METHOD FOR ACTIVATING MITOCHONDRIA

Abstract

A device for activating the mitochondrion, wherein negative charge and reactive oxygen are delivered from outside to the mitochondrion via a negative charge and reactive oxygen delivery unit to serve as material for the mitochondrial redox reaction; and, wherein a calcium ion channel stimulation unit can stimulate an in vivo calcium ion to transport a protein across a calcium ion on an inner membrane of the mitochondrion into a matrix space of the mitochondrion, whereby an influx of the calcium ion promotes the depolarization of the mitochondrion and increases the activity of mitochondrial oxidative phosphorylation.

Description

BACKGROUND OF THE INVENTION The present invention relates to a device for activating mitochondria and, in particular, for supplying the mitochondrion with negative charges (-e) and reactive oxygen (O) ex vivo by means of a unit for supplying negative charges and reactive oxygen. In this process, calcium ions are stimulated in vivo to pass through the calcium on the mitochondrial endomembrane in order to transfer proteins into the mitochondrial matrix via a stimulation unit of a calcium ion channel, thereby promoting mitochondrial activation and the formation of adenosine triphosphate (ATP). DESCRIPTION OF THE STATE OF THE ART The mitochondrion is a unit responsible for energy production within the cell, essentially the cell's "powerhouse." Each mitochondrion consists of an outer membrane layer and an inner membrane layer, and the space enclosed by the inner membrane is called the matrix. The outer membrane of a mitochondrion is equipped with enzymes involved in biological oxidation, providing materials necessary for reactions within the mitochondrion. Within the mitochondrion, the matrix contains enzymes capable of metabolizing and converting carbohydrates, proteins, and fats into H₂O and CO₂ via the citric acid cycle. During this process, electrons are transferred through the electron transport chain and synthesized into adenosine triphosphate (ATP) through oxidative phosphorylation. ATP is the primary energy source for energy-consuming reactions in plants and animals.ATP is a type of nucleotide in biology that serves as an energy currency for intracellular energy transfer, storing and transmitting chemical energy. ATP plays a crucial role in nucleic acid synthesis. Furthermore, ATP is the disodium guanylate in RNA and acts as a substitute during DNA transcription. However, energy production in the mitochondria requires a continuous redox reaction. The activation power of the redox reaction depends on the negative charge (-e) and the reactive oxygen species (O), which are the materials the mitochondria need to function. When these materials can be supplied to the mitochondria, the mitochondria become more active. Furthermore, the influx of calcium ions plays a crucial role in mitochondrial activation and ATP production, as follows: 1. Control of calcium ions within the mitochondrion: Calcium ions can enter the mitochondrion and influence its function. When the calcium level in the cytoplasm rises, calcium ions transport proteins across the calcium on the inner mitochondrial membrane into the mitochondrial matrix. The influx of calcium ions can promote mitochondrial depolarization and increase the activation of mitochondrial oxidative phosphorylation. 2. Promotion of oxidative phosphorylation: The increase in calcium ions activates various enzyme systems in the mitochondria, such as the key enzyme in the tricarboxylic acid cycle (TCA). The activation of these enzymes allows the mitochondria to accelerate the oxidation process of the substrate (also called zymolyte or functional substance) and increase the efficiency of the electron transport chain, ultimately contributing to the formation of ATP. 3. Increased ATP Synthesis: When calcium levels in the mitochondria rise, the tricarboxylic acid cycle (TCA cycle) and oxidative phosphorylation are accelerated. Oxidative phosphorylation is a type of cellular metabolic process that takes place in the inner mitochondrial membrane of eukaryotes or in the cell membrane of prokaryotes. The enzymes and energy released during the oxidation of various nutrients are used for ATP synthesis, directly increasing ATP production. ATP production is driven by the electron transport chain, and the processes promoted by calcium ions ensure the continuity and efficiency of ATP production. 4. Maintaining the cell's energy balance: Increased ATP production contributes to maintaining the cell's energy balance, especially under various stresses. This is also beneficial for cell repair and the restoration of normal function, thus preventing cell death due to energy deficiency. Therefore, the activation of the mitochondria and ATP production are increased by the continuous supply of negative charges (-e) and reactive oxygen (O), which are required from the outside for the mitochondrial redox reaction, as well as the promotion of calcium ions in vivo, which transport proteins via the calcium on the inner membrane of the mitochondrion into the matrix space of the mitochondrion. SUMMARY OF THE INVENTION A primary objective of the present invention is to provide a method for activating the mitochondria that increases mitochondrial activation and ATP production. The main feature of the present invention relates to a supply of negative charges and reactive oxygen from the outside via the units for supplying negative charges and reactive oxygen to the mitochondrion to serve as material for the mitochondrial redox reaction, and a stimulation unit of the calcium ion channel that enables the in vivo calcium ions to transport proteins via the calcium on the inner membrane of the mitochondrion into the matrix space of the mitochondrion, so that the influx of calcium ions can promote the depolarization of the mitochondrion and increase the activation of the oxidative phosphorylation of the mitochondrion, thereby increasing the activation of the mitochondrion and ATP production. To better understand the technical means for achieving the above objective and the effect of the present invention, detailed descriptions are given below with regard to the following exemplary embodiment. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a block diagram of a preferred embodiment of the present invention. As state of the art, reference is made to the document “TAIWAN MITOCHONDRION APPLIED TECHNOLOGY Co., Ltd: YIN FU RUI DE” Far Infrared Ray Systemic Therapy Device (CE-1887-A) is used to test the Mitochondrion Activation of Peripheral Blood Mononuclear Cell. Test Service Report.

