Liquid composition for inhalation for electronic cigarettes with reduced cytotoxicity

Abstract

The present invention relates to a liquid inhalation composition for electronic cigarettes with reduced cytotoxicity. In particular, the present invention relates to a composition for inhalation by means of electronic cigarettes comprising: - a glycerol / propylene glycol mixture in a percentage by volume between 80% and 99%; - optionally, water in a percentage by volume less than or equal to 6%, preferably less than or equal to 5%; 5%; - optionally, nicotine in a percentage by weight / volume not exceeding 0.2%; - optionally, flavorings in a percentage by volume less than or equal to 15%, preferably less than or equal to 10%; - a pH adjuster, wherein the composition has a pH between 7.0 and 7.6.

Description

Field of application of the invention

[0001] The present invention relates to a PH adjuster acid for use in preventing pulmonary cytotoxicity in subjects intaking vapors by means of electronic cigarettes.Background art

[0002] Recognizing that cigarette smoking causes serious damage to health has long prompted the industry to develop alternative forms of inhalation which can give smokers adequate satisfaction and effectively replace traditional cigarettes in daily use.

[0003] Starting from the evidence that the intake of nicotine is a key factor which causes the continuous desire to smoke in smokers, but that the greatest damage comes from the substances of the high-temperature combustion of tobacco, such as polycyclic aromatic hydrocarbons, tar and carbon monoxide, the art has followed two parallel paths. On the one hand, systems have been developed which include heating tobacco at a controlled temperature (generally no higher than 350°C), so as to avoid the harmful effects of high-temperature combustion. On the other hand, so-called "electronic cigarettes" have been created which vaporize a liquid comprising, in addition to a base capable of providing a sufficiently dense body to the vapor released, also a controlled amount of nicotine and various types of flavorings.

[0004] The heated-tobacco inhalation systems are those which are most similar to traditional smoking for smokers, both in terms of the flavoring of the tobacco and of consumption methods and times, similar to those of a cigarette. However, it should be remembered that some toxic substances contained in tobacco are formed even before the combustion thereof, such as, in addition to nicotine, nitrosamines. These substances are therefore not eliminated by reducing the combustion temperature to no more than 350°C.

[0005] The compositions for electronic cigarettes, on the other hand, do not contain such toxic or carcinogenic substances, apart from a controlled amount of nicotine, which is essential to make the inhalation of the composition pleasant by the traditional smoker. Furthermore, the possibility of flavoring the composition in various manners forms a further attraction.

[0006] Therefore, electronic cigarettes largely solve the toxicity problems associated with tobacco use, even if they are not completely safe. In fact, the base composition, which consists for the most part of a mixture of propylene glycol and glycerol in a volume ratio of 50:50, or for a denser vapor of 30:70, has an inherent toxicity, albeit much more reduced than that of a traditional cigarette and the heated-tobacco system described above.

[0007] CN 110 558 605 A discloses an e-cigarette liquid comprising a base solvent including propylene glycol and glycerol, nicotine, a pH adjuster, a cooling agent (e.g. menthol) and a flavor, with a pH between 7.0 and 7.5. This document however does not disclose the use of an acid pH adjuster in reducing the cytotoxicity of the composition.

[0008] Therefore, the problem of being able to further lower the toxicity of a composition for inhalation by means of electronic cigarettes so as to make the prolonged consumption thereof much safer is still felt.Summary of the invention

[0009] The aforesaid technical problem is substantially solved by a pH adjuster acid comprising the technical features set out in one or more of the appended claims, the definitions of which form an integral part of the present description for the purpose of sufficiency of description.

[0010] Therefore, the present invention relates to a pH adjuster acid for use in preventing pulmonary cytotoxicity in subjects intaking vapors by means of electronic cigarettes, wherein said pH adjuster acid is added to an electronic cigarette composition comprising a glycerol / propylene glycol mixture in a percentage by volume between 80% and 99%, optionally water in a percentage by volume less than or equal to 6%, preferably less than or equal to 5%, nicotine in a percentage by weight / volume not exceeding 0.2%, optionally flavorings in a percentage by volume less than or equal to 15%, preferably less than or equal to 10%, and wherein said pH adjuster acid gives said composition a pH between 7.2 and 7.6.

