Phytoecdysones for their use in the treatment of inflammatory respiratory pathologies

Abstract

The invention relates to a composition comprising at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone, for use in the treatment of an inflammatory respiratory pathology in mammals. Figure for abstract: Fig. 1

Description

Title of the invention: Phytoecdysones for their use in the treatment of inflammatory respiratory pathologies Technical field of the invention

[0001] The invention relates to the treatment of inflammatory respiratory pathologies. It falls within the field of medicines / food supplements for such treatments and relates more particularly to phytoecdysones intended for the prevention and / or treatment of inflammatory respiratory pathologies. Prior art

[0002] Phytoecdysones represent an important family of polyhydroxylated phytosterols structurally related to insect molting hormones. These molecules are produced by many plant species and participate in their defense against insect pests. The major phytoecdysone is 20-hydroxyecdysone (20E). 20E is pharmacologically active in mammals. It activates the Mas receptor, a receptor of the protective arm of the Renin Angiotensin System (Lafont et al., 2021) which induces a number of beneficial effects that have been described in preclinical studies in normal and pathological contexts.

[0003] BIO101 is an oral preparation of 20-hydroxyecdysone with a purity greater than or equal to 97%. Its preparation process is disclosed in international patent application WO2018197731 (Lafont et al. 2018). BIO101 is a new drug candidate clinically developed in the treatment of sarcopenia, in Duchenne muscular dystrophy and in COVID-19. These last two therapeutic applications are the subject of international patent applications WO2018197708 (Dilda et al. 2018) and WO2021198588 (Dilda et al. 2021). Semi-synthetic derivatives of 20-hydroxyecdysone have also been developed, as disclosed in international patent application WO2015177469 (Lafont et al. 2015), and are used for such therapeutic applications.

[0004] Asthma is a chronic inflammatory disease of the airways characterized by chronic dysregulated inflammation and exacerbated bronchial reactivity. It is the most common respiratory disease in the world, the prevalence of which has increased considerably in recent decades, reaching 5 to 10%, and affecting 339 million people worldwide (Bloom et al., 2019; Global burden of 369 diseases and injuries in 204 countries and territories, 1990-2019: a systematic analysis for the Global Burden of Disease Study 2019). Asthma is defined by variable respiratory symptoms such as shortness of breath, breathing difficulties (dyspnea), wheezing, chest tightness, and coughing, associated with chronic bronchial inflammation (Redell et al., 2015). The reduced airflow and the presence of mucus cause real difficulty in breathing. The frequency of attacks (spaced every few hours, days, or even months) and their severity vary from one patient to another. Symptoms may worsen during physical exertion or during the night.

[0005] Certain risk factors are involved in the occurrence of asthma which is the association of a genetic predisposition and environmental factors such as allergens present inside homes (mites, molds), outdoor allergens (pollens), cigarette smoke, or even air pollution (fine particles).

[0006] The pathophysiological characteristics of asthma can be divided into three interdependent components: exacerbated bronchial airway reactivity, airway remodeling, and airway inflammation.

[0007] Airway smooth muscle cells contribute to the pathogenesis of asthma, mainly through their contractile properties (Mims, 2015). Indeed, heightened bronchial airway reactivity is defined by an exaggerated response to harmless or noxious stimuli. This altered bronchial response to the environment depends on the activity of bronchial smooth muscle, the main actor of bronchoconstriction. The degree of heightened airway reactivity correlates with the severity of asthma and the need for treatment.

[0008] Beyond contractile considerations, the active involvement of airway smooth muscle in bronchial remodeling mechanisms is now established (Boulet, 2018). Airway remodeling includes alterations of the epithelium with mucus cell hyperplasia and epithelial cell fragility, subepithelial fibrosis with extracellular matrix changes, bronchial smooth muscle cell hypertrophy, and hyperplasia of blood vessels, nerves, and glands in the bronchial submucosa. Cytokines and growth factors, such as FGF or TGF[3, but also the composition of the extracellular matrix have been described as potent modifiers of the airway smooth muscle cell phenotype. These abnormalities are associated with the chronicity and severity of asthma.

[0009] Concerning the inflammation of the airways, two main pathophysiological pathways are commonly accepted: eosinophilic asthma (or type 2 asthma) and non-eosinophilic asthma. In type 2 asthma, the main cytokines involved are interleukin 4, involved in the polarization of T helper 2 lymphocytes and the switch to immunoglobulin E (IgE), interleukin 5 associated with production and trafficking of eosinophils and finally, interleukin 13 which plays a central role in airway remodeling. Conversely, non-eosinophilic asthma remains poorly understood (Habib et al., 2022; Carr et al., 2018).

[0010] The majority of asthmatic patients are well controlled by short- or long-term beta-agonist treatments as well as by inhaled corticosteroids, antileukotrienes, anticholinergics, theophylline or biotherapies such as anti-IgE (omalizumab), anti-IL-5 (mepolizumab, reslizumab, and benralizumab) or even the anti-IL-4 receptor (dupilumab).

[0011] Conventional asthma treatments primarily target two pathophysiological mechanisms: inflammation and bronchoconstriction. For example, inhaled bronchodilators (beta-2 agonists and anticholinergics) directly target airway smooth muscle cells by decreasing their contractility in order to improve airflow and limit chronic and acute symptoms. Inhaled corticosteroids, on the other hand, primarily target inflammation but also affect the contractility and proliferation of airway smooth muscle cells (Goldsmith et al., 2007; Goto et al., 2008).

[0012] However, severe asthma, the most serious degree of the disease, is uncontrolled in terms of chronic symptoms, exacerbations, permanent bronchial obstruction, which persist despite maximal management, which requires continuous use of short-acting bronchodilators despite maximal doses of inhaled corticosteroids. This leads patients to a poor quality of life as well as significant direct care costs (care visits and treatment), as well as indirect costs (workday).

