Fumaryl diketopiperidine prodrug of treprostinil

Abstract

To provide novel treprostinil-based compounds, therapeutic methods using them, and methods for their production.SOLUTION: For example, a compound of the formula 5a in the figure or a pharmaceutically acceptable salt thereof is provided. (In the formula, R5 is H.)SELECTED DRAWING: None

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority to U.S. Provisional Application No. 63 / 036,561, filed June 9, 2020, which is incorporated by reference in its entirety.

[0002] Technical Field This application relates generally to prostacyclins, and more specifically to prodrugs of treprostinil, and methods of making and using such prodrugs. Summary of the Invention

[0003] summary In one embodiment, R 5 is H or a polymeric carrier, or a pharma- ceutically acceptable salt thereof. [ka] [ka]

[0004] Another embodiment is a pharma- ceutically acceptable batch comprising a compound of the preceding embodiments having a purity of at least 90%.Another embodiment is a pharmaceutical composition comprising a compound of the preceding embodiments.

[0005] Another embodiment is a method for making an FDKP-Treprostinil compound comprising: i) Treprostinil (1) [ka] The doubly protected treprostinil moiety (2) [ka] (wherein R 1is a carboxylic acid protecting group, and 2 is H and R 3 is a hydroxyl protecting group, or b) R 2 is a hydroxyl protecting group, R 3 is H); ii) Recombining a doubly protected treprostinil moiety with a compound of formula (3) [ka] (In the formula, R 4 is H) to produce a dual-protected FDKP-Treprostinil compound having formula (4a) or (4b); and [ka] [ka] iii) deprotecting the doubly protected FDKP-Treprostinil compound to produce an FDKP-Treprostinil compound having formula (5a) or (5b). [ka] [ka]

[0006] Another embodiment is a method of treating a treprostinil-treatable condition, comprising administering to a subject in need thereof a compound of the preceding embodiment. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0007] Detailed Description As used in the specification and claims, the singular forms "a," "an," and "the" include plural references unless the context clearly dictates otherwise. Throughout this specification, unless otherwise indicated, "comprise," "comprises," and "comprising" are used inclusively rather than exclusively. The term "or" is inclusive unless modified, for example, by "either." Thus, unless the context or clear statement dictates otherwise, the word "or" means any one member of a particular list and also includes any combination of members of that list. Except in the examples or where otherwise indicated, all numbers expressing quantities of ingredients or reaction conditions used herein should be understood to be modified in all instances by the term "about."

[0008] Headings are provided for convenience only and should not be construed as limiting the invention in any way. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used herein is for the purpose of describing certain embodiments only and is not intended to limit the scope of the invention, which is defined solely by the claims. In order to make this disclosure more readily understandable, certain terms are defined first. Additional definitions, all numerical designations, e.g., pH, temperature, time, concentration, molecular weight, including ranges, are approximate and vary (+) or (-) by increments of 1, 5, or 10%. It should be understood, although not always explicitly stated, that all numerical designations are preceded by the term "about". It should also be understood, although not always explicitly stated, that the reagents described herein are merely exemplary, equivalents of which are known in the art and are described throughout the detailed description.

[0009] "HPLC" refers to high performance liquid chromatography.

[0010] "NMR" refers to nuclear magnetic resonance.

[0011] "FDKP" refers to fumaryl 2,5-diketopiperazine or (E)-3,6-bis[4-(N-carbonyl-2-propenyl)amidobutyl]-2,5-diketopiperazine.

[0012] "RRT" refers to relative retention time.

[0013] "TMSE" refers to trimethylsilyl ethyl ester.

[0014] "TMBDS" refers to tert-butyldimethylsilyl.

[0015] "EDCI" refers to 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide.

[0016] "DMAP" refers to 4-dimethylaminopyridine.

[0017] "DMA" refers to N,N-dimethylacetamide;

[0018] "DMF" refers to N,N-dimethylformamide.

[0019] "TBAF" refers to tetra-n-butylammonium fluoride.

[0020] "THF" refers to tetrahydrofuran.

[0021] "LCMS" refers to liquid chromatography mass spectrometry.

[0022] "IR" refers to infrared spectroscopy.

[0023] "TFA" refers to trifluoroacetic acid.

[0024] "DIEA" or "DIPEA" refers to N,N-diisopropylethylamine.

[0025] "DQF-COSY" refers to double quantum filter correlation spectroscopy.

[0026] "ACN" refers to acetonitrile.

[0027] "HOBt" refers to hydroxybenzotriazole.

[0028] "DEPT-NMR" refers to distortion-free enhancement by polarization transfer nuclear magnetic resonance.

[0029] As used herein, "protecting groups" or "protective groups" are known in the art and are set forth in TW Green, PGM Wuts, Protective Groups in Organic Synthesis, Wiley-Interscience, New York, 1999 (hereinafter "Greene, Protective Groups in Organic Synthesis"), which is incorporated herein by reference in its entirety for its teachings regarding protecting groups.

[0030] As used herein, "hydroxyl protecting group" or "hydroxyl protective group" refers to the commonly understood definition of an alcohol or hydroxyl protecting group as defined in TW Green, PGM Wuts, Protective Groups in Organic Synthesis, Wiley-Interscience, New York, 1999.

