Nanogold brucellosis detection device

Abstract

The utility model discloses a nanometer gold brucellosis detection device, including the protection box, still including the first groove body and detection component, the first groove body is passed through and is set to the left end of protection box, and the storage box is slidably arranged to the inner wall of first groove body, and the bottom of storage box inner wall is slidably arranged with two clamping plates, and the spring is fixedly connected between the clamping plate and the inner wall of storage box, and the detection box is arranged between the two clamping plates, and the top of protection box inner wall is fixedly connected with two symmetrical ultraviolet disinfection lamp, and the inner wall of protection box is provided with the light reflection coating, and the detection component is arranged in the detection box, the utility model discloses by pulling two clamping plates outward, detection box is put into the clamping plate between, and the spring pushes the clamping plate and clamps the detection box, and then storage box is pushed into the protection box, avoids the interference of external factor to the detection result, and the inside of protection box is disinfected through the opening ultraviolet disinfection lamp, and the reflection of ultraviolet is enhanced by light reflection coating, and the disinfection effect is improved, and the influence of the residual bacteria on the next detection result is avoided.

Description

Technical Field

[0001] This utility model relates to the field of bacterial disease detection devices, specifically to a nano-gold brucellosis detection device. Background Technology

[0002] Brucellosis is a zoonotic infectious disease caused by Brucella bacteria. It is highly contagious and pathogenic, posing a serious threat to livestock development and human public health. Brucella can be transmitted through contact with the secretions of infected animals, consumption of contaminated meat and dairy products, or inhalation of bacterial aerosols. Infection can lead to symptoms such as fever, joint pain, and hepatosplenomegaly. In severe cases, it can cause chronic infection and lifelong immune damage.

[0003] Existing brucellosis detection devices work by adding a sample to a test card. If Brucella is present, it will combine with two antibodies to form an "antibody-antigen-nano gold antibody" complex, which accumulates on the test line, causing a local red band. By observing the presence and intensity of the band, a qualitative or semi-quantitative judgment of brucellosis infection can be made.

[0004] Traditional testing devices mostly use open-type test strips, which are easily affected by external environmental interference. In outdoor settings such as farms, there are large temperature differences between day and night. Such temperature fluctuations can cause differences in the color development of the same batch of test strips, affecting the interpretation of test results. At the same time, the handling of samples and devices after testing lacks biosafety design, posing a risk of secondary transmission of Brucella. Utility Model Content

[0005] To overcome the shortcomings of existing technologies, the purpose of this utility model is to provide a nano-gold brucellosis detection device. By pulling outwards two clamping plates, the detection box is placed between the clamping plates, and a spring pushes the clamping plates to clamp the detection box. Then, the storage box is pushed into a protective box to avoid external factors interfering with the test results. The inside of the protective box is disinfected by turning on the ultraviolet disinfection lamp. The reflective coating enhances the reflection of ultraviolet rays, improves the disinfection effect, and avoids the impact of residual bacteria on the next test results.

[0006] The objective of this utility model is achieved through the following technical solution:

[0007] The nano-gold brucellosis detection device includes a protective box, a first tank, and a detection component. The first tank is opened through the left end of the protective box. A storage box is slidably installed on the inner wall of the first tank. Two clamping plates are slidably installed on the bottom of the inner wall of the storage box. Springs are fixed between the clamping plates and the inner wall of the storage box. A detection box is installed between the two clamping plates. Two symmetrically arranged ultraviolet disinfection lamps are fixed to the top of the inner wall of the protective box. The inner wall of the protective box is coated with a reflective coating. The detection component is installed inside the detection box.

[0008] In one optional embodiment, a second groove is provided at the bottom of the inner wall of the protective box. The second groove has an inverted T-shaped cross-section. A limit block is slidably provided on the inner wall of the second groove and is fixed to the lower end of the clamping plate.

[0009] In one optional embodiment, the upper end of the protective box is provided with an observation port, and the left end of the inner wall of the observation port is provided with a storage groove, and a light shield is slidably provided on the inner wall of the storage groove.

[0010] In one optional embodiment, a mounting box is fixed to the right end of the protective box, a fan is provided on the inner wall of the mounting box, and multiple ventilation slots are provided through the right end of the storage box, with the multiple ventilation slots being distributed at equal intervals.

[0011] In one optional embodiment, the detection assembly includes a base plate, the base plate is fixed to the bottom of the inner wall of the detection box, and a nitrocellulose membrane is disposed at the center of the upper part of the base plate.

