Dynamic cultivation of microorganisms multiphase flow saturation and capillary pressure measuring device

Abstract

The utility model discloses a kind of dynamic culture microorganism multiphase flow saturation and capillary pressure measuring device, including experimental box, nutrient solution conveying component, non-wetting phase box and glass tube, the multiple perforated plate and water-permeable stone of being sequentially spaced distribution along up-down direction are equipped in experimental box upper end, experimental box is opened with at least one liquid inlet, first liquid outlet and second liquid outlet, liquid inlet is set above corresponding porous medium sample, first liquid outlet is set between corresponding multiple perforated plate and water-permeable stone, second liquid outlet is set below corresponding water-permeable stone;Nutrient solution conveying component includes nutrient solution tank, first overflow tank and second overflow tank, first overflow tank height adjustable, with nutrient solution tank, liquid inlet connection, second overflow tank height adjustable, with first liquid outlet connection;Non-wetting phase box is connected with liquid inlet;Glass tube is connected with second liquid outlet, and height adjustable, is equipped with scale line;Establish the dynamic change relationship of capillary pressure and saturation under different microorganism amount in multiphase flow.

Description

Technical Field

[0001] This utility model relates to the field of fluid dynamics parameter measurement technology, specifically to a device for measuring the saturation and capillary pressure of multiphase flow in dynamic culture of microorganisms. Background Technology

[0002] With the development of society and economy, groundwater polluted by the petroleum and chemical industries is mostly multiphase flow that is extremely difficult to treat. Saturation and capillary pressure are extremely important dynamic parameters in multiphase flow. The relationship between saturation and capillary pressure in porous media multiphase flow is an important relationship in oilfield development and groundwater pollution treatment.

[0003] Currently, the relationship between multiphase flow saturation and capillary pressure in porous media has been explored in various aspects, both domestically and internationally, such as the type of porous media sample, the mathematical model used, and the interphase interface area. However, most studies have neglected the influence of microorganisms on the relationship between the two. Utility Model Content

[0004] The main purpose of this invention is to provide a device for measuring the saturation and capillary pressure of multiphase flow in dynamic microbial culture, in order to solve the above-mentioned problems.

[0005] To achieve the above objectives, this utility model proposes a device for measuring the saturation and capillary pressure of a dynamic cultured microorganism multiphase flow, comprising:

[0006] The experimental chamber has a first opening at its upper end for injecting microbial inoculum. The experimental chamber contains a porous plate and a permeable stone, which are arranged alternately in the vertical direction. A porous medium sample is placed on the porous plate. The experimental chamber has at least one inlet, a first outlet, and a second outlet. The inlet is located above the porous medium sample, the first outlet is located between the porous plate and the permeable stone, and the second outlet is located below the permeable stone.

[0007] A nutrient solution delivery assembly includes a nutrient solution tank, a first overflow tank, and a second overflow tank. The nutrient solution tank is used to contain nutrient solution. The height of the first overflow tank is adjustable in the vertical direction so that it can be set higher than the porous media sample. The first overflow tank is connected to the nutrient solution tank and the inlet to input nutrient solution into the experimental chamber. The height of the second overflow tank is adjustable in the vertical direction so that it can be set flush with the porous media sample. The second overflow tank is connected to the first outlet.

[0008] A non-wetting phase chamber, containing a non-wetting phase fluid, and connected to the inlet to inject the non-wetting phase fluid into the experimental chamber; and,

[0009] A glass tube with a second opening at its upper end is connected to a second liquid outlet and its height is adjustable in the vertical direction to generate negative pressure in the experimental chamber to drive the non-wetting phase fluid to penetrate into the porous medium sample and receive the mixed liquid of microbial inoculation solution and nutrient solution flowing out of the porous medium sample through the permeable stone. The glass tube is provided with graduation lines.

