizontally to form a first flow channel 1222 that communicates with the outlet 121 of one sample-carrying cavity 111. The other end of the connecting cavity 1221 extends horizontally to form a second flow channel 1223 that communicates with the inlet 123 of another sample-carrying cavity 111. The first flow channel 1222, the connecting cavity 1221, and the second flow channel 1223 form the connecting channel 122.

[0037] Through the specific configuration of the above-mentioned immersion channel 12 and the arrangement of the grouped sample loading cavities 111, it can be ensured that during the immersion process, the immersion solution can flow from back to front according to the grouped sample loading cavities 111, thus ensuring the fluidity and flow efficiency of the immersion; at the same time, it ensures that the immersion solution fills each sample loading cavity 111, thus ensuring the immersion effect.

[0038] The diversion space 14 includes a first diversion cavity 141. The extension width of the first diversion cavity 141 corresponds to a width greater than or equal to the side-by-side arrangement width of the two sets of sample-carrying spaces 11. The sample-carrying spaces 11 within the extension width range of the first diversion cavity 141 are connected to the first diversion cavity 141. An externally input perfusion solution is input through the inlet channel 21 to fill the first diversion cavity 141. Based on the fact that the inlet 123 located at the rear side of the first diversion cavity 141 and each set of sample-carrying spaces 111 are at the same horizontal position, the perfusion solution will be continuously delivered to each set of sample-carrying spaces 111 at equal pressure, so as to meet the perfusion and infiltration requirements of biological tissue slice samples in multiple sets of sample-carrying spaces 111 in a single input, and effectively ensure that the perfusion effect between each set of sample-carrying spaces 111 is equal.

[0039] The liquid inlet channel 21 includes a liquid inlet 22, a liquid inlet 23, and a liquid inlet channel 24 connecting the liquid inlet 22 and the liquid inlet 23, all disposed on the liquid inlet plate 2. The liquid inlet 22 is disposed on the outer periphery of the liquid inlet plate 2. The liquid inlet channel 24 includes a horizontally extending cross channel 241 on the rear side of the liquid inlet plate 2. A channel outlet 242 is disposed on the cross channel 241. The channel outlet 242 is disposed at the middle position of each group of sample-carrying cavities 111. Multiple liquid inlet outlets 23 are evenly distributed and disposed on the lower side of the liquid inlet plate 2. Multiple liquid inlet branch channels 243 are evenly distributed and diverted between the channel outlets 242 and each liquid inlet outlet 23. Each liquid inlet outlet 23 is disposed facing the arrangement range of the first diversion cavity 141. Each liquid inlet outlet 23 is disposed at the rear end position of each group of sample-carrying spaces 11.

[0040] Example 2:

[0041] In this embodiment, for an immersion module that can autonomously select a specific sample space 11 for perfusion operation, multiple independently controlled control valves 3 are provided in the liquid inlet channel 21 corresponding to each group of sample spaces 11.

[0042] Specifically, the diversion space 14 includes a second diversion cavity 142, which is located and connected to the rear side of the single sample carrying space 11; the liquid inlet channel 21 of this embodiment includes a liquid inlet 22, a liquid outlet 23 and a liquid inlet channel 24 connecting the liquid inlet 22 and the liquid outlet 23, which are provided on the liquid inlet plate 2; the liquid inlet 22 is located on the outer periphery of the liquid inlet plate 2, and the liquid inlet channel 24 includes a horizontally extending cross channel 241 on the rear side of the liquid inlet plate 2, a channel outlet 242 is provided on the cross channel 241, and multiple liquid inlet branch channels 243 are evenly distributed and diverted between the channel outlet 242 and each liquid inlet outlet 23; multiple liquid inlet outlets 23 are provided and located on the lower side of the liquid inlet plate 2, corresponding to each of the second diversion cavities 142; each of the control valves 3 is connected to each of the liquid inlet branch channels 243 and the output end of each of the control valves 3 is provided to each of the liquid inlet outlets 23.

[0043] The inlet channel 21 is branched into multiple inlet branch channels 243, connecting to the outside and each of the second diversion chambers 142. The output end of each control valve 3 is set corresponding to each of the second diversion chambers 142. Through the application of the second diversion chambers 142 and the control valves 3, the perfusion solution input to each sample carrying space 11 can be kept stable, while ensuring that each sample carrying space 11 has a corresponding independent control valve 3 to control the start and stop of the perfusion solution input.

[0044] In this embodiment, the control valve 3 is located on the upper side of the inlet plate 2 with a control end for controlling the opening and closing of the control valve 3. Specifically, the control end is provided with a slot 31, and the valve structure is raised and lowered by rotating the slot 31 with a screwdriver to realize the opening and closing of the control valve 3.

[0045] In the same group of sample-carrying spaces 11, multiple sample-carrying cavities 111 are arranged in a straight line along their length, and adjacent sample-carrying cavities 111 are connected by wetting channels 12; the wetting channels 12 include a straight connecting channel arranged at the length end between two adjacent sample-carrying cavities 111. Through the arrangement of the grouped sample-carrying spaces 11, it is ensured that during the perfusion wetting process, the perfusion solution can flow from back to front through the grouped sample-carrying spaces 11, ensuring the fluidity and flow efficiency of the perfusion wetting; at the same time, it ensures that the perfusion solution fills each sample-carrying space 11, ensuring the perfusion wetting effect. Each of the multiple groups of sample-carrying spaces 11 is equipped with a corresponding control valve 3. The opening and closing of the control valve 3 controls the start and stop of the perfusion solution entering the corresponding sample-carrying space 11, meeting the need for on-demand control of the input perfusion solution to a specific sample-carrying space 11.

