Combined microscope spectroscopy system

Abstract

The utility model discloses a combined microscope spectrum system, including objective and sample stage still includes a plurality of with array arrangement's non -polarization beam splitter, and non -polarization beam splitter can rotate to change the emergent angle of light, and at least one non -polarization beam splitter can lead to sample platform with light path, and through rotating one or more non -polarization beam splitter can realize the light path intercommunication between any multiple non -polarization beam splitter, array external multiple light sources enter different outer edge non -polarization beam splitter, and allow access multiple and multiple signal receiver to accept objective or the light information of different outer edge non -polarization beam splitter, and this system can under the prerequisite that need not dismouting equipment flexibly adjust light path, switch light source or switch imaging mode.

Description

Technical Field

[0001] This utility model relates to microscopes, and more particularly to a combined microscope spectral system. Background Technology

[0002] Microscopic imaging spectroscopy is an indispensable observational tool in modern biomedicine, materials science, and other fields. Currently, systems that realize microscopic spectroscopy functions are mainly divided into two categories: one is an integrated microscopic system, which encapsulates the optical path in a fixed shell. It has the advantages of compact size and high stability. However, its optical path structure is fixed and closed, and users cannot freely modify or expand the system according to new experimental needs (such as switching different illumination methods or detection modes), resulting in extremely poor flexibility.

[0003] Another type is the coaxial construction scheme based on optical platforms or cage systems. This scheme constructs the optical path by aligning and installing individual optical components such as light sources, lenses, filters, and detectors one by one. Cage systems, in particular, use threaded rods to connect a series of standardized optical supports (such as cage plates or cage cubes) into a stable, rigid structure, offering advantages such as modularity, ease of assembly, and convenient optical path alignment. However, the functional flexibility of traditional cage systems remains limited: changing the optical path or replacing the light source usually requires manual disassembly and physical reassembly of the optical component cube. This process is not only cumbersome and time-consuming, but also requires recalibrating the optical path after reassembly, making dynamic real-time optical path switching difficult. Furthermore, it cannot support flexible combinations of multimodal parallel detection, such as performing spectral analysis or online system detection simultaneously with optical imaging. Summary of the Invention

[0004] Purpose of the utility model: The purpose of this utility model is to provide a combined microscope spectral system that can flexibly adjust the optical path, switch the light source, or switch the imaging mode without disassembling the equipment.

[0005] Technical Solution: The present invention provides a combined microscope spectral system, comprising an objective lens and a sample stage, and further comprising multiple non-polarizing beamsplitters arranged in an array. The non-polarizing beamsplitters can be rotated to change the light emission angle, and at least one non-polarizing beamsplitter can direct the light path to the sample stage. By rotating one or more non-polarizing beamsplitters, optical path communication between any two non-polarizing beamsplitters can be achieved. The array is externally provided with multiple light source interfaces to provide light sources to different outer edge non-polarizing beamsplitters, and multiple signal receivers to receive light information from the objective lens or different outer edge non-polarizing beamsplitters.

[0006] By constructing an array (optical matrix) consisting of multiple rotatable unpolarized beam splitters, the traditional microscope optical path is fundamentally changed from a fixed or manually reconfigurable model. By controlling the rotation angle of any beam splitter within the array, the optical path can be dynamically and arbitrarily reconfigured without physically moving components, guiding light from a specified source to the sample stage or signal light to a specified signal receiver. Furthermore, multiple light source interfaces and signal receivers are located outside the array, providing the system with multiple and different types of light sources or signal receivers, allowing for flexible selection based on needs. This improves the overall system flexibility and experimental efficiency, allowing for rapid switching between different imaging modes (such as bright field, dark field, or fluorescence).

[0007] Preferably, the row and column spacing of the non-polarizing beam splitter is adjustable.

[0008] This not only makes the entire system compatible with optical modules of different physical sizes, improving its versatility, but the spacing adjustment function also provides additional means of optical path control. Users can precisely control the optical path by adjusting the spacing or selecting different optical path paths to meet the application requirements of aberration correction or interferometry, which have special requirements for optical path.

[0009] Preferably, the number of rows and columns of the non-polarized beam splitter can be increased or decreased.

[0010] The system allows for the addition or removal of the number of rows and columns of the beam splitter, enabling modular expansion of the system. Users can flexibly configure the system scale according to actual needs and budget, scaling from a simple single-function system to a complex multimodal system.

[0011] Preferably, the plurality of light sources and signal receivers are arranged side by side or in parallel on different sides of the array formed by the non-polarizing beam splitter.

