Composition for improving blood flow and composition for improving cold sensitivity

Abstract

To provide a composition that promotes peripheral blood flow and exhibits excellent efficacy in improving cold sensitivity.SOLUTION: A composition for improving cold sensitivity comprises jujube seeds and Perilla herb as active ingredients.SELECTED DRAWING: Figure 3

Description

[Technical field]

[0001] The present invention relates to a composition for improving blood flow and a composition having an effect of improving sensitivity to chills. [Background technology]

[0002] Sensitivity to cold is one of the complaints caused by autonomic nervous system imbalance, and is a symptom seen in a wide range of age groups, from young people to the elderly. However, it is particularly common in women, with one in two women said to suffer from sensitivity to cold. It is believed that one of the main causes of cold is a decrease in autonomic nervous function due to stress or an irregular lifestyle, which causes peripheral blood vessels to constrict and reduce blood flow. Cold can lead to organic symptoms such as headaches and back pain, as well as systemic symptoms such as fatigue and a weakened immune system.

[0003] Various studies have been conducted so far on ingredients that are effective in improving sensitivity to cold. For example, it has been reported that cinnamon extract (Patent Document 1), black ginger rhizome extract (Patent Document 2), amla fruit (Patent Document 3), and extract of pepper longum (Patent Document 4) are effective in promoting peripheral blood flow and improving sensitivity to cold.

[0004] However, there is still a need for a component that can provide sufficient effectiveness in improving chills. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] JP 2009-106245 A [Patent Document 2] JP 2009-67731 A [Patent Document 3] JP 2006-335713 A [Patent Document 4] Patent No. 448266 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present invention is to provide a composition that promotes peripheral blood flow and has an excellent effect of improving sensitivity to chills. [Means for solving the problem]

[0007] As a result of intensive research to solve the above-mentioned problems, the inventors discovered that the combination of Zizyphus jujube seed and Perilla frutescens exhibits a synergistic effect of improving blood flow, and that ingesting this combination provides an excellent effect of improving chills, thus completing the present invention.

[0008] That is, the present invention is a composition for improving chills, which contains Zizyphus oleifera and Perilla frutescens as active ingredients.

[0009] The present invention also relates to a composition for improving blood flow, which contains Zizyphus oleifera and Perilla frutescens as active ingredients. Effect of the Invention

[0010] When ingested, the composition of the present invention has a synergistic blood flow promoting effect, thereby providing an excellent effect of improving sensitivity to chills. [Brief description of the drawings]

[0011] [Figure 1] 1 is a graph showing the adenosine A2A receptor agonist activity by administration of Zizyphus jujube seed alone in the GCPR assay of Test Example 1. [Diagram 2] 1 is a graph showing the adenosine A2A receptor agonist activity by administration of Perilla frutescens alone in the GCPR assay of Test Example 1. [Diagram 3] 1 is a graph showing the adenosine A2A receptor agonist activity of a combination of Zizyphia juncea and Perilla frutescens in the GCPR assay of Test Example 1. [Figure 4] 1 is a graph showing the results of evaluation by VAS regarding cold fingers in Test Example 2. [Diagram 5] 1 is a graph showing the results of evaluation by VAS regarding cold feet in Test Example 2. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] The composition of the present invention uses Zizyphus jujube seed and Perilla frutescens leaf as active ingredients.

[0013] Jujube kernel is a herbal medicine originating from the seeds of Ziziphus jujuba Miller var. spinosa Hu ex HF Chow, a member of the Rhamnaceae family. The jujube kernel used in the present invention may be the herbal medicine as it is, or may be a herbal powder obtained by shredding or crushing the herbal medicine, or a herbal extract obtained by extracting the herbal medicine with a solvent such as water or ethanol. The herbal extract may be a concentrated soft extract or a dried extract powder.

