2-aminopyrimidin-6-ones and analogs exhibiting Anti-cancer and Anti-proliferative activities

Abstract

To provide novel inhibitors that inhibit c-FMS, c-KIT, and / or PDGFR kinases for the treatment of autoimmune diseases which also exhibit selectivity by not potently inhibiting other kinases including ABL, BCR-ABL, KDR, SRC, LCK, LYN, FGFR, MET and other kinases.SOLUTION: The present invention provides a compound of formula I, or a pharmaceutically acceptable salt, an enantiomer, a stereoisomer, or a tautomer thereof.SELECTED DRAWING: None

Description

[Technical field]

[0001] Priority This application claims the benefit of U.S. Provisional Patent Application No. 61 / 792,812, filed March 15, 2013, the entire disclosure of which is incorporated by reference into this application.

[0002] Description of electronically submitted text files The contents of the text files submitted electronically herewith are incorporated herein by reference in their entirety. A computer-readable format copy of the Sequence Listing (Filename: DECP_062_01US_SeqList_ST25.txt, Date of Record: March 15, 2014, File Size: 18 KB).

[0003] Technical Field Compounds are described that find utility in the treatment of cancer, autoimmune diseases, and bone metabolic disorders through inhibition of c-FMS (CSF-1R), c-KIT, and / or PDGFR kinases. These compounds also find utility in the treatment of other mammalian diseases mediated by c-FMS, c-KIT, or PDGFR kinases. [Background technology]

[0004] Background technology Autoimmune diseases, including autoimmune arthritis, represent important human diseases with high morbidity and prevalence. Rheumatoid arthritis affects approximately 0.6% of the world's population (Firestein, GS, Nature (2003) 423:356). Although adaptive immune responses involving the production of autoantibodies that react with tissue antigens are involved in the pathogenesis and early development of these diseases (Edwards, JC et al, New England Journal of Medicine (2004) 350:2572; Genovese, MC et al, New England Journal of Medicine (2005) 353:1114), chronic signs of tissue and joint damage are primarily mediated by cellular events mediated by the innate immune response (Firestein, GS, Nature (2003) 423:356; Paniagua, RT et al, Arthritis Research & Therapy (2010) 12:R32). Contributing cell types from the innate immune response that mediate chronic tissue injury include fibroblast-like synoviocytes, macrophages, mast cells, and osteoclasts.

[0005] Kinases represent a family of proteins that play important roles in mammalian cell functions, including cell proliferation, survival, motility, response to growth factors, and secretion of cytokines and other proinflammatory, proangiogenic, and immunomodulatory substances. Thus, elucidation of the kinases that mediate these events in fibroblast-like synoviocytes, macrophages, mast cells, and osteoclasts represents a reasonable approach to new therapies for the treatment of autoimmune diseases.

[0006] Imatinib is a commercially available kinase inhibitor for the treatment of cancer chronic myeloid leukemia (CML, Druker, BJ et al, New England Journal of Medicine (2001) 344:1031) and gastrointestinal stromal tumors (GIST, Demetri, GD, et al, New England Journal of Medicine (2002) 347:472). Imatinib has also shown benefit in cancer patients coexisting with autoimmune diseases such as rheumatoid arthritis (Ihara, MK et al, Clinical Rheumatology (2003) 22:362; Eklund, KK and Joensuu, H., Ann Medicine (2003) 35:362; Ames, PR et al, Journal of Rheumatology (2008) 35:1682). The kinases inhibited by imatinib that confer its efficacy in the treatment of CML and GIST are BCR-ABL kinase and c-KIT kinase, respectively. In addition to these two kinases, other kinases inhibited by imatinib include c-FMS, PDGFR-α, and PDGFR-β (Dewer, AL et al, Blood (2005) 105:3127; Fabian, MA et al, Nature Biotechnology (2005) 23:329).

[0007] Recent publications have identified that c-FMS kinase is associated with activation of synovial macrophages, PDGFR kinase is associated with activation of fibroblast-like synoviocytes, and c-KIT kinase is associated with activation of mast cells (Paniagua, RT, et al Journal of Clinical Investigation (2006) 116:2633). c-FMS kinase has also been associated with proliferation and differentiation of monocytes into macrophages and osteoclasts, which are recruited to mediate joint damage in rheumatoid arthritis (Paniagua, RT et al, Arthritis Research & Therapy (2010) 12:R32; Yao, Z. et al, Journal of Biological Chemistry (2006) 281:11846; Patel, S. and Player, MR Current Topics in Medicinal Chemistry (2009) 9:599; Pixley, FJ et al, Trends in Cell Biology (2004) 14:628).

[0008] Recently, the importance of the tumor microenvironment in cancer motility, invasion, and metastasis has become more clearly defined. In particular, the role of tumor-associated macrophages (TAMs) in tumor progression has been studied. These host (stromal) macrophages are recruited to the tumor site or premetastatic niche in a way that modifies the tumor environment and makes it more amenable to tumor motility, invasion, and metastasis. These TAMs are known to express the c-FMS receptor tyrosine kinase (also known as CSF-1R) on their surface and to depend on signaling through this kinase by binding to the activating ligands CSF-1 (also known as macrophage colony-stimulating factor, or MCSF) and interleukin-34 (IL-34). Activation of this c-FMS / MCSF (CSF1-R / CSF-1) signaling axis stimulates monocyte proliferation, differentiation into tumor-associated macrophages, and promotes macrophage cell survival. By stimulating TAM components of the tumor microenvironment, activation of c-FMS kinase is associated with tumor cell migration, invasion, and metastasis (J. Condeelis and JW Pollard, Cell (2006) 124:263; S. Patel and MR Player, Current Topics in Medicinal Chemistry (2009) 9:599). Ablation of the ligand of c-FMS kinase, CSF-1, in mice reduced tumor progression and significantly reduced metastasis in a mouse model of breast cancer, whereas overexpression of CSF-1 accelerated metastasis in this model (EY Lin et al, Journal of of Experimental Medicine (2001) 193:727). Furthermore, an interaction between tumor cells and macrophages has been described, where macrophage secretion of the tumor growth factor EGF and tumor cell secretion of CSF-1 establish a paracrine loop that promotes tumor migration and invasion. This paracrine loop was blocked by administration of an antibody against c-FMS kinase (J. Wyckoff et al, Cancer Research (2004) 64:7022). Correlative clinical data also showed that overexpression of CSF-1 in tumors is a predictor of poor prognosis (RD Leek and AL Harris, Journal of Mammary Gland Biology Neoplasia (2002) 7:177; EY Lin et al, Journal of Mammary Gland Biology Neoplasia (2002) 7:147). Activation of c-FMS kinase is also required for osteoclast differentiation and activation. Its involvement in mediating bone metastasis of various cancers, including breast and prostate cancer, has been reported (S. Patel and MR Player, Current Topics in Medicinal Chemistry (2009) 9:599). High plasma concentrations of CSF-1 have been reported in bone metastatic prostate cancer, suggesting activation of prostate cancer bone metastasis (H. Ide, et al, Human Cell (2008) 21:1). c-FMS inhibitors have been reported to reduce radiological bone lesions when evaluated in models of metastatic bone disease (CL Manthey, et al, Molecular Cancer Therapy (2009) 8:3151, H. Ohno et al, Mol. Cancer Therapy (2006) 5:2634). MCSF-mediated activation of both LYVE-1+ and LYVE1- macrophages also mediated pathological angiogenesis and lymphangiogenesis in mouse models of cancer, and blockade of c-FMS signaling resulted in inhibition of tumor angiogenesis / lymphangiogenesis (Y. Kubota et al., Journal of Experimental Medicine (2009) 206:1089).Administration of CSF-1R inhibitors blocked the recruitment of bone marrow-derived TAMs and bone marrow-derived monocytic myeloid-derived suppressor cells (MDSCs) to tumor sites, leading to a marked reduction in tumor angiogenesis and, when combined with anti-VEGFR-2 therapy, synergistically suppressing tumor growth (SJ Princeman, et al. Blood (2010) 115:1461). Irradiation of glioblastoma tumors in mice was shown to cause a transient reduction in tumor size, followed only by a reactive tumor angiogenesis mediated by recruitment of bone marrow-derived monocytes expressing CD11b and F4 / 80 surface antigens (M. Kioi et al, Journal of Clinical Investigation (2010) 120:694). CD11b+ and F4 / 80+ monocytes are also known to express functional c-FMS receptors. Thus, blocking tumor infiltrating c-FMS+ bone marrow-derived monocytes by the use of c-FMS kinase inhibitors offers the possibility of preventing tumor reactive angiogenesis and glioblastoma tumor progression. CSF-1R blockade was also shown to promote the emergence of an antitumor immune program by reversing immune tolerance mechanisms and upregulating CD8+ T cell-mediated tumor suppression in an immunocompetent mouse breast cancer model. Restoration of the antitumor immune program was mechanistically linked to c-FMS inhibitor blockade of TAM-mediated programmed death ligand-1 (PDL-1) immune tolerance (DG DeNardo, et al. Cancer Discovery (2011) 1:OF52). Summary of the Invention [Problem to be solved by the invention]

[0009] Thus, small molecule inhibitors of c-FMS kinase, c-KIT kinase, or PDGFR kinase provide a reasonable approach to new therapies for the treatment of autoimmune diseases, and in particular for blocking chronic tissue destruction mediated by the innate immune system. Inhibition of c-FMS kinase also provides a reasonable approach to new therapies for the treatment of cancer, particularly for the treatment of cancer invasion, cancer angiogenesis or neovasculogenesis, cancer metastasis, cancer immune tolerance, and for the treatment of cancers prone to bone metastasis.

[0010] There is a need to provide kinase inhibitors that selectively inhibit the kinases (c-FMS, c-KIT, PDGFR) that cause chronic tissue destruction in autoimmune diseases without inhibiting other kinases (ABL, BCR-ABL, KDR, SRC, LCK, LYN, FGFR, and other kinases) that are targeted by commercially available cancer therapeutics. The present invention discloses novel inhibitors that inhibit c-FMS, c-KIT, and / or PDGFR kinases for the treatment of autoimmune diseases that also exhibit selectivity by not potently inhibiting other kinases, including ABL, BCR-ABL, KDR, SRC, LCK, LYN, FGFR, MET, and other kinases. The inhibitors of the present invention also find utility in the treatment of other mammalian diseases, including human diseases, that are mediated by c-FMS, c-KIT, or PDGFR kinases. Such diseases include, without limitation, cancer, autoimmune diseases, and bone resorption diseases. [Means for solving the problem]

[0011] Summary of the Invention In one embodiment, a compound of formula I [ka] and pharma- ceutically acceptable salts, enantiomers, stereoisomers, and tautomers thereof are described, During the ceremony, A is selected from the group consisting of -N(R2)R3 and G; G, [ka] and is selected from the group consisting of: ** ) is the point of attachment to the pyrimidine ring; each G moiety may be further substituted with one, two, or three R4 moieties; W is a C5-C6 heteroaryl, phenyl, -NHC(O)R6, -NHC(O)R7, -NHC(O)N(R8)R9, or -C(O)N(R8)R9, each C5-C6 heteroaryl or phenyl optionally substituted with one, two, or three R5 moieties; X1 and X2 are each independently hydrogen or C1-C6 alkyl; R1 is hydrogen, C1-C6 alkyl, deuterated-C1-C6 alkyl in which the alkyl chain is partially or completely deuterated, or branched C3-C8 alkyl; R2 is hydrogen, C1-C6 alkyl, deuterated -C1-C6 alkyl in which the alkyl chain is partially or completely deuterated, branched C3-C8 alkyl, C3-C8 cycloalkyl, fluoro C1-C6 alkyl in which the alkyl is partially or completely fluorinated, -(CH2) m -OR8, or a 3- to 8-membered heterocyclic ring, each alkylene optionally substituted with C1-C4 alkyl; R3 is hydrogen, C1-C6 alkyl, deuterated-C1-C6 alkyl in which the alkyl chain is partially or completely deuterated, branched C3-C8 alkyl, C3-C8 cycloalkyl, fluoro-C1-C6 alkyl in which the alkyl is completely or partially fluorinated, or a 3- to 8-membered heterocyclic ring; Each R4 is independently selected from hydrogen, halogen, C1-C6 alkyl, fluoro-C1-C6 alkyl in which the alkyl chain is partially or completely fluorinated, branched C3-C8 alkyl, C3-C8 cycloalkyl, -(CH2) m -OR8, -(CH2) m -NR8(R9), -(CH2) m -R7, or cyano, each alkylene optionally substituted with C1-C4 alkyl; Each R5 is independently selected from hydrogen, C1-C6 alkyl, deuterated-C1-C6 alkyl in which the alkyl chain is partially or completely deuterated, branched C3-C8 alkyl, halogen, cyano, fluoro-C1-C6 alkyl in which the alkyl chain is partially or completely fluorinated, -(CH2) m -C(O)NR8(R9), -(CH2)m -C(O)R7, -(CH2) m -OR8, -(CH2) m -NR8(R9), or -(CH2) m -R7, each alkylene optionally substituted with C1-C4 alkyl; Each R6 is independently selected from hydrogen, C1-C6 alkyl, branched C3-C8 alkyl, C3-C8 cycloalkyl, -(CH2) m -CN, -(CH2) m -OR8, -(CH2) m -NR8(R9), or -(CH2) m -R7, each alkylene optionally substituted with C1-C4 alkyl; Each R7 is independent and individual. [ka] and the symbol (##) is the point of attachment to the W, R5, or R6 moiety, respectively, containing the R7 moiety; Each R7 is -(R10) p optionally replaced by each R8 and R9 is independently hydrogen, C1-C6 alkyl, fluoro-C1-C6 alkyl in which the alkyl chain is partially or fully fluorinated, or branched C3-C8 alkyl; Each R10 is independently C1-C6 alkyl, -(CH2) m -CN, -(CH2) m -OR3, -(CH2) m -NR8(R9), or -(CH2) m -C(O)-R6, each alkyl or alkylene optionally substituted with one or two C1-C6 alkyl; each alkylene is optionally substituted with C1-C4 alkyl; each m is independently 0, 1, 2, or 3; Each p is 0, 1, 2, or 3.

[0012] In one embodiment of Formula I, A is -N(R2)R3.

[0013] In one embodiment of Formula I, A is G.

[0014] In one embodiment of Formula I, W is a C5-C6 heteroaryl optionally substituted with one, two or three R5.

[0015] In one embodiment of formula I, W is -NHC(O)R6, -NHC(O)R7, or -NHC(O)N(R8)R9.

[0016] In one embodiment of Formula I, W is -NHC(O)R6.

[0017] In one embodiment of Formula I, W is -NHC(O)R7.

[0018] In one embodiment of formula I, W is -NHC(O)N(R8)R9.

[0019] In one embodiment of formula I, W is -C(O)N(R8)R9.

[0020] In one embodiment of formula I, W is phenyl optionally substituted with one, two, or three R5.

[0021] In one embodiment of Formula I, X1 and X2 are each independently hydrogen or C1-C6 alkyl.

[0022] In one embodiment of Formula I, X1 and X2 are hydrogen.

[0023] In one embodiment of Formula I, one of X1 and X2 is hydrogen and the other is C1-C6 alkyl.

[0024] In one embodiment of Formula I, R1 is hydrogen, C1-C6 alkyl, deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, or branched C3-C8 alkyl.

[0025] In one embodiment of Formula I, R1 is hydrogen.

[0026] In one embodiment of Formula I, R1 is C1-C6 alkyl.

[0027] In one embodiment, the compound of formula I is a compound of formula Ia, wherein W is C5-C6 heteroaryl, phenyl, -NHC(O)R6, -NHC(O)R7, -NHC(O)N(R8)R9, or -C(O)N(R8)R9, and each C5-C6 heteroaryl or phenyl is optionally substituted with one, two, or three R5 moieties, or a pharma- ceutically acceptable salt, enantiomer, stereoisomer, or tautomer thereof. [ka]

[0028] In one embodiment of Formula Ia, W is a C5-C6 heteroaryl optionally substituted with one, two or three R5.

[0029] In one embodiment of Formula Ia, W is pyrazolyl, imidazolyl, isoxazolyl, oxazolyl, thiazolyl, triazolyl, or pyridinyl, and each W is optionally substituted with one, two, or three R5.

[0030] In one embodiment of Formula Ia, W is pyrazolyl optionally substituted with one, two or three R5.

[0031] In one embodiment of Formula Ia, W is imidazolyl optionally substituted with one, two or three R5.

[0032] In one embodiment of Formula Ia, W is isoxazolyl optionally substituted with one or two R5.

[0033] In one embodiment of Formula Ia, W is oxazolyl optionally substituted with one or two R5.

[0034] In one embodiment of Formula Ia, W is thiazolyl optionally substituted with one or two R5.

[0035] In one embodiment of Formula Ia, W is triazolyl optionally substituted with one or two R5.

[0036] In one embodiment of Formula Ia, W is pyridinyl optionally substituted with one, two or three R5.

[0037] In one embodiment of formula Ia, W is -NHC(O)R6, -NHC(O)R7, or -NHC(O)N(R8)R9.

[0038] In one embodiment of Formula Ia, W is -NHC(O)R6.

[0039] In one embodiment of Formula Ia, W is -NHC(O)R7.

[0040] In one embodiment of Formula Ia, W is -NHC(O)N(R8)R9.

[0041] In one embodiment of Formula Ia, W is -C(O)N(R8)R9.

[0042] In one embodiment of Formula Ia, W is phenyl optionally substituted with one, two or three R5.

[0043] In one embodiment of Formula Ia, X1 and X2 are each independently hydrogen or C1-C6 alkyl.

[0044] In one embodiment of Formula Ia, X1 and X2 are hydrogen.

[0045] In one embodiment of Formula Ia, one of X1 and X2 is hydrogen and the other is C1-C6 alkyl.

[0046] In one embodiment of Formula Ia, R1 is hydrogen, C1-C6 alkyl, deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, or branched C3-C8 alkyl.

[0047] In one embodiment of Formula Ia, R1 is hydrogen.

[0048] In one embodiment of Formula Ia, R1 is C1-C6 alkyl.

[0049] In one embodiment of formula Ia, R2 is selected from the group consisting of hydrogen, C1-C6 alkyl, deuterated -C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, branched C3-C8 alkyl, C3-C8 cycloalkyl, fluoroC1-C6 alkyl in which the alkyl chain is fully or partially fluorinated, -(CH2) m -OR8, or a 3- to 8-membered heterocyclic ring, each alkylene optionally substituted with C1-C4 alkyl.

[0050] In one embodiment of formula Ia, R2 is C1-C6 alkyl, branched C3-C8 alkyl, C3-C8 cycloalkyl, -(CH2) m -OR8, or a 3- to 8-membered heterocyclic ring, each alkylene optionally substituted with C1-C4 alkyl.

[0051] In one embodiment of Formula Ia, R2 is C1-C6 alkyl.

[0052] In one embodiment of Formula Ia, R2 is a branched C3-C8 alkyl.

[0053] In one embodiment of Formula Ia, R2 is C3-C8 cycloalkyl.

[0054] In one embodiment of Formula Ia, R2 is -(CH2) m-OR8, and each alkylene is optionally substituted with C1-C4 alkyl.

[0055] In one embodiment of Formula Ia, R2 is a 3- to 8-membered heterocyclic ring.

[0056] In one embodiment of Formula Ia, R3 is hydrogen, C1-C6 alkyl, deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, branched C3-C8 alkyl, C3-C8 cycloalkyl, fluoroC1-C6 alkyl in which the alkyl is fully or partially fluorinated, or a 3- to 8-membered heterocyclic ring.

[0057] In one embodiment of Formula Ia, R3 is hydrogen.

[0058] In one embodiment of Formula Ia, R3 is C1-C6 alkyl.

[0059] In one embodiment of Formula Ia, R1 is hydrogen, R2 is C1-C6 alkyl, branched C3-C8 alkyl, or C3-C8 cycloalkyl, and R3 is hydrogen.

[0060] In one embodiment of Formula Ia, R1 is C1-C6 alkyl, R2 is C1-C6 alkyl, branched C3-C8 alkyl, or C3-C8 cycloalkyl, and R3 is hydrogen.

[0061] In one embodiment of Formula Ia, R1 is methyl, R2 is C1-C6 alkyl, branched C3-C8 alkyl or C3-C8 cycloalkyl, and R3 is hydrogen.

[0062] In one embodiment, the compound of formula I is a compound of formula Ib, wherein X1 and X2 are each independently hydrogen or C1-C6 alkyl, or a pharma- ceutically acceptable salt, enantiomer, stereoisomer, or tautomer thereof. [ka]

[0063] In one embodiment of Formula Ib, X1 and X2 are hydrogen.

[0064] In one embodiment of Formula Ib, one of X1 and X2 is hydrogen and the other is C1-C6 alkyl.

[0065] In one embodiment of Formula Ib, R1 is hydrogen, C1-C6 alkyl, deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, or branched C3-C8 alkyl.

[0066] In one embodiment of Formula Ib, R1 is hydrogen.

[0067] In one embodiment of Formula Ib, R1 is C1-C6 alkyl.

