An apparatus for inducing emt of synovial sarcoma cells
Patent Information
- Application Number
- CN202522641212.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-12
- Publication Date
- 2026-11-03
- Estimated Expiration
- 2035-12-12
AI Technical Summary
In existing EMT induction experimental devices, uneven distribution of inducing factors leads to large differences in cell induction effects, affecting experimental reproducibility. Furthermore, the lack of temperature control results in fluctuations in the culture environment, affecting cell activity and induction efficiency.
An experimental device for inducing EMT in synovial sarcoma cells was designed, comprising an adjustable support structure, a heating element, a stirring assembly, and an inducing factor supply structure. The stirring assembly evenly distributes the inducing factor, the heating element controls the temperature, and a transparent borosilicate glass culture tube facilitates observation.
It achieves uniform distribution of inducing factors in the culture medium, improves experimental repeatability, maintains suitable temperature, reduces the impact of temperature fluctuations on cell activity, and facilitates observation and recording of the experimental process.
Smart Images
Figure CN224930577U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biomedical experimental equipment technology, specifically to an experimental device for inducing EMT in synovial sarcoma cells. Background Technology
[0002] In synovial sarcoma cell EMT induction experiments, EMT refers to Epithelial-Mesenchymal Transition, or EMT for short, the process by which epithelial cells transform into mesenchymal cells through a specific procedure. This process plays a crucial role in tumor invasion, metastasis, and tissue repair. Key characteristics: EMT leads to changes in cell morphology (e.g., epithelial cells becoming spindle-shaped), loss of cell polarity, and weakened intercellular adhesion, while simultaneously granting the cells the ability to migrate and invade. In tumor research, it is considered one of the key mechanisms of cancer cell spread. Experimental applications: By inducing EMT in synovial sarcoma cells, the tumor metastasis process can be simulated, providing an experimental model for studying anticancer drug targets. For example, inhibiting EMT-related genes or signaling pathways (such as transcription factors like Snail and Twist) may suppress tumor metastasis.
[0003] In synovial sarcoma research, the EMT process is closely related to tumor invasion, metastasis, and drug resistance. Therefore, EMT induction experiments on synovial sarcoma cells are an important step in exploring its pathogenesis and treatment strategies. Currently, most commonly used EMT induction experimental devices in laboratories consist of ordinary cell culture dishes combined with external inducing factor addition equipment, which has the following shortcomings: I. The inducing factors are easily unevenly distributed in the culture system, resulting in large differences in the induction effect of some cells, which affects the reproducibility of the experiment; Second, there is a lack of temperature control in the culture environment, and fluctuations in culture temperature directly affect the activity of synovial sarcoma cells and the efficiency of EMT induction. Based on this, an experimental device for inducing EMT in synovial sarcoma cells is proposed. Utility Model Content
[0004] This invention aims to solve the problems mentioned in the background art by providing an experimental device for inducing EMT in synovial sarcoma cells, thereby addressing: I. The inducing factors are easily unevenly distributed in the culture system, resulting in large differences in the induction effect of some cells, which affects the reproducibility of the experiment; Second, there is a lack of temperature control in the culture environment, and fluctuations in culture temperature directly affect the activity of synovial sarcoma cells and the efficiency of EMT induction.
[0005] The specific technical solution is as follows: An experimental apparatus for inducing EMT in synovial sarcoma cells, comprising: The base has a circular mounting groove on its upper surface; An adjustable support structure is mounted on the base; A heating element is installed within the circular mounting groove; A culture tube is detachably installed in the circular mounting slot, and after installation, the bottom of the culture tube is in contact with the heating element; A stirring assembly, one end of which is mounted on the adjustable support structure, can move up and down with the adjustable support structure. During downward movement, the other end connects to the culture tube, while during upward movement, the culture tube can be disassembled. The inducing factor supply structure has one end mounted on the adjustable support structure and the other end mounted on the culture tube.