[0031] As used herein, "carboxylic acid protecting group", "carboxyl protecting group", "carboxylic acid protective group", "carboxyl protective group" refer to the commonly understood definition of a carboxyl protecting group as defined in TW Green, PGM Wuts, Protective Groups in Organic Synthesis, Wiley-Interscience, New York, 1999.

[0032] As used herein, C m ~C n , for example C 1 ~C 12 , C 1 ~C 8 , or C 1 ~C 6 when used before a group refers to a group containing from m to n carbon atoms.

[0033] "Optionally substituted" refers to a group selected from the group and the substitution forms of that group. The substituents may include any of the groups defined below. In one embodiment, the substituents are C 1 ~C 10 Or C 1 ~C 6 Alkyl, substituted C 1 ~C 10 Or C 1 ~C 6 Alkyl, C 2 ~C 6 Alkenyl, C 2 ~C 6 Alkynyl, C 6 ~C 10 Aryl, C 3 ~C 8 Cycloalkyl, C 2 ~C 10 Heterocyclyl, C 1 ~C 10 Heteroaryl, substituted C2 ~C 6 Alkenyl, substituted C 2 ~C 6 Alkynyl, Substituted C 6 ~C 10 Aryl, Substituted C 3 ~C 8 Cycloalkyl, substituted C 2 ~C 10 Heterocyclyl, substituted C 1 ~C 10 Heteroaryl, halo, nitro, cyano, -CO 2 H or its C 1 ~C 6 The alkyl esters are selected from the group consisting of alkyl esters.

[0034] "Pharmaceutically acceptable salt" refers to a salt of a compound, which is suitable for pharmaceutical use and derived from various organic and inorganic counterions well known in the art. Pharmaceutically acceptable salts include, by way of example only, sodium, potassium, calcium, magnesium, ammonium, and tetraalkylammonium when the compound contains an acidic functional group. When the molecule contains a basic functional group, it includes salts of organic or inorganic acids, such as hydrochloride, hydrobromide, tartrate, mesylate, acetate, maleate, and oxalate. Stahl and Wermuth, eds., "Handbook of Pharmaceutically Acceptable Salts," (2002), Verlag Helvetica Chimica Acta, Zurich, Switzerland, which is incorporated herein by reference for its teachings on pharmaceutically acceptable salts, discusses various pharmaceutical salts, their selection, preparation, and use.

[0035] "Pulmonary hypertension" refers to all forms of pulmonary hypertension, WHO Groups 1 to 5. Pulmonary arterial hypertension, also called PAH, refers to WHO Group 1 pulmonary hypertension. PAH includes idiopathic, hereditary, drug- or toxin-induced, and persistent pulmonary hypertension of the newborn (PPHN).

[0036] In general, a pharma- ceutically acceptable salt is a salt that substantially retains one or more of the desired pharmacological activities of the parent compound and is suitable for in vivo administration. Pharmaceutically acceptable salts include acid addition salts formed with inorganic or organic acids. Inorganic acids suitable for forming pharma- ceutically acceptable acid addition salts include, by way of example and not limitation, hydrohalic acids (e.g., hydrochloric acid, hydrobromic acid, hydroiodic acid, etc.), sulfuric acid, nitric acid, phosphoric acid, etc.

[0037] Organic acids suitable for forming pharma- ceutically acceptable acid addition salts include, by way of example and not limitation, acetic acid, trifluoroacetic acid, propionic acid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, oxalic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, palmitic acid, benzoic acid, 3-(4-hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid, alkylsulfonic acids (e.g., methanesulfonic acid, ethanesulfonic acid, 1,2-ethanedisulfonic acid, 2-hydroxyethanesulfonic acid, and the like), arylsulfonic acids (e.g., benzenesulfonic acid, 4-chlorobenzenesulfonic acid, 2-naphthalenesulfonic acid, 4-toluenesulfonic acid, camphorsulfonic acid, and the like), glutamic acid, hydroxynaphthoic acid, salicylic acid, stearic acid, muconic acid, and the like.

[0038] Pharmaceutically acceptable salts also include salts formed when an acidic proton present in the parent compound is replaced by a metal ion (e.g., an alkali metal ion, an alkaline earth metal ion, or an aluminum ion) or an ammonium ion (e.g., the ammonium ion from organic bases such as ethanolamine, diethanolamine, triethanolamine, morpholine, piperidine, dimethylamine, diethylamine, triethylamine, and ammonia).

[0039] Treprostinil, the active ingredient in Remodulin® (treprostinil) injection, Tyvaso® (treprostinil) inhalation solution, and Orenitram® (treprostinil) sustained release tablets, was described in U.S. Patent No. 4,306,075. Methods for preparing treprostinil and other prostacyclin derivatives are described, for example, in Moriarty, et al., J. Org. Chem. 2004, 69, 1890-1902, Drug of the Future, 2001, 26(4), 364-374, Nos. 6,441,245, 6,528,688, 6,700,025, 6,809,223, 6,756,117, 8,461,393, 8,481,782; 8,242,305, 8,497,393, 8,940,930, 9,029,607, 9,156,786, and 9,388,154, 9,346,738; U.S. Patent Application Publication Nos. 2003-2009022 and 2003-2006022. Nos. 012-0197041, 2013-0331593, 2014-0024856, 2015-0299091, 2015-0376106, 2016-0107973, 2015-0315114, 2016-0152548, and 2016-0175319; PCT Publication Nos. WO2016 / 0055819 and WO2016 / 081658.