[0011] The present disclosure also describes a base composition (not part of the invention) comprising: a glycerol / propylene glycol mixture in a percentage by volume between 93% and 99%; optionally, water in a percentage by volume less than or equal to 6%, preferably less than or equal to 5%; nicotine in a percentage by weight / volume not exceeding 0.2%; a pH adjuster, in which the base composition has a pH between 7.2 and 7.6.

[0012] Further features and advantages of the present invention will become more apparent from the indicative and thus non-limiting description of a preferred, but not exclusive embodiment of the invention.Brief description of the drawings

[0013] Figure 1 depicts a graph of cell viability vs composition concentration with a control compound; Figure 2 depicts a graph of cell viability vs composition concentration with a composition including a pH adjuster according to the invention; Figure 3 depicts a graph of cell viability vs composition concentration with a composition for electronic cigarettes including a pH adjuster according to the prior art; Figure 4 depicts a graph of cell viability vs composition concentration with a heated-tobacco system according to the prior art. Detailed description of the invention

[0014] The present invention relates to the use of a pH adjuster acid in a composition for inhalation by means of electronic cigarettes comprising: a glycerol / propylene glycol mixture in a percentage by volume between 80% and 99%; optionally, water in a percentage by volume less than or equal to 6%, preferably less than or equal to 5%; nicotine in a percentage by weight / volume not exceeding 0.2%; optionally, flavorings in a percentage by volume less than or equal to 15%, preferably less than or equal to 10%; a pH adjuster, in which the composition has a pH between 7.2 and 7.6.

[0015] The glycerol / propylene glycol mixture is at a volume ratio between 50:50 and 70:30.

[0016] If the composition contains flavorings, the percentage by volume of the glycerol / propylene glycol mixture will not be greater than 90%.

[0017] The glycerol is preferably vegetable glycerol.

[0018] The percentage of nicotine in the composition should not be greater than the amount allowed by current legislation, in particular it should be less than or equal to 20 mg / ml.

[0019] The water is preferably demineralized water.

[0020] The flavorings can be of any type normally used for electronic cigarettes, such as fruit flavorings, tobacco flavorings, mentholates or mixtures thereof. The flavorings can be added to a base composition which does not contain them directly by the smoker. In this case, glycol-based flavorings are available to add to the composition as desired.

[0021] Therefore, the disclosure further describes a base composition comprising a glycerol / propylene glycol mixture in a percentage by volume between 93% and 99%; optionally, water in a percentage by volume less than or equal to 6%, preferably less than or equal to 5%; nicotine in a percentage by weight / volume not exceeding 0.2%; a pH adjuster, in which the base composition has a pH between 7.2 and 7.6.

[0022] The pH adjuster is preferably an organic or inorganic acid, more preferably selected from orthophosphoric acid, lactic acid and citric acid.

[0023] The weight percentage of the pH adjuster in the composition depends on both the nature of the pH adjuster and the percentage of the various components used in the composition. For example, in a composition containing about 90% by volume of 50:50 glycerol / propylene glycol mixture, about 5% water, about 10% flavorings (apple / kiwi), nicotine 8 mg / ml, orthophosphoric acid in a weight percentage of 0.1% adjusted the pH of the composition to 7.5.

[0024] The pH is between 7.2 and 7.6, preferably between 7.3 and 7.6 or between 7.4 and 7.6 or is of about 7.5.

[0025] The present invention originates from the evidence that the known compositions for electronic cigarettes have a basic pH. The present Applicant has conducted experiments and has verified that such a basic pH is responsible for a certain cytotoxicity towards lung cells (human lung fibroblasts). The Applicant has also discovered that by lowering the pH of the composition to a pH between 7 and 7.6, such toxicity is substantially reduced if not eliminated.Experimental section

[0026] The cytotoxicity of a composition according to the invention towards compositions of the prior art was evaluated through the MTT test: cell survival test with human fibroblasts of pulmonary origin grown in monolayer for the evaluation of the biocompatibility of smoke with mucous membranes.

[0027] The test aims at evaluating the cytotoxicity of the smoke condensate obtained with electronic cigarettes with pH correction to neutrality according to the invention on a cell culture of human lung fibroblasts, as described in the UNI EN ISO 10993-5 and ISO 4387:2019 standards related to the biological evaluation of medical devices, appropriately modified for the purpose.

