A plant tissue culture flask with adjustable ventilation

Abstract

The utility model relates to plant tissue culture technical field, and disclose a kind of plant tissue culture bottle of adjustable ventilation, including upper bottle body and lower bottle body, the lower bottle body rotation is connected in the bottom of upper bottle body, the outer wall of upper bottle body is fixedly connected with annular baffle, the outer wall of annular baffle and lower bottle body is all set with corresponding air hole, the inner wall of upper bottle body is provided with cleaning assembly, the cleaning assembly includes L board, the L board fixedly connected on the inner wall of upper bottle body, the sidewall of L board is set with through hole, and guiding rod is slidably connected in through hole. The plant tissue culture bottle of adjustable ventilation, by the rotation of annular baffle and drive scraper to rotate, utilize the elastic support force of spring to scraper, ensure that scraper is always closely combined with lower bottle body inner wall, complete bottle wall cleaning, continuously keep bottle wall transparent, convenient for operator real-time observation of the growth state of tissue culture seedling.

Description

Technical Field

[0001] This utility model relates to the field of plant tissue culture technology, specifically to a plant tissue culture bottle with adjustable aeration. Background Technology

[0002] In vitro culture of cells and tissues has become an indispensable part of life science research and practice. A wide variety of cell types are cultured, ranging from viruses to bacteria and fungi, and from human cells to animal and plant cells. Plant tissue culture is typically carried out in culture flasks. With the development of modern biotechnology, tissue culture technology has become increasingly widespread, and culture containers used in tissue culture have been extensively applied. However, existing culture flasks require the lid to be opened for aeration, and the aeration rate cannot be adjusted, preventing different plant tissues from being cultured in the same flask.

[0003] According to a plant tissue culture device for easy adjustment of aeration (publication number: CN213369215U), the above application uses a first bottle and a second bottle to adjust the aeration between the two bottles, which facilitates the culture of different types of plant tissues. This solves the problem that the aeration of previous culture bottles could not be adjusted, which prevented different plant tissues from being cultured in the same culture bottle.

[0004] However, during use, although the culture flasks are made of transparent material for easy observation, water vapor or culture medium residue can easily condense on the flask walls during long-term culture, affecting the clarity of observation. Utility Model Content

[0005] The purpose of this invention is to provide a plant tissue culture bottle with adjustable aeration to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a plant tissue culture bottle with adjustable ventilation, comprising an upper bottle body and a lower bottle body, wherein the lower bottle body is rotatably connected to the bottom of the upper bottle body, an annular baffle is fixedly connected to the outer wall of the upper bottle body, and corresponding ventilation holes are provided on the annular baffle and the outer wall of the lower bottle body, and a cleaning component is provided on the inner wall of the upper bottle body, the cleaning component comprising an L-plate, the L-plate being fixedly connected to the inner wall of the upper bottle body, a through hole being provided on the side wall of the L-plate, and a guide rod being slidably connected in the through hole, and a scraper being fixedly connected to the end of the guide rod, the scraper being in contact with the inner wall of the lower bottle body.

[0007] Preferably, an annular scale plate is fixedly connected to the outer wall of the lower bottle body, a connecting rod is fixedly connected to the bottom surface of the annular baffle, and a pointer is fixedly connected to the bottom end of the connecting rod.

[0008] Preferably, the outer wall of the annular baffle has a ring of arrayed toothed grooves.

[0009] Preferably, the annular baffle extends downward to the outer wall of the lower bottle body.

[0010] Preferably, the L-plate and the scraper are elastically connected by a spring, and the spring is sleeved on the outer wall of the guide rod.

[0011] Preferably, the pointer is in contact with the surface of the annular scale plate.