[0046] Each sample-carrying space 11 is surrounded by a positioning wall 15; it also includes a positioning sealing plate 16, which is adapted to be inserted into the positioning wall 15 to form an upper enclosure of the sample-carrying space 11; the positioning sealing plate 16 is set one-to-one with each sample-carrying space 11; the enclosing positioning wall 15 is rectangular in shape, each positioning sealing plate 16 is rectangular in shape, and each positioning sealing plate 16 is provided with a diversion port 161 corresponding to each diversion cavity.

[0047] This embodiment and the above embodiment 1 can be arranged and applied in a comprehensive manner according to actual needs, including the first diversion cavity 141, the second diversion cavity 142, the sample carrying space 11 connected by a straight channel, and the sample carrying space 11 connected by an outlet 121 with a height difference and an inlet 123; and an independently controlled control valve 3 is set in the liquid inlet channel 21 corresponding to at least one set of sample carrying spaces 11.

[0048] Example 3:

[0049] In this embodiment, in order to improve the modular design of the irrigation module, a combined design scheme of the carrier template 1 is further provided for application.

[0050] like Figure 1 As shown, the sample carrier plate 1 also includes a substrate 101. The sample carrying space 11 and the flow distribution space 14 of the plate body 10 are connected vertically. The substrate 101 is placed on the lower side of the plate body 10 and forms a closure on the lower side of the sample carrying space 11 and the flow distribution space 14.

[0051] The plate 10 can be made of elastomeric materials such as silicone through compression molding, which has low production cost. When used in sterile scenarios such as medical care, it can be used as a disposable consumable, which is convenient for large-scale production and replacement.

[0052] The substrate 101 can be made of metal materials such as stainless steel, which has high thermal conductivity, making it convenient to use with the temperature control plate 4 to control the temperature during the perfusion process. It can also be reused more than 10 times with the consumable board body through high-temperature sterilization / sterilization.

[0053] The above description is only a preferred embodiment of the present utility model. For those skilled in the art, modifications can still be made to the embodiments without departing from the implementation principle of the present utility model, and the corresponding modifications should also be considered within the protection scope of the present utility model.

Claims

1. A sample plate for irrigation and wetting applications, characterized in that, The plate includes a plate body, on which at least two sets of sample-carrying spaces for carrying biological tissue slice samples are provided. Each set of sample-carrying spaces is arranged and separated along the front-back direction. One end of each set of sample-carrying spaces is connected to a drain port, and the other end of each set of sample-carrying spaces is connected to a diversion space.

2. The carrier plate as described in claim 1, characterized in that, Each sample carrying space includes at least two sample carrying cavities, which are connected by an immersion channel. The cross-section of each sample carrying cavity is elliptical, and the length of each sample carrying cavity is along the front-to-back direction. The immersion channel connects the two ends of the length direction of two adjacent sample carrying cavities.

3. The carrier plate as described in claim 2, characterized in that, The immersion channel includes a horizontally connected straight channel at the length end between two adjacent sample loading chambers, and / or the immersion channel includes an outlet located on the upper side of one end of one sample loading chamber, an inlet located on the lower side of the other end of the other sample loading chamber, and a connecting channel connecting the outlet and the inlet; the outlet and the inlet have a height difference.

4. The carrier plate as described in claim 1, characterized in that, The diversion space includes a first diversion cavity, the extension width of which corresponds to a width greater than or equal to the side-by-side arrangement width of the two sets of sample carrying spaces, and the sample carrying spaces within the extension width range of the first diversion cavity are connected to the first diversion cavity; and / or, the diversion space includes a second diversion cavity, the second diversion cavity being set and connected to the position of a single set of sample carrying spaces.

5. The carrier plate as described in claim 1, characterized in that, It also includes a substrate, wherein the sample carrying space and the flow distribution space of the plate are connected vertically, and the substrate is placed on the lower side of the plate and forms a closure on the lower side of the sample carrying space and the flow distribution space.

6. An irrigation module, characterized in that, include: The sample carrier as described in any one of claims 1 to 5; The liquid inlet plate is pressed against the upper side of the sample carrier plate, so that the upper side of each sample carrier space is closed; the liquid inlet plate is provided with a liquid inlet channel connecting the outside world and the sample carrier space.

7. The irrigation module as described in claim 6, characterized in that, The liquid inlet channel includes a liquid inlet, a liquid outlet, and a liquid inlet passage connecting the liquid inlet and the liquid outlet on the liquid inlet plate; the liquid outlet is provided corresponding to the diversion space.

8. The irrigation module as described in claim 7, characterized in that, The liquid inlet is located on the outer periphery of the liquid inlet plate. The liquid inlet channel includes a horizontally extending cross channel on the liquid inlet plate. A flow channel outlet is provided on the cross channel. At least two liquid inlet outlets are provided corresponding to each group of sample carrying spaces and are located on the lower side of the liquid inlet plate. At least two liquid inlet branch channels are provided between the flow channel outlet and each of the liquid inlet outlets to divert the flow.

9. The irrigation module as described in claim 7, characterized in that, The liquid inlet channel is equipped with an independently controlled control valve corresponding to at least one set of sample-carrying space positions.

10. The irrigation module as described in claim 6, characterized in that, It also includes a temperature control plate, which is disposed on the lower side of the sample carrier plate and forms a heat exchange relationship with each of the sample carrier spaces; the front side of the temperature control plate is provided with a downward-sloping drainage ramp corresponding to the position of the drainage port.

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