[0013] Although recent biotherapies have considerably improved the course of the pathology in severe asthmatic patients, certain inflammatory endotypes, notably non-eosinophilic asthma, remain orphaned by any effective treatment (Ortega et al., 2015; Holgate et al., 2004; Castro et al., 2018).

[0014] Bronchial thermoplasty is a therapeutic innovation in the management of asthma. It is an endoscopic treatment, effective for moderate to severe asthma, allowing the reduction of smooth muscle mass by administering radiofrequency in the bronchi (Thomson et al., 2012). Although effective, this technique has short- and long-term side effects and remains a highly invasive practice.

[0015] It is accepted that severe asthma represents the clinical form in which inflammation and bronchial remodeling are intertwined. Thus, the development of new treatments acting on these mechanisms represents a major public health challenge in the sense that they could improve the therapeutic management of affected patients and modify the prognosis of the disease (Chanez et al., 2007).

[0016] The smooth muscle of the airways is distributed at the level of the tracheobronchial tree from the trachea to the terminal bronchioles. Choline innervation constitutes the principal bronchoconstrictor control thanks to acetylcholine which allows the contraction of the bronchial muscle by its interaction with muscarinic receptors whose M2 and M3 subtypes are present on the membrane of human bronchial smooth muscle cells (Kolahian and Gosens, 2012).

[0017] Nevertheless, the contraction of the smooth muscle of the respiratory tract can be caused by a large number of endogenous molecules, such as among others acetylcholine, histamine, leukotrienes, bradykinin, endothelin-1, angiotensin II, or even serotonin or 5-Hydroxytryptamine (5-HT) (Bossé, 2012).

[0018] 5-HT is one of the most studied central nervous system (CNS) neurotransmitters, known to have very diverse physiological functions outside the CNS, such as stimulation of cytokine production, vasoconstriction, cell proliferation (fibroblasts, smooth muscle cells, endothelial cells), or migration of inflammatory cells (Ménard et al., 2007; Millier et al., 2009; Delaney et al., 2011; Pakala et al., 1997; Boehme et al., 2004; Kushnir-Sukhov et al., 2006; Filip and Bader, 2009). In the lungs, it appears to be involved in chronic inflammatory diseases (Nichols and Nichols, 2008).

[0019] 5-HT exerts its effects by binding to cellular receptors that are classified into seven distinct families (5-HT1 to 5-HT7) comprising 14 subtypes, based on their structural diversity and mode of action (Kitson, 2007). The effects of 5-HT on inflammatory cells are primarily mediated by one or more of the following receptors: 5-HT 1 A, 5-HT2A, 5-HT3, 5-HT4, and 5-HT7.

[0020] Beyond its functions described above, numerous studies have demonstrated a role for 5-HT in the pathogenesis of allergy and asthma, particularly in promoting allergen-induced eosinophil recruitment, airway inflammation, heightened bronchial reactivity, and remodeling (Boehme et al., 2004; De Bie et al., 1998; Lima et al., 2007), characteristics of allergic asthma.

[0021] It has been shown that high levels of circulating serotonin are present in asthmatic patients and also that these 5-HT levels are positively correlated with the clinical status of the patients and negatively with lung function, suggesting that 5-HT may play a role in the pathophysiology of acute asthma (Lechin et al., 1994; Lechin et al., 1996).

[0022] At the level of respiratory smooth muscles, 5-HT activates both 5-HT2a and 5-HTia receptors which are responsible for contraction and relaxation respectively. muscle (Cazzola and Matera, 2000). However, it has been shown that stimulation of 5-HT1A receptors of respiratory smooth muscle cells can potentiate bronchoconstriction induced by activation of 5-HT2 receptors (Germonpré et al. 1998).

[0023] Although serotonin alone is capable of inducing bronchoconstriction, an interconnection of the serotonergic and cholinergic systems has been shown. Indeed, presynaptic serotonergic receptors are also capable of modulating (inhibiting or potentiating) the release of neurotransmitters from cholinergic and non-cholinergic nerve fibers of the airways (Germonpré et al., 1998; Mendez-Enriquez 2021).

[0024] In addition to the role of serotonin and its receptors in controlling the contractility of bronchial smooth muscle fibers, the serotonergic system also appears important in another important component of obstructive disorders: airway remodeling. Serotonergic receptors, particularly 5-HT2 receptors, play an important role in controlling the proliferation of human bronchial smooth muscle cells and in the release of the pro-fibrotic factor TGF-[31 (Lôfdhal et al. 2018).

[0025] It is important to note that certain structural alterations of the airways are common features of several respiratory disorders. Indeed, exacerbated bronchial reactivity is also observed in other pulmonary pathologies (rhinitis, chronic bronchitis, interstitial lung diseases, cystic fibrosis or even in chronic obstructive pulmonary disease (COPD)). COPD is a pathology characterized by airway obstruction generally caused by chronic bronchitis, emphysema or both. In COPD, airway obstruction results from chronic and excessive secretion of abnormal mucus, inflammation, bronchospasm and infection.There are very few treatments currently available to relieve COPD symptoms, prevent exacerbations, preserve optimal lung function and improve patients' daily activities and quality of life.

[0026] The link between serotonin and COPD has been clearly established. Indeed, significantly higher circulating serotonin levels have been observed in patients with COPD, which appears to be linked to worsening airway obstruction and mortality rates (Pirina et al. 2018; Meier et al. 2017).