[0040] Various uses and / or various forms of treprostinil are described, for example, in U.S. Pat. Nos. 5,153,222, 5,234,953, 6,521,212, 6,756,033, 6,803,386, 7,199,157, 6,054,486, 7,417,070, 7,384,978, 7,879,909, 8,563,614, 8,252,839, 8,536,363, 8,410,169, 8, No. 232,316, No. 8,609,728, No. 8,350,079, No. 8,349,892, No. 7,999,007, No. 8,658,694, No. 8,653,137, No. 9,029,607, No. 8,765,813 No. 9,050,311, 9,199,908, 9,278,901, 8,747,897, 9,358,240, 9,339,507, 9,255,064, 9,278,902, 9,278 ,903, 9,758,465; 9,422,223; 9,878,972; 9,624,156; U.S. Patent Application Publication Nos. 2009-0036465, 2008-0200449, 2008-0280986, 2009-0124697, 2014-0275616, 2014-0275262, 2013-0184295, 2014-0323567, 2016-0030371, 2016- 0051505, 2016-0030355, 2016-0143868, 2015-0328232, 2015-0148414, 2016-0045470, 2016-0129087, 2017-0095432; 2018-0153847, and PCT Publication Nos. WO00 / 57701, WO20160105538, WO2016038532, WO2018 / 058124.

[0041] Treprostinil has the following chemical formula:

[0042] [ka]

[0043] The present inventors have discovered a compound of formula (5a) or (5b): [ka] [ka] or a pharma- ceutical acceptable salt thereof. In formula (5a) and (5b), R 5 is H or a polymeric carrier, such as a polyethylene glycol (PEG) carrier, a fatty alcohol carrier, or a fatty amine carrier.

[0044] In some embodiments, the polymeric carrier, such as a PEG carrier, can have an average molecular weight, such as a number average molecular weight, of about 200 to about 200,000. In some embodiments, the polymeric carrier, such as a PEG carrier, can have an average molecular weight, such as a number average molecular weight, of about 200 to about 80,000. In some embodiments, the polymeric carrier can be PEG 1500, PEG 4000, PEG 5000, PEG 8000, PEG 10,000, PEG 15,000, PEG 20,000, and PEG 25,000. In some embodiments, the polymeric carrier can be PEG 20,000.

[0045] In some embodiments, the fatty alcohol carrier is 1 ~C 20The fatty alcohol carrier may include alcohol. In some embodiments, the fatty alcohol carrier is saturated or unsaturated. In some embodiments, the fatty alcohol carrier is a saturated fatty alcohol carrier, such as 1-hexanol, 1-heptanol, 1-octanol, 1-nonanol, 1-decanol, etc. In some embodiments, the fatty alcohol carrier is an unsaturated fatty alcohol carrier, such as 10-undecen-1-ol, (Z)-9-octadecen-1-ol, (E)-9-octadecen-1-ol, (Z,Z)-9,12-octadecadien-1-ol, etc. Other fatty alcohols known in the art can be used, for example, those disclosed in K. Nowek et al., (2012) 'Fatty Alcohols' in Ullmann's Encyclopedia of Industrial Chemistry. Wiley-VCH Verlag GmbH & Co. KGaA, which is incorporated herein in its entirety for its teaching of fatty alcohols.

[0046] In some embodiments, R 5 is a fatty amine carrier that forms an amide bond with a carboxyl group. In some embodiments, the fatty amine carrier is 1 ~C 20amine. In some embodiments, the fatty amine carrier is saturated or unsaturated. In some embodiments, the fatty amine carrier is a saturated fatty amine carrier, such as 1-hexaneamine, 1-heptanamine, 1-octanamine, 1-nonanamine, 1-decaneamine, etc. In some embodiments, the fatty amine carrier is an unsaturated fatty amine carrier, such as 10-undecene-1-amine, (Z)-9-octadecene-1-10-undecene-1-amine, (E)-9-octadecene-1-10-undecene-1-amine, (Z,Z)-9,12-octadecadien-1-10-undecene-1-amine, etc. Other fatty amines known in the art can be used as fatty amine carriers, for example, those disclosed in K. Nowek et al., (2012) 'Fatty Alcohols' in Ullmann's Encyclopedia of Industrial Chemistry. Wiley-VCH Verlag GmbH & Co. KGaA, which is incorporated herein in its entirety for its teachings on fatty alcohols.

[0047] When administered to a subject, such as a human, the prodrug undergoes in vivo biotransformation, such as chemical or enzymatic cleavage, to enable delivery of an effective amount of Treprostinil to the subject.

