A kit for the detection of plasmodium by isothermal amplification

Abstract

The utility model discloses a constant temperature amplification detection reagent box of malaria parasite, including constant temperature box, the lower surface fixed connection of constant temperature box has the heating case, the inner bottom fixed connection of heating case has the heating pipe, the inner bottom of constant temperature box is equipped with the convection hole, the inside of heating case is provided with the stirring mechanism, the upper surface of constant temperature box is equipped with the jack, the inner wall fixed connection of constant temperature box has a plurality of test tube cover with the one -to -one of jack, the upper end articulates of constant temperature box has the top dust cover, the inner wall fixed mounting of constant temperature box has temperature sensor. Through setting heating case and constant temperature box etc., utilize heating pipe and cooperate stirring mechanism to heat, can make the water temperature in -house heating case even rise, utilize temperature sensor and monitor water temperature, pass through temperature display again and show water temperature, can be convenient for keep water temperature constant, provide the convenience for constant temperature amplification detection work, whole operation process does not need to move test tube frequently, effectively promoted the detection efficiency, also effectively avoided test tube damage.

Description

Technical Field

[0001] This utility model relates to the field of reagent kit technology, specifically to a thermothermal amplification detection kit for Plasmodium. Background Technology

[0002] Isothermal amplification is a novel nucleic acid detection technology used for rapid and sensitive detection of the presence of Plasmodium. The entire amplification process is carried out under isothermal conditions, eliminating the need for complex temperature cycling equipment such as PCR instruments. It can detect extremely low concentrations of Plasmodium DNA, with a sensitivity of up to 1 copy / μL. The entire detection process is usually completed within 1-3 hours, significantly shortening the detection time.

[0003] However, in the actual testing process, a constant temperature device is required. The test tube containing the reagents needs to be removed from the kit and then placed into the constant temperature device for amplification. The entire operation requires frequent movement of the test tubes, which not only affects the testing efficiency, but also easily causes the test tubes to fall and be damaged during the movement, making the entire test impossible.

[0004] Therefore, to solve the above problems, we propose a thermothermal amplification detection kit for Plasmodium. Utility Model Content

[0005] The purpose of this invention is to provide a thermothermal amplification detection kit for Plasmodium to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a thermostatic amplification detection kit for Plasmodium, comprising a thermostatic chamber, a heating chamber fixedly connected to the lower surface of the thermostatic chamber, a heating tube fixedly connected to the bottom of the heating chamber, a convection hole communicating with the heating chamber being opened at the bottom of the thermostatic chamber, a stirring mechanism being provided inside the heating chamber, multiple insertion holes being opened in a rectangular array on the upper surface of the thermostatic chamber, multiple test tube sleeves corresponding one-to-one with the insertion holes being fixedly connected to the inner wall of the thermostatic chamber, a top dust cover being hinged to the upper end of the thermostatic chamber, handles being rotatably connected to both sides of the thermostatic chamber, a temperature sensor being fixedly installed on the inner wall of the thermostatic chamber, and a lithium battery, a temperature display, and a control panel being respectively provided on the outer surface of the thermostatic chamber.

[0007] Preferably, there are multiple convection holes, and the convection holes are distributed in a rectangular array at the bottom of the constant temperature chamber.

[0008] Preferably, the heating tubes are arranged in a serpentine pattern at the bottom of the heating chamber.

[0009] Preferably, the stirring mechanism includes a power motor, a first stirring rod, multiple first stirring blades, a second stirring rod, multiple second stirring blades, and a belt drive assembly. The first stirring rod and the second stirring rod are rotatably connected to both sides of the inner wall of the heating box. The multiple first stirring blades are equidistantly fixed to the outer surface of the first stirring rod, and the multiple second stirring blades are equidistantly fixed to the outer surface of the second stirring rod. The power motor is fixedly connected to the outer surface of the heating box, and the output end of the power motor is drivenly connected to the first stirring rod. The first stirring rod and the second stirring rod are drivenly connected through the belt drive assembly.

[0010] Preferably, the plurality of first stirring blades and the plurality of second stirring blades are staggered.

[0011] Preferably, the surface of the top dust cover and the surface of the constant temperature chamber are both embedded with corresponding magnetic blocks.