[0068] In one embodiment of formula Ib, R2 is selected from the group consisting of hydrogen, C1-C6 alkyl, deuterated -C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, branched C3-C8 alkyl, C3-C8 cycloalkyl, fluoroC1-C6 alkyl in which the alkyl chain is fully or partially fluorinated, -(CH2) m -OR8, or a 3- to 8-membered heterocyclic ring, each alkylene optionally substituted with C1-C4 alkyl.

[0069] In one embodiment of formula Ib, R2 is C1-C6 alkyl, branched C3-C8 alkyl, C3-C8 cycloalkyl, -(CH2) m -OR8, or a 3- to 8-membered heterocyclic ring, each alkylene optionally substituted with C1-C4 alkyl.

[0070] In one embodiment of Formula Ib, R2 is C1-C6 alkyl.

[0071] In one embodiment of Formula Ib, R2 is a branched C3-C8 alkyl.

[0072] In one embodiment of Formula Ib, R2 is C3-C8 cycloalkyl.

[0073] In one embodiment of Formula Ib, R2 is -(CH2) m -OR8, and each alkylene is optionally substituted with C1-C4 alkyl.

[0074] In one embodiment of Formula Ib, R2 is a 3- to 8-membered heterocyclic ring.

[0075] In one embodiment of Formula Ib, R1 is hydrogen and R2 is C1-C6 alkyl, branched C3-C8 alkyl, or C3-C8 cycloalkyl.

[0076] In one embodiment of Formula Ib, R1 is C1-C6 alkyl and R2 is C1-C6 alkyl, branched C3-C8 alkyl, or C3-C8 cycloalkyl.

[0077] In one embodiment of Formula Ib, R1 is methyl and R2 is C1-C6 alkyl, branched C3-C8 alkyl, or C3-C8 cycloalkyl.

[0078] In one embodiment of formula Ib, R5 is selected from the group consisting of hydrogen, C1-C6 alkyl, deuterated -C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, branched C3-C8 alkyl, halogen, cyano, fluoro-C1-C6 alkyl in which the alkyl chain is partially or fully fluorinated, -(CH2) m -C(O)NR8(R9), -(CH2) m -C(O)R7, -(CH2) m -OR8, -(CH2) m -NR8(R9), or -(CH2) m -R7, each alkylene optionally substituted with C1-C4 alkyl.

[0079] In one embodiment of Formula Ib, R5 is hydrogen, C1-C6 alkyl, or deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, or branched C3-C8 alkyl.

[0080] In one embodiment of Formula Ib, R5 is a C1-C6 alkyl or a deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated.

[0081] In one embodiment of Formula Ib, R5 is C1-C6 alkyl.

[0082] In one embodiment, the compound of formula I is a compound of formula Ic, wherein W is C5-C6 heteroaryl, phenyl, -NHC(O)R6, -NHC(O)R7, -NHC(O)N(R8)R9, or -C(O)N(R8)R9, and each C5-C6 heteroaryl or phenyl is optionally substituted with one, two, or three R5 moieties, or a pharma- ceutically acceptable salt, enantiomer, stereoisomer, or tautomer thereof. [ka]

[0083] In one embodiment of Formula Ic, W is a C5-C6 heteroaryl optionally substituted with one, two or three R5.

[0084] In one embodiment of Formula Ic, W is pyrazolyl, imidazolyl, isoxazolyl, oxazolyl, thiazolyl, triazolyl, or pyridinyl, and each W is optionally substituted with one, two, or three R5.

[0085] In one embodiment of Formula Ic, W is pyrazolyl optionally substituted with one, two or three R5.

[0086] In one embodiment of Formula Ic, W is imidazolyl optionally substituted with one, two or three R5.

[0087] In one embodiment of Formula Ic, W is isoxazolyl optionally substituted with one or two R5.

[0088] In one embodiment of Formula Ic, W is oxazolyl optionally substituted with one or two R5.

[0089] In one embodiment of Formula Ic, W is thiazolyl optionally substituted with one or two R5.

[0090] In one embodiment of Formula Ic, W is triazolyl optionally substituted with one or two R5.

[0091] In one embodiment of Formula Ic, W is pyridinyl optionally substituted with one, two, or three R5.

[0092] In one embodiment of formula Ic, W is -NHC(O)R6, -NHC(O)R7, or -NHC(O)N(R8)R9.

[0093] In one embodiment of Formula Ic, W is -NHC(O)R6.

[0094] In one embodiment of Formula Ic, W is -NHC(O)R7.

[0095] In one embodiment of formula Ic, W is -NHC(O)N(R8)R9.

[0096] In one embodiment of formula Ic, W is -C(O)N(R8)R9.

[0097] In one embodiment of Formula Ic, W is phenyl optionally substituted with one, two, or three R5.

[0098] In one embodiment of Formula Ic, X1 and X2 are each independently hydrogen or C1-C6 alkyl.

[0099] In one embodiment of Formula Ic, X1 and X2 are hydrogen.

[0100] In one embodiment of Formula Ic, one of X1 and X2 is hydrogen and the other is C1-C6 alkyl.

[0101] In one embodiment of Formula Ic, R1 is hydrogen, C1-C6 alkyl, deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, or branched C3-C8 alkyl.

[0102] In one embodiment of Formula Ic, R1 is hydrogen.

[0103] In one embodiment of Formula Ic, R1 is C1-C6 alkyl.

[0104] In one embodiment of formula Ic, G is [ka] and is selected from the group consisting of: ** ) is the point of attachment to the pyrimidine ring, Each G moiety may be further substituted with one, two, or three R4 moieties.

[0105] In one embodiment of Formula Ic, R1 is hydrogen and G is [ka] and is selected from the group consisting of: ** ) is the point of attachment to the pyrimidine ring, Each G moiety may be further substituted with one, two, or three R4 moieties.

[0106] In one embodiment of Formula Ic, R1 is C1-C6 alkyl and G is [ka] and is selected from the group consisting of: ** ) is the point of attachment to the pyrimidine ring, Each G moiety may be further substituted with one, two, or three R4 moieties.

[0107] In one embodiment of Formula Ic, R1 is methyl and G is [ka] and is selected from the group consisting of: ** ) is the point of attachment to the pyrimidine ring, Each G moiety may be further substituted with one, two, or three R4 moieties.

[0108] In one embodiment, the compound of formula I is a compound of formula Id, wherein X1 and X2 are each independently hydrogen or C1-C6 alkyl, or a pharma- ceutically acceptable salt, enantiomer, stereoisomer, or tautomer thereof. [ka]

[0109] In one embodiment, the compound of formula Id is a compound wherein X1 and X2 are hydrogen.

[0110] In one embodiment, the compound of formula Id is one where one of X1 and X2 is hydrogen and the other is C1-C6 alkyl.

[0111] In one embodiment, the compound of formula Id is a compound wherein R1 is hydrogen, C1-C6 alkyl, deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, or branched C3-C8 alkyl.

[0112] In one embodiment, the compound of formula Id is a compound wherein R1 is hydrogen.

[0113] In one embodiment, the compound of formula Id is a compound wherein R1 is C1-C6 alkyl.

[0114] In one embodiment, the compound of formula Id is wherein G is [ka] and is selected from the group consisting of: ** ) is the point of attachment to the pyrimidine ring; Compounds in which each G moiety may be further substituted with one, two, or three R4 moieties.

[0115] In one embodiment, the compound of formula Id is a compound of formula Id, wherein R1 is hydrogen and G is [ka] and is selected from the group consisting of: ** ) is the point of attachment to the pyrimidine ring; Compounds in which each G moiety may be further substituted with one, two, or three R4 moieties.

[0116] In one embodiment, the compound of formula Id is a compound of formula Id, wherein R1 is C1-C6 alkyl and G is [ka] and is selected from the group consisting of: ** ) is the point of attachment to the pyrimidine ring; Compounds in which each G moiety may be further substituted with one, two, or three R4 moieties.

[0117] In one embodiment, the compound of formula Id is a compound of formula Id, wherein R1 is methyl and G is [ka] and is selected from the group consisting of: ** ) is the point of attachment to the pyrimidine ring; Compounds in which each G moiety may be further substituted with one, two, or three R4 moieties.

[0118] In one embodiment, the compound of formula Id is a compound wherein R5 is hydrogen, C1-C6 alkyl, deuterated -C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, branched C3-C8 alkyl, halogen, cyano, fluoro-C1-C6 alkyl in which the alkyl chain is partially or fully fluorinated, -(CH2)mC(O)NR8(R9), -(CH2)mC(O)R7, -(CH2)m-OR8, -(CH2)m-NR8(R9), or -(CH2)m-R7, each alkylene optionally substituted with C1-C4 alkyl.

[0119] In one embodiment, the compound of formula Id is a compound wherein R5 is hydrogen, C1-C6 alkyl, or deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, or branched C3-C8 alkyl.

[0120] In one embodiment, the compound of formula Id is a compound wherein R5 is a C1-C6 alkyl or a deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated.

[0121] In one embodiment, the compound of formula Id is a compound wherein R5 is C1-C6 alkyl.

[0122] In some embodiments, the present invention provides a method for the preparation of a compound according to the present invention, comprising administering to a patient a therapeutically effective amount of 2-(ethylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(dimethylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(isopropylamino)-5-(6-methyl-5-((2-(1-methyl-1H -pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(ethylamino)-5-(6-methyl-5-((6'-methyl-[2,3'-bipyridine]-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(ethylamino)-5-(6-methyl-5-((2-(4-methyl-1H-imidazol-1-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-((2-methoxyethyl) Amino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(methylamino)pyrimidin-4(3H)-one, 2-(ethylamino)-5-(5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl ) pyrimidin-4(3H)-one, 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidin-4(3H)-one, 2-(isopropylamino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 4-((6-(2-(isopropylamino)-6-oxo-1,6-dihydropyrimidin-5-yl)pyridin-3-yl)oxy)-N-methylpicolinamide, 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-morpholinopyrimidin-4(3H)-one, 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(piperidin-1-yl)pyrimidin-4(3H)-one, 2-(cyclopropylamido) 2-(cyclopentylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(cyclopentylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2 -yl)-2-(pyrrolidin-1-yl)pyrimidin-4(3H)-one, 2-(cyclopropylamino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(isopropylamino)-5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, N-(4-((6-(2 -(isopropylamino)-6-oxo-1,6-dihydropyrimidin-5-yl)-2-methylpyridin-3-yl)oxy)pyridin-2-yl)acetamide, 5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidin-4(3H)-one, 5-(5-((2-(1-ethyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)-6-methylpyridin-2-yl)-2-(, isopropylamino)pyrimidin-4(3H)-one, (R)-2-((1-methoxypropan-2-yl)amino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, (R)-2-(2-(methoxymethyl)pyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one , (S)-2-(3-(dimethylamino)pyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(ethylamino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-((2-methoxyethyl)amino)-3-methyl-5-(6-methyl-5 -((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-((tetrahydro-2H-pyran-4-yl)amino)pyrimidin-4(3H)-one, 2-(tert-butylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-pyrimidin-4(3H)-one )pyridin-2-yl)pyrimidin-4(3H)-one, 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(neopentylamino)pyrimidin-4(3H)-one, and 2-(3,3-difluoropyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one.

[0123] In certain embodiments, the invention includes a method for treating a mammalian disease mediated at least in part by the kinase activity of c-FMS, PDGFR-β, or c-KIT kinase, wherein the kinase is in a wild-type form, a mutated oncogenic form, an aberrant fusion protein form, or a polymorph thereof, the method comprising administering to a mammal in need thereof an effective amount of a compound of formula I.

[0124] In another embodiment, the present invention comprises a pharmaceutical composition comprising a compound of formula I and a pharma- ceutically acceptable carrier.

[0125] In certain embodiments, the composition comprises an additive selected from an adjuvant, excipient, diluent, or stabilizer.

[0126] In some embodiments, the present invention includes a method of treating cancer, gastrointestinal stromal tumors, hyperproliferative diseases, metabolic diseases, neurodegenerative diseases, solid tumors, melanoma, glioblastoma, ovarian cancer, pancreatic cancer, prostate cancer, lung cancer, breast cancer, kidney cancer, liver cancer, osteosarcoma, multiple myeloma, cervical cancer, metastasis of a primary tumor site, cancer metastatic to bone, papillary thyroid cancer, non-small cell lung cancer, colorectal cancer, rheumatoid arthritis, osteoarthritis, multiple sclerosis, autoimmune nephritis, lupus, Crohn's disease, asthma, chronic obstructive pulmonary disease, osteoporosis, mastocytosis, or mast cell leukemia, comprising administering to a patient in need thereof an effective amount of a compound of formula I.

[0127] In some embodiments, the present invention includes a method for treating glioblastoma, breast cancer, pancreatic cancer, metastasis of a primary tumor site, or cancer metastatic to bone, comprising administering to a patient in need thereof an effective amount of a compound of formula 1.

[0128] In certain embodiments of the method, the compound is administered orally, parenterally, by inhalation, or subcutaneously.

[0129] In some embodiments, the present invention provides a use of a compound of formula I, or a pharmacologic acceptable salt thereof, in the treatment of cancer, gastrointestinal stromal tumors, hyperproliferative diseases, metabolic diseases, neurodegenerative diseases, solid tumors, melanoma, glioblastoma, ovarian cancer, pancreatic cancer, prostate cancer, lung cancer, breast cancer, kidney cancer, liver cancer, osteosarcoma, multiple myeloma, cervical cancer, metastasis of a primary tumor site, cancer metastatic to bone, papillary thyroid cancer, non-small cell lung cancer, colorectal cancer, rheumatoid arthritis, osteoarthritis, multiple sclerosis, autoimmune nephritis, lupus, Crohn's disease, asthma, chronic obstructive pulmonary disease, osteoporosis, mastocytosis, or mast cell leukemia, the method comprising administering to a patient in need thereof an effective amount of a compound of formula I.

[0130] In some embodiments, the present invention provides a use of a compound of formula I, or a pharma- ceutically acceptable salt thereof, in the treatment of glioblastoma, breast cancer, pancreatic cancer, metastasis of a primary tumor site, or cancer metastatic to bone, the method comprising administering to a patient in need thereof an effective amount of a compound of formula 1.

[0131] In some embodiments, the invention provides the use of a compound of formula I, or a pharma- ceutically acceptable salt thereof, for the manufacture of a medicament for the treatment of cancer, gastrointestinal stromal tumors, hyperproliferative diseases, metabolic diseases, neurodegenerative diseases, solid tumors, melanoma, glioblastoma, ovarian cancer, pancreatic cancer, prostate cancer, lung cancer, breast cancer, renal cancer, liver cancer, osteosarcoma, multiple myeloma, cervical cancer, metastasis of a primary tumor site, cancer metastatic to bone, papillary thyroid cancer, non-small cell lung cancer, colorectal cancer, rheumatoid arthritis, osteoarthritis, multiple sclerosis, autoimmune nephritis, lupus, Crohn's disease, asthma, chronic obstructive pulmonary disease, osteoporosis, mastocytosis, or mast cell leukemia.

[0132] In certain embodiments, the present invention provides the use of a compound of formula 1, or a pharma- ceutically acceptable salt thereof, for the manufacture of a medicament for the treatment of glioblastoma, breast cancer, pancreatic cancer, metastasis of a primary tumor site, or cancer metastatic to bone.

[0133] Details of the present invention are described in the accompanying description below. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, exemplary methods and materials are described herein. Other features, objects, and advantages of the present invention will become apparent from the description and claims. In this specification and the appended claims, the singular form includes the plural form unless the context clearly dictates otherwise. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0134] Throughout this disclosure, various patents, patent applications, and publications are referenced. The disclosures of these patents, patent applications, and publications in their entireties are incorporated by reference into this disclosure to more fully describe the state of the art as known to those skilled in the art as of the date of this disclosure. In the event of any inconsistency between the patents, patent applications, and publications and this disclosure, the present disclosure will control.

[0135] For convenience, certain terms used in the specification, examples, and claims are collected here. Unless otherwise defined, all technical and scientific terms used in this disclosure have the same meaning as commonly understood by those skilled in the art to which this invention belongs. The initial definition provided for a group or term provided in this disclosure applies to that group or term individually or as part of another group throughout this disclosure, unless otherwise indicated.

[0136] The compounds of the present disclosure include any and all possible isomers, stereoisomers, enantiomers, diastereomers, tautomers, and pharma- ceutically acceptable salts. Thus, as used in this disclosure, the terms "compound," "compounds," "test compound," or "test compounds" refer to the compounds of the present disclosure, as well as any and all possible isomers, stereoisomers, enantiomers, diastereomers, tautomers, and pharma- ceutically acceptable salts thereof. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0137] definition The term "alkyl" as used herein refers to a straight chain alkyl, with the length of the alkyl chain being designated by a range of numbers. In exemplary embodiments, "alkyl" refers to an alkyl chain as defined above containing 1, 2, 3, 4, 5, or 6 carbons (i.e., C1-C6 alkyl). Examples of alkyl groups include, but are not limited to, methyl, ethyl, propyl, butyl, pentyl, and hexyl.

[0138] The term "branched alkyl" as used herein refers to an alkyl chain where there is a branch point within the chain, and the total number of carbons in the chain is designated by a range of numbers. In an exemplary embodiment, "branched alkyl" refers to an alkyl chain as defined above that contains 3, 4, 5, 6, 7, or 8 carbons (i.e., a branched C3-C8 alkyl). Examples of branched alkyl groups include, but are not limited to, isopropyl, isobutyl, secondary and tertiary butyl, 2-pentyl, 3-pentyl, 2-hexyl, and 3-hexyl.

[0139] The term "alkoxy" as used herein refers to --O-(alkyl), where "alkyl" is defined above.

[0140] The term "branched alkoxy" as used herein refers to -O-(branched alkyl), where "branched alkyl" is as defined above.

[0141] The term "alkylene" as used herein refers to an alkyl moiety that is interposed between two other atoms. In an exemplary embodiment, "alkylene" refers to an alkyl moiety as defined above that contains 1, 2, or 3 carbons. Examples of alkylene groups include, but are not limited to, -CH2-, -CH2CH2-, and -CH2CH2CH2-. In an exemplary embodiment, the alkylene group is branched.

[0142] The term "alkynyl" as used herein refers to a carbon chain containing one carbon-carbon triple bond. In an exemplary embodiment, "alkynyl" refers to a carbon chain as defined above containing two or three carbons (i.e., C2-C3 alkynyl). Examples of alkynyl groups include, but are not limited to, ethyne and propyne.

[0143] The term "aryl" as used herein refers to a cyclic hydrocarbon, where the ring is characterized by delocalized π electrons (aromaticity) shared between the ring members, and the number of ring atoms is indicated by a range of numbers. In an exemplary embodiment, "aryl" refers to a cyclic hydrocarbon as defined above containing 6, 7, 8, 9, or 10 ring atoms (i.e., C6-C10 aryl). Examples of aryl groups include, but are not limited to, benzene, naphthalene, tetralin, indene, and indane.

[0144] The term "cycloalkyl" as used herein refers to a monocyclic saturated carbocyclic ring, where the number of ring atoms is designated by a range of numbers. In an exemplary embodiment, "cycloalkyl" refers to a carbocyclic ring as defined above containing 3, 4, 5, 6, 7, or 8 ring atoms (i.e., C3-C8 cycloalkyl). Examples of cycloalkyl groups include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl.

[0145] The term "halogen" or "halo" as used herein refers to fluorine, chlorine, bromine, and iodine.

[0146] The term "heterocycle" or "heterocyclyl" as used herein refers to a cyclic hydrocarbon, at least one of the ring atoms is O, N, or S, and the number of ring atoms is indicated by a range of numbers. A heterocyclyl moiety as defined herein has a C or N bond through which the heterocyclyl ring is connected to an adjacent moiety. For example, in some embodiments, the ring N atom from the heterocyclyl is the attachment atom of the heterocyclic moiety. In exemplary embodiments, "heterocyclyl" refers to a monocyclic hydrocarbon containing 4, 5, 6, 7, or 8 ring atoms (i.e., C4-C8 heterocyclyl). Examples of heterocyclic groups include, but are not limited to, aziridine, oxirane, thiirane, azetidine, oxetane, thietane, pyrrolidine, tetrahydrofuran, pyran, thiopyran, thiomorpholine, thiomorpholine S-oxide, thiomorpholine S-dioxide, oxazoline, tetrahydrothiophene, piperidine, tetrahydropyran, thiane, imidazolidine, oxazolidine, thiazolidine, dioxolane, dithiolane, piperazine, oxazine, dithiane, and dioxane.

[0147] The term "heteroaryl" as used herein refers to a cyclic hydrocarbon, where at least one of the ring atoms is O, N, or S, the ring is characterized by delocalized π electrons (aromaticity) shared between the ring members, and the number of ring atoms is indicated by a range of numbers. Heteroaryl moieties as defined herein have a C or N bond through which the heteroaryl ring is connected to an adjacent moiety. For example, in some embodiments, the ring N atom from the heteroaryl is the bonding atom of the heteroaryl moiety. In exemplary embodiments, "heteroaryl" refers to a cyclic hydrocarbon as defined above containing 5 or 6 ring atoms (i.e., C5-C6 heteroaryl). Examples of heteroaryl groups include, but are not limited to, pyrrole, furan, thienes, oxazole, thiazole, isoxazole, isothiazole, imidazole, pyrazole, oxadiazole, thiadiazole, triazole, tetrazole, pyridine, pyrimidine, pyrazine, pyridazine, and triazine.