[0010] Optionally, the first overflow tank is provided with a first baffle extending in the vertical direction. The bottom end of the first baffle abuts against the bottom wall of the first overflow tank, and a first gap is provided between the top end of the first baffle and the top end of the first overflow tank to divide the first overflow tank into a connected infusion tank and a return tank.

[0011] The infusion tank is connected to the nutrient solution tank via a first water pipe and to the inlet via a second water pipe. The return tank is connected to the nutrient solution tank via a third water pipe.

[0012] Optionally, a peristaltic pump is provided on the first water pipe;

[0013] The second water pipe is equipped with a first valve.

[0014] Optionally, the liquid inlet is provided, and the non-wetting phase tank is connected to the second water pipe through a fourth water pipe.

[0015] Optionally, a second valve is provided on the fourth water pipe.

[0016] Optionally, the second overflow tank is provided with a second baffle extending in the vertical direction. The bottom end of the second baffle abuts against the bottom wall of the second overflow tank, and a second gap is provided between the top end of the second baffle and the top end of the second overflow tank to divide the second overflow tank into a first recycling tank and a second recycling tank that are connected.

[0017] The first recovery tank is connected to the first liquid outlet via a fifth water pipe, and a third valve is provided on the fifth water pipe.

[0018] Optionally, a support plate is provided above the bottom of the experimental chamber, the support plate is hollowed out, and the permeable stone is placed on the support plate;

[0019] The permeable stone is provided with multiple support columns to support the perforated plate.

[0020] Optionally, the experimental chamber includes:

[0021] The main body of the tank is cylindrical and extends vertically. The perforated plate and the permeable stone are disposed inside the main body of the tank. The first opening, the liquid inlet, and the first liquid outlet are located within the main body of the tank.

[0022] The liquid collection section is located at the lower end of the main body of the tank and is connected to the main body of the tank. The liquid collection section is funnel-shaped and its diameter gradually decreases along the vertical direction. The second liquid outlet is located at the bottom end of the liquid collection section.

[0023] The technical solution of this utility model considers the influence of microbial factors on the relationship between saturation and capillary pressure. By setting up the nutrient solution tank, the first overflow tank, and the second overflow tank to continuously input nutrient solution into the experimental chamber, microorganisms are continuously cultured. Then, by adjusting the height of the glass tube to generate negative pressure, the multiphase flow in the experimental chamber is dynamically balanced, and parameters are measured in real time to achieve continuous monitoring of the dynamic growth process of microorganisms. This establishes the dynamic relationship between capillary pressure and saturation under different microbial quantities in the multiphase flow, thereby capturing the influence of the microbial quantity on the multiphase flow parameters during continuous changes over time. This is closer to the evolution process of microorganisms in the actual environment, which is consistent with the actual situation of multiphase flow treatment. It has broad applicability for the treatment of industrial polluted groundwater under natural conditions and the development of new bioremediation technologies. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0025] Figure 1 A schematic diagram of an embodiment of the device for measuring the saturation and capillary pressure of dynamic cultured microorganisms provided by this utility model;

[0026] Figure 2 A flowchart of the method for determining the relationship between saturation and capillary pressure in multiphase flow of porous media provided by this utility model;

[0027] Figure 3 This is a graph showing the dynamic relationship between capillary pressure and saturation under different microbial biomass conditions in multiphase flow, established based on the results of multiple experimental measurements.

[0028] Explanation of icon numbers:

[0029]

[0030] The realization of the purpose, functional features and advantages of this utility model will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] It should be noted that if the embodiments of this utility model involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.

[0033] Furthermore, if the embodiments of this utility model involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0034] With the development of society and economy, especially the increase in the petrochemical industry, groundwater pollution has become increasingly serious, threatening the safety of drinking water and the health of the people. At present, groundwater polluted by the petroleum and chemical industries is mostly multiphase flow, which is extremely difficult to treat. Saturation and capillary pressure are extremely important dynamic parameters in multiphase flow. The relationship between saturation and capillary pressure in porous media multiphase flow is an important relationship in oilfield development and groundwater pollution treatment.