[0012] Preferably, the test tube sleeve is made of rubber, and at least one water storage tank is fixedly connected to the side of the heating box. A limiting rib is fixedly connected to the inner wall of the water storage tank, and a baffle plate corresponding to the limiting rib is slidably connected to the inner wall of the water storage tank. A servo motor is fixedly connected to the end of the water storage tank, and a lead screw that is threadedly connected to the baffle plate is fixedly connected to the output end of the servo motor. A water guide pipe is provided at the end of the water storage tank away from the servo motor. One end of the water guide pipe is connected to the water storage tank, and the other end of the water guide pipe is connected to the inside of the heating box.

[0013] Beneficial effects This invention provides a thermo-amplified detection kit for Plasmodium, which has the following beneficial effects: 1. This isothermal amplification detection kit for Plasmodium utilizes a heating chamber and an incubator. Heating with a heating tube and a stirring mechanism ensures a uniform increase in water temperature within the heating chamber. Convection holes facilitate the entry of hot water into the incubator. A temperature sensor monitors the water temperature, which is then displayed on a temperature display, ensuring a constant water temperature. A top dust cover not only prevents dust accumulation but also effectively controls heat loss, further contributing to temperature stability and facilitating isothermal amplification detection. The entire process eliminates the need for frequent tube movement, significantly improving detection efficiency and preventing tube damage.

[0014] 2. This isothermal amplification detection kit for Plasmodium uses a water storage tank, limiting ribs, a water baffle, a servo motor, a lead screw, and a water guide tube. The servo motor drives the lead screw to rotate, and the limiting ribs limit the water baffle. The threaded connection between the lead screw and the water baffle allows the baffle to slide within the water storage tank. This movement of the baffle forces water from the storage tank through the water guide tube into the heating chamber, increasing the water pressure within the chamber. This increased pressure flattens the rubber tube sleeve, applying pressure to the test tubes and securing them, effectively preventing spillage during transport. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the isothermal amplification detection kit for Plasmodium proposed in this utility model; Figure 2 This is a cross-sectional view of the isothermal amplification detection kit for Plasmodium proposed in this utility model. Figure 3 This is a cross-sectional view of the incubator structure of the isothermal amplification detection kit for malaria parasites proposed in this utility model. Figure 4 This is a top-down sectional view of the heating chamber structure of the isothermal amplification detection kit for malaria parasites proposed in this utility model.

[0016] In the diagram: 1. Constant temperature chamber; 2. Heating chamber; 3. Heating tube; 4. Convection hole; 5. Stirring mechanism; 6. Insertion hole; 7. Test tube sleeve; 8. Top dust cover; 9. Handle; 10. Temperature sensor; 11. Lithium battery; 12. Temperature display; 13. Control panel; 14. Power motor; 15. First stirring rod; 16. First stirring blade; 17. Second stirring rod; 18. Second stirring blade; 19. Belt drive assembly; 20. Magnetic block; 21. Water storage tank; 22. Limiting rib; 23. Water baffle; 24. Servo motor; 25. Water guide pipe; 26. Lead screw. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0019] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0020] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0021] Example 1, please refer to Figure 1-4 This utility model provides a technical solution: a thermostatic amplification detection kit for Plasmodium, including a thermostatic chamber 1, a heating chamber 2 fixedly connected to the lower surface of the thermostatic chamber 1, a heating tube 3 fixedly connected to the inner bottom of the heating chamber 2, a convection hole 4 communicating with the heating chamber 2 opened in the inner bottom of the thermostatic chamber 1, a stirring mechanism 5 arranged inside the heating chamber 2, multiple insertion holes 6 arranged in a rectangular array on the upper surface of the thermostatic chamber 1, multiple test tube sleeves 7 corresponding one-to-one with the insertion holes 6 fixedly connected to the inner wall of the thermostatic chamber 1, a top dust cover 8 hinged to the upper end of the thermostatic chamber 1, handles 9 rotatably connected to both sides of the thermostatic chamber 1, a temperature sensor 10 fixedly installed on the inner wall of the thermostatic chamber 1, and a lithium battery 11, a temperature display 12, and a control panel 13 respectively arranged on the outer surface of the thermostatic chamber 1.

[0022] By setting up a heating chamber 2 and a constant temperature chamber 1, and using heating tubes 3 for heating in conjunction with a stirring mechanism 5, the water temperature in the heating chamber 2 can be raised evenly. The convection holes 4 facilitate the entry of hot water into the constant temperature chamber 1. The water temperature is monitored by a temperature sensor 10 and displayed on a temperature display 12, which helps to maintain a constant water temperature. The top dust cover 8 not only prevents dust but also effectively prevents heat loss, thus contributing to a constant temperature and facilitating isothermal amplification detection. The entire operation does not require frequent movement of test tubes, effectively improving detection efficiency and preventing test tube damage.