[0014] Perilla frutescens Britton var. crispa W. Deane is a herbal medicine originating from the dried leaves of Perilla frutescens Britton var. crispa W. Deane of the Lamiaceae family. The Perilla frutescens used in the present invention may be the herbal medicine as it is, or may be a powder of the herbal medicine obtained by shredding or crushing the herbal medicine, or a herbal medicine extract obtained by extracting the herbal medicine with a solvent such as water or ethanol. The herbal medicine extract may be a concentrated soft extract or a dried extract powder.

[0015] The mass ratio of Zizyphimurium jujube seed to Perilla frutescens leaf in the composition of the present invention (in the case of a herbal extract, the weight equivalent to the herbal medicine) is not particularly limited, but from the viewpoint of improving blood flow and improving chills, it is preferably 10:1 to 10.55:1 (Zizyphimurium jujube seed:Perilla frutescens leaf), and more preferably 10.13:1 to 10.42:1.

[0016] Adenosine receptor (A 1 , A 2A , A 2B , A 3 ) is widely distributed in the body and has a variety of physiological functions. 2A It is known that when the receptor is activated, it causes dilation of coronary and peripheral arteries and increases blood flow in cerebral capillaries. 2AReceptor agonist activity, but when used in combination, it has a synergistic adenosine A 2A It exhibits receptor agonist activity, thereby expanding peripheral blood vessels to promote blood flow, and can obtain an excellent effect of improving cold. The use of the composition of the present invention includes use for improving blood flow and use for improving cold. The site for improving blood flow is not particularly limited, and examples include the extremities such as fingers and toes, the upper arm, the thigh, the brain, etc., but it is preferably used for cold extremities such as fingers and toes. In the present invention, the above-mentioned use for improvement also includes its preventive use.

[0017] The composition of the present invention includes human or animal medicines, quasi-drugs, foods, feeds, etc. Examples of dosage forms of medicines and quasi-drugs include tablets, capsules, granules, powders, chewable tablets, syrups, etc. In preparing these preparations, in addition to the active ingredients Jujube kernel and Perilla herb, pharmacopoeias, pharmacopoeias, etc., can be appropriately combined with pharmacopoeias, ...

[0018] The dosage of the composition of the present invention is not particularly limited and can be set as appropriate; for example, the daily dosage for an adult is approximately 1.7 to 2.0 g of jujube seed and 0.16 to 0.20 g of perilla leaf extract (converted into the weight of herbs in the case of herbal extracts).

[0019] When the composition of the present invention is used as a food, the form of the food may be jellies, hard candies, soft candies, gummies, gums, wafers, biscuits, chocolates, breads, noodles, sausages, and other foods, nutritional foods, and other foods, as well as nutritional supplement compositions in the same form as the above-mentioned medicines (tablets, capsules, chewable tablets, syrups, etc.). In preparing these foods, in addition to the active ingredients of sour jujube seeds and perilla leaves, other food materials, sweeteners, gelling agents, colorants, fungicides, acidulants, seasonings, pH adjusters, leavening agents, solvents, softeners, oils, emulsifiers, preservatives, aromatics, stabilizers, colorants, antioxidants, moisturizers, thickeners, etc. may be appropriately combined and produced according to conventional methods. Foods include functional foods, foods for specified health uses, and foods with functional claims.

[0020] The composition of the present invention may be labeled with, for example, the following: preventing or improving sensitivity to cold, improving sensitivity to cold, improving blood flow to maintain body temperature, expanding blood vessels to maintain blood flow, promoting blood circulation. The act of labeling includes indicating the product itself, container, packaging, etc., containing the composition of the present invention, indicating the product in documents such as instructions, package inserts, pamphlets, and other printed materials disclosing product information, indicating the product in various leaflets, advertisements used for promotion including the Internet, etc.

