A living cell culture pod
By designing a live cell culture chamber and utilizing temperature, humidity, gas, and reagent control modules, a suitable living environment for cells is provided, solving the problem of cells being separated from their growth environment during observation and improving the accuracy of observation results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- NORTHWEST INST OF PLATEAU BIOLOGY CHINESE ACAD OF SCI
- Filing Date
- 2025-08-25
- Publication Date
- 2026-07-24
AI Technical Summary
In existing technologies, cells are separated from their original growth environment when observed between a glass slide and a coverslip, leading to inaccurate observation results.
Design a live cell culture chamber comprising a sealed chamber, a temperature control module, a humidity control module, a gas control module, and a reagent perfusion module to provide a suitable living environment for cells and ensure that cells remain in a normal state during observation.
Modular control ensures that cells remain in a normal state during observation, improving the accuracy of observation results.
Smart Images
Figure CN224548436U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell culture technology, and in particular to a live cell culture chamber. Background Technology
[0002] In existing technologies, when observing cells using a microscope, cells are typically placed on a glass slide, and then a coverslip is placed on top of the cells to form a cell specimen. The resulting cell specimen is then placed on a stage for observation. However, placing the cells between the glass slide and the coverslip removes them from their original growth environment, preventing them from living normally. Therefore, cells observed in this manner are not in a normal state, affecting the accuracy of the observation results. Consequently, there is an urgent need for a device that can improve the accuracy of cell observation results and solve the problems existing in the prior art. Utility Model Content
[0003] The purpose of this invention is to provide a live cell culture chamber to improve the accuracy of cell observation results and ensure that the cells are in a normal state when being observed.
[0004] To achieve the above objectives, this utility model provides the following solution:
[0005] A live cell culture chamber, comprising:
[0006] A sealed chamber used to hold cell culture dishes;
[0007] A temperature control module, connected to the sealed chamber, is used to control the internal temperature of the sealed chamber;
[0008] A humidity control module, connected to the sealed chamber, is used to control the humidity inside the sealed chamber;
[0009] A gas control module, connected to the sealed chamber, is used to control the gas concentration and gas type inside the sealed chamber;
[0010] A reagent perfusion module, connected to the cell culture dish, is used to control the type and flow rate of reagents delivered into the cell culture dish.
[0011] Furthermore, the temperature control module includes: a temperature controller, a temperature sensor, a power supply, and an inner wall heating tube. The inner wall heating tube is disposed inside the wall of the sealed chamber and is used to heat the sealed chamber. The power supply is electrically connected to the inner wall heating tube. The temperature sensor is disposed on the sealed chamber, and the temperature sensor and the inner wall heating tube are electrically connected to the temperature controller.
[0012] Furthermore, the temperature control module also includes a gas heating tube, which is disposed inside the sealed chamber and used to heat the gas inside the sealed chamber. The gas heating tube is electrically connected to the power supply and the temperature controller.
[0013] Furthermore, the humidity control module includes: a humidity sensor, a humidity controller, and a humidifier. The humidifier is connected to the sealed chamber, the humidity sensor is disposed inside the sealed chamber, and the humidity sensor and the humidifier are electrically connected to the humidity controller.
[0014] Furthermore, the gas control module includes: multiple gas cylinders, multiple gas pumps, multiple gas concentration sensors, and a gas concentration controller. The multiple gas concentration sensors are disposed inside the sealed chamber. Each gas cylinder is connected to one of the gas pumps, and the multiple gas pumps and the multiple gas concentration sensors are electrically connected to the gas concentration controller.
[0015] Furthermore, the reagent perfusion module includes: a pressure-driven pump, a reagent storage chamber, and a multi-way switching valve. The pressure-driven pump is connected to the reagent storage chamber, and the reagent storage chamber is connected to the cell culture dish through the multi-way switching valve.
[0016] Furthermore, the device also includes an observation window, which is disposed on the sealed chamber.
[0017] Furthermore, the sealed chamber is made of aluminum alloy.
[0018] Furthermore, an installation groove is provided on the inner wall of the bottom of the sealed chamber, a first magnetic connection layer is provided at the bottom of the installation groove, and a second magnetic connection layer is provided on the bottom surface of the cell culture dish, the second connection layer being magnetically connected to the first connection layer.
[0019] Furthermore, the mounting groove includes culture plate grooves and culture dish grooves of different sizes for placing cell culture dishes of different sizes.