[0023] There are multiple convection holes 4, and the convection holes 4 are distributed in a rectangular array at the bottom of the constant temperature chamber 1. By setting multiple convection holes 4, the water in the heating chamber 2 and the constant temperature chamber 1 can be easily convectioned, which is conducive to water flow and effectively avoids local water temperature from being too high.

[0024] The heating tubes 3 are arranged in a serpentine pattern at the bottom of the heating chamber 2. This arrangement helps to heat the water in the heating chamber 2 evenly.

[0025] The stirring mechanism 5 includes a power motor 14, a first stirring rod 15, multiple first stirring blades 16, a second stirring rod 17, multiple second stirring blades 18, and a belt drive assembly 19. The first stirring rod 15 and the second stirring rod 17 are rotatably connected to both sides of the inner wall of the heating box 2. The multiple first stirring blades 16 are equidistantly fixed to the outer surface of the first stirring rod 15, and the multiple second stirring blades 18 are equidistantly fixed to the outer surface of the second stirring rod 17. The power motor 14 is fixedly connected to the outer surface of the heating box 2, and the output end of the power motor 14 is drively connected to the first stirring rod 15. The first stirring rod 15 and the second stirring rod 17 are connected by a belt drive assembly 19. The first stirring blade 16 and multiple second stirring blades 18 are staggered. By setting the stirring mechanism 5 and using the power motor 14, the first stirring rod 15 and multiple first stirring blades 16 can be rotated. Through the belt drive assembly 19, the second stirring rod 17 and the second stirring blades 18 can be rotated. The rotation of multiple first stirring blades 16 and multiple second stirring blades 18 can disturb the water in the heating tank 2, thereby effectively avoiding excessively high or low local water temperature and facilitating the maintenance of stable water temperature.

[0026] Both the surface of the top dust cover 8 and the surface of the constant temperature chamber 1 are embedded with corresponding magnetic blocks 20. By setting the magnetic blocks 20, the top dust cover 8 can be easily closed.

[0027] Example 2, please refer to Figure 1-4Including Embodiment 1, and based on Embodiment 1, this utility model provides a technical solution: the test tube sleeve 7 is made of rubber, at least one water storage tank 21 is fixedly connected to the side of the heating box 2, a limiting rib 22 is fixedly connected to the inner wall of the water storage tank 21, a baffle plate 23 corresponding to the limiting rib 22 is slidably connected to the inner wall of the water storage tank 21, a servo motor 24 is fixedly connected to the end of the water storage tank 21, a screw 26 threadedly connected to the baffle plate 23 is fixedly connected to the output end of the servo motor 24, and a water guide pipe 25 is provided at the end of the water storage tank 21 away from the servo motor 24, one end of the water guide pipe 25 is connected to the water storage tank 21, and the other end of the water guide pipe 25 is connected to the inside of the heating box 2.

[0028] By setting up a water storage tank 21, a limiting shank 22, a water baffle 23, a servo motor 24, a lead screw 26, and a water guide pipe 25, the servo motor 24 drives the lead screw 26 to rotate. The limiting shank 22 limits the water baffle. The lead screw 26 and the water baffle 23 are threaded together, allowing the water baffle 23 to slide inside the water storage tank 21. As the water baffle 23 moves, water in the water storage tank 21 is squeezed from the water guide pipe 25 into the heating chamber 2, thereby increasing the water pressure inside the constant temperature chamber 1. By increasing the water pressure inside the constant temperature chamber 1, the rubber test tube sleeve 7 is flattened, thus applying pressure to the test tubes inside the sleeve 7 and fixing them, effectively preventing the test tubes from shaking during transportation and causing reagents to spill.

[0029] The handle 9 allows for easy carrying of the entire reagent kit. The entire reagent kit can be powered by the lithium battery 11 to the heating tube 3, the power motor 14, and the servo motor 24. The heating tube 3, the power motor 14, and the servo motor 24 can be controlled via the control panel 13. The entire reagent kit is compact and easy to carry.