[0027] It therefore appears essential to develop new pharmacological approaches to control bronchial contraction, particularly in response to serotonin, and manage the exacerbated reactivity of the airways in respiratory diseases, in order to limit as much as possible the clinical progression of patients with bronchoconstrictive disorders such as asthma or COPD. Presentation of the invention

[0028] The present invention aims to provide such a treatment. The present invention aims to overcome the aforementioned drawbacks by providing an effective treatment for inflammatory respiratory pathologies and in particular chronic inflammatory respiratory pathologies.

[0029] It has been discovered by the present inventors that such an objective is achieved by the administration of at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone, this administration unexpectedly inducing bronchodilation and preventing bronchospasm in mammals, in the context of an inflammatory respiratory pathology. The chronic administration of at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone induces a reduction in exacerbated bronchial reactivity in a murine model of severe allergic asthma. This reduction in exacerbated bronchial reactivity is observed both during a methacholine-induced bronchoconstriction test and, even more significantly, when it is induced by serotonin.Remarkably, this decrease in bronchoconstriction in response to serotonin is accompanied by the selective reduction of gene expression of a receptor of the serotonergic system. Indeed, following chronic exposure to phytoecdysone and / or a semi-synthetic derivative of 20-hydroxyecdysone, gene expression of the serotonin receptor 5-HTiA is significantly reduced in mammalian bronchi. Gene expression of the other 5-HT receptors tested (5-HTiB, 5-HT2A, 5-HT2B, 5-HT7) and of the serotonin transporter (5-HTT) at the bronchial level is not significantly affected by chronic administration of phytoecdysone and / or a semi-synthetic derivative of 20-hydroxyecdysone.

[0030] Exacerbated bronchial reactivity is associated with massive pulmonary remodeling. Interestingly, chronic administration of at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone in asthmatic mammals decreases the remodeling of the animals' airways. Indeed, in treated asthmatic mice, peribronchial and perivascular inflammation are reduced. In addition, the increase in bronchial muscle mass and epithelial barrier dysfunction associated with severe asthma appear to be less significant in treated animals. This decrease in bronchial smooth muscle cell hyperplasia would explain the decrease in exacerbated bronchial reactivity in asthmatic mice treated with chronic administration of phytoecdysone and / or semi-synthetic derivative of 20-hydroxyecdysone.

[0031] Thus, according to a first aspect, the present invention relates to a composition comprising at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone, for use in the treatment of an inflammatory respiratory pathology in mammals.

[0032] In the present description, the term "treatment" means the achievement of a desired pharmacological and physiological effect. The term "treatment", as used in the present description, includes the prevention or partial prevention of one or more of the symptoms of the disease and / or the partial or total cure of the disease and / or the total or partial disappearance of one or more of its symptoms.

[0033] In the present description, the term “at least one” means a single compound (a phytoecdysone or a semi-synthetic derivative of 20-hydroxyecdysone) or a mixture of several such compounds.

[0034] In particular embodiments, the invention further meets the following characteristics, implemented separately or in each of their technically operative combinations.

[0035] A phytoecdysone that can be used according to the invention is, for example, 20-hydroxyecdysone.

[0036] Semi-synthetic derivatives of 20-hydroxyecdysone are understood to mean in particular the known compounds which are the subject of patent application WO 2015 / 177469 in which their production by semi-synthesis is described.

[0037] Phytoecdysones and semi-synthetic derivatives of 20-hydroxyecdysone are advantageously purified to pharmaceutical grade.

[0038] According to a particular embodiment, the composition which is the subject of the present invention comprises 20-hydroxyecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone.

[0039] The 20-hydroxyecdysone used is preferably in the form of an extract of plants rich in 20-hydroxyecdysone or of a composition comprising 20-hydroxyecdysone as active agent. Extracts of plants rich in 20-hydroxyecdysone are, for example, extracts of Stemmacantha carthamoides (also called Leuzea carthamoides), Cyanotis arachnoidea, Cyanotis vaga and Pfaffia paniculata.

[0040] The extracts obtained are preferably purified to pharmaceutical grade.

[0041] In one embodiment, the 20-hydroxyecdysone is in the form of a plant extract or a part of a plant, said plant being chosen from plants containing at least 0.5% of 20-hydroxyecdysone by dry weight of said plant, said extract comprising at least 95%, and preferably at least 97%, of 20-hydroxyecdysone. Said extract is preferably purified to pharmaceutical grade.

[0042] Said extract is hereinafter called BIO101. It remarkably comprises between 0 and 0.05%, by dry weight of the extract, of impurities, such as minor compounds, sus likely to affect the safety, availability or efficacy of a pharmaceutical application of said extract.

[0043] According to one embodiment of the invention, the impurities are compounds with 19 or 21 carbon atoms, such as Rubrosterone, Dihydrorubrosterone or Poststerone.

[0044] The plant from which BIO101 is produced is preferably chosen from Stemmacantha carthamoides (also called Leuzea carthamoides), Cyanotis arachnoidea, Cyanotis vaga and Pfaffia paniculata.

[0045] According to a particular embodiment, the inflammatory respiratory pathology is a chronic inflammatory respiratory pathology.

[0046] According to a preferred embodiment, the composition which is the subject of the present invention is used as a bronchodilator in the treatment of inflammatory respiratory pathology in mammals.

[0047] The composition which is the subject of the present invention is intended to be used to induce bronchodilation in a patient suffering from an inflammatory respiratory pathology. Thus, in particular embodiments, the invention relates to a composition comprising at least one phytoecdysone and / or at least one semisynthetic derivative of 20-hydroxyecdysone, for its use in the induction of bronchodilation in the treatment of a mammal suffering from an inflammatory respiratory pathology.

[0048] In the present description, the term "bronchodilator" refers to any compound that dilates the bronchi and bronchioles and, in doing so, increases airflow to the lungs. They are useful in bronchoconstrictive disorders such as asthma, which involve airflow obstruction and bronchospasm.

