Brassica oleracea vegetable powder or composition containing it to regulate metabolic syndrome
Brassica oleracea vegetable powder, rich in polyphenols, addresses metabolic syndrome by inhibiting fat production and accumulation, effectively reducing cholesterol and triglycerides and improving liver health, while being safe for long-term use.
Patent Information
- Application Number
- FR2023007120
- Authority / Receiving Office
- FR · FR
- Patent Type
- Utility models
- Current Assignee / Owner
- Filing Date
- 2023-07-04
- Publication Date
- 2025-08-01
- Estimated Expiration
- 2033-07-04
AI Technical Summary
Existing diets rich in low-fiber, high-sugar, and high-fat meals contribute to metabolic syndrome, a condition associated with abnormal fat metabolism, leading to health risks such as high blood pressure, diabetes, and heart disease, with Brassica oleracea vegetables not previously reported to effectively regulate blood lipids, blood sugar, or body weight.
A Brassica oleracea vegetable powder, rich in polyphenol compounds, is prepared through extraction and drying processes, and administered to inhibit fat production and accumulation, formulated into foods, supplements, or drugs to regulate metabolic syndrome.
The Brassica oleracea vegetable powder effectively reduces cholesterol and triglyceride levels, inhibits fat accumulation in the liver, and prevents or improves metabolic syndrome-related diseases like fatty liver and diabetes, with no toxicity to normal cells.
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Abstract
Description
Title of the invention: Brassica oleracea vegetable powder or composition containing it for regulating metabolic syndrome BACKGROUND OF THE INVENTION 1. Technical field
[0001] The present invention relates to a second use of Brassica oleracea vegetables, in particular a composition containing Brassica oleracea vegetables for regulating metabolic syndrome.
[0002] 2. Description of the related state of the art
[0003] As Western and refined meals are popular among people today, most of these meals are low-fiber, high-sugar, and high-fat meals, leading to an increase in the proportion of patients suffering from metabolic syndrome. The so-called metabolic syndrome is a general term for abnormalities in blood pressure, blood lipids, blood sugar, and waist circumference, which indicate that an individual's health is at high risk. If patients with metabolic syndrome do not actively control their diet, change their lifestyle habits, and increase the frequency of physical exercise, they will face a higher risk of high blood pressure, diabetes, hyperlipidemia, heart disease, and stroke than people without metabolic syndrome.According to data published by the World Health Organization, adequate consumption of vegetables and fruits can prevent chronic diseases such as heart disease, cancer, diabetes and obesity.
[0004] Brassica oleracea belongs to the genus Brassica in the cruciferous family, in which broccoli (Brassica oleracea var. italica) and kale (Brassica oleracea var. sabellica L.) are widely consumed vegetables. Brassica oleracea vegetables, rich in vitamin C and dietary fiber as well as various nutrients and phytochemicals, such as indole-3-carbinol, 3,3'-diindolylmethane, sulforaphane and the like, have strong antioxidant capacity and the effect of inhibiting tumor cells. However, no one has yet reported on the effectiveness of Brassica oleracea vegetables in regulating blood lipids, blood sugar or body weight. BRIEF SUMMARY OF THE INVENTION
[0005] A main object of the present invention is to provide a Brassica oleracea vegetable powder or a composition containing it for use in regulating metabolic syndrome or a metabolic syndrome-related disease. Due to the fact that the Brassica oleracea vegetable powder obtained by the special method disclosed in the present invention has the effect of inhibiting the production and accumulation of fat, Brassica oleracea vegetable powder can effectively regulate metabolic syndrome or metabolic syndrome-related disease.
[0006] Another objective of the present invention is to provide a Brassica oleracea vegetable powder or a composition containing the same for use in regulating metabolic syndrome or a metabolic syndrome-related disease, wherein the Brassica oleracea vegetable powder having high safety for individuals and activity in regulating metabolic syndrome or a metabolic syndrome-related disease can be provided such that individuals do not have health problems after taking the Brassica oleracea vegetable powder for a long period of time.
[0007] Therefore, in order to achieve the above-mentioned objectives, the present invention discloses a Brassica oleracea vegetable powder or a composition containing the same for use in regulating metabolic syndrome or a metabolic syndrome-related disease, and the Brassica oleracea vegetable powder contains at least one polyphenol compound. By administering to an individual the composition for regulating metabolic syndrome or a metabolic syndrome-related disease, it is possible to effectively achieve the effect of preventing or improving metabolic syndrome or a metabolic syndrome-related disease.
