Use of charged air ions in cosmetic treatments

Abstract

Disclosed herein are methods for treatment of skin conditions and methods of cosmetic treatment utilising non-contact eletrostimulation (ES), which is used as a means for effecting rejuvenation of skin as well as general and specific improvements in skin appearance. In preferred embodiments the ES is effected by application of ionized gas ions. A further embodiment relates to combination of non-contact ES and topical administration of cosmetic or pharmaceutical substances, the effects of which are augmented by the non-contact ES.

Description

[0001] USE OF CHARGED AIR IONS IN COSMETIC TREATMENTS

[0002] FIELD OF THE INVENTION

[0003] The present invention relates to the field of cosmetic treatment. In particular, the present invention relates to the use of non-contact eletrostimulation (ES) as a means for effecting rejuvenation of skin as well as general and specific improvements in skin appearance. The invention also relates to to combination of non-contact ES and topical administration of cosmetic or pharmaceutical substances.

[0004] BACKGROUND OF THE INVENTION

[0005] Electrical stimulation (also termed electrostimulation or ES) is a well-known technology, which has been used in treatment of a large number of pathologies - when used for medical treatment, ES is often termed electrotherapy.

[0006] Traditionally ES involves the transfer of electric current through an applied surface electrode pad that is in electrolytic contact with the external skin surface and the area to receive the effect. In ES, at least two electrodes (a lead and a discharge electrode) are required to complete the electric circuit.

[0007] ES has been used as a therapeutic means in a number of conditions, notably in the treatment of various types of wounds, such as acute wounds, burns, chronic wounds including pressure ulcers, diabetic ulcers, venous ulcers, and arterial ulcers.

[0008] A more recent development detailed in WO 2009 / 135896 substitutes the use of applied electrodes with application of air-borne ions from a device not in contact with the patient. Typically, the current / charge is delivered to the treated anatomic region via negatively charged O2ions, using e.g. an apparatus as the one disclosed in WO 2007 / 042029. This approach provides for a higher patient compliance, both because the application of air ions is non-painful and because the risk of infection in wounds is diminished. Later developments disclosed in WO 2015 / 052170 combines the gas ion application with light therapy.

[0009] In the field of cosmetic treatment, the traditional approaches for rejuvenation rely on the use of cosmetic compositions that seek to maintain or improve skin homeostasis via their content of i.a. nourishing, moisturizing, and keratinolytic compounds and substances. A different set of approaches utilise invasive (even surgical) procedures for applying fillers or for tightening skin, in particular in the facial area.

[0010] OBJECT OF THE INVENTION

[0011] It is an object of embodiments of the invention to provide a non-invasive, and non- pharmacological alternative to existing cosmetic treatments. It is a further object to provide a non-invasive approach for improving delivery of cosmetically active compounds to the skin.

[0012] SUMMARY OF THE INVENTION

[0013] It has been found by the present inventor(s) that application of a gas ion-induced microcurrent can deliver an electrical current to muscles and skin cells. This microcurrent builds up muscles in the face and / or lifts and tightens the skin tissue. In addition, the establishment of a microcurrent also facilitates delivery of active compounds commonly used for beauty treatments into the lower layers of the skin, whereby their beneficial effects are enhanced.

[0014] So, in a first aspect the present invention relates to a method for cosmetic treatment of a surface area of the skin of a human subject, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current.

[0015] In a second aspect, the present invention relates to ionized gas molecules for use in a method of treatment of an area of the skin of a human, the method being selected from redness relief, sensitivity relief, acne reduction, sun damage and / or photo damage prevention or repair, anti-inflammatory treatment of skin, treatment to ameliorate effects of laser treatment, treatment to ameliorate effects of cryolipolysis, and reduction in scar and pigmentation post-surgery, wherein the method comprises that ionized gas molecules are projected onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current. In a third aspect, the present invention relates to a method for therapeutic treatment of a surface area of the skin of a human subject by topic application on the skin of an active drug substance or composition comprising said substance, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current, and wherein the projection of the ionized gas molecules enhances the uptake and / or the therapeutic effect of the administered active drug substance or composition. A part of the third aspect relates to the same basic principle but in a non-therapeutic setting, and thus provides a method for non-therapeutic cosmetic treatment of a surface area of the skin of a human subject by topic application on the skin of a cosmetically active substance or composition comprising said active substance, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current, and wherein the projection of the ionized gas molecules enhances the uptake and / or the cosmetic effect of the administered active cosmetic substance or composition.

[0016] DETAILED DISCLOSURE OF THE INVENTION

[0017] Definitions

[0018] "Projection of ionized gas molecules" means that a sufficient amount of electrical charge in the form of electrically charged gas molecules is provided a surface area on the skin, so as to allow an electric current to be established between the surface area to surrounding tissue. It should be emphasized that the electric current, which passes from the surface area to the surrounding tissue cannot be established precisely - when discussing this electrical current in quantitative terms in the present application, the quantity (measured in Ampere or micro Ampere) is the one which may be measured in discharge electrode(s), which return(s) current from the treated human to the device that delivers the ionized gas molecules.

[0019] "Ionized gas molecules" broadly refers to gas molecules which carry a net electrical charge. Typical examples are negatively charged oxygen molecules and positively charged Nitrogen molecules because of the abundancy of oxygen and nitrogen in atmospheric air, but any gas molecule capable of carrying a net charge is useful in the practice of the present invention, as long as such ionized ga molecules do not provide unacceptable adverse or toxic effects on the human subjects treated.

[0020] A "discharge electrode" is an electrode attached to a treated subject which ensures that the ionized gas molecules do not simply build up a static charge in the subject but instead establishes an electrical current which passes through the subject to the discharge electrode. Typically, such an electrode will be in the form of a strap, but any convenient physical form may be useful. A special example of a discharge electrode is a "return electrode", which is connected (optionally via a control unit) to the device which supplies the ionized gas ions - this enables that a return current may be measured (cf. above) and this in turn enables full control over the current sent through the treated subject as a consequence of the delivery of the ionized gas molecules.

[0021] A "gas ion transmitting device" is a device or apparatus, which is capable of generating a stream, jet or flux of ionized gas molecules having a net electrical positive or negative charge and where the direction of the stream, jet or flux may optionally may be controlled with respect to dosage, intensity and direction. Specifics concerning such devices are discussed in the patent application publications referenced in the Background of the Invention section above.

[0022] Specific embodiments of the invention

[0023] In general, the projection of gas ions (and optionally light) onto the skin surface area can be carried out as set forth in WO 2009 / 135896 and WO 2015 / 052170, where the treatments are directed to various pathological states. However, details concerning timing of treatments, dosage of gas ions (in terms of the pA applied), shifting of current direction and ionized gas molecule charges disclosed in these patent publications apply mutatis mutandis to the present invention.

