A sampling mechanism for a cell mill

Abstract

The utility model discloses a sampling mechanism for cell pulverizer relates to cell technical field, including the rotation plate, and the top rotation link of rotation plate has the extension of rear side of the shaft, and the positive side of the shaft is fixedly connected with the clamping block, and the outer wall of clamping block is connected with the clamping plate, and the bottom of clamping plate is equipped with the boss column and is fixedly connected in the front side of rotation plate, and the bottom rotation link of rotation plate is connected with the connecting rod, and the bottom of connecting rod is hinged with piston axle, and the bottom surface of piston axle is fixedly connected with the piston, and the outside of piston is equipped with the sampling cylinder, and piston slidingly connects in the inner wall of sampling cylinder, and the bottom right side of sampling cylinder is equipped with the liquid outlet hole, and the bottom axle of sampling cylinder is fixedly connected with the suction pipe. The utility model discloses through the rotation handle, drives the disc rotation, and the extension rod rotates, and the clamping plate rotates simultaneously, and when contacting the boss column, utilizes the boss column to drive the rotation plate rotation, and the piston axle is together with piston and slides in the inner wall of sampling cylinder, utilizes the pressure difference, makes the cell in the cell -crushing liquid container inhale to the sampling cylinder, plays the sampling effect.

Description

Technical Field

[0001] This utility model relates to the field of cell technology, specifically to a sampling mechanism for a cell disruptor. Background Technology

[0002] In the field of cell research, cell disruptors are crucial equipment for obtaining intracellular substances. Current cell disruptors have certain shortcomings in the sampling process. Many automated sampling devices are complex in structure, expensive, difficult to maintain, and lack the flexibility to adjust the sampling position and method according to actual needs. While manual sampling is cheaper, traditional manual operation is not convenient or precise enough, especially after sampling, as it is difficult to quickly and easily remove the sampling container, easily leading to sample contamination or spillage, affecting the accuracy and efficiency of the experiment. Therefore, it is necessary to develop a manually controlled cell disruptor sampling device that allows for easy removal of the sampling container after sampling.

[0003] Patent publication number CN217211657U discloses a sampling device for a cell disruptor, including a container cup. A first magnetic ring is fixedly connected to the outside of the container cup. A sampling mechanism is provided inside the container cup. The sampling mechanism consists of a second magnetic ring, an insertion tube, a rubber stopper, a suction tube, a handle, a push rod, and a connector. The second magnetic ring is fixedly connected to the outside of the suction tube. A rubber stopper is movably connected to the inner wall of the suction tube. A push rod is fixedly connected to the upper end face of the rubber stopper. A handle is fixedly connected to the upper end face of the push rod. The connector is connected to the lower end face of the suction tube.

[0004] To address the inconvenience of sampling in cell disruptors, existing technologies employ a method that uses a first and a second magnetic ring to magnetically connect the sampling mechanism to the collection cup. However, this method still suffers from issues such as difficulty in retrieving samples after collection and easy damage to the sampling device, thereby reducing its practicality. Utility Model Content

[0005] To solve the above problems, this utility model provides a sampling mechanism for a cell disruptor, which is achieved through the following technical solution.

[0006] A sampling mechanism for a cell disruptor includes a rotating plate, a rotating shaft rotatably connected to the top of the rotating plate and extending to the rear, a locking block fixed to the front of the rotating shaft, a locking plate locked to the outer wall of the locking block, a protruding post at the bottom of the locking plate and fixed to the front of the rotating plate, a connecting rod rotatably connected to the bottom of the rotating plate, a piston shaft hinged to the bottom of the connecting rod, a piston fixed to the bottom surface of the piston shaft, a sampling cylinder outside the piston, the piston slidably connected to the inner wall of the sampling cylinder, a liquid outlet hole at the bottom right side of the sampling cylinder, and a suction tube fixed to the bottom axis of the sampling cylinder.

[0007] It also includes a collection mechanism, a fixing mechanism, and a driving mechanism. The collection mechanism is installed on the side of the sampling tube to collect the sampled cell fluid. The fixing mechanism is installed on the outer wall of the sampling tube to fix the sampling mechanism. The driving mechanism is installed on the back of the fixing mechanism to drive the card block to rotate.

[0008] As a further embodiment of this utility model, the collection mechanism includes a valve, a collection pipe is fixedly connected to the bottom right side of the sampling cylinder, the collection pipe is connected to the liquid outlet, and the valve is located inside the collection pipe.

