A layered brain tissue sampling device for MCAO model

Abstract

The utility model provides a kind of MCAO model brain tissue layered material taking device, comprising: pedestal.The utility model provides a kind of MCAO model brain tissue layered material taking device, and through vertical lifting mechanism, layered cutting component, sample clamping mechanism and sample collection tray mutual cooperation, the accurate layered cutting of brain tissue sample can be realized, ensure that sample thickness is uniform, position is accurate, reduce sample damage, improve sample quality, simultaneously, the vertical lifting mechanism and sample collection tray design of automation, improve the material taking efficiency, provide high-quality tissue sample for the subsequent research of MCAO model, improve the practicability and flexibility of this device.

Description

Technical Field

[0001] This utility model relates to the field of biomedical experimental equipment technology, and in particular to a brain tissue layering sampling device for an MCAO model. Background Technology

[0002] Biomedical experimental equipment is a core tool supporting life science research and medical practice, covering multi-scale research fields such as molecules, cells, and tissues. Common equipment includes high-precision microscopes (such as confocal microscopes), high-speed centrifuges, cell culture incubators, PCR instruments, and biosafety cabinets. These devices use technologies such as automated control, fluorescent labeling, and constant temperature and humidity to achieve the separation, culture, detection, and analysis of biological samples. They are widely used in disease mechanism research, drug development, gene editing, and clinical diagnosis, providing key technical support for precision medicine and translational research, and promoting the rapid development of modern biomedicine.

[0003] In MCAO model studies, layered sampling and analysis of brain tissue is an important step in exploring the mechanism of ischemic injury and treatment effects. The frozen, solidified brain tissue is placed in a sample box for cutting. Multiple blades are inserted from top to bottom into the slots of the sample box to cut the brain tissue placed in the sample box.

[0004] However, currently most cutting methods are done manually or with ordinary blades, lacking automated cutting designs.

[0005] Therefore, it is necessary to provide an MCAO model brain tissue layering sampling device to solve the above-mentioned technical problems. Utility Model Content

[0006] This invention provides a layered sampling device for brain tissue in MCAO models, which solves the problem of lack of automated cutting design in manual slicing or ordinary blade cutting methods.

[0007] To solve the above-mentioned technical problems, this utility model provides a brain tissue layering sampling device for an MCAO model, comprising: a base; A vertical lifting mechanism is fixedly installed on one side of the top of the base; A layered cutting assembly is disposed at the bottom of the movable plate. A vertical lifting mechanism is used to adjust the height of the layered cutting assembly. The layered cutting assembly includes a connecting plate, a slot, a second threaded rod, an adjusting block, a sliding plate, a threaded hole, a sliding hole, and a cutting blade. The connecting plate is disposed at the bottom of the movable plate. The slot is formed in the middle of the bottom of the connecting plate. Three second threaded rods are rotatably installed inside the slot. The adjusting block is fixedly installed at one end of the second threaded rod. Three sliding plates are threadedly engaged with the outer surfaces of the three second threaded rods. The threaded hole is formed on one side of the surface of the sliding plate. Two sliding holes are formed on both sides of the outer surface of the sliding plate. The cutting blade is fixedly installed in the middle of the bottom of the sliding plate. Two fixed blocks are respectively fixedly installed on both sides of the top of the base. A reciprocating screw is rotatably installed on the surface of the fixed block. An adjusting block is fixedly installed at one end of the reciprocating screw. Movable plates are threaded onto both sides of the outer surface of the reciprocating screw. A sample clamping mechanism is provided on the top side of the movable plate, and two sets of the sample clamping mechanism are used to restrict the sample.

[0008] Preferably, the vertical lifting mechanism includes a support plate, a slide groove, a driving component, a first threaded rod, a threaded block, and a movable plate. The support plate is fixedly installed on the top of the base. The slide groove is opened in the middle of the front side of the support plate. The driving component is fixedly installed in the middle of the top of the support plate. The first threaded rod is fixedly connected to the bottom end of the output shaft of the driving component. The threaded block is threadedly engaged with the outer surface of the first threaded rod. The movable plate is fixedly installed on one side of the outer surface of the threaded block.