[0024] First aspect of the invention and embodiments thereof

[0025] The first aspect of the invention relates to method for cosmetic treatment of a surface area of the skin of a human subject, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current. The charge of the ionized gas molecules can be both negative and positive, depending on the selection of apparatus for air ion generation. However, in preferred embodiments the ionized gas molecules predominantly are negatively charged, such as negatively charged oxygen molecules (O2) (but as mentioned the ionized gas molecules can instead predominantly be positively charged, such as positively charged Nitrogen molecules (N2+)). At any rated, in most practical embodiments of the invention, the ionized gas molecules are derived from atmospheric air.

[0026] The projection of the ionized gas molecules preferably that an electrical current is established between the surface area of the skin and underlying tissue.

[0027] When treating a subject, the electrical current is typically at least 0.1 pA and at most 100 pA. However, any amperage in this interval can be used, e.g. about 0.2 pA, about 0.3 pA, about 0.4 pA, about 0.5 pA, about 0.6 pA, about 0.7 pA, about 0.8 pA, about 0.9, about 1 pA pA 2 pA, about 3 pA, about 4 pA, about 5 pA, about 6 pA, about 7 pA, about 8 pA, about 9 pA, about 10 pA, about 11 pA, about 12 pA, about 13 pA, about 14 pA, about 15 pA, about 16 pA, about 17 pA, about 18 pA, about 19 pA, about 20 pA, about 21 pA, about 22 pA, about 23 pA, about 24 pA, about 25 pA, about 26 pA, about 27 pA, about 28 pA, about 29 pA, about 30 pA, about 31 pA, about 32 pA, about 33 pA, about 34 pA, about 35 pA, about 36 pA, about 37 pA, about 38 pA, about 39 pA, about 40 pA, about 41 pA, about 42 pA, about 43 pA, about 44 pA, about 45 pA, about 46 pA, about 47 pA, about 48 pA, about 49 pA, about 50 pA, about 51 pA, about 52 pA, about 53 pA, about 54 pA, about 55 pA, about 56 pA, about 57 pA, about 58 pA, about 59 pA, about 60 pA, about 61 pA, about 62 pA, about 63 pA, about 64 pA, about 65 pA, about 66 pA, about 67 pA, about 68 pA, about 69 pA, about 70 pA, about 71 pA, about 72 pA, about 73 pA, about 74 pA, about 75 pA, about 76 pA, about 77 pA, about 78 pA, about 79 pA, about 80 pA, about 81 pA, about 82 pA, about 83 pA, about 84 pA, about 85 pA, about 86 pA, about 87 pA, about 88 pA, about 89 pA, about 90 pA, about 91 pA, about 92 pA, about 93 pA, about 94 pA, about 95 pA, about 96 pA, about 97 pA, about 98 pA, and about 99 pA.

[0028] Furthermore, irrespective of the type of ionized gas and the amperage, the treatment time is preferably at least 1 second and at most 100 minutes, but any treatment time in this interval is considered useful in the exercise of the invention. The treatment time can hence be about 2 s, about 3 s, about 4 s, about 5 s, about 6 s, about 7 s, about 8 s, about 9 s, about 10 s, about 11 s, about 12 s, about 13 s, about 14 s, about 15 s, about 16 s, about 17 s, about 18 s, about 19 s, about 20 s, about 21 s, about 22 s, about 23 s, about 24 s, about 25 s, about 26 s, about 27 s, about 28 s, about 29 s, about 30 s, about 31 s, about 32 s, about 33 s, about 34 s, about 35 s, about 36 s, about 37 s, about 38 s, about 39 s, about 40 s, about 41 s, about 42 s, about 43 s, about 44 s, about 45 s, about 46 s, about 47 s, about 48 s, about 49 s, about 50 s, about 51 s, about 52 s, about 53 s, about 54 s, about 55 s, about 56 s, about 57 s, about 58 s, about 59 s, about 1 minute, about 2 minutes, about 3 minutes, about 4 minutes, about 5 minutes, about 6 minutes, about 7 minutes, about 8 minutes, about 9 minutes, about 10 minutes, about 11 minutes, about 12 minutes, about 13 minutes, about 14 minutes, about 15 minutes, about 16 minutes, about 17 minutes, about 18 minutes, about 19 minutes, about 20 minutes, about 21 minutes, about 22 minutes, about 23 minutes, about 24 minutes, about 25 minutes, about 26 minutes, about 27 minutes, about 28 minutes, about 29 minutes, about 30 minutes, about 31 minutes, about 32 minutes, about 33 minutes, about 34 minutes, about 35 minutes, about 36 minutes, about 37 minutes, about 38 minutes, about 39 minutes, about 40 minutes, about 41 minutes, about 42 minutes, about 43 minutes, about 44 minutes, about 45 minutes, about 46 minutes, about 47 minutes, about 48 minutes, about 49 minutes, about 50 minutes, about 51 minutes, about 52 minutes, about 53 minutes, about 54 minutes, about 55 minutes, about 56 minutes, about 57 minutes, about 58 minutes, about 59 minutes, about 60 minutes, about 61 minutes, about 62 minutes, about 63 minutes, about 64 minutes, about 65 minutes, about 66 minutes, about 67 minutes, about 68 minutes, about 69 minutes, about 70 minutes, about 71 minutes, about 72 minutes, about 73 minutes, about 74 minutes, about 75 minutes, about 76 minutes, about 77 minutes, about 78 minutes, about 79 minutes, about 80 minutes, about 81 minutes, about 82 minutes, about 83 minutes, about 84 minutes, about 85 minutes, about 86 minutes, about 87 minutes, about 88 minutes, about 89 minutes, about 90 minutes, about 91 minutes, about 92 minutes, about 93 minutes, about 94 minutes, about 95 minutes, about 96 minutes, about 97 minutes, about 98 minutes, and about 99 minutes.

[0029] In preferred embodiments, the treatment detailed above is carried out in combination with light treatment - for details on useful light treatment modes, reference is e.g. given to WO 2015 / 052170.

[0030] Embodiments of the invention further entail that method of the first aspect comprises that the ionized gas molecules are projected by a gas ion transmitting device, which optionally can further be equipped with a camera and / or a light emitting means.

[0031] The cosmetic treatment is in embodiments of the invention selected from the group consisting of wrinkle depth reduction, skin tightening, skin rejuvenation, skin elasticity enhancement, skin hydration enhancement, collagen stimulation, skin peeling, scar revision, dermabrasion, anti-aging, skin discoloration reduction, fine line improvement, redness relief, sensitivity relief, exfoliation, acne reduction, youth glow induction, sun damage and / or photo damage prevention or repair, anti-inflammatory treatment of skin, treatment to ameliorate effects of laser treatment, treatment to ameliorate effects of cryolipolysis, and reduction in scar and pigmentation post-surgery.