[0035] Currently, the relationship between multiphase flow saturation and capillary pressure in porous media has been explored in various aspects, both domestically and internationally, such as the types of porous media, the mathematical models used, and the interphase interface area. However, most studies have neglected the influence of microorganisms on this relationship.

[0036] In view of this, the present invention provides a device 100 for measuring the saturation and capillary pressure of a dynamic cultured microorganism multiphase flow. Figure 1 An embodiment of the device 100 for measuring the saturation and capillary pressure of dynamic cultured microorganisms provided by this utility model.

[0037] Please see Figure 1 The dynamic microbial multiphase flow saturation and capillary pressure measuring device 100 includes an experimental chamber 1, a nutrient solution delivery assembly 2, a non-wetting phase chamber 3, and a glass tube 4. The experimental chamber 1 has a first opening at its upper end for injecting microbial inoculum. The experimental chamber 1 contains a porous plate 5 and a permeable stone 6, which are spaced apart vertically. A porous medium sample 200 is placed on the porous plate 5. The experimental chamber 1 has at least one inlet, a first outlet, and a second outlet. The inlet is positioned above the porous medium sample 200, the first outlet is positioned between the porous plate 5 and the permeable stone 6, and the second outlet is positioned below the permeable stone 6. The nutrient solution delivery assembly 2 includes a nutrient solution tank 21, a first overflow tank 22, and a second overflow tank 23. The nutrient solution tank 21 holds the nutrient solution, and the first overflow tank 22 is positioned vertically... The height of the first overflow tank 22 is adjustable so that it can be set higher than the porous medium sample 200. The first overflow tank 22 is connected to the nutrient solution tank 21 and the inlet to input nutrient solution into the experimental chamber 1. The height of the second overflow tank 23 is adjustable in the vertical direction so that it can be set flush with the porous medium sample 200. The second overflow tank 23 is connected to the first outlet. The non-wetting phase tank 3 contains non-wetting phase fluid and is connected to the inlet to inject the non-wetting phase fluid into the experimental chamber 1. The upper end of the glass tube 4 has a second opening. The glass tube 4 is connected to the second outlet and is adjustable in the vertical direction to generate negative pressure in the experimental chamber 1 to drive the non-wetting phase fluid to penetrate into the porous medium sample 200. It also receives the microbial inoculation solution and nutrient solution flowing out of the porous medium sample 200 and passing through the permeable stone 6. The glass tube 4 is provided with graduation lines.

[0038] In this utility model, the influence of microbial factors on the relationship between saturation and capillary pressure is considered. By continuously inputting nutrient solution into the experimental chamber 1 through the nutrient solution tank 21, the first overflow tank 22, and the second overflow tank 23, microorganisms are continuously cultured. Then, by adjusting the height of the glass tube 4, negative pressure is generated to drive the dynamic equilibrium of multiphase flow within the experimental chamber 1, and parameters are measured in real time to achieve continuous monitoring of the dynamic growth process of microorganisms. This establishes the dynamic relationship between capillary pressure and saturation under different microbial quantities in multiphase flow, thereby capturing the influence of continuous changes in microbial quantity over time on multiphase flow parameters. This more closely reflects the evolutionary process of microorganisms in the actual environment, thus conforming to the actual situation of multiphase flow treatment. It has broad applicability for the treatment of industrial polluted groundwater under natural conditions and the development of new bioremediation technologies.

[0039] It should be noted that in this invention, the porous media sample 200 refers to a solid containing a large number of pores, that is, a medium containing various types of capillary systems such as pores and microcracks in a solid material. Multiphase flow of porous media refers to a process in which the porous media sample 200 is the solid phase, and other phases are a mixture of nutrient solution, a non-wetting fluid, and a microbial inoculum liquid, wherein the nutrient solution and the microbial inoculum liquid are the wetting fluids. More specifically, in one embodiment of this invention, the non-wetting fluid is LNAPL (light non-aqueous liquid), that is, an oil phase with a density less than water.

