A low-temperature continuous reaction system for plant small molecule peptides based on composite enzymolysis

Abstract

The utility model relates to biological extraction equipment technical field discloses a kind of plant small molecule peptide low-temperature continuous reaction system based on composite enzymolysis, including two groups of reaction mechanism, the outside of one group of reaction mechanism is provided with conveying mechanism, the conveying mechanism includes storage tank, the outside of the storage tank is provided with crushing mechanism, the first crushing of the device is carried out by two groups of reverse rotation roller, secondary crushing is carried out again by multiple groups of grinding blade, forms slurry raw material, double crushing structure can significantly improve raw material degree of crushing, provide more sufficient reaction basis for subsequent enzymolysis, by first gear drive meshing three groups of second gear, make first blade and multiple groups of second blade collaborative rotation, multidimensional stirring design can ensure that material is fully mixed, eliminate stirring dead angle, avoid excessive stirring simultaneously to produce too much foam, guarantee reaction environment stable, by temperature sensor and PID controller linkage.

Description

Technical Field

[0001] This utility model relates to the field of bio-extraction equipment technology, specifically to a low-temperature continuous reaction system for plant small molecule peptides based on compound enzymatic hydrolysis. Background Technology

[0002] Complex enzymatic hydrolysis is a highly efficient and targeted biocatalytic technology that overcomes the limitations of single enzymatic hydrolysis through the synergistic effect of multiple enzymes. It can more thoroughly, efficiently, and controllably decompose biological macromolecules into valuable small molecule products and has a wide range of applications from food to energy.

[0003] Existing plant protein peptide extraction methods often employ high-temperature acid-base methods, which easily damage the active structure of peptides and result in a wide molecular weight distribution and low target yield. While enzymatic hydrolysis is gentler, it suffers from low hydrolysis efficiency, incomplete reaction, large batch-to-batch variability, and long reaction times. Ordinary reaction vessels lack sufficient temperature control precision, affecting enzyme activity and extraction efficiency. Therefore, those skilled in the art provide a low-temperature continuous reaction system for plant small molecule peptides based on compound enzymatic hydrolysis to address the problems mentioned in the background. Summary of the Invention

[0004] The purpose of this invention is to provide a low-temperature continuous reaction system for plant small molecule peptides based on compound enzymatic hydrolysis, thereby solving the problems mentioned in the background section of the prior art.

[0005] This utility model provides the following technical solution: a low-temperature continuous reaction system for plant small molecule peptides based on compound enzymatic hydrolysis, including a roll body, including two sets of reaction mechanisms, a conveying mechanism is provided on the outside of one set of reaction mechanisms, the conveying mechanism includes a storage box, and a crushing mechanism is provided on the outside of the storage box.

[0006] As a preferred embodiment of the above technical solution, the reaction mechanism includes a reaction vessel. A pH probe is fixedly connected to the upper part of the inner side of the reaction vessel. An acid metering pump is installed at the upper end of the reaction vessel on one side of the pH probe, and an alkali metering pump is installed at the upper end of the reaction vessel on the other side of the pH probe. A jacket is formed inside the reaction vessel. A circulating coolant is fixedly connected to the inner side of the jacket. A temperature sensor is fixedly connected to the front end of the reaction vessel outside the circulating coolant. A PID controller is installed at the front end of the reaction vessel on one side of the temperature sensor. A connecting pipe is provided between the two sets of reaction vessels through the jacket. The two ends of the connecting pipe are fixedly connected to the two sets of reaction vessels respectively.

[0007] As a preferred embodiment of the above technical solution, a first gear is rotatably connected to the inner side of the reaction vessel near the lower end. A first drive shaft is fixedly connected to the inner side of the first gear. A partition is rotatably connected to the outer side of the first drive shaft. Multiple sets of first blades are fixedly connected to the outer side of the first drive shaft. Three sets of second gears are meshed with the outer side of the first gear. One end of the second gear is rotatably connected to the reaction vessel. The other end of the second gear is fixedly connected to a second drive shaft. The outer side of the second drive shaft is rotatably connected to the partition. Multiple sets of second blades are fixedly connected to the outer side of the second drive shaft.