[0020] According to the above description, this utility model has the following technical effects:
[0021] This invention provides a live cell culture chamber, comprising: a sealed chamber for holding cell culture dishes; a temperature control module connected to the sealed chamber for controlling the internal temperature of the sealed chamber; a humidity control module connected to the sealed chamber for controlling the internal humidity of the sealed chamber; a gas control module connected to the sealed chamber for controlling the gas concentration and type inside the sealed chamber; and a reagent perfusion module connected to the cell culture dishes for controlling the type and flow rate of reagents delivered into the cell culture dishes. These four modules provide a suitable living environment for live cells, enabling them to maintain a normal cellular state during observation, thus improving the accuracy of cell observation results. Attached Figure Description
[0022] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0023] Figure 1 This is a schematic diagram illustrating the usage scenario of the live cell culture chamber provided in the embodiments of this specification;
[0024] Figure 2 This is a schematic diagram of the principle structure of the live cell culture chamber provided in the embodiments of this specification;
[0025] Among them, 1-microscope observation opening; 2-culture dish trough; 3-culture plate trough; 4-sealed chamber; 5-glass slide trough. Detailed Implementation
[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0027] like Figure 2 As shown in the embodiments of this specification, a live cell culture chamber is provided, comprising:
[0028] Sealed chamber 4, used to hold cell culture dishes;
[0029] A temperature control module, connected to the sealed chamber 4, is used to control the internal temperature of the sealed chamber 4.
[0030] A humidity control module, connected to the sealed chamber 4, is used to control the humidity inside the sealed chamber 4;
[0031] The gas control module is connected to the sealed chamber 4 and is used to control the gas concentration and gas type inside the sealed chamber 4.
[0032] The reagent perfusion module, connected to the cell culture dish, controls the type and flow rate of reagents delivered into the cell culture dish. A schematic diagram illustrating the use case of the live cell culture chamber is shown below. Figure 1 As shown, a live cell culture chamber is used instead of a stage for observing live cells. These four modules provide a suitable environment for the cells, allowing them to maintain a normal cellular state during observation, thus improving the accuracy of cell observation results.
[0033] The temperature control module includes a temperature controller, a temperature sensor, a power supply, and an inner wall heating element. The inner wall heating element is located inside the walls of the sealed chamber 4 and is used to heat the chamber. The power supply is electrically connected to the inner wall heating element. The temperature sensor is located on the sealed chamber 4 and is electrically connected to the temperature controller along with the inner wall heating element to control the temperature of the inner wall. The temperature control module also includes a gas heating element, located inside the sealed chamber 4, used to heat the gas inside the chamber. The gas heating element is electrically connected to the power supply and the temperature controller to control the temperature of the gas environment inside the chamber. Alternatively, other widely used temperature control modules in existing technologies can be selected. The temperature control module can achieve temperature control between room temperature and 50 degrees Celsius with a control accuracy of 0.1 degrees Celsius. The inner wall heating element is installed by embedding transparent metal oxide heating glass within the chamber to heat the inner wall, ensuring uniform temperature.
[0034] The humidity control module includes a humidity sensor, a humidity controller, and a humidifier. The humidifier is connected to the sealed chamber 4, and the humidity sensor is located inside the sealed chamber 4. The humidity sensor and the humidifier are electrically connected to the humidity controller to control the humidity inside the chamber and ensure that the cells are in a suitable humidity environment. Alternatively, other humidity control modules widely used in existing technologies can be selected. The humidifier heats water to produce water vapor, which is then introduced into the chamber to increase the humidity inside. The humidity controller can control the humidity inside the chamber by controlling the rate at which pure water in the humidifier vaporizes into water vapor.
[0035] The gas control module includes multiple gas cylinders, multiple gas pumps, multiple gas concentration sensors, and a gas concentration controller. The gas concentration sensors are located inside the sealed chamber 4. Each gas cylinder is connected to one gas pump. The multiple gas pumps and gas concentration sensors are electrically connected to the gas concentration controller to control the gas concentration within the chamber. Alternatively, a widely used gas control module for controlling gas concentration can be selected. This module can achieve gas concentration control from 0% to 100%, with a control accuracy of 1%.
[0036] The reagent perfusion module includes a pressure-driven pump, a reagent storage chamber, and a multi-way switching valve. The pressure-driven pump is connected to the reagent storage chamber, which is connected to a small hole in the cell culture dish via the multi-way switching valve. This allows for the supply of reagents to the cell culture dish, enabling unidirectional flow of different types of reagents within the cell culture dish for easy cell observation. Alternatively, a widely used liquid perfusion module can be selected based on specific needs.
