Air jacket incubator

Abstract

The application provides a gas-jacket type incubator, a mounting cavity is arranged in the shell, a sealing door is hinged to the shell, an inner container is arranged on the front side wall, the inner container is fixed in the mounting cavity, a silica gel heating layer is wrapped on the outer wall of the inner container, a first heat preservation layer is wrapped on the outer side of the silica gel heating layer, a support frame is arranged in the inner container, a plurality of culture cavities are arranged in the support frame, the support frame and the corresponding inner wall of the inner container are arranged in a spaced mode to form a gas-jacket cavity, the first air holes are arranged on the support frame, and a fan is arranged at the position close to the top of the inner container. The silica gel heating layer is wrapped on the outer wall of the inner container, the good flexibility of the silica gel heating layer can tightly adhere to the inner container, the uniform heating of the inner container is realized, the local overheating is avoided, the air in the culture cavity is drawn out from the first air holes on the top of the support frame by the fan, and then the air is returned to the culture cavity through the gas-jacket cavity and the side wall of the support frame, so that the hot air in the inner container can be fully circulated, and the problem of uneven heat distribution of the traditional gas-jacket type incubator is solved.

Description

Technical Field

[0001] This application belongs to the field of incubator technology, and more specifically, relates to an air-jacketed incubator. Background Technology

[0002] A carbon dioxide incubator is a precision instrument that simulates the in vivo environment of organisms and is used for the in vitro culture of cells, tissues, microorganisms, etc. It is widely used in life sciences, medicine, pharmacy and other fields.

[0003] Existing carbon dioxide incubators are divided into water-jacketed and air-jacketed types. Among them, the water jacket of the water-jacketed incubator is a closed or semi-closed structure. After long-term water storage, scale and microbial communities may adhere to the inner wall, making it difficult to clean thoroughly. If the water is not changed in time, pollutants may affect the cleanliness of the chamber through heat exchange, posing a potential threat to the culture of sensitive cells such as stem cells and primary cells.

[0004] Therefore, air-jacketed incubators are becoming increasingly popular. However, in existing air-jacketed incubators, the temperature in areas near the heater, such as the back or bottom, is slightly higher than in areas far from the heating source. Furthermore, there are dead zones in air circulation, resulting in uneven heat distribution within the incubator. Utility Model Content

[0005] The purpose of this application is to provide an air-jacketed incubator to improve the heating uniformity of the incubator.

[0006] To achieve the above objectives, the technical solution adopted in this application is as follows: An air-jacketed incubator is provided, comprising a shell, an inner liner, a silicone heating layer, a first insulation layer, a support frame, and a fan. The shell has an internal mounting cavity with an opening at its front end. A sealing door is hinged to the shell, allowing the mounting cavity to be opened or closed by flipping. The inner liner has an opening on its front side wall and is fixed within the mounting cavity. At least the left side wall, right side wall, and top wall of the inner liner are spaced apart from the inner wall corresponding to the mounting cavity to form an air-jacketed cavity. The silicone heating layer… The inner liner is covered with an outer wall; a first insulation layer covers the outside of the silicone heating layer; a support frame is installed inside the inner liner, and the support frame has multiple culture chambers. The left side wall, right side wall, and top wall of the support frame are arranged at intervals with the inner wall of the inner liner to form an air jacket cavity. The top wall, left side wall, and right side wall of the support frame are all provided with first air holes; a fan is located near the top of the inner liner. The fan is used to draw air out of the culture chamber from the top, circulate it through the air jacket cavity, and then return it to the culture chamber through the side wall of the support frame.

[0007] In one possible implementation, the inner liner extends outward and is provided with a fixing plate, and a connecting lug is connected to the fixing plate. Multiple fasteners are provided on the inner wall of the shell, and the fasteners are located near the four corners of the shell. The connecting lugs are connected to the fasteners by bolts.

