Plant exosome extraction and purification machine

Abstract

The utility model relates to plant extraction technical field discloses a kind of plant exosome extraction and purification machine, including bottom plate, the left side fixedly connected with bracket of bottom plate, the output end fixedly connected with multiple blades of motor one, the outer wall of wall breaking box is connected with filter box, the inner wall fixedly connected with multiple activated carbon plate of filter box, the bottom of filter box is connected with burette, the outer wall fixedly connected with piston of burette, the top fixedly connected with fixed mechanism of bottom plate, the fixed mechanism is used for fast installation.In the utility model, open motor one, blade breaks the wall of plant, activated carbon plate filters out residue and pigment, piston adjusts the flow rate and dosage of juice, let juice drop into mixed test tube, simplify the operation of early separation filtering of plant solvent, reduce the time cost of experiment, improve the efficiency of extraction and purification, increase practicality, expand the scope of application.

Description

Technical Field

[0001] This utility model relates to the field of plant extraction technology, and in particular to a plant exosome extraction and purification machine. Background Technology

[0002] Plant exosomes, as nanoscale lipid bilayer vesicles secreted by plant cells, have shown great potential in various fields such as anti-inflammation, anti-oxidation, disease treatment, and drug delivery due to carrying bioactive molecules such as proteins, nucleic acids, and lipids. With the continuous development of science and technology, emerging technologies can significantly improve extraction efficiency and preserve the integrity of exosomes, while supporting large-scale production. Plant exosome technology is transitioning from laboratory research to industrialization. Its natural targeting, biocompatibility, and potential for large-scale production have driven its application exploration in multiple fields such as precision medicine and green biomanufacturing. Combined with intelligent control and novel membrane materials, plant exosome extraction and purification technology is expected to achieve breakthrough applications in the pharmaceutical and agricultural fields.

[0003] Plant exosomes are widely used in medicine, agriculture, and food, showing great potential in anti-inflammatory, antioxidant, disease treatment, and drug delivery fields. However, existing plant exosome extraction and purification machines cannot directly extract the desired substances. They require separate cell wall disruption of the plant, followed by filtration and separation of the various substances after cell wall disruption. This process is cumbersome and complex, increases experimental time costs, reduces extraction and purification efficiency, diminishes practicality, and narrows the scope of application. Utility Model Content

[0004] To overcome the above shortcomings, this utility model provides a plant exosome extraction and purification machine, which aims to improve the problems of complex pretreatment and difficult assembly and disassembly in the existing technology.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a plant exosome extraction and purification machine, comprising a base plate, a support fixedly connected to the left side of the base plate, a motor fixedly connected to the inner wall of the support, a pectinase solution pool fixedly connected to the inner wall of the support, a PBS solution pool fixedly connected to the inner wall of the support, multiple blades fixedly connected to the output end of the motor, a wall-breaking chamber fixedly connected to the top of the support, a filter box connected to the outer wall of the wall-breaking chamber, multiple activated carbon plates fixedly connected to the inner wall of the filter box, a burette connected to the bottom of the filter box, a piston fixedly connected to the outer wall of the burette, a guide rail fixedly connected to the outer wall of the support, a slider slidably connected to the top of the guide rail, and a fixing mechanism fixedly connected to the top of the base plate for quick installation.

[0006] As a further description of the above technical solution:

[0007] The fixing mechanism includes a support frame, the top of which is fixedly connected to the bottom of a base plate. A second motor is fixedly connected to the top of the support frame, and a turntable is fixedly connected to the output end of the second motor. The inner wall of the turntable has multiple displacement grooves, and columns are slidably connected to the inner walls of the multiple displacement grooves. Pressure blocks are fixedly connected to the bottom of the multiple columns. A base is slidably connected to the bottom of the turntable, and a sliding groove is formed on the top of the base. A shell is rotatably connected to the outer wall of the base. A rotor is slidably connected to the outer wall of the multiple pressure blocks, and multiple test tube grooves are formed on the top of the rotor.

[0008] As a further description of the above technical solution:

[0009] The outer wall of the blender is fixedly connected to a hinge, and an end cap is fixedly connected to the right side of the hinge.

[0010] As a further description of the above technical solution:

[0011] The inner wall of the bracket is fixedly connected to a protective cover, and the outer wall of the protective cover has multiple ventilation holes.