[0009] Preferably, the sample clamping mechanism includes a threaded post, a threaded ring block, and a pressure plate. The threaded post is fixedly installed on one side of the top of the movable plate, the threaded ring block is threadedly engaged with the outer surface of the threaded post, and the pressure plate is rotatably installed on the bottom of the threaded ring block.

[0010] Preferably, a tray is fixedly installed on both sides of the bottom of the base, and a sample collection tray is slidably installed on the front side inside the tray. The top of the sample collection tray is divided into multiple independent sample storage areas, and each area is marked.

[0011] Preferably, a threaded groove is provided on the other side of the surface of the movable plate.

[0012] Preferably, the sample collection tray has a placement groove in the middle of the top, a partition plate is fixedly installed in the middle of the placement groove, and limit grooves are opened on both sides of the inner wall of the placement groove. A limit rod is fixedly installed inside the limit groove, and an elastic element is sleeved on one side of the outer surface of the limit rod.

[0013] Preferably, a limiting block is slidably installed on the other side of the outer surface of the limiting rod, and a limiting plate is fixedly installed on one side of the limiting block.

[0014] Compared with related technologies, the MCAO model brain tissue layering sampling device provided by this utility model has the following beneficial effects: During operation, the adjusting block is first rotated to drive the reciprocating screw to rotate. The reciprocating screw rotates and engages the threaded grooves of the two movable plates. The two movable plates slide close to or away from each other on the surface of the base. The movable plates drive the sample clamping mechanism to move, thereby adjusting the distance between the two sample clamping mechanisms, so that the two movable plates clamp the sample box placed in the middle of the top of the base from both sides. At this time, the pressure plate moves to the top of the sample box.

[0015] Rotating the threaded ring block causes the threaded ring block to engage with the threaded column, thus adjusting the height. The threaded ring block drives the pressure plate to adjust the height, allowing the pressure plate to press the sample onto the base surface. This can stably fix the sample box while avoiding excessive compression that could cause tissue damage, and can accommodate brain tissue samples of different heights.

[0016] The vertical lifting mechanism drives the first threaded rod to rotate by alternating forward and reverse rotation of the output shaft of the drive component. The rotation of the first threaded rod engages with the threaded block, which drives the moving plate to lift and lower, thereby adjusting the height of the layered cutting component and realizing the cutting action of the layered cutting component. Attached Figure Description

[0017] Figure 1 A schematic diagram of the structure of a first embodiment of an MCAO model brain tissue layering sampling device provided by this utility model; Figure 2 for Figure 1 The enlarged schematic diagram at point A is shown below; Figure 3 for Figure 2 The diagram shows the structure of the movable plate. Figure 4 for Figure 1 The diagram shows the structure of the sample clamping mechanism. Figure 5 for Figure 1 The diagram shown is a bottom view of the base. Figure 6 for Figure 5 The enlarged schematic diagram at point B is shown below; Figure 7 A schematic diagram of the structure of a second embodiment of the MCAO model brain tissue layering sampling device provided by this utility model; Figure 8 for Figure 7 The enlarged schematic diagram at point C is shown.

[0018] The following are the labeling elements in the diagram: 1. Base; 2. Vertical lifting mechanism; 21. Support plate; 22. Slide groove; 23. Drive component; 24. First threaded rod; 25. Threaded block; 26. Moving plate; 3. Layered cutting assembly; 31. Connecting plate; 32. Groove; 33. Second threaded rod; 34. Adjusting block; 35. Slide plate; 36. Threaded hole; 37. Slide hole; 38. Cutting blade; 4. Sample clamping mechanism; 41. Threaded column; 42. Threaded ring block; 43. Pressure plate; 5. Support plate; 6. Sample collection tray; 7. Movable plate; 8. Threaded groove; 9. Fixing block; 10. Reciprocating screw; 11. Adjusting block; 12. Placement groove; 13. Divider plate; 14. Limiting groove; 15. Limiting rod; 16. Elastic component; 17. Limiting block; 18. Limiting plate. Detailed Implementation

[0019] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0020] First Embodiment

