A biosensor antibiotic analysis apparatus

Abstract

The utility model provides a kind of biological sensor antibiotic analysis equipment, belong to antibiotic analysis technical field.The rear of the antibiotic detection main part is provided with connecting line, and electrode clip assembly is set in one end of connecting line.Through the electrode clip assembly of setting in one end of connecting line on antibiotic detection main part, electrode clip assembly adopts rectangular plate, metal clamping plate and locking piece to be made of, can be clamped when clamping, electrode stick is clamped by metal clamping plate, and electrode stick is tightly clamped by two metal clamping plates using locking piece, avoid appearing in subsequent use process Shaking, even appear the situation of falling off, guarantee the stable detection of carrying out.

Description

Technical Field

[0001] This utility model relates to the field of antibiotic analysis technology, and more specifically, to a biosensor antibiotic analysis device. Background Technology

[0002] The pollution and emergence of drug-resistant bacteria caused by antibiotic overuse have become a serious global problem. The widespread use of antibiotics in livestock and poultry farming and disease treatment has led to a large amount of antibiotic residues entering the environment. Antibiotics that are not fully metabolized are discharged into soil and water bodies through hospital and domestic sewage, and have become a new pollutant indicator for food and environmental testing.

[0003] The current mainstream method for antibiotic detection is the liquid chromatography-electrochemical detector (LC-ECD) system. During detection, an electrode clamp is held on an electrode rod to form a pathway, enabling the detection of antibiotic content in the liquid. Currently, commonly used electrode clamps are controlled by torsion springs, which hold the electrode rod in place under the action of the torsion springs. However, if this clamping method comes into contact with electrical wires during use, it can cause the electrode clamp to wobble on the electrode rod, or even fall off in severe cases, resulting in unstable clamping and affecting the detection. Utility Model Content

[0004] To overcome the above deficiencies, this utility model provides a biosensor antibiotic analysis device that overcomes or at least partially solves the above technical problems.

[0005] This utility model is implemented as follows: This invention provides a biosensor antibiotic analysis device, including an antibiotic detection body, a connecting line at the rear of the antibiotic detection body, and an electrode clip assembly at one end of the connecting line.

[0006] In a preferred embodiment, the antibiotic detection body is placed on a base plate, and the base plate is provided with several perforated strips.

[0007] In a preferred embodiment, a biosensor is also placed on the base plate. The biosensor includes a base and a chip layer, with the chip layer disposed on the upper surface of the base, and the two are integrally formed.

[0008] In a preferred embodiment, the chip layer is further provided with microchannels, which are distributed in a serpentine pattern on the chip layer. The chip layer is also provided with liquid inlet holes and liquid collection holes.

[0009] In a preferred embodiment, an electrochemical reaction tank is further provided on the chip layer, and an electrode rod is disposed inside the electrochemical reaction tank.

[0010] In a preferred embodiment, the electrode clamp assembly includes a rectangular plate, a metal clamp, and a locking element.

[0011] In a preferred embodiment, the rectangular plate is made of metal, and the rectangular plate is connected to the connecting wire by crimping.

[0012] In a preferred embodiment, a metal clamp is fixedly installed on one side of the rectangular plate, and another metal clamp is slidably installed. The two metal clamps hold the electrode rod by locking components.

[0013] The biosensor antibiotic analysis equipment provided by this utility model has the following beneficial effects: An electrode clamp assembly is installed at one end of the connecting line on the antibiotic detection body. The electrode clamp assembly consists of a rectangular plate, a metal clamp, and a locking device. When clamping, the metal clamp holds the electrode rod, and the locking device firmly clamps the two metal clamping plates to the electrode rod, preventing shaking or even falling off during subsequent use and ensuring stable detection. Attached Figure Description

[0014] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a schematic diagram of the back structure of the antibiotic detection body of this utility model; Figure 3 This utility model Figure 2 Enlarged structural diagram at point A in the middle; Figure 4 This is a schematic diagram of the electrode clamp assembly of this utility model.