[0008] The composition may be a food, a dietary supplement, a functional food or a drug, and the composition may be prepared in various dosage forms or in various forms, such as powders, lozenges, capsules, beverages, biscuits, candies, infuser bags and the like.
[0009] A metabolic syndrome disease is a disease related to abnormal fat metabolism in the liver, such as fatty liver, liver cancer, hyperlipidemia, diabetes and the like.
[0010] In one embodiment of the present invention, the Brassica oleracea vegetable powder is prepared from a Brassica oleracea vegetable raw material by extraction and drying processes, wherein the Brassica oleracea vegetable raw material is broccoli or kale.
[0011] BRIEF DESCRIPTION OF THE DIFFERENT VIEWS OF THE DRAWINGS
[0012] [Fig-1] presents the results of detection and analysis of triglyceride content in HepG2 cell groups in high oil and high glucose model after different sample treatments.
[0013] [Fig.2] shows the results of testing and analysis of total cholesterol content in HepG2 cell groups in a high-oil, high-glucose model after different sample treatments.
[0014] [Fig.3] presents the results of detection and analysis of lipid content in HepG2 cell groups in a high-fat model after different sample treatments.
[0015] [Fig.4] shows the results of testing and analyzing the lipid content in 3T3-L1 cell groups after different sample treatments. DETAILED DESCRIPTION OF THE INVENTION
[0016] The present invention relates to a Brassica oleracea vegetable powder or a composition containing the same for use in regulating metabolic syndrome or a metabolic syndrome-related disease. Specifically, the Brassica oleracea vegetable powder has an activity of inhibiting the production and accumulation of fats. Therefore, administering an effective amount of Brassica oleracea vegetable powder to an individual can reduce the cholesterol and triglyceride contents of cells and improve or prevent metabolic syndrome or a metabolic syndrome-related disease.
[0017] In embodiments, the Brassica oleracea vegetable powder can be prepared into a composition for regulating metabolic syndrome or a metabolic syndrome-related disease. The composition is a food, a dietary supplement, or a drug; and the composition can be prepared into various dosage forms depending on the use needs, such as lozenges, capsules, powders, liquids, and the like.
[0018] The individual herein refers to a person having a usual diet rich in oil, rich in glucose and / or rich in fat.
[0019] In embodiments of the present invention, the Brassica oleracea vegetable powder contains at least one polyphenol compound. The Brassica oleracea vegetable powder is an extraction powder prepared from a Brassica oleracea vegetable raw material by extraction, drying and the like methods. Commonly, the drying method includes low-temperature hot air drying, high-temperature hot air drying and freeze-drying.
[0020] The raw vegetable material of Brassica oleracea is broccoli or kale.
[0021] In embodiments of the present invention, a disease related to the syndrome Metabolic syndrome is a disease related to abnormal fat metabolism in the liver. Specifically, the disease related to metabolic syndrome is fatty liver disease, liver cancer, or other liver diseases.
[0022] In order to illustrate the technical characteristics and effects of the present invention, experimental examples will be given below, and they will be described as follows with reference to the drawings.
[0023] The cells used in the following experimental examples are all dis commercially available, so that no additional patent filing is necessary.
[0024] Experimental Example 1: Preparation of a Brassica oleracea vegetable powder
[0025] Organic broccoli and kale were taken and submitted separately to the pre-cooling, washing, cutting, blanching, drying, crushing and grinding processes to obtain broccoli powder and kale powder.
[0026] Experimental Example 2: Preparation of a Vegetable Solution
[0027] The broccoli powder and kale powder obtained in Experimental Example 1 were added to deionized water to prepare 1 wt% and 5 wt% suspension solutions respectively. The suspension solutions were extracted by ultrasonic vibration for 30 min and then centrifuged. The supernatant was filtered with a 0.22 μm filter membrane to obtain a broccoli extract and a kale extract.
[0028] Experimental example 3: Test on the capacity to trap free radicals
[0029] 0.2 mL of solution was taken in 5% by weight methanol (concentration of 100-1000 pg / mL) was added to 0.8 mL of freshly prepared 0.25 mM DPPH (1,1-diphenyl-2-picrylhydrazyl) methanol solution. Broccoli extract and kale extract were separately added to the resulting solution, then mixed well and reacted for 30 min. The absorbance values of the reaction solutions were determined at the wavelength of 517 nm to calculate the free radical scavenging capacity of each vegetable solution as follows:
[0030] Free radical scavenging capacity determined by DPPH (%) = (absorbance value of control group - absorbance value of samplej / absorbance value of control groupxl00%.
