MOLECULAR DETECTION OF PARECHOVIRUS

DE602015093271T2Active Publication Date: 2026-04-15QUEST DIAGNOSTICS INVESTMENTS INC
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Patent Information

Authority / Receiving Office
DE · DE
Patent Type
Patents
Current Assignee / Owner
Filing Date
2015-04-28
Publication Date
2026-04-15

AI Technical Summary

Technical Problem

Existing methods for detecting and differentiating enterovirus and parechovirus in biological samples are complex, requiring multiple steps and the extraction of nucleic acids, which is time-consuming and inefficient for rapid diagnosis.

Method used

A method using specific primer pairs (SEQ ID NO:1-6) for amplifying enteroviral and parechoviral nucleic acids without prior extraction, involving heating, reverse transcription, and real-time PCR in a centrifugal microfluidic disc for rapid detection and differentiation.

Benefits of technology

Enables rapid, efficient detection and differentiation of 64 enterovirus serotypes and 8 parechovirus serotypes directly from unprocessed biological samples, reducing the need for nucleic acid extraction and simplifying the diagnostic process.

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