[0012] By optimizing the layout of the light source and signal receiver, and regularly placing them on different sides of the array, the system structure becomes more regular, reducing the complexity of the external optical path. Furthermore, placing them on different sides helps reduce stray light interference, improves signal quality, and facilitates system integration and routine maintenance.

[0013] Preferably, a non-polarizing beam splitter is provided between the objective lens and the signal receiver.

[0014] By placing a beam splitter between the objective lens and the signal receiver, true multimodal parallel detection is achieved. For example, the sample signal can be split into multiple beams at a certain ratio, allowing for simultaneous high-sensitivity imaging and real-time spectral analysis. This significantly improves data acquisition efficiency and research depth, providing a new solution for the analysis of complex samples.

[0015] Preferably, non-polarized beam splitters that can be withdrawn from the system and have a fixed tilt angle can be inserted between the plurality of rotatable non-polarized beam splitters.

[0016] The introduction of pluggable beamsplitters with fixed tilt angles provides users with greater configuration flexibility. Users can insert fixed beamsplitters with specific splitting ratios to achieve specific optical path functions based on experimental needs, without affecting the system's core dynamic reconfiguration capabilities. This hybrid configuration approach is both economical and practical, expanding the system's application range.

[0017] Beneficial effects: By adopting an array-based optical design based on rotatable non-polarizing beam splitters, and controlling the rotation angle of each beam splitter, users can dynamically and arbitrarily reconstruct the optical path without manually disassembling and assembling optical components, guiding the light from a specified light source to the sample stage, or guiding the signal light to a specified detector. While inheriting the modularity and high stability advantages of traditional cage systems, this system significantly improves the versatility of the equipment and experimental efficiency. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of the system. Figure 2 This is a three-dimensional schematic diagram of the overall structure of the system. Figure 1 ; Figure 3 This is a three-dimensional schematic diagram of the overall structure of the system. Figure 2 ; Figure 4 This is a schematic diagram of a cube structure equipped with a rotatable non-polarizing beam splitter. Detailed Implementation

[0019] like Figure 1 As shown, the combined microscope spectral system of this utility model includes an objective lens 1 and a sample stage 2, and also includes multiple non-polarizing beam splitters 3 arranged in an array. The non-polarizing beam splitters 3 can be rotated to change the light emission angle. At least one non-polarizing beam splitter 3 can guide the light path to the sample stage 2. By rotating one or more non-polarizing beam splitters 3, the light path between any two non-polarizing beam splitters 3 can be connected. The array is externally provided with multiple light source interfaces 4 to provide light sources to different outer edge non-polarizing beam splitters 3, and multiple signal receivers 5 to receive light information from the objective lens 1 or different outer edge non-polarizing beam splitters 3. Figure 1 Each component has been simplified. The light source interface 4 and signal receiver 5 actually contain multiple components, but they are arranged side by side, so they are simplified to be represented by a rectangle instead of each individual component (light source interface 4 or signal receiver 5) being represented separately.

[0020] The non-polarized beam splitters 3 are arranged in an array through the construction of a cage system. Specifically, multiple columns of longitudinal guide rods 7 are set on the optical platform 6. Multiple cubes 8 are slidably connected on every four longitudinal guide rods 7. Non-polarized beam splitters 3 are rotatably connected in the cubes 8. Cubes 8 at the same horizontal height are connected together by transverse guide rods 9. Non-polarized beam splitters 3 at the same horizontal height form the rows of the array. Non-polarized beam splitters 3 in the cubes 8 connected to the same four longitudinal guide rods 7 form the columns of the array.

[0021] A fixed threaded knob can be set on the cube 8 to fix it at a certain horizontal height. The longitudinal guide rod 7 can be fixed at different positions on the optical platform 6 to adjust the spacing of the cubes 8 in the horizontal direction. This allows adjustment of the row and column spacing of the unpolarized beam splitter 3. The number of longitudinal guide rods 7 can be increased or decreased as needed, and the number of cubes 8 connected to the same longitudinal guide rod 7 can also be increased or decreased as needed. Thus, the number of rows and columns of the unpolarized beam splitter 3 can also be increased or decreased as needed.

[0022] The cube 8 can be equipped with a coarse adjustment knob 10 and a fine adjustment knob 11 to adjust the rotation angle of the unpolarized beam splitter 3. Specifically, the coarse adjustment knob 10 is equipped with a clamping device for holding the unpolarized beam splitter 3, thus fixing it in place. Rotating the coarse adjustment knob 10 rotates the unpolarized beam splitter 3. Rotating the unpolarized beam splitter 3 adjusts the angle of the outgoing light, thereby reconstructing the optical path, and also adjusts the angle of the incident light on the sample stage, thus achieving different imaging effects. All six faces of the cube 8 can have through holes. The through holes on the front and back sides normally need to be sealed to reduce interference from external light. The four through holes on the top, bottom, left, and right sides are used for light transmission and can also be used to connect different instruments, such as objective lens 1, as needed. The top and bottom through holes of the cube 8 can also be sealed, respectively. The coarse adjustment knob 10 and the fine adjustment knob 11 are common components in optical instruments and their construction will not be described in detail here.