[0148] As used herein, the term "substituted" in connection with a moiety refers to a further substituent bonded to the moiety at any permitted location of the moiety. Unless otherwise indicated, the moiety may be bonded through a carbon, nitrogen, oxygen, sulfur, or any other permitted atom.

[0149] The term "salt" as used herein includes pharma- ceutically acceptable salts commonly used to form alkali metal salts of free acids and to form addition salts of free bases. The nature of the salt is not critical, provided that it is pharma- ceutically acceptable. Suitable pharma- ceutically acceptable acid addition salts can be prepared from inorganic acids or from organic acids. Exemplary pharmaceutical salts are disclosed in Stahl, PH, Wermuth, CG, Eds. Handbook of Pharmaceutical Salts: Properties, Selection and Use; Verlag Helvetica Chimica Acta / Wiley-VCH: Zurich, 2002, the contents of which are incorporated herein by reference in their entirety. Specific non-limiting examples of inorganic acids are hydrochloric acid, hydrobromic acid, hydroiodic acid, nitric acid, carboxylic acid, sulfuric acid, and phosphoric acid. Suitable organic acids include, without limitation, aliphatic, cycloaliphatic, aromatic, arylaliphatic, and heterocyclyl-containing carboxylic and sulfonic acids, such as formic acid, acetic acid, propionic acid, succinic acid, glycolic acid, gluconic acid, lactic acid, malic acid, tartaric acid, citric acid, ascorbic acid, glucuronic acid, maleic acid, fumaric acid, pyruvic acid, aspartic acid, glutamic acid, benzoic acid, anthranilic acid, mesylic acid, stearic acid, salicylic acid, p-hydroxybenzoic acid, phenylacetic acid, mandelic acid, embonic acid (pamoic acid), methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, pantothenic acid, toluenesulfonic acid, 2-hydroxyethanesulfonic acid, sulfanilic acid, cyclohexylaminosulfonic acid, algenic acid, 3-hydroxybutyric acid, galactaric acid, or galacturonic acid. Suitable pharma- ceutically acceptable salts of the free acid-containing compounds disclosed herein include, without limitation, metal salts and organic salts.Exemplary metal salts include, but are not limited to, suitable alkali metal (group Ia) salts, alkaline earth metal (group IIa) salts, and other physiologically acceptable metal salts.Such salts can be made from aluminum, calcium, lithium, magnesium, potassium, sodium, and zinc.Exemplary organic salts can be made from primary amines, secondary amines, tertiary amines, and quaternary ammonium salts, such as tromethamine, diethylamine, tetra-N-methylammonium, N,N'-dibenzylethylenediamine, chloroprocaine, choline, diethanolamine, ethylenediamine, meglumine (N-methylglucamine), and procaine.

[0150] As used herein, the terms "administer," "administering," or "administration" refer to the direct administration of either a compound, or a pharma- ceutically acceptable salt of the compound, or a composition to a subject.

[0151] The term "carrier" as used herein encompasses carriers, excipients, and diluents and means a material, composition, or vehicle, such as a liquid or solid filler, diluent, excipient, solvent, or sealing material, that is involved in carrying or transporting a drug from one organ or part of the body to another organ or part of the body.

[0152] The term "disorder" is used in this disclosure to mean, and is used interchangeably with, the terms disease, condition, or illness, unless otherwise indicated.

[0153] The terms "effective amount" and "therapeutically effective amount" are used interchangeably in this disclosure and refer to the amount of a compound that can reduce the symptoms of a disorder in a subject when administered to the subject. The actual amount, including "effective amount" or "therapeutically effective amount", varies depending on many conditions, including but not limited to the specific disorder being treated, the severity of the disorder, the size and health of the patient, and the route of administration. A skilled physician can easily determine the appropriate amount using methods well known in the medical field.

[0154] As used herein, the terms "isolated" and "purified" refer to a component separated from other components of a reaction mixture or natural source. In certain embodiments, an isolate contains at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, or at least about 98% by weight of the compound, or a pharma- ceutically acceptable salt of the compound, of the isolate.

[0155] As used herein, the phrase "pharmacologically acceptable" refers to compounds, materials, compositions and / or dosage forms that are suitable for use in contact with the tissues of human beings and animals without undue toxicity, irritation, allergic response, or other problem or complication, within the scope of safe medical judgment, and commensurate with a reasonable benefit / risk ratio.

[0156] As used in this disclosure, the term "patient" or "subject" includes, without limitation, a human or an animal. Exemplary animals include, but are not limited to, mammals such as mice, rats, guinea pigs, dogs, cats, horses, cows, pigs, monkeys, chimpanzees, baboons, or rhesus monkeys.

[0157] The terms "treatment," "treat," and "treating" are intended to include the full range of interventions for cancer suffered by a patient, such as administration of active compounds to improve, slow, or reverse one or more of the symptoms, and to slow the progression of the cancer even if the cancer is not actually eliminated. Treatment may be to cure, ameliorate, or at least partially alleviate the disorder.

[0158] Structural, chemical, and stereochemical definitions are broadly taken from the IUPAC recommendations, more specifically from the Terminology used in Physical Organic Chemistry compiled by Muller, P. Pure Appl. Chem. 1994, 66, pp. 1077-1184 (IUPAC Recommendations 1994) and Basic Terminology in Stereochemistry compiled by Moss, G. Pure Appl. Chem. 1996, 68, pp. 2193-2222 (IUPAC Recommendations 1996).

[0159] Atropisomers can be isolated as separate chemical species and are defined as a subclass of conformational isomers resulting from restricted rotation about a single bond.

[0160] Positional or structural isomers are defined as isomers that involve the same atoms in different arrangements.

[0161] An enantiomer is defined as one of a pair of molecular entities that are mirror images of each other and are non-superimposable.

[0162] Diastereomers or diastereoisomers are defined as stereoisomers other than enantiomers. Diastereomers or diastereoisomers are stereoisomers that are not related to mirror images. Diastereoisomers are characterized by differences in physical properties and some differences in chemical behavior towards achiral and chiral reagents.

[0163] As used herein, the term "tautomer" refers to a compound produced by the phenomenon of the transfer of a proton from one atom of a molecule to another atom. See March, Advanced Organic Chemistry: Reactions, Mechanisms and Structures, 4th Ed., John Wiley & Sons, pp. 69-74 (1992). Tautomerism is a process that can be used to describe the general formula

number

[0164] The exemplified compounds of the present invention are preferably formulated as pharmaceutical compositions using pharma- ceutically acceptable carriers and administered by various routes. Preferably, such compositions are for oral administration. Such pharmaceutical compositions and processes for their preparation are well known in the art. For example, see REMINGTON: THE SCIENCE AND PRACTICE OF PHARMACY (A. Gennaro, et al., eds., 1999). th ed., Mack Publishing Co., 1995).

[0165] The compounds of formula (I), or pharma- ceutically acceptable salts thereof, can be prepared by a variety of procedures well known in the art, as well as those described below. Certain synthetic steps can be combined in different ways to prepare the compounds of formula I, or pharma- ceutically acceptable salts thereof.

[0166] The compounds used as initial starting materials in the synthesis of compounds of formula Ia are well known and, to the extent not commercially available, are readily synthesized by standard procedures commonly employed by those of skill in the art using the specific criteria provided or can be found in general reference books.

[0167] Examples of well-known procedures and methods include Comprehensive Organic Transformations, VCH Publishers Inc, 1989; Compendium of Organic Synthetic Methods, Volumes 1-10, 1974-2002, Wiley Interscience; Advanced Organic Chemistry, Reactions Mechanisms, and Structure, 5 th Edition, Michael B. Smith and Jerry March, Wiley Interscience, 2001, Advanced Organic Chemistry, 4 th Edition, Part B, Reactions and Synthesis, Francis A. Carey and Richard J. Sundberg, Kluwer Academic / Plenum Publishers, 2000, as well as those described in the references cited therein.

[0168] ChemDraw version 10 or 12 (CambridgeSoft Corporation, Cambridge, Mass.) was used to draw structures of intermediates and example compounds.

[0169] The following abbreviations are used in this disclosure and have the following definitions: "ADP" is adenosine diphosphate, "conc." is concentrated, "DBU" is 1,8-diazabicyclo[5.4.0]undec-7-ene, "DCE" is 1,2-dichloroethane, "DCM" is dichloromethane, "DIEA" is N,N-diisopropylethylamine, "DMA" is N,N-dimethylacetamide, "DMAP" is 4-(dimethylamino)pyridine, "DMF" is N,N-dimethylformamide, and "dppf" is 1,1 ′-bis(diphenylphosphino)ferrocene, “DMEM” is Dulbecco's modified Eagle's medium, “DMSO” is dimethyl sulfoxide, “DPPA” is diphenylphosphuryl azide, “ESI” is electrospray ionization, “Et2O” is diethyl ether, “EtOAc” is ethyl acetate, “EtOH” is ethanol, “GST” is glutathione S-transferase, “h” is hour(s), “Hex” is hexane, and “IC 50" is the half maximal inhibitory concentration, "LiMHDS" is lithium bis(trimethylsilyl)amide, "mCPBA" is 3-chloroperbenzoic acid, "MeCN" is acetonitrile, "MeOH" is methanol, "Me4tBuXPhos" is di-tert-butyl(2',4',6'-triisopropyl-3,4,5,6-tetramethyl-[1,1'-biphenyl]-2-yl)phosphine, "MHz" is megahertz, "min" is minute(s), "MS" is mass spectrometry, "MTBE" is methyl tert-butyl ether, "NADH" is nicotinamide adenine dinucleotide, "NBS" is N-bromosuccinimide, "NMR" is nuclear magnetic resonance, "PBS" is phosphate buffered saline, and "Pd / C" is palladium on carbon. , "Pd2(dba)3" is tris(dibenzylideneacetone)dipalladium(0), "Pd(PPh3)4" is tetrakis(triphenylphosphine)palladium(0), "Prep-HPLC" is preparative high performance liquid chromatography, "RT" is room temperature also known as "ambient temperature" which is understood to comprise the range of normal laboratory temperatures ranging from 15 to 25°C, "satd." is saturated, "TEA" is triethylamine, "TFA" is trifluoroacetic acid, "THF" is tetrahydrofuran, "Tris" is tris(hydroxymethyl)aminomethane, "Xantphos" is 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene, and "X-Phos" is 2-dicyclohexylphosphino-2',4',6'-triisopropylbiphenyl. general chemistry

[0170] Compounds of formula I are prepared by the general synthetic methods shown in the following schemes and accompanying examples. Suitable reaction conditions for the steps of these schemes are well known in the art, and suitable substitutions of solvents and co-reagents are within the skill of the art. Those skilled in the art will appreciate that synthetic intermediates can be isolated and / or purified by well-known techniques as necessary or desired, and that various intermediates can be used directly in subsequent synthetic steps with little or no purification. Furthermore, those skilled in the art will appreciate that in some cases, the order in which moieties are introduced is not critical. The specific order of steps required to produce compounds of formula I will depend on the particular compound being synthesized, the starting compound, and the relative lability of the substituting moieties, as is well understood by a chemist of ordinary skill. Unless otherwise indicated, all substitutes are as defined above.

[0171] Compounds of formula I may contain -NH or -OH moieties at positions W and A. It will be appreciated by those skilled in the art that in some cases it may be advantageous to temporarily mask one or more -NH or -OH moieties using amine protecting groups during synthesis. The protecting groups may be removed from any subsequent intermediate in the synthetic sequence using standard conditions that result in the removal of the protecting groups, which conditions are well known to those skilled in the art. If not specified in the scheme, it will be appreciated by those skilled in the art that the W and A moieties depicted in the scheme below may optionally contain standard amino or hydroxyl protecting groups that may be removed at any time during the synthetic sequence.

[0172] Compounds 1 of the invention (formula I, where R1 is H) can be prepared as shown in scheme 1. In one embodiment, dipyridine 2 can be reacted with boronate 3 or boronic acid 4 to provide intermediate 5. The reaction of 2 with 3 or 4 is generally carried out with heating in the presence of a palladium catalyst, such as Pd(PPh3)4, and a base, such as potassium carbonate. Further conversion of 5 to 7 occurs by reaction of 5 with a reagent MW (6), where M is a trialkylstannyl or boronic acid or boronic ester (when W is heteroaryl), or M is H (when W is -NHC(O)R6, -NHC(O)R7, -NHC(O)N(R8)R9). Conditions for the conversion of 5 to 7 depend on the nature of the W moiety, but generally involve the use of a palladium catalyst, such as Pd(PPh3)4 or Pd2(dba)3, optionally in the presence of an additional ligand, such as xanthophos. General conditions for achieving these transformations are well known to those skilled in the art and are further illustrated in the accompanying Examples. Finally, cleavage of the methyl ether of 7, for example by treatment with HBr or trimethylsilyl iodide, provides compounds of formula 1 where R1 is H.

[0173] In another embodiment of Scheme 1, compound 7 can be prepared directly from 8 (Z is bromine or iodine) by reaction with 3 or 4.

[0174] In another embodiment of Scheme 1, intermediate 7 can be prepared from 2 by a sequence beginning with the reaction of 2 with 9 or 10 to provide 11. Reaction of 11 with MW (6) as above provides 12. In another embodiment, reaction of 8 with 9 or 10 gives 12 directly. Reaction of 12 with an amine A-H13 provides 7. In one embodiment, conversion of 12 to 7 is achieved by sequential treatment with an oxidizing agent such as m-chloroperoxybenzoic acid, resulting in oxidation of the thiomethyl moiety of 12 to a methylsulfoxide or methylsulfone intermediate, followed by treatment of the intermediate with A-H13. [ka]

[0175] Scheme 2 illustrates the preparation of compounds of formula 18 (Formula I, where R1 is alkyl or branched alkyl). In a similar manner to Scheme 1, compound 2 can be reacted with 14 or 15 and a palladium catalyst to give 16. Further reaction of 16 above with MW (6) provides 17. Intermediate 17 can also be prepared by reaction of 8 (Z is bromine or iodine) with 14 or 15 and a palladium catalyst. Reaction of 17 with amine AH (13) gives 18. In one embodiment, the thiomethyl moiety of 17 is oxidized to methyl sulfoxide or methyl sulfone prior to treatment with amine A-H13. [ka]

[0176] General intermediate 2 is prepared as shown in Scheme 3. Treatment of hydroxypyridine 19 with iodine in the presence of a carbonate base gives iodide 20. Further treatment of 20 with 2,4-dichloropyridine (21) in the presence of a base, such as potassium carbonate, provides 2. [ka] Scheme 3

[0177] General intermediate 8 is prepared as shown in Scheme 4. Thus, intermediate 22 (Y is a halogen) is reacted with 2-chloro-4-hydroxypyridine (23) in the presence of a base, e.g., potassium carbonate, to provide nitro ether 24. Reduction of the nitro moiety of 24 provides amine 25. Conditions for the conversion of 24 to 25 are well known to those skilled in the art and include the use of zinc powder in the presence of ammonium chloride in a protic solvent such as methanol. Further reaction of 25 with MW (6) as above provides 26. In one embodiment, the order of steps for the conversion of 24 to 26 is reversed such that 24 is reacted with M-W6 first. The product of the reaction containing the nitro moiety is then reduced to provide 26. Conversion of amine 26 to 8 is achieved by conversion of the amino moiety of 26 to a diazonium salt and in situ replacement of the diazonium moiety with a halogen. Conditions which effect the conversion of 26 to 8 (Z is bromine) include treatment of a mixture of 26 and tetrabutylammonium bromide in dibromomethane with tert-butyl nitrite. Conditions which effect the conversion of 26 to 8 (Z is iodine) include treatment of a mixture of 26 and potassium iodide in diiodomethane with tert-butyl nitrite. [ka]

[0178] Scheme 5 shows the synthesis of boronic esters / boronic acids 3 / 4, 9 / 10, and 14 / 15. In one embodiment of Scheme 5, chloro-pyrimidine 27 reacts with amine A-H13 to give 28. Conditions for the conversion include combining 27 and 13 in a solvent such as THF and heating the mixture to effect the reaction. Further reaction of 28 with bis(pinacol)diboron (34) by heating in the presence of a palladium catalyst such as PdCl2(dppf) and a weak base such as potassium acetate provides the sensitive boronate 3 and / or boronic acid 4. In fact, it is not necessary to separate the possible mixture of 3 and 4 for further use, and the product(s) of the reaction of 28 and 34 are usually used in crude form without further purification. In another embodiment, 29 and 34 react under the same conditions to give 9 and / or 10. Indeed, the product(s) of the reactions of 29 and 34 may be used in crude form without further purification. [ka]

[0179] In another embodiment of Scheme 5, pyrimidinone 30 can be alkylated with R1-X (31, where X is a halogen) to provide 32. In one embodiment, the alkylation occurs by treatment of 29 in a solvent such as THF or DMF with a strong base, for example, lithium bis(trimethylsilyl)amide, followed by addition of 31. Bromination of 32 provides 33. In one embodiment, the order of steps for the conversion of 30 to 33 is reversed such that 30 is brominated prior to alkylation with R1-X 31. Finally, reaction of 33 with 34 provides boronate 14 and / or boronic acid 15. Indeed, the product(s) of the reaction of 33 and 34 can be used in crude form without further purification.

[0180] General intermediate 38, Example 8, where W is -C(O)N(R8)R9, is prepared as shown in Scheme 6. Thus, hydroxypyridine 35 is reacted with chloropicolinamide 36 in the presence of a base, such as potassium tert-butoxide, to provide ether 37. Diazotization of the amino moiety of 37 in the presence of iodide gives iodopyridine 38. [ka]

[0181] The following compounds were made using the synthetic procedures and methods described herein, as well as methods well known to one of ordinary skill in the art: 2-(ethylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(dimethylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(isopropylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, )pyridin-2-yl)pyrimidin-4(3H)-one, 2-(ethylamino)-5-(6-methyl-5-((6'-methyl-[2,3'-bipyridin]-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(ethylamino)-5-(6-methyl-5-((2-(4-methyl-1H-imidazol-1-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-((2-methoxyethyl)amino)-5-(6-methyl-5-((2-(1-methyl-1H-pyridinyl) 2-(ethylamino)-5-(5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(ethylamino)-5-(5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 5-(6-methyl-5-((2-(1-methyl- 1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidin-4(3H)-one, 2-(isopropylamino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 4-((6-(2-(isopropylamino)-6-oxo-1,6-dihydropyrimidin-5-yl)pyridin-3-yl)oxy)-N-methylpicolinamide,5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-morpholinopyrimidin-4(3H)-one, 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(piperidin-1-yl)pyrimidin-4(3H)-one, 2-(cyclopropylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl) pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(cyclopentylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidin-4(3H)-one, 2-(cyclopropylamino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(isopropylamino)-5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, N-(4-((6-(2-(isopropylamino)-6-oxo-1,6-dihydropyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one 5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidin-4(3H)-one, 5-(5-((2-(1-ethyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)-6-methylpyridin-2-yl)-2-(isopropylamino)pyrimidin-4(3H)-one,(R)-2-((1-methoxypropan-2-yl)amino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, (R)-2-(2-(2-(methoxymethyl)pyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, (S)-2-(3-(dimethylamino)pyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(ethylamino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-(ethylamino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 2-((2-methoxyethyl)amino)-3-methyl-5-(6-methyl-5-((2 -(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-((tetrahydro-2H-pyran-4-yl)amino)pyrimidin-4(3H)-one, 2-(tert-butylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4 -yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one, 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(neopentylamino)pyrimidin-4(3H)-one, and 2-(3,3-difluoropyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one. Working Example

[0182] The present disclosure is further illustrated by the following examples, which should not be construed as limiting the scope or spirit of the present disclosure to the specific procedures described herein. It should be understood that the examples are provided to illustrate certain embodiments, and that no limitations to the scope of the present disclosure are intended thereby. It should be further understood that various other embodiments, modifications, and equivalents thereof that may suggest themselves to those skilled in the art can be resorted to without departing from the spirit of the present disclosure and / or the scope of the appended claims. [ka] Example A1. A solution of 3-hydroxy-2-methylpyridine (20.0 g, 183 mmol) and Na2CO3 (38.8 g, 367 mmol) in H2O (320 mL) and MeOH (200 mL) was treated with I2 (46.5 g, 183 mmol) and stirred at room temperature for 1 h. The mixture was acidified with HCl (2M), extracted with EtOAc (2 times), and the combined organics were washed with brine, dried over Na2SO4, and concentrated to dryness. The material was suspended in 1:1 EtOAc / Hex, sonicated, and the solid was collected by filtration and dried. The filtrate was concentrated to dryness, treated with DCM, and the solid was collected by filtration and combined with the first solid to give 6-iodo-2-methylpyridin-3-ol (20.5 g, 48%). MS (ESI) m / z: 236.0 (M+H + ).