[0021] Please refer to the following: Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 ,in, Figure 1 A schematic diagram of the structure of a first embodiment of an MCAO model brain tissue layering sampling device provided by this utility model; Figure 2 for Figure 1 The enlarged schematic diagram at point A is shown below; Figure 3 for Figure 2 The diagram shows the structure of the movable plate. Figure 4 for Figure 1 The diagram shows the structure of the sample clamping mechanism. Figure 5 for Figure 1 The diagram shown is a bottom view of the base. Figure 6 for Figure 5 A magnified schematic diagram of point B shown. A brain tissue layering sampling device for an MCAO model includes: a base 1; A vertical lifting mechanism 2 is fixedly installed on one side of the top of the base 1; A layered cutting assembly 3 is disposed at the bottom of the movable plate 26. The vertical lifting mechanism 2 is used to adjust the height of the layered cutting assembly 3. The layered cutting assembly 3 includes a connecting plate 31, a slot 32, a second threaded rod 33, an adjusting block 34, a sliding plate 35, a threaded hole 36, a sliding hole 37, and a cutting blade 38. The connecting plate 31 is disposed at the bottom of the movable plate 26. The slot 32 is opened in the middle of the bottom of the connecting plate 31. Three second threaded rods 33 are rotatably installed inside the slot 32. The adjusting block 34 is fixedly installed at one end of the second threaded rod 33. Three sliding plates 35 are threadedly engaged with the outer surfaces of the three second threaded rods 33. The threaded hole 36 is opened on one side of the surface of the sliding plate 35. Two sliding holes 37 are opened on both sides of the outer surface of the sliding plate 35. The cutting blade 38 is fixedly installed in the middle of the bottom of the sliding plate 35. Fixed blocks 9, two fixed blocks 9 are respectively fixedly installed on both sides of the top of the base 1. A reciprocating screw 10 is rotatably installed on the surface of the fixed block 9. An adjusting block 11 is fixedly installed at one end of the reciprocating screw 10. Movable plates 7 are threadedly engaged on both sides of the outer surface of the reciprocating screw 10. The sample clamping mechanism 4 is disposed on the top side of the movable plate 7, and two sets of the sample clamping mechanism 4 are used to restrict the sample.

[0022] The vertical lifting mechanism 2 includes a support plate 21, a slide groove 22, a driving component 23, a first threaded rod 24, a threaded block 25, and a moving plate 26. The support plate 21 is fixedly installed on the top of the base 1. The slide groove 22 is opened in the middle of the front of the support plate 21. The driving component 23 is fixedly installed in the middle of the top of the support plate 21. The first threaded rod 24 is fixedly connected to the bottom end of the output shaft of the driving component 23. The threaded block 25 is threadedly engaged with the outer surface of the first threaded rod 24. The moving plate 26 is fixedly installed on one side of the outer surface of the threaded block 24.

[0023] The sample clamping mechanism 4 includes a threaded post 41, a threaded ring block 42, and a pressure plate 43. The threaded post 41 is fixedly installed on one side of the top of the movable plate 7. The threaded ring block 42 is threadedly engaged with the outer surface of the threaded post 41. The pressure plate 43 is rotatably installed on the bottom of the threaded ring block 42.

[0024] Both sides of the base 1 are fixedly installed with trays 5. A sample collection tray 6 is slidably installed on the front side inside the tray 5. The top of the sample collection tray 6 is divided into multiple independent sample storage areas, and each area is marked.

[0025] A threaded groove 8 is provided on the other side of the surface of the movable plate 7.

[0026] Each of the three slide plates 35 has a threaded hole 36 on its surface. The threaded holes 36 on the surface of each slide plate 35 are located in different positions, so that the three slide plates 35 can be threaded onto the outer surface of the three second threaded rods 33. At the same time, the surface of each of the three slide plates 35 also has two sliding holes 37. The diameter of the sliding holes 37 is larger than the diameter of the other two second threaded rods 33, so that one threaded hole 36 on the surface of the slide plate 35 is threaded onto the outer surface of the second threaded rod 33, and the other two sliding holes 37 are fitted onto the outer surface of the other two second threaded rods 33.

