METHODS FOR PRODUCING BIOCONJUGATION PRODUCTS OF E. COLI O-ANTIGON POLYSACCHARIDES, COMPOSITIONS THEREOF, AND METHODS OF USING THEM

Abstract

Methods are provided for producing bioconjugates of O-antigen polysaccharides covalently linked to a carrier protein using recombinant host cells. The recombinant host cells used in the methods described herein encode a particular oligosaccharyl transferase enzyme depending on the O-antigen polysaccharide bioconjugate intended for production. The oligosaccharyl transferase enzymes can be Pg1B oligosaccharyl transferase or variants thereof. Also provided are compositions containing the bioconjugates, and methods of using the bioconjugates and compositions described herein for vaccinating an individual against extra-enteric pathogenic E. coli (ExPEC).