[2020] ". DETAILED DESCRIPTION OF THE IMPLEMENTATION EXAMPLES OF THE INVENTION With reference to Fig. 1, the device for activating the mitochondrion according to a preferred embodiment of the present invention is as follows: The unit for supplying negative charges and reactive oxygen species (ROS) delivers negative charges (-e) and reactive oxygen species (O) from outside the body to the mitochondria as material for mitochondrial redox reactions. This unit can be implemented using an electrified low-temperature plasma generator. After electrification, the low-temperature plasma generator produces a large quantity of negative charges (-e) and reactive oxygen species (O), which then enters the human body to serve as material for mitochondrial redox reactions. The stimulation unit of calcium ion channel 2 stimulates in vivo calcium ions to transport proteins across the calcium on the inner mitochondrial membrane into the mitochondrial matrix, thus promoting mitochondrial depolarization. The stimulation unit of calcium ion channel 2 can be a far-infrared generator with a power output exceeding 500W, and the body irradiated by this powerful far-infrared generator can stimulate in vivo calcium ions to transport proteins across the calcium on the inner mitochondrial membrane into the mitochondrial matrix. The unit for providing negative charges and reactive oxygen 1 in the above-mentioned step 1 is intended to adequately supply the mitochondrion with negative charges (-e) and reactive oxygen (O), and the stimulation unit of the calcium ion channel 2 in step 2 is intended to stimulate the in vivo calcium ions to transport the proteins across the calcium on the inner membrane of the mitochondrion into the matrix space of the mitochondrion, so that the influx of calcium ions can promote the depolarization of the mitochondrion and increase the activation of the mitochondrion as well as ATP production. Furthermore, Taiwan Mitochondrion Applied Technology Co., Ltd. tested the mitochondrial activation of peripheral blood mononuclear cells (PBMCs) in test subjects A, B, and C before using the present invention, one hour after the second use, and one hour after the fourth use. The test results are presented in the first, second, and third test reports in the prior art document cited. According to the test results, the mitochondria are significantly activated after application of the present invention. The test results demonstrate that the present invention is indeed effective and has value for industrial applications.

Claims

A device for activating the mitochondrion, wherein negative charge and reactive oxygen are delivered from outside to the mitochondrion via a negative charge and reactive oxygen delivery unit to serve as material for the mitochondrial redox reaction; and, wherein a calcium ion channel stimulation unit can stimulate an in vivo calcium ion to transport a protein across a calcium ion on an inner membrane of the mitochondrion into a matrix space of the mitochondrion, whereby an influx of the calcium ion promotes the depolarization of the mitochondrion and increases the activity of mitochondrial oxidative phosphorylation. Device for activating the mitochondrion according to claim 1, wherein the unit for providing negative charges and reactive oxygen is a low-temperature plasma generator. Device for activating the mitochondrion according to claim 1, wherein the stimulation unit of the calcium ion channel is a far-infrared generator with a power output exceeding 500W.

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