[0012] As a further description of the above technical solution:

[0013] The bottom of the base plate is fixedly connected to multiple support columns, and the top of the base plate is fixedly connected to a control box.

[0014] As a further description of the above technical solution:

[0015] The bottom of each of the multiple support columns is fixedly connected to a chassis, and the bottom of each of the multiple chassis is fixedly connected to an anti-slip pad.

[0016] As a further description of the above technical solution:

[0017] A nameplate is fixedly connected to the left side of the bracket, and a mixing test tube is slidably connected to the inner wall of the slider.

[0018] As a further description of the above technical solution:

[0019] The bottom of the guide rail is fixedly connected to the top of the base plate, and the outer wall of the filter box is fixedly connected to the inner wall of the bracket.

[0020] This utility model has the following beneficial effects:

[0021] 1. In this utility model, when the motor is turned on, the blade rotates at high speed to break the plant cell walls. After multi-stage filtration by the activated carbon plate, the residue and pigments in the juice are filtered out and flow into the burette. By controlling the piston to adjust the flow rate and dosage of the juice, the juice is dripped into the mixing tube. Then, a certain amount of PBS solution and pectinase solution are added to mix them thoroughly. This simplifies the initial separation and filtration of plant solvents, reduces experimental time costs, improves extraction and purification efficiency, increases practicality, and expands the scope of application.

[0022] 2. In this utility model, the rotation of motor two drives the turntable to rotate, the displacement groove cooperates with the column to make the column move horizontally, and the pressure block also moves horizontally and is stuck at the bottom of the rotor. Reversing motor two releases the rotor, which can be quickly disassembled when there is a problem with the rotor, making it convenient for operators to repair and replace, reducing the time cost of the extraction and purification process, increasing practicality and expanding the scope of application. Attached Figure Description

[0023] Figure 1 This is a front perspective view of a plant exosome extraction and purification machine proposed in this utility model;

[0024] Figure 2 This is a side view of a plant exosome extraction and purification machine proposed in this utility model;

[0025] Figure 3 This is a structural breakdown diagram of the cell wall breaking chamber of a plant exosome extraction and purification machine proposed in this utility model;

[0026] Figure 4 This is a cross-sectional view of the filter box of a plant exosome extraction and purification machine proposed in this utility model;

[0027] Figure 5 This is a schematic diagram illustrating the fixed mechanism structure of a plant exosome extraction and purification machine proposed in this utility model;

[0028] Figure 6 This is a disassembled view of the rotor structure of a plant exosome extraction and purification machine proposed in this utility model.

[0029] Legend:

[0030] 1. Base plate; 2. Fixing mechanism; 201. Support frame; 202. Motor II; 203. Outer shell; 204. Base; 205. Slide groove; 206. Pressing block; 207. Turntable; 208. Column; 209. Displacement groove; 210. Rotor; 211. Test tube trough; 3. Support; 4. Motor I; 5. Blending chamber; 6. Blade; 7. Pectinase solution pool; 8. Filter box; 9. Burette; 10. PBS solution pool; 11. Guide rail; 12. Piston; 13. Slider; 14. Nameplate; 15. Hinge; 16. End cap; 17. Protective cover; 18. Ventilation hole; 19. Control box; 20. Support column; 21. Chassis; 22. Anti-slip pad; 23. Activated carbon plate; 24. Mixing test tube. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] Please see the appendix Figure 1 Appendix Figure 3 and attached Figure 4 An embodiment of this utility model provides a plant exosome extraction and purification machine, comprising a base plate 1, a support 3 fixedly connected to the left side of the base plate 1, a motor 4 fixedly connected to the inner wall of the support 3, a pectinase solution pool 7 fixedly connected to the inner wall of the support 3, a PBS solution pool 10 fixedly connected to the inner wall of the support 3, multiple blades 6 fixedly connected to the output end of the motor 4, a wall-breaking chamber 5 fixedly connected to the top of the support 3, a filter box 8 connected to the outer wall of the wall-breaking chamber 5, multiple activated carbon plates 23 fixedly connected to the inner wall of the filter box 8, a burette 9 connected to the bottom of the filter box 8, a piston 12 fixedly connected to the outer wall of the burette 9, a guide rail 11 fixedly connected to the outer wall of the support 3, a slider 13 slidably connected to the top of the guide rail 11, and a fixing mechanism 2 fixedly connected to the top of the base plate 1 for quick installation.