[0049] In this description, the term "bronchodilation" refers to the expansion of bronchial air passages to treat or prevent a bronchoconstrictive disorder.

[0050] In this description, the term "bronchoconstrictive disorder" refers to any disorder or disease related to the reduction of the internal diameter of the bronchial passage, for example, a bronchus or bronchi, including, but not limited to, asthma, chronic obstructive pulmonary disease (COPD) and cystic fibrosis.

[0051] According to a particular embodiment, the invention relates to a composition comprising at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone, for its use in the treatment of asthma, chronic obstructive pulmonary disease or cystic fibrosis.

[0052] The composition which is the subject of the present invention is further intended to be used in the prevention or reduction of exacerbated bronchial reactivity in a patient suffering from inflammatory respiratory pathology. Thus, in particular embodiments, the invention relates to a composition comprising at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone, for use in preventing or reducing exacerbated bronchial reactivity in the treatment of a mammal suffering from an inflammatory respiratory pathology.

[0053] According to the invention, exacerbated bronchial reactivity refers to an airway abnormality that consists of an exaggerated narrowing response of the airways to numerous environmental triggers, such as allergens or exercise, but not limited to them. Exacerbated bronchial reactivity may be a functional impairment of the respiratory system caused by inflammation or remodeling of the airways. Exacerbated bronchial reactivity may be caused by collagen deposition, bronchospasm, hypertrophy of airway smooth muscle, contraction of airway smooth muscle, mucus secretion, cellular deposition, epithelial destruction, altered epithelial permeability, altered smooth muscle function or sensitivity, abnormalities of the lung parenchyma and / or infiltrative diseases in and around the airways.

[0054] Many of these causal factors may be associated with inflammation. The heightened bronchial reactivity that is targeted by treatment with the composition of the present invention is associated with airway inflammation (e.g., production of inflammatory cytokines).

[0055] In a particular embodiment, the invention relates to the composition comprising at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone for its use in the treatment of an alteration of respiratory function linked to the serotoninergic pathway in mammals suffering from an inflammatory respiratory pathology.

[0056] In a particular embodiment, the invention relates to the composition comprising at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone for its use in the treatment of an alteration of the respiratory function linked to the 5-HTiA receptor of the serotoninergic pathway in mammals suffering from an inflammatory respiratory pathology.

[0057] In a particular embodiment, the phytoecdysones are administered at a dose of between 3 and 15 milligrams per kilogram per day in humans. Phytoecdysone is understood here to mean both phytoecdysones in general, in particular 20-hydroxyecdysone and preferably in extract form, and semi-synthetic derivatives of 20-hydroxyecdysone.

[0058] Preferably, the phytoecdysones are administered at a dose of 200 to 1000 mg / day, in one or more doses, in an adult human, and a dose of 5 to 350 mg / day, in one or more doses, in a human child or infant. Phytoecdysone is understood here to mean both phytoecdysones in general, in particular 20-hydroxyecdysone and preferably in extract form, than the semisynthetic derivatives of 20-hydroxyecdysone.

[0059] In particular embodiments of the present invention, said at least one semi-synthetic derivative of 20-hydroxyecdysone is chosen from:

[0060] - a compound of general formula (I):

[0061] [Chem.l]

[0062] in which: • R1 is chosen from: a (Ci-C6)W(Ci-C6) group; a (Ci-C6)W(C1-C6)W(C1-C6) group; a (C1-C6)W(C1-C6)CO2(C1-C6) group; a (Ci-C6)A group, A representing a heterocycle optionally substituted by a group of the OH, OMe, (Ci-C6), N(Ci-C6), CO2(Ci-C6) type; a CH2Br group; W being a heteroatom chosen from N, O and S, preferably O and even more preferably S; and,

[0063] - a compound being of formula (II):

[0064] [Chem.2]

[0065] In the context of the present invention, the term "(Ci-C6)" means any alkyl group of 1 to 6 carbon atoms, linear or branched, in particular, the methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, t-butyl, n-pentyl, n-hexyl groups. Advantageously, it is a methyl, ethyl, iso-propyl or t-butyl group, in particular a methyl or ethyl group, more particularly a methyl group.

[0066] In the context of the present invention, the term heterocycle preferably means a cycle comprising 5 or 6 atoms including one or two heteroatoms (O, S or N), the remaining atoms being carbon atoms.

[0067] In a preferred embodiment of the present invention, in general formula (I): - R1 is chosen from: a group (Ci-C6)W(Ci-C6); a group (Ci-C6) W(C1-C6)W(C1-C6); a group (C1-C6)W(C1-C6)CO2(C1-C6); a group (Ci-C6)A, A representing a heterocycle optionally substituted by a group of the OH, OMe, (Ci-C6), N(Ci-C6), CO2(Ci-C6) type; W being a heteroatom chosen from N, O and S, preferably O and more preferably S.