[0032] In further preferred embodiments, the cosmetic treatment is selected from the group consisting of wrinkle depth reduction, skin tightening, skin rejuvenation, skin elasticity enhancement, skin hydration enhancement, collagen stimulation, skin peeling, scar revision, dermabrasion, anti-aging, skin discoloration reduction, fine line improvement, exfoliation, and youth glow induction.

[0033] In certain embodiments, the cosmetic treatment method is carried out in combination with topical administration of at least one cosmetically active substance or composition. As supported by the findings reported in the example, the delivery of the at least one cosmetically active substance or composition is preferably enhanced by the projection of the ionized gas molecules and / or the activity of the at least one cosmetically active substance is enhanced by the projection of the ionized gas molecules. Examples of cosmetically active substances are for instance vitamins and vitamin precursors, metal salts, moisturizing agents, lipids, fats or fatty acids, e.g. in liposomal form, peptides or polypeptides, amino acids.

[0034] In these cases, the at least on cosmetically active substance is preferably selected from the group consisting of beta carotene and / or other carotenoids, selenium or a selenium salt, manganese or a manganese salt, one or more anti-oxidants, glutathione, coenzyme Q10, lipoic acid, one or more flavonoids, one or more phenolic compounds (such as phenolic compounds selected from Quercetin, one more catechins, resveratrol, coumaric acid, one or more anthocyanins, one or more polyphenols, and one or more phytoestrogens), one or more vitamins (e.g. selected from the group consisting of vitamin A, retinol, B3, vitamin C, vitamin E, and vitamin D), iron or an iron salt, magnesium or a magnesium salt, one or more peptides (including synthetic peptides, such as Matrixyl 3000®), one or more proteins, one or more amino acids, one or more essential oils or fats, platelet-rich plasma (PRP), collagen, snail mucin, and hyaluronic acid.

[0035] Second aspect of the invention and embodiments thereof

[0036] This aspect relates to ionized gas molecules for use in a method of therapeutic treatment of an area of the skin of a human, the method being selected from redness relief, sensitivity relief, acne reduction, sun damage and / or photo damage prevention or repair, antiinflammatory treatment of skin, treatment to ameliorate effects of laser treatment, treatment to ameliorate effects of cryolipolysis, and reduction in scar and pigmentation post-surgery, wherein the method comprises that ionized gas molecules are projected onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current. The method in question hence relates to a subset of the treatments detailed in the 1staspect of the invention as well as the embodiments thereof discussed above. This also means that the features discussed above for the first aspect applies mutatis mutandis to the second aspect, i.e. that the method can be carried out as described above in the discussion of the method of the first aspect and the embodiments thereof.

[0037] Third aspect of the invention and embodiments thereof

[0038] This aspect relates to a) a method for therapeutic treatment of a surface area of the skin of a human subject by topic application on the skin of an active drug substance or composition comprising said substance, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current, and wherein the projection of the ionized gas molecules enhances the uptake and / or the therapeutic effect of the administered active drug substance or composition, or b) a method for non-therapeutic cosmetic treatment of a surface area of the skin of a human subject by topic application on the skin of a cosmetically active substance or composition comprising said active substance, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current, and wherein the projection of the ionized gas molecules enhances the uptake and / or the cosmetic effect of the administered active cosmetic substance or composition.

[0039] Thus both methods take advantage of the surprising finding in the example, namely that the projection of the ionized gas can enhance the effects of a topically applied substance, be it a drug substance or a substance applied for cosmetic purposes. In this context, the cosmetic substance can be any one of the cosmetic substances discussed above in the context of the first aspect of the invention.

[0040] The active drug substance is in the relevant cases selected from the group consisting of an antibiotic substance, an anti-inflammatory substance, a cytostatic or cytotoxic anti-cancer substance, a cytokine, a chemokine, a hormone, a receptor stimulant or activator, a receptor antagonist, a vasoactive substance, an analgesic, and an anaesthetic substance.

[0041] The exact formulation of the active drug substance or composition or the cosmetically active substance or composition can vary. For instance, a composition (therapeutic or cosmetic) will typically be selected from the group consisting of a suspension, an o / w emulsion, a w / o emulsion, and a suspoemulsion. Also, the active principle or the composition can be in the form of or is incorporated in a delivery system selected from a cream, a foam, a gel, a lotion, an ointment, a paste, a powder, a shake lotion, a solid, a sponge, a tape, a tincture, a solution, and a transdermal patch.

[0042] EXAMPLE 1

[0043] Human in vivo study

[0044] Protocol

[0045] A 6-week, study was performed on 10 women, aged 32-45 years. The whole face was initially treated with a cleansing serum (In this case a "Deep Cleanser" from FUSION MESO) for 5 minutes, followed by a massage, all over the face, with a serum cocktail, containing octapeptides, pentapeptides and low-molecular weight hyaluronic acid (in this case FUSION F-BTX).

[0046] Microcurrent was applied with a Wetling W200 device (available i.a. from Wetling EU ApS in Denmark) for 10 min, only on the left side of the face. The Device was adjusted to emit 1.5 pA without any light.

[0047] This procedure including treatment with cleansing serum, serum cocktail, and microcurrent was repeated three more times, for a total period of 40 minutes. At the end of the 40 min treatment period, the face was carefully cleaned, using a typical cleansing cream (e.g. FUSION FACE CREAM). The same therapy was repeated every day from Monday to Friday for 4 weeks. Changes on facial wrinkling were assessed by both expert grading and image analysis of digital photos (using an ARAM HUVIS DEVICE and a SKIN ANALYZER).

[0048] Results The combination of the microcurrent and the serum treatment significantly improved wrinkle appearance after the 6 weeks treatment, when compared to "only serum" regimen, according to the expert's conclusion. Skin analysis identified a 15% wrinkle reduction on the side of the face treated with ionized gas and serum.

[0049] EXAMPLE 2 Treatment protocols and treatment outcome

[0050] The following table lists various preferred treatment protocols according to the present invention.