[0016] In the diagram: 1. Antibiotic detection body; 2. Connecting line; 3. Electrode clamp assembly; 31. Rectangular plate; 32. Metal clamp; 33. Locking element; 4. Base plate; 5. Biosensor; 51. Base; 52. Chip layer; 6. Microchannel; 7. Liquid inlet; 8. Liquid collection port; 9. Electrochemical reaction tank; 10. Electrode rod. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0018] Example Reference Figures 1-4 This utility model provides a technical solution: a biosensor antibiotic analysis equipment, including an antibiotic detection body 1, a connecting line 2 at the rear of the antibiotic detection body 1, and an electrode clip assembly 3 at one end of the connecting line 2. The antibiotic detection body 1 is a cuboid, and its shell is made of high-strength, corrosion-resistant engineering plastic material, which can effectively protect the internal precision components from the influence of the external environment and achieve high-precision detection of antibiotics. Its detection principle is based on the specific interaction between specific biorecognition elements and antibiotics, such as antigen-antibody reaction, nucleic acid hybridization, etc. By detecting and analyzing the signals generated by these interactions, the content of antibiotics in the sample can be accurately determined. The length of connecting line 2 is designed according to actual needs to meet different testing scenarios; The electrode clamp assembly 3 includes a rectangular plate 31, a metal clamp 32, and a locking member 33. The rectangular plate 31 is made of metal and is connected to the connecting wire 2 by crimping. A metal clamp 32 is fixedly installed on one side of the rectangular plate 31, and another metal clamp 32 is slidably installed. The two metal clamps 32 clamp the electrode rod 10 through the locking member 33. The locking member 33 includes a screw and a wing nut. The screw is located inside the two metal clamps 32, and the wing nut is located outside the metal clamps. When the wing nut is screwed onto the screw, it will push the movable metal clamp 32 to move. The movable metal clamp 32 works in conjunction with the fixed metal clamp 32 to clamp the electrode rod 10. By setting a groove on one side of the rectangular plate 31, setting a slider inside the groove, and fixing one of the metal clamps 32 to the slider, a movable metal clamp 32 is formed. The other metal clamp 32 is fixedly installed on one side of the rectangular plate 31. The two metal clamps 32 are in a relative state. In use, the movable metal clamp 32 can be moved by controlling the movement of the movable metal clamp 32 so that the two metal clamps 32 can be clamped on the electrode rod 10 for use.

[0019] In a preferred embodiment, the antibiotic detection body 1 is placed on a base plate 4, which has several perforated strips. The base plate 4 is made of a high-strength, corrosion-resistant, and well-insulated material, including engineering plastics such as polycarbonate (PC) and acrylonitrile-butadiene-styrene copolymer (ABS). These materials not only have high mechanical strength to withstand the weight of the antibiotic detection body 1, but also good chemical stability, making them less susceptible to corrosion from chemical reagents that may come into contact with during the detection process. Alternatively, the base plate 4 can also be made of metal, such as aluminum alloy, with a specially treated surface that ensures both strength and heat dissipation while also providing a certain degree of corrosion resistance.

[0020] In a preferred embodiment, a biosensor 5 is also placed on the base plate 4. The biosensor 5 includes a base 51 and a chip layer 52. The chip layer 52 is disposed on the upper surface of the base 51, and the two are integrally set. The base 51 serves as the supporting foundation for the biosensor 5 and is usually made of highly stable materials, such as polymethyl methacrylate (PMMA) or polydimethylsiloxane (PDMS). The thickness of the chip layer 52 is usually controlled between 50-200 μm to ensure that the structure is thin and suitable for lamination packaging, while maintaining the stability of the flow channels and electrodes. The layout of the microchannels 6 is optimized by computational fluid dynamics simulation to ensure that the flow rate deviation of antibiotic samples flowing into the detection areas of each electrode is <5%, ensuring the consistency of multi-channel detection. Furthermore, the design of the chip layer 52 and the base 51 being integrally set avoids antibiotic sample leakage caused by interlayer gaps, reduces sample loss and reduces the risk of cross-contamination. It is especially suitable for the detection of low concentration antibiotics in trace samples such as blood and environmental water samples. It also reduces the contact resistance between the electrode rod 10 and the base 51, ensuring that the attenuation during the transmission of detection signals is minimized and improving the accuracy of quantitative analysis of antibiotic concentration.