[0012] In one optional embodiment, a colloidal gold pad is stacked on the left end of the nitrocellulose membrane, a sample pad is stacked on the left end of the colloidal gold pad, and an absorbent pad is stacked on the right end of the nitrocellulose membrane. The nitrocellulose membrane, colloidal gold pad, sample pad, and absorbent pad are arranged in a horizontal straight line.

[0013] In one optional embodiment, a cover plate is fixed to the upper end of the detection box, and a drip outlet is provided at the upper end of the cover plate, which is located above the sample pad. An observation groove is provided through the upper end of the cover plate.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0015] 1. Pull the two clamping plates outwards and place the test box between the clamping plates. The spring pushes the clamping plates to clamp the test box. Then push the storage box into the protective box to avoid external factors from interfering with the test results. Disinfect the inside of the protective box by turning on the ultraviolet disinfection lamp. The reflective coating enhances the reflection of ultraviolet rays, improves the disinfection effect, and avoids the residual bacteria from affecting the next test results.

[0016] 2. The observation port allows operators to observe test results without opening the protective box, reducing the risk of contamination. When the UV lamp is irradiating, closing the shield can prevent the UV lamp from shining through the observation port and causing harm to the staff. Ozone is generated during irradiation, and by turning on the fan, it is circulated inside, further improving the disinfection effect and reducing the spread of germs. Attached Figure Description

[0017] Figure 1 A three-dimensional structural diagram of a nano-gold brucellosis detection device;

[0018] Figure 2 A cross-sectional three-dimensional structural diagram of the storage box for the nano-gold brucellosis detection device;

[0019] Figure 3 A three-dimensional structural diagram of the clamping plate of the nano-gold brucellosis detection device;

[0020] Figure 4 A cross-sectional three-dimensional structural diagram of the light-shielding plate of the nano-gold brucellosis detection device;

[0021] Figure 5 This is a cross-sectional three-dimensional structural diagram of the detection box of the nano-gold brucellosis detection device.

[0022] In the diagram: 1. Protective box; 101. First tank; 102. Storage box; 103. Clamping plate; 104. Spring; 105. Detection box; 106. Ultraviolet disinfection lamp; 2. Second tank; 201. Base plate; 202. Nitrocellulose membrane; 203. Colloidal gold pad; 204. Sample pad; 205. Absorbent pad; 206. Cover plate; 207. Dropping port; 208. Observation tank; 3. Limiting block; 4. Observation port; 5. Storage tank; 6. Light shield; 7. Mounting box; 8. Fan; 9. Ventilation slot. Detailed Implementation

[0023] The present invention will now be further described in conjunction with the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments. Unless otherwise specified, the materials and equipment used in this embodiment are all commercially available. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this application, and should not be construed as limiting this application.

[0024] In the description of this application, it should be understood that the terms "upper," "lower," "front," "rear," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this application. In the description of this application, "a plurality of" means two or more, unless otherwise precisely specified.

[0025] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "connected," "linked," and "connected" should be interpreted broadly. For example, they can refer to a fixed connection, a connection through an intermediary, or a connection within two elements or an interaction between two elements. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0026] The terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such process, method, product, or apparatus.

[0027] Brucellosis is a zoonotic infectious disease caused by bacteria of the genus Brucella. The pathogen is highly contagious and pathogenic, and can be transmitted to humans through various routes, including contact with the secretions of infected animals, consumption of contaminated dairy products, or inhalation of bacterial aerosols. Globally, brucellosis is widespread in areas with developed livestock industries, and according to the World Health Organization (WHO), more than 500,000 new human cases are reported annually.

[0028] Brucellosis presents with complex and diverse clinical symptoms. Initially, it often manifests as nonspecific symptoms such as fever, fatigue, and joint pain, making it difficult to distinguish from influenza, rheumatism, and other diseases, leading to frequent misdiagnosis or missed diagnosis. If not diagnosed and treated promptly, the bacteria can invade vital organs such as the bone marrow, liver, and spleen, causing chronic infection, joint deformities, reproductive system damage, and even lifelong disability, placing a heavy burden on patients' families and society. In animal husbandry, cattle and sheep infected with brucellosis experience abortion, infertility, and decreased milk production, causing billions of yuan in economic losses to my country's livestock industry annually, while also increasing the risk of transmission to humans. Therefore, establishing rapid, accurate, and convenient brucellosis detection methods is crucial for early detection and control of outbreaks.