[0051] The above indicated treatment regimens are evaluated according to their effects, which are summarized as follows:

[0052] Wrinkle depth reduction

[0053] Electrostimulation, as described in Rozhanets AR et al. 2010, Vopr Kurortol Fizioter Lech Fiz Kult. Jul-Aug;(4) :26-30. Russian. PMID: 21086592 and Paj^k et a / . Int J Environ Res Public Health. 2022 Jul 25; 19(15) :9055. doi: 10.3390 / ijerphl9159055. PMID: 35897441, has shown promising effects in reducing wrinkle depth and rejuvenating aging skin. In the study by Rozhanets et al., the combined application of electric myo-stimulation and meso-therapy demonstrated significant improvements in age-related facial changes. Electric myostimulation likely enhances muscle tone and circulation, contributing to a more youthful appearance. Meanwhile, Paj^k et a / . highlight the role of micro-needling in neck and cleavage rejuvenation, suggesting that it stimulates collagen production and enhances the penetration of skincare products. When coupled with electrostimulation according to the present invention, these techniques are by the present inventors believed to target different aspects of skin aging, leading to comprehensive wrinkle reduction and skin rejuvenation.

[0054] Skin tightening Electrostimulation, as explored in the studies by Chen et al. Biochem Pharmacol. 2023 Dec 9: 115979. doi: 10.1016 / j.bcp.2023.115979. Epub ahead of print. PMID: 38081367, Donadio et a!., Respir Med. 2022 May; 196: 106798. doi: 10.1016 / j.rmed.2022.106798. Epub 2022 Mar 1. PMID: 35306386, and Gerasimenko et al. Stomatologiia (Mosk). 2001;80(6) :52-5. Russian. PMID: 11881465, holds promise in promoting skin tightening through various mechanisms. Chen et al. investigated the cooperation of methotrexate and electrostimulation in alleviating psoriasiform skin inflammation, suggesting that electrostimulation may contribute to reducing skin laxity by suppressing inflammatory responses and possibly enhancing collagen production. In a different context, Donadio et al. examined the effectiveness of electrostimulation in improving muscle strength, but the principle may extend to skin tightening as well, as enhanced muscle tone can indirectly contribute to a firmer appearance. Additionally, Gerasimenko et al. discussed the potential of electrostimulation in the context of postoperative care after cheilorhinoplasty, indicating its role in promoting tissue healing and possibly aiding in skin tightening. These findings collectively support that electrostimulation according to the present invention could be a valuable adjunctive therapy for enhancing skin tightness and overall rejuvenation.

[0055] Skin rejuvenation

[0056] The research conducted by Xu et al. (Acta Meeh Sin. 2021;37(12) : 1843-1871. doi: 10.1007 / S10409-020-01026-2. Epub 2021 Feb 6. PMID: 33584001; PMCID: PMC7866966) and Chen & Yu (Dermatol Ther. 2003; 16(2) :87-92. doi: 10.1046 / j.1529-8019.2003.01619.x. PMID: 12919109) illuminates the potential of electrostimulation in skin rejuvenation. Xu et al.'s investigation likely offers valuable insights into how electrical stimulation affects the skin surface, potentially triggering processes such as increased blood circulation, nerve stimulation, and collagen synthesis, all of which are crucial for skin rejuvenation. Moreover, Chen 8<. Yu's exploration of acupuncture, electrostimulation, and reflex therapy in dermatology suggests that electrostimulation may modulate various skin functions, promoting cellular turnover and enhancing the skin's overall vitality and appearance. Together, these findings support the role of electrostimulation according to the present invention as a non-invasive method for skin rejuvenation, highlighting its potential to revitalize and refresh the skin.

[0057] Skin elasticity enhancement

[0058] The research conducted by Xu et al. (Acta Meeh Sin. 2021;37(12) : 1843-1871. doi:

[0059] 10.1007 / S10409-020-01026-2. Epub 2021 Feb 6. PMID: 33584001; PMCID: PMC7866966) and Chen & Yu (Dermatol Ther. 2003; 16(2) :87-92. doi: 10.1046 / j.1529-8019.2003.01619.x. PMID: 12919109) and Tangerino Filho et al. (Tissue Cell. 2016 Jun;48(3) :224-34. doi: 10.1016 / j.tice.2016.03.004. Epub 2016 Mar 9. PMID: 27138327) offers valuable insights into the potential of electrostimulation in enhancing skin elasticity. Xu et al.'s study provides understanding on how electrical stimulation influences the skin surface, potentially promoting collagen synthesis, improving blood circulation, and stimulating nerve activity, all of which contribute to enhanced skin elasticity. Additionally, Tangerino Filho et al.'s investigation into microcurrent therapy suggests that electrical stimulation can have positive effects on tissue repair and regeneration, which may extend to enhancing the elasticity of the skin. Furthermore, Gerasimenko et al.'s (supra) discussion of electrostimulation after cheilorhinoplasty implies its potential role in promoting tissue healing and possibly aiding in skin elasticity enhancement. These findings collectively support that electrostimulation holds promise as a non-invasive method for improving skin elasticity, offering potential benefits for skin health and appearance.

[0060] Skin hydration enhancement

[0061] The research by Tangerino Filho et al. (2016) also sheds light on the potential of electrostimulation, specifically microcurrent therapy, in enhancing skin hydration. Although the study primarily focuses on excisional elastic cartilage defects in young rats, the principles of microcurrent therapy are believed to extend to skin hydration enhancement. Microcurrent therapy may stimulate cellular activity, promoting the production of skin-supporting molecules such as hyaluronic acid and improving the skin's ability to retain moisture. Additionally, by facilitating tissue repair and regeneration, microcurrent therapy could indirectly contribute to enhanced skin hydration by restoring the skin's barrier function and optimizing its moisture balance. Overall, these findings therefore support that electrostimulation, particularly in the form of microcurrent therapy according to the present invention, holds promise as a non-invasive approach to improving skin hydration, offering potential benefits for skin health and overall appearance.

[0062] Anti-aqinq

[0063] The studies by Kwon 8i Moon (Ann Phys Rehabil Med. 2020 Nov;63(6) :474-482. doi: 10.1016 / j. rehab.2019.09.010. Epub 2019 Oct 24. PMID: 31669161) and Zacarias et al. (Int J Exp Pathol. 2023 Apr;104(2) :81-95. doi: 10. Ill 1 / iep.12469. Epub 2023 Feb 8. PMID: 36752313; PMCID: PMC10009304) provide insights into the potential of electrostimulation in the anti-aging process. Kwon 8i Moon's investigation into the regenerative effects of polydeoxyribonucleotide and microcurrent on rotator cuff healing suggests that electrostimulation may promote tissue repair and regeneration, which are essential aspects of anti-aging interventions. By stimulating cellular activity and enhancing collagen synthesis, electrostimulation is believed to contribute to the restoration of youthful skin characteristics, such as elasticity and firmness. Furthermore, Zacarias et al.'s research on the association of Arnica montana L. with microcurrent in accelerating dermis reorganization implies that electrostimulation may facilitate the repair of skin lesions and the rejuvenation of aging skin. Collectively, these findings support that electrostimulation according to the present invention holds promise as a non-invasive approach to combating signs of aging, offering potential benefits for skin health and overall anti-aging interventions.