[0028] The cytotoxicity of the condensate obtained by simulating the exposure to which the user of the electronic cigarette is subjected is an indicator of the safe use of the device in hand and the overall toxicity thereof towards the respiratory epithelium.

[0029] The method used is that of MTT, which is simple and reproducible, originally developed by Mossman.

[0030] An increase or decrease in viable cells results in a concomitant change in absorbance which can be regarded as an indicator of the degree of cytotoxicity caused by exposure to the test substances. The cells are also examined under a microscope after contact with the sample to evaluate any changes in the morphology thereof, membrane integrity or any lysis phenomena.

[0031] A line of Human Lung Fibroblasts (HPF) was used. Source: Innoprot. The cells are cultured in MEM containing 10% FBS and antibiotics.

[0032] Cigarette smoke is sampled using a smoke machine consisting of a vacuum system and a 100 ml flask connected to an electronic cigarette. The electronic cigarette is positioned vertically on an inert plastic fitting. The positioning allows the metal resistance inside the tank and used to heat the liquid to always remain in contact with the liquid itself, as indicated by the manufacturer.

[0033] The electronic cigarette loaded with the appropriate liquid was left to rest about 20 minutes before aspiration, so that the cotton inside the tank was correctly moistened with liquid, after which the first 2 / 3 puffs were discarded.

[0034] The flask is connected by silicone tubes to a flow meter inside the instrument, which is in turn connected to a vacuum pump and to a PC with control software. The presence of a flow meter allows the constant setting of the aspiration flow. 30 ml of sterile medium were used for the preparation of the condensates. An aspiration / inhalation simulation is performed with a duration of 3" with a pause lasting 45" between one aspiration and the other, to facilitate the collection of the condensate in the medium, keeping the flask constantly under stirring during the experiment, in accordance with ISO 4387:2019.

[0035] The aspiration flow is regulated at 1 L / min. 150 aspiration / inhalation cycles are performed, equal to 25 traditional cigarettes. The smoke from the electronic cigarette, loaded with the special liquid at the beginning of the session, is continuously sampled for the expected number of cycles. The medium with the condensate obtained from such an experiment was brought to pH 7.5 with lactic acid and subjected to cytotoxicity tests.

[0036] Human lung cells were seeded in 96-well plates and allowed to grow for 24h at 37°C and 5% CO 2 . On the second day, the medium was removed and replaced by the freshly prepared condensate from the cigarette to be tested.

[0037] In order to determine the IC 50 value (Inhibiting Concentration 50) at the end of the experiment, the tested concentrations are the condensate as is and the subsequent serial 1:2 dilutions thereof in medium.

[0038] Each sample was tested in sixfold. Untreated cells were used as blank and as positive control the cells were treated with a surfactant of known toxicity (Sodium Lauryl Sulfate - SLS) dissolved in the culture medium at concentrations between 0.5 mg / ml and 0.03 mg / ml.

[0039] After 24 hours of incubation with the sample and the control, the cytotoxicity test (MTT) was then performed to evaluate the cell survival percentage.MTT cell viability tests

[0040] The culture medium is aspirated and the cells incubated in 100 µl / well of a 1 mg / ml solution of MTT, for 2 hours at 37°C. The solution is removed and replaced by 200 µl / well of DMSO with subsequent 30' of incubation at room temperature and stirring at medium speed.

[0041] The absorbance at 570 nm is read with a colorimeter (Tecan INFINITE F200) provided with a plate reader by subtracting the background reading at 650 nm.

[0042] The result is expressed as a percentage of cell viability according to the formula:

[0043] In case it is possible to calculate the IC 50 value (Inhibiting Concentration 50) which indicates the concentration of the product which inhibits cell viability by 50%. Conc >50 = first concentration which gave a cell viability above 50% Conc <50 = first concentration which gave a cell viability less than 50% % >50 = first cell viability obtained above 50% % <50 = first cell viability obtained less than 50%.

[0044] The following acceptability criteria of the method were adopted: for the blank: the mean OD value of the replicates should be ≥ 0.2, the standard deviation should be ≤ 18%, the mean of two columns of blanks should not differ by more than 15%; for the positive control (CP): the IC 50 of the SLS positive control should be 0.10 mg / ml ± 0.03, the standard deviation should be ≤ 18%; for the sample: standard deviation should be ≤ 18%.

[0045] A reduction in cell viability > 30% is considered a cytotoxic effect.