[0012] Compared with the prior art, this utility model provides a plant tissue culture bottle with adjustable aeration, which has the following beneficial effects:

[0013] 1. This plant tissue culture bottle with adjustable ventilation volume uses a rotating annular baffle to drive the scraper to rotate. The elastic support of the scraper by the spring ensures that the scraper is always in close contact with the inner wall of the lower bottle, thus cleaning the bottle wall and keeping the bottle wall transparent, allowing operators to observe the growth status of the tissue culture seedlings in real time.

[0014] 2. This plant tissue culture bottle with adjustable ventilation volume features a rotating annular baffle that drives a pointer to rotate synchronously. The annular scale plate, in conjunction with the pointer, visually displays the overlap ratio of the ventilation holes, allowing operators to precisely adjust the ventilation volume according to the needs of the plant's growth stage. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the disassembled upper and lower bottle body of this utility model;

[0017] Figure 3 This is a schematic diagram of the cleaning component structure of this utility model;

[0018] Figure 4 This is an enlarged structural diagram of point A in this utility model.

[0019] In the diagram: 1. Upper bottle body; 2. Lower bottle body; 3. Annular baffle; 31. Gear; 4. Vent hole; 5. Cleaning component; 51. L-plate; 52. Guide rod; 53. Scraper; 54. Spring; 6. Annular scale plate; 7. Connecting rod; 71. Pointer. Detailed Implementation

[0020] like Figures 1-4As shown, this utility model provides a technical solution: an adjustable aeration plant tissue culture bottle, including an upper bottle body 1 and a lower bottle body 2. The lower bottle body 2 is rotatably connected to the bottom of the upper bottle body 1, and a bottle cap is provided at the top of the upper bottle body 1. Plant tissue and culture medium are placed in the lower bottle body 2. An annular baffle 3 is fixedly connected to the outer wall of the upper bottle body 1. A ring of arrayed grooves 31 is formed on the outer wall of the annular baffle 3. The grooves 31 on the outer wall of the annular baffle 3 increase the friction during rotation, making it easier for the operator to apply force.

[0021] The annular baffle 3 extends downward to the outer wall of the lower bottle body 2. Corresponding ventilation holes 4 are provided on both the annular baffle 3 and the outer wall of the lower bottle body 2. By rotating the annular baffle 3, the overlapping area of ​​the annular baffle 3 and the ventilation holes 4 on the lower bottle body 2 is changed, thereby adjusting the ventilation volume. The ventilation volume is maximized when the ventilation holes 4 are completely overlapped, and minimized when they are completely offset.

[0022] A cleaning component 5 is provided on the inner wall of the upper bottle body 1. The cleaning component 5 includes an L-plate 51, which is fixedly connected to the inner wall of the upper bottle body 1. A through hole is opened on the side wall of the L-plate 51, and a guide rod 52 is slidably connected in the through hole. A scraper 53 is fixedly connected to the end of the guide rod 52. The scraper 53 is in contact with the inner wall of the lower bottle body 2. The L-plate 51 and the scraper 53 are elastically connected by a spring 54, which is sleeved on the outer wall of the guide rod 52. By rotating the annular baffle 3, the upper bottle body 1 rotates synchronously, causing the L-plate 51, the guide rod 52, and the scraper 53 to rotate synchronously. The spring 54 provides elastic support for the scraper 53, making the scraper 53 fit tightly against the inner wall of the lower bottle body 2. This cleans the condensed water vapor or adhering culture medium residue on the inner wall of the lower bottle body 2, keeping the bottle wall transparent for easy observation of the growth status of the tissue culture seedlings.

[0023] An annular scale plate 6 is fixedly connected to the outer wall of the lower bottle body 2. A connecting rod 7 is fixedly connected to the bottom surface of the annular baffle 3. A pointer 71 is fixedly connected to the bottom end of the connecting rod 7, and the pointer 71 is in contact with the surface of the annular scale plate 6. The rotation of the annular baffle 3 drives the pointer 71 to rotate synchronously. Through the cooperation of the annular scale plate 6 and the pointer 71, the overlap ratio of the ventilation holes 4 can be displayed intuitively, which makes it easy for operators to accurately adjust the ventilation volume according to the needs of the plant growth stage.