[0006] As a preferred embodiment of the present invention, the base has a connecting part in the middle of one side wall, and the connecting part is integrally formed with the base; The adjustable support structure includes a support rod, which is fixedly installed on the upper surface of the connecting part, and a T-shaped sliding groove is provided on the support rod; A support frame is installed at one top end of the support rod, and a T-shaped slide bar is fixedly installed at the bottom of the support frame at the corresponding T-shaped slide groove. The T-shaped slide bar is slidably installed in the T-shaped slide groove. The support frame has a circular support portion at the end away from the support rod; The adjustable support structure also includes an adjustment component, one end of which is connected to the connecting part, and the other end is connected to the T-shaped slide bar.
[0007] In a preferred embodiment of this utility model, the adjusting component includes a connecting block and an electric cylinder. The connecting block is fixedly installed at the bottom end of the outer wall of the T-shaped slide bar, the cylinder barrel of the electric cylinder is fixedly installed on the upper surface of the connecting part, and the piston rod end of the electric cylinder is fixedly installed on the connecting block by a fixing ring.
[0008] In a preferred embodiment of this utility model, the heating element is a heating coil, which is fixedly installed in a circular mounting groove. The culture tube is embedded in a circular mounting groove; When the heating coil is heated, its heat can be conducted to the culture tube.
[0009] In a preferred embodiment of this utility model, the position of the circular support part corresponds to the position of the center of the culture tube; An assembly hole A is provided at the center of the circular support portion; A shaft hole is provided at the center of the bottom of the inner cavity of the culture tube; The shaft hole and the assembly hole A are concentric and coaxial, and have the same shape and size; The stirring assembly includes a stepper motor, which is fixedly mounted on the upper surface of the circular support. The output shaft of the stepper motor extends downward through the assembly hole A. A shaft is fixedly mounted at the end of the output shaft of the stepper motor, and one bottom end of the shaft is rotatably mounted in the shaft hole. The stirring assembly also includes a stirring frame, the central part of which has a bushing, and the bushing of the stirring frame is fixedly installed at the bottom end of the shaft. The stirring assembly also includes a cylinder cover, which has a mounting hole at its center, and the cylinder cover is fixedly mounted on the shaft through the mounting hole; When the stirring assembly moves downward and the stirring rack is inside the culture tube, the tube cover can close the top opening of the culture tube.
[0010] As a preferred embodiment of this utility model, the stirring rack has multiple sets of vertically arranged and parallel stirring fins.
[0011] As a preferred embodiment of this utility model, an assembly hole B is provided on one side of the top of the support frame; The culture tube has a liquid inlet on the upper part of its side wall; The inducing factor supply structure includes an inducing factor storage tank, which is fixedly installed on the upper surface of the support frame. The top side of the inducing factor storage tank has a feeding pipe, and a sealing plug is inserted into the feeding pipe. The bottom of the inducing factor storage tank has a discharge pipe, which extends downward through the assembly hole B; A discharge regulating valve is fixedly installed on the discharge pipe; The discharge pipe is connected to a feed hose, the free end of which is inserted into the liquid inlet and connected to the inner cavity of the culture tube.
[0012] As a preferred embodiment of this utility model, the cylinder cap is provided with an openable and closable culture medium addition structure.
[0013] As a preferred embodiment of the present invention, the openable culture medium addition structure includes a filling port opened at the edge of the cylinder cover and a circular cover plate movably installed at the filling port. The circular cover plate has an arc-shaped connecting part at its edge, which is integrally formed with the circular cover plate. The arc-shaped connecting part is connected to the upper surface of the cylindrical cover by a pin. The circular cover plate can move in a circular motion around the pin on the arc-shaped connecting part to open or close the feeding port; The circular cover plate is larger than the filling port, and when the circular cover plate is rotated to the filling port, it can completely cover the filling port.
[0014] As a preferred embodiment of this utility model, when the circular cover plate closes the filling port, a locking structure is also installed between the circular cover plate and the cylindrical cover. The locking structure includes a locking hole A located at the outer edge of the filling port and a locking hole B located at the edge of the circular cover plate and corresponding to the position of the locking hole A. The shape and size of the locking hole A are matched with the shape and size of the locking hole B; When the locking hole B coincides with the locking hole A, a locking pin is inserted into the locking hole B and the locking hole A. After the locking pin is inserted, the circular cover plate is locked and fixed.