[0040] It should also be noted that in this invention, the permeable stone 6 only allows the liquid phase to pass through within its critical negative pressure range.

[0041] It should also be noted that, in this utility model, the experimental chamber 1 is acid-resistant, and more specifically, the experimental chamber 1 is made of acrylic plastic.

[0042] Further, please refer to Figure 1 The first overflow tank 22 is equipped with a first baffle 221 extending vertically. The bottom end of the first baffle 221 abuts against the bottom wall of the first overflow tank 22, and a first gap is provided between the top end of the first baffle 221 and the top end of the first overflow tank 22, dividing the first overflow tank 22 into a connected infusion tank 222 and a return tank 223. The infusion tank 222 is connected to the nutrient solution tank 21 via a first water pipe 24 and to the inlet via a second water pipe 25. The return tank 223 is connected to the nutrient solution tank 21 via a third water pipe 26. Thus, the infusion tank 222 and the return tank 223 are connected through the first gap. When the liquid level of the nutrient solution injected into the infusion tank 222 from the nutrient solution tank 21 is greater than the height of the first baffle 221, it will flow into the return tank 223, thereby ensuring a stable water head height, that is, ensuring stable water pressure in the infusion tank 222.

[0043] It should be noted that the flow direction of the nutrient solution in the nutrient solution tank 21 is as follows: the nutrient solution in the nutrient solution tank 21 first flows into the infusion tank 222 through the first water pipe 24. When the liquid level of the nutrient solution in the infusion tank 222 is lower than the height of the first baffle 221, all the nutrient solution in the infusion tank 222 flows into the experimental chamber 1 through the second water pipe 25 and the inlet. When the liquid level of the nutrient solution in the infusion tank 222 is higher than the height of the first baffle 221, the nutrient solution above the first baffle 221 flows through the first gap into the return tank 223, and then flows back into the nutrient solution tank 21 through the third water pipe 26 to continue supplying nutrient solution to the infusion tank 222.

[0044] Further, please refer to Figure 1 The first water pipe 24 is equipped with a peristaltic pump 27 to continuously pump the nutrient solution in the nutrient solution tank 21 into the experimental chamber 1. The second water pipe 25 is equipped with a first valve 28, which can be used to control the input of nutrient solution, that is, to control whether nutrient solution is input into the experimental chamber 1 and to control the input rate of nutrient solution to meet experimental requirements.

[0045] Specifically, in this utility model, there can be two or one liquid inlet; when there are two liquid inlets, the infusion tank 222 and the non-wetting phase box 3 are connected to the two liquid inlets one-to-one; and when there is only one liquid inlet, please refer to... Figure 1 The non-wetting phase chamber 3 is connected to the second water pipe 25 via a fourth water pipe 29; thus, the non-wetting phase fluid and the nutrient solution enter the experimental chamber 1 through the same inlet, simplifying the structure. More specifically, the fourth water pipe 29 and the second water pipe 25 are connected via a three-way valve.

[0046] Further, please refer to Figure 1 The fourth water pipe 29 is equipped with a second valve 2a; thus, the input of the non-wetting phase fluid can be controlled, that is, whether the non-wetting phase fluid is input into the experimental chamber 1 and the input rate of the non-wetting phase fluid can be controlled to meet the experimental requirements.

