A rapid sectioning device for animal frozen tissue

Abstract

This utility model discloses a rapid slicing device for frozen animal tissues in the field of biological experimental technology, comprising: a blade holder with a detachable and fixed slicing blade at one end; a sample head located on the side of the slicing blade away from the blade holder, used to hold the frozen tissue sample to be sliced, and capable of adjusting the spatial position of the frozen tissue sample to cooperate with the slicing blade for layer-by-layer cutting; and a conveying component embedded in the blade holder, the conveying path of the conveying component extending away from the slicing blade, the conveying component being used to receive frozen slices sliding off the slicing blade and drive the frozen slices to move intermittently along the conveying path. The intermittent movement of the conveying component of this device realizes automatic separation and orderly temporary storage of slices, transforming the traditional slicing and picking operation into a slicing and temporary storage operation, realizing a new mode of continuous slicing followed by centralized picking, greatly reducing the number of times the anti-roll plate is repeatedly opened and closed, and significantly improving the efficiency of batch slicing.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental technology, specifically to a rapid slicing device for frozen animal tissues. Background Technology

[0002] Frozen sectioning for histopathology is a key technique in clinical pathological diagnosis, widely used in rapid intraoperative pathological assessment, differentiation of benign and malignant tumors, and determination of lesion boundaries. Existing frozen section machines typically consist of core components such as a blade holder, sample head, and anti-roll plate. The workflow is as follows: fresh tissue is fixed with embedding agent and rapidly frozen, then mounted on the sample head; the sample head's lifting and feeding motion brings the tissue into contact with the cutting blades on the blade holder for layer-by-layer cutting; the slices slide down the anti-roll plate to the receiving area of ​​the blade holder, and the operator must open the anti-roll plate after each slice, attaching the slice to a glass slide before proceeding to the next cut.

[0003] It employs a slicing and retrieval operation mode, requiring a complete cycle of closing the anti-roll plate, cutting, opening the anti-roll plate, and retrieval for each slice. The operation steps are cumbersome and repetitive. For scenarios requiring batch slicing, such as multi-lesion samples or animal experiments, repeatedly opening and closing the anti-roll plate not only prolongs the operation time but also causes temperature fluctuations in the freezing chamber, affecting the quality of subsequent slices.

[0004] To address these issues, a rapid slicing device for frozen animal tissues is provided. Utility Model Content

[0005] The purpose of this invention is to provide a rapid slicing device for frozen animal tissues, which solves the problem that existing slicing and retrieval operations require frequent opening and closing of the anti-roll plate, making it difficult to efficiently obtain multiple slices.

[0006] This utility model achieves the above objectives through the following technical solutions: A rapid slicing device for frozen animal tissues, comprising: The blade holder has a detachable and fixed slicing blade at one end; The sample head is located on the side of the slicing blade away from the blade holder. It is used to hold the frozen tissue sample to be cut and can adjust the spatial position of the frozen tissue sample to cooperate with the slicing blade to achieve layer-by-layer cutting. A conveying assembly is embedded in the blade holder. The conveying path of the conveying assembly extends away from the slicing blade. The conveying assembly is used to receive frozen slices that slide off the slicing blade and drive the frozen slices to move intermittently along the conveying path to achieve automatic separation and orderly temporary storage of frozen slices. An anti-roll plate is installed on top of the tool holder.

[0007] As a further optimization of this utility model, the conveying assembly includes two parallel and oppositely arranged mounting plates, a plurality of transmission rollers rotatably disposed between the two mounting plates, a conveyor belt body sleeved on the outer periphery of the plurality of transmission rollers, and a drive motor for driving at least one of the transmission rollers to rotate; the top surface of the conveyor belt body is flush with the top surface of the knife holder.

[0008] As a further optimization of this utility model, the anti-roll plate includes a frame and a transparent plate with a hollowed-out area in the middle of the frame; one side of the frame is provided with a hinge seat that is rotatably connected to the blade holder, and the other side is provided with a support leg that overlaps the blade holder; the end of the frame is provided with a handle.

[0009] As a further optimization of this utility model, a synchronization component is also included. The synchronization component is used to match the driving timing of the delivery component with the motion cycle of the sample head. The synchronization component includes a bracket fixed on the tool holder, two sensors fixed on the bracket, a sensor fixed on the side of the sample head, and a controller fixed on the tool holder. The two sensors are arranged vertically at intervals along the lifting path of the sample head. The sensors are electrically connected to the input terminal of the controller, and the output terminal of the controller is electrically connected to the drive motor.