[0015] This utility model has the following beneficial effects: I. In this utility model, the stirring component can make the inducing factor solution evenly distributed in the culture medium in the culture tube, avoiding large differences in cell induction effect due to uneven distribution of inducing factors, and improving the repeatability of the experiment.
[0016] Second, in this utility model, the heating element (heating coil) can regulate the temperature of the culture environment inside the culture tube, maintain a suitable temperature, and reduce the adverse effects of temperature fluctuations on the activity of synovial sarcoma cells and the efficiency of EMT induction.
[0017] Third, in this invention, the culture tube is made of transparent borosilicate glass, which facilitates external observation of the internal synovial sarcoma cells and the induction process, and is beneficial for experimental observation and recording.
[0018] Fourth, in this utility model, the adjustable support structure facilitates the adjustment of the position of each component, enabling precise docking or disassembly of the stirring assembly, the induction factor supply structure, and the culture tube, making operation convenient.
[0019] Fifth, the openable and closed culture medium addition structure and locking structure of this utility model facilitate the addition of culture medium during the experiment and can effectively seal the environment to ensure the stability of the culture environment. Attached Figure Description
[0020] Figure 1 This is a three-dimensional schematic diagram of a partial cross-section of the present invention; Figure 2 This is a three-dimensional schematic diagram of the present invention; Figure 3 This is a three-dimensional schematic diagram of the adjustable support structure and heating element of this utility model. Figure 4This is a three-dimensional schematic diagram of the present invention viewed from below; Figure 5 This is a three-dimensional schematic diagram of the support rod of this utility model; Figure 6 An exploded view of the combination of the cap and the opening / closing culture medium addition structure and locking structure of this utility model; Figure 7 This is a three-dimensional schematic diagram of the culture tube of this utility model.
[0021] In the picture: 10. Base; 101. Connecting part; 102. Circular insert groove; 20. Support rod; 201. T-shaped slide groove; 30. Support frame; 301. Circular support part; 302. Assembly hole A; 303. Assembly hole B; 40. T-shaped slide bar; 50. Connecting block; 60. Electric cylinder; 70. Heating coil; 80. Culture cylinder; 801. Shaft hole; 802. Liquid inlet; 90. Stepper motor; 100. Shaft; 110 1101. Mixing rack; 120. Mixing fins; 120. Cylinder cover; 1201. Mounting hole; 1202. Feeding port; 1203. Locking hole A; 130. Circular cover plate; 1301. Arc-shaped connection; 1302. Locking hole B; 140. Locking pin; 150. Inducing factor storage tank; 1501. Feeding pipe; 1502. Discharge pipe; 160. Sealing plug; 170. Discharge regulating valve; 180. Feeding hose. Detailed Implementation
[0022] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments.
[0023] The accompanying drawings are for illustrative purposes only and are schematic diagrams, not actual images. They should not be construed as limiting the scope of this patent. To better illustrate the embodiments of this utility model, some components in the drawings may be omitted, enlarged, or reduced, and do not represent the actual dimensions of the product. It is understandable to those skilled in the art that some well-known structures and their descriptions may be omitted in the drawings.
[0024] In the accompanying drawings of this utility model, the same or similar reference numerals correspond to the same or similar components. In the description of this utility model, it should be understood that if terms such as "upper," "lower," "left," "right," "inner," and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the drawings are only for illustrative purposes and should not be construed as limiting this patent. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.
