Influenza virus detection structure
By using gold-platinum nanolabels and a pore size gradient transition layer in the influenza virus detection structure, the problems of detecting low viral load and subtype differentiation in existing technologies have been solved, achieving high sensitivity and long shelf life self-testing capability.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- HEBI COLLEGE OF VOCATION & TECH
- Filing Date
- 2025-05-20
- Publication Date
- 2026-08-04
AI Technical Summary
Existing technologies are insufficient for quickly and accurately detecting influenza samples with low viral loads, and cannot distinguish between viral subtypes. Furthermore, ordinary colloidal gold test strips have a short shelf life, and latex immunochromatographic test strips require specialized equipment, making it difficult for ordinary people to perform self-testing.
Gold-platinum nanoparticles were immobilized on the surface of nanoparticles using thiolized antibodies. The binding pad was modified with low-density silane and a pore size gradient transition layer was set. The binding pad was divided into multiple regions with gold-platinum nanoparticles set in different proportions. Combined with a hydrophobic band, the sample fluid was evenly distributed using a pore size gradient transition layer and a porous hydrophilic membrane. Specific binding and signal color development were achieved through a nitrocellulose membrane.
It improves the sensitivity and accuracy of influenza virus detection, can detect samples with low viral load, can distinguish different viral subtypes, extends the shelf life of the test strip, simplifies the testing process, and facilitates self-testing.
Smart Images
Figure CN224594654U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of virus detection technology, specifically an influenza virus detection structure. Background Technology
[0002] Every year during flu season, the number of emergency room visits increases. In order to quickly detect and triage flu patients, test strips can be used to facilitate self-testing by patients, save medical resources, and avoid cross-infection in hospitals caused by healthy people going to the hospital for testing.
[0003] Existing technologies primarily utilize colloidal gold test strips, where colloidal gold particles serve as tracer markers, combined with gold-labeled antibodies immobilized on a pad, and a nitrocellulose membrane to form a "double antibody sandwich" complex. While simple to operate, the detection limit is typically 10^3 PFU / mL, making it difficult to detect samples with low viral loads and unable to distinguish viral subtypes. Fluorescent immunochromatographic test strips offer higher sensitivity but have significant limitations, requiring specialized equipment and making self-testing difficult for the average person. Latex immunochromatographic test strips are also unsuitable for detecting samples with low viral loads. Furthermore, both colloidal gold and latex test strips have relatively short shelf lives, leading to performance degradation.
[0004] Therefore, this invention provides an influenza virus detection structure to solve the above problems. Utility Model Content
[0005] To address the shortcomings of existing technologies, this invention provides an influenza virus detection structure that solves the aforementioned problems.
[0006] To achieve the above objectives, this utility model provides the following technical solution: An influenza virus detection structure includes a shell, a sample pad, a conjugate pad, a nitrocellulose membrane, and a gold-platinum nanoparticle marker. The conjugate pad has nanoparticles adsorbed on it by electrostatic interaction. The gold-platinum nanoparticle marker is fixed to the surface of the nanoparticles by a thiolized antibody. A pore size gradient transition layer is provided between the sample pad and the nitrocellulose membrane. The conjugate pad is treated with low-density silane to encapsulate the gold-platinum nanoparticles.
[0007] Preferably, the pore size of the pore gradient transition layer decreases gradually from the sample pad to the nitrocellulose membrane, thus gradually slowing down the liquid flow rate, ensuring that the sample and the marker are fully combined, uniformly distributing the sample flow, improving signal consistency, reducing signal fluctuations in the detection line and control line, and improving detection repeatability, thereby preventing false negatives or false positives caused by uneven liquid distribution.
[0008] Preferably, the thiolized antibody is covalently fixed to the surface of the gold-platinum nanolayer, ensuring that the antibody active site faces the liquid phase, which facilitates the capture of influenza virus.
[0009] Preferably, the nitrocellulose membrane functions as a chromatography carrier in the immunochromatographic test strip, driving the flow of liquid sample through capillary action and achieving specific binding and signal development of the target analyte on its surface.
[0010] Preferably, the bonding pad is treated with low-density silane on its surface to encapsulate gold-platinum nanoparticles, preventing them from oxidizing or agglomerating during storage and extending their shelf life.
[0011] Preferably, the pore size gradient transition layer is made of polyester film.
[0012] Preferably, a fixing plate is fixedly connected inside the outer shell, and an observation port and a sample addition port are provided on the outer shell.