[0033] Specifically, the support 3 can withstand various forces generated during equipment operation, ensuring long-term stable use of the equipment. The pectinase in the pectinase solution pool 7 can decompose the pectin components in the plant cell wall, destroying the cell wall structure and releasing exosomes from the cell. The phosphate buffer solution in the PBS solution pool 10 provides a suitable physiological environment for the extraction of exosomes. The blade 6 can efficiently break and shear the plant material during rotation, fully breaking down the plant cells and releasing the exosomes. The piston 12 can precisely control the flow rate of the burette 9, ensuring that the extract can drip out at a stable flow rate, facilitating subsequent collection and purification operations.

[0034] Please see the appendix Figure 1 Appendix Figure 5 and attached Figure 6 The fixing mechanism 2 includes a support frame 201, the top of which is fixedly connected to the bottom of the base plate 1. A motor 202 is fixedly connected to the top of the support frame 201. A turntable 207 is fixedly connected to the output end of the motor 202. Multiple displacement grooves 209 are provided on the inner wall of the turntable 207. Columns 208 are slidably connected to the inner wall of the multiple displacement grooves 209. Pressure blocks 206 are fixedly connected to the bottom of the multiple columns 208. A base 204 is slidably connected to the bottom of the turntable 207. A sliding groove 205 is provided on the top of the base 204. A shell 203 is rotatably connected to the outer wall of the base 204. A rotor 210 is slidably connected to the outer wall of the multiple pressure blocks 206. Multiple test tube grooves 211 are provided on the top of the rotor 210.

[0035] Specifically, the support frame 201 ensures that the motor 202 will not shake or shift during operation, thereby ensuring the accuracy of the extraction and purification process. The outer shell 203 protects the internal components and prevents dust and impurities from entering and affecting the normal operation of the mechanism. The displacement groove 209 and the column 208 cooperate to convert the rotational motion into horizontal motion. The pressure block 206 cooperates with the groove on the rotor 210 to firmly fix the rotor 210 and prevent it from shifting.

[0036] Please see the appendix Figure 1 and attached Figure 2 The outer wall of the blending box 5 is fixedly connected to a hinge 15, and the right side of the hinge 15 is fixedly connected to an end cap 16. The inner wall of the bracket 3 is fixedly connected to a protective cover 17, and the outer wall of the protective cover 17 has multiple ventilation holes 18. The left side of the bracket 3 is fixedly connected to a nameplate 14, and the inner wall of the slider 13 is slidably connected to a mixing test tube 24.

[0037] Specifically, the end cap 16 prevents residue from splashing out during the cell wall breaking process, the hinge 15 facilitates the inspection and maintenance of the mechanism by the staff later, the protective cover 17 prevents other substances from entering and affecting the operation of motor 4, the ventilation hole 18 can dissipate the heat generated by motor 4 in a timely manner, and the nameplate 14 records the functions and performance of the equipment, which is convenient for later operation.

[0038] Please see the appendix Figure 1 and attached Figure 2 The bottom of the base plate 1 is fixedly connected to multiple support columns 20, the top of the base plate 1 is fixedly connected to a control box 19, the bottom of the multiple support columns 20 is fixedly connected to a chassis 21, the bottom of the multiple chassis 21 is fixedly connected to an anti-slip pad 22, the bottom of the guide rail 11 is fixedly connected to the top of the base plate 1, and the outer wall of the filter box 8 is fixedly connected to the inner wall of the bracket 3.

[0039] Specifically, the control box 19 can control the start and stop of the entire mechanism. The chassis 21 and the anti-slip pad 22 work together to disperse the pressure and vibration generated during the operation of the mechanism, protecting the ground environment and equipment. The slider 13 and the guide rail 11 work together to make the movement more stable. The filter box 8 provides a safe environment for plant cell wall breaking.