[0029] Currently, the commonly used methods for brucellosis detection in clinical and veterinary medicine mainly fall into three categories: etiological detection, serological detection, and molecular biological detection, but all of them have significant limitations.

[0030] Pathogen detection is the "gold standard" for diagnosing brucellosis, achieved by isolating and culturing Brucella from blood, bone marrow, or tissue samples from infected animals. This method boasts 100% specificity, but it is cumbersome and time-consuming. Brucella is a facultative intracellular parasite with slow growth; initial culture takes 5-7 days, or even 2-3 weeks, which is insufficient for rapid outbreak response. Furthermore, pathogen isolation requires a biosafety level 3 (BSL-3) laboratory, posing a high risk of infection. Primary healthcare institutions and veterinary stations often lack the necessary facilities, limiting its widespread application at the grassroots level.

[0031] Serological testing is currently the most widely used method for brucellosis screening, including the rose benzene plate agglutination test, tube agglutination test, and enzyme-linked immunosorbent assay (ELISA). Among these, the rose benzene plate agglutination test and tube agglutination test are routine methods for grassroots animal disease screening due to their simplicity and low cost. However, they suffer from insufficient sensitivity, and false negative results are common in the early stages of infection (within 1-2 weeks of onset) when antibody titers in the patient's serum are low. Furthermore, these methods cannot distinguish between natural infection and vaccine immunization, leading to numerous misdiagnoses in areas where brucellosis vaccination is widespread. While the ELISA method offers improved sensitivity and specificity, its operation relies on equipment such as ELISA readers, the testing process takes 2-3 hours, and the reagent stability is significantly affected by temperature, limiting its application in remote areas lacking cold chain facilities.

[0032] Molecular biology testing, represented by polymerase chain reaction (PCR), achieves rapid diagnosis by amplifying Brucella-specific gene fragments, reducing testing time to 3-4 hours. However, PCR technology has strict requirements for sample processing; components such as hemoglobin in blood and casein in milk samples can inhibit the PCR reaction, leading to false negatives. Furthermore, this method relies on sophisticated equipment and specialized technicians, resulting in high testing costs and hindering its widespread adoption in grassroots veterinary stations and rural areas. In addition, PCR products are prone to forming aerosols, causing cross-contamination and leading to false positive results, thus affecting the accuracy of the test.

[0033] With the development of nanobiotechnology, nanomaterials have shown great potential in the field of biodetection due to their unique optical, electrical, and biocompatibility. Gold nanoparticles, as a commonly used nanomaterial, possess characteristics such as uniform particle size, easy surface modification, and intuitive color changes. Their coupling reactions with biomolecules such as antibodies and nucleic acids are stable, and signals can be read through visual observation or simple optical devices, providing new ideas for constructing rapid and visual detection methods. Currently, immunochromatographic test strips based on gold nanoparticles have been applied in the detection of infectious diseases such as influenza and COVID-19, but research on their application in brucellosis detection is still in its early stages.

[0034] Existing nano-gold detection methods are mostly targeted at single antigens or antibodies, and the detection sensitivity can only reach the microgram level, which is difficult to meet the needs of early diagnosis of brucellosis. At the same time, the test strips have weak anti-interference ability and are easily affected by matrix effects in complex biological samples (such as whole blood and milk), resulting in unstable test results.

[0035] In practical applications, grassroots testing scenarios place extremely high demands on the portability, ease of operation, and readability of equipment. For example, veterinarians conducting on-site testing in pastoral areas need to complete sample testing within 15-30 minutes, with results that can be visually assessed; grassroots hospitals require low-cost, easy-to-operate equipment to rapidly screen suspected patients. However, existing testing equipment either relies on large instruments (such as ELISA readers and PCR machines), making them difficult to move, or lacks sufficient sensitivity and specificity to meet clinical diagnostic needs. Furthermore, most testing methods can only perform single-sample testing, making them unsuitable for large-scale disease screening and hindering the efficiency of brucellosis control efforts.