[0008] As a preferred embodiment of the above technical solution, a first synchronous pulley is fixedly connected to the outer side of the reaction vessel at the lower end of the first gear, and a first synchronous belt is rotatably connected to the outer sides of the two sets of first synchronous pulleys. A first motor is installed at the lower end of one set of the reaction vessels, and the output shaft of the first motor is fixedly connected to a set of first synchronous pulleys.

[0009] As a preferred embodiment of the above technical solution, the conveying mechanism includes a conveying pipe, one end of which is fixedly connected to a reaction vessel, and the other end of which is fixedly connected to a storage tank. A grinding cylinder is rotatably connected to the inner side of the storage tank, and multiple sets of grinding blades are fixedly connected to the outer side of the grinding cylinder.

[0010] As a preferred embodiment of the above technical solution, a second synchronous pulley is fixedly connected to one end of the grinding cylinder, a second synchronous belt is rotatably connected to the outer side of the second synchronous pulley, a third synchronous pulley is rotatably connected to the inner side of the second synchronous belt, a first bevel gear is fixedly connected to one end of the third synchronous pulley via a rotating shaft, one end of the first bevel gear is rotatably connected to a storage box, a second bevel gear is meshed with the outer side of the first bevel gear, one end of the second bevel gear is rotatably connected to the storage box via a rotating shaft, a fourth synchronous pulley is fixedly connected to the other end of the second bevel gear, a third synchronous belt is rotatably connected to the outer side of the fourth synchronous pulley, and a fifth synchronous pulley is rotatably connected to the inner side of the third synchronous belt.

[0011] As a preferred embodiment of the above technical solution, the crushing mechanism includes a third gear, one end of which is fixedly connected to a fifth synchronous pulley, and the other end of which is rotatably connected to a connecting box. One side of the connecting box is fixedly connected to a storage box. A fourth gear is meshed with the outer side of the third gear, and the other end of the fourth gear is rotatably connected to the connecting box. Two sets of rollers are rotatably connected to the inner side of the connecting box. One end of one set of rollers is fixedly connected to the third gear, and one end of the other set of rollers is fixedly connected to the fourth gear. A second motor is installed at the front end of the connecting box, and the output shaft of the second motor is fixedly connected to the fourth gear.

[0012] Compared with the prior art, the beneficial effects of this utility model are: This device uses two sets of counter-rotating rollers for the first crushing, followed by a second crushing by multiple sets of grinding blades to form a slurry-like raw material. This dual crushing structure significantly improves the fineness of the raw material, providing a more sufficient reaction basis for subsequent enzymatic hydrolysis. The first gear drives three meshing sets of second gears, causing the first blade and multiple sets of second blades to rotate in tandem. The multi-dimensional stirring design ensures thorough mixing of materials, eliminates dead zones, and avoids excessive foaming caused by over-stirring, ensuring a stable reaction environment. Through the linkage of a temperature sensor and a PID controller, the temperature of the circulating coolant in the jacket can be precisely maintained at the optimal enzyme temperature. At the same time, the pH probe works in conjunction with the acid and alkali metering pumps to automatically adjust the pH of the system to the optimal range. This dual precise control maximizes enzyme activity and improves enzymatic hydrolysis efficiency. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of a low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis. Figure 2 A cross-sectional view of the overall structure of a low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis. Figure 1 ; Figure 3 A cross-sectional view of the overall structure of a low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis. Figure 2 ; Figure 4 A partial cross-sectional view of a low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis. Figure 1 ; Figure 5 A partial cross-sectional view of a low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis. Figure 2 ; Figure 6 This is a partial structural diagram of a low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis. Figure 7 A partial cross-sectional view of a low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis. Figure 3 ; Figure 8 This is a low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis. Figure 7 Enlarged schematic diagram of part A in the middle.