[0009] As a further embodiment of this utility model, the collecting mechanism also includes a clamping rod, a sleeve is fixedly connected to the outer right side of the collecting pipe, a through groove is provided on the side of the sleeve, the clamping rod is slidably connected to the inner wall of the through groove, the bottom of the clamping rod is triangular, and a spring is provided on the outer bottom wall of the clamping rod.

[0010] As a further embodiment of this utility model, the collecting mechanism further includes a chuck, a collecting cylinder is inserted into the inner wall of the collecting pipe, the chuck is fixed to the middle outer wall of the collecting cylinder, the outer wall of the chuck is provided with a triangular groove, and the spring is engaged with the chuck.

[0011] As a further embodiment of this utility model, the fixing mechanism includes a fixing sleeve, which is fixedly connected to the outer wall of the sampling cylinder. A fixing plate is fixedly connected to the back of the fixing sleeve, and a pulverizing liquid container is fixedly connected to the back of the fixing plate.

[0012] As a further embodiment of this utility model, the driving mechanism includes a handle, a rotating shaft extending from the top of the rotating plate is fixedly connected to an extension rod, the extension rod is rotatably connected to the inner wall of the fixed sleeve, a disc is fixedly connected to the side of the extension rod away from the fixed sleeve, and the handle is fixedly connected to the side of the disc away from the extension rod.

[0013] The beneficial effects of this utility model are as follows:

[0014] 1. Turn the handle to rotate the disc, the extension rod, and the clamping plate. When the clamping plate contacts the protrusion, it drives the rotating plate to rotate. The piston shaft and piston slide on the inner wall of the sampling cylinder. Using the pressure difference, the cells in the pulverized liquid container are drawn into the sampling cylinder, thus achieving the sampling function.

[0015] 2. After the required cell solution enters the sampling tube, stop turning the handle, insert the collection tube into the inner wall of the collection pipe, and use the spring force to lock the bottom of the clamping rod into the clamping plate. Open the valve, and the cell solution enters into the collection tube for collection, which improves adaptability.

[0016] 3. By using a fixed sleeve to fix the outer wall of the sampling cylinder, the sampling mechanism and the collection mechanism are fixed to the fixed plate. The fixed plate is fixed to the inner wall of the pulverized liquid container, which facilitates sampling and improves adaptability. Attached Figure Description

[0017] To more clearly illustrate the technical solution of this utility model, the drawings used in the description of the specific embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0019] Figure 2 This is a schematic diagram of the sampling mechanism of this utility model;

[0020] Figure 3 This is a schematic diagram of the collection mechanism of this utility model;

[0021] Figure 4 This is a schematic diagram of the fixing mechanism of this utility model;

[0022] Figure 5 This is a schematic diagram of the drive mechanism of this utility model.

[0023] The attached figures are labeled as follows:

[0024] 1. Sampling mechanism; 11. Rotating plate; 12. Clamping block; 13. Clamping plate; 14. Protruding post; 15. Connecting rod; 16. Piston shaft; 17. Piston; 18. Sampling cylinder; 19. Suction tube;

[0025] 2. Collection mechanism; 21. Collection pipe; 22. Valve; 23. Sleeve; 24. Clamping rod; 25. Spring; 26. Chuck; 27. Collection cylinder;

[0026] 3. Fixing mechanism; 31. Fixing sleeve; 32. Fixing plate; 33. Pulverizing liquid container;

[0027] 4. Drive mechanism; 41. Extension rod; 42. Disc; 43. Handle. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0029] like Figure 1-5 As shown, this utility model has the following three specific embodiments.

[0030] Example 1

[0031] A sampling mechanism for a cell disruptor includes a rotating plate 11. A rotating shaft is rotatably connected to the top of the rotating plate 11 and extends to the rear. A locking block 12 is fixed to the front of the rotating shaft. A locking plate 13 is locked to the outer wall of the locking block 12. A protruding post 14 is provided at the bottom of the locking plate 13 and is fixed to the front side of the rotating plate 11. A connecting rod 15 is rotatably connected to the bottom of the rotating plate 11. A piston shaft 16 is hinged to the bottom of the connecting rod 15. A piston 17 is fixed to the bottom surface of the piston shaft 16. A sampling cylinder 18 is provided outside the piston 17. The piston 17 is slidably connected to the inner wall of the sampling cylinder 18. A liquid outlet is provided on the right side of the bottom of the sampling cylinder 18. An aspiration tube 19 is fixed to the bottom axis of the sampling cylinder 18.