[0021] The present invention will be described in more detail below with reference to examples and test examples, but the present invention is not limited to these examples. EXAMPLES

[0022] Test Example 1 Adenosine A 2A Receptor agonist activity testing; Reporter cells were cultured in DMEM supplemented with 10% FCS and penicillin-streptomycin. Cells were released using trypsin-EDTA and suspended in DMEM containing 10% dialyzed FCS. The suspended cells were seeded at 24,000 cells per well in a 96-well white microplate. The cells were incubated in 5% CO2 for 30 min to allow the seeded cells to adhere to the bottom of the microplate. 2The mixture was incubated overnight at 37°C in the presence of Plasmids expressing GPCR, Gα protein, and luciferase reporter were mixed and transfected at a total of 100 ng per well using a transfection reagent (FuGENE6, Promega). 2 The mixture was incubated overnight at 37°C in the presence of 2 ml of DMSO was added to 180 mg each of sour jujube seed, soybean leaf, and mixed powder of sour jujube seed and soybean leaf, and the solution was homogenized by ultrasonic treatment. After centrifugation (10,000 rpm, 10 minutes), the supernatant was collected and filtered through a filter (0.45 μm) to prepare the herbal extract. The herbal extracts were diluted to 7-fold the final test concentration using DMEM containing 10% dialyzed FCS and added to each well. After addition, the wells were incubated in 2.5% CO 2 The cells were cultured in the presence of 5% CO at 37° C. for 6 hours. After aspirating the medium, a luciferase quantification reagent (Steady-Glo, Promega) was added to the wells, and the amount of luciferase luminescence was measured using a plate reader (VictorNivo, PerkinElmer). Data analysis was performed by approximating the Hill equation using the nonlinear least squares method. The parameters were EC50, Hill coefficient, curve bottom, and curve top. The results are shown in Table 1 and Figures 1 to 3.

[0023]

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[0024] [Table 1]

[0025] These results confirmed that the combination of Zizyphus jujube seed and Perilla frutescens increased the Hill coefficient, an indicator of reactivity, by approximately 80% compared to using either ingredient alone, demonstrating a synergistic effect.

[0026] Example 1 Manufacturing of tablets containing jujube seeds and perilla leaves The cut herbal medicines Jujube kernel and Perilla herb were added with 10 times the amount of purified water, and the mixture was heated to 95-100°C with stirring, and then extracted for about 60 minutes. After the extraction was completed, the extract was separated into solid and liquid, and the separated liquid was concentrated under reduced pressure and then spray-dried to obtain dried Jujube kernel and Perilla herb extract powder. The obtained jujube seed and perilla leaf extract powder were mixed with lactose, microcrystalline cellulose, and magnesium stearate, and pressed into tablets using a tablet press to produce tablets containing 1.85 g of jujube seed in terms of herbal medicine weight and 0.18 g of perilla leaf in terms of herbal medicine weight per three tablets (containing a total of 52 mg of jujube seed extract powder and perilla leaf extract powder).

[0027] Test Example 2 Human trials The subjects were healthy women aged 35 to 50 years, 30 in each group. They were asked to take the tablets prepared in Example 1 once a day for 12 weeks, and 4, 8, and 12 weeks after the start of intake, "cold fingers" and "cold feet" were evaluated using a VAS (Visual Analogue Scale) (herbal medicine group). As a control, they were asked to take tablets that did not contain Zizyphi jujube kernel and Perilla oleracea extract powder, and similarly evaluated using a VAS (control group). The results are shown in Figures 4 and 5.

[0028] As shown by these results, it was confirmed that the combined use of Zizyphi jujube seed and Perilla frutescens significantly improved coldness in the extremities, such as the hands, feet and toes, and that this effect increased over time. [Industrial Applicability]

[0029] The composition of the present invention, when ingested, has the effect of promoting peripheral blood flow and alleviating chills, and is useful as a medicine, food, etc. for alleviating chills.

Claims

1. A composition for improving chills, containing jujube seed and perilla leaf as active ingredients.

2. 2. The composition for improving chills according to claim 1, wherein the chills are chills in the fingers or toes.

3. A composition for improving blood flow containing Zizyphus jujube seed and Perilla frutescens as active ingredients.

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