[0008] In one possible implementation, the support frame includes two vertically arranged air guide plates, a plurality of partitions disposed between the air guide plates, and a top plate disposed between the two air guide plates. A cavity-forming ring is provided on the outer side of the air guide plate, and the cavity-forming ring abuts against the corresponding side wall of the inner liner. A sealing plate is provided between the top plate and the top wall of the inner liner. The inner wall of the inner liner, the air guide plates, the cavity-forming ring, and the sealing plate form a sealed air jacket cavity. A plurality of second air holes are uniformly provided on the partitions.

[0009] In one possible implementation, the density of the first pores gradually decreases from bottom to top.

[0010] In one possible implementation, the air guide plate has mounting holes, a sheet metal part is fitted into the mounting holes, and the portion of the sheet metal part located inside the air guide plate forms an insertion groove, into which the partition plate is inserted.

[0011] In one possible implementation, the bottom of the housing is provided with a plurality of casters, which are located near the four corners of the housing. The mounting base of the casters is provided with support feet that can be raised and lowered, and the support feet can be released from contact with the ground by extending outward.

[0012] In one possible implementation, an installation tube is provided through the inner liner, the shell, the first insulation layer and the silicone heating layer, the installation tube is sealed to the shell, and the outer end of the installation tube is provided with a sealing plug.

[0013] In one possible implementation, the top of the housing is provided with an operating box, the operating box contains a motor, and the power output end of the motor is connected to the fan; the operating box contains a PLC controller, the PLC controller is electrically connected to the motor, and the PLC controller is used to control the starting and stopping of the motor.

[0014] In one possible implementation, a carbon dioxide sensor is installed inside the inner liner and connected to the PLC controller. A gas supply nozzle is installed on the side wall of the control box. The outer end of the gas supply nozzle is connected to an external carbon dioxide supply device, and the inner end of the gas supply nozzle is connected to a filter. A solenoid valve is connected to the filter. A carbon dioxide supply nozzle passes through the inner liner, the silicone heating layer, and the first insulation layer. The outlet end of the solenoid valve is connected to the inlet end of the carbon dioxide supply nozzle. The solenoid valve is electrically connected to the PLC controller. The PLC controller has a preset carbon dioxide concentration. The PLC controller can open or close the solenoid valve according to the real-time carbon dioxide concentration monitored by the carbon dioxide sensor, so that the carbon dioxide concentration inside the housing is not lower than the preset carbon dioxide concentration in the PLC controller.

[0015] In one possible implementation, the side wall of the control box is provided with a plurality of heat dissipation holes, and the front side wall of the control box is provided with an operation panel.

[0016] The beneficial effects of the air-jacketed incubator provided in this application are as follows: Compared with the prior art, this application, through the cooperation of the shell and the sealing door, not only provides a solid support frame for the entire incubator, but also allows for the effective opening and closing of the installation cavity through the flipping of the sealing door, facilitating the operation of operators to put in and take out culture samples. More importantly, in the closed state, it can minimize the exchange between the internal environment and the external environment, ensuring the stability of key culture parameters such as temperature, humidity, and carbon dioxide concentration, providing a growth environment for cells and other biological samples free from external interference. The air-jacketed cavity formed between the inner liner and the support frame, combined with the design of the silicone heating layer, overturns the limitations of traditional heating methods. The silicone heating layer covers the outer wall of the inner liner, and its good flexibility allows it to fit tightly against the inner liner, achieving uniform heating of the inner liner and avoiding local overheating. At the same time, the first insulation layer covers the outside of the silicone heating layer, greatly reducing heat loss, improving energy utilization efficiency, reducing the operating energy consumption of the equipment, and maintaining a stable internal temperature for a long time, creating favorable conditions for the culture of sensitive cells. The setting of the first air vent, in conjunction with the action of the fan, constructs a highly efficient air circulation system. The fan draws air from the top of the culture chamber through the first vent, circulates it through the air jacket, and then returns it to the culture chamber through the first vents on the left and right sides of the support frame. Since hot air accumulates at the top of the inner chamber, the circulation process of this application allows the hot air in the inner chamber to circulate fully, ensuring that the heat is evenly distributed in the culture chamber. This solves the problem of uneven heat distribution in traditional air-jacketed incubators and also allows gases such as carbon dioxide to mix fully in the inner chamber, ensuring a consistent gas environment in each culture area and greatly improving the success rate and stability of the culture. Attached Figure Description To more clearly illustrate the technical solutions in the embodiments of this application, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 A schematic diagram of the working structure of the air-jacketed incubator provided in the embodiment of this application; Figure 2 This is a schematic diagram of the internal structure of the air-jacketed incubator provided in an embodiment of this application; Figure 3 This is a schematic diagram of the internal structure of the inner liner provided in an embodiment of this application; Figure 4 This is a schematic diagram of the structure of the caster wheel provided in an embodiment of this application; Figure 5 A cross-sectional view of the air-jacketed incubator provided in an embodiment of this application is a structural schematic diagram. Figure 6 for Figure 5 Enlarged view of part A.