[0032] It also includes a collection mechanism 2, a fixing mechanism 3, and a driving mechanism 4. The collection mechanism 2 is installed on the side of the sampling tube 18 and is used to collect the sampled cell fluid. The fixing mechanism 3 is installed on the outer wall of the sampling tube 18 and is used to fix the sampling mechanism 1. The driving mechanism 4 is installed on the back of the fixing mechanism 3 and is used to drive the card block 12 to rotate.

[0033] The drive mechanism 4 includes a handle 43, a rotating shaft extending from the top of the rotating plate 11 and a fixed extension rod 41, the extension rod 41 being rotatably connected to the inner wall of the fixed sleeve 31, a disc 42 being fixedly connected to the side of the extension rod 41 away from the fixed sleeve 31, and the handle 43 being fixedly connected to the side of the disc 42 away from the extension rod 41.

[0034] In this embodiment, as Figure 2 and Figure 5 As shown, rotating the handle 43 causes the disc 42 to rotate, the extension rod 41 to rotate, and the clamping plate 13 to rotate. When it contacts the protrusion 14, the push plate on the outer wall of the clamping plate 13 pushes the protrusion 14, causing the rotating plate 11 to rotate. The piston shaft 16, together with the piston 17, slides on the inner wall of the sampling cylinder 18. By utilizing the pressure difference, the cells in the pulverized liquid container 33 are drawn into the sampling cylinder 18, thus achieving the sampling function.

[0035] Example 2

[0036] The difference from Embodiment 1 is that this embodiment discloses a collection mechanism 2:

[0037] The collection mechanism 2 includes a valve 22. A collection pipe 21 is fixedly connected to the bottom right side of the sampling cylinder 18. The collection pipe 21 is connected to the liquid outlet. The valve 22 is located inside the collection pipe 21.

[0038] Preferably, the collecting mechanism 2 further includes a clamping rod 24, a sleeve 23 is fixedly connected to the outer right side of the collecting pipe 21, a through groove is provided on the side of the sleeve 23, the clamping rod 24 is slidably connected to the inner wall of the through groove, the bottom of the clamping rod 24 is triangular, and a spring 25 is provided on the outer bottom wall of the clamping rod 24.

[0039] Preferably, the collecting mechanism 2 further includes a chuck 26, a collecting cylinder 27 is inserted into the inner wall of the collecting pipe 21, the chuck 26 is fixed to the middle outer wall of the collecting cylinder 27, the outer wall of the chuck 26 is provided with a triangular groove, and the spring 25 is engaged with the chuck 26.

[0040] In this embodiment, as Figure 3 As shown, after the cell fluid enters the sampling tube 18 to the required sample volume, stop turning the handle 43, insert the collection tube 27 into the inner wall of the collection pipe 21, and use the elastic force of the spring 25 to make the bottom of the clamp 24 engage and fix with the chuck 26. Open the valve 22, and the cell fluid enters into the collection tube 27 for collection. When taking out the collection tube 27, pull the clamp 24 upward to drive the spring 25 to retract, and take out the collection tube 27, which improves adaptability.

[0041] Example 3

[0042] The difference between this embodiment and Embodiment 2 is that this embodiment discloses a fixing mechanism:

[0043] The fixing mechanism 3 includes a fixing sleeve 31, which is fixed to the outer wall of the sampling cylinder 18. A fixing plate 32 is fixed to the back of the fixing sleeve 31, and a pulverizing liquid container 33 is fixed to the back of the fixing plate 32.

[0044] In this embodiment, as Figure 4 As shown, by using the fixing sleeve 31 to fix the outer wall of the sampling cylinder 18, the sampling mechanism 1 together with the collection mechanism 2 is fixed on the fixing plate 32. The fixing plate 32 is fixed on the inner wall of the pulverized liquid container 33, which facilitates sampling and improves adaptability.