[0021] In a preferred embodiment, a microchannel 6 is provided on the chip layer 52. The microchannel 6 is distributed in a serpentine pattern on the chip layer 52. The chip layer 52 is also provided with an inlet hole 7 and a collection hole 8. The serpentine distribution can significantly extend the flow path of the fluid within the limited chip layer 52. When the antibiotic sample passes through the microchannel 6, it can fully contact the functionalized modification layer on the surface of the chip layer 52, such as the fixed nucleic acid nanoprobe and aptamer, prolonging the reaction time and thus improving the detection sensitivity. At the same time, the serpentine bend can generate moderate fluid disturbance, promote the mixing of sample and probe, and avoid the problem of insufficient reaction. The inlet end of the microchannel 6 is connected to the inlet hole 7, and the outlet end is connected to the collection hole 8, forming a complete fluid circulation path. The connection point adopts a smooth transition design to avoid fluid retention and pressure loss caused by right angles or sharp angles. In addition, the microchannel 6 is precisely positioned to correspond with the detection electrode array, ensuring that the antibiotic sample flowing through it can cover the electrode surface, so that the detection conditions of each channel electrode are consistent, further ensuring the reliability of multi-channel detection results. The microchannel 6, liquid inlet 7 and liquid collection hole 8 are compatible with the integrated structure of the base 51 and chip layer 52, ensuring the sealing and stability of the entire fluid system.

[0022] Meanwhile, the positions of the liquid inlet 7 and the liquid collection hole 8 do not hinder the connection between the biosensor 5 and the conductive contacts on the base 51, ensuring that the transmission of electrical signals is not affected, so that the biosensor 5 can work normally in conjunction with the antibiotic detection body 1 to achieve efficient and accurate detection of antibiotics.

[0023] An electrochemical reaction tank 9 is also provided on the chip layer 52. Electrode rods 10 are installed inside the electrochemical reaction tank 9. The inner wall of the electrochemical reaction tank 9 is specially treated to possess excellent chemical stability and insulation, preventing it from reacting with antibiotics, buffer solutions, etc., in the reaction system, and from interfering with the transmission of electrical signals. The electrochemical reaction tank 9 is connected to the microfluidic channel 6 via a narrow branch channel. The width and depth of the branch channel are optimized to precisely control the flow rate of fluid entering the electrochemical reaction tank 9, ensuring a stable concentration of the reaction system. The electrochemical reaction tank 9 is the core area where antibiotics undergo specific reactions and generate electrical signals. When an antibiotic sample is transported to this area via the microfluidic channel 6, it reacts with pre-added detection reagents such as enzyme markers and substrates in the electrochemical reaction tank 9. Simultaneously, under the influence of the electric field generated by the electrode rods 10, an electrochemical signal is formed.

[0024] Electrode rods 10 are vertically positioned inside the electrochemical reaction tank 9 and are typically made of noble metals such as gold, platinum, or carbon. These materials possess excellent conductivity, chemical stability, and electrochemical activity, ensuring a stable output electrical signal during the reaction. Electrode rods 10 have a small diameter, generally between 10 and 50 micrometers, with their tips polished into a smooth hemispherical shape to increase the contact area with the reaction liquid and reduce obstruction to fluid flow. Electrode rods 10 feature low background current and high sensitivity, enabling rapid response to minute electrical signal changes generated by antibiotic reactions. Their surface can be functionalized, such as by coating with a specific catalyst or antibody, further enhancing their specific recognition ability for antibiotic reactions and improving detection accuracy. Simultaneously, electrode rods 10 are highly corrosion-resistant and can be reused multiple times, reducing detection costs.

[0025] Specifically, the working process or working principle of a biosensor antibiotic analysis equipment is as follows: This equipment is based on the current mainstream antibiotic detection method, liquid chromatography-electrochemical detector (LC-ECD) system. During detection, the electrode clamp is held on the electrode rod 10 to form a pathway, thereby realizing the detection of antibiotic content in the liquid. Currently, commonly used electrode clamps are mostly controlled by torsion springs, which hold the electrode rod 10 under the action of the torsion springs. However, if the wires are touched during use, the electrode clamp on the electrode rod 10 will shake, and in severe cases, it may even fall off. The clamping is unstable, which affects the detection. This equipment is improved based on the problem. This device uses an electrode clamp assembly 3 to hold the electrode rod 10, and the locking member 33 can stably clamp the electrode clamp assembly 3 onto the electrode rod 10, preventing it from easily falling off. This allows the liquid to be tested to be dropped onto the biosensor 5, and the antibiotic in the liquid can be detected by the antibiotic detection body 1.