[0183] A mixture of 6-iodo-2-methylpyridin-3-ol (6.8 g, 28.9 mmol), 2,4-dichloropyridine (8.56 g, 57.9 mmol), and K2CO3 (4.00 g, 28.9 mmol) in DMA (50 mL) was heated at 110 °C under argon for 16 h. The mixture was cooled to room temperature, treated with HO, extracted with EtOAc (2x), and the combined organics were washed with HO, then brine, dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (EtOAc / Hex) to give 3-((2-chloropyridin-4-yl)oxy)-6-iodo-2-methylpyridine (7.35 g, 73%) as a white solid. MS (ESI) m / z: 346.9 (M+H + ). [ka] Example A2. A solution of H2SO4 (12 mL) at 0°C was treated with H2O2 (9.72 mL, 95 mmol), stirred for 10 min, treated with a solution of 2-amino-5-fluoro-4-methylpyridine (2 g, 15.86 mmol) in H2SO4 (8 mL), stirred for 15 min, then warmed to room temperature and stirred for 3 h. The mixture was recooled to 0°C and gradually neutralized with solid NaHCO3, and the resulting solid was collected by filtration and dried to give 5-fluoro-4-methyl-2-nitropyridine (2 g, 81%). 1 H NMR(400MHz,DMSO-d6):δ 8.57(s,1H),8.42(d,J=5.3Hz,1H),2.42(d,J=1.9Hz,3H);MS(ESI)m / z:157.1(M+H + ).

[0184] A mixture of 5-fluoro-4-methyl-2-nitropyridine (2 g, 12.81 mmol) and 2-chloro-4-hydroxypyridine (1.66 g, 12.81 mmol) in DMF (26 mL) was sparged with Ar, treated with K2CO3 (2.66 g, 19.22 mmol), and heated at 88° C. for 24 h and then at 50° C. for 2 days. The mixture was treated with water and the resulting solid was collected by filtration and dried to give 5-((2-chloropyridin-4-yl)oxy)-4-methyl-2-nitropyridine (2.72 g, 80%). 1 H NMR(400MHz,DMSO-d6):δ 8.49(s,1H),8.47(s,1H),8.35(d,J=5.7Hz,1H),7.24(d,J=2.3Hz,1H),7.12(dd,J=5.7,2.3Hz,1H),2.32(s,3H);MS(ESI)m / z:266.0(M+H + ).

[0185] A solution of 5-((2-chloropyridin-4-yl)oxy)-4-methyl-2-nitropyridine (1.5 g, 5.65 mmol) and 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-piazole (1.527 g, 7.34 mmol) in dioxane (20 mL) was sparged with Ar, treated with a solution of K2CO3 (1.171 g, 8.47 mmol) in water (5 mL) and Pd(PPh3)4 (0.326 g, 0.282 mmol) and heated at 80 °C overnight. The mixture was cooled to room temperature, treated with water, extracted with DCM (4 times) and the combined organics were dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give 4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)-2-nitropyridine (2.3 g, 75%). 1 H NMR(400MHz,DMSO-d6):δ 8.48(s,1H),8.43-8.42(m,2H),8.27(s,1H),7.98(s,1H),7.30(d,J=2.4Hz,1H) ,6.83(dd,J=5.7,2.4Hz,1H),3.84(s,3H),2.34(s,3H);MS(ESI)m / z:312.1(M+H+ ).

[0186] A solution of 4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)-2-nitropyridine (2.3 g, 7.39 mmol) in MeOH (37 mL) and THF (37 mL) was treated with NHCl (11.86 g, 222 mmol) followed by dropwise addition of zinc dust (4.83 g, 73.9 mmol) and the mixture was stirred at room temperature overnight. The mixture was diluted with EtOAc, the solids were removed by filtration through diatomaceous earth, and the filtrate was concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give 4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-amine (1.4 g, 67%). MS (ESI) m / z: 282.1 (M+H) + ). [ka] Example A3. A solution of Example A2 (0.2 g, 0.71 mmol) in dibromomethane (5 mL) was treated with tetrabutylammonium bromide (0.92 g, 2.84 mmol) and t-butyl nitrite (0.7 g, 7.11 mmol) and stirred at room temperature for 4 h. The mixture was diluted with EtOAc, washed successively with saturated NaHCO3, water, and brine, dried over Na2SO4, and concentrated to dryness to give 2-bromo-4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridine as an off-white solid (0.21 g, 86%). MS (ESI) m / z: 345.1 (M+H) + ). [ka] Example A4. A suspension of Example A2 (0.62 g, 2.2 mmol), KI (3.7 g, 22.3 mmol), and diiodomethane (5 mL, 62 mmol) was treated with t-butyl nitrite (1.4 mL, 11.7 mmol) and stirred at room temperature for 12 h. The mixture was treated with EtOAc and washed successively with saturated NaHCO3 (3 times), 10% Na2S2O3 (2 times), and brine (2 times), and the combined aqueous washes were back-extracted with EtOAc (2 times). The combined organics were dried over MgSO4, concentrated to dryness, and purified by silica gel chromatography (EtOAc / DCM) to give 2-iodo-4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridine (0.51 g, 59%). MS (ESI) m / z: 393.0 (M+H + ). [ka] Example A5. A solution of 5-bromo-2-nitropyridine (15 g, 73.9 mmol) in DMF (300 mL) was sparged with Ar, treated with Cs2CO3 (48.2 g, 148 mmol) and 2-chloro-4-hydroxypyridine (10.53 g, 81 mmol), sparged with Ar again and heated at 85 °C overnight. The mixture was cooled to room temperature, filtered through a bed of silica gel, washed thoroughly with EtOAc, and the filtrate was treated with 5% LiCl and stirred overnight. The layers were separated, the aqueous layer was extracted with additional EtOAc (4 times), and the combined organics were dried over Na2SO4 and concentrated to dryness. The residue was dissolved in EtOAc, treated with 5% LiCl, stirred for 1 h, the layers were separated, and the aqueous layer was extracted with EtOAc (3 times). The combined organics were dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (EtOAc / Hex). The material was suspended in MTBE, sonicated, and the resulting solid was collected by filtration to give 2-chloro-4-((6-nitropyridin-3-yl)oxy)pyridine (6.06 g, 33%). 1H NMR(400MHz,DMSO-d6):δ 8.62(d,J=2.4,1H),8.43-8.39(m,2H),8.06(dd,J=8.8,2.8Hz,1H),7.36(d,J=2.0 Hz,1H),7.23(dd,J=5.6,2.0Hz,1H);MS(ESI)m / z:252.0(M+H+).

[0187] A solution of 2-chloro-4-((6-nitropyridin-3-yl)oxy)pyridine (20.0 g, 79 mmol) in MeOH (40 mL) was hydrogenated in the presence of Raney Nickel (2.00 g, 34.1 mmol) at 40 psi for 3 h. The catalyst was removed by filtration, rinsed with MeOH, and the filtrate was concentrated to dryness to give 5-((2-chloropyridin-4-yl)oxy)pyridin-2-amine (18.52 g, 105%) as a brown solid. MS (ESI) m / z: 222.0 (M+H) + ).

[0188] A mixture of 5-((2-chloropyridin-4-yl)oxy)pyridin-2-amine (1.00 g, 4.51 mmol) and potassium iodide (3.74 g, 22.5 mmol) in DCM (15 mL) was treated dropwise with t-butyl nitrite (4.65 g, 45.1 mmol) and the mixture was stirred overnight at room temperature. The mixture was diluted with EtOAc (75 mL), washed with 10% Na2CO3 (50 mL), then with water (50 mL) and finally with brine (50 mL) and dried over sodium sulfate. The solvent was evaporated under reduced pressure to give a viscous oily solution. EtOAc (100 mL) was added and the solution was washed with 0.1 M sodium thiosulfate (75 mL), brine (50 mL) and dried over sodium sulfate. The solvent was evaporated under reduced pressure and the residual oil was purified by silica gel chromatography to provide 2-chloro-4-((6-iodopyridin-3-yl)oxy)pyridine (695 mg, 46%). 1H NMR(400MHz,DMSO-d6):δ 8.80(d,J=3.0Hz,1H),8.73(d,J=5.8Hz,1H),8.35(d,J=8.6Hz,1H),7.91(dd,J=8.6,3.1Hz,1H),7.61(d,J=2.3Hz,1H),7.48(dd,J=5.8,2.3Hz,1H). [ka] Example A6. DMF (25 mL) was slowly treated with SOCl2 (125 mL) while maintaining the temperature at 40-50 °C. The mixture was then treated dropwise with pyridine-2-carboxylic acid (25 g, 0.2 mol) over 0.5 h, then heated at reflux for 16 h, cooled to room temperature, diluted with toluene (80 mL) and concentrated to dryness (this process was repeated three times). The resulting residue was washed with toluene and dried under reduced pressure to give 4-chloro-pyridine-2-carbonyl chloride (27.6 g, 79% yield), which was used in the next step without purification.

[0189] A 0° C. solution of 4-chloro-pyridine-2-carbonyl chloride (27.6 g, 0.16 mol) in THF (100 mL) was treated dropwise with a solution of MeNH2 in EtOH and stirred at 3° C. for 4 h before being concentrated to dryness. The material was suspended in EtOAc, the solids removed by filtration, and the filtrate was washed with brine (2×) and concentrated to give 4-chloro-N-methylpicolinamide (16.4 g, 60%) as a yellow solid. 1 H NMR(400MHz,DMSO-d6)δ 8.78(br s,1H),8.55(d,J=5.2Hz,1H),7.97(d,J=2.0Hz,1H),7.66(m,1H),2.82(d,J=4.8Hz,3H);MS(ESI)m / z:171.0(M+H + ).

[0190] A solution of 2-amino-5-hydroxypyridine (0.968 g, 8.79 mmol) in DMA (15 mL) was treated with tert-butoxide (0.987 g, 8.79 mmol), stirred at room temperature for 3 h, and then treated with 4-chloro-N-methylpicolinamide (1.5 g, 8.79 mmol) and stirred at room temperature for 2 days. The mixture was concentrated to dryness, treated with water, extracted with EtOAc (3 times), and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (EtOAc, MeOH / DCM) to give 4-((6-aminopyridin-3-yl)oxy)-N-methylpicolinamide (1.3 g, 61%). 1 H NMR(400MHz,DMSO-d6):δ 8.75(m,1H),8.46(d,J=5.6Hz,1H),7.82(d,J=2.9Hz,1H),7.34(d,J=2.6Hz,1H),7.30(dd,J=8.9,3.0Hz,1H), 7.10(dd,J=5.6,2.7Hz,1H),6.53(d,J=8.9Hz,1H),6.07(s,2H),2.77(d,J=4.8Hz,3H);MS(ESI)m / z:245.1(M+H + ).

[0191] A mixture of 4-((6-aminopyridin-3-yl)oxy)-N-methylpicolinamide (0.4 g, 1.64 mmol) and potassium iodide (1.36 g, 8.19 mmol) in methylene iodide (5.46 mL) was treated dropwise with t-butyl nitrite (1.95 mL, 16.4 mmol). The mixture was stirred at room temperature overnight, diluted with EtOAc (75 mL), washed with 10% sodium carbonate (50 mL), 10% thiosulfate (50 mL), and brine (50 mL). The organics were dried over sodium sulfate, evaporated to dryness, and purified by silica gel chromatography ((EtOAc / Hex) to give 4-((6-iodopyridin-3-yl)oxy)-N-methylpicolinamide (0.214 g, 36.8%). 1H NMR(400MHz,DMSO-d6):δ 8.78(br d,J=5.6Hz,1H),8.54(d,J=5.6Hz,1H),8.40(d,J=3.0Hz,1H),7.95(d,J=8.6Hz,1H),7.50(dd,J=8.6,3.1 Hz,1H),7.45(d,J=2.6Hz,1H),7.23(dd,J=5.6,2.7Hz,1H),2.78(d,J=4.9Hz,3H);MS(ESI)m / z:356.0(M+H + ). [ka] Example B1. A solution of 5-bromo-2-chloro-4-methoxypyrimidine (0.6 g, 2.69 mmol) in THF (10 mL) was treated with ethylamine (2.0 M in THF, 6.71 mL, 13.43 mmol), heated at 60° C. for 1 h, cooled to room temperature and diluted with water. The mixture was extracted with EtOAc (2×) and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness and purified by column chromatography (EtOAc / Hex) to give 5-bromo-N-ethyl-4-methoxypyrimidin-2-amine as a white solid (0.54 g, 87%). MS (ESI) m / z: 232.03 (M+H + ).

[0192] A solution of 5-bromo-N-ethyl-4-methoxypyrimidin-2-amine (0.73 g, 3.15 mmol) in dioxane (25 mL) was sparged with Ar, treated with bis(pinacolato)diborane (1.04 g, 4.1 mmol), KOAc (0.62 g, 6.29 mmol), PdCl2(dppf) (0.23 g, 0.315 mmol), and heated at 100 °C for 20 h. The mixture was cooled to room temperature to give crude (2-(ethylamino)-4-methoxypyrimidin-5-yl)boronic acid (estimated 50% yield), which was used without further purification. MS (ESI) m / z: 198.1 (M+H) + ). [ka] Example B2. A solution of 5-bromo-4-methoxy-2-(methylthio)pyrimidine (1.0 g, 4.25 mmol), bis(pinacolato)diborane (1.30 g, 5.10 mmol), and KOAc (1.25 g, 12.76 mmol) in dioxane (10 mL) was sparged with Ar, treated with PdCl2(dppf)-DCM adduct (0.17 g, 0.21 mmol), sparged with Ar again, and heated at 85° C. overnight. The mixture was cooled to room temperature, treated with saturated NaHCO3, extracted with EtOAc (3 times), and the combined organics were dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (MeOH / EtOAc) to give (4-methoxy-2-(methylthio)pyrimidin-5-yl)boronic acid pinacol ester (estimated yield 100%), which was used without further purification. MS(ESI)m / z:201.1(M+H + ). [ka] Example B3. A solution of 5-bromo-2-chloro-4-methoxypyrimidine (1.2 g, 5.37 mmol) in THF (20 mL) was treated with TEA (1.57 mL, 10.74 mmol) and isopropylamine (0.7 mL, 8.1 mmol) and heated at 60° C. for 5 h. The mixture was cooled to room temperature, treated with water, extracted with EtOAc (2×), and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (EtOAc / Hex) to give 5-bromo-N-isopropyl-4-methoxypyrimidin-2-amine (0.84 g, 634%). 1 H NMR(400MHz,DMSO-d6):δ 8.10(s,1H),7.17(br s,1H),3.96-3.94(m,1H),3.87(s,3H),1.12(d,J=6.5Hz,6H);MS(ESI)m / z:246.0(M+H + ).

[0193] A solution of 5-bromo-N-isopropyl-4-methoxypyrimidin-2-amine (0.84 g, 3.41 mmol) in dioxane (20 mL) was sparged with Ar, treated with bis(pinacolato)diborane (1.127 g, 4.44 mmol), KOAc (0.502 g, 5.12 mmol), and PdCl2(dppf)-DCM adduct (0.279 g, 0.341 mmol), and heated at 95 °C for 16 h. The mixture was cooled to room temperature and concentrated to dryness to give crude N-isopropyl-4-methoxy-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyrimidin-2-amine (estimated 60% yield), which was used without further purification. MS (ESI) m / z: 212.1 (M+H) + ) [the corresponding boronic acid ion]. [ka] Example B4. A suspension of 2-(methylthio)pyrimidin-4(3H)-one (2.0 g, 14.1 mmol) in DMA (40 mL) at 0° C. was treated with solid LiHMDS (3.06 g, 18.3 mmol) followed by methyl iodide (1.14 mL, 18.3 mmol), warmed to room temperature and stirred overnight. The mixture was quenched with water, extracted with EtOAc (3×), and the combined organics were dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (EtOAc / Hex) to give 3-methyl-2-(methylthio)pyrimidin-4(3H)-one (1.37 g, 62%). 1 H NMR(400MHz,DMSO-d6):δ 7.83(d,J=6.5Hz,1H),6.17(d,J=6.5Hz,1H),3.39(s,3H),2.54(s,3H);MS(ESI)m / z:157.1(M+H + ).

[0194] A solution of 3-methyl-2-(methylthio)pyrimidin-4(3H)-one (1.37 g, 8.77 mmol) in CHCl3 (15 mL) at 0 °C was treated with bromine (0.54 mL, 10.5 mmol), stirred at 0 °C for 1 h, quenched with saturated NaHCO3 (15 mL), gradually warmed to room temperature and stirred overnight. The mixture was extracted with DCM (3 times) and the combined organics were dried over Na2SO4 and concentrated to dryness to give 5-bromo-3-methyl-2-(methylthio)pyrimidin-4(3H)-one (2.0 g, 97% yield). 1 H NMR(400MHz,DMSO-d6):δ 8.24(s,1H),3.45(s,3H),2.55(s,3H);MS(ESI)m / z:235.0(M+H + ).

[0195] A mixture of 5-bromo-3-methyl-2-(methylthio)pyrimidin-4(3H)-one (1.0 g, 4.25 mmol), bis(pinacolato)diborane (1.30 g, 5.10 mmol), and KOAc (1.25 g, 12.7 mmol) in dioxane (10 mL) was sparged with Ar, treated with PdCl(dppf)-DCM adduct (0.17 g, 0.21 mmol), sparged again with Ar and heated at 85 °C overnight. The mixture was cooled to room temperature, quenched with saturated NaHCO3, extracted with EtOAc (3x), and the combined organics were dried over Na2SO4 and concentrated to dryness to give 3-methyl-2-(methylthio)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyrimidin-4(3H)-one (assumed 100% yield). MS (ESI) m / z: 202.1 (boronic acid + H + mass). [ka] Example C1. A solution of crude Example B1 (0.310 g, 1.574 mmol) in dioxane (25 mL) was treated with a degassed solution of K2CO3 (0.65 g, 4.72 mmol), Example A1 (0.55 g, 1.58 mmol), and Pd(PPh3)4 (0.18 g, 0.16 mmol) in water (4 mL) and heated at 90° C. for 16 h. The mixture was cooled to room temperature, diluted with water, extracted with EtOAc (3 times), and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness, and purified by column chromatography (EtOAc / DCM) to give 5-(5-((2-chloropyridin-4-yl)oxy)-6-methylpyridin-2-yl)-N-ethyl-4-methoxypyrimidin-2-amine (350 mg, 60%). 1 H NMR(400MHz,DMSO-d6):δ 8.73(s,1H),8.29(d,J=5.8Hz,1H),7.77(d,J=8.9Hz,1H),7.61(d,J=8.6Hz,1H),7.38(br s,1H),7.06(d,J=2.3Hz,1H),6.94(dd,J=5.8,2.3Hz,1H),4.01-3.91(m,3H), 3.36-3.34(m,2H),2.33(s,3H),1.15(t,J=7.4Hz,3H);MS(ESI)m / z:372.1(M+H + ). [ka] Example C2. A mixture of B2 (0.63 g, 2.25 mmol), Example A1 (0.65 g, 1.88 mmol), and K2CO3 (0.78 g, 5.63 mmol) in 5:1 dioxane / HO (6 mL) was sparged with Ar, treated with Pd(PPh3)4 (0.22 g, 0.19 mmol), sparged again with Ar, and heated at 90 °C overnight. The mixture was cooled to room temperature, treated with saturated NaHCO3, extracted with EtOAc (3x), and the combined organics were dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (EtOAc / Hex) to give 5-(5-((2-chloropyridin-4-yl)oxy)-6-methylpyridin-2-yl)-4-methoxy-2-(methylthio)pyrimidine (0.49 g, 70%). 1H NMR(400MHz,DMSO-d6):δ 8.91(s,1H),8.31(d,J=5.8Hz,1H),7.89(d,J=8.5Hz,1H),7.72(d,J=8.5Hz,1H),7.11(d,J=2.3Hz ,1H),6.99(dd,J=5.8,2.3Hz,1H),4.06(s,3H),2.58(s,3H),2.39(s,3H);MS(ESI)m / z:375.1(M+H + ).

[0196] A mixture of 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole (0.30 g, 1.44 mmol), 5-(5-((2-chloropyridin-4-yl)oxy)-6-methylpyridin-2-yl)-4-methoxy-2-(methylthio)pyrimidine (0.49 g, 1.31 mmol), and K2CO3 (0.54 g, 3.92 mmol) in 5:1 dioxane / water (18 mL) was sparged with Ar, treated with Pd(PPh3)4 (0.15 g, 0.13 mmol), sparged again with Ar, and heated at 90 °C for 4 h. The mixture was cooled to room temperature, treated with saturated NaHCO3, extracted with EtOAc (3x) and the combined organics were dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give 4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(methylthio)pyrimidine (0.54 g, 98%). 1 H NMR(400MHz,DMSO-d6):δ 8.93(s,1H),8.39(d,J=5.7Hz,1H),8.28(s,1H),7.99(d,J=0.7Hz,1H),7.88(d,J=8.5Hz,1H),7.65(d,J=8.6Hz,1H),7.30 (d,J=2.4Hz,1H),6.66(dd,J=5.7,2.4Hz,1H),4.06(s,3H),3.85(s,3H),2.58(s,3H),2.42(s,3H);MS(ESI)m / z:421.1(M+H + ). [ka] Example C3. A mixture of Example B4 (0.35 g, 1.73 mmol), Example A1 (0.50 g, 1.44 mmol), and K2CO3 (0.60 g, 4.33 mmol) in 5:1 dioxane / water (12 mL) was sparged with Ar, treated with Pd(PPh3)4 (0.17 g, 0.14 mmol), sparged again with Ar, and heated at 90 °C overnight. The mixture was quenched with saturated NaHCO3, extracted with EtOAc (3x), and the combined organics were dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (EtOAc / Hex) to give 5-(5-((2-chloropyridin-4-yl)oxy)-6-methylpyridin-2-yl)-3-methyl-2-(methylthio)pyrimidin-4(3H)-one (0.52 g, 67%). MS(ESI)m / z:375.1(M+H + ).

