A device for phage ligation reactions

Abstract

The application provides a bacteriophage ligation reaction device, and belongs to the technical field of ligation reaction. The bacteriophage ligation reaction device comprises a driving assembly and a reaction assembly. The driving assembly comprises a support plate, a shell, a motor and an inlet and outlet water pipe. The shell is rotationally arranged in the support plate. The reaction assembly comprises an inner container, a connecting piece, a cover plate and a thermometer. The inner container is arranged in the shell, and a sandwich layer is formed between the shell and the inner container. By arranging the support plate, the shell, the motor and the inlet and outlet water pipe, the shell is driven by the motor to be adjusted or rotated at an angle, so that the unloading is facilitated. By arranging the inner container, the connecting piece, the cover plate and the thermometer, the shell and the inner container can be conveniently disassembled, so that the inner wall of the shell and the outer wall of the inner container can be conveniently cleaned, and meanwhile, the shell can be rotated and inclined, so that the disassembly is facilitated at a suitable angle.

Description

Technical Field

[0001] This application relates to the field of ligation reactions, and more specifically, to a device for phage ligation reactions. Background Technology

[0002] Ligation is a biochemical process involving the formation of phosphodiester bonds via enzyme catalysis, both in vivo and in vitro, and is primarily used for the construction of recombinant DNA. T4 phage DNA ligase and E. coli DNA ligase are commonly used enzymes, requiring a buffer system containing Mg²⁺ and ATP at 12-16°C to maintain end stability. The reaction mechanism involves three stages: enzymatic adenylation, adenosine group transfer, and phosphodiester bond formation. In laboratory applications, the DNA concentration should be controlled at 5-10 μg / ml, and the molar ratio of vector to insert fragment should be 1:1 to 1:10.

[0003] Currently, phage ligation reactions require constant temperature control to ensure that the temperature remains within a suitable range during the reaction. However, traditional methods of temperature control using water in the tank jacket are prone to scale buildup that is difficult to clean, leading to poor heat exchange and ultimately affecting the phage ligation reaction. Utility Model Content

[0004] To overcome the above deficiencies, this application provides a phage ligation reaction apparatus, which aims to improve the problems mentioned in the background art.

[0005] This application provides a phage linkage reaction apparatus including a driving component and a reaction component.

[0006] The drive assembly includes a support plate, a housing, a motor, and inlet and outlet water pipes. The housing is rotatably disposed within the support plate, the motor is mounted on one side of the support plate, the housing is fixedly connected to a rotating shaft, and the motor is drivenly connected to the rotating shaft.

[0007] The reaction assembly includes an inner liner, a connector, a cover plate, and a thermometer. The inner liner is disposed inside the outer shell, and a sandwich is formed between the outer shell and the inner liner. The connector is disposed between the top of the outer shell and the inner liner. The cover plate is threaded to the inner liner, and the thermometer is fixedly inserted through the cover plate.

[0008] In one specific implementation, the support plate includes a base and an upright plate, the upright plate being vertically fixed to the base, the rotating shaft passing through the base, and the motor being mounted on the side of the upright plate.

[0009] In one specific implementation, a drive gear is fixedly connected to the output end of the motor, and a driven gear is fixedly connected to the rotating shaft, with the drive gear and the driven gear meshing together.

[0010] In the above implementation process, by setting the driving gear and the driven gear to mesh, the motor is started to drive the driving gear to rotate, the driving gear meshes to drive the driven gear to rotate, and the driven gear drives the shaft to rotate, thus realizing the transmission connection.

[0011] In one specific implementation, a sealing ring is provided on the outer side of the inner liner, and a rubber ring is provided on the outer side of the sealing ring and fits against the inner wall of the outer shell.

[0012] In the above implementation process, the sealing performance of the inner liner and the support plate is improved by setting a sealing ring and a rubber ring.

[0013] In one specific implementation, the connector includes a fixing plate, a threaded rod, and a nut. The fixing plate is fixedly connected to the outer shell, the threaded rod is fixedly connected to the inner liner, the threaded rod slides through the fixing plate, and the nut is threadedly sleeved on the threaded rod.

[0014] In the above implementation process, by setting a fixing plate, a threaded rod and a nut, when the inner liner is placed into the outer shell, the threaded rod passes through the fixing plate, and then the nut is tightened to connect and fix it, thus facilitating disassembly and assembly.

[0015] In one specific implementation, both the fixing plate and the threaded rod are chamfered.

[0016] In the above implementation process, a chamfer is made to facilitate the alignment of the threaded rod with the fixing plate during installation.

[0017] In one specific implementation, the inner liner is provided with an arc-shaped protrusion, and the arc-shaped protrusion is connected to the interlayer.

[0018] In the above implementation process, by setting arc-shaped protrusions, the contact area with the internal phage connection reaction solution is increased, thereby enhancing the effect of heat exchange to stabilize the temperature.