[0068] In particular embodiments of the present invention, said at least one semi-synthetic derivative of 20-hydroxyecdysone is a compound chosen from the following compounds: No. 1: (2S,3R,5R,10R,13R,14S,17S)-2,3,14-trihydroxy-10,13-dimethyl-17-(2-morph olinoacetyl)-2,3,4,5,9,ll,12,15,16,17-decahydro-lH-cyclopenta[a]phenanthre n-6-one, No. 2: (2S,3R,5R,10R,13R,14S,17S)-2,3,14-trihydroxy-17-[2-(3-hydroxypyrrolidin-l-yl)acetyl]-10,13-dimethyl-2,3,4,5,9,ll,12,15,16,17-decahydro-lH-cyclopen ta[a]phenanthren-6-one; No. 3: (2S,3R,5R,10R,13R,14S,17S)-2,3,14-trihydroxy-17-[2-(4-hydroxy-1-piperidyl)acetyl]-10,13-dimethyl-2,3,4,5,9,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-6-one; No. 4: (2S,3R,5R,1OR,13R,14S,17S)-2,3,14-trihydroxy-17-[2-[4-(2-hydroxyethyl)-1-piperidyl]acetyl]-10,13-dimethyl-2,3,4,5,9,111,12,15,16,17-decahydro-1H-cycl openta[a]phenanthren-6-one; - No. 5: (2S,3R,5R,10R,13R,14S,17S)-17-[2-(3-dimethylaminopropyl (methyl)amino)acetyl]-2,3,14-trihydroxy-10,13-dimethyl-2,3,4,5,9,ll,12,15,l 6,17-decahydro-lH-cyclopenta[a]phenanthren-6-one; No. 6: 2-[2-oxo-2-[(2S,3R,5R,10R,13R,14S,17S)-2,3,14-trihydroxy-10,13-dimethyl-6-oxo-2,3,4,5,9,111,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-17-yl]ethyl]sulfanylacetate; No. 7: (2S,3R,5R,1OR,13R,14S,17S)-17-(2-ethylsulfanylacetyl)-2,3,14-trihydroxy-1 0,13-dimethyl-2,3,4,5,9,ll,12,15,16,17-decahydro-lH-cyclopenta[a]phenanth rene-6-one; No. 8: (2S,3R,5R,1OR,13R,14S,17S)-2,3,14-trihydroxy-17-[2-(2-hydroxyethylsulfan yl)acetyl]-10,13-dimethyl-2,3,4,5,9,ll,12,15,16,17-decahydro-lH cy-clopenta[a]phenanthren-6-one.

[0069] In embodiments, the composition which is the subject of the present invention is a pharmaceutical composition containing, as active ingredient, at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone, in a pharmaceutically acceptable vehicle.

[0070] In the present description, the term "pharmaceutically acceptable vehicle" means any vehicle useful for the preparation of a pharmaceutical composition and which is generally safe, non-toxic and neither biologically nor otherwise undesirable for the subject to be treated, in particular for mammals and in particular humans.

[0071] The vehicle of the pharmaceutical composition according to the invention can be solid, semi-solid or liquid. It can be a diluent, an adjuvant or any another classic vehicle in itself for the constitution of pharmaceutical compositions.

[0072] The pharmaceutical composition according to the invention may be in any galenic form, in particular in a form suitable for administration by parenteral, intranasal, rectal, pulmonary, intrathecal, systemic or topical route. Preferably, it is in a form suitable for oral administration. As examples of such galenic forms, which are not limiting to the invention, mention may be made of granules, powder, tablets, capsules, pills, syrup, oral solution or suspension, etc.

[0073] The administration of the compound used according to the invention to the subject to be treated can be carried out by any conventional route in itself, in particular by parenteral route, for example by subcutaneous, subdural, intravenous, intramuscular, intrathecal, intraperitoneal, intracerebral, intra-arterial or intra-lesional route; by intranasal route; by rectal route; by pulmonary route, for example by aerosol or inhalation; or even by topical route. It is preferably carried out by oral route.

[0074] Any conventional pharmaceutically acceptable salt of the compound of general formula (I) may be used according to the invention. Examples that may be mentioned are chlorides, bromides, formates, acetates, etc.

[0075] In the present description, the term "pharmaceutically acceptable salt" is understood to mean, in a conventional manner in itself, any salt of the compound of general formula (I) comprising, as counterion, a substance which does not produce any adverse, allergic or otherwise undesirable reaction when administered to a subject, in particular to a mammal.

[0076] The composition according to the invention, in its desired form, can be prepared by any conventional method in itself for the preparation of pharmaceutical compositions.

[0077] The composition according to the invention may contain one or more conventional excipients / additives in themselves for the constitution of pharmaceutical compositions, for example chosen from preservatives, sweetening agents, flavoring agents, fillers, disintegrants, wetting agents, emulsifiers, surfactants, dispersants, lubricants, stabilizers, buffers, antibacterials, antifungals, etc., or any of their mixtures; and / or any compound allowing rapid, prolonged or delayed, and / or targeted, release of the active ingredient after its administration to the subject.

[0078] The composition according to the invention may further contain one or more active ingredients other than phytoecdysones or semi-synthetic derivatives of 20-hydroxyecdysone, these active ingredients being able or not to act synergistically with said phytoecdysones or said semi-synthetic derivatives of 20-hydroxyecdysone.

[0079] The pharmaceutical composition according to the invention is preferably formulated in the form of unit doses.

[0080] The invention is also expressed in terms of a method for treating an inflammatory respiratory pathology, comprising administering to a subject in need thereof a therapeutically effective amount of a composition comprising at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone. This method may meet one or more of the characteristics described above with reference to the therapeutic use of the composition comprising at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone as a medicament.

[0081] In the present description, the expression "a subject in need thereof" means a subject suffering from or likely to be affected by an inflammatory respiratory pathology. This subject may in particular be a mammal, and in particular a human.

[0082] In the present description, the term "therapeutically effective amount" means the amount of the composition administered which, when administered to a subject to treat the disease, is sufficient to ensure such treatment of the disease. The therapeutically effective amount of the composition used according to the invention depends on several factors, such as the disease and its severity, the age, weight, etc., of the subject to be treated, the particular compound(s) of the composition used, the route and form of administration, etc. The therapeutically effective amount of the composition used according to the invention will be determined by the physician for each individual case. The composition may, for example, be administered to the subject in need thereof once, twice, or three times a day, over a long period, at regular intervals, or in a targeted manner.

