A special culture dish for microscopic observation of cell drug treatment

Abstract

The utility model discloses a cell medicine handles microscopical observation special culture dish belongs to culture dish technical field. Including dish body and dish cover, dish cover covers the top surface of dish body, the inside installation of dish body has cross bulkhead, and cross bulkhead divides the space in dish body into four groups of culture cavities, and the top surface of dish cover is provided with four groups of through -holes, and four groups of through -holes and four groups of culture cavities correspond one -to -one, and the bottom surface of through -hole all has the through -pipe that communicates, and the inside installation of through -pipe all has rubber plug, and the center of rubber plug is provided with liquid injection hole, this technical scheme divides the space into four groups of independent culture cavities through the cross bulkhead in dish body, and cooperates the through -hole, through -pipe and rubber plug with liquid injection hole of corresponding one -to -one on dish cover, effectively solved the problem that the traditional single cavity culture dish needs to use multiple culture dishes in multiple drug concentration gradient experiments, resulting in the problems of large consumable consumption, poor consistency between groups, complicated operation and high error risk.

Description

Technical Field

[0001] This utility model relates to the field of culture dish technology, specifically a culture dish for microscopic observation of cell drug treatment. Background Technology

[0002] In cell biology research and drug development, observing morphological changes, proliferation capacity, and physiological activity responses of cells after treatment with different concentrations of drugs under a microscope is a core experimental method for analyzing drug mechanisms of action and screening effective doses. As the basic carrier for in vitro cell culture, the structural design of the culture dish directly affects the convenience of experimental operations, the reliability of data, and the reproducibility of results.

[0003] Currently, conventional culture dishes are mostly single-cavity structures, which have significant limitations when conducting multiple drug concentration gradient experiments. Because different drug concentrations cannot be independently processed within the same dish, researchers need to prepare multiple dishes, each separately seeded with cells and the corresponding drug concentration added. This approach not only increases the consumption of consumables such as culture dishes, culture media, and drugs, but also makes it difficult to ensure absolute consistency in initial cell seeding density and culture environment, leading to increased inter-group errors, as multiple experiments must be conducted in different dishes. Furthermore, the handling, drug addition, and observation of multiple culture dishes are cumbersome, especially in large-scale drug screening experiments, where operator fatigue can easily introduce human error, affecting the accuracy of experimental results. Therefore, developing a dedicated culture dish capable of conducting multiple drug concentration gradient experiments within a single dish is of great significance for improving experimental efficiency and reducing the risk of error. Utility Model Content

[0004] The purpose of this invention is to provide a special culture dish for cell drug treatment and microscopic observation, so as to solve the problems mentioned in the background art.

[0005] In view of the above problems, the technical solution proposed by this utility model is as follows:

[0006] A cell drug treatment microscopic observation culture dish includes a dish body and a lid. The lid covers the top surface of the dish body. A cross-shaped partition is installed inside the dish body, dividing the internal space of the dish body into four equal groups of culture chambers. The cross-shaped partition divides the dish body into four independent culture chambers, allowing for simultaneous performance of multiple drug concentration gradient experiments, reducing consumable usage, ensuring consistency of experimental conditions across groups, and minimizing inter-group errors. The top surface of the lid has four sets of through holes, each corresponding to one of the four culture chambers. The bottom surface of each through hole is connected to a connecting tube, and each connecting tube has a rubber stopper installed inside. The center of each rubber stopper has an injection hole, which corresponds to one of the culture chambers. With the help of the connecting tube and the injection hole of the rubber stopper, drugs can be precisely added to specific culture chambers, avoiding cross-contamination. Furthermore, the dish body does not need to be opened, reducing interference from the external environment on the cells.

[0007] Furthermore, a connecting ring is installed on the top surface of the dish body, which fits snugly against the inner wall of the dish lid, enhancing the sealing between the dish body and the dish lid, reducing the evaporation of the culture medium in the culture chamber, and preventing external contaminants from entering, thus ensuring a stable cell culture environment.

[0008] Furthermore, a sealing ring is installed on the outer side of the connecting ring. The sealing ring further enhances the sealing between the connecting ring and the dish lid, effectively preventing external dust and microorganisms from entering the culture chamber, while minimizing the evaporation of the culture medium and extending the cell survival time.