[0048] Pharmaceutical Compositions The treprostinil prodrug may be provided in the form of a pharmaceutical composition, which may also include pharma- ceutically acceptable carriers, excipients, binders, diluents, and the like. Such pharmaceutical compositions may be prepared by methods known in the art, such as granulation, mixing, dissolving, encapsulation, lyophilization, emulsification, or wet-grinding processes, among others. The compositions may be in the form of, for example, granules, powders, tablets, capsules, syrups, suppositories, injections, emulsions, elixirs, suspensions, and solutions. The compositions may be formulated for many different routes of administration, for example, oral, transmucosal, rectal, transdermal, or subcutaneous administration, as well as intrathecal, intravenous, intramuscular, intraperitoneal, intranasal, intraocular, or intraventricular injection. The treprostinil prodrug may be administered by any of the above routes, for example, locally rather than systemically, as an injection or sustained-release formulation.

[0049] In some embodiments, the treprostinil prodrug can be administered by inhalation. Treprostinil can be formulated for dry powder inhalation (DPI) using any suitable technique or formulation.

[0050] In one embodiment, the pharmaceutical composition can be made by mixing the treprostinil prodrug with a carrier such as sterile water. In some embodiments, the treprostinil prodrug is formulated for subcutaneous administration, and such formulations may or may not contain m-cresol or another preservative.

[0051] For oral, buccal and sublingual administration, powders, suspensions, granules, tablets, pills, capsules, gelcaps and caplets may be acceptable solid dosage forms. These may be prepared, for example, by mixing one or more treprostinil prodrugs or their pharma- ceutically acceptable salts with at least one additive or excipient, such as starch or other additives. Suitable additives or excipients may be sucrose, lactose, cellulose sugar, mannitol, maltitol, dextran, sorbitol, starch, agar, alginate, chitin, chitosan, pectin, tragacanth gum, gum arabic, gelatin, collagen, casein, albumin, synthetic or semi-synthetic polymers or glycerides, methylcellulose, hydroxypropylmethylcellulose, and / or polyvinylpyrrolidone. Optionally, the oral dosage form may contain other ingredients that aid in administration, such as inert diluents or lubricants, such as magnesium stearate, preservatives, such as parabens and sorbic acid, antioxidants, such as ascorbic acid, tocopherol, or cysteine, disintegrants, binders, thickeners, buffers, sweeteners, flavorings, or fragrances. In addition, dyes or pigments may be added for identification. Tablets may be further treated with suitable coating materials known in the art.

[0052] Liquid dosage forms for oral administration include: The pharmaceutical compositions may be in the form of pharma- ceutically acceptable emulsions, syrups, elixirs, suspensions, slurries, and solutions, which may include inert diluents such as water. Pharmaceutical formulations may be prepared as liquid suspensions or solutions using sterile liquids, including, but not limited to, oils, water, alcohols, and combinations thereof. Pharmaceutically suitable surfactants, suspending agents, and emulsifying agents may be added for oral or parenteral administration.

[0053] As mentioned above, the suspension may contain oil. Such oils include, but are not limited to, peanut oil, sesame oil, cottonseed oil, corn oil, and olive oil. The suspension preparation may also contain esters of fatty acids, such as ethyl oleate, isopropyl myristate, fatty acid glycerides, and acetylated fatty acid glycerides. The suspension preparation may also contain alcohol, such as, but are not limited to, ethanol, isopropyl alcohol, hexadecyl alcohol, glycerol, and propylene glycol. Ethers, such as, but are not limited to, poly(ethylene glycol), petroleum-based hydrocarbons, such as mineral oil and petrolatum, and water can also be used in suspension formulations.

[0054] Injectable dosage forms generally include aqueous or oily suspensions that can be prepared using appropriate dispersing or wetting agents and suspending agents. Injectable forms can be in solution phase or in the form of suspensions prepared with solvents or diluents. Acceptable solvents or vehicles include sterile water, Ringer's solution, or isotonic saline. Alternatively, sterile oils can be used as solvents or suspending agents. Preferably, the oil or fatty acid is non-volatile, including natural or synthetic oils, fatty acids, monoglycerides, diglycerides, or triglycerides.

[0055] For injection, the pharmaceutical formulation may be a powder suitable for reconstitution with an appropriate solution as described above. Examples include, but are not limited to, freeze-dried, rotary-dried or spray-dried powders, amorphous powders, granules, precipitates, or particles. For injection, the formulation may optionally include stabilizers, pH adjusters, surfactants, bioavailability modifiers, and combinations thereof. The compound may be formulated for parenteral administration by injection, such as bolus injection or continuous infusion. The unit dosage form for injection may be in ampoules or multi-dose containers. In addition to the above representative dosage forms, pharma- ceutical acceptable excipients and carriers are generally known to those skilled in the art and may be used. Such excipients and carriers are described, for example, in “Remingtons Pharmaceutical Sciences” Mack Pub. Co., New Jersey (1991), which is incorporated herein by reference.

[0056] The treprostinil prodrug can be formulated in a formulation suitable for parenteral administration, which may include a sterile aqueous formulation of the treprostinil prodrug or a pharma- ceutically acceptable salt thereof, which formulation may be isotonic with the blood of the intended recipient. These formulations may be administered by subcutaneous injection, although administration may also be by intravenous or intramuscular or intradermal injection. Such preparations may be conveniently prepared by mixing the compound with water or a glycine or citrate buffer and rendering the resulting solution sterile and isotonic with blood. The injectable formulation may contain 0.1-5% w / v based on the weight of treprostinil in the prodrug and may be administered at a rate of 0.1 ml / min / kg. Alternatively, the prodrug may be administered at a rate of 0.625-50 ng / kg / min based on the weight of treprostinil in the prodrug. Alternatively, the prodrug may be administered at a rate of 10-15 ng / kg / min based on the weight of treprostinil in the prodrug.