[0197] A mixture of 5-(5-((2-chloropyridin-4-yl)oxy)-6-methylpyridin-2-yl)-3-methyl-2-(methylthio)pyrimidin-4(3H)-one (0.52 g, 0.97 mmol), 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole (0.22 g, 1.07 mmol), and KCO (0.40 g, 2.9 mmol) in 5:1 dioxane / water (6 mL) was sparged with Ar, treated with Pd(PPh) (0.12 g, 0.10 mmol), sparged again with Ar, and heated at 90 °C overnight. The solids were removed by filtration, the filtrate was treated with saturated NaHCO3, extracted with EtOAc (x3), and the combined organics were dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (MeOH / DCM) to give 3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(methylthio)pyrimidin-4(3H)-one (140 mg, 34%). MS (ESI) m / z: 421.1 (M+H) + ). [ka] Example C4. A solution of Example B3 (0.698 g, 2.381 mmol) in dioxane (20 mL) was treated with Example A1 (0.7 g, 2.4 mmol), a solution of K2CO3 (0.22 g, 1.6 mmol) in water (6 mL), and Pd(PPh3)4 (0.18 g, 0.16 mmol), sparged with Ar, and heated at 90° C. overnight. The mixture was cooled to room temperature, treated with water, extracted with EtOAc (×2), and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (EtOAc / Hex) to give 5-(5-((2-chloropyridin-4-yl)oxy)-6-methylpyridin-2-yl)-N-isopropyl-4-methoxypyrimidin-2-amine (0.48 g, 55%). MS(ESI)m / z:386.2(M+H + ). [ka] Example 1. A solution of Example C1 (0.12 g, 0.26 mmol) and 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole (0.070 g, 0.35 mmol) in dioxane (4 mL) was sparged with Ar and treated with a solution of K2CO3 (0.07 g, 0.51 mmol), Pd(PPh3)4 (0.03 g, 0.026 mmol) in water (1 mL) and heated at 90 °C for 2 h. The mixture was cooled to room temperature, diluted with water, extracted with EtOAc (2x) and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness and purified by column chromatography (MeOH / DCM) to give N-ethyl-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine as a white solid (77 mg, 71%). 1H NMR(400MHz,DMSO-d6):δ 8.74(s,1H),8.36(d,J=5.7Hz,1H),8.26(s,1H),7.97(d,J=0.7Hz,1H),7.76(d,J=8.5Hz,1H),7.55(d,J=8.6Hz,1H),7.36(br s,1H),7.25(d,J=2.4Hz,1H),6.61(dd,J=5.7,2.4Hz,1H),3.96(s,3H),3.84(s,3H),3.40-3.30(m,2H),2.37(s,3H),1.14(br m,3H);MS(ESI)m / z:418.2(M+H + ).

[0198] A mixture of N-ethyl-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.29 g, 0.7 mmol) and 48% aqueous HBr (0.32 mL, 2.78 mmol) in acetic acid (5 mL) was heated at 90° C. for 6 h. The mixture was cooled to room temperature, diluted with water (60 mL), basified with solid NaHCO3, extracted with 1:1 EtOAc / THF (3 times), and the combined organics were washed with brine, dried over Na2SO4, and concentrated to dryness. The residue was stirred with MeCN for 1 h and the resulting solid was collected by filtration to give 2-(ethylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one as a white solid (210 mg, 75%). 1 H NMR(400MHz,DMSO-d6):δ 11.20(br s,1H),8.67(s,1H),8.35(d,J=5.7Hz,1H),8.24-8.26(m,2H),7.96(s,1H),7.50(d,J=8.6Hz,1H),7.21(d,J=2.4Hz,1H),6.88(br s,1H),6.60(dd,J=5.7,2.4Hz,1H),3.84(s,3H),3.34-3.32(m,2H),2.34(s,3H),1.12(t,J=7.2Hz,3H);MS(ESI)m / z:404.2(M+H + ). [ka] Example 2. A solution of Example C2 (0.13 g, 0.309 mmol) in DCM (5 mL) was treated dropwise with mCPBA (0.09 g, 0.37 mmol), stirred at room temperature overnight, treated with TEA (0.5 mL) and N,N-dimethylamine HCl salt (500 mg) and stirred at room temperature for 2 h. The mixture was treated with saturated NaHCO3, extracted with DCM (2 times), and the combined organics were dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (MeOH / DCM) to give 4-methoxy-N,N-dimethyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (60 mg, 47%). MS (ESI) m / z: 418.2 (M+H + ).

[0199] A solution of 4-methoxy-N,N-dimethyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.060 g, 0.144 mmol) in acetic acid (5 mL) was treated with HBr (0.065 mL, 0.575 mmol), heated at 90 °C for 6 h, cooled to rt and quenched with ice-water. The solution was treated with NaHCO3 and NaCl, extracted with 1:1 THF / EtOAc (3x) and the combined organics were dried over Na2SO4 and concentrated to dryness. The material was treated with MeCN (1 mL), allowed to stand at room temperature, and the resulting solid was collected by filtration to give 2-(dimethylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (43 mg, 71%). 1H NMR(400MHz,DMSO-d6):δ 11.23(s,1H),8.73(s,1H),8.36(d,J=5.7Hz,1H),8.30(m,1H),8.26(s,1H),7.97(s,1H),7.51(m,1H),7.23(d,J=2.4Hz,1H),6.62(br s,1H),3.85(s,3H),3.12(s,6H),2.35(s,3H);MS(ESI)m / z:404.2(M+H + ). [ka] Example 3. A solution of Example C2 (0.13 g, 0.309 mmol) in DCM (5 mL) was treated dropwise with mCPBA (0.09 g, 0.37 mmol), stirred at room temperature overnight, and treated with isopropylamine (0.5 mL) and stirred at room temperature overnight. The mixture was treated with saturated NaHCO3, extracted with DCM (2 times), and the combined organics were dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (MeOH / DCM) to give N-isopropyl-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (63 mg, 47%). MS (ESI) m / z: 432.2 (M+H + ).

[0200] A solution of N-isopropyl-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.063 g, 0.14 mmol) in acetic acid (5 mL) was treated with HBr (0.066 mL, 0.58 mmol), heated at 90 °C for 4 h, cooled to rt and quenched with ice-water. The solution was treated with NaHCO3 and NaCl, extracted with 1:1 THF / EtOAc (3x) and the combined organics were dried over Na2SO4 and concentrated to dryness. The material was treated with MeCN (1 mL) and allowed to stand at room temperature and the resulting solid was collected by filtration to give 2-(isopropylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (25 mg, 38%). 1 H NMR(400MHz,DMSO-d6):δ 10.8(br s,1H),8.68(s,1H),8.36(d,J=5.7Hz,1H),8.27(s,1H),8.26(s,1H),7.96(s,1H),7.51(d,J=8.6Hz,1H),7.22(d,J=2.4Hz,1H),6.67(br s,1H),6.61(d,J=5.6Hz,1H),4.07(m,1H),3.85(s,3H),2.34(s,3H),1.17(d,J=6.5Hz,6H);MS(ESI)m / z:418.2(M+H) + ). [ka] Example 4. A solution of Example C1 (0.15 g, 0.4 mmol) and 2-methyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridine (0.13 g, 0.57 mmol) in dioxane (4 mL) was sparged with Ar, treated with a solution of K2CO3 (0.11 g, 0.8 mmol) in water (1 mL) and heated at 90 °C for 2 h. The mixture was cooled to room temperature, diluted with water, extracted with EtOAc (3x) and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give N-ethyl-4-methoxy-5-(6-methyl-5-((6′-methyl-[2,3′-bipyridine]-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine as an orange foam (0.106 g, 61%). 1 H NMR (400MHz, DMSO-d6): δ 9.11(d,J=2.4Hz,1H),8.74(s,1H),8.54(d,J=5.7Hz,1H),8.29(dd,J=8.1,2.4Hz ,1H),7.77(d,J=8.6Hz,1H),7.65(d,J=2.4Hz,1H),7.59(d,J=8.6Hz,1H),7.34(d ,J=8.2Hz,1H),7.02(d,J=1.9Hz,1H),6.79(dd,J=5.7,2.4Hz,1H),3.96(s,3H),3 .36-3.34(m,2H),2.51(s,3H),2.38(s,3H),1.15(s,3H);MS(ESI)m / z:429.2(M+H + ).

[0201] Using the procedure of Example 1, N-ethyl-4-methoxy-5-(6-methyl-5-((6′-methyl-[2,3′-bipyridine]-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.13 g, 0.3 mmol) and 48% HBr (0.66 mL, 12 mmol) were combined to give 2-(ethylamino)-5-(6-methyl-5-((6′-methyl-[2,3′-bipyridine]-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one as a white solid (0.09 g, 73%). 1H NMR(400MHz,DMSO-d6):δ 11.08(s,1H),9.10(s,1H),8.68(s,1H),8.53(d,J=5.7Hz,1H),8.28(d,J=8. 3Hz,2H),7.62(s,1H),7.54(d,J=8.7Hz,1H),7.34(d,J=8.2Hz,1H),6.82(br s,1H),6.78(d,J=5.7Hz,1H),3.35-3.32(m,2H),2.50(s,3H),2.35(s,3H),1.12(t,J=7.2Hz,3H);MS(ESI)m / z:415.2(M+H + ). [ka] Example 5. A mixture of Me4tBuXPhos (0.018 g, 0.043 mmol) and Pd2(dba)3 (0.020 g, 0.022 mmol) in dioxane (1 mL) was sparged with Ar, heated at 100 °C for several minutes, treated with Example C1 (0.16 g, 0.43 mmol), 4-methyl-1H-imidazole (0.1 g, 1.3 mmol), and K3PO4 (0.18 g, 0.86 mmol), and heated at 100 °C for 20 h. The mixture was cooled at room temperature, diluted with EtOAc, and the solids were removed by filtration through diatomaceous earth and washed with EtOAc. The filtrate was washed with water, then brine, dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give N-ethyl-4-methoxy-5-(6-methyl-5-((2-(4-methyl-1H-imidazol-1-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine as a white solid (0.12 g, 67%). 1H NMR(400MHz,DMSO-d6):δ 8.75(s,1H),8.40(d,J=1.4Hz,1H),8.32(d,J=5.8Hz,1H),7.77(d,J=8.7Hz,1H),7.65(s,1H),7.59(d,J=8.6Hz,1H),7.49(br s,1H),7.42(d,J=2.2Hz,1H),6.74(dd,J=5.8,2.2Hz,1H),3.96(s,3H),3.36 -3.34(m,2H),2.37(s,3H),2.13(s,3H),1.14(s,3H);MS(ESI)m / z:418.2(M+H + ).

[0202] Using the procedure of Example 1, N-ethyl-4-methoxy-5-(6-methyl-5-((2-(4-methyl-1H-imidazol-1-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.12 g, 0.29 mmol) and 48% HBr (0.63 mL, 11.5 mmol) were combined to give 2-(ethylamino)-5-(6-methyl-5-((2-(4-methyl-1H-imidazol-1-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one as a white solid (0.06 g, 51%). 1 H NMR(400MHz,DMSO-d6):δ 10.60(br s,1H),8.68(s,1H),8.40(d,J=1.3Hz,1H),8.31-8.30(m,2H),7.64(s,1H),7.54(d,J=8.6Hz,1H),7.39(d,J=2.2Hz,1H),6.93(br s,1H),6.73(dd,J=5.8,2.2Hz,1H),3.35-3.33(m,2H),2.35(s,3H),2.13(s,3H),1.12(t,J=7.1Hz,3H);MS(ESI)m / z:404.2(M+H + ). [ka] Example 6. A solution of Example C2 (0.15 g, 0.36 mmol) in DCM (5 mL) was treated dropwise with mCPBA (0.11 g, 0.43 mmol), stirred at room temperature overnight, treated with 2-methoxyethanamine (0.5 mL) and stirred at room temperature for 4 h. The mixture was treated with saturated NaHCO3, extracted with DCM (3 times), and the combined organics were dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give 4-methoxy-N-(2-methoxyethyl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (100 mg, 63%). MS (ESI) m / z: 448.2 (M+H + ).

[0203] A solution of 4-methoxy-N-(2-methoxyethyl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.10 g, 0.22 mmol) in acetic acid (5 mL) was treated with HBr (0.10 mL, 0.90 mmol), heated at 90 °C for 4 h, cooled to room temperature and quenched with ice-water. The solution was treated with NaHCO3 and NaCl, extracted with EtOAc (3x) and the combined organics were dried over Na2SO4 and concentrated to dryness. The material was treated with MeCN (3 mL) and the resulting solid was collected by filtration to give 2-((2-methoxyethyl)amino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (65 mg, 61%). 1H NMR(400MHz,DMSO-d6):δ 11.02(br s,1H),8.67(s,1H),8.35(d,J=5.7Hz,1H),8.29-8.23(m,2H),7.96(d,J=0.7Hz,1H),7.50(d,J=8.6Hz,1H),7.22(d,J=2.4Hz,1H),6.85(br s,1H),6.60(dd,J=5.7,2.4Hz,1H),3.84(s,3H),3.53-3.44(m,4H),3.28(s,3H),2.34(s,3H);MS(ESI)m / z:434.2(M+H + ). [ka] Example 7. A solution of Example C2 (0.15 g, 0.357 mmol) in DCM (5 mL) was treated dropwise with mCPBA (0.11 g, 0.43 mmol), stirred at room temperature overnight, treated with methylamine (2.0 M in THF, 3.6 mL, 7.2 mmol) and stirred at room temperature for 4 h. The mixture was treated with saturated NaHCO3, extracted with DCM (3 times), and the combined organics were dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give 4-methoxy-N-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (100 mg, 70%). MS (ESI) m / z: 404.2 (M+H + ).

[0204] A solution of 4-methoxy-N-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.10 g, 0.25 mmol) in acetic acid (5 mL) was treated with HBr (0.12 mL, 1.0 mmol) and heated at 90° C. for 4 h. The mixture was cooled to room temperature, quenched with ice water, treated with NaHCO3 and NaCl, and extracted with EtOAc (3 times). The combined organics were dried over Na2SO4 and concentrated to dryness, and the resulting material was treated with MeCN (3 mL) and the solid was collected by filtration to give 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(methylamino)pyrimidin-4(3H)-one (52 mg, 53%). 1 H NMR(400MHz,DMSO-d6):δ 11.26(s,1H),8.70(s,1H),8.36(d,J=5.7Hz,1H),8.28(m,1H),8.26(s,1H),7.97(s,1H),7.51(d,J=8.6Hz,1H),7.22(d,J=2.4Hz,1H),6.70(br s,1H),6.61(dd,J=5.7,2.4Hz,1H),3.85(s,3H),2.85(d,J=4.7Hz,3H),2.35(s,3H);MS(ESI)m / z:390.2(M+H + ). [ka] Example 8. A mixture of Example B1 (0.20 g, 0.51 mmol), Example A5 (0.17 g, 0.51 mmol), and K2CO3 (0.21 g, 1.52 mmol) in dioxane (6 mL) and water (1.5 mL) was sparged with Ar, treated with Pd(PPh3)4 (0.06 g, 0.051 mmol), sparged with Ar again, and heated at 90 °C for 7 h. The mixture was cooled to room temperature, treated with saturated NaHCO3, extracted with EtOAc (3 times), and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (EtOAc / Hex) to give 5-(5-((2-chloropyridin-4-yl)oxy)pyridin-2-yl)-N-ethyl-4-methoxypyrimidin-2-amine (90 mg, 50%). MS(ESI)m / z:358.1(M+H + ).

[0205] A mixture of 5-(5-((2-chloropyridin-4-yl)oxy)pyridin-2-yl)-N-ethyl-4-methoxypyrimidin-2-amine (0.090 g, 0.25 mmol), 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole (0.060 g, 0.28 mmol), and KCO (0.10 g, 0.76 mmol) in 5:1 dioxane / water (6 mL) was sparged with Ar, treated with Pd(PPh) (0.03 g, 0.025 mmol), sparged again with Ar, and heated at 90 °C for 7 h. The mixture was cooled to room temperature, quenched with saturated NaHCO3, extracted with EtOAc (3x) and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give N-ethyl-4-methoxy-5-(5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (80 mg, 79%). MS (ESI) m / z: 404.2 (M+H). + ).

[0206] Using the procedure of Example 7, N-ethyl-4-methoxy-5-(5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.080 g, 0.20 mmol) was combined with acetic acid (3 mL) and HBr (0.090 mL, 0.79 mmol) to give 2-(ethylamino)-5-(5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (40 mg, 51%). 1 H NMR(400MHz,DMSO-d6):δ 11.12(s,1H),8.66(s,1H),8.43(m,2H),8.38(d,J=5.7Hz,1H),8.26(s,1H),7.9 7(d,J=0.7Hz,1H),7.62(dd,J=8.8,2.9Hz,1H),7.28(d,J=2.4Hz,1H),63.80(br s,1H),6.72(dd,J=5.7,2.4Hz,1H),3.85(s,3H),3.35(m,2H),1.12(t,J=7.2Hz,3H);MS(ESI)m / z:390.2(M+H + ). [ka] Example 9. A solution of Example C2 (0.15 g, 0.36 mmol) in DCM (5 mL) was treated with mCPBA (0.11 g, 0.43 mmol), stirred at room temperature for 2 h, treated with pyrrolidine (0.5 mL) and stirred at room temperature overnight. The mixture was treated with saturated NaHCO3, extracted with DCM (3 times), and the combined organics were dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give 4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidine (100 mg, 63%). MS (ESI) m / z: 444.2 (M+H + ).

[0207] A solution of 4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidine (0.1 g, 0.22 mmol) in acetic acid (3 mL) was treated with HBr (0.1 mL, 0.90 mmol) and heated at 90° C. for 6.5 h. The mixture was cooled to room temperature, quenched with ice water, neutralized to pH 8 with NaHCO3, and extracted with EtOAc (3×). The combined organics were dried over Na2SO4 and concentrated to dryness, and the resulting material was treated with MeCN and the solid collected by filtration to give 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidin-4(3H)-one (89 mg, 91%). 1 H NMR(400MHz,DMSO-d6):δ 11.21(s,1H),8.73(s,1H),8.36(d,J=5.7Hz,1H),8.31(m,1H),8.26(s,1H),7.97(d,J=0.7Hz,1H),7.52(m,1H),7.22 (d,J=2.4Hz,1H),6.61(d,J=5.6Hz,1H),3.85(s,3H),3.50(m,4H),2.35(s,3H),1.91(m,4H);MS(ESI)m / z:430.2(M+H + ). [ka] Example 10. A mixture of Example C3 (0.14 g, 0.33 mmol) and isopropylamine (3 mL, 35.0 mmol) was heated in a sealed tube at 100° C. for 2 days. The mixture was cooled to room temperature, the solids were removed by filtration, and the filtrate was concentrated to dryness and purified by silica gel chromatography to give 2-(isopropylamino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (88 mg, 59%). 1H NMR(400MHz,DMSO-d6):δ 8.68(s,1H),8.36(d,J=5.7Hz,1H),8.28(d,J=8.7Hz,1H),8.25(s,1H), 7.96(d,J=0.7Hz,1H),7.52(d,J=8.6Hz,1H),7.23(d,J=2.4Hz,1H),7.05 (d,J=7.6Hz,1H),6.60(dd,J=5.7,2.4Hz,1H),4.33(m,1H),3.85(s,3H),3.37(s,3H),2.35(s,3H),1.23(d,J=6.6Hz,6H);MS(ESI)m / z:432.2(M+H) + ). [ka] Example 11. A suspension of PdCl2(dppf)·CH2Cl2 (0.048 g, 0.059 mmol), KOAc (0.086 g, 0.879 mmol), bis(pinacolato)diboron (0.186 g, 0.732 mmol), and 5-bromo-N-isopropyl-4-methoxypyrimidin-2-amine (0.144 g, 0.586 mmol) in dioxane (6 mL) was sparged with Ar and heated at 90 °C for 20 h. The mixture was cooled to room temperature, treated with additional PdCl2(dppf)·CH2Cl2 (0.048 g, 0.059 mmol), KOAc (0.086 g, 0.879 mmol), and bis(pinacolato)diboron (0.186 g, 0.732 mmol), sparged with Ar and heated at 100 °C for 20 h. The mixture was cooled to room temperature, treated with Pd(PPh3)4 (0.034 g, 0.029 mmol), K2CO3 (0.121 g, 0.879 mmol), Example A6 (0.104 g, 0.293 mmol), and water (1.5 mL), sparged with Ar, heated at 85 °C for 18 h, and then cooled to room temperature. The mixture was treated with saturated NaHCO3, extracted with EtOAc (4 times) and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (EtOAc / DCM) to give 4-((6-(2-(isopropylamino)-4-methoxypyrimidin-5-yl)pyridin-3-yl)oxy)-N-methylpicolinamide (72 mg, 62%). MS (ESI) m / z: 395.2 (M+H)+ ).