[0014] Legend: 1. Reaction mechanism; 11. Reaction vessel; 12. pH probe; 13. Acid metering pump; 14. Alkali metering pump; 15. PID controller; 16. Temperature sensor; 17. Jacket; 18. Circulating coolant; 19. Connecting pipe; 110. First gear; 111. First drive shaft; 112. First blade; 113. Second gear; 114. Second drive shaft; 115. Second blade; 116. First synchronous pulley; 117. First synchronous belt; 118. First... 1. Motor; 2. Partition plate; 3. Conveying mechanism; 4. Conveying pipe; 5. Storage box; 6. Grinding cylinder; 7. Grinding blade; 8. Second synchronous pulley; 9. Second synchronous belt; 10. Third synchronous pulley; 21. First bevel gear; 22. Second bevel gear; 23. Fourth synchronous pulley; 24. Third synchronous belt; 25. Fifth synchronous pulley; 36. Crushing mechanism; 37. Third gear; 38. Fourth gear; 39. Roller shaft; 30. Connecting box; 21. Second motor. Detailed Implementation

[0015] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.

[0016] Please see Figures 1-3 As shown, this utility model provides a technical solution: a low-temperature continuous reaction system for plant small molecule peptides based on compound enzymatic hydrolysis, including two sets of reaction mechanisms 1, a conveying mechanism 2 is provided on the outside of one set of reaction mechanisms 1, the conveying mechanism 2 includes a storage tank 22, and a crushing mechanism 3 is provided on the outside of the storage tank 22.

[0017] As one implementation method in this embodiment, please refer to Figures 4-5As shown, the reaction mechanism 1 includes a reaction vessel 11. A pH probe 12 is fixedly connected to the upper part of the inner side of the reaction vessel 11. An acid metering pump 13 is installed at the upper end of the reaction vessel 11 on one side of the pH probe 12, and an alkali metering pump 14 is installed at the upper end of the reaction vessel 11 on the other side of the pH probe 12. A jacket 17 is formed inside the reaction vessel 11. A circulating coolant 18 is fixedly connected to the inner side of the jacket 17. A temperature sensor 16 is fixedly connected to the front end of the reaction vessel 11 outside the circulating coolant 18. A PI pump is installed at the front end of the reaction vessel 11 on one side of the temperature sensor 16. The D controller 15 and the two sets of reaction vessels 11 are connected by a connecting pipe 19 through the interlayer 17. The two ends of the connecting pipe 19 are fixedly connected to the two sets of reaction vessels 11 respectively. The slurry plant raw material is transported to the upper end of the internal partition 119 of the reaction vessel 11. The temperature of the circulating coolant 18 in the interlayer 17 is monitored in real time by the temperature sensor 16 and linked with the PID controller 15 to ensure that the temperature of the reaction system is kept constant at the optimal temperature of the enzyme. The pH value inside is measured by the pH probe 12 at the upper end and works with the acid metering pump 13 and the alkali metering pump 14 to automatically maintain the optimal pH value of the reaction system.

[0018] As one implementation method in this embodiment, please refer to Figures 4-5 As shown, a first gear 110 is rotatably connected to the inner side of the reaction vessel 11 near its lower end. A first drive shaft 111 is fixedly connected to the inner side of the first gear 110. A partition 119 is rotatably connected to the outer side of the first drive shaft 111. Multiple sets of first blades 112 are fixedly connected to the outer side of the first drive shaft 111. Three sets of second gears 113 are meshed with the outer side of the first gear 110. One end of the second gear 113 is rotatably connected to the reaction vessel 11, and the other end of the second gear 113 is fixedly connected to a second drive shaft 114. The outer side of the second drive shaft 114 is rotatably connected to the partition plate 119. Multiple sets of second blades 115 are fixedly connected to the outer side of the second drive shaft 114. When the first gear 110 rotates, it further drives multiple sets of first blades 112 to rotate through the first drive shaft 111. When the first gear 110 rotates, it also drives three sets of meshing second gears 113 to rotate. The second gears 113 drive multiple sets of second blades 115 to rotate through the second drive shaft 114. The first blades 112 and the second blades 115 stir the reaction vessel 11.