[0024] In this invention, during use, the operator holds the annular baffle 3, and the array of teeth 31 on its outer wall effectively increases the friction between the hand and the annular baffle 3, facilitating stable force application. When the annular baffle 3 is rotated, the upper bottle body 1 rotates synchronously with the annular baffle 3, and the pointer 71 rotates with the annular baffle 3. The pointer 71 points to the scale on the annular scale plate 6, which can intuitively reflect the overlap ratio between the annular baffle 3 and the vent hole 4 on the lower bottle body 2. If it is necessary to increase the ventilation volume, the annular baffle 3 can be rotated to make the pointer 71 point to the corresponding high overlap scale until the vent hole 4 is completely overlapped; if it is necessary to decrease the ventilation volume, the annular baffle 3 can be rotated in the opposite direction to make the pointer 71 point to the low overlap scale until the vent hole 4 is completely misaligned, thereby achieving precise control of the ventilation volume. During the cultivation process, if condensation forms on the inner wall of the lower bottle 2 due to temperature changes, or if residual culture medium adheres, the annular baffle 3 can be rotated again. As the upper bottle 1 rotates, the L-plate 51 fixed to its inner wall simultaneously rotates the guide rod 52 and the scraper 53. Since the L-plate 51 and the scraper 53 are elastically connected by a spring 54 sleeved on the outer wall of the guide rod 52, the spring 54 always applies a spring force towards the bottle wall to the scraper 53, ensuring a tight fit between the scraper 53 and the inner wall of the lower bottle 2. With the rotation of the scraper 53, the condensed condensation and adhering culture medium residue on the inner wall are scraped away, keeping the lower bottle 2 transparent. This allows operators to easily observe the growth status of the tissue culture seedlings inside, ensuring the smooth progress of the cultivation process.

[0025] The present invention has been described in detail above. However, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, any modifications or improvements that do not depart from the spirit of the present invention are within the protection scope of the present invention.

Claims

1. A plant tissue culture flask with adjustable aeration, comprising an upper body (1) and a lower body (2), characterized in that: The lower bottle body (2) is rotatably connected to the bottom of the upper bottle body (1). An annular baffle (3) is fixedly connected to the outer wall of the upper bottle body (1). Corresponding ventilation holes (4) are provided on the annular baffle (3) and the outer wall of the lower bottle body (2). A cleaning component (5) is provided on the inner wall of the upper bottle body (1). The cleaning component (5) includes an L plate (51). The L plate (51) is fixedly connected to the inner wall of the upper bottle body (1). A through hole is provided on the side wall of the L plate (51), and a guide rod (52) is slidably connected in the through hole. A scraper (53) is fixedly connected to the end of the guide rod (52). The scraper (53) is in contact with the inner wall of the lower bottle body (2).

2. The plant tissue culture flask with adjustable aeration according to claim 1, characterized in that: The outer wall of the lower bottle body (2) is fixedly connected to an annular scale plate (6), the bottom surface of the annular baffle (3) is fixedly connected to a connecting rod (7), and the bottom end of the connecting rod (7) is fixedly connected to a pointer (71).

3. The plant tissue culture flask with adjustable aeration according to claim 1, characterized in that: The outer wall of the annular baffle (3) has a ring of arrayed toothed grooves (31).

4. A plant tissue culture flask with adjustable aeration according to claim 1, characterized in that: The annular baffle (3) extends downward to the outer wall of the lower bottle body (2).

5. A plant tissue culture flask with adjustable aeration according to claim 1, characterized in that: The L-plate (51) and the scraper (53) are elastically connected by a spring (54), which is sleeved on the outer wall of the guide rod (52).

6. A plant tissue culture flask with adjustable aeration according to claim 2, characterized in that: The pointer (71) is in contact with the surface of the annular scale plate (6).

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