[0208] A solution of 4-((6-(2-(isopropylamino)-4-methoxypyrimidin-5-yl)pyridin-3-yl)oxy)-N-methylpicolinamide (0.072 g, 0.183 mmol) in DCE (10 mL) was treated with iodotrimethylsilane (0.497 mL, 3.65 mmol) and heated at 50° C. for 20 h, treated with additional iodotrimethylsilane (0.25 mL, 1.84 mmol) and heated at 60° C. for 20 h. The mixture was cooled at rt, treated with DCM / THF (5:1), washed with saturated NaHCO3, 10% sodium bisulfite, then brine. The combined aqueous washes were back-extracted with DCM / THF (5:1) (1x) and the combined organics were dried over Na2SO4, concentrated to dryness and purified by preparative TLC (EtOAc) to give 4-((6-(2-(isopropylamino)-6-oxo-1,6-dihydropyrimidin-5-yl)pyridin-3-yl)oxy)-N-methylpicolinamide (16 mg, 23%). 1 H NMR(400MHz,DMSO-d6):δ 10.89(br s,1H),8.79(d,J=5.2Hz,1H),8.66(s,1H),8.52(d,J=5.6Hz,1H),8.47(d,J=2.9Hz,1H),8.44(d,J= 8.8Hz,1H),7.67(dd,J=8.8,2.9Hz,1H),7.42(d,J=2.6Hz,1H),7.21(dd,J=5.6,2.6Hz,1H),6.74(br s,1H),4.08(m,1H),2.77(d,J=4.8Hz,3H),1.16(d,J=6.5Hz,6H);MS(ESI)m / z:381.2(M+H + ). [ka] Example 12. Using the procedure of Example 6, Example C2 (0.15 g, 0.357 mmol), mCPBA (0.106 g, 0.428 mmol), and morpholine (0.5 mL, 5.78 mmol) were combined to give 4-(4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-yl)morpholine (127 mg, 77%). MS (ESI) m / z: 460.2 (M+H + ).

[0209] Using the procedure of Example 6, 4-(4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-2-yl)pyrimidin-2-yl)morpholine (0.127 g, 0.276 mmol) and HBr (0.126 mL, 1.106 mmol) were combined in acetic acid (3 mL) to give 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-morpholinopyrimidin-4(3H)-one (75 mg, 59%). 1 H NMR(400MHz,DMSO-d6):δ 11.57(br s,1H),8.77(s,1H),8.36(d,J=5.7Hz,1H),8.27-8.22(m,2H),7.97(s,1H),7.59(m,1H),7.24(d,J=2.4H) z,1H),6.63(dd,J=5.7,2.4Hz,1H),3.84(s,3H),3.68-3.63(m,8H),2.36(s,3H);MS(ESI)m / z:446.2(M+H + ). [ka] Example 13. Using the procedure of Example 6, Example C2 (0.15 g, 0.357 mmol), mCPBA (0.106 g, 0.428 mmol), and piperidine (0.6 mL, 6.07 mmol) were combined to give 4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(piperidin-1-yl)pyrimidine (134 mg, 82%). MS (ESI) m / z: 458.2 (M+H + ).

[0210] Using the procedure of Example 6, 4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(piperidin-1-yl)pyrimidine (0.134 g, 0.293 mmol) and HBr (0.133 mL, 1.172 mmol) were combined in acetic acid (3 mL) to give 4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(piperidin-1-yl)pyrimidine (103 mg, 79%). 1 H NMR(400MHz,DMSO-d6):δ 11.33(br s,1H),8.71(br s,1H),8.35(d,J=5.7Hz,1H),8.31-8.18(m,2H),7.96(s,1H),7.53(br s,1H),7.23(d,J=2.4Hz,1H),6.61(m,1H),3.84(s,3H),3.68(m,4H),2.35(s,3H),1.61(m,2H),1.53(m,4H);MS(ESI)m / z:444.2(M+H) + ). [ka] Example 14. Using the procedure of Example 6, Example C2 (0.100 g, 0.238 mmol), mCPBA (0.070 g, 0.285 mmol), and cyclopropylamine (0.300 mL, 4.33 mmol) were combined to give N-cyclopropyl-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (82 mg, 80%). MS (ESI) m / z: 430.2 (M+H + ).

[0211] A solution of N-cyclopropyl-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.082 g, 0.191 mmol) in acetic acid (2 mL) was treated with HBr (0.087 mL, 0.764 mmol) and heated at 90° C. for 4 h. The mixture was cooled to room temperature, treated with ice, neutralized with saturated NaHCO3, and extracted with EtOAc (3 times). The combined organics were washed with brine, dried over MgSO4, concentrated to dryness, treated with MeCN, sonicated, heated to near boiling, and allowed to stand at room temperature overnight. The resulting solid was collected by filtration. The combined aqueous washings were filtered and the solid was washed with water, dried and combined with the solid isolated above to give 2-(cyclopropylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (35 mg, 44%). 1 H NMR(400MHz,DMSO-d6):δ 11.09(br s,1H),8.68(s,1H),8.35(d,J=5.7Hz,1H),8.28(m,1H),8.25(s,1H),7.96(d,J=0.8Hz,1H),7.66(br s,1H),7.52(d,J=8.6Hz,1H),7.22(d,J=2.4Hz,1H),6.60(dd,J=5.7,2.4Hz,1H),3.84(s,3 H),2.73-2.68(m,1H),2.34(s,3H),0.75(m,2H),0.55-0.53(m,2H);MS(ESI)m / z:416.2(M+H+ ). [ka] Example 15. Using the procedure of Example 6, Example C2 (0.100 g, 0.238 mmol), mCPBA (0.070 g, 0.285 mmol), and cyclopentylamine (0.400 mL, 4.04 mmol) were combined to give N-cyclopentyl-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (85 mg, 78%). MS (ESI) m / z: 458.2 (M+H + ).

[0212] Using the procedure of Example 6, N-cyclopentyl-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyrimidin-2-amine (0.085 g, 0.186 mmol) and HBr (0.085 mL, 0.743 mmol) were combined in acetic acid (2 mL) to give 2-(cyclopentylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (58 mg, 70%). 1 H NMR(400MHz,DMSO-d6):δ 10.78(br s,1H),8.67(s,1H),8.35(d,J=5.7Hz,1H),8.29-8.23(m,2H),7.96(s,1H),7.50(d,J=8.6Hz,1H),7.22(d,J=2.4Hz,1H),6.94(br s,1H),6.60(dd,J=5.7,2.4Hz,1H),4.21-4.18(m,1H),3.84(s,3H),2.34(s,3H),1.92-1.8 9(m,2H),1.68-1.64(m,2H),1.58-1.54(m,2H),1.49-1.40(m,2H);MS(ESI)m / z:444.2(M+H + ). [ka] Example 16. A solution of Example C3 (0.087 g, 0.207 mmol) in pyrrolidine (1.75 mL, 21.31 mmol) was heated at 100° C. in a sealed vessel overnight, cooled to room temperature, concentrated to dryness, and purified by silica gel chromatography (EtOAc, MeOH / DCM). The material was treated with MeCN, sonicated, and the resulting solid was collected by filtration and dried to obtain 3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidin-4(3H)-one (62 mg, 67%). 1 H NMR(400MHz,DMSO-d6):δ 8.65(s,1H),8.35(d,J=5.7Hz,1H),8.30(d,J=8.6Hz,1H),8.25(s,1H),7.96(s,1H),7.54(d,J=8.6Hz,1H),7.24(d,J=2.4Hz ,1H),6.60(dd,J=5.7,2.4Hz,1H),3.84(s,3H),3.58(m,4H),3.45(s,3H),2.35(s,3H),1.87(m,4H);MS(ESI)m / z:444.2(M+H + ). [ka] Example 17. Using the procedure of Example 16, Example C3 (0.087 g, 0.207 mmol) and cyclopropylamine (1.5 mL, 21.65 mmol) were combined to give 2-(cyclopropylamino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (49 mg, 55%). 1H NMR(400MHz,DMSO-d6):δ 8.70(s,1H),8.35(d,J=5.7Hz,1H),8.29-8.25(m,2H),7.96(s,1H),7.52(d,J=8.6Hz,1H),7.48(d,J=3.1Hz,1H),7.23(d,J=2.4Hz,1H),6.60( dd,J=5.7,2.4Hz,1H),3.84(s,3H),3.33(s,3H),2.88-2.87(m,1H),2.3 5(s,3H),0.77-0.71(m,2H),0.65-0.61(m,2H);MS(ESI)m / z:430.2(M+H + ). [ka] Example 18. A solution of Example B3 (0.13 g, 0.44 mmol) in dioxane (4 mL) was treated with a solution of Example A3 (0.09 g, 0.26 mmol), Pd(PPh3)4 (0.03 g, 0.026 mmol), K2CO3 (0.036 g, 0.26 mmol) in water (1 mL) and heated at 90° C. overnight. The mixture was cooled to room temperature, treated with water, extracted with EtOAc (2×), and the combined organics were washed with brine, dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (MeOH / DCM) to give N-isopropyl-4-methoxy-5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (55 mg, 49%). 1 H NMR(400MHz,DMSO-d6):δ 8.69(s,1H),8.35(d,J=5.7Hz,2H),8.26(s,1H),7.97(s,1H),7.84(s,1H),7.25(d,J=2.4Hz,1H),6.59(dd,J=5.7Hz, 2.5Hz,1H),4.15-4.12(m,1H),3.97(s,3H),3.84(s,3H),2.17(s,3H),1.175(d,J=6.4Hz,6H);MS(ESI)m / z:432.2(M+H) + ).

[0213] Using the procedure of Example 3, N-isopropyl-4-methoxy-5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.053 g, 0.12 mmol) and HBr (0.2 mL) were combined in acetic acid (3 mL) to give 2-(isopropylamino)-5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (0.03 g, 59%). 1 H NMR(400MHz,DMSO-d6):δ 10.86(s,1H),8.65(s,1H),8.34(m,2H),8.30(s,1H),8.25(s,1H),7.96(s,1H),7.21(d,J=2.4Hz,1H),6.63(br s,1H),6.58(dd,J=5.7,2.4Hz,1H),4.09-4.07(m,1H),3.84(s,3H),2.14(s,3H),1.16(d,J=6.5Hz,6H);MS(ESI)m / z:418.2(M+H + ). [ka] Example 19. A solution of Example C4 in dioxane (3 mL) was treated with acetamide (0.06 g, 1.0 mmol), Cs2CO3 (0.11 g, 0.34 mmol), X-Phos (0.03 g, 0.077 mmol), and Pd2(dba)3 (0.03 g, 0.034 mmol) and heated at 80° C. overnight. The mixture was diluted with EtOAc, the solids were removed by filtration through diatomaceous earth, washed with EtOAc, the filtrate was washed with water, then with brine, dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (MeOH / DCM) to give N-(4-((6-(2-(isopropylamino)-4-methoxypyrimidin-5-yl)-2-methylpyridin-3-yl)oxy)pyridin-2-yl)acetamide (60 mg, 36%). 1H NMR(400MHz,DMSO-d6):δ 10.56(s,1H),8.73(s,1H),8.18(d,J=5.7Hz,1H),7.76(d,J=8.4Hz,1H),7.62(s,1H),7.55(d,J=8.6Hz,1H),7.44-7.18(br m,1H),6.63(dd,J=5.7,2.4Hz,1H),4.10(m,1H),3.95(s,3H),2.33(s,3H),2.02(s,3H),1.17(d,J=6.4Hz,6H);MS(ESI)m / z:409.2(M+H + ).

[0214] A solution of N-(4-((6-(2-(isopropylamino)-4-methoxypyrimidin-5-yl)-2-methylpyridin-3-yl)oxy)pyridin-2-yl)acetamide (0.05 g, 0.12 mmol) in DCE (3 mL) was treated with TMS-I (0.5 mL, 3.67 mmol) and heated at 50 °C for 4 h. The mixture was diluted with 10% NaSO and 1:1 THF / EtOAc, stirred for several minutes, the layers were separated, and the aqueous layer was extracted with EtOAc (1x). The combined organics were washed with brine, dried over NaSO, concentrated to dryness, and purified by silica gel chromatography (MeOH / EtOAc) to give N-(4-((6-(2-(isopropylamino)-4-methoxypyrimidin-5-yl)-2-methylpyridin-3-yl)oxy)pyridin-2-yl)acetamide (0.018 g, 37%). 1 H NMR(400MHz,DMSO-d6):δ 10.79(s,1H),10.51(s,1H),8.61(s,1H),8.20(d,J=8.6Hz,1H),8.12(d,J=5.7Hz,1H),7.54(s,1H),7.47-7.46(m 1H),6.64(br s,1H),6.58(dd,J=5.8,2.4Hz,1H),4.03-3.99(m,1H),2.26(s,3H),1.97(s,3H),1.11(d,J=6.5Hz,6H);MS(ESI)m / z:395.2(M+H + ). [ka] Example 20. A suspension of Example A4 (0.25 g, 0.64 mmol), Example B2 (0.22 g, 0.78 mmol), and K2CO3 (0.27 g, 2.0 mmol) in dioxane (5 mL) and water (1 mL) was sparged with Ar, treated with Pd(PPh3)4 (0.070 g, 0.061 mmol), and heated at 90 °C for 16 h. The mixture was cooled to room temperature, treated with saturated NaHCO3, extracted with EtOAc (4 times), and the combined organics were dried over MgSO4, concentrated to dryness, and purified by silica gel chromatography (MeOH / DCM) to give 4-methoxy-5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(methylthio)pyrimidine (0.28 g, 99%). 1 H NMR(400MHz,DMSO-d6):δ 8.89(s,1H),8.47(s,1H),8.37(d,J=5.7Hz,1H),8.27(s,1H),7.98(d,J=5.7Hz,2H),7.28(d,J=2.4Hz,1H) ,6.63(dd,J=5.7,2.5Hz,1H),4.07(s,3H),3.85(s,3H),2.58(s,3H),2.22(s,3H);MS(ESI)m / z:421.1(M+H + ).

[0215] A solution of 4-methoxy-5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(methylthio)pyrimidine (0.28 g, 0.67 mmol) in DCM (10 mL) was treated with mCPBA (0.20 g, 0.80 mmol), stirred at room temperature for 3 h, treated with pyrrolidine (0.50 mL, 6.1 mmol) and stirred at room temperature overnight. The mixture was treated with saturated NaHCO3, extracted with DCM (3x) and the combined organics were dried over MgSO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give 4-methoxy-5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidine (0.19 g, 64%).1 H NMR(400MHz,DMSO-d6):δ 8.76(s,1H),8.37(s,1H),8.35(d,J=5.7Hz,1H),8.27(s,1H),7.97(s,1H),7.85(s,1H),7.25(d,J=2.4Hz,1H),6.60( dd,J=5.7,2.5Hz,1H),4.00(s,3H),3.84(s,3H),3.55(s,5H),2.18(s,3H),1.93-1.92(m,4H);MS(ESI)m / z:444.2(M+H + ).

[0216] A solution of 4-methoxy-5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidine (0.19 g, 0.43 mmol) in acetic acid (2.5 mL) was treated with HBr (48%, 0.10 mL, 1.8 mmol) and heated at 90° C. for 16 h. The mixture was poured onto ice (10 g), neutralized with saturated NaHCO3, and the resulting solid was collected by filtration, washed with water and MeCN, and dried to give 5-(4-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(pyrrolidin-1-yl)pyrimidin-4(3H)-one (0.11 g, 58%). 1 H NMR(400MHz,DMSO-d6):δ 11.22(bs,1H),8.68(s,1H),8.38(s,1H),8.34(d,J=5.7Hz,1H),8.30(d,J=1.3Hz,1H),8.26(s,1H),7.96(s,1H),7.2 2(d,J=2.4Hz,1H),6.60-6.58(m,1H),3.84(s,3H),3.50(bs,4H),2.15(s,3H),1.91(bs,4H);MS(ESI)m / z:430.2(M+H + ). [ka] Example 21. Example C4 (0.30 g, 0.778 mmol), 1-ethyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole (0.19 g, 0.85 mmol), and potassium carbonate (0.32 g, 2.33 mmol) were combined in a mixture of dioxane:H2 (4:1, 10 mL). The mixture was sparged with Ar, then tetrakis(triphenylphosphine)palladium(0) (0.090 g, 0.078 mmol) was added. The mixture was sparged again with Ar and heated at 90° C. overnight. The mixture was quenched with NaHCO3 and extracted with EtOAc (3×). The organics were dried over Na2SO4, filtered, and concentrated to give the crude product. The crude product was purified by silica gel chromatography (EtOAc / hexanes) to give 5-(5-((2-(1-ethyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)-6-methylpyridin-2-yl)-N-isopropyl-4-methoxypyrimidin-2-amine (0.25 g, 72.2% yield). 1H NMR(400MHz,DMSO-d6):δ 8.75(s,1H),8.37(d,J=5.7Hz,1H),8.32(s,1H),7.99(s,1H),7.77(m,1H),7.56(d,J=8.6Hz,1H),7.27(d,J=2.4Hz,1H),6.61(dd,J=5.7,2.4H z,1H),4.14(q,J=7.3Hz,2H),4.01(s,1H),3.95(m,3H),2.37(s,3H),1.38(t,J=7.3Hz,3H),1.18(d,J=6.5Hz,6H);MS(ESI)m / z:446.3(M+H+).

[0217] 5-(5-((2-(1-ethyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)-6-methylpyridin-2-yl)-N-isopropyl-4-methoxypyrimidin-2-amine (0.25 g, 0.56 mmol) was dissolved in AcOH (5 mL) and 48% hydrobromic acid (0.25 mL, 2.24 mmol) was added. The mixture was heated at 90° C. for 5 h. The mixture was evaporated under high vacuum. The residue was treated with NaHCO3 solution and the solution was extracted with EtOAc (3 times). The organics were dried over Na2SO4, filtered and concentrated. The residue was treated with hot MeCN. The solid was filtered and dried under vacuum to give 5-(5-((2-(1-ethyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)-6-methylpyridin-2-yl)-2-(isopropylamino)pyrimidin-4(3H)-one (185 mg, 73.1% yield). 1 H NMR(400MHz,DMSO-d6):δ 10.81(s,1H),8.68(s,1H),8.36(d,J=5.7Hz,1H),8.31(s,1H),8.26(d,J =8.6Hz,1H),7.98(s,1H),7.51(d,J=8.6Hz,1H),7.24(d,J=2.4Hz,1H),6. 60(dd,J=5.7,2.4Hz,1H),4.14(q,J=7.3Hz,2H),4.07(m,1H),2.34(s,3H) ,1.37(t,J=7.3Hz,3H),1.17(d,J=6.5Hz,6H);MS(ESI)m / z:432.2(M+H+). [ka] Example 22. Example C2 (0.15 g, 0.36 mmol) was dissolved in DCM (5 mL), then mCPBA (0.11 g, 0.43 mmol) was added in portions. The mixture was stirred at room temperature for 3 h. (S)-(+)-1-Methoxy-2-propylamine (0.45 mL) was added, and the mixture was stirred at room temperature for 2 days. The mixture was quenched with NaHCO3 solution, and the solution was extracted with DCM (2 times). The organics were dried over Na2SO4, filtered, and concentrated to give the crude product. The material was purified by silica gel chromatography (MeOH / DCM) to give (R)-4-methoxy-N-(1-methoxypropan-2-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (98 mg, 59.5% yield). MS(ESI)m / z:462.2(M+H + ).

[0218] (R)-4-Methoxy-N-(1-methoxypropan-2-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (98 mg, 0.21 mmol) was dissolved in AcOH (3 mL) and then hydrobromic acid (0.1 mL, 0.85 mmol) was added. The mixture was heated at 90° C. for 3.5 h. The mixture was concentrated and the residue was treated with NaHCO3 solution. The solution was extracted with EtOAc (3 times). The organics were washed with NaHCO3, dried over Na2SO4, filtered and concentrated to give the crude. The crude was treated with hot MeCN and kept at room temperature. The solid was filtered, washed with MeCN and dried under vacuum to give (R)-2-((1-methoxypropan-2-yl)amino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (67 mg, 59.2% yield). 1H NMR(400MHz,DMSO-d6):δ 10.85(s,1H),8.67(s,1H),8.36(d,J=5.7Hz,1H),8.25(m,2H),7.97(s,1H),7.51(d,J=8.6Hz,1H),7.23(d,J=2.4Hz,1H),6.75(br s,1H),6.60(dd,J=5.7,2.4Hz,1H),4.19(m,1H),3.85(s,3H),3.38(m,2H) ,3.30(s,3H),2.34(s,3H),1.15(d,J=6.7Hz,3H);MS(ESI)m / z:448.2(M+H + ). [ka] Example 23. Example C2 (0.10 g, 0.24 mmol) was dissolved in DCM (5 mL), then mCPBA (60 mg, 0.24 mmol) was added in portions. The mixture was stirred at room temperature for 2 h. (R)-2-(methoxymethyl)pyrrolidine (0.28 g, 2.38 mmol) was added, then the mixture was stirred at room temperature overnight. The mixture was quenched with NaHCO3 solution, and the solution was extracted with DCM (2 times). The organics were dried over Na2SO4, filtered, and concentrated to give the crude product. The material was purified by silica gel chromatography (MeOH / DCM) to give (R)-4-methoxy-2-(2-(methoxymethyl)pyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidine (96 mg, 83% yield). MS(ESI)m / z:488.3(M+H + ).