[0064] Skin discoloration reduction and fine line improvement While a study by Jian et al. (Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2003 Sep;20(3) :494- 6. Chinese. PMID: 14565022) primarily investigates the effects of electrostimulation on soldiers' circadian rhythms of melatonin in plasma, it has been realized by the present inventors that the results indirectly suggest a potential role for electrostimulation in reducing skin discoloration. Melatonin is known to possess antioxidative properties and plays a role in regulating pigmentation processes in the skin. Therefore, by modulating melatonin levels or circadian rhythms through electrostimulation, it is plausible that this technique will indirectly influence skin pigmentation, potentially aid in reducing skin discoloration, and influence fine line improvement.

[0065] Redness relief

[0066] The research conducted by Lee et a / . (Int J Mol Sci. 2022 Feb 24;23(5) :2508. doi: 10.3390 / ijms23052508. PMID: 35269651; PMCID: PMC8910224) demonstrates the potential of micro-current stimulation, a form of electrostimulation, in providing relief from redness associated with inflammatory skin conditions. By investigating its effects on inflammatory responses in macrophages treated with peptidoglycan and in skin inflammation induced by Propionibacterium acnes, the study reveals that micro-current stimulation suppresses inflammatory pathways mediated by Toll-like receptor 2 (TLR2) and nuclear factor kappa B (NF-KB) . These pathways are known to play a crucial role in triggering and perpetuating inflammatory responses, including redness and irritation. Therefore, by inhibiting TLR2 / NF-KB signalling, micro-current stimulation has the potential to alleviate redness and soothe inflamed skin. This research hence support the promising role of electrostimulation according to present invention in providing relief from redness associated with inflammatory skin conditions, offering a non-invasive approach for managing skin sensitivity and irritation.

[0067] Sensitivity relief

[0068] Zacarias et al. (Int J Exp Pathol. 2023 Apr;104(2) :81-95. doi: 10.1111 / iep.12469. Epub 2023 Feb 8. PMID: 36752313; PMCID: PMC10009304) explored the potential of electrostimulation, specifically microcurrent therapy, in promoting exfoliation and dermal reorganization. By examining the effects of Arnica montana L. associated with microcurrent on skin lesions, the study suggests that electrostimulation accelerates the process of dermis reorganization, which may involve shedding of damaged or dead skin cells through exfoliation. Microcurrent therapy is known to stimulate cellular activity and promote tissue regeneration, which could facilitate the removal of dead skin cells and encourage the turnover of skin layers. Additionally, the association of Arnica montana L. with microcurrent may further enhance these effects, potentially intensifying the exfoliation process. Overall, these findings support that electrostimulation, particularly in the form of microcurrent therapy according to the present invention, holds promise as a non-invasive approach to promoting exfoliation and improving skin texture, offering potential benefits for skin health and rejuvenation.

[0069] Acne reduction

[0070] The authors of Lee H et al. (Int J Mol Sci. 2022 Feb 24;23(5) :2508. doi: 10.3390 / ijms23052508. PMID: 35269651; PMCID: PMC8910224) and Lazarenko NN et al. (Vopr Kurortol Fizioter Lech Fiz Kult. 2015 Jul-Aug;92(4) :41-47. Russian, doi: 10.17116 / kurort2015441-47. PMID: 26595968) both provide scientific substantiation for the application of electrical stimulation with bipolar pulsed currents for the combined treatment of patients presenting with acne vulgaris. Experiments on rabbits demonstrated the influence of bipolar pulsed currents on the cellular composition of lymph nodes and thereby facilitated the better understanding of certain theoretical aspects of the application of electrical stimulation at large. The clinical study on the application of multi-channel electrical stimulation and microcurrent therapy showed that these physical factors may cause remodeling of immunogenesis in the patents presenting with acne vulgaris manifested as the normalization of the cellular composition of peripheral blood (leukocyte, lymphocyte, T- and B-lymphocyte counts and immunoglobulin levels). These findings confirm the effectiveness of the proposed integrated treatment responsible for the enhancement of the overall resistance of the patents with acne vulgaris. Moreover, this therapeutic modality proved to exert the immunocorrective action and promote the restoration of the adaptive capacity at large. As a result, 80.9% of the patients presenting with acne vulgaris enjoyed prolonged remission of the disease. Moreover, the paper by Chen 8i Yu (Dermatol Ther. 2003; 16(2) :87-92. doi: 10.1046 / j.1529-8019.2003.01619.x. PMID: 12919109) delves into the potential of electrostimulation as a therapy for acne within the broader context of dermatology. Electrostimulation, along with acupuncture and reflex therapy, is explored for its effectiveness in addressing various dermatological conditions, including acne. While the specific details regarding the effects of electrostimulation on acne therapy are not outlined in depth in the provided citation, it can be inferred that electrostimulation may modulate skin functions and inflammatory processes relevant to acne pathogenesis. Through its potential to regulate sebum production, reduce inflammation, and promote tissue repair, electrostimulation could contribute to mitigating acne symptoms and promoting clearer skin, thus providing a sound basis for the application of the presently disclosed electrostimulation in acne treatment. Sun damage and / or photo damage prevention or repair

[0071] While the paper by Tsolakidis et al. (Burns. 2022 Aug;48(5) : 1230-1235. doi:

[0072] 10.1016 / j. burns.2021.09.021. Epub 2021 Sep 25. PMID: 34607727) primarily investigates the effects of wireless microcurrent stimulation on improving blood flow in burn wounds, the present inventors have realized that it indirectly suggests a potential role for electrostimulation in addressing sun damage or photo damage. Photo damage from prolonged sun exposure often results in compromised blood flow and impaired tissue healing. Therefore, interventions that improve blood flow, such as microcurrent stimulation, are believed to assist in the repair and regeneration of sun-damaged skin. By enhancing circulation, microcurrent stimulation may facilitate the delivery of oxygen and nutrients to the damaged tissue, promoting tissue repair and regeneration. Additionally, electrostimulation according to the present invention is believed to modulate cellular activities involved in the repair process, potentially accelerating the removal of damaged cells and promoting the synthesis of new collagen, which is essential for restoring skin health and elasticity compromised by sun damage.