[0019] As one implementation method in this embodiment, please refer to Figures 4-5As shown, a first synchronous pulley 116 is fixedly connected to the outer side of the reaction vessel 11 at the lower end of the first gear 110. The outer sides of the two sets of first synchronous pulleys 116 are rotatably connected to a first synchronous belt 117. A first motor 118 is installed at the lower end of a set of reaction vessels 11. The output shaft of the first motor 118 is fixedly connected to a set of first synchronous pulleys 116, driving the first motor 118 to rotate. Its output shaft drives a set of first synchronous pulleys 116 to rotate. The first synchronous pulleys 116 drive the first gear 110 to rotate. The first gear 110 in the next set is then driven to rotate through the first synchronous belt 117.

[0020] As one implementation method in this embodiment, please refer to Figures 6-7 As shown, the conveying mechanism 2 includes a conveying pipe 21. One end of the conveying pipe 21 is fixedly connected to the reaction container 11, and the other end of the conveying pipe 21 is fixedly connected to a storage box 22. A grinding cylinder 23 is rotatably connected to the inner side of the storage box 22, and multiple sets of grinding blades 24 are fixedly connected to the outer side of the grinding cylinder 23. The material is conveyed through the conveying pipe 21 to the inside of the reaction container 11 in the reaction mechanism 1. The grinding cylinder 23 and the multiple sets of grinding blades 24 rotate, and the grinding blades 24 perform secondary crushing on the plant material that slides into the storage box 22, making it into a slurry.

[0021] As one implementation method in this embodiment, please refer to Figures 6-8 As shown, a second synchronous pulley 25 is fixedly connected to one end of the grinding cylinder 23. A second synchronous belt 26 is rotatably connected to the outer side of the second synchronous pulley 25. A third synchronous pulley 27 is rotatably connected to the inner side of the second synchronous belt 26. A first bevel gear 28 is fixedly connected to one end of the third synchronous pulley 27 via a rotating shaft. One end of the first bevel gear 28 is rotatably connected to the storage box 22. A second bevel gear 29 is meshed with the outer side of the first bevel gear 28. One end of the second bevel gear 29 is rotatably connected to the storage box 22 via a rotating shaft. The other end of the second bevel gear 29 is fixedly connected to... The fourth synchronous pulley 210 is rotatably connected to the outer side of the fourth synchronous pulley 210 and the third synchronous belt 211 is rotatably connected to the inner side of the third synchronous belt 211. The fifth synchronous pulley 212 rotates and drives the fourth synchronous pulley 210 to rotate through the third synchronous belt 211. The fourth synchronous pulley 210 drives the second bevel gear 29 to rotate. The second bevel gear 29 drives the third synchronous pulley 27 to rotate through the meshing first bevel gear 28. The third synchronous pulley 27 drives the second synchronous pulley 25 to rotate through the second synchronous belt 26.

[0022] As one implementation method in this embodiment, please refer to Figures 6-7As shown, the crushing mechanism 3 includes a third gear 31, one end of which is fixedly connected to a fifth synchronous pulley 212, and the other end of which is rotatably connected to a connecting box 34. One side of the connecting box 34 is fixedly connected to a storage box 22. A fourth gear 32 is meshed with the outer side of the third gear 31, and the other end of the fourth gear 32 is rotatably connected to the connecting box 34. Two sets of rollers 33 are rotatably connected to the inner side of the connecting box 34. One end of one set of rollers 33 is fixedly connected to the third gear 31, and one end of the other set of rollers 33 is fixedly connected to the fourth gear 32. The connecting box 3... A second motor 35 is installed at the front end of 4. The output shaft of the second motor 35 is fixedly connected to the fourth gear 32. Plant materials and an appropriate amount of water or buffer solution are successively put into the upper end of the connecting box 34, driving the second motor 35 to run. Its output shaft drives the fourth gear 32 to rotate. The fourth gear 32 is driven to rotate through the meshing third gear 31. The third gear 31 and the fourth gear 32 rotate in opposite directions, driving the two sets of roller shafts 33 to rotate. The plant materials are crushed for the first time through the two sets of roller shafts 33. Then the plant materials slide down the inclined surface of the connecting box 34 into the storage box 22.