[0057] In some embodiments, the concentration of the treprostinil prodrug in a formulation for parenteral administration, such as intravenous or subcutaneous infusion (including continuous subcutaneous infusion), can be 0.0005-30 mg / mL, or 0.0007-50 mg / mL, or 0.001-15 mg / mL, or any value or subrange within these ranges. Exemplary concentrations can include 0.1 mg / mL, 1 mg / mL, 2.5 mg / mL, 5 mg / mL, or 10 mg / mL.

[0058] In some embodiments, formulations of treprostinil prodrugs for parenteral administration, such as intravenous or subcutaneous injection (including continuous subcutaneous infusion), can be prepared by mixing the prodrug with a vehicle, such as a buffer. In certain embodiments, the vehicle can be a phosphate-containing vehicle, i.e., at least one phosphate, which can be, for example, a dibasic phosphate, such as dibasic sodium phosphate or dibasic potassium phosphate, or a tribasic phosphate, such as tribasic sodium phosphate or tribasic potassium phosphate. In certain embodiments, the vehicle can also contain a halogen salt, such as a chloride salt, which can be sodium chloride or potassium chloride. A halogen salt, such as sodium chloride, can be used to adjust the tonicity of the vehicle. In certain embodiments, it may be preferred that the phosphate and the halogen salt have the same cation. For example, when the phosphate is sodium phosphate, such as tribasic sodium phosphate or tribasic sodium phosphate, the halogen salt can be a halogen salt, such as sodium chloride. Similarly, when the phosphate is potassium phosphate, such as tribasic potassium phosphate or tribasic potassium phosphate, the halogen salt can be a potassium halogen salt, such as potassium chloride. The solvent of the vehicle can include water. In certain embodiments, water can be the only solvent in the vehicle. In more specific embodiments, the vehicle can include one or more additional solvents in addition to water. In some embodiments, the additional solvent can be a preservative, such as m-cresol.

[0059] Preferably, the vehicle is isotonic with the blood of a patient, such as a human. The term isotonic may mean that the osmolality and ionic concentration of the vehicle are consistent with those of a patient, such as a human. Non-limiting examples of vehicles include phosphate buffered saline, which is an aqueous salt solution that includes disodium hydrogen phosphate, sodium chloride, and in some formulations, potassium chloride and potassium dihydrogen phosphate. Other examples may include a vehicle containing 20 mM dibasic sodium phosphate with 125 mM sodium chloride, and a vehicle containing 15 mM tribasic sodium phosphate, 125 mM sodium chloride, and 0.3% w / w m-cresol.

[0060] Treatment method Treprostinil prodrugs can be used to treat diseases or conditions treatable with treprostinil, i.e., diseases or conditions for which treprostinil is known to be effective. In some embodiments, such conditions can be pulmonary hypertension. In some embodiments, treprostinil prodrugs can be used to treat pulmonary arterial hypertension (PAH). In some embodiments, treprostinil prodrugs can be used to treat one or more of pulmonary hypertension in WHO groups 1-5. Similarly, treprostinil prodrugs described herein can be used to treat any disease or condition for which treprostinil is indicated or useful. Treprostinil prodrugs can be administered as the sole therapeutic agent or in addition to other active agents, including treprostinil.

[0061] In some embodiments, a method for treating a disease or condition is provided, comprising administering to a subject a compound (e.g., a prodrug) or composition disclosed herein.In some embodiments, the disease or condition is one or more selected from pulmonary hypertension, congestive heart failure, peripheral vascular disease, Raynaud's phenomenon, scleroderma, renal failure, peripheral neuropathy, digital ulcers, intermittent claudication, ischemic limb disease, peripheral ischemic lesions, pulmonary fibrosis, and asthma.In some embodiments, the disease is pulmonary hypertension.

[0062] Administration can be via the routes described above, or for example, orally, intravenously, intraarterially, intramuscularly, intranasally, rectally, intravaginally, or subcutaneously. In some embodiments, the composition is administered by injection. In some embodiments, administration is oral. In some embodiments, administration is subcutaneous.

[0063] The subject to be treated may be a human, dog, cat, bird, non-human primate, cow, or horse. In a preferred embodiment, the subject is a human.

[0064] In some embodiments, a method of treating a disease or condition is provided, the method comprising administering to a subject a prodrug of treprostinil, whereby said prodrug is converted to a metabolite that can comprise, consist essentially of, or consist of treprostinil.

[0065] The treprostinil prodrug can be administered in an effective amount to a subject, such as a human.

[0066] The term "effective amount" may refer to the amount of treprostinil prodrug that may be required to treat a disease or condition, such as pulmonary hypertension. In some embodiments, the effective amount of treprostinil prodrug may be the same or similar to the effective amount of treprostinil for treating the same disease or condition. In some embodiments, the effective amount of treprostinil prodrug may be different from the effective amount of treprostinil for treating the same disease or condition. One skilled in the art will be able to determine the "effective amount" of a treprostinil prodrug based on, for example, the relevant disease or condition, the amount of treprostinil known to treat, ameliorate, or prevent the disease or condition, and the rate at which the prodrug is converted to treprostinil in vivo.