[0013] Preferably, an absorbent pad is fixedly connected to the fixing plate, and the sample pad, conjugate pad, and nitrocellulose membrane are fixedly connected to the fixing plate.
[0014] Preferably, the sample pad overlaps with the conjugate pad, with an overlap area of 2 mm, and the sample pad is placed on the conjugate pad. The nitrocellulose membrane overlaps with the conjugate pad, with an overlap area of 2 mm, and the conjugate pad is placed on the nitrocellulose membrane. The nitrocellulose membrane overlaps with the absorbent pad, with an overlap area of 3 mm, and the nitrocellulose membrane is placed on the absorbent pad. A porous hydrophilic membrane is provided between the sample pad and the fixing plate.
[0015] Preferably, the bonding pad is divided into a first region, a second region, a third region, and a fourth region by a hydrophobic band, and the gold-platinum nanoparticles on the first region are... Gold-platinum nanolabels on the second region Gold-platinum nanolabels on the third region Gold-platinum nanolabels on the fourth region .
[0016] Beneficial effects This invention provides a structure for detecting influenza viruses. Compared with the prior art, it has the following advantages: (1) An influenza virus detection structure in which gold-platinum nanolabels are fixed on the surface of nanoparticles by thiolized antibodies, and then the binding pads are divided into a fourth binding pad, a third binding pad, a second binding pad and a first binding pad by a hydrophobic band. Different proportions of gold-platinum nanolabels are set in the four regions to distinguish different influenza viruses. The gold-platinum nanoparticles set have high sensitivity and are easy to detect samples with low viral load.
[0017] (2) An influenza virus detection structure, which uses low-density silane to modify the surface of the binding pad and encapsulate gold-platinum nanoparticles, thereby chemically protecting the nanoparticles from oxidation and maintaining the long-term stability of the test strip, thus extending the storage time and maintaining long-term stable performance. Attached Figure Description
[0018] Figure 1 This is a side view of the overall device structure of this utility model; Figure 2 This is a diagram showing the internal structure of the overall device of this utility model; Figure 3 This is a side view of the bonding pad of this utility model; Figure 4 This is the utility model Figure 2 Partial A structure diagram; Figure 5 This is the utility model Figure 2 Local B-structure diagram.
[0019] In the diagram: 1. Outer shell; 2. Observation port; 3. Sample dispensing port; 4. Fixing plate; 5. Sample pad; 6. Binding pad; 61. First region; 62. Second region; 63. Third region; 64. Fourth region; 65. Hydrophobic zone; 7. Porous hydrophilic membrane; 8. Nitrocellulose membrane; 9. Absorbent pad; 10. Pore size gradient transition layer; Detailed Implementation
[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0021] Example 1: Please see Figure 1-5 An influenza virus detection structure includes a shell 1, a sample pad 5, a conjugate pad 6, a nitrocellulose membrane 8, and gold-platinum nanoparticles. Nanoparticles are adsorbed on the conjugate pad 6 by electrostatic interaction. The gold-platinum nanoparticles are fixed to the surface of the nanoparticles by thiolized antibodies. A pore size gradient transition layer 10 is provided between the sample pad 5 and the nitrocellulose membrane 8. The conjugate pad 6 is treated with low-density silane to encapsulate the gold-platinum nanoparticles.
[0022] The pore size gradient transition layer 10 is made of polyester film.
[0023] The outer shell 1 is fixedly connected to a fixing plate 4, and the outer shell 1 is provided with an observation port 2 and a sample addition port 3.
[0024] An absorbent pad 9 is fixedly connected to the fixing plate 4, and a sample pad 5, a conjugation pad 6, and a nitrocellulose membrane 8 are fixedly connected to the fixing plate 4.
[0025] The sample pad 5 overlaps with the conjugate pad 6, with an overlap area of 2 mm. The sample pad 5 is placed on the conjugate pad 6. The nitrocellulose membrane 8 overlaps with the conjugate pad 6, with an overlap area of 2 mm. The conjugate pad 6 is placed on the nitrocellulose membrane 8. The nitrocellulose membrane 8 overlaps with the absorbent pad 9, with an overlap area of 3 mm. The nitrocellulose membrane 8 is placed on the absorbent pad 9. A porous hydrophilic membrane 7 is provided between the sample pad 5 and the fixing plate 4.