[0040] Working principle: When motor 4 is turned on, it drives the blade 6 to rotate at high speed, breaking down the plant cells in the cell wall breaking chamber 5. The broken plant cells flow into the filter chamber 8 and pass through the multi-stage filtration of the activated carbon plate 23, filtering out the residue and pigments in the juice and flowing into the burette 9. The flow rate and dosage of the juice are adjusted by controlling the piston 12, allowing the juice to drip into the mixing test tube 24. The slider 13 is slid to move it left and right on the guide rail 11. Then, a certain amount of PBS solution and pectinase solution are added to mix them thoroughly.

[0041] When motor 202 is turned on, the rotation of motor 202 drives turntable 207 to rotate. The displacement groove 209 on turntable 207 cooperates with column 208 to make column 208 move horizontally. The pressure block 206, which is fixedly connected to column 208, also moves horizontally in the sliding groove 205 on base 204, so that the pressure block 206 is stuck in the notch at the bottom of rotor 210, fixing rotor 210. Reverse motor 202, and the pressure block 206 moves to all sides, loosening rotor 210 for easy maintenance and replacement.

[0042] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A plant exosome extraction and purification machine, comprising a base plate (1), characterized in that: A bracket (3) is fixedly connected to the left side of the base plate (1). A motor (4) is fixedly connected to the inner wall of the bracket (3). A pectinase solution pool (7) is fixedly connected to the inner wall of the bracket (3). A PBS solution pool (10) is fixedly connected to the inner wall of the bracket (3). Multiple blades (6) are fixedly connected to the output end of the motor (4). A wall-breaking box (5) is fixedly connected to the top of the bracket (3). A filter box (8) is connected to the outer wall of the wall-breaking box (5). Multiple activated carbon plates (23) are fixedly connected to the inner wall of the filter box (8). A burette (9) is connected to the bottom of the filter box (8). A piston (12) is fixedly connected to the outer wall of the burette (9). A guide rail (11) is fixedly connected to the outer wall of the bracket (3). A slider (13) is slidably connected to the top of the guide rail (11). A fixing mechanism (2) is fixedly connected to the top of the base plate (1). The fixing mechanism (2) is used for quick installation.

2. The plant exosome extraction and purification machine according to claim 1, characterized in that: The fixing mechanism (2) includes a support frame (201), the top of the support frame (201) is fixedly connected to the bottom of the base plate (1), a second motor (202) is fixedly connected to the top of the support frame (201), a turntable (207) is fixedly connected to the output end of the second motor (202), a plurality of displacement grooves (209) are provided on the inner wall of the turntable (207), a column (208) is slidably connected to the inner wall of the plurality of displacement grooves (209), a pressure block (206) is fixedly connected to the bottom of the plurality of columns (208), a base (204) is slidably connected to the bottom of the turntable (207), a sliding groove (205) is provided on the top of the base (204), a shell (203) is rotatably connected to the outer wall of the base (204), a rotor (210) is slidably connected to the outer wall of the plurality of pressure blocks (206), and a plurality of test tube grooves (211) are provided on the top of the rotor (210).

3. The plant exosome extraction and purification machine according to claim 1, characterized in that: The outer wall of the blending box (5) is fixedly connected to a hinge (15), and an end cap (16) is fixedly connected to the right side of the hinge (15).

4. The plant exosome extraction and purification machine according to claim 1, characterized in that: The inner wall of the bracket (3) is fixedly connected to a protective cover (17), and the outer wall of the protective cover (17) is provided with multiple ventilation holes (18).

5. The plant exosome extraction and purification machine according to claim 1, characterized in that: The bottom of the base plate (1) is fixedly connected to a plurality of support columns (20), and the top of the base plate (1) is fixedly connected to a control box (19).

6. The plant exosome extraction and purification machine according to claim 5, characterized in that: The bottom of the plurality of support columns (20) is fixedly connected to a chassis (21), and the bottom of the plurality of chassis (21) is fixedly connected to an anti-slip pad (22).

7. The plant exosome extraction and purification machine according to claim 1, characterized in that: A nameplate (14) is fixedly connected to the left side of the bracket (3), and a mixing tube (24) is slidably connected to the inner wall of the slider (13).

8. The plant exosome extraction and purification machine according to claim 1, characterized in that: The bottom of the guide rail (11) is fixedly connected to the top of the guide rail (11), and the outer wall of the filter box (8) is fixedly connected to the inner wall of the bracket (3).

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