[0067] Manufacturing method FDKP-Treprostinil prodrugs can be prepared from a doubly protected treprostinil moiety, in which the treprostinil moiety has two of its three hydroxyl groups protected with hydroxyl protecting groups.

[0068] [ka]

[0069] The doubly protected treprostinil moiety can be produced from unprotected treprostinil, i.e., the compound of formula (1), using, for example, the methods disclosed in Greene, Protective Groups in Organic Synthesis, which is incorporated herein by reference in its entirety.

[0070] To generate a cyclopentyl ring FDKP-treprostinil prodrug, the doubly protected treprostinil moiety can be a compound of formula (2a), and to generate a side chain FDKP-treprostinil prodrug, the doubly protected treprostinil moiety can be a compound of formula (2b).

[0071] [ka]

[0072] [ka]

[0073] In some embodiments, in the doubly protected treprostinil moiety, R 1 and R 2 (or R 3 ) is a protecting group. R 1 and R 2 (or R 3 ) may be the same or different. In some embodiments, R 1can be a silyl carboxylic acid protecting group or a benzyl carboxylic acid protecting group. Silyl carboxylic acid protecting groups can be, for example, trimethylsilyl, triethylsilyl, triisopropylsilyloxymethyl, triisopropylsilyl, t-butyldimethylsilyl, t-butyldiphenylsilyl, phenyldimethylsilyl. Benzyl carboxylic acid protecting groups are unsubstituted benzyl or substituted benzyl groups, i.e., -NO at one or more meta, ortho, or para positions. 2 , -CN, halogen (e.g., -F, -Cl, -Br, or -I), (C 1 ~C 3 ) alkyl, halo(C 1 ~C 3 ) alkyl, (C 1 ~C 3 ) alkoxy, and halo(C 1 ~C 3 ) alkoxy. In some embodiments, R 2 (or R 3 ) can be a silyl hydroxyl protecting group, for example, trimethylsilyl, triethylsilyl, tri-isopropylsilyloxymethyl, triisopropylsilyl, t-butyldimethylsilyl, t-butyldiphenylsilyl, phenyldimethylsilyl.

[0074] The doubly protected treprostinil moiety can be reacted with an FDKP moiety, such as a compound of formula (3), to generate a doubly protected FDKP-treprostinil compound, which can be a doubly protected FDKP-treprostinil compound of formula (4a) or (4b).

[0075] [ka]

[0076] [ka]

[0077] [ka] In the FDKP part, R 5 can be H.

[0078] The coupling reaction between the FDKP moiety and the double-protected treprostinil moiety can be carried out in the presence of a carbodiimide, which can be a water-soluble carbodiimide such as EDCI.The reaction between the FDKP moiety and the double-protected treprostinil moiety can be carried out in the presence of a nucleophilic catalyst, which can be a pyridine compound, such as DMAP.In some embodiments, the nucleophilic catalyst, such as DMAP, can be used together with an organic base, such as DIPEA or triethylamine.The nucleophilic catalyst, such as DMAP, can be present alone or together with an organic base in a polar solvent, such as DMA or DMF.The reaction between the FDKP moiety and the double-protected treprostinil moiety can be carried out in the presence of a coupling reagent selected from Table 1 below.

[0079] R 5 In a compound of formula (5a) or (5b) where R is a polymeric carrier, a fatty alcohol carrier, or a fatty amine carrier, the compound may be produced from a coupling reaction of a compound of formula (5a) or (5b), where R 5 is H, and a polymer, a fatty acid, or a fatty amine. In some embodiments, the coupling is dicyclohexylcarbodiimide (DCC) coupling, or a coupling performed using a reagent selected from Table 1 below. [Table 1]

[0080] The doubly protected FDKP-Treprostinil moiety can be deprotected to generate the unprotected FDKP-Treprostinil compound of formula (5a) or (5b).

[0081] [ka]

[0082] [ka]

[0083] In some embodiments, the deprotection of the doubly protected FDKP-Treprostinil compound of formula 4a (4b) is carried out by reacting R 1 and R 2 (R 3 This can be done in one step by cleaving the protecting groups in R 1 and R 2 (R 3 ) are the same type of protecting group, and deprotection of the doubly protected FDKP-Treprostinil compound of formula 4a (4b) can be carried out in one step. For example, 1 and R 2 (R 3 When both protecting groups in (4a) are silyl protecting groups, which may be the same or different, deprotection of the doubly protected FDKP-Treprostinil compound of formula 4a(4b) may be carried out in one step using a silyl ester or ether cleaving agent that is an acid or a fluoride, such as tetra-n-butylammonium fluoride.