[0219] (R)-4-Methoxy-2-(2-(methoxymethyl)pyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidine (96 mg, 0.20 mmol) was dissolved in AcOH (3 mL) and then 48% hydrobromic acid (0.1 mL) was added. The mixture was heated at 90° C. for 3.5 h. The mixture was concentrated and the residue was treated with NaHCO3 solution. The solution was extracted with EtOAc (3 times) and the organics were washed with NaHCO3, dried over Na2SO4, filtered and concentrated to give the crude. The material was treated with ether and sonicated. The solid was filtered, washed with ether and dried under vacuum to give (R)-2-(2-(methoxymethyl)pyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (58 mg, 60.1% yield). 1 H NMR(400MHz,DMSO-d6):δ 11.15(br s,1H),8.73(s,1H),8.36(d,J=5.7Hz,1H),8.28(br s,1H),8.26(s,1H),7.97(s,1H),7.53(m,1H),7.23(d,J=2.4Hz,1H),6.62(m,1H) ,4.34(m,1H),3.85(s,3H),3.52-3.40(m,4H),3.28(s,3H),2.35(s,3H),1.95(br d,J=27.5Hz,4H);MS(ESI)m / z:474.3(M+H + ). [ka] Example 24. Example C2 (0.10 g, 0.24 mmol) was dissolved in DCM (5 mL), then mCPBA (60 g, 0.24 mmol) was added. The mixture was stirred at room temperature for 2.5 h. (3S)-(-)-3-(dimethylamino)pyrrolidine (0.27 g, 2.37 mmol) was added, then the mixture was stirred at room temperature overnight. The mixture was quenched with NaHCO3 and extracted with DCM (2 times). The organics were dried over Na2SO4, filtered, and concentrated to give the crude product. The material was purified by silica gel chromatography (MeOH / DCM) to give (S)-1-(4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-yl)-N,N-dimethylpyrrolidin-3-amine (100 mg, 86% yield). MS (ESI) m / z: 487.3 (M+H) + ).

[0220] (S)-1-(4-Methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-yl)-N,N-dimethylpyrrolidin-3-amine (0.10 g, 0.21 mmol) was dissolved in AcOH (3 mL) and then 48% hydrobromic acid (0.1 mL) was added. The mixture was heated at 90° C. for 3.5 h. The mixture was concentrated and the residue was treated with NaHCO3 solution. The solution was extracted with EtOAc (3 times) and the organics were washed with NaHCO3, dried over Na2SO4, filtered and concentrated to give the crude. The crude was treated with hot MeCN and kept at room temperature. The solid was filtered, washed with MeCN, and dried under vacuum to give (S)-2-(3-(dimethylamino)pyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (62 mg, 62.8% yield). 1H NMR(400MHz,DMSO-d6):δ 8.73(s,1H),8.36(d,J=5.7Hz,1H),8.29(m,1H),8.26(s,1H),7.97(s,1H),7.53(d,J=8.6Hz,1H),7.23(d,J=2.4Hz,1H),6.62(dd,J=5.7,2.4 Hz,1H),3.85(s,3H),3.74(m,2H),3.44(m,1H),3.31(s,3H),3.22(m,1 H),2.75(m,1H),2.35(s,3H),2.18(s,6H),2.11(m,1H),1.77(m,1H)one proton is missing; MS(ESI) m / z: 473.3 (M+H + ). [ka] Example 25. To a microwave vessel was added Example C3 (0.11 g, 0.26 mmol) followed by 2.0 M ethylamine in THF (10 mL, 20.00 mmol). The mixture was heated at 100° C. for 2 days. The mixture was concentrated to give a crude product. The crude product was purified by silica gel chromatography (MeOH / DCM) to give 2-(ethylamino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (90 mg, 82% yield). 1 H NMR(400MHz,DMSO-d6):δ 8.69(s,1H),8.39(d,J=5.9Hz,1H),8.29(m,2H),8.01(s,1H),7.56(m,2H),7.30(br s,1H),6.68(br s,1H),3.86(s,3H),3.45(m,2H),3.36(s,4H),2.36(s,3H),1.18(t,J=7.1Hz,3H);MS(ESI)m / z:418.2(M+H + ). [ka] Example 26. To a microwave vessel was added Example C3 (0.11 g, 0.26 mmol) followed by 2-methoxyethanamine (3 mL, 34.5 mmol). The mixture was heated at 100° C. for 2 days. The mixture was concentrated to give a crude product. The crude product was purified by silica gel chromatography (MeOH / DCM) to give 2-((2-methoxyethyl)amino)-3-methyl-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (98 mg, 84% yield). 1 H NMR(400MHz,DMSO-d6):δ 8.68(s,1H),8.37(d,J=5.8Hz,1H),8.28(m,2H),7.99(s,1H),7.57(t,J=5.6Hz,1H),7.54(d,J=8.6Hz,1H),7.27(s ,1H),6.64(m,1H),3.85(s,3H),3.56-3.54(m,4H),3.37(s,3H),3.28(s,3H),2.35(s,3H);MS(ESI)m / z:448.2(M+H + ). [ka] Example 27. A solution of Example C2 (0.200 g, 0.476 mmol) in DCM (5 mL) was treated with mCPBA (0.141 g, 0.571 mmol), stirred at room temperature for 3 hours, treated with 4-aminotetrahydropyran hydrochloride (0.524 g, 3.81 mmol) and TEA (0.530 mL, 3.81 mmol) and stirred at room temperature overnight. Additional 4-aminotetrahydropyran hydrochloride (0.524 g, 3.81 mmol) and TEA (0.530 mL, 3.81 mmol) were added and the mixture was stirred at room temperature for an additional 24 hours. The mixture was concentrated to dryness, transferred to a sealed vessel containing DMF (10 mL), treated with additional 4-aminotetrahydropyran hydrochloride (0.524 g, 3.81 mmol) and TEA (0.530 mL, 3.81 mmol) and heated at 60° C. overnight. The mixture was cooled to room temperature and the solids were removed by filtration and washed with DCM. The filtrate was treated with saturated NaHCO3, extracted with DCM (3x) and the combined organics were washed with 5% LiCl (3x) then brine, dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give 4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-N-(tetrahydro-2H-pyran-4-yl)pyrimidin-2-amine (125 mg, 56%). MS (ESI) m / z: 474.2 (M+H) + ).

[0221] A solution of 4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-N-(tetrahydro-2H-pyran-4-yl)pyrimidin-2-amine (0.125 g, 0.264 mmol) in DCE (3 mL) was treated with TMS-I (1.078 mL, 7.92 mmol) and heated at 60° C. for 5 h, then cooled to room temperature and stirred overnight. The mixture was treated with 10% Na2S2O3 and 1:1 THF / EtOAc, stirred for 0.5 h, the layers separated, the aqueous layer extracted with EtOAc (1×), the combined organics washed with brine, dried over MgSO4 and concentrated to dryness. The material was treated with MeCN, heated to near reflux and left at room temperature over the weekend. The solids were removed by filtration, and the filtrate was concentrated to dryness and purified by silica gel chromatography (MeOH / EtOAc). The resulting material was treated with 10% MeOH / DCM, filtered to remove the solids, and concentrated to dryness to give 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(tetrahydro-2H-pyran-4-yl)amino)pyrimidin-4(3H)-one (7.7 mg, 6%). 1 H NMR(400MHz,DMSO-d6):δ10.96(br s,1H),8.67(s,1H),8.35(d,J=5.7Hz,1H),8.29-8.24(m,2H),7.96(s,1H),7.51(d,J=8.6Hz,1H),7.22(d,J=2.4Hz,1H),7.00(br s,1H),6.60(dd,J=5.7,2.4Hz,1H),3.98(m,1H),3.87-3.80(m,5H),3.39(m,2H),2.34(s,3H),1.85(m,2H),1.47(m,2H);MS(ESI)m / z:460.2(M+H) + ). [ka] Example 28. A solution of Example C2 (0.100 g, 0.238 mmol) in DCM (5 mL) was treated with mCPBA (0.059 g, 0.238 mmol), stirred at room temperature for 2 hours, treated with t-butylamine (0.4 mL, 3.81 mmol), and stirred at room temperature overnight. Additional t-butylamine (0.4 mL, 3.81 mmol) was added, and the mixture was stirred at room temperature for an additional 24 hours. The mixture was concentrated to dryness, transferred to a sealed vessel containing DMF (5 mL), treated with additional t-butylamine (0.4 mL, 3.81 mmol), and heated at 60° C. overnight. The mixture was cooled to room temperature, and the solid was removed by filtration and washed with DCM. The filtrate was treated with saturated NaHCO3 and extracted with DCM (3x) and the combined organics were washed with 5% LiCl (3x) then brine, dried over Na2SO4, concentrated to dryness and purified by silica gel chromatography (MeOH / DCM) to give N-(tert-butyl)-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (69 mg, 65%). MS (ESI) m / z: 446.3 (M+H) + ).

[0222] A solution of N-(tert-butyl)-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-2-amine (0.069 g, 0.155 mmol) in acetic acid (1.5 mL) was treated with HBr (48% aqueous, 0.071 mL, 0.623 mmol) and heated at 90° C. for 4 h. The mixture was removed from the heat, treated with ice and EtOAc, neutralized with saturated NaHCO3, extracted with 1:1 EtOAc / THF (3 times), the combined organics washed with brine, dried over MgSO4 and concentrated to dryness. The resulting material was treated with MeCN, heated to near reflux and left at room temperature overnight. The solids were removed by filtration and the filtrate was concentrated to dryness and purified by silica gel chromatography (MeOH / DCM then MeOH / EtOAc) to give 2-(tert-butylamino)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (11 mg, 15%). 1 H NMR(400MHz,DMSO-d6):δ 10.59(s,1H),8.67(s,1H),8.37(d,J=5.6Hz,1H),8.27-8.22(m,2H),7.97(s,1H),7.52(d,J=8.9Hz,1H),7.23(d,J= 2.6Hz,1H),6.60(dd,J=6.1,2.6Hz,1H),6.52(s,1H),3.84(s,3H),2.34(s,3H),1.41(s,9H);MS(ESI)m / z:432.2(M+H + ). [ka] Example 29. A solution of 2,2,2-trimethylacetamide (1.0 g, 9.89 mL) in THF (100 mL) was slowly treated with LiAIH4 (2.0 M in THF, 14.83 mL, 29.7 mmol) and stirred overnight at room temperature under Ar. The mixture was slowly quenched with water (1.125 mL), 20% KOH (1.125 mL), and water (2.25 mL), stirred vigorously for 10 min, treated with Na2SO4, and the solids were removed by filtration through Celite, and the filter pad was washed with THF. The filtrate was treated with 1M HCl in MeOH (15 mL) and concentrated to dryness to give 2,2-dimethylpropan-1-amine hydrochloride (413 mg, 34%). 1 H NMR (400MHz, DMSO-d6): δ 7.88 (br s, 2H), 2.57 (s, 2H), 0.93 (s, 9H).

[0223] A solution of Example C2 (0.100 g, 0.238 mmol) in DCM (5 mL) was treated with mCPBA (0.059 g, 0.238 mmol), stirred at room temperature for 2 h, treated with 2,2-dimethylpropan-1-amine hydrochloride (0.410 g, 3.32 mmol) and TEA (0.464 mL, 3.33 mmol) and stirred at room temperature for 5 days. The mixture was treated with saturated NaHCO3, extracted with DCM (3 times), and the combined organics were dried over Na2SO4, concentrated to dryness, and purified by silica gel chromatography (MeOH / DCM) to give 4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-N-neopentylpyrimidin-2-amine (81 mg, 74%). MS(ESI)m / z:460.3(M+H + ).

[0224] A solution of 4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-N-neopentylpyrimidin-2-amine (0.081 g, 0.176 mmol) in acetic acid (2 mL) was treated with HBr (48% aqueous, 0.080 mL, 0.705 mmol) and heated at 80° C. for 6 h, then cooled to room temperature overnight. The mixture was treated with ice and EtOAc, neutralized with saturated NaHCO3, extracted with EtOAc (3 times), and the combined organics were washed with brine, dried over MgSO4, and concentrated to dryness. The material was suspended in MeCN and heated to near reflux and left at room temperature for 3 h. The resulting solid was collected by filtration to give 5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)-2-(neopentylamino)pyrimidin-4(3H)-one (44 mg, 56%). 1 H NMR(400MHz,DMSO-d6):δ 10.84(s,1H),8.68(s,1H),8.35(d,J=6.6Hz,1H),8.28-8.23(m,2H),7.96(s,1H),7.52(d,J=8.6Hz,1H),7.22(d,J=3.0Hz,1H),6.73(br s,1H),6.60(dd,J=5.1,2.3Hz,1H),3.86(s,3H),3.22(d,J=6.2Hz,2H),2.34(s,3H),0.91(s,9H);MS(ESI)m / z:446.2(M+H + ). [ka] Example 30. Example C2 (0.10 g, 0.24 mmol) was dissolved in DCM (5 mL) and then mCPBA (60 mg, 0.24 mmol) was added in portions. The mixture was stirred at room temperature for 5 days (the reaction was very slow). The mixture was transferred to a microwave vessel and heated at 40° C. for 2 days. The mixture was quenched with NaHCO3 and the solution was extracted with DCM (2 times). The organics were dried over Na2SO4, filtered and concentrated to give the crude product. The material was purified by silica gel chromatography (MeOH / DCM) to give 2-(3,3-difluoropyrrolidin-1-yl)-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidine (80 mg, 70% yield). MS (ESI) m / z: 480.2 (M+H + ).

[0225] A solution of 2-(3,3-difluoropyrrolidin-1-yl)-4-methoxy-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidine (0.080 g, 0.167 mmol) in AcOH (3 mL) was treated with 48% hydrobromic acid (0.1 mL). The mixture was heated at 90° C. for 3 h. The mixture was concentrated and the residue was treated with NaHCO3 and EtOAc. The mixture was stirred at room temperature. The solid was filtered, washed with water, EtOAc and dried under vacuum to give 2-(3,3-difluoropyrrolidin-1-yl)-5-(6-methyl-5-((2-(1-methyl-1H-pyrazol-4-yl)pyridin-4-yl)oxy)pyridin-2-yl)pyrimidin-4(3H)-one (42 mg, 52.3% yield). 1H NMR (400 MHz, DMSO-d6): δ 8.76 (br s, 1H), 8.36 (d, J = 5.7 Hz, 1H), 8.28 (br s, 1H), 8.27 (s, 1H), 7.97 (s, 1H), 7.58 (br s, 1H), 7.24 (d, J = 2.4 Hz, 1H), 6.64 (s, 1H), 3.96 (m, 2H), 3.85 (s, 3H), 3.76 (m, 2H), 2.54 (m, 2H), 2.37 (s, 3H), one proton missing; MS (ESI) m / z: 466.2 (M+H + ).

[0226] The following assays demonstrate that certain compounds of formula I inhibit the kinase activity of c-FMS kinase, c-KIT kinase, or PDGFRβ kinase in enzyme assays, and also inhibit the activity of c-FMS kinase in M-NFS-60 and THP-1 cell lines. In vivo evaluation of certain compounds of formula I also demonstrates inhibition of c-FMS in pharmacodynamic models, or exhibits efficacy in anterior tibial implant models, U-251 or GL-261 glioblastoma models, or in MDA-MB-231 breast cancer xenograft models. uFMS Kinase (SEQ ID NO:1) Assay

[0227] The activity of unphosphorylated c-FMS kinase (uFMS, SEQ ID NO: 1) was determined following the production of ADP from the FMS kinase reaction with ATP and polyE4Y as substrates by coupling with the pyruvate kinase / lactate dehydrogenase system (e.g., Schindler et al. Science (2000) 289:1938-1942). In this assay, the oxidation of NADH (and therefore the decrease at A340 nm) was continuously monitored spectrophotometrically. The reaction mixture (100 μL) contained FMS (purchased from Millipore) (10 nM), polyE4Y (1 mg / mL), MgCl2 (10 mM), pyruvate kinase (4 units), lactate dehydrogenase (0.7 units), phosphoenolpyruvate (1 mM), NADH (0.28 mM), and ATP (500 μM) in 90 mM Tris buffer containing 0.2% octyl-glucoside and 1% DMSO, pH 7.5. The inhibition reaction was initiated by mixing serially diluted test compounds with the above reaction mixture. The absorbance at 340 nm was continuously monitored for 4 h at 30 °C on a Synergy2 plate reader. The reaction rate was calculated using a time window of 3 to 4 h. The inhibition percentage was obtained by comparing the reaction rate with that of the control (i.e., in the absence of test compound). IC 50 Values ​​were calculated from a series of percent inhibition values ​​determined over a range of inhibitor concentrations using a software routine as implemented in the GraphPad Prism software package. uFMS kinase sequence used for screening (Y538 to end) (SEQ ID NO: 1) [Table 1]

[0228] uKit Kinase (SEQ ID NO:2) Assay The activity of unphosphorylated c-KIT kinase (uKIT, SEQ ID NO:2) was determined following the production of ADP from the KIT kinase reaction with ATP and polyE4Y as substrates by coupling with the pyruvate kinase / lactate dehydrogenase system (e.g., Schindler et al. Science (2000) 289:1938-1942). In this assay, the oxidation of NADH (and thus the decrease at A340 nm) was continuously monitored spectrophotometrically. The reaction mixture (100 μL) contained nonphosphorylated KIT (12 nM), polyE4Y (1 mg / mL), MgCl2 (10 mM), pyruvate kinase (4 units), lactate dehydrogenase (0.7 units), phosphoenolpyruvate (1 mM), as well as NADH (0.28 mM) and ATP (2000 μM) in 90 mM Tris buffer containing 0.2% octyl-glucoside and 1% DMSO, pH 7.5. The inhibition reaction was initiated by mixing serially diluted test compounds with the above reaction mixture. The absorbance at 340 nm was continuously monitored for 4 h at 30 °C on a Synergy2 plate reader (BioTech). The reaction kinetics over a 3-4 h time frame was used to determine the IC 50 The percent inhibition values ​​generated were calculated. uKit with N-terminal GST fusion used for screening (SEQ ID NO:2) [Table 2]

[0229] Unphosphorylated PDGFRβ (uPDGFRβ) Kinase (SEQ ID NO:3) Assay The activity of unphosphorylated PDGFRβ kinase (uPDGFRβ, SEQ ID NO: 3) was determined following the production of ADP from a kinase reaction with ATP and polyE4Y as substrates by coupling with the pyruvate kinase / lactate dehydrogenase system (e.g., Schindler et al. Science (2000) 289:1938-1942). In this assay, the oxidation of NADH (and thus the decrease at A340 nm) was continuously monitored spectrophotometrically. The reaction mixture (100 μL) contained PDGFRβ (DeCode, 15.7 nM), polyE4Y (2.5 mg / mL), MgCl2 (10 mM), pyruvate kinase (4 units), lactate dehydrogenase (0.7 units), phosphoenolpyruvate (1 mM), as well as NADH (0.28 mM) and ATP (500 μM) in 90 mM Tris buffer containing 0.2% octyl-glucoside and 1% DMSO, pH 7.5. The inhibition reaction was initiated by mixing serially diluted test compounds with the above reaction mixture. The absorbance at 340 nm was continuously monitored for 4 h at 30 °C on a Polarstar Optima or Synergy2 plate reader. The reaction rate was calculated using a time window of 1.5 to 2.5 h. The inhibition percentage was obtained by comparing the reaction rate with that of the control (i.e., without test compound). IC 50 Values ​​were calculated from a series of percent inhibition values ​​determined over a range of inhibitor concentrations using a software routine as implemented in the GraphPad Prism software package. uPDGFRβ kinase sequence used for screening (residues 557-1106) (SEQ ID NO:3) [Table 3]

[0230] Using the enzymatic protocols described above, compounds of Formula I were shown to be inhibitors in assays measuring the kinase activity of uFMS kinase, uKIT kinase, or uPDGFRβ kinase, as shown below in Table 1. Table 1. Activity of compounds of formula Ia in the uFMS kinase, uKIT kinase, or uPDGFRβ kinase enzyme assays. [Table 4]

[0231] M-NFS-60 cell culture M-NFS-60 cells (catalog #CRL-1838) were obtained from the American Type Culture Collection (ATCC, Manassas, VA). Briefly, cells were grown in suspension in RPMI 1640 medium supplemented with 10% characterized fetal bovine serum (Invitrogen, Carlsbad, CA), 0.05 mM 2-mercaptoethanol, and 20 ng / mL mouse recombinant macrophage colony-stimulating factor (M-CSF) at 37°C, 5% CO2, and 95% humidity. Cells were grown until they reached saturation, at which point they were subcultured or harvested for assay use.