[0025] In the description of this utility model, unless otherwise explicitly specified and limited, the term "connection" or similar designation indicating a connection relationship between components should be interpreted broadly. For example, it can refer to a fixed connection, a detachable connection, or an integral part; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can refer to the internal communication between two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances. Currently, most commonly used EMT induction experimental devices in laboratories consist of ordinary cell culture dishes combined with external inducing factor addition equipment, which has the following shortcomings: I. The inducing factors are easily unevenly distributed in the culture system, resulting in large differences in the induction effect of some cells, which affects the reproducibility of the experiment; Second, there is a lack of temperature control in the culture environment, and fluctuations in culture temperature directly affect the activity of synovial sarcoma cells and the efficiency of EMT induction. Based on this, the following technical solution is proposed, as detailed below: Example 1
[0026] like Figures 1-7 As shown in the figure, an EMT induction experimental device for synovial sarcoma cells provided by this utility model includes: The base 10 has a circular mounting groove 102 on its upper surface; An adjustable support structure is mounted on the base 10; A heating element is installed in a circular mounting groove 102; Culture tube 80 is used for induction experiments of synovial sarcoma cells. During the induction experiment, the inducing factor solution is added to the culture medium of synovial sarcoma cells. The culture tube 80 is detachably installed in the circular insert 102, and after installation, the bottom of the culture tube 80 is in contact with the heating element. Before the induction experiment, the culture medium containing synovial sarcoma cells can be added to the culture tube 80; The culture tube 80 is made of transparent borosilicate glass, which allows for easy external observation. The stirring assembly has one end mounted on an adjustable support structure, allowing it to move up and down with the structure. During downward movement, the other end engages with the culture tank 80; during upward movement, the culture tank 80 can be disassembled. The inducing factor supply structure contains an inducing factor solution. One end is mounted on an adjustable support structure, and the other end is mounted on a culture tube 80.
[0027] In this embodiment, the circular mounting groove 102 on the base 10 is used to install the heating element and the culture cylinder 80; the adjustable support structure is used to support the stirring assembly and the inducing factor supply structure, and can adjust their vertical position; the heating element is installed in the circular mounting groove 102 to heat the culture cylinder 80; the culture cylinder 80 is made of transparent borosilicate glass and is used to contain the culture medium containing synovial sarcoma cells, and is detachably installed in the circular mounting groove 102, with its bottom in contact with the heating element to receive heat after installation; one end of the stirring assembly is installed on the adjustable support structure, and moves up and down with it, docking with the culture cylinder 80 when it is down, and being detached from the culture cylinder 80 when it is up; the inducing factor supply structure contains an inducing factor solution, with one end installed on the adjustable support structure and the other end installed on the culture cylinder 80, for providing the inducing factor solution to the culture cylinder 80. Example 2
[0028] like Figures 1-7 As shown, the improvements are based on Example 1: Furthermore, the base 10 has a connecting part 101 in the middle of one side wall, and the connecting part 101 is integrally formed with the base 10; The adjustable support structure includes a support rod 20, which is fixedly installed on the upper surface of the connecting part 101, and a T-shaped groove 201 is provided on the support rod 20; A support frame 30 is installed at the top end of the support rod 20, and a T-shaped slide bar 40 is fixedly installed at the bottom of the support frame 30 at the corresponding T-shaped slide groove 201. The T-shaped slide bar 40 is slidably installed in the T-shaped slide groove 201. The support frame 30 has a circular support portion 301 at the end away from the support rod 20; The adjustable support structure also includes an adjustment component, one end of which is connected to the connecting part 101, and the other end is connected to the T-shaped slide bar 40.
[0029] Furthermore, the adjusting components include a connecting block 50 and an electric cylinder 60. The connecting block 50 is fixedly installed at the bottom end of the outer wall of the T-shaped slide bar 40, the cylinder barrel of the electric cylinder 60 is fixedly installed on the upper surface of the connecting part 101, and the piston rod end of the electric cylinder 60 is fixedly installed on the connecting block 50 by a fixing ring.
[0030] In this embodiment, the connecting portion 101 of the base 10 is used to mount the support rod 20. The T-shaped groove 201 on the support rod 20 cooperates with the T-shaped slide bar 40 at the bottom of the support frame 30, allowing the support frame 30 to slide along the support rod 20. The cylinder 60 of the adjusting component has its cylinder barrel fixed to the upper surface of the connecting portion 101, and its piston rod is connected to the connecting block 50 through a fixing ring. The connecting block 50 is fixed to the T-shaped slide bar 40. When the electric cylinder 60 operates, it can push the T-shaped slide bar 40 to slide along the T-shaped groove 201, thereby adjusting the position of the support frame 30.