[0010] As a further optimization of this utility model, it also includes a heat insulation component located at the bottom of the anti-roll plate. The heat insulation component is used to release a low-temperature medium around the conveying component to form a low-temperature zone and maintain the stability of the frozen slice morphology.

[0011] As a further optimization of this utility model, the heat preservation component includes a distribution manifold fixedly disposed at the bottom of the frame and a conveying main pipe connected to the distribution manifold; the distribution manifold has a U-shaped structure and is provided with a plurality of nozzles evenly distributed thereon, and the end of the conveying main pipe away from the distribution manifold is used to connect to a low-temperature medium supply device.

[0012] The beneficial effects of this utility model are as follows: 1. The intermittent movement of the conveying component of this utility model realizes the automatic separation and orderly temporary storage of frozen slices, transforming the traditional slicing and picking operation into a slicing and temporary storage operation, realizing a new mode of continuous slicing followed by centralized picking, greatly reducing the number of times the anti-roll plate is repeatedly opened and closed, and significantly improving the efficiency of batch slicing.

[0013] 2. This utility model creates a local low-temperature zone around the conveyor belt to quickly freeze the slices and maintain their morphological integrity, effectively suppressing thermal deformation caused by temperature fluctuations. At the same time, the low-temperature environment allows the slices to maintain suitable physical hardness, ensuring that they maintain a high-quality, distortion-free morphology throughout the entire temporary storage and pick-up process, thereby obtaining high-quality slices that are continuous, flat, and have good stainability. Attached Figure Description

[0014] Figure 1 This is a three-dimensional schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the conveying component structure of this utility model; Figure 3 This is a schematic diagram of the synchronization component structure of this utility model; Figure 4 This is a schematic diagram of the thermal insulation component structure of this utility model.

[0015] In the picture: 1. Blade holder; 101. Slicing blade; 2. Sample head; 3. Conveying assembly; 301. Mounting plate; 302. Drive roller; 303. Conveyor belt; 304. Drive motor; 4. Anti-roll plate; 401. Frame; 402. Transparent plate; 403. Hinge seat; 404. Support leg; 405. Handle; 5. Synchronization assembly; 501. Sensor; 502. Bracket; 503. Sensing element; 504. Controller; 6. Insulation assembly; 601. Distribution manifold; 602. Nozzle; 603. Main conveying pipe. Detailed Implementation

[0016] The present application will now be described in further detail with reference to the accompanying drawings. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0017] Example 1 To address the issue that traditional slicing and slide removal operations require frequent opening and closing of the anti-roll plate 4, making it difficult to efficiently obtain multiple frozen sections, please refer to [link to relevant documentation]. Figures 1-3 This utility model provides a rapid slicing device for frozen animal tissues, comprising: The blade holder 1 has a detachable and fixed slicing blade 101 at one end, and the blade holder 1 has a mounting groove along the length of the slicing blade 101. The sample head 2 is located on the side of the slicing blade 101 away from the blade holder 1. It is used to hold the frozen tissue sample to be cut and can adjust the spatial position of the frozen tissue sample to cooperate with the slicing blade 101 to achieve layer-by-layer cutting. The spatial position adjustment of the sample head 2 adopts the existing technology, which usually includes a lifting drive mechanism and a feeding drive mechanism to achieve precise displacement control of the frozen tissue sample to be cut in the vertical and horizontal directions, thereby completing the layer-by-layer cutting action. The conveying component 3 is embedded in the blade holder 1. The conveying path of the conveying component 3 extends away from the slicing blade 101. The conveying component 3 is used to receive frozen slices that slide off the slicing blade 101 and drive the frozen slices to move intermittently along the conveying path to achieve automatic separation and orderly temporary storage of frozen slices. An anti-roll plate 4 is placed on top of the blade holder 1. One end of the anti-roll plate 4 extends above the blade of the slicing blade 101 to guide the frozen slices to slide onto the conveying assembly 3 and prevent them from curling.

[0018] The conveying assembly 3 includes two parallel and oppositely arranged mounting plates 301, a plurality of drive rollers 302 rotatably disposed between the two mounting plates 301, a conveyor belt body 303 sleeved on the outer periphery of the plurality of drive rollers 302, and a drive motor 304 for driving at least one of the drive rollers 302 to rotate; the top surface of the conveyor belt body 303 is flush with the top surface of the cutter holder 1 to ensure that the frozen slices can slide in smoothly.