[0031] The above detection and analysis results show that the free radical scavenging capacity determined by DPPH of broccoli is 50.3% and the free radical scavenging capacity determined by DPPH of kale is 67.4%.
[0032] Experimental example 4: Test on the total polyphenol content
[0033] In this experimental example, the total polyphenol content in broccoli powder and kale powder was determined by the Folin-Ciocaltea method. In the test, the wavelength was 750 nm; the gallic acid solution was used to make the standard calibration curve; the total polyphenol content of each sample was expressed in milligrams of gallic acid equivalents per 100 grams of dry matter weight.
[0034] According to the test and analysis results, the total polyphenol content of broccoli powder is 2.62 mg / g and the total polyphenol content of kale powder is 4.5 mg / g. It can be seen that broccoli and kale all contain both have an extremely high content of total polyphenols. Furthermore, based on the positive correlation between polyphenol content and free radical scavenging capacity and in combination with the results of Experimental Example 3, it can be concluded that broccoli and kale are vegetables with high free radical scavenging activity.
[0035] Experimental Example 5: Cellular Test (I)
[0036] HepG2 cells (BCRC RM60025) were inoculated into a culture tray with an amount of 4x105 cells / well, and incubated in DMEM (Dulbecco's Modified Eagle's Medium) with 10 wt% fetal bovine serum and 1 wt% P / S (penicillin and streptomycin) in a 37°C incubator in the presence of 5 wt% CO2 for 24 h. When the cells grew to about 7-80% of full growth, the cells were treated with 300 µM palmitic acid and 100 mM fructose. The experimental groups were treated together with 10 µM curcumin, 1 wt% broccoli extract and 1 wt% kale extract for 24 h. Then, triglyceride and total cholesterol detection kits were used to analyze the triglyceride content and total cholesterol content in cells, and the results are shown in Figures 1 and 2.
[0037] The results in Figures 1 and 2 show that, compared with the control group, the experimental groups exhibited a decrease of approximately 46% in cholesterol content in cells co-incubated with broccoli or kale, a decrease of approximately 42% in triglyceride content in cells co-incubated with broccoli extract, and a decrease of approximately 44% in triglyceride content in cells co-incubated with kale extract.
[0038] These results show that Brassica oleracea vegetables can effectively inhibit lipid synthesis activity in the liver. Therefore, administering an effective amount of Brassica oleracea vegetable powder or a composition containing Brassica oleracea vegetable powder to an individual with a habitual diet high in oil and / or high in fat can inhibit the synthesis and metabolism of excess fatty acids and fructose into triglycerides in the liver, thereby preventing or improving hepatic steatosis or other diseases caused by abnormal fat metabolism.
[0039] Experimental Example 6: Cellular Test (II)
[0040] HepG2 cells (BCRC RM60025) were inoculated into a culture tray with an amount of 6x104 cells / well, and incubated in DMEM (Dulbecco's Modified Eagle's Medium) with 10 wt% fetal bovine serum and 1 wt% P / S in a 37°C incubator in the presence of 5 wt% CO2 for 24 h. When the cells grew to about 7-80% of full growth, the cells were treated with 16 µM free fatty acid. The experimental groups were treated together with 25 μM curcumin, 1 wt% broccoli extract, and 1 wt% kale extract for 24 h. Then, the cell groups were respectively subjected to Oil Red O staining, and the accumulation of oil droplets in each cell group was analyzed. The results are shown in [Fig.3],
[0041] The results in [Fig.3] show that in the high-fat-induced fatty liver cell model, HepG2 cells in the control group produced significant fat accumulation in the liver; treatment with Brassica oleracea extract can effectively slow down the fat accumulation in liver cells. If the cells are treated with kale extract or broccoli extract at the same time as high-fat treatment, the fat accumulation in liver cells can be effectively slowed down.
[0042] The comprehensive analysis of the results of Experimental Examples 5 and 6 shows that the Brassica oleracea vegetables and their powders disclosed in the present invention have an activity of inhibiting the accumulation of fats in the liver. This means that, by administering an effective amount of the Brassica oleracea vegetables and their powders to an individual having a habitual diet rich in fat, it is possible to effectively treat or improve hepatic steatosis or other diseases caused by abnormal fat metabolism.