[0031] Furthermore, the heating element is a heating coil 70, which is fixedly installed in the circular mounting groove 102; The heating coil 70 is flat; The culture tube 80 is embedded in the circular mounting groove 102; When the heating coil 70 is heated, its heat can be conducted to the culture tube 80.
[0032] In this embodiment, the heating coil 70 is fixed in the circular insert groove 102 and is flat. The culture tube 80 is embedded in the circular insert groove 102. When the heating coil 70 is heated, the heat is conducted to the culture tube 80 to provide temperature for the internal cell culture.
[0033] Furthermore, the position of the circular support 301 corresponds to the position of the center of the culture tube 80; An assembly hole A302 is provided at the center of the circular support part 301; A shaft hole 801 is provided at the center of the bottom of the inner cavity of the culture tube 80; Shaft hole 801 and assembly hole A302 are concentric and coaxial, and have the same shape and size; The stirring assembly includes a stepper motor 90, which is fixedly mounted on the upper surface of the circular support 301. The output shaft of the stepper motor 90 extends downward through the mounting hole A302. A shaft 100 is fixedly mounted on the end of the output shaft of the stepper motor 90, and the bottom end of the shaft 100 is rotatably mounted in the shaft hole 801. The mixing assembly also includes a mixing frame 110, which has a bushing at its center and is fixedly installed at the bottom end of the shaft 100. The stirring assembly also includes a cylinder cover 120, which has a mounting hole 1201 at its center. The cylinder cover 120 is fixedly mounted on the shaft 100 through the mounting hole 1201. When the stirring assembly moves downward and the stirring rack 110 is inside the culture tube 80, the tube cover 120 can close the top opening of the culture tube 80.
[0034] Furthermore, the stirring rack 110 has multiple sets of vertically arranged and parallel stirring fins 1101.
[0035] In this embodiment, the mounting hole A302 at the center of the circular support 301 is concentric and coaxial with the shaft hole 801 at the bottom of the culture cylinder 80, and has the same size and shape. It is used to install the output shaft and shaft 100 of the stepper motor 90. The stepper motor 90 is mounted on the upper surface of the circular support 301, with its output shaft passing through the mounting hole A302 and the shaft 100 fixed at its end. The bottom of the shaft 100 is rotatably mounted in the shaft hole 801. The stirring frame 110 is fixed to the bottom of the shaft 100 by a bushing, and the cylinder cover 120 is fixed to the shaft 100 by the mounting hole 1201. When the stirring assembly moves downward and the stirring frame 110 is inside the culture cylinder 80, the cylinder cover 120 can close the top opening of the culture cylinder 80. The multiple sets of vertical parallel stirring fins 1101 on the stirring frame 110 rotate under the drive of the shaft 100, realizing the stirring of the material inside the culture cylinder 80.
[0036] Furthermore, an assembly hole B303 is provided on one side of the top of the support frame 30; A liquid inlet 802 is provided on the upper part of the side wall of the culture tube 80; The inducing factor supply structure includes an inducing factor storage tank 150, which is fixedly installed on the upper surface of the support frame 30. The top side of the inducing factor storage tank 150 has a feeding pipe 1501, and a sealing plug 160 is inserted into the feeding pipe 1501. After the sealing plug 160 is pulled out, the inducing factor solution can be added to the inducing factor storage tank 150 through the feeding pipe 1501; The bottom of the inducing factor storage tank 150 has a discharge pipe 1502, which extends downward through the assembly hole B303; A discharge regulating valve 170 is fixedly installed on the discharge pipe 1502; A feed hose 180 is connected to the discharge pipe 1502. The free end of the feed hose 180 is inserted into the liquid inlet 802 and connected to the inner cavity of the culture tube 80. By opening the discharge regulating valve 170, the inducing factor solution in the inducing factor storage tank 150 can flow into the culture tube 80 to participate in the induction experiment of synovial sarcoma cells.
