Labeling and markers for the analysis of a biological sample

Abstract

Label (100, 200, 500, 702, 802, 900, 901, 1000, 1200, 1300, 1600) for the analysis of a biological sample, comprising a nucleic acid scaffold (102, 502, 1002, 1102, 1202, 1301), a first plurality of labeling units (104), wherein the nucleic acid scaffold (102, 502, 1002, 1102, 1202, 1301) has a duplex structure comprising at least 50% of the nucleotides of the nucleic acid scaffold (102, 502, 1002, 1102, 1202, 1301), wherein the nucleic acid scaffold (102, 502, 1002, 1102, 1202, 1301) a major oligonucleotide (106, 504, 902, 910, 1004, 1104, 1302, 1602) comprising at least one first nucleotide sequence (506), wherein the nucleic acid framework (102, 502, 1002, 1102, 1202, 1301) comprises at least one second nucleotide sequence (508), and wherein at least the first nucleotide sequence (506) and the second nucleotide sequence (508) form the duplex structure of the nucleic acid framework (102, 502, 1002, 1102, 1202, 1301), wherein the nucleic acid framework (102, 502, 1002, 1102, 1202,1301) comprising at least one auxiliary oligonucleotide (108, 906, 912, 1006, 1106, 1204, 1304, 1604) comprising the second nucleotide sequence (508), wherein the labeling units (104) are bound to the nucleic acid scaffold (102, 502, 1002, 1102, 1202, 1301) at a distance from each other in a range of 8 to 20 nucleotides.