[0043] Experimental Example 7: Cellular Test (III)
[0044] 3T3-L1 cells (BCRC 60159) were inoculated and incubated in a 12-well culture tray with an amount of 1.5-2x105 cells / well or in a 24-well culture tray with an amount of 5-7x104 cells / well. The cell culture tray should be pretreated with 0.1 wt% gelatin O / N before incubation. The cells were incubated for about 2-3 days until the cells reached their maximum growth, and then a differentiation reagent (0.5 mM methyl-isobutylxanthine + 1 qM dexamethasone + 5 qg / mL insulin + 2 qM Rosiglitazone, prepared in culture medium) was added to induce differentiation for 48 h.48 h later, the culture medium was removed, 5 qg / mL insulin (prepared in culture medium) was added, and the experimental groups were treated together with 25 qM resveratrol, 25 qM curcumin, 1 wt% broccoli extract, and 1 wt% kale extract and incubated for 6 days, and the culture medium containing 5 qg / mL insulin was replaced every 2 days. After cell culture was completed, the cells were subjected to Oil Red O staining, and the statistical analysis results are shown in [Fig.4]. .
[0045] The results in [Fig.4] show that, compared with the control group, the experimental groups exhibit a decrease in the lipid content of the cells. treated with broccoli extract or kale extract, indicating that broccoli extract or kale extract can inhibit the formation of oil droplets. In addition, the effect of broccoli extract on inhibiting oil droplet formation is better than that of resveratrol or curcumin.
[0046] Experimental Example 8: Cellular Test (III)
[0047] Normal cells (HS68 cells), liver cancer cells (HepG2 cells), cervical cancer cells (HeLa cells), and lung adenocarcinoma cells (A549 cells) were co-cultured with different concentrations (1 wt%, 3 wt%, 5 wt%, 10 wt%) of broccoli extract or kale extract, and the survival rates of the cells were detected. The results are shown in Table 1 below.
[0048] The results in Table 1 show that the broccoli extract or kale extract does not produce cytotoxicity to normal cells even at a high dose (10% by weight), but has a toxic effect on liver cancer cells. It is found that the broccoli extract or kale extract disclosed in the present invention is not only non-toxic to normal cells, but also has an anticancer effect.
[0049] [Table 1] Results of cell survival rate analysis Kale Broccoli Dose 1% 3% 5% 10% 1% 3% 5% 10% Cell Survival Rate Normal Cells (HS68) 112% 112% 117% 125% 109% 99% 91% 83% Liver Cancer Cells (HepG2) 88% 73% 69% 66% 78% 69% 67% 65% Cervical Cancer Cells (HeLa) 92% 78% 73% 57% 91% 76% 66% 43% Lung Adenocarcinoma Cells (A549) 82% 67% 64% 60% 90% 73% 64% 54%
Claims
Claims
1. A Brassica oleracea vegetable powder or composition containing it for use in regulating metabolic syndrome or a metabolic syndrome-related disease, the Brassica oleracea vegetable powder containing at least one polyphenol compound.
2. Brassica oleracea vegetable powder or composition containing it for use in regulating metabolic syndrome or a metabolic syndrome-related disease, according to claim 1, characterized in that the Brassica oleracea vegetable powder is prepared from a Brassica oleracea vegetable raw material
3. Brassica oleracea vegetable powder or composition containing it for use in regulating metabolic syndrome or a metabolic syndrome-related disease, according to claim 2, characterized in that the Brassica oleracea vegetable raw material is broccoli or kale.
4. Brassica oleracea vegetable powder or composition containing it for use in regulating metabolic syndrome or a metabolic syndrome-related disease, according to claim 1, characterized in that the composition is a food.
5. Brassica oleracea vegetable powder or composition containing it for use in regulating metabolic syndrome or a disease related to metabolic syndrome, according to claim 1, characterized in that the composition is a food supplement.
6. Brassica oleracea vegetable powder or composition containing it for use in regulating metabolic syndrome or a metabolic syndrome-related disease, according to claim 1, characterized in that the metabolic syndrome-related disease is a disease related to abnormal fat metabolism in the liver.
7. Brassica oleracea vegetable powder or composition containing it for use in regulating metabolic syndrome or a metabolic syndrome-related disease, according to claim 6, characterized in that the metabolic syndrome-related disease is fatty liver.
8. A Brassica oleracea vegetable powder or composition containing it for use in regulating metabolic syndrome or a metabolic syndrome-related disease, according to claim 6, ca- characterized by the fact that the disease linked to metabolic syndrome is liver cancer.