[0047] For details, please refer to Figure 1 The second overflow tank 23 is equipped with a second baffle 231 extending vertically. The bottom end of the second baffle 231 abuts against the bottom wall of the second overflow tank 23, and a second gap is provided between the top end of the second baffle 231 and the top end of the second overflow tank 23, dividing the second overflow tank 23 into a first recovery tank 232 and a second recovery tank 233 that are connected. The first recovery tank 232 is connected to the first outlet through a fifth water pipe 2b, and a third valve 2c is provided on the fifth water pipe 2b. Thus, the first recovery tank 232 and the second recovery tank 233 are connected through the second gap. When the liquid level of the wet phase fluid (i.e., microbial inoculum and nutrient solution) flowing into the first recovery tank 232 is greater than the height of the second baffle 231, it will flow into the second recovery tank 233, thereby ensuring a stable head height, that is, ensuring stable water pressure in the first recovery tank 232. In addition, the third valve 2c controls the output of the mixed liquid of microbial inoculum and nutrient solution in the experimental chamber 1 to the first recovery tank 232.

[0048] It should be noted that the second recycling tank 233 is connected to the external water collection tank.

[0049] For details, please refer to Figure 1 The experimental chamber 1 has a support plate at the bottom and above, the support plate is hollowed out, and the permeable stone 6 is placed on the support plate; the permeable stone 6 has a plurality of support columns 7 to support the perforated plate 5.

[0050] For details, please refer to Figure 1 The experimental chamber 1 includes a main body and a liquid collection section. The main body is cylindrical and extends through the chamber in the vertical direction. The porous plate 5 and the permeable stone 6 are located inside the main body 11. The first opening, the liquid inlet, and the first liquid outlet are located in the main body 11. The liquid collection section is located at the lower end of the main body and communicates with it. The liquid collection section is funnel-shaped and its diameter gradually decreases in the vertical direction. The second liquid outlet is located at the bottom end of the liquid collection section.

[0051] Specifically, in this invention, the method for adjusting the height of the first overflow tank 22, the second overflow tank 23, and the glass tube 4 in the vertical direction is not limited. Specifically, in one embodiment of this invention, the first overflow tank 22, the second overflow tank 23, and the glass tube 4 are each slidably mounted on a bracket and can move vertically to achieve height adjustment. Of course, in another embodiment of this invention, the first overflow tank 22, the second overflow tank 23, and the glass tube 4 are detachably connected to one of a plurality of mounting parts on a bracket, such as by threaded connection, snap-fit, etc., so that the height of the first overflow tank 22, the second overflow tank 23, and the glass tube 4 is adjustable.

[0052] This invention also provides a method for determining the saturation and capillary pressure of multiphase flow in dynamic microbial culture, applicable to the aforementioned device for determining the saturation and capillary pressure of multiphase flow in dynamic microbial culture. Please refer to [link to relevant documentation]. Figure 2 The method for determining the saturation and capillary pressure of multiphase flow in dynamic microbial culture includes the following steps:

[0053] Step S100: Place the porous medium sample on the porous plate and inject the microbial inoculation solution into the experimental chamber through the first opening of the experimental chamber.

[0054] Step S200: Adjust the height of the glass tube to drive the microbial inoculation solution to penetrate into the porous medium sample until the porous medium sample is saturated, and adjust the 0 mark of the glass tube to be flush with the upper surface of the porous medium sample.

[0055] In this step, after the height of the glass tube is adjusted, the measuring device is left to stand for 2 days.

[0056] Step S300: Adjust the height of the first overflow tank so that it is above the porous medium sample, and adjust the height of the second overflow tank so that it is flush with the porous medium sample. Then, continuously input nutrient solution into the experimental chamber through the liquid inlet of the experimental chamber according to the preset microbial culture time.

[0057] In this step, the heights of the first and second overflow tanks are first adjusted, and then the peristaltic pump, the first valve, and the third valve are opened to continuously inject nutrient solution into the experimental chamber to cultivate microorganisms.

[0058] Step S400: Stop inputting the nutrient solution and input a non-wetting phase fluid into the experimental chamber through the inlet of the experimental chamber.