[0009] Furthermore, a pair of connecting blocks are installed on the side of the dish lid, and a rotating shaft is connected between the pair of connecting blocks by a torsion spring. A flip cover is installed on the outside of the rotating shaft, and the flip cover is in contact with the top surface of the dish lid. The flip cover can cover the through hole on the top surface of the dish lid to prevent dust and other impurities from falling into the through hole and contaminating the rubber stopper. At the same time, it reduces the impact of direct light on photosensitive cells. The torsion spring enables the flip cover to have an automatic reset function. It can automatically pop open after being opened, making it convenient to operate without manual fixing.

[0010] Furthermore, an iron sheet is installed on the side of the flip cover, and a fixing block is installed on the side of the petri dish lid. A magnet is embedded on the top surface of the fixing block. The magnet attracts the iron sheet, which can firmly fix the flip cover in the closed state, preventing the flip cover from being accidentally opened due to vibration or flipping of the petri dish, and ensuring the cleanliness of the through holes and the stability of the culture environment.

[0011] Furthermore, a marking groove is provided on the bottom surface of the culture chamber. The diameter of the marking groove is 0.5 mm and the depth of the marking groove is less than 0.01 mm. The marking groove can be used as a positioning mark during microscope observation, which facilitates the quick finding of the same field of view for long-term tracking observation. It is especially suitable for recording the dynamic changes of cells after drug treatment. The groove is small and does not affect cell growth or interfere with the clarity of microscope imaging.

[0012] Furthermore, the dish body is made of transparent plastic, which ensures good light transmittance and does not affect the observation of cells inside the culture chamber by the microscope, allowing the cell morphology and physiological state to be clearly presented.

[0013] Compared with existing technologies, the beneficial effects of this invention are as follows: This specialized culture dish for cell drug treatment microscopic observation divides the space into four independent culture chambers through a cross-shaped partition within the dish. Combined with corresponding through holes, tubes, and rubber stoppers with injection holes on the dish lid, it effectively solves the problems of high consumable consumption, poor inter-group environmental consistency, cumbersome operation, and high error risk associated with traditional single-chamber culture dishes used in multi-group drug concentration gradient experiments. Four sets of experiments can be conducted simultaneously within a single dish, ensuring consistent initial cell density and culture environment. The independent injection structure avoids cross-contamination, reduces operational steps, significantly reduces inter-group errors and human error, and improves experimental efficiency and result reliability. Attached Figure Description

[0014] Figure 1 This is a three-dimensional structural schematic diagram of the cell drug treatment microscopic observation culture dish disclosed in the embodiments of this utility model;

[0015] Figure 2 This is a schematic diagram of the exploded structure of a special culture dish for microscopic observation of cell drug treatment disclosed in an embodiment of this utility model;

[0016] Figure 3 for Figure 2 Enlarged schematic diagram of structure A in the middle;

[0017] Figure 4 This is a schematic cross-sectional view of the culture dish for microscopic observation of cell drug treatment disclosed in this embodiment of the present invention.

[0018] In the diagram: 1. Dish body; 2. Dish lid; 3. Connecting block; 4. Flip lid; 5. Iron sheet; 6. Fixing block; 7. Magnet; 8. Through hole; 9. Cross partition; 10. Culture chamber; 11. Marking groove; 12. Connecting ring; 13. Rubber stopper; 14. Injection hole; 15. Through tube. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Please see Figure 1 - Figure 4This utility model provides a technical solution: a special culture dish for microscopic observation of cell drug treatment, including a dish body 1 and a dish lid 2. The dish lid 2 covers the top surface of the dish body 1. A cross partition 9 is installed inside the dish body 1, which divides the space inside the dish body 1 into four groups of culture chambers 10. The top surface of the dish lid 2 has four sets of through holes 8, and the four sets of through holes 8 correspond one-to-one with the four groups of culture chambers 10. The bottom surface of each through hole 8 is connected to a through tube 15. A rubber stopper 13 is installed inside each through tube 15. An injection hole 14 is opened at the center of the rubber stopper 13. The four groups of culture chambers 10 can be inoculated with cells of the same density. Through the through tube 15 below the corresponding through hole 8, the pipette needle is passed through the injection hole 14 of the rubber stopper 13 to add different concentrations of drugs to different culture chambers 10, realizing multiple parallel experiments in a single dish. The rubber stopper 13 is elastic, and the injection hole 14 can automatically close after injection to maintain the closed environment of the culture chamber 10 and prevent contamination by bacteria and evaporation of culture medium.

[0021] As an embodiment of this utility model, a connector 12 is further installed on the top surface of the dish body 1. The connector 12 fits against the inner wall of the dish cover 2. When the dish cover 2 is closed, the connector 12 is embedded in the inner wall of the dish cover 2 to form a tightly fitted structure, reducing the gap between the two, reducing the exchange of gas and liquid, and maintaining the microenvironment in the culture chamber 10.