[0083] The invention is also expressed in terms of a use of a composition comprising at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone for the manufacture of a medicament for the treatment of an inflammatory respiratory pathology. This use may contain one or more of the characteristics presented above, implemented separately or in each of their technically effective combinations. Brief description of the figures

[0084] The invention will be better understood on reading the following description, given by way of non-limiting example, and made with reference to the figures which represent:

[0085] [Fig-1] [Fig.l] represents the chronogram of the HDM study (meaning “House DustMite” in Anglo-Saxon terminology) in which allergic asthma model mice are treated with BIO101;

[0086] [Fig.2] [Fig.2] illustrates the contraction response curve of bronchial rings of healthy (control) or asthmatic (asthma) mice as well as the effects of the BIO101 molecule (50 mg / kg / day) on the contraction of bronchial rings in control (control+BIO101) and asthmatic (Asthma+BIO101) mice. Bronchoconstriction was induced by stimulation of cumulative doses of (A) methacholine (10 8M to 10 4M) or (B) 5-HT (10 8M to 10 4M). Values ​​are presented as means ± SEM with p<0.001#### control vs. Asthma, and Asthma vs. Asthma+BIO101, p<0.001****. ;

[0087] [Fig.3] [Fig.3] represents a histogram illustrating the gene expression of the re 5-HT receptors and its transporter in the bronchi of healthy mice (control), control mice and mice treated with BIO101 (control+BIO101), asthmatic mice (asthma) and asthmatic mice treated with BIO101 (asthma+BIO101). Values ​​are presented as means ± SEM. Values ​​are presented as means ± SEM with p<0.05.

[0088] [Fig.4] [Fig.4] represents the representative plots of the recordings in real time Figure 1. Actual response of bronchial rings from healthy mice (A: control) and mice modeling severe allergic asthma (B: asthma) following sequential administration of serotonin before and after incubation with WAY-101405, a specific 5-HT1A receptor antagonist. Scale bar: 1 minute for the x-axis and 1 / 100 for the y-axis.

[0089] [Fig.5] [Fig.5] represents representative images of lung sections Hematoxylin and Eosin stained images of healthy mice (A: control) and treated with BIO101 (B: control+BIO101), asthmatic mice (C: asthma) and asthmatic mice treated with BIO101 (D: asthma+BIO101). The scale bar represents 100 pm.

[0090] [Fig.6] [Fig.6] represents high magnification images, representative of Hematoxylin and Eosin-stained lung sections from healthy mice (A: control) and treated with BIO101 (B: control+BIO101), asthmatic mice (C: asthma), and asthmatic mice treated with BIO101 (D: asthma+BIO101). The scale bar represents 100 μm. Description of the embodiments

[0091] In the present description, n corresponds to the sample size and p corresponds to the “p-value” used to quantify the statistical significance of a result.

[0092] A Mann-Whitney test was performed for comparisons between two groups. A Two-way ANOVA test was used for multiple comparisons of bronchial contractility studies. Data analysis was performed using GraphPad Prism 9 software (GraphPad Software, Inc., La Jolla, CA, USA). The invention will be described below in the particular context of one of its preferred, non-limiting fields of application.

[0093] 1. Description of the study:

[0094] 7-week-old mice are cutaneously sensitized to dust mite extracts (HDM for House DustMite) at D0, 7, 14 and 21. After this phase of cutaneous sensitization to the allergen, the mice receive the allergen via the nasal route (challenge phase) at D27, 28, 29, 34, 35 and 36 to induce bronchial exacerbation. This model of severe allergic asthma has been characterized as mimicking human pathology (Dilasser et al. Thorax 2021).

[0095] BIO101 (lot RL11T1204A0) was administered to mice in drinking water 7 days a week at a dose of 50 mg / kg / day. The drinking water containing BIO101 was replaced twice a week until the animals were sacrificed.

[0096] 2. Biological activity of BIO101 in a mouse model of severe allergic asthma

[0097] a. Analysis of the effects of BIO101 treatment on bronchoconstriction

[0098] The evaluation of the effect of chronic administration of BIO101 treatment on the ex vivo contraction of murine bronchial rings was carried out in an isolated organ chamber (isometric tension measurement, Mulvany myograph, DMT, Hinnerup, Denmark) (André-Grégoire et al., 2018).

[0099] The bronchi are collected, cleaned, cut into rings and installed in the Mulvany myograph. The rings are pre-tensioned and then subjected to stimulation with 60 mM KC1, until the amplitude of the contractile responses is stabilized (2 or 3 stimulations) before the initiation of each experimental protocol.

[0100] At D38 after the start of the protocol ([Fig.l]), the bronchi of healthy non-asthmatic mice (control; n>8), healthy non-asthmatic mice treated with BIO101 (control+BIO101; n=8), asthmatic mice (Asthma; n>8) and asthmatic mice treated with BIO101 (Asthma+BIO101; n>8) were sampled. Bronchoconstriction was induced by stimulation of cumulative doses of methacholine (107 M to 10 4 M) or serotonin (10 7 M to 10 4 M).

[0101] The experiments were performed on 2 bronchial rings of each mouse. The BIO101 molecule was added at a concentration of 10 5M in the isolated organ chambers containing the bronchi of the treated mice. This allowed to maintain the treatment of the tissues throughout the experiment. It is noteworthy that the acute administration of BIO101 in the tank containing the bronchial ring does not modify the contractile response to methacholine (0.41 ± 0.25mN) compared to the internal control without BIO101 (0.37 ± 0.22mN; p=ns) nor to serotonin (0.29 ± 0.14mN) compared to the internal control without BIO101 (0.25 ± 0.12mN; p=ns) (previous results obtained, not presented here).