[0059] In this step, the first valve is closed to stop the input of the nutrient solution. After the liquid level in the experimental chamber stabilizes, the third valve is closed and the second valve is opened to input the non-wetting phase fluid into the experimental chamber.

[0060] Step S500: Stop inputting the non-wetting phase fluid and move the glass tube downwards to generate negative pressure in the experimental chamber, driving the non-wetting phase fluid to penetrate into the porous medium sample and driving the microbial inoculation solution and nutrient solution in the porous medium sample to flow out, so that the microbial inoculation solution and nutrient solution flow through the permeable stone and into the glass tube through the second outlet of the experimental chamber.

[0061] In this step, the second valve is first closed to stop the input of the non-wetting phase fluid. Then, the glass tube is moved downward, and a negative pressure is generated inside the experimental chamber. This causes the non-wetting phase fluid on the upper side of the porous medium sample to penetrate into the porous medium sample, thereby displacing the microbial inoculation solution and nutrient solution (i.e., the wetting phase fluid) inside the porous medium sample. The wetting phase fluid then flows out of the porous medium sample and through the permeable stone into the glass tube via the second outlet.

[0062] Step S600: When the liquid level change value in the glass tube is within the preset range, the saturation of the wetted phase fluid, the saturation of the non-wetted phase fluid, and the magnitude of the capillary pressure in the porous medium sample are measured, and a portion of the porous medium sample is taken out from the experimental chamber to measure the amount of microorganisms inside.

[0063] In this step, the liquid level height, i.e., the liquid volume, inside the glass tube is obtained through the scale lines on the glass tube, thereby obtaining the saturation of the wetted phase fluid. Then, the saturation of the non-wetting phase fluid is calculated based on the fact that the sum of the saturation of the wetted phase fluid and the saturation of the non-wetting phase fluid is 1. At the same time, the height difference between the liquid level inside the glass tube and the top surface of the porous medium sample is measured using a ruler, thereby obtaining the magnitude of the capillary pressure. Furthermore, based on the measurement results, the dynamic relationship between capillary pressure and saturation in multiphase flow is established.

[0064] In addition, in one embodiment of this utility model, the cation exchange resin method is used to extract EPS (extracellular polymeric substances) and determine the quality of microorganisms. The cation exchange resin method is existing technology and will not be described in detail here.

[0065] It should be noted that when the liquid level change in the glass tube is within the preset range, the liquid level in the glass tube tends to remain constant, that is, the change in the saturation of the wetted phase fluid is very small. At this time, the experiment is stopped, and the saturation and capillary pressure are measured.

[0066] Step S700: Clean the experimental chamber and adjust the preset microbial culture time. Repeat steps S100-S600 above.

[0067] In this step, the experimental chamber is cleaned by removing the porous media sample that has already been tested and replacing it with a new porous media sample. The preset microbial culture time is adjusted; that is, as the number of experiments increases, the preset microbial culture time is also extended, thus resulting in different microbial quantities in each experiment. More specifically, the interval between extensions is one day. For example, the preset microbial culture time is one day for the first experiment, two days for the second, three days for the third, and so on. Furthermore, based on the results of multiple measurements, a dynamic relationship between capillary pressure and saturation under different microbial quantities in multiphase flow is established (e.g., ...). Figure 3 (As shown).

[0068] The above description is only a preferred embodiment of the present utility model and does not limit the patent scope of the present utility model. All equivalent structural transformations made under the inventive concept of the present utility model using the contents of the present utility model specification and drawings, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present utility model.