[0018] The labels for the attached figures are as follows: 1. Shell; 2. Inner liner; 3. Silicone heating layer; 4. First insulation layer; 5. Support frame; 6. Fan; 7. Control box; 101. Sealing door; 102. Air jacket cavity; 103. Fastener; 104. Casters; 105. Mounting base; 106. Support leg; 107. Mounting tube; 201. Connecting ear; 202. Carbon dioxide sensor; 501. Air guide plate; 502. Partition plate; 503. Cavity ring; 504. Top plate; 505. First air hole; 506. Second air hole; 507. Sheet metal part; 508. Sealing plate; 701. Motor; 702. Air supply nozzle; 703. Filter; 704. Solenoid valve; 705. Heat dissipation hole; 706. Control panel; 707. First sampling nozzle. Detailed Implementation

[0019] To make the technical problems, technical solutions, and beneficial effects to be solved by this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and are not intended to limit the scope of this application.

[0020] It should be further noted that the accompanying drawings and embodiments of this application mainly describe the concept of this application. Based on this concept, some specific forms and arrangements of connection relationships, positional relationships, power mechanisms, power supply systems, hydraulic systems and control systems may not be fully described. However, under the premise that those skilled in the art understand the concept of this application, they can implement the above-mentioned specific forms and arrangements in a well-known manner.

[0021] When a component is referred to as being "fixed to" or "set on" another component, it can be directly on or indirectly on that other component. When a component is referred to as being "connected to" another component, it can be directly connected to or indirectly connected to that other component.

[0022] The terms “length”, “width”, “up”, “down”, “front”, “back”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inner”, “outer”, etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0023] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means two or more, and "several" means one or more, unless otherwise explicitly specified.

[0024] The air-jacketed incubator provided in this application will now be described.

[0025] Please refer to the following: Figures 1 to 6The air-jacketed incubator includes a shell 1, an inner liner 2, a silicone heating layer 3, a first insulation layer 4, a support frame 5, and a fan 6. The shell 1 has an internal mounting cavity with an opening at its front end. A sealing door 101 is hinged to the shell 1, allowing the mounting cavity to be opened or closed by flipping it. The inner liner 2 has an opening on its front side wall and is fixed within the mounting cavity. At least its left, right, and top walls are spaced apart from the inner walls of the mounting cavity to form an air-jacketed cavity 102. The silicone heating layer 3 covers the inner liner 2. On the outer wall; the first insulation layer 4 covers the outside of the silicone heating layer 3; the support frame 5 is located inside the inner liner 2, and the support frame 5 has multiple culture chambers. The left side wall, right side wall and top wall of the support frame 5 are arranged at intervals with the inner wall of the inner liner 2 to form an air jacket cavity 102. The top wall, left side wall and right side wall of the support frame 5 are provided with first air holes; the fan 6 is located near the top of the inner liner 2. The fan 6 is used to draw the air in the culture chamber from the top, circulate it through the air jacket cavity 102 and then return it to the culture chamber through the side wall of the support frame 5.