[0046] The samples used were: composition for electronic cigarettes including a pH adjuster acid according to the invention, comprising 90% by volume of 50:50 glycerol / propylene glycol mixture, water about 5%, flavorings (apple / kiwi) about 10%, nicotine 8 mg / ml, pH adjuster: acid lactic up to pH 7.5; composition for electronic cigarettes of the prior art, comprising 90% by volume of 50:50 glycerol / propylene glycol mixture, water about 5%, flavorings (apple / kiwi) about 10%, nicotine 8 mg / ml, pH 8.9; HEETS Turquoise selection cigarette 3172 / 20-06 (heated-tobacco cigarette), pH 7.9.

[0047] Figure 1 shows the graph of cell viability vs concentration for the positive control (SLS).

[0048] Figure 2 shows the graph of cell viability vs concentration for the composition including a pH adjuster acid of the invention at pH 7.5.

[0049] Figure 3 shows the graph of cell viability vs concentration for the electronic cigarette composition of the prior art (pH 8.9).

[0050] Figure 4 shows the graph of cell viability vs concentration for the HEETS Turquoise selection heated-tobacco cigarette.

[0051] The following IC 50 values were calculated from the data of such experiments: positive control IC 50 = 0.09 ml / ml composition for electronic cigarettes including a pH adjuster acid according to the invention IC 50 »1 ml / ml (non-cytotoxic at the highest concentration tested) composition for electronic cigarettes of the prior art IC 50 = 0.93 ml / ml IC 50 heated-tobacco cigarette = 0.19 ml / ml.

[0052] From the above it is apparent that the composition for electronic cigarettes including a pH adjuster acid according to the invention is much less cytotoxic than both the composition of the prior art without acidity adjuster and, to an even greater extent, the heated-tobacco cigarette.

[0053] Therefore, the invention relates to a pH adjuster acid for use in preventing pulmonary cytotoxicity in subjects intaking vapors by means of electronic cigarettes, in which said pH adjuster acid is added to an electronic cigarette composition comprising a glycerol / propylene glycol mixture in a percentage by volume between 80% and 99%, optionally water in a percentage by volume less than or equal to 6%, preferably less than or equal to 5%, nicotine in a percentage by weight / volume not exceeding 0.2%, optionally flavorings in a percentage by volume less than or equal to 15%, preferably less than or equal to 10%, and in which said pH adjuster acid gives said composition a pH between 7.2 and 7.6.

[0054] Said pH adjuster acid is preferably selected from orthophosphoric acid, lactic acid and citric acid and the composition for electronic cigarettes is preferably as defined above.

Claims

1. A pH adjuster acid for use in preventing pulmonary cytotoxicity in subjects intaking vapors by means of electronic cigarettes, wherein said pH adjuster acid is added to an electronic cigarette composition comprising a glycerol / propylene glycol mixture in a percentage by volume between 80% and 99%, optionally water in a percentage by volume less than or equal to 6%, preferably less than or equal to 5%, nicotine in a percentage by weight / volume not exceeding 0.2%, optionally flavorings in a percentage by volume less than or equal to 15%, preferably less than or equal to 10%, and wherein said pH adjuster acid gives said composition a pH between 7.2 and 7.6.

2. The pH adjuster acid for the use according to claim 1, wherein the glycerol / propylene glycol mixture is at a volume ratio between 50:50 and 70:30.

3. The pH adjuster acid for the use according to claim 1 or 2, wherein, when the composition contains flavorings, the percentage by volume of the glycerol / propylene glycol mixture is no greater than 90%.

4. The pH adjuster acid for the use according to any one of claims 1 to 3, wherein the flavorings are selected from fruit, tobacco, mentholated flavorings or mixtures thereof.

5. The pH adjuster acid for the use according to any one of claims 1 to 4, wherein the pH adjuster is an organic or inorganic acid.

6. The pH adjuster acid for the use according to claim 5, wherein the pH adjuster is selected from orthophosphoric acid, lactic acid and citric acid.

7. The pH adjuster acid for the use according to any one of claims 1 to 6, wherein the pH is between 7.3 and 7.6, or between 7.4 and 7.6, or is of about 7.5.

8. The pH adjuster acid for the use according to any one of claims 1 to 7, wherein the pH adjuster is orthophosphoric acid in a percentage by weight of about 0.1%.

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