[0026] The bonding pad 6 is divided into a first region 61, a second region 62, a third region 63, and a fourth region 64 by a hydrophobic band 65. The gold-platinum nanoparticles on the first region 61 are... Gold-platinum nanolabels on region 62 Gold-platinum nanolabels on region 63 Gold-platinum nanolabels on region 64 .
[0027] Working process: Using tools, samples such as pharyngeal swabs and nasal secretions are applied to the sample pad 5 through the sample application port 3. The samples migrate from the sample pad 5 to the conjugate pad 6 through capillary action and then pass through the porous hydrophilic membrane 7. The liquid migration speed in sample pad 5 may be uneven due to sample viscosity or uneven sample volume, resulting in unevenness of the first binding pad 61, the second binding pad 62, the third binding pad 63, and the fourth binding pad 64 on the binding pad 6. The sample fluid is evenly dispersed through the capillary network of the porous hydrophilic membrane 7 by the pore size sieving effect, avoiding local liquid flow that is too fast or too slow, reducing the surface tension of the sample, promoting stable liquid diffusion, and avoiding uneven sample volume.
[0028] Influenza virus-specific antibodies are immobilized on the surface of gold-platinum nanoparticles via chemical coupling, making the gold-platinum nanoparticles labeled with antibodies a visible probe. When the complex flows through the detection line on the nitrocellulose membrane 8 with the liquid, a large number of gold-platinum nanoparticles are enriched at the T line. Other antigenic epitopes on the virus surface will undergo a double antibody sandwich reaction with the second antibody immobilized at the T line, forming an "antibody-virus-antibody-gold-platinum nanoparticle" structure. A large number of gold-platinum nanoparticles are enriched at the T line, showing a colored band due to the SPR effect. The gold-platinum nanoparticle-antibody complex that has not bound to the virus continues to migrate to the C line and binds to the anti-antibody immobilized at the C line, forming an "antibody-nanoparticle-antibody" structure, which also shows a colored band due to the enrichment of nanoparticles.
[0029] For patients with influenza A (H1N1), the T band appears red; for influenza A (H3N2), the T band appears purple; for influenza B antigen, the T band appears blue; and for common influenza NP, the T band appears orange.
[0030] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.
[0031] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.
[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A structure for detecting influenza virus, characterized in that, The sample pad includes a shell (1), a sample pad (5), a conjugate pad (6), a nitrocellulose membrane (8), and gold-platinum nanoparticles. Nanoparticles are adsorbed on the conjugate pad (6) by electrostatic interaction. The gold-platinum nanoparticles are fixed on the surface of the nanoparticles by thiolized antibodies. A pore size gradient transition layer (10) is provided between the sample pad (5) and the nitrocellulose membrane (8). The conjugate pad (6) is treated with low-density silane on its surface to encapsulate gold-platinum nanoparticles.
2. The influenza virus detection structure according to claim 1, characterized in that: The pore size gradient transition layer (10) is made of polyester film.
3. The influenza virus detection structure according to claim 1, characterized in that: The outer shell (1) is fixedly connected to a fixing plate (4), and the outer shell (1) is provided with an observation port (2) and a sample addition port (3).
4. The influenza virus detection structure according to claim 3, characterized in that: An absorbent pad (9) is fixedly connected to the fixing plate (4), and the sample pad (5), conjugate pad (6) and nitrocellulose membrane (8) are fixedly connected to the fixing plate (4).
5. The influenza virus detection structure according to claim 4, characterized in that: The sample pad (5) overlaps with the conjugate pad (6) with an overlap area of 2 mm, and the sample pad (5) is placed on the conjugate pad (6). The nitrocellulose membrane (8) overlaps with the conjugate pad (6) with an overlap area of 2 mm, and the conjugate pad (6) is placed on the nitrocellulose membrane (8). The nitrocellulose membrane (8) overlaps with the absorbent pad (9) with an overlap area of 3 mm, and the nitrocellulose membrane (8) is placed on the absorbent pad (9). A porous hydrophilic membrane (7) is provided between the sample pad (5) and the fixing plate (4).
6. The influenza virus detection structure according to claim 5, characterized in that: The bonding pad (6) is divided into a first region (61), a second region (62), a third region (63), and a fourth region (64) by a hydrophobic band (65). The gold-platinum nanolabeled material on the first region (61) is... Gold-platinum nanolabels on the second region (62) Gold-platinum nanolabels on the third region (63) Gold-platinum nanolabels on the fourth region (64) .