[0026] The beneficial effects of the air-jacketed incubator provided in this embodiment are as follows: Compared with the prior art, the air-jacketed incubator provided in this embodiment, through the cooperation of the shell 1 and the sealing door 101, not only provides a solid support frame for the entire incubator, but also enables the effective opening and closing of the installation cavity by flipping the sealing door 101, which facilitates the operation of operators to take out and put in culture samples. More importantly, in the closed state, it can minimize the exchange between the environment inside the chamber and the outside world, ensure the stability of key culture parameters such as temperature, humidity, and carbon dioxide concentration, and provide a growth environment for biological samples such as cells that is not disturbed by the outside world.

[0027] The air jacket cavity 102 formed between the inner liner 2 and the support frame 5, combined with the design of the silicone heating layer 3, overturns the limitations of traditional heating methods. The silicone heating layer 3 covers the outer wall of the inner liner 2, and its excellent flexibility allows it to fit tightly against the inner liner 2, achieving uniform heating and avoiding localized overheating. Simultaneously, the first insulation layer 4 covers the outside of the silicone heating layer 3, greatly reducing heat loss, improving energy efficiency, lowering equipment operating energy consumption, and maintaining stable internal temperature for extended periods, creating favorable conditions for the culture of sensitive cells. The placement of the first air vent, in conjunction with the fan 6, constructs a highly efficient air circulation system.

[0028] The fan 6 draws air from the top of the culture chamber through the first air hole, circulates through the air jacket cavity 102, and then returns to the culture chamber through the first air holes on the left and right sides of the support frame 5. Since hot air accumulates at the top of the inner liner 2, the circulation process of this application allows the hot air in the inner liner 2 to circulate fully, so that the heat is evenly distributed in the culture chamber. This solves the problem of uneven heat distribution in traditional air jacket incubators and also allows gases such as carbon dioxide to mix fully in the inner liner, ensuring a consistent gas environment in each culture area, which greatly improves the success rate and stability of the culture.

[0029] The so-called silicone heating layer 3 comprises a silicone substrate, a heating element, and an insulating layer. The silicone substrate, serving as the supporting structure, is typically made of silicone rubber and possesses excellent flexibility, high-temperature resistance, and chemical corrosion resistance, allowing the heating layer to conform to the surfaces of objects of various shapes. The heating element usually uses a heating wire made of a metal alloy, such as a nickel-chromium alloy heating wire; metal-etched circuitry or carbon fiber heating elements are also used. These heating elements are embedded in the silicone substrate, and when current passes through them, electrical energy is converted into heat energy. The insulating layer tightly covers the heating element and is generally composed of a composite of silicone rubber and fiberglass cloth; polyimide film is also sometimes used. This layer prevents leakage and ensures safe operation.

[0030] When an electric current passes through a heating element such as a heating wire or carbon fiber, electrical energy is converted into heat energy due to the resistance. Then, the silicone material, with its good thermal conductivity, evenly transfers the heat to the surface of the heating layer, thereby heating the object in contact with it.

[0031] like Figure 5 As shown, the inner liner 2 extends outward and is provided with a fixing plate. A connecting ear 201 is connected to the fixing plate. Multiple fasteners 103 are provided on the inner wall of the shell 1. The fasteners 103 are located near the four corners of the shell 1. The connecting ear 201 is connected to the fasteners 103 by bolts.

[0032] The inner liner 2 is bolted to the fixing member 103 on the shell 1 via a fixing plate and connecting lug 201. This connection method has extremely high stability and reliability. The fixing member 103 is located near the four corners of the shell 1, which can evenly distribute the weight of the inner liner 2 onto the shell 1, avoiding structural deformation caused by excessive local stress. During equipment operation, even if subjected to slight vibration or impact, the inner liner 2 can remain stable without displacement or shaking, thus ensuring the structural integrity of the air jacket cavity 102 and ensuring the smooth operation of the air circulation system. Meanwhile, the bolted connection facilitates the installation and removal of the inner liner 2. When the equipment requires maintenance or repair, or when the inner liner 2 malfunctions, operators can easily remove the bolts and take out the inner liner 2, greatly reducing the difficulty and time cost of maintenance. In addition, this connection structure ensures the precise relative position between the inner liner 2 and the shell 1, avoiding installation deviations that could affect the size of the air jacket cavity 102 and the air circulation effect, further ensuring the performance stability of the incubator.