[0102] As expected, in asthmatic mice, bronchoconstrictions induced by methacholine and serotonin are significantly higher compared to those in healthy mice (p<0.0001 for each condition), which attests that sensitization to dust mites induced exacerbated bronchial reactivity ([Fig.2]).

[0103] In asthmatic mice, chronic treatment with BIO101 significantly decreases methacholine-induced bronchoconstriction in asthmatic mice (Asthma vs. Asthma+BIO101; p<0.0001). However, the treatment does not prevent exacerbated bronchial reactivity of the airways and does not achieve a level of bronchoconstriction comparable to that of healthy mice (Asthma+BIO101 vs. Control; p=0.0025).

[0104] Similarly, chronic treatment with BIO101 significantly decreases serotonin-induced bronchoconstriction in asthmatic mice (Asthma vs. Asthma+BIO101; p<0.0001). Remarkably, chronic treatment with BIO101 is observed to induce serotonin-induced bronchoconstriction comparable to that observed with bronchi of healthy mice (Asthma+BIO101 vs. Control; p=0.9997) demonstrating that the treatment completely prevents exacerbated bronchial reactivity of the airways.

[0105] b. Analysis of the effect of chronic treatment with BIO101 on the gene expression of 5-HT receptors and transporters in the bronchi

[0106] At D38, the bronchi of healthy non-asthmatic mice (control; n=10), non-asthmatic control mice treated with BIO101 (controls+BIO101; n=10), asthmatic mice (Asthma; n=10) and asthmatic mice treated with BIO101 (Asthma+BIO101; n=10) were collected. After RNA extraction from these tissues (Trizol©, ThermoFisher cat. 15596018), an analysis of the expression of serotonin receptors (5-HT1A, 5-HT1B, 5-HT2A, 5-HT2B and 5-HT7) and the serotonin transporter (5HTT) was evaluated by real-time PCR (TaqMan™, Universal PCR MasterMix, ThermoFisher cat. 4304437). The list of references of the commercial ThermoFisher Scientific tests used for real-time PCR that allowed obtaining the results for each of the genes is given in Table 1 below:

[0107] [Tableauxl] Gene Symbol Reference Test Htt Mm01213820 ,m1 Htr7 Mm00434133_m1 Htr2a Mm00555764_m1 Htr2b Mm00434123_m1 Htrla Mm00434106_s1 Htr1 b Mm00439377 s1 Gapdh Mm99999915_g1 Hprt Mm03024075_m1

[0108] Chronic treatment of mice with BIO101 induces a significant and selective decrease in the expression of the 5-HT1A receptor gene in the bronchi ([Fig.3]). Indeed, the gene expression of the 5-HT1A receptor decreases by -49% ± 12% (p<0.05) in healthy mice treated with BIO101 (control+BIO101) and even more significantly in asthmatic mice treated with BIO101 (asthma+BIO101) (-69 ± 3%; p<0.05).

[0109] Treatment with BIO101 does not induce any modification of other receptors or the serotonin transporter, whether in healthy or asthmatic mice (5-HT1B, 5-HT2A, 5-HT2B and 5-HT7 and 5-HTT).

[0110] c. Confirmation of the involvement of the 5-HT1A receptor in serotonin-induced bronchoconstriction.

[0111] Real-time recordings of the bronchial response of control and asthmatic mice following sequential administration of serotonin at different concentrations (10 8M to 104M) were performed. Following this recording, the bronchial rings were incubated for 30 minutes with WAY-101405, a selective 5-HT1A receptor antagonist ([Fig.4]).

[0112] As expected, serotonin induces bronchoconstriction in the bronchial rings of healthy (control) and asthmatic mice. Bronchoconstriction in the bronchi of asthmatic mice ([Fig.4], B) is greater than that of healthy mice ([Fig.4], A). Serotonin-induced bronchoconstriction is completely inhibited by the 5-HT1A receptor antagonist (WAY-101405) in control and asthmatic mice ([Fig.4], A and B).

[0113] This result suggests that serotonin-dependent bronchoconstriction is primarily mediated by activation of the 5-HT1A receptor.

[0114] All of these results strongly suggest that the selective reduction in the expression of 5-HT1A receptors induced by chronic treatment with BIO101 could significantly contribute to the prevention of exacerbated bronchial reactivity of the airways in an inflammatory pathological context.

[0115] d. Histopathological analysis of the effects of chronic treatment with BIO101 on the lungs

[0116] Histological analysis of lung sections stained with hematoxylin and eosin shows that the exacerbated bronchial reactivity of the airways of mice sensitized to dust mites (asthma, [Fig.5] in C and [Fig.6] in C) is associated with massive lung remodeling characterized by mucus production, strong cellular infiltration of the airways, epithelial cell hypertrophy, as well as smooth muscle cell hyperplasia, compared to the lung of healthy mice ([Fig.5] in A and [Fig.6] in A).

[0117] In healthy mice (control), treatment with BIO101 has no effect on lung histology (([Fig.5] in B and [Fig.6] in B). In contrast, in asthmatic mice, treatment with BIO101 reduced peribronchial and perivascular inflammation (([Fig.5] in D and [Fig.6] in D). In addition, the increase in bronchial muscle mass and epithelial barrier dysfunction associated with severe asthma appear to be less significant in animals that received chronic treatment with BIO101.

[0118] Conclusion

[0119] These results demonstrate the interest of using BIO101 treatment in the context of inflammatory respiratory pathologies in order to reduce exacerbated bronchial reactivity. Indeed, BIO101 shows significant beneficial effects in a murine model of severe allergic asthma, in particular at the level of bronchoconstriction induced by methacholine and serotonin. Chronic administration of BIO101 induces a significant decrease in the 5-HT1A receptor in the bronchi as well as a decrease in inflammation and remodeling of the airways.

