ANTIBIOTIC SUSCEPTIBILITY TEST REAGENT DISC STRUCTURE

The disc structure addresses inefficiencies in sample and reagent distribution by uniformly distributing them, enhancing the consistency and speed of antibiotic susceptibility tests.

FR3168602A3Pending Publication Date: 2026-05-22BIOGUARD CORP
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Patent Information

Authority / Receiving Office
FR · FR
Patent Type
Utility models
Current Assignee / Owner
BIOGUARD CORP
Filing Date
2025-07-23
Publication Date
2026-05-22

AI Technical Summary

Technical Problem

Current antibiotic susceptibility testing methods are inefficient in uniformly distributing samples and reagents, leading to inconsistent results and increased testing time.

Method used

A disc structure with a central injection part, microflow channels, reaction receptacles, overflow channels, and overflow sumps is designed to uniformly distribute samples and pre-loaded reagents for antibiotic susceptibility tests, ensuring consistent and rapid testing.

Benefits of technology

The structure enables uniform distribution of samples and reagents, reducing testing time and improving the consistency of antibiotic susceptibility test results.

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Abstract

The invention relates to a reagent disc structure for antibiotic susceptibility testing, comprising a test holder (1), at least two support and positioning parts (112), a central injection portion (2), several microflow channels (3), several reaction receptacles (4), several preloaded reagents (41) for antibiotic susceptibility testing, several overflow channels (5), several overflow sumps (6), several narrowing portions (51), and several vent holes (7). Before packaging, the reaction receptacles (4) of the test holder (1) are preloaded with lyophilized preloaded reagents (41). A user can directly position the test holder (1) in a test device after injecting the sample into the central injection portion (2).During activation, the sample is subjected to centrifugal force, causing it to flow uniformly through the microflow channels (3) into the reaction receptacles (4). Figure for the abstract: 1.
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Description

at least two support and positioning parts (112), which are formed in the test support (1) to support the test support (1); a central injection part (2), which is arranged between the support and positioning parts (112) for the introduction of a sample and for limiting the insertion depth of an injection tool by means of the support and positioning parts (112); a plurality of microflow channels (3), which are arranged radially, at intervals, next to the central injection part (2) so as to each be in communication with it, so that, during the rotation of the test support (1), the sample is, by a centrifugal force, made to flow uniformly into each of the microflow channels (3); a plurality of reaction receptacles (4), which are each arranged on the microflow channels, each of the reaction receptacles (4) having a volume of 20pl to 80pl; a plurality of pre-loaded reagents (41), each of which is loaded into the reaction receptacles (4), each of the pre-loaded reagents (41) being a reagent for an antibiotic susceptibility test, known as "AST"; a plurality of overflow channels (5), each of which is arranged on one side of the reaction receptacles (4), opposite the flow microchannels (3), and which are in communication with them; a plurality of overflow sumps (6), each of which is arranged on one side of the overflow channels (5) and which are in communication with them; a plurality of constriction portions (51), each formed in the overflow channels (5) and having a passage orifice in communication with the reaction receptacles (4), larger than a passage orifice of the latter in communication with the overflow sump (6); and

Claims

a plurality of vent holes (7), each of which is formed on one side of the overflow sumps (6) and is in communication with them.

2. Reagent disc structure for antibiotic susceptibility testing according to claim 1, characterized in that the test support (1) includes at least one fastening part (13) intended to be fixed to a test device (8), and the test support (1) includes a part (14) forming a keying feature, allowing identification of the direction of placement of the test support (1).

3. Reagent disc structure for antibiotic susceptibility testing according to claim 1, characterized in that a convergence groove (42) is provided on one side of each of the reaction receptacles (4) connected to one of the respective overflow channels (5).

4. Reagent disc structure for antibiotic susceptibility testing according to claim 1, characterized in that the flow micro-channels (3) each have a cross-section ranging from 100pm2 to 300pm2.

5. Reagent disc structure for antibiotic susceptibility testing according to claim 1, characterized in that one side of the outer circumference of the central injection part (2) connected to the flow micro-channels (3) is provided with a flow limiting groove (23). 3m / b / n