[0033] Combination Figure 2 , Figure 5 and Figure 6 As shown, the support frame 5 includes two vertically arranged air guide plates 501, a plurality of partitions 502 disposed between the air guide plates 501, and a top plate disposed between the two air guide plates 501. A cavity-forming ring 503 is provided on the outer side of the air guide plate 501. The cavity-forming ring 503 abuts against the corresponding side wall of the inner liner 2. A sealing plate 508 is provided between the top plate 504 and the top wall of the inner liner. The inner wall of the inner liner 2, the air guide plate 501, the cavity-forming ring 503 and the sealing plate 508 form a sealed air jacket cavity 102. A plurality of second air holes 506 are evenly provided on the partitions 502.

[0034] The unique design of the support frame 5 provides a strong guarantee for the uniform distribution of hot air in the inner chamber. The air jacket cavity, which is sealed by the inner walls of the two vertically arranged inner chambers 2, the air guide plate 501, the cavity-forming ring 503, and the sealing plate, ensures that the air in the culture chamber can only circulate through the air jacket cavity and then return to the culture chamber. Multiple first air holes 505 on the air guide plate 501 introduce air into each culture area, while the evenly distributed second air holes 506 on the partition plate 502 allow air to flow further between different culture chambers, breaking the problem of obstructed airflow in traditional multi-layer shelf structures. This structural design allows air to flow freely and from multiple angles within the inner chamber, ensuring ample air exchange in both horizontal and vertical directions. This effectively eliminates temperature dead zones and gas concentration differences within the incubator. For multi-layered culture samples, the culture environment at each layer remains highly consistent, significantly improving the accuracy and reproducibility of culture results. This is particularly suitable for culturing environmentally sensitive stem cells and primary cells.

[0035] As a preferred technical solution, the density of the first pore 505 gradually decreases from bottom to top. This detailed design fully considers the laws of airflow and heat distribution within the incubator. In the incubator, due to the rising nature of hot air, the upper area tends to accumulate heat more easily, resulting in a relatively higher temperature. The higher density of the lower first pore 505 allows more air to enter the lower culture area from the distribution chamber. Since the fan 6 draws away the hot air near the top inside the inner liner 2, more hot air enters the lower area, effectively reducing the temperature difference between the lower and upper parts of the inner liner 2. In this embodiment, the air guide plate 501 has an installation hole, and a sheet metal part 507 is installed in the installation hole. The part of the sheet metal part 507 located inside the air guide plate 501 forms an insertion groove, and the partition plate 502 is inserted into the insertion groove.

[0036] The structure on the air guide plate 501, with the sheet metal part 507 forming an insertion slot for mounting the baffle 502, reflects the flexibility and practicality of the equipment design. The sheet metal part 507 is snapped into the mounting hole of the air guide plate 501, and the insertion slot formed inside it provides a stable mounting position for the baffle 502, while also facilitating the quick installation and removal of the baffle 502. In actual culture work, different experimental needs often require culture spaces of varying sizes and quantities. Operators can easily change the layout of the culture chamber by adjusting the number and position of the partitions 502 according to factors such as the quantity and volume of the cultured samples. This design not only improves the adaptability of the equipment to different experimental scenarios but also reduces the difficulty of adjusting the equipment structure when changing culture containers, saving experimental preparation time and improving laboratory efficiency. In addition, the plug-in connection method ensures the stability of the partitions 502 after installation, preventing displacement due to airflow or slight equipment vibration, thus ensuring the structural stability of the culture chamber.

[0037] like Figure 3 and Figure 4 As shown, the bottom of the housing 1 is provided with multiple casters 104, which are located near the four corners of the housing 1. Support feet 106 are raised and lowered on the mounting base 105 of the casters 104, and the support feet 106 can extend outwards to release the casters 104 from contact with the ground. Specifically, in this embodiment, the mounting base 105 is provided with threaded holes, and a threaded rod is provided on the top of the support foot 106. The threaded rod is screwed into the threaded hole, and the height of the support foot 106 is changed by the length of the threaded rod screwed into the threaded hole.