[0045] The working principle of this utility model is as follows:

[0046] The sampling cylinder 18 is fixed to the outer wall using the fixed sleeve 31, and the sampling mechanism 1 and the collection mechanism 2 are fixed to the fixed plate 32. The fixed plate 32 is fixed to the inner wall of the pulverized liquid container 33. Turning the handle 43 causes the disc 42 to rotate, the extension rod 41 to rotate, and the clamping plate 13 to rotate. When it contacts the protrusion 14, the push plate on the outer wall of the clamping plate 13 pushes the protrusion 14, causing the rotating plate 11 to rotate. The piston shaft 16 and the piston 17 slide on the inner wall of the sampling cylinder 18, using pressure. The strong difference causes the cells in the pulverized liquid container 33 to be drawn into the sampling tube 18. After the cell fluid enters the sampling tube 18 to the required sample volume, stop turning the handle 43, insert the collection tube 27 into the inner wall of the collection pipe 21, and use the elastic force of the spring 25 to make the bottom of the clamp 24 engage and fix with the chuck 26. Open the valve 22, and the cell fluid enters the collection tube 27 for collection. When taking out the collection tube 27, pull the clamp 24 upward to drive the spring 25 to retract and take out the collection tube 27.

[0047] The present invention has been described in detail above. However, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, any modifications or improvements that do not depart from the spirit of the present invention are within the protection scope of the present invention.

Claims

1. A sampling mechanism for a cell disruptor, comprising a rotating plate (11), characterized in that: The top of the rotating plate (11) is rotatably connected to a rotating shaft and extends to the rear. A locking block (12) is fixedly connected to the front of the rotating shaft. A locking plate (13) is locked to the outer wall of the locking block (12). A protruding post (14) is provided at the bottom of the locking plate (13) and is fixed to the front side of the rotating plate (11). A connecting rod (15) is rotatably connected to the bottom of the rotating plate (11). A piston shaft (16) is hinged to the bottom of the connecting rod (15). A piston (17) is fixed to the bottom surface of the piston shaft (16). A sampling cylinder (18) is provided outside the piston (17). The piston (17) is slidably connected to the inner wall of the sampling cylinder (18). A liquid outlet hole is provided on the right side of the bottom of the sampling cylinder (18). A suction tube (19) is fixed to the bottom axis of the sampling cylinder (18). It also includes a collection mechanism (2), a fixing mechanism (3) and a driving mechanism (4). The collection mechanism (2) is installed on the side of the sampling tube (18) for collecting the sampled cell fluid. The fixing mechanism (3) is installed on the outer wall of the sampling tube (18) for fixing the sampling mechanism (1). The driving mechanism (4) is installed on the back of the fixing mechanism (3) for driving the card block (12) to rotate.

2. The sampling mechanism for a cell disruptor according to claim 1, characterized in that: The collection mechanism (2) includes a valve (22), and a collection pipe (21) is fixedly connected to the bottom right side of the sampling tube (18). The collection pipe (21) is connected to the liquid outlet, and the valve (22) is located inside the collection pipe (21).

3. A sampling mechanism for a cell disruptor according to claim 2, characterized in that: The collecting mechanism (2) also includes a clamping rod (24). A sleeve (23) is fixed to the outer right side of the collecting pipe (21). A through groove is provided on the side of the sleeve (23). The clamping rod (24) is slidably connected to the inner wall of the through groove. The bottom of the clamping rod (24) is triangular. A spring (25) is provided on the outer bottom wall of the clamping rod (24).

4. A sampling mechanism for a cell disruptor according to claim 3, characterized in that: The collecting mechanism (2) also includes a chuck (26), a collecting cylinder (27) is inserted into the inner wall of the collecting pipe (21), the chuck (26) is fixed to the middle outer wall of the collecting cylinder (27), the outer wall of the chuck (26) is provided with a triangular groove, and the spring (25) is engaged with the chuck (26).

5. A sampling mechanism for a cell disruptor according to claim 1, characterized in that: The fixing mechanism (3) includes a fixing sleeve (31), which is fixed to the outer wall of the sampling tube (18). A fixing plate (32) is fixed to the back of the fixing sleeve (31), and a pulverizing liquid container (33) is fixed to the back of the fixing plate (32).

6. A sampling mechanism for a cell disruptor according to claim 1, characterized in that: The drive mechanism (4) includes a handle (43), a rotating shaft extending from the top of the rotating plate (11) is fixedly connected to an extension rod (41), the extension rod (41) is rotatably connected to the inner wall of the fixed sleeve (31), a disc (42) is fixedly connected to the side of the extension rod (41) away from the fixed sleeve (31), and the handle (43) is fixedly connected to the side of the disc (42) away from the extension rod (41).

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