[0073] Anti-inflammatory treatment of skin

[0074] The paper by Chen et al. (Biochem Pharmacol. 2023 Dec 9: 115979. doi: 10.1016 / j. bcp.2023.115979) explores the cooperative effects of methotrexate and electrostimulation in alleviating the relapse of psoriasiform skin inflammation by targeting memory T cells. While the primary focus is on the combined therapy's efficacy in managing psoriasis, the present inventors have concluded that it underscores the potential of electrostimulation in modulating inflammatory responses in the skin. Psoriasis is characterized by chronic inflammation driven by dysregulated immune responses, including the activation and proliferation of memory T cells. By suppressing the activity of memory T cells, electrostimulation likely plays a role in dampening the inflammatory cascade associated with psoriasis and other inflammatory skin conditions. This supports that electrostimulation according to the present invention may have broader implications in managing inflammation in dermatological disorders beyond psoriasis.

[0075] Treatment to ameliorate effects of laser treatment

[0076] While the paper by Tsolakidis et al. (Burns. 2022 Aug;48(5) : 1230-1235. doi:

[0077] 10.1016 / j. burns.2021.09.021. Epub 2021 Sep 25. PMID: 34607727) primarily investigates the effects of wireless microcurrent stimulation on improving blood flow in burn wounds, it has been realized by the present inventors that it indirectly hints at the potential of electrostimulation in mitigating laser damage. Laser treatment, particularly ablative and nonablative procedures, can induce tissue damage and inflammation in the skin. By improving blood flow and promoting tissue repair, microcurrent stimulation may have the potential to accelerate the healing process and mitigate the adverse effects of laser damage. Enhanced blood flow facilitates the delivery of oxygen and nutrients to the affected area, supporting tissue regeneration and reducing inflammation. Therefore, while not directly addressing laser damage, the findings from Tsolakidis et al.'s study support that microcurrent stimulation according to the present invention could have implications for improving wound healing and tissue recovery, including in scenarios involving laser-induced damage to the skin.

[0078] Reduction in scar and pigmentation post-surgery

[0079] In the study by Gomes et al. (Burns. 2018 May;44(3) :636-645. doi:

[0080] 10.1016 / j. burns.2017.10.006. PMID: 29402577), high-voltage electric stimulation applied to the donor site of skin grafts demonstrates potential in accelerating the healing process. While the primary focus is on wound healing, the present inventors have concluded that the reported findings indirectly suggest a role for electrostimulation in post-surgery scar management. Scar formation following surgery can lead to hypertrophic or keloid scars, which may be accompanied by hyperpigmentation. By accelerating the healing process at the donor site, electrostimulation could potentially mitigate scar formation and reduce the risk of abnormal scarring. Additionally, improved wound healing may contribute to more favourable outcomes in terms of scar appearance, texture, and pigmentation. Therefore, while not directly addressing scar pigmentation, the study supports that electrostimulation according to the present invetnion has the potential to influence scar formation and potentially contribute to more aesthetically pleasing surgical outcomes.

[0081] Hair growth

[0082] In the study by Hwang et al. (Int J Mol Sci. 2021 Apr 22;22(9) :4361. doi: 10.3390 / ijms22094361. PMID: 33921970; PMCID: PMC8122395), micro-current stimulation demonstrates potential effects in promoting hair growth, both in vitro on human hair follicle- derived papilla cells and in an animal model. Hair loss, whether due to genetic factors, hormonal imbalances, or environmental influences, is a common concern for many individuals. Micro-current stimulation has emerged as a non-invasive and promising approach to addressing this issue. By applying low-level electrical currents to the scalp, micro-current stimulation may stimulate hair follicle activity, promote blood circulation, and enhance nutrient delivery to the hair follicles. These effects can potentially prolong the anagen (growth) phase of the hair cycle, resulting in thicker, denser hair growth over time. The findings from Hwang et al.'s study have been found by the present inventors to suggest that micro-current stimulation according to the present invention holds promise as a hair loss treatment modality. By promoting the proliferation and viability of hair follicle-derived papilla cells and inducing hair growth in animal models, micro-current stimulation offers a potential non-pharmacological approach to combating hair loss. Additionally, its non-invasive nature and minimal side effects make it an attractive option for individuals seeking alternative hair restoration methods. Capability to facilitate drug delivery and / or efficacy

[0083] The papers by Luft and Ketteler (J Biomol Screen. 2015 Sep;20(8) :932-42. doi: 10.1177 / 1087057115579638. Epub 2015 Apr 7. PMID: 25851034; PMCID: PMC4543902), Guillet et al. (Chemphyschem. 2017 Oct 6; 18(19) :2715-2723. doi:

[0084] 10.1002 / cphc.201700517. Epub 2017 Sep 6. PMID: 28796918), and Gunathilake et al. (Pharm Res. 2020 Aug 30;37(9) : 178. doi: 10.1007 / sll095-020-02910-z. PMID: 32864721) have been found by the present inventors to collectively highlight the potential of electrostimulation in enhancing drug delivery and penetration. Luft and Ketteler (2015) discuss the use of electroporation, a technique involving the application of short electrical pulses to increase cell membrane permeability, for siRNA delivery in cells and tissues. By inducing transient pores in cell membranes, electroporation enables the efficient uptake of therapeutic molecules, including drugs and nucleic acids, thereby enhancing their delivery and efficacy. Similarly, Guillet et al. (2017) introduce a hydrogel / carbon-nanotube needle- free device for electrostimulated skin drug delivery. This innovative approach leverages electrostimulation to facilitate the penetration of drugs through the skin barrier, offering a non-invasive and potentially more effective alternative to traditional drug delivery methods. Furthermore, Gunathilake et al. (2020) explore electro-stimulated release of poorly water- soluble drugs from poly(lactic acid) / carboxymethyl cellulose / ZnO nanocomposite film. By applying electrical stimulation, the release of drugs from the nanocomposite film can be controlled and optimized, potentially improving drug bioavailability and therapeutic outcomes. Additionally, the study by Hwang et al. (Int J Mol Sci. 2021 Apr 22;22(9) :4361. doi: 10.3390 / ijms22094361. PMID: 33921970; PMCID: PMC8122395) provides further insight into the potential applications of electrostimulation, particularly micro-current stimulation, in promoting hair growth. While not directly related to drug delivery, this research highlights the broader impact of electrostimulation on biological processes, including tissue regeneration and cell proliferation. The findings supports that micro-current stimulation according to the present invention has the potential to stimulate hair follicle- derived papilla cells and promote hair growth, demonstrating its therapeutic potential beyond traditional drug delivery applications. Collectively, these studies underscore the versatility of electrostimulation in biomedical research and its potential as a promising tool for enhancing drug delivery, tissue regeneration, and other therapeutic interventions.

[0085] NUMBERED EMBODIMENTS

[0086] As will be appreciated from the above, the invention relates in particular to the following numbered embodiments: El. A method for cosmetic treatment of a surface area of the skin of a human subject, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current.