[0038] The casters 104 at the bottom of the housing 1 greatly facilitate the movement of the incubator. During laboratory layout adjustments, equipment maintenance, or space planning, operators can easily push the incubator to the designated location without expending a lot of manpower and resources. This design significantly improves the flexibility of the equipment, especially for larger and heavier incubators. The adjustable support feet 106 solve the problem of securing the equipment after movement. Once the incubator is in the desired position, adjusting the support feet 106 to extend them releases the casters 104 from the ground, firmly supporting the incubator. This design effectively prevents the incubator from moving due to accidental collisions, personnel movement, or other external forces during use, ensuring its stability. It also avoids fluctuations in the internal culture environment caused by equipment movement, such as temperature changes and liquid spillage, providing reliable protection for the cultured samples and extending the equipment's lifespan.

[0039] like Figure 5 As shown, an installation tube 107 is provided through the inner liner 2, the shell 1, the first insulation layer 4 and the silicone heating layer 3. The installation tube 107 is sealed to the shell 1, and a sealing plug is provided at the outer end of the installation tube 107.

[0040] The mounting tube 107 provides convenient conditions for the functional expansion and precise monitoring of the incubator. The mounting tube 107, which runs through the inner liner 2, the shell 1, the first insulation layer 4, and the silicone heating layer 3, can be used to temporarily install various detection elements such as temperature sensors and humidity sensors. These elements can directly contact the environment inside the inner liner 2, monitor various culture parameters in real time and accurately, provide data support for the precise control of the equipment, and facilitate temporary monitoring of the environment inside the shell 1. The sealing connection between the mounting tube 107 and the housing 1, as well as the sealing plug at the outer end, ensures the overall airtightness of the incubator. When no detection element is installed, the sealing plug effectively prevents gas leakage and the entry of outside air, ensuring a stable internal environment.

[0041] The sealed connection structure also prevents damage to the seal during component installation, maintaining the incubator's optimal performance. Furthermore, the design of the mounting tube 107 facilitates the replacement and maintenance of detection components. When a component malfunctions, operators can quickly replace it via the mounting tube 107 without disassembling the entire incubator structure, reducing maintenance costs.

[0042] like Figure 2 and Figure 5 As shown, the top of the housing 1 is provided with an operation box 7, and the operation box 7 is provided with a motor 701. The power output end of the motor 701 is connected to the fan 6. The operation box 7 is provided with a PLC controller, which is electrically connected to the motor 701. The PLC controller is used to control the start and stop of the motor 701.

[0043] The connection between the motor 701 and the fan 6 inside the control box 7, along with the control function of the PLC controller, enables intelligent operation of the incubator. The motor 701 provides stable power to the fan 6, ensuring the continuous operation of the air circulation system. When the air-jacketed incubator of this application is connected to an external power source, the PLC controller controls the motor 701 to drive the fan 6 to rotate, thereby achieving air circulation within the incubator. Furthermore, when the temperature inside the chamber fluctuates, the PLC controller can adjust the fan speed to speed up or slow down air circulation, quickly restoring the temperature to the set value. This improves the incubator's control precision over environmental parameters, making culture conditions more stable, which is beneficial for increasing the success rate of cell culture and the reliability of experimental results. It also enhances the automation level and ease of use of the equipment.

[0044] like Figure 3 and Figure 5 As shown, a carbon dioxide sensor is installed inside the inner liner 2, and the carbon dioxide sensor is connected to a PLC controller. A gas supply nozzle 702 is installed on the side wall of the control box 7. The outer end of the gas supply nozzle 702 is used to connect to an external carbon dioxide supply device, and the inner end of the gas supply nozzle 702 is connected to a filter 703. A solenoid valve 704 is connected to the filter 703. A carbon dioxide gas supply nozzle passes through the inner liner 2, the silicone heating layer 3, and the first insulation layer 4. The outlet end of the solenoid valve 704 is connected to the inlet end of the carbon dioxide gas supply nozzle. The solenoid valve 704 is electrically connected to the PLC controller. The PLC controller has a preset carbon dioxide concentration. The PLC controller can open or close the solenoid valve 704 according to the real-time carbon dioxide concentration monitored by the carbon dioxide sensor 202, so that the carbon dioxide concentration inside the housing 1 is not lower than the preset carbon dioxide concentration in the PLC controller. In this embodiment, the carbon dioxide gas supply nozzle is located at the top of the inner liner. In fact, the carbon dioxide gas supply nozzle can also be located on the side wall or the bottom of the inner liner 2.