[0037] The device also includes an observation window, which is located on the sealed chamber 4, for observing the cells inside the chamber 4. The observation window is made of high-transmittance quartz.
[0038] The sealed compartment 4 is preferably made of aluminum alloy, but other materials widely used in compartment construction can also be used.
[0039] An installation groove is provided on the inner wall of the bottom of the sealed chamber 4. A first magnetic connection layer is provided at the bottom of the installation groove, and a second magnetic connection layer is provided on the bottom surface of the cell culture dish. The second connection layer is magnetically connected to the first connection layer. This arrangement makes it easy to fix the position of the cell culture dish so that it will not move easily and facilitates subsequent observation of live cells.
[0040] The mounting compartment includes culture plate compartments 3 and culture dish compartments 2 of different sizes for holding cell culture dishes of different sizes, thereby improving the adaptability of the live cell culture chamber. The mounting compartment also includes a slide compartment 5 for holding slides. A microscope observation opening 1 is provided on the sealed chamber 4 at a position corresponding to the center of the mounting compartment, for observing live cells through a microscope. The size of the culture dish compartment 2 can be set according to actual needs, preferably circular.
[0041] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the systems disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the descriptions are relatively simple; relevant parts can be referred to the method section.
[0042] This document uses specific examples to illustrate the principles and implementation methods of this utility model. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this utility model. Furthermore, those skilled in the art will recognize that, based on the ideas of this utility model, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this utility model.
Claims
1. A live cell culture chamber, characterized in that, include: A sealed chamber used to hold cell culture dishes; A temperature control module, connected to the sealed chamber, is used to control the internal temperature of the sealed chamber; A humidity control module, connected to the sealed chamber, is used to control the humidity inside the sealed chamber; A gas control module, connected to the sealed chamber, is used to control the gas concentration and gas type inside the sealed chamber; A reagent perfusion module, connected to the cell culture dish, is used to control the type and flow rate of reagents delivered into the cell culture dish.
2. The live cell culture chamber according to claim 1, characterized in that, The temperature control module includes: a temperature controller, a temperature sensor, a power supply, and an inner wall heating tube. The inner wall heating tube is disposed inside the wall of the sealed chamber and is used to heat the sealed chamber. The power supply is electrically connected to the inner wall heating tube. The temperature sensor is disposed on the sealed chamber. The temperature sensor and the inner wall heating tube are electrically connected to the temperature controller.
3. The live cell culture chamber according to claim 2, characterized in that, The temperature control module also includes a gas heating tube, which is disposed inside the sealed chamber and is used to heat the gas inside the sealed chamber. The gas heating tube is electrically connected to the power supply and the temperature controller.
4. The live cell culture chamber according to claim 1, characterized in that, The humidity control module includes a humidity sensor, a humidity controller, and a humidifier. The humidifier is connected to the sealed chamber, the humidity sensor is located inside the sealed chamber, and the humidity sensor and the humidifier are electrically connected to the humidity controller.
5. The live cell culture chamber according to claim 1, characterized in that, The gas control module includes: multiple gas cylinders, multiple gas pumps, multiple gas concentration sensors, and a gas concentration controller. The multiple gas concentration sensors are disposed inside the sealed chamber. Each gas cylinder is connected to one of the gas pumps, and the multiple gas pumps and the multiple gas concentration sensors are electrically connected to the gas concentration controller.
6. The live cell culture chamber according to claim 1, characterized in that, The reagent perfusion module includes a pressure-driven pump, a reagent storage chamber, and a multi-way switching valve. The pressure-driven pump is connected to the reagent storage chamber, and the reagent storage chamber is connected to the cell culture dish through the multi-way switching valve.
7. The live cell culture chamber according to any one of claims 1 to 6, characterized in that, It also includes an observation window, which is located on the sealed cabin.
8. The live cell culture chamber according to claim 1, characterized in that, The sealed chamber is made of aluminum alloy.
9. The live cell culture chamber according to claim 8, characterized in that, An installation groove is provided on the inner wall of the bottom of the sealed chamber. A first magnetic connection layer is provided at the bottom of the installation groove. A second magnetic connection layer is provided on the bottom surface of the cell culture dish. The second magnetic connection layer is magnetically connected to the first magnetic connection layer.
10. The live cell culture chamber according to claim 9, characterized in that, The mounting slots include culture plate slots of different sizes and culture dish slots of different sizes, for placing cell culture dishes of different sizes.