[0019] The anti-roll plate 4 includes a frame 401 and a transparent plate 402 located in the hollowed-out area in the middle of the frame 401; one side of the frame 401 is provided with a hinge seat 403 that is rotatably connected to the knife holder 1, so that the anti-roll plate 4 can be flipped open and closed around the hinge seat 403; the other side is provided with a support leg 404 that overlaps the knife holder 1; and the end of the frame 401 is provided with a handle 405, which makes it convenient for operators to open or close the anti-roll plate 4.

[0020] In use, the slicing blade 101 is detachably fixed to the end of the blade holder 1, ensuring that the blade faces the sample head 2. The frozen tissue sample to be sliced ​​is fixed on the sample head 2, and the anti-roll plate 4 is placed on top of the blade holder 1. After starting the device, the sample head 2 moves the frozen tissue sample to be sliced. After a single layer of slicing is completed, the slice slides down to the receiving area of ​​the conveyor belt 303 under the guidance of the anti-roll plate 4. During the upward return of the sample head 2, the drive motor 304 starts and drives the transmission roller 302 to rotate, so that the conveyor belt 303 moves a preset distance along the conveying path to match the slice width, and the received slice is transferred to the temporary storage area. At the same time, a new receiving position is reserved for the next slice. The above cutting and conveying cycle is repeated until a preset number of frozen slices are made. After all frozen slices are completed, the anti-roll plate 4 is flipped open by the handle 405 to expose the slices arranged in an orderly manner on the conveyor belt 303. The operator uses a glass slide to attach the slices one by one for subsequent fixation, staining and other treatments.

[0021] like Figure 1 , Figure 3As shown, it also includes a synchronization component 5, which is used to match the driving timing of the conveying component 3 with the motion cycle of the sample head 2 to ensure that the slice conveying action is performed only in the non-cutting stage; the synchronization component 5 includes a bracket 502 fixed on the tool holder 1, two sensors 501 fixed on the bracket 502, a sensor 503 fixed on the side of the sample head 2, and a controller 504 fixed on the tool holder 1; the two sensors 501 are arranged vertically at intervals along the lifting path of the sample head 2, the sensors 501 are electrically connected to the input terminal of the controller 504, and the output terminal of the controller 504 is electrically connected to the drive motor 304.

[0022] When the sample head 2 moves downward to perform the slicing operation, the sensor 503 on its side surface moves synchronously with it. The sensor 503 passes through two sensors 501 in sequence. The controller 504 collects the input signals of the two sensors 501 in real time and determines the direction of movement of the sample head 2 by comparing their triggering order. If the lower sensor 501 is detected to trigger first and the upper sensor 501 is triggered later, it is determined to be an upward stroke; otherwise, it is a downward stroke. Only when it is determined to be an upward stroke, the controller 504 outputs a start pulse signal to the drive motor 304 to drive the conveyor belt 303 forward by a preset step distance, moving the cut tissue slice to the next station. During the downward stroke, the controller 504 keeps the conveyor assembly 3 stationary to avoid interference with the slicing process due to the movement of the conveyor belt 303, thus ensuring stable slice quality.

[0023] The interaction between sensor 501 and sensing element 503 can be flexibly configured according to the actual application environment and installation conditions, offering a variety of optional technical implementation schemes. In one embodiment, sensor 501 is a Hall sensor, and sensing element 503 is a permanent magnet. When sample head 2 moves up and down, the permanent magnet moves synchronously with it and passes through the sensing area of ​​the Hall sensor, causing a change in magnetic field strength to detect the position signal. In another embodiment, sensor 501 is a through-beam photoelectric switch, including a transmitter and a receiver positioned opposite each other. They are used in pairs and installed face-to-face. Sensing element 503 is a light-shielding plate. When the light-shielding plate passes through the transmitter and receiver, it blocks the light signal, thereby detecting the position signal.

[0024] Example 2 Based on Example 1, to address the issue that frozen slices on the conveying assembly 3, when exposed to room temperature, are prone to softening due to heat absorption and loss of structural stability, leading to the risk of slice breakage and quality degradation, such as... Figure 1 , Figure 4 As shown, it also includes a heat insulation component 6 located at the bottom of the anti-roll plate 4. The heat insulation component 6 is used to release a low-temperature medium around the conveying component 3 to form a low-temperature zone and maintain the stability of the frozen slice shape. The low-temperature medium includes, but is not limited to, liquid nitrogen, cold air, etc.