[0045] The above structure constructs a fully automated carbon dioxide concentration control system, providing a precise gas environment for cell culture. A carbon dioxide sensor monitors the carbon dioxide concentration inside the inner chamber 2 in real time and transmits the data to the PLC controller. The PLC controller compares the real-time concentration with a preset concentration. When the real-time concentration is lower than the preset value, it immediately controls the solenoid valve 704 to open, drawing gas from an external carbon dioxide supply device through the gas nozzle 702. After being filtered by the filter 703, the gas is sent into the chamber to replenish carbon dioxide. When the concentration reaches the preset value, the solenoid valve 704 closes, stopping the gas replenishment. This process is fully automated, requiring no manual intervention, and ensures that the carbon dioxide concentration inside the chamber is consistently maintained within the set range with minimal error. The 703 filter effectively removes impurities and microorganisms from the supplemental gas, preventing contamination of the culture environment. Precise and stable carbon dioxide concentration is crucial for maintaining normal cellular physiological metabolism and acid-base balance, especially for primary cells and stem cells that are sensitive to gaseous environments. This precise control can significantly improve cell survival rates and growth quality, providing reliable experimental data for scientific research and medical experiments.

[0046] like Figure 4 As shown, the side wall of the control box 7 is provided with multiple heat dissipation holes 705, and the front side wall of the control box 7 is provided with an operation panel 706.

[0047] The heat dissipation holes 705 on the side wall of the control box 7 can effectively dissipate the heat generated by electronic components such as the motor 701 and PLC controller inside the control box 7 during operation. These components generate a large amount of heat during prolonged operation. If this heat cannot be dissipated in time, the temperature inside the control box 7 will rise, affecting the performance and lifespan of the components, and may even cause control system malfunctions. The presence of the heat dissipation holes 705 ensures ventilation and heat dissipation inside the control box 7, maintains the normal operating temperature of the electronic components, ensures the stable operation of the entire control system, and improves the reliability and durability of the equipment. The control panel 706 on the front wall of the control box 7 provides operators with an intuitive and convenient operating interface. Through the control panel 706, operators can easily set various parameters of the incubator and view real-time environmental parameters and equipment operating status. This design makes the equipment operation simpler and easier to understand, reduces the learning cost for operators, improves work efficiency, and facilitates the timely detection and handling of problems that occur during equipment operation.

[0048] Finally, a first sampling nozzle 707 is provided on one side of the gas supply nozzle 702. A second sampling nozzle is provided through the side wall of the inner liner 2, the silicone heating layer 3, and the first insulation layer 4. The first sampling nozzle 707 is connected to the second sampling nozzle so that a gas sample inside the inner liner can be extracted at the first nozzle.

[0049] The above are merely preferred embodiments of this application and are not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. An air-jacketed incubator, characterized in that, include: The housing (1) has an internal mounting cavity with an opening at the front end. A sealing door (101) is hinged to the housing (1) and the sealing door (101) can be flipped to open or close the mounting cavity. The inner liner (2) has an opening on its front side wall and is fixed inside the mounting cavity; A silicone heating layer (3) is wrapped around the outer wall of the inner liner (2); The first insulation layer (4) covers the outside of the silicone heating layer (3); A support frame (5) is provided inside the inner liner (2). The support frame (5) is provided with multiple culture chambers. The left side wall, right side wall and top wall of the support frame (5) are arranged at intervals with the inner wall of the inner liner (2) to form an air jacket cavity (102). The top wall, left side wall and right side wall of the support frame (5) are provided with first air holes. The fan (6) is located near the top of the inner liner (2). The fan (6) is used to draw the air in the culture chamber from the top, circulate it through the air jacket cavity (102), and then return it to the culture chamber through the side wall of the support frame (5).