[0027] The three-layer parallel arrangement of ultra-thin cutting blades 38 allows for precise adjustment of the blade spacing from 0.1 to 2 mm via the precision adjustment of the second threaded rod 33. The cutting blades 38 are made of medical-grade stainless steel, with sharp and wear-resistant cutting edges, ensuring minimal damage to brain tissue during the cutting process.

[0028] The tray 5 has a hidden groove on its side, and the sample collection tray 6 has insertion blocks on both sides. The insertion blocks can slide inside the hidden groove. A transparent label box can be set inside the sample collection tray 6. A groove is opened in the middle of one side of the label box for placing the recording label paper.

[0029] A threaded layer is formed on the inner diameter surface of the threaded ring block 42, which engages with the threaded column 41.

[0030] The drive component 23 is a servo motor.

[0031] The working principle of the MCAO model brain tissue layering sampling device provided by this utility model is as follows: During operation, firstly, rotating the adjusting block 11 drives the reciprocating screw 10 to rotate. The reciprocating screw 10 rotates and engages with the threaded grooves 8 of the two movable plates 7. The two movable plates 7 slide close to or away from each other on the surface of the base 1. The movable plates 7 drive the sample clamping mechanism 4 to move, thereby adjusting the distance between the two sample clamping mechanisms 4, so that the two movable plates 7 clamp the sample box placed in the middle of the top of the base 1 from both sides. At this time, the pressure plate 43 moves to the top of the sample box.

[0032] Rotating the threaded ring block 42 causes the threaded ring block 42 to engage with the threaded column 41, thereby adjusting the height. The threaded ring block 42 drives the pressure plate 43 to adjust the height, so that the pressure plate 43 presses the sample onto the surface of the base 1, which can stably fix the sample box and avoid excessive compression that could cause tissue damage. It can adapt to brain tissue samples of different heights.

[0033] The vertical lifting mechanism 2 drives the first threaded rod 24 to rotate by the output shaft of the drive component 23 rotating alternately in both directions. The rotation of the first threaded rod 24 engages with the threaded block 25, which drives the moving plate 26 to achieve lifting and lowering, thereby adjusting the height of the layered cutting component 3 and realizing the cutting action of the layered cutting component 3.

[0034] The layered cutting assembly 3 consists of multiple layers of parallel-arranged ultra-thin cutting blades 38. The three sets of cutting blades 38 are threadedly engaged by a slide plate 35 and a second threaded rod 33. Rotating the second threaded rod 33 can engage the slide plate 35 to adjust the position of the cutting blades 38 and precisely adjust the spacing between the blades.

[0035] The cutting blade is made of medical-grade stainless steel, with a sharp and wear-resistant edge, ensuring minimal damage to brain tissue during the cutting process. The vertical lifting mechanism 2 enables the vertical up and down movement of the layered cutting component 3. The vertical lifting mechanism 2 controls the cutting depth and speed of the layered cutting component 3 to ensure that the thickness of each tissue sample is consistent.

[0036] A pull-out sample collection tray 6 is installed below the fixed base 1. The tray is divided into multiple independent sample storage areas, each with a label, to facilitate the classification, collection and labeling of brain tissue samples from different layers by researchers.

[0037] Second Embodiment

[0038] Please refer to the following: Figure 7 and Figure 8 Based on the MCAO model brain tissue layering sampling device provided in the first embodiment of this application, the second embodiment of this application proposes another MCAO model brain tissue layering sampling device. The second embodiment is merely a preferred embodiment of the first embodiment, and the implementation of the second embodiment will not affect the separate implementation of the first embodiment.

[0039] Specifically, the difference in the MCAO model brain tissue layering sampling device provided in the second embodiment of this application is that a placement groove 12 is provided in the middle of the top of the sample collection tray 6, a partition plate 13 is fixedly installed in the middle of the placement groove 12, a limiting groove 14 is provided on both sides of the inner wall of the placement groove 12, a limiting rod 15 is fixedly installed inside the limiting groove 14, and an elastic element 16 is sleeved on one side of the outer surface of the limiting rod 15.

[0040] A limiting block 17 is slidably installed on the other side of the outer surface of the limiting rod 15, and a limiting plate 18 is fixedly installed on one side of the limiting block 17.

