An ultrasonic fixation device for extracting an antigen

Abstract

The utility model provides an ultrasonic fixing device for extracting antigen, and particularly relates to the field of antigen extraction, and comprises a temperature controller, an upper opening type ice box for placing ice blocks, first moving plates and second moving plates symmetrically arranged on the upper side of the ice box, recessed cross sections of the first moving plates and the second moving plates, sliding grooves arranged on the two sides of the ice box, the lower ends of the first moving plates and the second moving plates embedded in the sliding grooves, arc-shaped grooves for fixing beakers arranged on the upper end faces of the first moving plates and the second moving plates, rotating plates arranged on the side faces of the first moving plates, the rotating plates rotatably connected to the first moving plates at one end, semicircular positioning grooves arranged on the other end of the rotating plates, and positioning pins matched with the positioning grooves arranged on the side faces of the second moving plates. The utility model solves the problems of beaker sliding and probe displacement during ultrasonic treatment, ensures the maximum ultrasonic lysis efficiency of cells, and improves the yield of antigen.

Description

Technical Field

[0001] This utility model belongs to the field of antigen extraction, and specifically relates to an ultrasonic fixation device for extracting antigens. Background Technology

[0002] Extracting CA199 antigen from cells typically requires lysing the cells using ultrasound. This process generates a significant amount of heat, so the cells must be kept in ice water during ultrasound treatment to help dissipate heat and prevent the CA199 antigen from being inactivated at high temperatures.

[0003] In standard procedure, a beaker containing the cell suspension is placed in an ice box, which is then filled with crushed ice to secure the beaker. Next, the ultrasound probe is placed in the center of the beaker, and ultrasonic lysis begins.

[0004] However, during ultrasound, the cell suspension inside the beaker heats up, causing the ice around the beaker to melt. Once the ice melts, the beaker cannot be fixed and may slide outwards. The ultrasound probe, which was originally centered, then touches the beaker wall, significantly reducing the effectiveness of ultrasound lysis and resulting in insufficient cell lysis, which in turn affects the final CA199 antigen yield. Utility Model Content

[0005] The purpose of this invention is to provide an ultrasonic fixation device for extracting antigens, which ensures that when the ice around the beaker melts during the ultrasonic process, the beaker will not move with the ice water, thus ensuring that the ultrasonic probe is always located in the center of the beaker and maximizing the ultrasonic efficiency.

[0006] This utility model provides the following technical solution:

[0007] An ultrasonic fixation device for extracting antigens includes an open-top ice box for holding ice cubes.

[0008] The ice box is symmetrically provided with a first movable plate and a second movable plate on its upper side, and the cross-sections of the first movable plate and the second movable plate are concave.

[0009] The ice box has sliding grooves on both sides, and the lower ends of the first and second movable plates are embedded in the sliding grooves.

[0010] The upper surfaces of the first and second movable plates are provided with arc-shaped grooves for fixing the beaker;

[0011] The first movable plate has a rotating plate on its side, one end of which is rotatably connected to the first movable plate; the other end of the rotating plate has a semi-circular positioning groove, and the second movable plate has a positioning pin on its side that matches the positioning groove.

[0012] Preferably, the rotating plate is provided with a number of positioning grooves in sequence.

[0013] Preferably, the ice box has pins at the four corners of the top, and two rubber bands connect the two pins at opposite corners of the ice box, with the beaker located at the center of the intersection of the rubber bands;

[0014] Preferably, the lower end of the pin is tapered, and the upper end of the pin is provided with an annular groove for embedding the rubber band.

[0015] Preferably, the lower end of the pin passes through the sliding groove to block and limit the first and second moving plates.

[0016] Preferably, the first and second movable plates are provided with several arc-shaped grooves of different diameters.

[0017] Preferably, a buffer pad is provided on the inner side of the arc-shaped groove.

[0018] Preferably, a magnet is embedded in the end face of the arc-shaped groove.

[0019] The beneficial effects of this utility model are:

[0020] This invention has a simple structure and solves the problems of beaker sliding and probe displacement during ultrasound, ensuring maximum efficiency of cell lysis by ultrasound and improving the yield of CA19-9 antigen. Attached Figure Description

[0021] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0022] Figure 1 This is a schematic diagram of the overall structure of the first embodiment;

[0023] Figure 2 This is an exploded view of the first embodiment;

[0024] Figure 3 This is a partial schematic diagram of the first embodiment;

[0025] Figure 4 This is a schematic diagram of the second embodiment.

[0026] The following are marked in the diagram: 1. Ice box; 2. First moving plate; 3. Second moving plate; 4. Arc groove; 5. Sliding groove; 6. Rotating plate; 7. Positioning pin; 8. Pin shaft; 9. Magnet; 10. Buffer pad; 11. Beaker; 12. Rubber band. Detailed Implementation