[0037] In this embodiment, the mounting hole B303 on the top of the support frame 30 is used to install the discharge pipe 1502 of the inducing factor storage tank 150. The inducing factor solution in the inducing factor storage tank 150 can be added through the feeding pipe 1501 (by pulling out the sealing plug 160). The discharge regulating valve 170 on the discharge pipe 1502 controls the discharge of the solution. The feeding hose 180 connects the discharge pipe 1502 to the inlet 802 of the culture tube 80. By opening the discharge regulating valve 170, the inducing factor solution can flow into the culture tube 80 through the feeding hose 180.
[0038] Example 3 like Figures 1-7 As shown, the improvements are based on Example 2: Furthermore, the cap 120 is equipped with an openable and closed culture medium addition structure, through which culture medium for synovial sarcoma cells can be added into the culture tube 80.
[0039] Furthermore, the open-closed culture medium addition structure includes a filling port 1202 opened at the edge of the cylinder cover 120 and a circular cover plate 130 movably installed at the filling port 1202. The circular cover plate 130 has an arc-shaped connecting part 1301 at its edge. The arc-shaped connecting part 1301 is integrally formed with the circular cover plate 130. The arc-shaped connecting part 1301 is connected to the upper surface of the cylindrical cover 120 by a pin. The circular cover plate 130 can move in a circular motion around the pin on the arc-shaped connecting part 1301 to open or close the feeding port 1202; The circular cover plate 130 is larger than the feed port 1202. When the circular cover plate 130 is rotated to the feed port 1202, the circular cover plate 130 can completely cover the feed port 1202.
[0040] In this embodiment, the openable culture medium addition structure on the cylinder cover 120 adds culture medium through the filling port 1202 and the circular cover plate 130. The arc-shaped connecting part 1301 of the circular cover plate 130 is connected to the cylinder cover 120 by a pin. The circular cover plate 130 can move around the pin to open the filling port 1202 to add culture medium. When rotated to the filling port 1202, the circular cover plate 130, being larger than the filling port 1202, can completely cover the filling port 1202.
[0041] Example 4 like Figures 1-7 As shown, the improvements are based on Example 3: Furthermore, when the circular cover plate 130 covers the feeding port 1202, a locking structure is also installed between the circular cover plate 130 and the cylindrical cover 120. The locking structure includes a locking hole A1203 opened at the outer edge of the filling port 1202 and a locking hole B1302 opened at the edge of the circular cover plate 130 and corresponding to the position of the locking hole A1203. The shape and size of the locking hole A1203 match the shape and size of the locking hole B1302. When the locking hole B1302 coincides with the locking hole A1203, a locking pin 140 is inserted into the locking hole B1302 and the locking hole A1203. After the locking pin 140 is inserted, it locks and fixes the circular cover plate 130.
[0042] In this embodiment, when the circular cover plate 130 covers the filling port 1202, the locking hole A1203 of the locking structure coincides with the locking hole B1302. By inserting the locking pin 140 into the overlapping locking holes A1203 and B1302, the circular cover plate 130 can be locked and fixed on the cylinder cover 120.
[0043] The different embodiments described above can be combined, substituted, or used in combination with each other.
[0044] Work steps: like Figures 1-7 As shown, a support rod 20 is installed on the connecting part 101 of the base 10. The support rod 20 engages with the T-shaped slide bar 40 of the support frame 30 via a T-shaped slide groove 201. The electric cylinder 60 drives the T-shaped slide bar 40 to slide and adjust the position of the support frame 30. A heating coil 70 is installed in the circular mounting groove 102 of the base 10 to heat the culture cylinder 80 embedded therein. The culture cylinder 80 is used to contain culture medium containing synovial sarcoma cells. The cylinder cover 120 can cover its top. The filling port 1202 on the cylinder cover 120 engages with the circular cover plate 130 and the locking pin 140 to realize the addition and sealing of the culture medium. A stepper motor 90 is installed on the circular support part 301 of the support frame 30 and drives the stirring frame 110 to rotate via the shaft 100 to stir the material in the culture cylinder 80. The inducing factor storage tank 150 is installed on the support frame 30. The inducing factor solution inside flows into the culture cylinder 80 through the discharge regulating valve 170 and the feeding hose 180 to participate in the EMT induction experiment of synovial sarcoma cells.