[0041] The elastic element 16 adopts a spring structure. One end of the elastic element 16 is connected to the limiting block 17. The restoring force of the elastic element 16 pushes the limiting block 17 to move towards the partition plate 13.

[0042] The working principle of the MCAO model brain tissue layering sampling device provided by this utility model is as follows: During operation, the limiting plate 18 is first pushed away from the partition plate 13. The limiting plate 18 causes the limiting block 17 to slide on the surface of the limiting rod 15. The limiting block 17 squeezes the elastic member 16. Then, after the sample is placed inside the placement slot 12, the limiting plate 18 is released. The restoring force of the elastic member 16 pushes the limiting plate 18 to move towards the partition plate 13. The partition plate 13 and the limiting plate 18 restrict the sample box inside the placement slot 12.

[0043] Compared with related technologies, the MCAO model brain tissue layering sampling device provided by this utility model has the following beneficial effects: This invention provides a MCAO model brain tissue layering sampling device. The limiting plate 18 is pushed by the restoring elastic force of the elastic element 16. The limiting plate 18 presses the sample against the surface of the partition plate 13, which avoids shaking when moving the sample collection tray 6 when the sample volume is not compatible with the placement slot 12, thus improving the stability of sample storage.

[0044] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A brain tissue layering sampling device for an MCAO model, characterized in that, include: Base; A vertical lifting mechanism is fixedly installed on one side of the top of the base. The vertical lifting mechanism includes a support plate, a slide groove, a driving component, a first threaded rod, a threaded block, and a movable plate. The support plate is fixedly installed on the top of the base. The slide groove is opened in the middle of the front of the support plate. The driving component is fixedly installed in the middle of the top of the support plate. The first threaded rod is fixedly connected to the bottom end of the output shaft of the driving component. The threaded block is threadedly engaged with the outer surface of the first threaded rod. The movable plate is fixedly installed on one side of the outer surface of the threaded block. A layered cutting assembly is disposed at the bottom of the movable plate. A vertical lifting mechanism is used to adjust the height of the layered cutting assembly. The layered cutting assembly includes a connecting plate, a slot, a second threaded rod, an adjusting block, a sliding plate, a threaded hole, a sliding hole, and a cutting blade. The connecting plate is disposed at the bottom of the movable plate. The slot is formed in the middle of the bottom of the connecting plate. Three second threaded rods are rotatably installed inside the slot. The adjusting block is fixedly installed at one end of the second threaded rod. Three sliding plates are threadedly engaged with the outer surfaces of the three second threaded rods. The threaded hole is formed on one side of the surface of the sliding plate. Two sliding holes are formed on both sides of the outer surface of the sliding plate. The cutting blade is fixedly installed in the middle of the bottom of the sliding plate. Two fixed blocks are respectively fixedly installed on both sides of the top of the base. A reciprocating screw is rotatably installed on the surface of the fixed block. An adjusting block is fixedly installed at one end of the reciprocating screw. Movable plates are threaded onto both sides of the outer surface of the reciprocating screw. A sample clamping mechanism is provided on one side of the top of the movable plate, and two sets of the sample clamping mechanism are used to restrict the sample; The sample clamping mechanism includes a threaded post, a threaded ring block, and a pressure plate. The threaded post is fixedly installed on one side of the top of the movable plate, the threaded ring block is threadedly engaged with the outer surface of the threaded post, and the pressure plate is rotatably installed on the bottom of the threaded ring block. Both sides of the base bottom are fixedly installed with trays, and a sample collection tray is slidably installed on the front side inside the tray. The top of the sample collection tray is divided into multiple independent sample storage areas, and each area is marked. A threaded groove is provided on the other side of the surface of the movable plate; The sample collection tray has a placement slot in the middle of the top, a partition plate is fixedly installed in the middle of the placement slot, and limit slots are opened on both sides of the inner wall of the placement slot. A limit rod is fixedly installed inside the limit slot, and an elastic element is sleeved on one side of the outer surface of the limit rod. A limit block is slidably installed on the other side of the outer surface of the limit rod, and a limit plate is fixedly installed on one side of the limit block.

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