[0023] Working principle: During use, plant materials and an appropriate amount of water or buffer solution are successively placed into the upper end of the connecting box 34 in the crushing mechanism 3. The second motor 35 is driven to run, and its output shaft drives the fourth gear 32 to rotate. The fourth gear 32 drives the third gear 31 to rotate through its meshing. The third gear 31 and the fourth gear 32 rotate in opposite directions, driving the two sets of rollers 33 to rotate. The plant materials are crushed for the first time through the two sets of rollers 33. Then, the plant materials slide down the inclined surface of the connecting box 34 into the storage box 22 in the conveying mechanism 2. When the third gear 31 rotates, it drives the fifth synchronous pulley 212 to rotate. The fifth synchronous pulley 212 is connected to the third synchronous belt 21. 1 drives the fourth synchronous pulley 210 to rotate, the fourth synchronous pulley 210 drives the second bevel gear 29 to rotate, the second bevel gear 29 drives the third synchronous pulley 27 to rotate through the meshing first bevel gear 28, the third synchronous pulley 27 drives the second synchronous pulley 25 to rotate through the second synchronous belt 26, the second synchronous pulley 25 drives the grinding cylinder 23 and multiple sets of grinding blades 24 to rotate, the grinding blades 24 perform secondary crushing of the plant material that slides into the storage box 22, making it into a slurry, the slurry plant material slides down the grinding cylinder 23 and is squeezed into the conveying pipe 21 when the grinding cylinder 23 rotates, and is conveyed through the conveying pipe 21 to the internal partition 11 of the reaction vessel 11 in a set of reaction mechanisms 1. The upper end of 9 then drives the first motor 118 to operate. Its output shaft drives a set of first synchronous pulleys 116 to rotate. The first synchronous pulleys 116 drive the first gear 110 to rotate. Through the first transmission shaft 111, the first gear 112 is further driven to rotate. When the first gear 110 rotates, it also drives the three meshing sets of second gears 113 to rotate. The second gears 113 drive the multiple sets of second blades 115 to rotate through the second transmission shaft 114. Through the stirring of the first blades 112 and the second blades 115 in the reaction vessel 11, the materials are fully mixed without dead zones, while avoiding the generation of excessive foam. The temperature sensor 16 monitors the contents of the jacket 17 in real time. The temperature of the circulating coolant 18 is linked with the PID controller 15 to ensure that the reaction system temperature is kept constant at the optimal enzyme temperature. The pH value inside is measured by the pH probe 12 at the top. In conjunction with the acid metering pump 13 and the alkali metering pump 14, the optimal pH value of the reaction system is automatically maintained. The calculated amount of enzyme preparation is dissolved and diluted with a small amount of warm water or buffer solution and then added evenly to the raw material slurry with the temperature and pH already adjusted under stirring. The mixture after reaction is then introduced into the next set of reaction vessels 11 through the connecting pipe 19. The first gear 110 in the next set is driven to rotate by the first synchronous belt 117, thereby efficiently extracting small molecule peptides through enzymatic hydrolysis.

[0024] The above embodiments are only used to illustrate the technical solution of this utility model, and are not intended to limit it.

Claims

1. A low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis, comprising two sets of reaction mechanisms (1), characterized in that: A conveying mechanism (2) is provided on the outside of a set of reaction mechanisms (1). The conveying mechanism (2) includes a storage box (22). A crushing mechanism (3) is provided on the outside of the storage box (22).