[0036] Please refer to Figures 1-5 This utility model provides an embodiment of a nano-gold brucellosis detection device, including a protective box 1; it also includes a first groove 101 and a detection component; the first groove 101 is opened through the left end of the protective box 1, a storage box 102 is slidably disposed on the inner wall of the first groove 101, two clamping plates 103 are slidably disposed on the bottom end of the inner wall of the storage box 102, a spring 104 is fixed between the clamping plates 103 and the inner wall of the storage box 102, a detection box 105 is disposed between the two clamping plates 103, two symmetrically arranged ultraviolet disinfection lamps 106 are fixedly disposed on the top end of the inner wall of the protective box 1, and a reflective coating is provided on the inner wall surface of the protective box 1; the inside of the detection box 105... The system is equipped with a detection component. The storage box 102 is pulled out from the first slot 101 of the protective box 1, and the two clamping plates 103 are pulled outward. The detection box 105 is placed between the clamping plates 103, and the spring 104 pushes the clamping plates 103 to clamp the detection box 105. Then, the storage box 102 is pushed into the protective box 1 to avoid interference from external factors with the detection results. The interior of the protective box 1 is disinfected by turning on the ultraviolet disinfection lamp 106 to prevent residual bacteria from affecting the next detection result. This solves the problem that in outdoor settings such as farms, where the temperature difference between day and night is large, temperature fluctuations can cause differences in the color development of the same batch of test strips, affecting the judgment of the detection results.

[0037] Please refer to Figures 1-4In a preferred embodiment of this utility model, a second groove 2 is provided at the bottom of the inner wall of the protective box 1. The second groove 2 has an inverted T-shaped cross-section. A limiting block 3 is slidably provided on the inner wall of the second groove 2. The limiting block 3 is fixed to the lower end of the clamping plate 103. An observation port 4 is provided at the upper end of the protective box 1. A storage groove 5 is provided at the left end of the inner wall of the observation port 4. A light shield 6 is slidably provided on the inner wall of the storage groove 5. An installation box 7 is fixed to the right end of the protective box 1. A fan 8 is provided on the inner wall of the installation box 7. Multiple ventilation slots 9 are provided through the right end of the storage box 102. The multiple ventilation slots 9 are equidistantly distributed. The observation port 4 allows operators to observe the test results without opening the protective box 1, reducing the risk of contamination. When the ultraviolet lamp is irradiated, the light shield 6 is closed to prevent the ultraviolet lamp from shining out through the observation port 4 and causing harm to the staff. Ozone is generated during the irradiation process. By turning on the fan 8, it is circulated inside, which further improves the disinfection effect and reduces the spread of germs.

[0038] Please refer to Figure 5 In a preferred embodiment of this invention, the detection assembly includes a base plate 201. The base plate 201 is fixedly connected to the bottom of the inner wall of the detection box 105. A nitrocellulose membrane 202 is disposed in the center of the upper part of the base plate 201. A colloidal gold pad 203 is stacked on the left end of the nitrocellulose membrane 202. A sample pad 204 is stacked on the left end of the colloidal gold pad 203. An absorbent pad 205 is stacked on the right end of the nitrocellulose membrane 202. The nitrocellulose membrane 202, colloidal gold pad 203, sample pad 204, and absorbent pad 205 are arranged in a horizontal straight line. A cover plate 206 is fixedly connected to the upper end of the detection box 105. A dropper 207 is disposed on the upper end of the cover plate 206, and the dropper 207 is located above the sample pad 204. An observation groove 208 is provided through the upper end of the cover plate 206. The detection lines and control lines on the nitrocellulose membrane 202 combine with the target-probe complex for color development. The absorption pad 205 absorbs excess sample and reagents, driving the chromatography process continuously. The horizontal linear distribution ensures a stable sample chromatography path and ensures the orderly progress of the detection reaction. The cover plate 206 seals the detection box 105 to prevent sample and reagent leakage and avoid external contamination of the detection components. The dropper 207 facilitates accurate dropping of the sample onto the sample pad 204, avoiding incorrect placement. The observation groove 208 corresponds to the detection lines and control lines of the nitrocellulose membrane 202, facilitating observation of the color development results without opening the detection box 105, making the operation hygienic and intuitive.

[0039] When in use, first pull the storage box 102 out of the first slot 101 of the protective box 1, pull the two clamping plates 103 outward, put the test box 105 between the clamping plates 103, and the spring 104 pushes the clamping plates 103 to clamp the test box 105. Then push the storage box 102 into the protective box 1 to prevent external factors from interfering with the test results.

[0040] The sample to be tested is added to the sample pad 204 through the dropper 207 of the cover plate 206 of the test box 105. The sample is subjected to horizontal chromatography under the siphon effect of the absorbent pad 205. It first flows through the sample pad 204 for pretreatment, and then enters the colloidal gold pad 203 to bind with the gold nanoparticle-labeled probe to form a target-probe complex. The complex continues to move to the nitrocellulose membrane 202 and binds to the specific antigen / antibody on the membrane. A color reaction occurs at the test line to determine whether brucellosis infection has occurred. Excess liquid is absorbed by the absorbent pad 205. After color development at the control line, the test result is confirmed to be valid.