[0025] The insulation component 6 includes a distribution manifold 601 fixedly disposed at the bottom of the frame 401, and a delivery main pipe 603 connected to the distribution manifold 601. The distribution manifold 601 has a U-shaped structure to achieve symmetrical cooling of both sides of the slice. It is provided with a plurality of equally spaced nozzles 602. The end of the delivery main pipe 603 away from the distribution manifold 601 is used to connect to a low-temperature medium supply device.

[0026] The main conveyor pipe 603 is connected to an external cryogenic medium supply device (such as a liquid nitrogen storage tank or a cryogenic air compressor). The medium pressure and flow rate are regulated by the supply device, and the cryogenic medium is delivered to the distribution manifold 601 through the main conveyor pipe 603. The medium is evenly released to both sides of the frozen slices on the conveyor belt 303 through multiple equally spaced nozzles 602, forming a stable local cryogenic zone around the frozen slices. During the slice cutting and conveying process, the insulation component 6 continuously releases the cryogenic medium to compensate for the temperature rise caused by blade friction heat and ambient radiation heat. When the anti-roll plate 4 is opened to remove the slice, the medium supply stops. This solves the problem that the frozen slices on the conveyor component 3 may locally heat up due to blade friction heat, room temperature radiation, and long operation time, leading to deformation, breakage, and quality degradation of the frozen slices.

[0027] The embodiments described above are merely examples of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these modifications and improvements all fall within the protection scope of this utility model.

Claims

1. A rapid slicing device for frozen animal tissues, characterized in that, include: The blade holder (1) has a detachable and fixed slicing blade (101) at one end. The sample head (2) is located on the side of the slicing blade (101) away from the blade holder (1), and is used to hold the frozen tissue sample to be cut, and can adjust the spatial position of the frozen tissue sample to cooperate with the slicing blade (101) to achieve layer-by-layer cutting. The conveying component (3) is embedded in the blade holder (1). The conveying path of the conveying component (3) extends away from the slicing blade (101). The conveying component (3) is used to receive frozen slices that slide off the slicing blade (101) and drive the frozen slices to move intermittently along the conveying path to achieve automatic separation and orderly temporary storage of frozen slices. An anti-roll plate (4) is placed on top of the knife holder (1).

2. The rapid slicing device for frozen animal tissue according to claim 1, characterized in that, The conveying assembly (3) includes two parallel and oppositely arranged mounting plates (301), a plurality of drive rollers (302) rotatably disposed between the two mounting plates (301), a conveyor belt (303) sleeved on the outer periphery of the plurality of drive rollers (302), and a drive motor (304) for driving at least one of the drive rollers (302) to rotate. The top surface of the conveyor belt (303) is flush with the top surface of the tool holder (1).

3. The rapid slicing device for frozen animal tissue according to claim 1, characterized in that, The anti-roll plate (4) includes a frame (401) and a transparent plate (402) located in the hollow area in the middle of the frame (401). The frame (401) has a hinge seat (403) on one side that is rotatably connected to the knife holder (1), and a support leg (404) on the other side that overlaps the knife holder (1). The end of the frame (401) is provided with a handle (405).

4. The rapid slicing device for frozen animal tissue according to claim 2, characterized in that, It also includes a synchronization component (5) for matching the driving timing of the delivery component (3) with the motion cycle of the sample head (2); The synchronization component (5) includes a bracket (502) fixed on the tool holder (1), two sensors (501) fixed on the bracket (502), a sensor (503) fixed on the side of the sample head (2), and a controller (504) fixed on the tool holder (1). The two sensors (501) are arranged vertically at intervals along the lifting path of the sample head (2). The sensors (501) are electrically connected to the input terminal of the controller (504), and the output terminal of the controller (504) is electrically connected to the drive motor (304).

5. A rapid slicing device for frozen animal tissue according to claim 3, characterized in that, It also includes a heat insulation component (6) located at the bottom of the anti-roll plate (4), which is used to release a low temperature medium around the conveying component (3) to form a low temperature zone and maintain the stability of the frozen slice morphology.

6. A rapid slicing device for frozen animal tissues according to claim 5, characterized in that, The thermal insulation component (6) includes a distribution manifold (601) fixedly disposed at the bottom of the frame (401) and a main conveying pipe (603) connected to the distribution manifold (601). The distribution manifold (601) has a U-shaped structure and is provided with a plurality of equally spaced nozzles (602). The end of the main delivery pipe (603) away from the distribution manifold (601) is used to connect to a cryogenic medium supply device.

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