2. The low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis according to claim 1, characterized in that: The reaction mechanism (1) includes a reaction container (11). A pH probe (12) is fixedly connected to the upper part of the inner side of the reaction container (11). An acid metering pump (13) is provided at the upper end of the reaction container (11) on one side of the pH probe (12), and an alkali metering pump (14) is provided at the upper end of the reaction container (11) on the other side of the pH probe (12). A jacket (17) is provided on the inner side of the reaction container (11). A circulating coolant (18) is fixedly connected to the inner side of the jacket (17). A temperature sensor (16) is fixedly connected to the front end of the reaction container (11) on the outer side of the circulating coolant (18). A PID controller (15) is provided at the front end of the reaction container (11) on one side of the temperature sensor (16). A connecting pipe (19) is provided between the two sets of reaction containers (11) through the jacket (17). The two ends of the connecting pipe (19) are fixedly connected to the two sets of reaction containers (11) respectively.

3. The low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis according to claim 2, characterized in that: The reaction vessel (11) is rotatably connected to a first gear (110) near its lower end. The inner side of the first gear (110) is fixedly connected to a first drive shaft (111). The outer side of the first drive shaft (111) is rotatably connected to a partition (119). Multiple sets of first blades (112) are fixedly connected to the outer side of the first drive shaft (111). Three sets of second gears (113) are meshed with the outer side of the first gear (110). One end of the second gear (113) is rotatably connected to the reaction vessel (11). The other end of the second gear (113) is fixedly connected to a second drive shaft (114). The outer side of the second drive shaft (114) is rotatably connected to the partition (119). Multiple sets of second blades (115) are fixedly connected to the outer side of the second drive shaft (114).

4. The low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis according to claim 3, characterized in that: The outer side of the reaction vessel (11) at the lower end of the first gear (110) is fixedly connected to a first synchronous pulley (116). The outer sides of the two sets of first synchronous pulleys (116) are rotatably connected to a first synchronous belt (117). A first motor (118) is installed at the lower end of one set of the reaction vessels (11). The output shaft of the first motor (118) is fixedly connected to a set of first synchronous pulleys (116).

5. The low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis according to claim 1, characterized in that: The conveying mechanism (2) includes a conveying pipe (21), one end of which is fixedly connected to the reaction vessel (11), and the other end of which is fixedly connected to a storage tank (22). A grinding cylinder (23) is rotatably connected to the inner side of the storage tank (22), and multiple sets of grinding blades (24) are fixedly connected to the outer side of the grinding cylinder (23).

6. The low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis according to claim 5, characterized in that: One end of the grinding cylinder (23) is fixedly connected to a second synchronous pulley (25). The outer side of the second synchronous pulley (25) is rotatably connected to a second synchronous belt (26). The inner side of the second synchronous belt (26) is rotatably connected to a third synchronous pulley (27). One end of the third synchronous pulley (27) is fixedly connected to a first bevel gear (28) via a rotating shaft. One end of the first bevel gear (28) is rotatably connected to a storage box (22). The outer side of the first bevel gear (28) is meshed with a second bevel gear (29). One end of the second bevel gear (29) is rotatably connected to the storage box (22) via a rotating shaft. The other end of the second bevel gear (29) is fixedly connected to a fourth synchronous pulley (210). The outer side of the fourth synchronous pulley (210) is rotatably connected to a third synchronous belt (211). The inner side of the third synchronous belt (211) is rotatably connected to a fifth synchronous pulley (212).

7. The low-temperature continuous reaction system for plant small molecule peptides based on complex enzymatic hydrolysis according to claim 1, characterized in that: The crushing mechanism (3) includes a third gear (31), one end of which is fixedly connected to a fifth synchronous wheel (212), and the other end of which is rotatably connected to a connecting box (34). One side of the connecting box (34) is fixedly connected to a storage box (22). A fourth gear (32) is meshed with the outer side of the third gear (31), and the other end of the fourth gear (32) is rotatably connected to the connecting box (34). Two sets of rollers (33) are rotatably connected to the inner side of the connecting box (34). One end of one set of rollers (33) is fixedly connected to the third gear (31), and one end of the other set of rollers (33) is fixedly connected to the fourth gear (32). A second motor (35) is installed at the front end of the connecting box (34), and the output shaft of the second motor (35) is fixedly connected to the fourth gear (32).

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