[0027] like Figures 1 to 3 An ultrasonic fixation device for extracting antigens includes an open-top ice box 1 for holding ice cubes. A first movable plate 2 and a second movable plate 3 are symmetrically arranged on the upper side of the ice box 1. The first movable plate 2 and the second movable plate 3 have concave cross-sections. For details, see [link to specific structure]. Figure 3Meanwhile, the ice box 1 has sliding grooves 5 on both sides. The lower ends of the first moving plate 2 and the second moving plate 3 are embedded in the sliding grooves 5, allowing the first moving plate 2 and the second moving plate 3 to move back and forth within the sliding grooves 5. To ensure smooth sliding, the ice box 1, the first moving plate 2, and the second moving plate 3 are all integrally molded from polytetrafluoroethylene (PTFE). PTFE has good non-stick properties, which can reduce sliding resistance. At the same time, the upper surfaces of the first moving plate 2 and the second moving plate 3 are provided with arc-shaped grooves 4 for fixing the beaker 11. The arc-shaped grooves 4 can lock and limit the two sides of the beaker. In order to ensure the stability of the engagement of the first moving plate 2 and the second moving plate 3, a rotating plate 6 is provided on the side of the first moving plate 2. One end of the rotating plate 6 is rotatably connected to the first moving plate 2 via a shaft. The other end of the rotating plate 6 is provided with a semi-circular positioning groove. The side of the second moving plate 3 is provided with a positioning pin 7 that matches the positioning groove. See the appendix for details. Figure 2 The structure includes a rotating plate 6 that can engage and disengage with the positioning pin 7, thus providing a limiting function. The rotating plate 6 also has several positioning grooves, the specific size and number of which depend on the size of the beaker 11 to be fitted. By engaging the positioning pin 7 with the positioning grooves at different positions on the rotating plate 6, the distance between the first moving plate 2 and the second moving plate 3 can be changed, thus accommodating beakers 11 of different sizes. To reduce hard contact, a buffer pad 10 is provided on the inner side of the arc-shaped groove 4, which can be connected to the arc-shaped groove 4 by adhesive. Furthermore, to accommodate beakers 11 of more sizes, the first moving plate 2 and the second moving plate 3 have several arc-shaped grooves 4 of different diameters, increasing the fit range.

[0028] To ensure the stability of the first movable plate 2 and the second movable plate 3, pins 8 are provided at the four corners of the upper end of the ice box 1. The lower ends of the pins 8 pass through the sliding grooves 5 to block and limit the movement of the first movable plate 2 and the second movable plate 3. See the appendix for details. Figure 1 Structure: A magnet 9 is embedded in the end face of the arc-shaped groove 4. When the size of the arc-shaped groove 4 matches the diameter of the beaker 11, it is attracted by the magnet 9 on the end face of the first moving plate 2 and the second moving plate 3, which helps to improve the stability of the fixation. When the ice around the beaker 11 melts during the ultrasound process, the beaker 11 will not move with the ice water, which can ensure that the ultrasound probe is always located in the center of the beaker 11, ensuring the maximum ultrasound efficiency, the maximum efficiency of cell ultrasound lysis, and the improvement of antigen yield. Example

[0029] like Figure 4 To further improve the fixation reliability, two diagonal pins 8 of the ice box 1 are connected by two rubber bands 12, with the beaker 11 located at the center of the intersection of the rubber bands 12; this can further improve the reliability of the beaker 11; at the same time, the lower end of the pin 8 is tapered, which facilitates the insertion and engagement of the pin 8, and the upper end of the pin 8 is provided with an annular groove for the rubber band 12 to be embedded, which helps to improve the fixation reliability of the rubber band 12.

[0030] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An ultrasonic fixation device for extracting an antigen, characterized by: The ice box comprises an upper opening type ice box for placing ice blocks; The upper side of the ice box is symmetrically provided with a first moving plate and a second moving plate; The first moving plate and the second moving plate are concave in cross section; The ice box is provided with sliding grooves on both sides, and the lower ends of the first moving plate and the second moving plate are embedded in the sliding grooves; The upper end faces of the first moving plate and the second moving plate are provided with arc-shaped grooves for fixing beakers; The side face of the first moving plate is provided with a rotating plate, one end of the rotating plate is rotatably connected with the first moving plate, the other end of the rotating plate is provided with a semicircular positioning groove, and the side face of the second moving plate is provided with a positioning pin matched with the positioning groove.

2. The ultrasound fixation device for extracting an antigen according to claim 1, characterized by: A plurality of positioning grooves are sequentially arranged on the rotating plate.

3. An ultrasonic fixation device for extracting an antigen according to claim 2, characterized in that: The upper end of the ice box is provided with pin shafts at four corner positions, two rubber bands are used to connect the opposite two pin shafts of the ice box respectively, and the beaker is located at the intersection center of the rubber bands.

4. An ultrasound fixation device for extracting an antigen according to claim 3, characterized in that: The lower end of the pin shaft is conical, and the upper end of the pin shaft is provided with an annular groove for embedding the rubber band.

5. An ultrasonic fixation device for extracting an antigen according to claim 4, characterized in that: The lower end of the pin shaft penetrates through the sliding groove, and the first moving plate and the second moving plate are blocked and positioned.

6. An ultrasonic fixation device for extracting an antigen according to claim 5, characterized in that: A plurality of arc-shaped grooves with different diameters are arranged on the first moving plate and the second moving plate.

7. The ultrasound fixation device for extracting an antigen according to claim 1, characterized by: The inner side of the arc-shaped groove is provided with a buffer gasket.

8. An ultrasonic fixation device for extracting an antigen according to claim 7, characterized in that: Magnets are embedded in the end faces of the arc-shaped grooves.

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