[0084] In some further embodiments, the deprotection of the doubly protected FDKP-Treprostinil compound of formula 4a(4b) can be carried out in two steps: 1 The other is a step of cleaving the carboxyl protecting group with R 2 (R 3 In some embodiments, the cleavage is first performed by cleaving the hydroxyl protecting group with R 1 Then, R 2 (R 3 In some other embodiments, cleavage may be performed first on R 2 (R 3 ), then R 1 For example, R 1 is a benzyl carboxylic acid protecting group, and R 2 (or R 3When R is a silyl hydroxyl protecting group, e.g., trimethylsilyl, triethylsilyl, tri-isopropylsilyloxymethyl, triisopropylsilyl, t-butyldimethylsilyl, or t-butyldiphenylsilyl, phenyldimethylsilyl, a benzyl carboxylic acid protecting group can be replaced by R 1 to generate the mono-protected FDKP-Treprostinil compound of formula (6a) or (6b):

[0085] [ka] [ka]

[0086] R 1 Cleavage of the benzyl carboxylic acid protecting group in can be carried out using a chemoselective benzyl cleaving agent such as trimethyltin hydroxide, barium hydroxide, barium carbonate, cesium hydroxide, cesium carbonate, other alkali metal hydroxides and carbonates, tributyltin hydroxide, tetramethylammonium hydroxide, or tetrabutylammonium hydroxide.

[0087] The unprotected FDKP-Treprostinil compound of formula (5a) or (5b) can then be generated from the single protected FDKP-Treprostinil compound of formula (6a) or (6b). In some embodiments, the unprotected FDKP-Treprostinil compound of formula (5a) or (5b) can be generated from the single protected FDKP-Treprostinil compound of formula (6a) or (6b) by reaction with R 2 or R 3can be generated by cleaving the silyl ether hydroxyl protecting group in using a silyl ether cleaving agent, which can be an acid or a fluoride, such as tetra-n-butylammonium fluoride. In yet some other embodiments, the unprotected FDKP-Treprostinil compound of Formula (5a) or (5b) can be generated from the mono-protected FDKP-Treprostinil compound of Formula (6a) or (6b) by simply storing the mono-protected FDKP-Treprostinil compound of Formula (6a) or (6b) for an extended period of time, such as at least 10 hours, or at least 12 hours, or at least 14 hours, or at least 16 hours, or at least 18 hours, and reacting with R 2 or R 3 The silyl hydroxyl protecting group in may be generated by using a silyl ether cleaving agent, which may be an acid or a fluoride, such as tetra-n-butylammonium fluoride, to allow for cleavage of the silyl ether hydroxyl protecting group. For example, in some embodiments, storing the mono-protected FDKP-Treprostinil compound of formula (6a) or (6b) in low concentrations of trifluoroacetic acid (TFA), for example, up to about 0.005% to about 0.2%, or up to about 0.01% to about 0.1%, for an extended period of time, for example, at least 10 hours, or at least 12 hours, or at least 14 hours, or at least 16 hours, or at least 18 hours, can be used to generate a silyl hydroxyl protecting group in R 2 or R 3 This allows for cleavage of the silyl ether hydroxyl protecting group in

[0088] The FDKP-Treprostinil compound of formula (5a) or (5b) may be manufactured in batches with a purity of at least 90%, or at least 92%, or at least 93%, or at least 94%, or at least 95%, or at least 96%, or at least 97%, or at least 98%, or at least 98.5%. In some embodiments, the batches have a purity of at least 90%, or at least 92%, or at least 93%, or at least 94%, or at least 95%, or at least 96%, or at least 97%, or at least 98%, or at least 98.5%.

[0089] In some embodiments, the batch of the compound of formula (5a) may be substantially free of the compound of formula (5b). In some embodiments, the batch of the compound of formula (5b) may be substantially free of the compound of formula (5a). For example, in some embodiments, the batch of the compound of formula (5a) may contain less than 5%, or less than 4%, or less than 3%, or less than 2%, or less than 1%, or less than 0.5%, or less than 0.3%, or less than 0.1% of the compound of formula (5b). Similarly, in some embodiments, the batch of the compound of formula (5b) may contain less than 5%, or less than 4%, or less than 3%, or less than 2%, or less than 1%, or less than 0.5%, or less than 0.3%, or less than 0.1% of the compound of formula (5a).

[0090] The amount of the compound of Formula (5a) or (5b) in the batch can be, for example, at least 0.1 g, at least 0.3 g, at least 0.5 g, at least 0.8 g, at least 1 g, at least 1.2 g, at least 1.5 g, at least 2 g, at least 3 g, at least 4 g, or at least 5 g.

[0091] The embodiments described herein are further illustrated, but not limited, by the following examples. EXAMPLES

[0092] Treprostinil prodrugs having (E)-3,6-bis[4-(N-carbonyl-2-propenyl)amidobutyl]-2,5-diketopiperazine (FDKP) were synthesized. Specifically, FDKP-treprostinil cyclopentyl ring ester and FDKP-treprostinil side chain ester were synthesized as shown in Scheme 1 and Scheme 2, respectively.