[0232] M-NFS-60 cell proliferation assay Serial dilutions of test compounds were dispensed into 384-well black clear bottom plates (Corning, Corning, NY). 2500 cells were added per well in 50 μL of complete growth medium. Plates were incubated for 67 hours at 37° C., 5% CO2, and 95% humidity. At the end of the incubation period, 10 μL of a 440 μM solution of resazurin (Sigma, St. Louis, MO) in PBS was added to each well and incubated for an additional 5 hours at 37° C., 5% CO2, and 95% humidity. Plates were read on a Synergy2 reader (Biotek, Winooski, VT) using an excitation of 540 nM and an emission of 600 nM. IC 50 Values ​​were calculated from a series of percent inhibition values ​​determined over a range of inhibitor concentrations using a software routine as implemented in the GraphPad Prism software package.

[0233] THP-1 cell culture THP-1 cells (catalog #TIB-202) were obtained from ATCC. Briefly, cells were grown at 37°C, 5% CO2, and 95% humidity in RPMI 1640 supplemented with 10% characterized fetal bovine serum, 1% sodium pyruvate, 1% penicillin-streptomycin-glutamine (PSG), and 55 μM 2-mercaptoethanol (Invitrogen, Carlsbad, CA). Cells were grown until they reached 70-95% confluency, at which point they were subcultured or harvested for assay use. Phospho-FMS ELISA assay

[0234] Serial dilutions of test compounds were diluted 1:100 in assay medium (RPMI 1640 supplemented with 10% characterized fetal bovine serum) in 96-well black clear bottom plates (Corning, Corning, NY). In a separate 96-well black clear bottom plate, 150,000 THP-1 cells were added per well in 100 μL of assay medium. 50 μL of diluted compounds were then added to the cells. Plates were incubated for 4 hours at 37° C., 5% CO2, 95% humidity. At the end of the incubation period, cells were stimulated with 50 μL of a 100 μM solution of recombinant human M-CSF (catalog #216-MC, R&D Systems, Minneapolis, MN) in assay medium and plates were incubated for 5 minutes at 37° C., 5% CO2, 95% humidity. Lysates were prepared and used to perform phospho-FMS ELISA as described by the manufacturer (catalog #DYC3268, R&D Systems, Minneapolis, MN). ICs obtained from data generated from the ELISA assay were calculated using GraphPad Prism. 50 values ​​were calculated.

[0235] Osteoclast tartrate-resistant acid phosphatase assay Serial dilutions of test compounds were dispensed into 384-well black clear bottom plates (Nalge Nunc International, Rochester, NY). Compounds were diluted by the addition of DMEM medium supplemented with 10% characterized fetal bovine serum (Invitrogen, Carlsbad, CA). Diluted compounds were transferred to the 384-well black clear bottom plates. 2500 osteoclast precursors (Lonza, Walkersville, MD) were added per well in growth medium containing receptor activator of nuclear factor kappa-beta ligand (RANKL) and M-CSF (R&D Systems, Minneapolis, MN). Plates were incubated for 7-14 days at 37°C, 5% CO2, and 95% humidity to allow differentiation of osteoclast precursors. At the end of the incubation period, 10 μL of supernatant from each well was transferred to a clear 384-well plate. Tartrate-resistant acid phosphatase activity in the supernatant samples was determined using an acid phosphatase assay kit (Sigma, St. Louis, MO). Absorbance was measured at 550 nm using a plate reader. Data was analyzed using Prism software (Graphpad, San Diego, CA) to obtain IC 50 values ​​were calculated.

[0236] Compounds of formula I were demonstrated to be functional inhibitors in one or more of the above cellular assays, as shown in Table 2. Table 2. Inhibitory effects of compounds of formula I versus M-NFS-60, THP-1, and osteoclasts [Table 5]

[0237] Analysis of cFOS mRNA production in the c-FMS mouse spleen pharmacodynamic model To investigate the in vivo modulation of FMS activity by the compound of formula I, spleen samples were collected from female DBA / 1 mice and analyzed for M-CSF-stimulated cFOS mRNA production. Briefly, 6-7 week old female Taconic DBA / 1BO J Bom Tac mice were treated with a single oral dose (by gavage) of either vehicle or compound. Plasma and spleen samples were collected from 4 mice at each time point: 2, 4, 6, 8, 12, 18, and 24 hours after dosing. All mice were IV-injected with 1 μg (100 μL fixed volume) of M-CSF 15 minutes prior to euthanasia. M-CSF, recombinant murine macrophage colony-stimulating factor (36.4 kDa homodimer, purity ≥ 98%), was obtained from Gibco. All procedures performed in this experiment were performed in compliance with all National Institutes of Health (NIH) laws, regulations, and guidelines. cFOS mRNA levels in spleen extracts were determined using a quantitative reverse transcriptase PCR kit from Life Technologies. Plasma levels of FMS inhibitors were determined by mass spectrometry analysis. The degree of FMS inhibition correlated with the observed reduction in cFOS mRNA levels in spleen samples of treated animals compared to vehicle.

[0238] In this model, Examples 3, 9, and 10 conferred ≧70% inhibition of cFOS mRNA levels by 8 hours after a 30 mg / kg dose.

[0239] PC-3 pretibial bone transplant model of cancer bone metastasis To evaluate the in vivo anticancer activity of the compounds of formula I, a bone invasive model, PC-3 M-luc pre-tibial injection model, was used. Briefly, PC-3 M-luc cells were obtained from Xenogen Corporation (Caliper Life Sciences) and grown at 37°C in a 5% CO2 atmosphere in MEM medium modified with L-glutamine (Cell Gro® #10-045-CV) supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin-glutamine, 1% non-essential amino acids, and 1% MEM vitamins. Male nude mice (Crl:NU-Foxn1nu), 6-7 weeks of age, were obtained from Charles River Laboratories. Test mice were inoculated with 1x10 per mouse on day 0 using an insulin syringe with a fixed 28-gauge needle. 6 A single cell (0.1 mL) was implanted into the anterior tibia. A needle was inserted into the ankle between the tibia and fibula until the bevel of the needle reached approximately midway between the knee and ankle. Treatment began on day 0. Animals were dosed by oral gavage twice daily for the duration of the study. All procedures performed in this experiment were performed in compliance with all National Institutes of Health (NIH) laws, regulations, and guidelines. When primary tumors reached a size of approximately 800 mg, ex vivo micro-CT was performed on tumor-bearing fixed hind limb specimens using a GE RS150 small animal micro-CT scanning instrument with the following settings: X-ray tube voltage = 70kVp X-ray tube current=25mA Exposure time = 20 ms Number of frames = 500 Angle increment between frames = 0.4° Average number per frame = 2 How to obtain=Parker

[0240] Images were then reconstructed at high resolution (100 microns, isotropic). Isosurface volume rendering was used to delineate the hindlimb lesions. A constant threshold was used to produce a consistent representation of the isosurface between different anatomical sites and specimens. Lesions in the right hindlimb were scored with a value of 0, 1, 2, 3, or 4 based on a qualitative assessment of lesion size as defined below. 0: Normal bone 1: Minimal lesion. Some roughness of the isosurface. Small areas of obvious bone resorption. 2: Mild. More lesions. Significant isosurface roughness. Full thickness lesions evident. 3: Moderate. Larger and more numerous full thickness lesions. 4: Marked. Many large full thickness lesions. Significant distortion of remaining structures. Significant bone loss.

[0241] Example 10 was evaluated in this model at an oral dose of 30 mg / kg given twice daily for 39 days and showed a clear benefit with a lesion score of 2 compared to 4 in vehicle treated animals.

[0242] Intracerebroventricular implantation of U251 in mice To evaluate the in vivo anticancer activity of compounds of formula I in conjunction with fractionated, localized head irradiation, an orthotopic U251-luc (Luc) human glioblastoma cancer model in female outbred nu / nu mice is used. Briefly, U251 cells are obtained from ATCC and modified to express luciferase. They are grown in RPMI 1640 medium supplemented with 10% FBS and 1% PSG. The growth environment is maintained at 37°C in an incubator with a 5% CO2 atmosphere. Female Harlan nude mice (Hsd:AthymicNude-Fox1nu) 8-9 weeks old are used in this study. Test animals are intracranially implanted with U251-luc (LucmCherry) cells. Briefly, animals are subcutaneously injected with 5mg / kg carprofen and anesthetized using 2% isoflurane in air. The animal is then secured in a stereotaxic frame (ASlinstruments, Inc.) and a hole is burred 2 mm right lateral and 1 mm anterior to the coronal suture. The cell suspension (kept on wet ice) is thoroughly mixed and drawn up into a 50 μL syringe. The syringe needle is placed in the center of the burr hole and pushed 3 mm down into the brain and pulled back 1 mm to form a "reservoir" of cell suspension sediment. 10 μL of cell suspension (1 × 10 per mouse) is then added. 6Cells) are gradually injected into the brain tissue. Tumor progression is followed by in vivo bioluminescence imaging performed using an IVIS 50 optical imaging system (Xenogen, Alameda, CA). Bioluminescence images are acquired at regular intervals for tumor burden estimation. All procedures performed in this experiment are performed in compliance with all National Institutes of Health (NIH) laws, regulations, and guidelines. Treatment is initiated when the mean brain bioluminescence signal of all groups in the experiment is approximately 1.3×109 photons / sec (usually 9 days after implantation). All mice are irradiated with 2 Gy from a RadSource RS-2000 irradiator every day for 5 consecutive days. Additionally, mice receive test compounds administered by oral gavage or are optionally co-administered with bevacizumab by tail vein injection. Bioluminescence images are typically acquired on days 8, 10, 14, 17, 21, 22, 24, 28, and 35 after implantation for tumor burden estimation. For each measurement, each mouse is subcutaneously injected with 150 mg / kg D-luciferin (Promega) and imaged 10 minutes after injection. Images are analyzed using Living Image (Xenogen, Alameda, CA) software. BLI signal in the brain is calculated using a fixed area ROI to estimate tumor burden. The mean BLI signal of each group is compared to vehicle control to determine treatment benefit. 28 days after the first radiation treatment, mice are euthanized by carbon dioxide overexposure for blood and brain collection. Whole blood is collected by terminal cardiac puncture and placed into EDTA Microtainer® tubes. Brains are excised and placed in 10% neutral buffered formalin.

[0243] GL261 intracranial implantation model To evaluate the in vivo anticancer activity of compounds of formula I, intracranial implants of GL261-luc2 mouse glioblastoma are used. Briefly, GL261-luc2 cells are obtained from Caliper Life Sciences, Inc. and grown in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% FBS and 1% PSG. The growth environment is maintained at 37°C in an incubator with a 5% CO2 atmosphere. Following growth, the cells are resuspended using serum-free medium and diluted to 1×108 Generate a concentration of 1000 cells / mL. Six to seven week old female C57BL / 6J-Tyrc-2J / J from Jackson Labs are intracranially implanted with GL261-luc2 cells on day 0. For sterile surgical implantation, animals are subcutaneously injected with 5 mg / kg carprofen and anesthetized using 2% isoflurane in air. The animal is then secured in a stereotaxic frame (ASlinstruments, Inc.) and a hole is burred 2 mm right lateral and 1 mm anterior to the coronal suture. The cell suspension (kept on wet ice) is thoroughly mixed and drawn up into a 50 μL syringe. The syringe needle is placed in the center of the burr hole and pushed down 3 mm into the brain and pulled back 1 mm to form a "reservoir" of cell suspension sediment. Then 10 μL of cell suspension (1 × 10 per mouse) is added. 6 Cells) are gradually injected into the brain tissue. Tumor progression is followed by in vivo bioluminescence imaging performed using an IVIS 50 optical imaging system (Xenogen, Alameda, CA). Bioluminescence images are acquired at regular intervals for tumor burden estimation. The amount of light emitted from the tumor after systemic injection of D-luciferin is expected to correlate with tumor size. Each mouse is injected subcutaneously (IP) with 150 mg / kg D-luciferin and imaged 10 min after injection. Small and medium binning of the CCD chip is used and exposure times are adjusted (10 s to 1 min) to obtain at least several hundred counts from the tumor to avoid saturating the CCD chip. Images are analyzed using Living Image (Xenogen, Alameda, CA) software. Each unique signal is manually circled and labeled by group and mouse number. The average brain bioluminescence signal across all groups in the experiment was 280 × 10 6 When the IL-16 signal is detected, treatment is initiated by oral gavage of the test compound. All procedures performed in this experiment are in compliance with all laws, regulations, and guidelines of the National Institutes of Health (NIH).

[0244] At the end of the study, mice are euthanized by carbon dioxide overexposure for blood and brain collection. Whole blood is collected by terminal cardiac puncture and placed into EDTA Microtainer® tubes. Brains are excised and placed in 10% neutral buffered formalin. MDA-MB-231 xenograft study

[0245] To evaluate the in vivo anticancer activity of the compounds of formula I, MDA-MB-231-luc-D3H2LN human breast cancer xenografts are used. Briefly, MDA-MB-231-luc-D3H2LN cells are obtained from Xenogen and grown in Minimum Essential Medium (MEM) containing EBSS modified with 1% L-glutamine and supplemented with 10% FBS, 1% PSG, 1% non-essential amino acids, and 1% sodium pyruvate. The growth environment is maintained at 37°C in an incubator under a 5% CO2 atmosphere. Cells are harvested and resuspended using 50% serum-free medium and 50% Matrigel®, and 5×10 6 Generate a stock concentration of cells / mL.

[0246] Six to seven week old female CB-17 / IcrHsd-PrkdcscidLystbg mice are injected subcutaneously just under the right axilla with 200 μL of cell suspension. All procedures performed in this experiment are in compliance with all National Institutes of Health (NIH) laws, regulations, and guidelines. Treatment begins when the mean tumor burden is approximately 150 mg. All mice are administered test compounds by oral gavage. Body weights and tumor measurements are recorded three times weekly. Tumor burden (mg) is estimated from caliper measurements by the following equation for the volume of a prolate spheroid that estimates unit density: Tumor burden (mg) = (L × W2) / 2, where L and W are the respective orthogonal tumor length and width measurements (mm). The primary endpoint to evaluate efficacy is %T / C. %T / C is defined as the median tumor mass of the treatment group divided by the median tumor mass of the control group multiplied by 100. In vitro bioluminescence imaging is performed using an IVIS 50 optical imaging system (Xenogen, Alameda, CA) when animals leave the study. Animals are injected intraperitoneally with 150 mg / kg D-luciferin (Promega) and euthanized 10 min after injection. Primary tumors are removed and flash frozen for further analysis, and mice are opened and imaged in supine position. Large binning of the CCD chip is used and exposure times are adjusted (1-2 min) to obtain at least several hundred counts from the tumor to avoid saturation of the CCD chip. Images are analyzed using Living Image (Xenogen, Alameda, CA) software. Each unique signal is manually circled and labeled by group and mouse number. Total BLI signal is correlated with tumor size and compared to vehicle control to determine treatment benefit.

[0247] Certain 2-aminopyrimidin-6-ones have been reported in WO 2008 / 079291 to be inhibitors of VEGFR / KDR and / or c-MET kinases, and are shown below in Figure 1. Evidence of kinase inhibition was observed with K values ​​of 6-87 nM (as shown in Figure 1). iwas reported for certain inhibitors vs. cMET kinase in WO 2008 / 079291. No information on the inhibition of c-FMS kinase was disclosed in WO 2008 / 079291. These compounds of WO 2008 / 079291 differ from the compounds of the present invention by the presence of the arylamino "A" moiety of formula I of the present invention, where A is NR2(R3), R2 is aryl and R3 is H.

[0248] These compounds of WO 2008 / 079291 are outside the scope of the present invention. Nevertheless, the compounds of the present invention were screened against both c-MET and KDR kinases. Unexpectedly, the compounds of the present invention were found to confer a high level of selectivity for c-FMS kinase versus c-MET and KDR kinases. The most potent compound of formula I in the c-MET assay had an IC of 3.4 micromolar versus 0.0016 micromolar in the u-FMS assay. 50 The most potent compound of formula I in the KDR assay had an IC of 1.4 micromolar compared to 0.0016 micromolar in the u-FMS assay, demonstrating selectivity of 2125-fold. 50 The results showed a selectivity of 875-fold, with A being a non-aromatic moiety. These data demonstrate that the compounds of the present invention (wherein A is a non-aromatic moiety) potently inhibit c-FMS kinase, but do not readily inhibit cMET and KDR kinase activity. These results could not have been anticipated by the prior teachings of WO 2008 / 079291. [ka] Figure 1. cMET inhibitors from WO 2008 / 079291. equivalent

[0249] Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments specifically described in this disclosure which equivalents are intended to be encompassed within the scope of the following claims.

Claims

1. Formula 26 【Chemistry 1】 A compound of the formula: During the ceremony, W is a 5- or 6-membered heteroaryl, phenyl, -NHC(O)R6, -NHC(O)R7, -NHC(O)N(R8)R9, or -C(O)N(R8)R9, each 5- or 6-membered heteroaryl or phenyl optionally substituted by 1, 2, or 3 R5 moieties; X1 is hydrogen and X2 is hydrogen or C1-C6 alkyl; Each R5 is independently selected from hydrogen, C1-C6 alkyl, deuterated -C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, branched C3-C8 alkyl, halogen, cyano, fluoro-C1-C6 alkyl in which the alkyl chain is partially or fully fluorinated, -(CH ２ ) ｍ -C(O)NR8(R9), -(CH2)m-C(O)R7, -(CH2)m-OR8, -(CH2)m-NR8(R9), or -(CH2)m-R7, each alkylene optionally substituted with C1-C4 alkyl; each R6 is independently halogen, C1-C6 alkyl, branched C3-C8 alkyl, C3-C8 cycloalkyl, -(CH2)m-CN, -(CH2)m-OR8, -(CH2)m-NR8(R9), or -(CH2)m-R7, each alkylene optionally substituted with C1-C4 alkyl; Each R7 is independently 【Chemistry 2】 wherein the symbol (##) is the point of attachment to the W, R5, or R6 moiety, respectively, containing the R7 moiety; Each R7 is -(R10) ｐ optionally replaced by each R8 and R9 is independently hydrogen, C1-C6 alkyl, fluoro-C1-C6 alkyl in which the alkyl chain is partially or fully fluorinated, or branched C3-C8 alkyl; each R10 is independently a C1-C6 alkyl, -(CH2)m-CN, -(CH2)m-OR3, or -(CH2)m-NR8(R9), each alkyl or alkylene optionally substituted with one or two C1-C6 alkyl; each alkylene is optionally substituted with C1-C4 alkyl; R3 is hydrogen, C1-C6 alkyl, deuterated-C1-C6 alkyl in which the alkyl chain is partially or fully deuterated, branched C3-C8 alkyl, C3-C8 cycloalkyl, fluoro-C1-C6 alkyl in which the alkyl is fully or partially fluorinated, or a 3- to 8-membered heterocyclic ring; each m is independently 0, 1, 2, or 3; The compound wherein each p is 0, 1, 2, or 3.

2. The compound has the following structure: 【Chemistry 3】 2. The compound of claim 1 having the formula:

3. 2. A process for preparing a compound of formula 26 according to claim 1, said process comprising the step of: 【Chemistry 4】 The compound of formula 6 【Chemistry 5】 to produce a compound of formula 26, During the ceremony, W, X1, and X2 are as defined for the compound of formula 26 in claim 1; The process wherein M is selected from the group consisting of H, trialkylstannyl, boronic acid, and boronic ester, with the proviso that when M is H, W is -NHC(O)R6, -NHC(O)R7, or -NHC(O)N(R8)R9.

4. Formula 24 【Chemistry 6】 4. The process of claim 3, further comprising reducing the compound of formula 25 to produce a compound of formula 25, wherein X1 and X2 are as defined for the compound of formula 26 in claim 1.

5. 5. The process of claim 4, wherein said reducing comprises the presence of zinc and ammonium chloride.

6. 6. The process of claim 4 or 5, further comprising the presence of a protic solvent.

7. 26. A process for preparing a compound of formula 26 according to claim 1, said process comprising: (i) Formula 24 【Chemistry 7】 The compound of formula 6 【Chemistry 8】 to produce an intermediate compound containing a nitro moiety; During the ceremony, W, X1, and X2 are as defined for the compound of formula 26 in claim 1; M is selected from the group consisting of H, trialkylstannyl, boronic acid, and boronic ester, with the proviso that when M is H, W is -NHC(O)R6, -NHC(O)R7, or -NHC(O)N(R8)R9; and (ii) reducing said intermediate compound containing a nitro moiety to produce a compound of formula 26.

8. 8. The process of claim 7, wherein said reducing comprises the presence of zinc and ammonium chloride.

9. 9. The process of claim 7 or 8, further comprising the presence of a protic solvent.

10. Formula 22 【Chemistry 9】 The compound of formula 23 【Chemistry 10】 to produce a compound of formula 24, During the ceremony, X1 and X2 are as defined for compounds of formula 26 in claim 1, The process of claim 4 wherein Y is a halogen.

11. 11. The process of claim 10 further comprising the presence of a base.

12. The compound of formula 26 has the structure 【Chemistry 11】 8. The process of claim 3 or 7, comprising:

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