2. The air-jacketed incubator as described in claim 1, characterized in that: The inner liner (2) extends outward and is provided with a fixing plate. A connecting ear (201) is connected to the fixing plate. Multiple fasteners (103) are provided on the inner wall of the shell (1). The fasteners (103) are located near the four corners of the shell (1). The connecting ear (201) is connected to the fasteners (103) by bolts.

3. The air-jacketed incubator as described in claim 2, characterized in that: The support frame (5) includes two vertically arranged air guide plates (501), a plurality of partitions (502) disposed between the air guide plates (501), and a top plate disposed between the two air guide plates (501). A cavity-forming ring (503) is provided on the outer side of the air guide plate (501), and the cavity-forming ring (503) abuts against the corresponding side wall of the inner liner (2). A sealing plate (508) is provided between the top plate (504) and the top wall of the inner liner. The inner wall of the inner liner (2), the air guide plate (501), the cavity-forming ring (503) and the sealing plate (508) form a sealed air jacket cavity (102). A plurality of second air holes (506) are uniformly provided on the partition (502).

4. The air-jacketed incubator as described in claim 3, characterized in that: From bottom to top, the density of the first pore (505) gradually decreases.

5. The air-jacketed incubator as described in claim 3, characterized in that: The air guide plate (501) has an installation hole, and a sheet metal part (507) is installed in the installation hole. The part of the sheet metal part (507) located inside the air guide plate (501) forms an insertion groove, and the partition plate (502) is inserted into the insertion groove.

6. The air-jacketed incubator as described in claim 1, characterized in that: The bottom of the housing (1) is provided with a plurality of casters (104). The casters (104) are located near the four corners of the housing (1). The mounting base (105) of the casters (104) is provided with support feet (106) that can be raised and lowered. The support feet (106) can release the casters (104) from contact with the ground by extending outward.

7. The air-jacketed incubator as described in claim 2, characterized in that: An installation tube (107) is provided through the inner liner (2), the shell (1), the first insulation layer (4) and the silicone heating layer (3). The installation tube (107) is sealed to the shell (1), and a sealing plug is provided at the outer end of the installation tube (107).

8. The air-jacketed incubator as described in claim 1, characterized in that: The top of the housing (1) is provided with an operation box (7), and the operation box (7) is provided with a motor (701). The power output end of the motor (701) is connected to the fan (6). The operation box (7) is provided with a PLC controller, which is electrically connected to the motor (701). The PLC controller is used to control the start and stop of the motor (701).

9. The air-jacketed incubator as described in claim 8, characterized in that: The inner liner (2) is equipped with a carbon dioxide sensor, which is connected to the PLC controller. The side wall of the operating box (7) is equipped with a gas supply nozzle (702). The outer end of the gas supply nozzle (702) is used to connect to an external carbon dioxide supply device. The inner end of the gas supply nozzle (702) is connected to a filter (703). A solenoid valve (704) is connected to the filter (703). A carbon dioxide gas supply nozzle is provided through the inner liner (2), the silicone heating layer (3), and the first insulation layer (4). The outlet end of the solenoid valve (704) is connected to the inlet end of the carbon dioxide gas supply nozzle. The solenoid valve (704) is electrically connected to the PLC controller. The PLC controller has a preset carbon dioxide concentration. The PLC controller can open or close the solenoid valve (704) according to the real-time carbon dioxide concentration monitored by the carbon dioxide sensor (202) so that the carbon dioxide concentration in the housing (1) is not lower than the preset carbon dioxide concentration in the PLC controller.

10. The air-jacketed incubator as described in claim 9, characterized in that: The side wall of the control box (7) is provided with a plurality of heat dissipation holes (705), and the front side wall of the control box (7) is provided with an operation panel (706).

Images