[0087] E2. The method according to numbered embodiment El, wherein the ionized gas molecules predominantly are negatively charged, such as negatively charged oxygen molecules.

[0088] E3. The method according to numbered embodiment El, wherein the ionized gas molecules predominantly are positively charged, such as positively charged Nitrogen molecules.

[0089] E4. The method according to any one of the preceding numbered embodiments, wherein the ionized gas molecules are derived from atmospheric air.

[0090] E5. The method according to any one of the preceding numbered embodiments, wherein the projection of ionized gas molecules establishes an electrical current between the surface area of the skin and underlying tissue.

[0091] E6. The method according to any one of the preceding numbered embodiments, wherein the electrical current is at least 0.1 pA.

[0092] E7. The method according to any one of the preceding numbered embodiments, wherein the electrical current is at most 100 pA.

[0093] E8. The method according to any one of the preceding numbered embodiments, wherein the treatment time is at least 1 second.

[0094] E9. The method according to any one of the preceding numbered embodiments, wherein the treatment time is at most 100 minutes.

[0095] E10. The method according to any one of the preceding numbered embodiments, which is carried out in combination with light treatment.

[0096] Ell. The method according to any one of the preceding numbered embodiments, wherein the ionized gas molecules are projected by a gas ion transmitting device. E12. The method according to numbered embodiment El l, wherein the device is further equipped with a camera and / or a light emitting means.

[0097] E13. The method according to any one of the preceding numbered embodiments, wherein the cosmetic treatment is selected from the group consisting of

[0098] Wrinkle depth reduction,

[0099] Skin tightening,

[0100] Skin rejuvenation,

[0101] Skin elasticity enhancement,

[0102] Skin hydration enhancement,

[0103] Collagen stimulation,

[0104] Skin peeling,

[0105] Scar revision,

[0106] Dermabrasion,

[0107] Anti-aging,

[0108] Skin discoloration reduction,

[0109] Fine line improvement,

[0110] Redness relief,

[0111] Sensitivity relief,

[0112] Exfoliation,

[0113] Acne reduction,

[0114] Youth glow induction,

[0115] Sun damage and / or photo damage prevention or repair, Anti-inflammatory treatment of skin

[0116] Treatment to ameliorate effects of laser treatment, Treatment to ameliorate effects of cryolipolysis, and Reduction in scar and pigmentation post-surgery.

[0117] E14. The method according to numbered embodiment E13, wherein the cosmetic treatment is selected from the group consisting of

[0118] Wrinkle depth reduction,

[0119] Skin tightening,

[0120] Skin rejuvenation,

[0121] Skin elasticity enhancement,

[0122] Skin hydration enhancement, Collagen stimulation, Skin peeling, Scar revision,

[0123] Dermabrasion,

[0124] Anti-aging,

[0125] Skin discoloration reduction,

[0126] Fine line improvement,

[0127] Exfoliation, and

[0128] Youth glow induction.

[0129] E15. The method according to any one of the preceding numbered embodiments, which is carried out in combination with topical administration of at least one cosmetically active substance or composition.

[0130] E16. The method according to numbered embodiment E15, wherein the delivery of the at least one cosmetically active substance or composition is enhanced by the projection of the ionized gas molecules and / or wherein the activity of the at least one cosmetically active substance is enhanced by the projection of the ionized gas molecules.

[0131] E17. The method according to numbered embodiment E15 or E16, wherein the at least on cosmetically active substance is selected from the group consisting of

[0132] Beta carotene and / or other carotenoids, Selenium or a selenium salt, Manganese or a manganese salt, One or more anti-oxidants, Glutathione, Coenzyme Q10, Lipoic acid, One or more flavonoids, One or more phenolic compounds, One or more vitamins, Iron or an iron salt, Magnesium or a magnesium salt, One or more peptides, including synthetic peptides, such as Matrixyl 3000, One or more proteins, One or more amino acids,

[0133] One or more essential oils or fats,

[0134] Platelet-rich plasma (PRP),

[0135] Collagen,

[0136] Snail mucin, Hyaluronic acid, and

[0137] Plant stem cells or other stem cells

[0138] E18. The method according to numbered embodiment E17, wherein the one or more phenolic compounds are selected from the group consisting of Quercetin, one more catechins, resveratrol, coumaric acid, one or more anthocyanins, one or more polyphenols, and one or more phytoestrogens.

[0139] E19. The method according to numbered embodiment E17, wherein the one or more vitamins are selected from the group consisting of vitamin A, retinol, B3, vitamin C, vitamin E, and vitamin D.

[0140] E20. Ionized gas molecules for use in a method of therapeutic treatment of an area of the skin of a human, the method being selected from

[0141] Redness relief,

[0142] Sensitivity relief,

[0143] Acne reduction,

[0144] Sun damage and / or photo damage prevention or repair,

[0145] Anti-inflammatory treatment of skin

[0146] Treatment to ameliorate effects of laser treatment, Treatment to ameliorate effects of cryolipolysis, and Reduction in scar and pigmentation post-surgery, wherein the method comprises that ionized gas molecules are projected onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current.

[0147] E21. The ionized gas molecules for the use according to numbered embodiment E20, wherein the method is carried out as defined in any one of numbered embodiments E2- E12.

[0148] E22. A method for therapeutic treatment of a surface area of the skin of a human subject by topic application on the skin of an active drug substance or composition comprising said substance, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current, and wherein the projection of the ionized gas molecules enhances the uptake and / or the therapeutic effect of the administered active drug substance or composition.

[0149] E23. A method for non-therapeutic cosmetic treatment of a surface area of the skin of a human subject by topic application on the skin of a cosmetically active substance or composition comprising said active substance, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current, and wherein the projection of the ionized gas molecules enhances the uptake and / or the cosmetic effect of the administered active cosmetic substance or composition.

[0150] E24. The method according to numbered embodiment E23, wherein the cosmetically active substance is selected from the group set forth in numbered embodiment E17.

[0151] E25. An active drug substance or composition comprising an active drug substance, for use in a method according to numbered embodiment E22.

[0152] E26. The method according to numbered embodiment E22 or the active drug substance or composition for the use according to numbered embodiment E25, wherein the active drug substance is selected from the group consisting of an antibiotic substance, an anti-inflammatory substance, a cytostatic or cytotoxic anti-cancer substance, a cytokine, a chemokine, a hormone, a receptor stimulant or activator, a receptor antagonist, a vasoactive substance, an analgesic, and an anaesthetic substance.