[0019] In one specific implementation, the top of the inner liner is provided with an external threaded ring, and the cover plate is threadedly connected to the external threaded ring.

[0020] In the above implementation process, by setting an external threaded ring, the cover plate can be installed or removed by threaded connection.

[0021] Beneficial effects: This application provides a phage linkage reaction device. By setting up a support plate, outer shell, motor and inlet / outlet water pipes, the motor drives the outer shell to adjust the angle or rotate, which facilitates unloading. By setting up an inner liner, connectors, cover plate and thermometer, the outer shell and inner liner can be easily disassembled and assembled, which allows for the cleaning of scale on the inner wall of the outer shell and the outer wall of the inner liner. At the same time, the tiltable outer shell can be rotated, which is conducive to disassembly and assembly at a suitable angle. Attached Figure Description

[0022] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0023] Figure 1 This is a schematic diagram of the structure of the phage linkage reaction device provided in the embodiments of this application; Figure 2 A schematic diagram of the moving component structure provided for an embodiment of this application; Figure 3 A schematic diagram of the inner liner structure provided for an embodiment of this application; Figure 4 A schematic diagram of the cover plate structure provided for an embodiment of this application; Figure 5 A schematic diagram of the sandwich structure provided for an embodiment of this application.

[0024] In the diagram: 100-Drive assembly; 110-Support plate; 111-Base; 112-Upright plate; 130-Outer shell; 131-Rotating shaft; 132-Driven gear; 140-Motor; 141-Drive gear; 160-Inlet / outlet water pipe; 200-Reaction assembly; 210-Inner liner; 211-Sealing ring; 212-Arc-shaped protrusion; 220-Connector; 221-Fixing hole plate; 222-Threaded rod; 223-Nut; 230-Cover plate; 250-Thermometer; 260-Interlayer; 270-External threaded ring. Detailed Implementation

[0025] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments.

[0026] Please see Figures 1-5 This application provides a phage linkage reaction apparatus including a drive assembly 100 and a reaction assembly 200.

[0027] Please see Figure 1 and 2 The drive assembly 100 includes a support plate 110, a housing 130, a motor 140, and an inlet / outlet water pipe 160. The housing 130 is rotatably disposed inside the support plate 110, the motor 140 is installed on one side of the support plate 110, and the housing 130 is fixedly connected to a rotating shaft 131, and the motor 140 is drivenly connected to the rotating shaft 131.

[0028] The support plate 110 includes a base 111 and an upright plate 112. The upright plate 112 is vertically fixed to the base 111. The rotating shaft 131 rotates through the base 111. The motor 140 is installed on the side of the upright plate 112. The output end of the motor 140 is fixedly connected to a drive gear 141. The rotating shaft 131 is fixedly connected to a driven gear 132. The drive gear 141 and the driven gear 132 mesh with each other. By setting the drive gear 141 and the driven gear 132 to mesh, the motor 140 is started to drive the drive gear 141 to rotate. The drive gear 141 meshes with the driven gear 132 to rotate. The driven gear 132 drives the rotating shaft 131 to rotate, thus realizing the transmission connection.

[0029] It should be noted that the external water supply hose is connected through two inlet and outlet water pipes 160, and the circulating water supply is a conventional technology well known to those in the field. The specific principle and structure will not be elaborated on here.

[0030] Please see Figure 1 , 2 3, 4 and 5, the reaction assembly 200 includes an inner liner 210, a connector 220, a cover plate 230 and a thermometer 250. The inner liner 210 is disposed inside the outer shell 130, and a sandwich 260 is formed between the outer shell 130 and the inner liner 210. The connector 220 is disposed between the top of the outer shell 130 and the inner liner 210. The cover plate 230 is threaded to the inner liner 210. The thermometer 250 is fixedly inserted through the cover plate 230.

[0031] The inner liner 210 is provided with a sealing ring 211 on the outside, and a rubber ring is provided on the outside of the sealing ring 211 and fits against the inner wall of the outer shell 130. By providing the sealing ring 211 and the rubber ring, the sealing performance of the inner liner 210 and the support plate 110 after connection is improved.

[0032] Specifically, the connector 220 includes a fixing plate 221, a threaded rod 222, and a nut 223. The fixing plate 221 is fixedly connected to the outer shell 130, and the threaded rod 222 is fixedly connected to the inner liner 210. The threaded rod 222 slides through the fixing plate 221, and the nut 223 is threaded onto the threaded rod 222. By setting the fixing plate 221, the threaded rod 222, and the nut 223, when the inner liner 210 is placed into the outer shell 130, the threaded rod 222 passes through the fixing plate 221, and then the nut 223 is tightened to connect and fix the connector, thus facilitating disassembly and assembly.

[0033] It should be noted that both the fixing plate 221 and the threaded rod 222 are chamfered. The chamfering makes it easier for the threaded rod 222 to be aligned and pass through the fixing plate 221 during installation.