[0045] In summary: First, the stirring assembly ensures that the inducing factor solution is evenly distributed in the culture medium within the culture tube 80, avoiding large differences in cell induction effects due to uneven distribution of the inducing factor and improving experimental repeatability.
[0046] Second, the heating element (heating coil 70) can regulate the temperature of the culture environment inside the culture tube 80, maintain a suitable temperature, and reduce the adverse effects of temperature fluctuations on the activity of synovial sarcoma cells and the efficiency of EMT induction.
[0047] Third, the culture tube 80 is made of transparent borosilicate glass, which facilitates external observation of the internal synovial sarcoma cells and the induction process, and is beneficial for experimental observation and recording.
[0048] IV. The adjustable support structure allows for easy adjustment of the position of each component, enabling precise docking or disassembly of the stirring assembly, induction factor supply structure, and culture tube 80, making operation convenient.
[0049] V. The open and closed culture medium addition structure and locking structure facilitate the addition of culture medium during the experiment and can effectively seal the environment to ensure the stability of the culture environment.
[0050] The above are merely preferred embodiments of the present utility model and are not intended to limit the implementation methods and protection scope of the present utility model. Those skilled in the art should realize that any equivalent substitutions and obvious changes made based on the description and illustrations of the present utility model should be included within the protection scope of the present utility model.
Claims
1. An experimental apparatus for inducing EMT in synovial sarcoma cells, characterized in that, include: The base (10) has a circular mounting groove (102) on its upper surface; An adjustable support structure is mounted on the base (10); A heating element is installed in the circular mounting groove (102); The culture tube (80) is detachably installed in the circular insert (102), and after installation, the bottom of the culture tube (80) is in contact with the heating element; The stirring assembly has one end installed on the adjustable support structure and can move up and down with the adjustable support structure. During the downward movement, the other end is connected to the culture tube (80) and installed. During the upward movement, the culture tube (80) can be disassembled. as well as The inducing factor supply structure has one end mounted on the adjustable support structure and the other end mounted on the culture tube (80).
2. The experimental apparatus for inducing EMT in synovial sarcoma cells according to claim 1, characterized in that, The base (10) has a connecting part (101) in the middle of one side wall, and the connecting part (101) is integrally formed with the base (10); The adjustable support structure includes a support rod (20), which is fixedly installed on the upper surface of the connecting part (101), and a T-shaped groove (201) is provided on the support rod (20). A support frame (30) is installed at one end of the top of the support rod (20), and a T-shaped slide bar (40) is fixedly installed at the bottom of the support frame (30) at the corresponding T-shaped slide groove (201). The T-shaped slide bar (40) is slidably installed in the T-shaped slide groove (201). The support frame (30) has a circular support portion (301) at the end away from the support rod (20). The adjustable support structure also includes an adjustment component, one end of which is connected to the connecting part (101), and the other end is connected to the T-shaped slide bar (40).
3. The experimental apparatus for inducing EMT in synovial sarcoma cells according to claim 2, characterized in that, The adjusting component includes a connecting block (50) and an electric cylinder (60). The connecting block (50) is fixedly installed at the bottom end of the outer wall of the T-shaped slide bar (40). The cylinder barrel of the electric cylinder (60) is fixedly installed on the upper surface of the connecting part (101). The piston rod end of the electric cylinder (60) is fixedly installed on the connecting block (50) by a fixing ring.
4. The experimental apparatus for inducing EMT in synovial sarcoma cells according to claim 2, characterized in that, The heating element is a heating coil (70), which is fixedly installed in a circular mounting groove (102); The culture tube (80) is fitted into the circular fitting groove (102); When the heating coil (70) is heated, its heat can be conducted to the culture tube (80).