[0153] E27. The method according to numbered embodiment E22 or active drug substance or composition for the use according to numbered embodiment E25, wherein the composition is selected from the group consisting of a suspension, an o / w emulsion, a w / o emulsion, and a suspoemulsion.

[0154] E28. The method according to numbered embodiment E22 or active drug substance or composition for the use according to any one of numbered embodiments E25-27, wherein the active drug or the composition is in the form of or is incorporated in a delivery system selected from a cream, a foam, a gel, a lotion, an ointment, a paste, a powder, a shake lotion, a solid, a sponge, a tape, a tincture, a solution, and a transdermal patch.

Claims

CLAIMS1. A method for cosmetic treatment of a surface area of the skin of a human subject, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current.

2. The method according to claim 1, wherein the ionized gas molecules predominantly are negatively charged, such as negatively charged oxygen molecules (Oj), or wherein the ionized gas molecules predominantly are positively charged, such as positively charged Nitrogen molecules (NJ).

3. The method according to any one of the preceding claims, wherein the ionized gas molecules are derived from atmospheric air.

4. The method according to any one of the preceding claims, wherein the projection of ionized gas molecules establishes an electrical current between the surface area of the skin and underlying tissue, such an electrical current of at least 0.1 pA and / or an electrical current of at most 100 pA.

5. The method according to any one of the preceding claims, wherein the treatment time is at least 1 second and at most 100 minutes.

6. The method according to any one of the preceding claims, which is carried out in combination with light treatment.

7. The method according to any one of the preceding claims, wherein the ionized gas molecules are projected by a gas ion transmitting device, wherein the device preferably is further equipped with a camera and / or a light emitting means.

8. The method according to any one of the preceding claims, wherein the cosmetic treatment is selected from the group consisting of i. Wrinkle depth reduction, ii. Skin tightening, iii. Skin rejuvenation,iv. Skin elasticity enhancement, v. Skin hydration enhancement, vi. Collagen stimulation, vii. Skin peeling, viii. Scar revision, ix. Dermabrasion, x. Anti-aging, xi. Skin discoloration reduction, xii. Fine line improvement, xiii. Redness relief, xiv. Sensitivity relief, xv. Exfoliation, xvi. Acne reduction, xvii. Youth glow induction, xviii. Sun damage and / or photo damage prevention or repair, xix. Anti-inflammatory treatment of skin xx. Treatment to ameliorate effects of laser treatment, xxi. Treatment to ameliorate effects of cryolipolysis, and xxii. Reduction in scar and pigmentation post-surgery, and wherein the cosmetic treatment in particular is selected from the group consisting ofI. Wrinkle depth reduction,II. Skin tightening,III. Skin rejuvenation,IV. Skin elasticity enhancement,V. Skin hydration enhancement,VI. Collagen stimulation,VII. Skin peeling,VIII. Scar revision,IX. Dermabrasion,X. Anti-aging,XI. Skin discoloration reduction,XII. Fine line improvement,XIII. Exfoliation, andXIV. Youth glow induction.

9. The method according to any one of the preceding claims which is carried out in combination with topical administration of at least one cosmetically active substance or composition.

10. The method according to claim 9, wherein the delivery of the at least one cosmetically active substance or composition is enhanced by the projection of the ionized gas molecules and / or wherein the activity of the at least one cosmetically active substance is enhanced by the projection of the ionized gas molecules.

11. The method according to claim 9 or 10, wherein the at least on cosmetically active substance is selected from the group consisting of a. Beta carotene and / or other carotenoids, b. Selenium or a selenium salt, c. Manganese or a manganese salt, d. One or more anti-oxidants, e. Glutathione, f. Coenzyme Q10, g. Lipoic acid, h. One or more flavonoids, i. One or more phenolic compounds, j. One or more vitamins, k. Iron or an iron salt, l. Magnesium or a magnesium salt, m. One or more peptides, including synthetic peptides, such as Matrixyl 3000, n. One or more proteins, o. One or more amino acids, p. One or more essential oils or fats, q. Platelet-rich plasma (PRP), r. Collagen, s. Snail mucin, t. Hyaluronic acid, and u. Plant stem cells or other stem cells12. The method according to claim 11, wherein the one or more phenolic compounds are selected from the group consisting of Quercetin, one more catechins, resveratrol, coumaric acid, one or more anthocyanins, one or more polyphenols, and one or more phytoestrogens, orwherein the one or more vitamins are selected from the group consisting of vitamin A, retinol, B3, vitamin C, vitamin E, and vitamin D.

13. Ionized gas molecules for use in a method of therapeutic treatment of an area of the skin of a human, the method being selected fromA. Redness relief,B. Sensitivity relief,C. Acne reduction,D. Sun damage and / or photo damage prevention or repair,E. Anti-inflammatory treatment of skinF. Treatment to ameliorate effects of laser treatment,G. Treatment to ameliorate effects of cryolipolysis, andH. Reduction in scar and pigmentation post-surgery, wherein the method comprises that ionized gas molecules are projected onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current.

14. The ionized gas molecules for the use according to claim 13, wherein the method is carried out as defined in any one of claims 2-12.

15. A method for therapeutic treatment of a surface area of the skin of a human subject by topic application on the skin of an active drug substance or composition comprising said substance, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein the human subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current, and wherein the projection of the ionized gas molecules enhances the uptake and / or the therapeutic effect of the administered active drug substance or composition.

16. A method for non-therapeutic cosmetic treatment of a surface area of the skin of a human subject by topic application on the skin of a cosmetically active substance or composition comprising said active substance, the method comprising projection of ionized gas molecules onto the area of the skin so as to establish an electric current, which is driven by the projection of the ionized gas molecules, in or over the area of the skin, wherein thehuman subject optionally has at least one discharge electrode attached to an area of the body remote from the surface area of the skin so as to sustain the electric current, and wherein the projection of the ionized gas molecules enhances the uptake and / or the cosmetic effect of the administered active cosmetic substance or composition, wherein the cosmetically active substance preferably is selected from the group set forth in claim 11.

17. The method according to any one of claims 15-16, wherein the active drug substance is selected from the group consisting of an antibiotic substance, an anti-inflammatory substance, a cytostatic or cytotoxic anti-cancer substance, a cytokine, a chemokine, a hormone, a receptor stimulant or activator, a receptor antagonist, a vasoactive substance, an analgesic, and an anaesthetic substance, and wherein the composition preferably is selected from the group consisting of a suspension, an o / w emulsion, a w / o emulsion, and a suspoemulsion, and or wherein the active drug or the composition is in the form of or is incorporated in a delivery system selected from a cream, a foam, a gel, a lotion, an ointment, a paste, a powder, a shake lotion, a solid, a sponge, a tape, a tincture, a solution, and a transdermal patch.

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