[0023] In addition to the rotatable unpolarized beam splitter 3, an unpolarized beam splitter 3 with a fixed tilt angle can be set at a location where optical path adjustment is not required (e.g., ...). Figure 2 and Figure 3 The two cubes 8 located in the middle row contain non-polarizing beam splitters 3 with a fixed tilt angle. Figure 2 and Figure 3 To highlight the main structure, objective lens 1, light source interface 4, and signal receiver 5 are omitted (these can be installed in their respective positions using conventional methods, so they are not shown). Moreover, these non-polarizing beam splitters 3 are installed in cube 8 in a pluggable manner, and can be removed from cube 8 and the entire system when not needed.

[0024] The non-polarized beam splitter 3 can be a non-polarized beam splitter plate or a non-polarized beam splitter cube. When it is not necessary to fold the optical path, the non-polarized beam splitter 3 can only be a non-polarized beam splitter plate. In this case, the non-polarized beam splitter plate should be set to be perpendicular to the optical path.

[0025] The multiple light source interfaces 4 and signal receivers 5 are arranged side-by-side or parallel on different sides of the array formed by the non-polarizing beam splitter 3. In this embodiment, the multiple light source interfaces 4 and signal receivers 5 are arranged in parallel, with all light source interfaces 4 located on the right side of the array and all signal receivers 5 located on the left side of the array.

[0026] With multiple light source interfaces 4, multiple light sources can be connected, and the types of light sources can be flexibly selected and set according to needs. This allows for flexible and dynamic switching of light sources as needed, without the need to disassemble or reassemble components in the system.

[0027] The signal receiver 5 can be a CCD or other optical signal receiving and processing device, such as a spectrometer. When it is a CCD, the light signal emitted from the objective lens can be used for optical imaging. Alternatively, the optical path can be adjusted so that the light passes directly to the CCD without passing through the sample and objective lens. In this case, recording the change of CCD pixel grayscale over time can detect the stability of the light source power, which is to perform online detection of the system.

[0028] In this embodiment, the objective lens 1 is positioned between the top leftmost row and the adjacent second row of unpolarized beam splitters 3. An independent signal receiver 5 (CCD) is located on the upper left side of the array to receive the light signal emitted from the objective lens. Alternatively, the objective lens 1 can be directed towards the signal receiver 5 on the left, eliminating the need for a separate signal receiver 5.

Claims

1. A combined microscope spectral system, comprising an objective lens (1) and a sample stage (2), characterized in that: It also includes multiple non-polarized beam splitters (3) arranged in an array. The non-polarized beam splitter (3) can be rotated to change the light emission angle. At least one non-polarized beam splitter (3) can lead the light path to the sample stage (2). By rotating one or more non-polarized beam splitters (3), the light path can be connected between any number of non-polarized beam splitters (3). Multiple light source interfaces (4) are provided outside the array to provide light sources to different outer edge non-polarized beam splitters (3), and multiple signal receivers (5) receive the light information from the objective lens (1) or different outer edge non-polarized beam splitters (3).

2. The combined microscope spectroscopic system according to claim 1, characterized in that: The row and column spacing of the non-polarized beam splitter (3) is adjustable.

3. The combined microscope spectroscopic system according to claim 1, characterized in that: The number of rows and columns of the non-polarized beam splitter (3) can be increased or decreased.

4. The combined microscope spectroscopic system according to claim 1, characterized in that: The non-polarized beam splitter (3) adopts a non-polarized beam splitter plate or a non-polarized beam splitter cube.

5. The combined microscope spectroscopic system according to claim 1, characterized in that: The multiple light source interfaces (4) and signal receivers (5) are respectively arranged side by side or side by side on different sides of the array formed by the non-polarizing beam splitter (3).

6. The combined microscope spectroscopic system according to claim 5, characterized in that: The objective lens (1) is equipped with a separate signal receiver (5) and the other signal receivers (5) are located on different sides of the array.

7. The combined microscope spectroscopic system according to claim 1, characterized in that: A non-polarizing beam splitter (3) is provided between the objective lens (1) and the signal receiver (5).

8. The combined microscope spectroscopic system according to claim 1, characterized in that: A non-polarized beam splitter (3) that can be extracted from the system and has a fixed tilt angle can be inserted between the plurality of rotatable non-polarized beam splitters (3).

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