Claims

1. A device for measuring the saturation and capillary pressure of a dynamic multiphase flow for culturing microorganisms, characterized in that, The device for measuring the saturation and capillary pressure of the dynamic cultured microorganism multiphase flow includes: The experimental chamber has a first opening at its upper end for injecting microbial inoculum. The experimental chamber contains a porous plate and a permeable stone, which are arranged alternately in the vertical direction. A porous medium sample is placed on the porous plate. The experimental chamber has at least one inlet, a first outlet, and a second outlet. The inlet is located above the porous medium sample, the first outlet is located between the porous plate and the permeable stone, and the second outlet is located below the permeable stone. A nutrient solution delivery assembly includes a nutrient solution tank, a first overflow tank, and a second overflow tank. The nutrient solution tank is used to contain nutrient solution. The height of the first overflow tank is adjustable in the vertical direction so that it can be set higher than the porous media sample. The first overflow tank is connected to the nutrient solution tank and the inlet to input nutrient solution into the experimental chamber. The height of the second overflow tank is adjustable in the vertical direction so that it can be set flush with the porous media sample. The second overflow tank is connected to the first outlet. A non-wetting phase chamber, containing a non-wetting phase fluid, and connected to the inlet to inject the non-wetting phase fluid into the experimental chamber; and, A glass tube with a second opening at its upper end is connected to a second liquid outlet and its height is adjustable in the vertical direction to generate negative pressure in the experimental chamber to drive the non-wetting phase fluid to penetrate into the porous medium sample and receive the microbial inoculation solution and nutrient solution flowing out of the porous medium sample and permeating through the permeable stone. The glass tube is provided with graduation lines.

2. The device for measuring the saturation and capillary pressure of dynamic cultured microorganisms in multiphase flow as described in claim 1, characterized in that, The first overflow tank is provided with a first baffle extending in the vertical direction. The bottom end of the first baffle abuts against the bottom wall of the first overflow tank, and a first gap is provided between the top end of the first baffle and the top end of the first overflow tank to divide the first overflow tank into a connected infusion tank and a return tank. The infusion tank is connected to the nutrient solution tank via a first water pipe and to the inlet via a second water pipe. The return tank is connected to the nutrient solution tank via a third water pipe.

3. The device for measuring the saturation and capillary pressure of dynamic cultured microorganisms in multiphase flow as described in claim 2, characterized in that, A peristaltic pump is installed on the first water pipe; The second water pipe is equipped with a first valve.

4. The device for measuring the saturation and capillary pressure of dynamic cultured microorganisms in multiphase flow as described in claim 2, characterized in that, The liquid inlet is provided, and the non-wetting phase tank is connected to the second water pipe through the fourth water pipe.

5. The device for measuring the saturation and capillary pressure of dynamic cultured microorganisms in multiphase flow as described in claim 4, characterized in that, The fourth water pipe is equipped with a second valve.

6. The device for measuring the saturation and capillary pressure of dynamic cultured microorganisms in multiphase flow as described in claim 1, characterized in that, The second overflow tank is provided with a second baffle extending in the vertical direction. The bottom end of the second baffle abuts against the bottom wall of the second overflow tank, and a second gap is provided between the top end of the second baffle and the top end of the second overflow tank to divide the second overflow tank into a first recycling tank and a second recycling tank that are connected. The first recovery tank is connected to the first liquid outlet via a fifth water pipe, and a third valve is provided on the fifth water pipe.

7. The device for measuring the saturation and capillary pressure of dynamic cultured microorganisms in multiphase flow as described in claim 1, characterized in that, A support plate is provided above the bottom of the experimental box. The support plate is hollowed out, and the permeable stone is placed on the support plate. The permeable stone is provided with multiple support columns to support the perforated plate.

8. The device for measuring the saturation and capillary pressure of dynamic cultured microorganisms in multiphase flow as described in claim 1, characterized in that, The experimental chamber includes: The main body of the tank is cylindrical and extends vertically. The perforated plate and the permeable stone are disposed inside the main body of the tank. The first opening, the liquid inlet, and the first liquid outlet are located within the main body of the tank. The liquid collection section is located at the lower end of the main body of the tank and is connected to the main body of the tank. The liquid collection section is funnel-shaped and its diameter gradually decreases along the vertical direction. The second liquid outlet is located at the bottom end of the liquid collection section.

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