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Claims

Claims

1. Composition comprising at least one phytoecdysone and / or at least one semi-synthetic derivative of 20-hydroxyecdysone, for use in inducing bronchodilation and / or in preventing or reducing exacerbated bronchial reactivity in mammals suffering from an inflammatory respiratory pathology, said semi-synthetic derivative of 20-hydroxyecdysone being chosen from: - a compound of general formula (I): [Chem.l] in which: R1 is chosen from: a (Ci-C6)W(Ci-C6) group; a (Ci-C6)W(Ci-C6)W(Ci-C6) group; a (CrC 6)W(Ci-C6)CO2(Ci-C6) group; a (Ci-C6)A group, A representing a heterocycle optionally substituted by a group of the OH, OMe, (Ci-C6), N(Ci-C6), CO2(Ci-C6) type; a CH2Br group; W being a heteroatom chosen from N, O and S, preferably O and even more preferably S; and, - a compound having formula (II): [Chem. 2]

2. Composition for use according to claim 1, wherein the inflammatory respiratory pathology is a chronic inflammatory respiratory pathology.

3. Composition for use according to any one of claims 1 to 2, in which the inflammatory respiratory pathology is asthma, chronic obstructive pulmonary disease or cystic fibrosis.

4. Composition for use according to any one of claims 1 to 3, wherein the composition is used in the treatment of an alteration of respiratory function linked to the serotonergic pathway in mammals suffering from an inflammatory respiratory pathology.

5. Composition for use according to claim 4, wherein the composition is used in the treatment of an alteration of respiratory function linked to the 5-HTiA receptor of the serotonergic pathway in mammals suffering from an inflammatory respiratory pathology.

6. Composition for use according to any one of claims 1 to 5, comprising 20-hydroxyecdysone.

7. Composition for use according to claim 6, wherein the 20-hydroxyecdysone is in the form of a plant extract or a part of a plant, said plant being chosen from plants containing at least 0.5% of 20-hydroxyecdysone by dry weight of said plant, said extract comprising at least 95%, and preferably at least 97%, of 20-hydroxyecdysone.

8. Composition for its use according to claim 7, remarkably comprising between 0 and 0.05%, by dry weight of the extract, of impurities likely to affect the safety, availability or efficacy of a pharmaceutical application of said extract.

9. Composition for use according to any one of claims 7 to 8, wherein the plant is selected from Stemmacantha carthamoides, Cyanotis arachnoidea, Cyanotis vaga and Pfaffia pa-niculata.

10. Composition for use according to any one of claims 1 to 9, in which in the general formula (I): R1 is chosen from: a (Ci-C6)W(Ci-C6) group; a (C1-C6)W(C1-C6)W(C1-C6) group; a (C1-C6)W(C1-C6)CO2(C1-C6) group; a (Ci-C6)A group, A representing a heterocycle optionally substituted by a group of the OH, OMe, (Ci-C6), N(C rC6), CO2(CrC6) type; W being a heteroatom chosen from N, O and S, preferably O and more preferably S.

11. Composition for use according to any one of claims 1 to 10, wherein said compound of general formula (I) is chosen from: - No. 1: (2S,3R,5R,10R,13R,14S,17S)-2,3,14-trihydroxy-10,13-dimethyl-17-(2-morpholinoacetyl)-2,3,4,5,9,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-6-one; - n°2 : (2S,3R,5R,10R,13R,14S,17S)-2,3,14-trihydroxy-17-[2-(3-hydroxypyrro lidin-l-yl)acetyl]-10,13-dimethyl-2,3,4,5,9,ll,12,15,16,17-decahydro-l H-cyclopenta[a]phenanthren-6-one; - n°3 : (2S,3R,5R,10R,13R,14S,17S)-2,3,14-trihydroxy-17-[2-(4-hydroxy-l-pi peridyl)acetyl]-10,13-dimethyl-2,3,4,5,9,ll,12,15,16,17-decahydro-lH-cyclopenta[a]phenanthren-6-one; - n°4: (2S,3R,5R,10R,13R,14S,17S)-2,3,14-trihydroxy-17-[2-[4-(2-hydroxyeth yl)-l-piperidyl]acetyl]-10,13-dimethyl-2,3,4,5,9,ll,12,15,16,17-decahy dro-lH-cyclopenta[a]phenanthrene-6-one; - No. 5: (2S,3R,5R,10R,13R,14S,17S)-17-[2-(3-dimethylaminopropyl(methyl)a mino)acetyl]-2,3,14-trihydroxy-10,13-dimethyl-2,3,4,5,9,11,12,15,16,17 -decahydro-lH-cyclopenta[a]phenanthren-6-one; - No. 6: 2-[2-oxo-2-[(2S,3R,5R, 10R, 13R, 14S, 17S)-2,3,14-trihydroxy-10,13-dim ethyl-6-oxo-2,3,4,5,9,ll,12,15,16,17-decahydro-lH-cyclopenta[a]phena nthren-17-yl]ethyl]sulfanylacetate; - No. 7: (2S,3R,5R,10R,13R,14S,17S)-17-(2-ethylsulfanylacetyl)-2,3,14-trihydr oxy-10,13-dimethyl-2,3,4,5,9,ll,12,15,16,17-decahydro-lH-cyclopenta[ a]phenanthren-6-one; - n°8: (2S,3R,5R,10R,13R,14S,17S)-2,3,14-trihydroxy-17-[2-(2-hydroxyethyls sulfanyl)acetyl]-10,13-dimethyl-2,3,4,5,9,ll,12,15,16,17-decahydro-lH-cyclopenta[a]phenanthren-6-one.

Images