[0034] In this embodiment, the inner liner 210 is provided with an arc-shaped protrusion 212, and the arc-shaped protrusion 212 is connected to the interlayer 260. By providing the arc-shaped protrusion 212, the contact area with the internal phage connection reaction liquid is increased, thereby improving the effect of heat exchange to stabilize the temperature.

[0035] In one specific implementation, the inner liner 210 is provided with an external threaded ring 270 at its top, and the cover plate 230 is threadedly connected to the external threaded ring 270. By providing the external threaded ring 270, the cover plate 230 can be threadedly installed or removed.

[0036] The working principle of this phage linkage reaction device is as follows: During use, circulating water enters from one inlet / outlet pipe 160 and exits from the other. An arc-shaped protrusion 212, connected to the interlayer 260, increases the contact area with the internal phage linkage reaction solution, enhancing heat exchange and stabilizing the temperature. After the phage linkage reaction, the cover plate 230 is removed by rotation. The motor 140 is then started, driving the drive gear 141 to rotate. The drive gear 141 meshes with and drives the driven gear 132 to rotate, which in turn drives the rotating shaft 131 to rotate. Shaft 131 drives the outer casing 130 to rotate and tilt for unloading. When it is necessary to clean scale, motor 140 is started to drive drive gear 141 to rotate. Drive gear 141 meshes and drives driven gear 132 to rotate. Driven gear 132 drives shaft 131 to rotate. Shaft 131 drives outer casing 130 to rotate and tilt. Then, rotate and remove each nut 223 to remove inner liner 210 from outer casing 130. Scale on the inner wall of outer casing 130 and outer wall of inner liner 210 can be cleaned separately. The tiltable outer casing 130 facilitates disassembly and assembly at a suitable angle.

[0037] It should be noted that the specific model and specifications of motor 140 need to be selected and determined based on the actual specifications of the device. The specific selection and calculation method adopts the existing technology in this field, so it will not be described in detail here.

[0038] The power supply and principle of motor 140 are clear to those skilled in the art and will not be described in detail here.

[0039] It will be apparent to those skilled in the art that this application is not limited to the details of the exemplary embodiments described above, and that this application can be implemented in other specific forms without departing from the spirit or essential characteristics of this application. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this application is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this application. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A device for phage ligation reaction, characterized in that, include The drive assembly (100) includes a support plate (110), a housing (130), a motor (140), and inlet / outlet water pipes (160). The housing (130) is rotatably disposed inside the support plate (110). The motor (140) is installed on one side of the support plate (110). The housing (130) is fixedly connected to a rotating shaft (131), and the motor (140) is drivenly connected to the rotating shaft (131). The reaction assembly (200) includes an inner liner (210), a connector (220), a cover plate (230), and a thermometer (250). The inner liner (210) is disposed inside the outer shell (130), and a sandwich layer (260) is formed between the outer shell (130) and the inner liner (210). The connector (220) is disposed between the top of the outer shell (130) and the inner liner (210). The cover plate (230) is threaded to the inner liner (210), and the thermometer (250) is fixedly inserted through the cover plate (230).

2. The phage ligation reaction apparatus according to claim 1, characterized in that, The support plate (110) includes a base (111) and an upright plate (112). The upright plate (112) is vertically fixed to the base (111). The rotating shaft (131) rotates through the base (111). The motor (140) is installed on the side of the upright plate (112).

3. The apparatus for phage ligation reaction according to claim 1, characterized in that, The output end of the motor (140) is fixedly connected to a drive gear (141), and the shaft (131) is fixedly connected to a driven gear (132). The drive gear (141) and the driven gear (132) mesh with each other.

4. The apparatus for phage ligation reaction according to claim 1, characterized in that, A sealing ring (211) is provided on the outside of the inner liner (210), and a rubber ring is provided on the outside of the sealing ring (211) and fits against the inner wall of the outer shell (130).

5. The apparatus for phage ligation reaction according to claim 1, characterized in that, The connector (220) includes a fixing plate (221), a threaded rod (222), and a nut (223). The fixing plate (221) is fixedly connected to the outer shell (130), the threaded rod (222) is fixedly connected to the inner liner (210), the threaded rod (222) slides through the fixing plate (221), and the nut (223) is threadedly sleeved on the threaded rod (222).

6. The apparatus for phage ligation reaction according to claim 5, characterized in that, Both the fixed hole plate (221) and the threaded rod (222) are chamfered.

7. The apparatus for phage ligation reaction according to claim 1, characterized in that, The inner liner (210) has an arc-shaped protrusion (212) inside, and the arc-shaped protrusion (212) is connected to the interlayer (260).

8. The apparatus for phage ligation reaction according to claim 1, characterized in that, The inner liner (210) is provided with an external threaded ring (270) at the top, and the cover plate (230) is threaded to the external threaded ring (270).

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