[0022] As an embodiment of this utility model, a sealing ring is further installed on the outer side of the connecting ring 12. The sealing ring is made of elastic material. When the lid 2 is closed, the sealing ring is squeezed and deformed, filling the tiny gaps between the connecting ring 12 and the inner wall of the lid 2, forming a tighter sealing barrier and improving the overall sealing performance.

[0023] As an embodiment of this utility model, a pair of connecting blocks 3 are further installed on the side of the dish lid 2. A rotating shaft is connected between the pair of connecting blocks 3 by a torsion spring. A flip cover 4 is installed on the outside of the rotating shaft, and the flip cover 4 is in contact with the top surface of the dish lid 2. The flip cover 4 is connected to the connecting blocks 3 through the rotating shaft. The torsion spring causes the flip cover 4 to spring open in its natural state. When liquid needs to be injected, the flip cover 4 is lifted.

[0024] As an embodiment of this utility model, the side of the flip cover 4 is further provided with an iron sheet 5, and the side of the lid 2 is provided with a fixing block 6. The top surface of the fixing block 6 is embedded with a magnet 7. The magnet 7 attracts the iron sheet 5. When the flip cover 4 is closed, the iron sheet 5 contacts and is attracted to the magnet 7 on the fixing block 6, and the position of the flip cover 4 is fixed by magnetic force. When it is necessary to open, a slight external force is applied to make the iron sheet 5 disengage from the magnet 7, and the flip cover 4 remains open under the action of the torsion spring.

[0025] As an embodiment of this utility model, the bottom surface of the culture chamber 10 is provided with a marking groove 11. The diameter of the marking groove 11 is 0.5 mm and the depth of the marking groove 11 is less than 0.01 mm. When observed under a microscope, the marking groove 11 forms a light-dark contrast with the surrounding area, which can be used as a reference point to mark the position of a specific cell group, making it convenient to quickly locate the cell group during subsequent observation and reducing observation errors caused by field of view shift.

[0026] As an embodiment of this utility model, the dish body 1 is further made of transparent plastic, which allows light to pass through. The light source of the microscope can illuminate the cells through the dish body 1, so that the eyepiece or imaging system can clearly capture the cell image and meet the basic requirements of microscopic observation.

[0027] It should be noted that all standard parts used in this application can be purchased from the market, and can be customized according to the description and drawings. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts and equipment adopt conventional models in the prior art.

Claims

1. A special culture dish for cell drug treatment and microscopic observation, characterized in that, The device includes a dish body (1) and a dish lid (2). The dish lid (2) covers the top surface of the dish body (1). A cross partition (9) is installed inside the dish body (1). The cross partition (9) divides the space inside the dish body (1) into four groups of culture chambers (10). The top surface of the dish lid (2) has four groups of through holes (8), and the four groups of through holes (8) correspond one-to-one with the four groups of culture chambers (10). The bottom surface of each through hole (8) is connected to a through tube (15). A rubber stopper (13) is installed inside each through tube (15). An injection hole (14) is opened at the center of the rubber stopper (13).

2. The cell drug treatment microscopic observation culture dish according to claim 1, characterized in that, A connecting ring (12) is installed on the top surface of the dish body (1), and the connecting ring (12) is in contact with the inner wall of the dish lid (2).

3. The cell drug treatment microscopic observation culture dish according to claim 2, characterized in that, A sealing ring is installed on the outside of the connecting ring (12).

4. The cell drug treatment microscopic observation culture dish according to claim 1, characterized in that, A pair of connecting blocks (3) are installed on the side of the lid (2). A rotating shaft is connected between the pair of connecting blocks (3) by a torsion spring. A flip cover (4) is installed on the outside of the rotating shaft, and the flip cover (4) and the top surface of the lid (2) are in contact.

5. The cell drug treatment microscopic observation culture dish according to claim 4, characterized in that, The flip cover (4) has an iron sheet (5) installed on its side, and the lid (2) has a fixing block (6) installed on its side. The top surface of the fixing block (6) is embedded with a magnet (7), which attracts the iron sheet (5).

6. The cell drug treatment microscopic observation culture dish according to claim 1, characterized in that, The bottom surface of the culture chamber (10) is provided with a marking groove (11), the diameter of the marking groove (11) is 0.5 mm, and the depth of the marking groove (11) is less than 0.01 mm.

7. The cell drug treatment microscopic observation culture dish according to claim 1, characterized in that, The dish body (1) is made of transparent plastic.

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