[0030] Working principle: When using the isothermal amplification detection kit for Plasmodium, insert the test tube containing the reagent into the test tube sleeve 7 through the insertion hole 6, then close the top dust cover 8, and turn on the heating tube 3, the power motor 14, and the servo motor 24 through the control panel 13. The servo motor 24 works to squeeze the water in the water storage tank 21 into the isothermal chamber 1, thereby pressing the test tube sleeve 7 tightly and fixing the test tube. The power motor 14 drives the first stirring blade 16 and the second stirring blade 18 to rotate, which, together with the heating tube 3, heats the water in the heating chamber 2, keeping the water in the isothermal chamber 1 at a constant temperature. The water temperature is monitored by the temperature sensor 10 and displayed on the temperature display 12, which helps to keep the water temperature constant, thus facilitating isothermal amplification detection. The entire operation does not require frequent movement of the test tube, effectively improving detection efficiency and also effectively avoiding test tube damage.

[0031] The accompanying drawings of the embodiments disclosed in this utility model only involve the structures involved in the embodiments disclosed in this utility model. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of this utility model can be combined with each other. Finally: The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A thermostatic amplification detection kit for Plasmodium, comprising a thermostatic incubator (1), characterized in that: The lower surface of the constant temperature box (1) is fixedly connected to a heating box (2), the bottom of the heating box (2) is fixedly connected to a heating tube (3), the bottom of the constant temperature box (1) is provided with a convection hole (4) that connects to the heating box (2), the heating box (2) is provided with a stirring mechanism (5), the upper surface of the constant temperature box (1) is provided with multiple insertion holes (6) in a rectangular array, the inner wall of the constant temperature box (1) is fixedly connected with multiple test tube sleeves (7) that correspond one-to-one with the insertion holes (6), the upper end of the constant temperature box (1) is hinged with a top dust cover (8), the two sides of the constant temperature box (1) are rotatably connected with handles (9), the inner wall of the constant temperature box (1) is fixedly installed with a temperature sensor (10), and the outer surface of the constant temperature box (1) is provided with a lithium battery (11), a temperature display (12) and a control panel (13).

2. The isothermal amplification detection kit for Plasmodium according to claim 1, characterized in that: The number of convection holes (4) is multiple, and the convection holes (4) are distributed in a rectangular array at the bottom of the constant temperature chamber (1).

3. The isothermal amplification detection kit for Plasmodium according to claim 1, characterized in that: The heating tubes (3) are distributed in a serpentine pattern at the bottom of the heating box (2).

4. The isothermal amplification detection kit for Plasmodium according to claim 1, characterized in that: The stirring mechanism (5) includes a power motor (14), a first stirring rod (15), multiple first stirring blades (16), a second stirring rod (17), multiple second stirring blades (18), and a belt drive assembly (19). The first stirring rod (15) and the second stirring rod (17) are rotatably connected to the inner wall of the heating box (2) respectively. Multiple first stirring blades (16) are equidistantly fixed to the outer surface of the first stirring rod (15), and multiple second stirring blades (18) are equidistantly fixed to the outer surface of the second stirring rod (17). The power motor (14) is fixedly connected to the outer surface of the heating box (2), and the output end of the power motor (14) is drivenly connected to the first stirring rod (15). The first stirring rod (15) and the second stirring rod (17) are drivenly connected through the belt drive assembly (19).

5. The isothermal amplification detection kit for Plasmodium according to claim 4, characterized in that: Multiple first stirring blades (16) and multiple second stirring blades (18) are staggered.

6. The isothermal amplification detection kit for Plasmodium according to claim 1, characterized in that: Both the surface of the top dust cover (8) and the surface of the constant temperature chamber (1) are embedded with corresponding magnetic blocks (20).

7. The isothermal amplification detection kit for Plasmodium according to claim 1, characterized in that: The test tube sleeve (7) is made of rubber. At least one water storage tank (21) is fixedly connected to the side of the heating box (2). A limiting rib (22) is fixedly connected to the inner wall of the water storage tank (21). A baffle plate (23) corresponding to the limiting rib (22) is slidably connected to the inner wall of the water storage tank (21). A servo motor (24) is fixedly connected to the end of the water storage tank (21). A screw (26) threadedly connected to the baffle plate (23) is fixedly connected to the output end of the servo motor (24). A water guide pipe (25) is provided at the end of the water storage tank (21) away from the servo motor (24). One end of the water guide pipe (25) is connected to the water storage tank (21), and the other end of the water guide pipe (25) is connected to the inside of the heating box (2).

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