5. The experimental apparatus for inducing EMT in synovial sarcoma cells according to claim 2 or 4, characterized in that, The position of the circular support (301) corresponds to the position of the center of the culture tube (80); An assembly hole A (302) is provided at the center of the circular support part (301); A shaft hole (801) is provided at the center of the bottom of the inner cavity of the culture tube (80). The shaft hole (801) and the assembly hole A (302) are concentric and coaxial, and have the same shape and size; The stirring assembly includes a stepper motor (90), which is fixedly mounted on the upper surface of the circular support (301). The output shaft of the stepper motor (90) passes downward through the mounting hole A (302). A shaft (100) is fixedly mounted at the end of the output shaft of the stepper motor (90), and one bottom end of the shaft (100) is rotatably mounted in the shaft hole (801). The stirring assembly also includes a stirring frame (110), the stirring frame (110) has a bushing at its center, and the bushing of the stirring frame (110) is fixedly installed at the bottom end of the shaft (100). The stirring assembly also includes a cylinder cover (120), which has a mounting hole (1201) at its center. The cylinder cover (120) is fixedly mounted on the shaft (100) through the mounting hole (1201). When the stirring assembly moves downward and the stirring rack (110) is inside the cavity of the culture tube (80), the tube cover (120) can close the top opening of the culture tube (80).
6. The experimental apparatus for inducing EMT in synovial sarcoma cells according to claim 5, characterized in that, The stirring rack (110) has multiple sets of vertically arranged and parallel stirring fins (1101).
7. The experimental apparatus for inducing EMT in synovial sarcoma cells according to claim 2, characterized in that, The support frame (30) has an assembly hole B (303) on one side of its top. The culture tube (80) has a liquid inlet (802) on the upper part of its side wall. The inducing factor supply structure includes an inducing factor storage tank (150), which is fixedly installed on the upper surface of the support frame (30). The top side of the inducing factor storage tank (150) has a feeding pipe (1501), and a sealing plug (160) is inserted into the feeding pipe (1501). The bottom of the inducing factor storage tank (150) has a discharge pipe (1502), which extends downward through the assembly hole B (303); A discharge regulating valve (170) is fixedly installed on the discharge pipe (1502); The discharge pipe (1502) is connected to a feed hose (180), the free end of which is inserted into the liquid inlet (802) and connected to the inner cavity of the culture tube (80).
8. The experimental apparatus for inducing EMT in synovial sarcoma cells according to claim 5, characterized in that, The cap (120) is provided with an openable culture medium addition structure.
9. The experimental apparatus for inducing EMT in synovial sarcoma cells according to claim 8, characterized in that, The openable culture medium addition structure includes a filling port (1202) opened at the edge of the cylinder cover (120) and a circular cover plate (130) movably installed at the filling port (1202). The circular cover plate (130) has an arc-shaped connecting part (1301) at its edge. The arc-shaped connecting part (1301) is integrally formed with the circular cover plate (130). The arc-shaped connecting part (1301) is connected to the upper surface of the cylindrical cover (120) by a pin. The circular cover plate (130) can move in a circular motion around the pin on the arc-shaped connecting part (1301) to open or close the feeding port (1202); The circular cover plate (130) is larger than the filling port (1202). When the circular cover plate (130) is rotated to the filling port (1202), the circular cover plate (130) can completely cover the filling port (1202).
10. The experimental apparatus for inducing EMT in synovial sarcoma cells according to claim 9, characterized in that, When the circular cover plate (130) covers the filling port (1202), a locking structure is also installed between the circular cover plate (130) and the cylindrical cover (120); The locking structure includes a locking hole A (1203) opened at the outer edge of the filling port (1202) and a locking hole B (1302) opened at the edge of the circular cover plate (130) and corresponding to the position of the locking hole A (1203). The shape and size of the locking hole A (1203) are matched with the shape and size of the locking hole B (1302); When the locking hole B (1302) coincides with the locking hole A (1203), a locking pin (140) is inserted into the locking hole B (1302) and the locking hole A (1203). After the locking pin (140) is inserted, it locks and fixes the circular cover plate (130).