[0041] After the test is completed, the observation port 4 is closed by sliding the light shield 6, and the inside of the protective box 1 is disinfected by turning on the ultraviolet disinfection lamp 106. The disinfection effect is enhanced by the reflective coating. Ozone is generated during the irradiation process. The fan 8 is turned on to circulate it inside, which further improves the disinfection effect and reduces the spread of germs.

[0042] Through the above steps, the storage box 102 is pulled out from the first slot 101 of the protective box 1, the two clamping plates 103 are pulled outward, the test box 105 is placed between the clamping plates 103, the spring 104 pushes the clamping plates 103 to clamp the test box 105, and then the storage box 102 is pushed into the protective box 1 to avoid external factors from interfering with the test results. The inside of the protective box 1 is disinfected by turning on the ultraviolet disinfection lamp 106 to avoid the residual bacteria affecting the next test results. This solves the problem that in outdoor scenarios such as farms, the temperature difference between day and night is large, and temperature fluctuations can cause differences in the color development of the same batch of test strips, affecting the judgment of the test results.

[0043] Although only certain components and embodiments of this application have been illustrated and described, many modifications and alterations (e.g., variations in the size, dimensions, structure, shape and proportion of the various elements, installation arrangement, material use, color, orientation, etc.) will be conceived by those skilled in the art without actually departing from the scope and spirit of the claims.

[0044] Finally, it should be noted that the above embodiments are only preferred embodiments of this utility model and should not be used to limit the scope of protection of this utility model. Any non-substantial changes and substitutions made by those skilled in the art based on this utility model shall fall within the scope of protection claimed by this utility model.

Claims

1. A nano-gold brucellosis detection device, characterized in that: It includes a protective box (1); it also includes a first tank (101) and a detection component; the protective box (1) has a first tank (101) through it on the left end, a storage box (102) is slidably arranged on the inner wall of the first tank (101), two clamping plates (103) are slidably arranged on the bottom of the inner wall of the storage box (102), a spring (104) is fixed between the clamping plate (103) and the inner wall of the storage box (102), a detection box (105) is arranged between the two clamping plates (103), two symmetrically arranged ultraviolet disinfection lamps (106) are fixedly connected to the top of the inner wall of the protective box (1), a reflective coating is provided on the inner wall surface of the protective box (1), and a detection component is provided inside the detection box (105).

2. The nano-gold brucellosis detection device according to claim 1, characterized in that: The bottom of the inner wall of the protective box (1) is provided with a second groove (2). The cross section of the second groove (2) is inverted T-shaped. A limit block (3) is slidably provided on the inner wall of the second groove (2). The limit block (3) is fixed to the lower end of the clamping plate (103).

3. The nano-gold brucellosis detection device according to claim 1, characterized in that: The protective box (1) has an observation port (4) at the top. The observation port (4) has a storage slot (5) on the left side of its inner wall. A light shield (6) is slidably installed on the inner wall of the storage slot (5).

4. The nano-gold brucellosis detection device according to claim 1, characterized in that: The protective box (1) is fixed to the right end of the mounting box (7), and the inner wall of the mounting box (7) is equipped with a fan (8). The right end of the storage box (102) is provided with multiple ventilation slots (9), which are distributed at equal intervals.

5. The nano-gold brucellosis detection device according to claim 1, characterized in that: The detection assembly includes a base plate (201), the bottom of the inner wall of the detection box (105) is fixed with the base plate (201), and a nitrocellulose membrane (202) is provided in the center of the upper end of the base plate (201).

6. The nano-gold brucellosis detection device according to claim 5, characterized in that: A colloidal gold pad (203) is stacked on the left end of the nitrocellulose membrane (202), a sample pad (204) is stacked on the left end of the colloidal gold pad (203), and an absorbent pad (205) is stacked on the right end of the nitrocellulose membrane (202). The nitrocellulose membrane (202), colloidal gold pad (203), sample pad (204), and absorbent pad (205) are arranged in a horizontal straight line.

7. The nano-gold brucellosis detection device according to claim 6, characterized in that: The upper end of the test box (105) is fixed with a cover plate (206), and a drip port (207) is provided on the upper end of the cover plate (206). The drip port (207) is located above the sample pad (204), and an observation groove (208) is provided through the upper end of the cover plate (206).

Images