[0026] In specific operation, first clamp the electrode clamp assembly 3 onto the electrode rod 10, then connect it to the power supply, and connect the antibiotic detection body 1 to an external computer and open the detection software. After the preparation is completed, drip the treated liquid into the microchannel 6. The liquid will flow inside the microchannel 6 until it flows into the electrochemical reaction tank 9. When the liquid enters the electrochemical reaction tank 9, it will generate an electrochemical signal under the action of the electrode rod 10. The electrochemical signal is transmitted to the computer through the antibiotic detection body 1, and the detection data is displayed on the computer to complete the detection of antibiotics in the liquid.

[0027] When this device clamps the electrode rod 10 using the electrode clamp assembly 3, the wing nut on the screw is first loosened so that the metal clamp 32 can be fitted onto the electrode rod 10. After determining the clamping point, the wing nut is turned and turned deeper into the screw. During the turning process, the movable metal clamp 32 is pushed to move towards the fixed metal clamp 32 position until it is clamped onto the electrode rod 10. This allows the electrode clamp assembly 3 to be used stably and will not easily fall off.

[0028] This device employs photolithography to fabricate an 8-channel interdigitated gold electrode array. Computational fluid dynamics simulations using software such as COMSOL Multiphysics and ANSYS Fluent optimize the flow channel layout, ensuring consistent hydrodynamic characteristics across all detection units (flow rate deviation <5%). The electrode rod 10 surface is treated with oxygen plasma (50W, 30s) and then self-assembles into a monolayer using 1,6-hexanedithiol, significantly improving probe immobilization efficiency. Nucleic acid nanoprobes are functionalized and bound to aptamers, immobilizing the nanoprobes on the electrode rod 10 surface. An integrated sample pretreatment chamber, a built-in serpentine mixing channel, and a signal amplification reaction chamber are incorporated. The surface is modified with a DNAzyme-aptamer composite probe, forming a complete detection unit. Independent operation of multiple channels is achieved through lamination encapsulation.

Claims

1. A biosensor-based antibiotic analysis device, comprising an antibiotic detection body (1), characterized in that, A connecting line (2) is provided at the rear of the antibiotic detection body (1), and an electrode clamp assembly (3) is provided at one end of the connecting line (2). The electrode clamp assembly (3) includes a rectangular plate (31), a metal clamp (32) and a locking member (33). The rectangular plate (31) is made of metal, and the rectangular plate (31) is connected to the connecting line (2) by crimping.

2. The biosensor antibiotic analysis equipment according to claim 1, characterized in that, The antibiotic detection body (1) is placed on the base plate (4), and the base plate (4) has several hollow strips.

3. The biosensor antibiotic analysis equipment according to claim 2, characterized in that, A biosensor (5) will also be placed on the base plate (4). The biosensor (5) includes a base (51) and a chip layer (52). The chip layer (52) is disposed on the upper surface of the base (51), and the two are integrally disposed.

4. The biosensor antibiotic analysis equipment according to claim 3, characterized in that, The chip layer (52) is provided with microchannels (6), which are distributed in a serpentine pattern on the chip layer (52). The chip layer (52) is also provided with liquid inlet holes (7) and liquid collection holes (8).

5. The biosensor antibiotic analysis equipment according to claim 4, characterized in that, An electrochemical reaction tank (9) is also provided on the chip layer (52), and an electrode rod (10) is provided inside the electrochemical reaction tank (9).

6. The biosensor antibiotic analysis equipment according to claim 5, characterized in that, A metal clamp (32) is fixedly installed on one side of the rectangular plate (31), and another metal clamp (32) is slidably installed. The two metal clamps (32) clamp the electrode rod (10) through the locking member (33).

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