[0093] Scheme 1: Synthesis of FDKP-treprostinil cyclopentyl ring ester (6) [ka]

[0094] Scheme 2: Synthesis of FDKP-Treprostinil side chain ester (9) [ka]

[0095] FDKP-Treprostinil Ester: The diprotected treprostinil moiety, side-chain TBDMS-treprostinil TMSE ester (1, R = TMSE), was coupled with (E)-3,6-bis[4-(N-carbonyl-2-propenyl)amidobutyl]-2,5-diketopiperazine (FDKP) (3) in the presence of EDCI.HCl and DMAP in DMA or DMF to give mono-TBDMS FDKP-treprostinil TMSE cyclopentyl ring ester (4, R = TMSE) in moderate yield after chromatographic separation on a reversed-phase pre-packed column using water and acetonitrile. Attempted desilylation of the TMSE and TBDMS groups from 4 using TBAF in THF gave a small amount of the desired product, FDKP-treprostinil cyclopentyl ring ester (6), along with the FDKP cleavage product, i.e., treprostinil. To overcome FDKP cleavage during desilylation, the protecting group TMSE was replaced with a benzyl group under acid protection. The benzyl-protected acid side-chain TBDMS treprostinil benzyl ester (2, R=benzyl) was used in the coupling reaction. Side-chain TBDMS treprostinil benzyl ester (2) was coupled with FDKP to produce TBDMS FDKP treprostinil benzyl cyclopentyl ring ester (5, R=benzyl) in moderate yield after reversed-phase column chromatography, which was analyzed by LCMS and NMR. 1The results were confirmed by H NMR. Chemoselective debenzylation of the crude (5, R=benzyl) with trimethyltin hydroxide in 1,2-dichloroethane gave the corresponding acid (5, R=H). There was a trace of FDKP cleavage product. Purification of the crude acid by reversed-phase column using 0.05% trifluoroacetic acid (TFA) in water and acetonitrile gave the desired FDKP-treprostinil cyclopentyl ring ester (6). Due to the weakly acidic nature of the 0.05% TFA solution, desilylation of TBDMS occurred while storing the combined fractions in a refrigerator overnight. This avoided an extra chemical step to cleave the TBDMS group to obtain the final compound 6. Lyophilization of the combined fractions gave the pure FDKP-treprostinil cyclopentyl ring ester (6) as an off-white solid, with IR, 1 The cyclopentyl TBDMS-treprostinil benzyl ester (7) was also coupled with FDKP (2) under similar reaction conditions to give the TBDMS FDKP-treprostinil benzyl side chain ester (3). Chemoselective debenzylation of the crude product (8) with trimethyltin hydroxide followed by desilylation during reversed-phase column chromatography afforded the FDKP-treprostinil side chain ester (9) in moderate yield, which was characterized by IR, NMR, and LCMS. 1 It was characterized by 1 H NMR and LCMS.

[0096] Synthesis method:

[0097] Coupling of diprotected treprostinil (1 or 2) with (E)-3,6-bis[4-(N-carbonyl-2-propenyl)amidobutyl]-2,5-diketopiperazine (FDKP) (3) was attempted using a variety of coupling reagents.

[0098] EDCI.HCl, DMAP, DIEA in N,N-Dimethylacetamide (DMA)

[0099] EDCI.HCl in DMA or N,N-dimethylformamide (DMF), DMAP

[0100] O-(benzotriazol-1-yl)-N,N,N',N'-tetramethylurolium tetrafluoroborate (TBTU) and DBU in DMA

[0101] 2-Chloro-1-methylpyridinium iodide (CMPI) and triethylamine in DMA

[0102] EDCI.HCL, DMAP, HOBt, DIPEA in DMA

[0103] Solid supported EDCI, solid supported DMAP in DMF.

[0104] Coupling reactions of diprotected treprostinil (1 or 2) with FDKP (3) using EDCI·HCl, DMAP in DMF or DMA gave the desired coupling products (4 or 5), while other reagents did not work (Schemes 1 and 2). Compound (4) was purified by reverse-phase column chromatography using acetonitrile and water as the mobile phase. Attempting cleavage of TBDMS and TMSE from compound (4) using tetrabutylammonium fluoride (TBAF) afforded small amounts of the desired compound (6) along with treprostinil (by cleavage of the ester bond), and similar results were observed when compound (5) was used. Chemoselective cleavage of the benzyl ester from compound (3b) was then performed by replacing TBAF with trimethyltin hydroxide to give the corresponding acid. Hydrolysis of the ester bond between the FDKP and treprostinil moieties was minimal when trimethyltin hydroxide was used. The crude acid after benzyl ester hydrolysis was purified by reverse phase column chromatography using 0.05% ACN in water / 0.05% TFA in ACN to give TBDMS FDKP-Treprostinil-cyclopentyl ring ester, the precursor of compound (6). The desired product was detected by LCMS. Upon storage of the purified product, cleavage of the TBDMS group occurred to produce the final product (6). Lyophilization of the combined pure fractions gave FDKP-Treprostinil-cyclopentyl ring ester (6) as an off-white solid. Pure compound (6) was identified by IR, 1 It was characterized by 1 H NMR, DQF-COSY, and LCMS.

[0105] Following the successful synthesis of FDKP-Treprostinil-cyclopentyl ring ester (6), similar reaction conditions were used to synthesize FDKP-Treprostinil-side chain ester (9) using biprotected treprostinil (7) and FDKP (3), as shown in Scheme 2. Pure FDKP-Treprostinil-side chain ester (9) was identified by IR, 1 It was characterized by 1 H NMR, DQF-COSY, and LCMS.

[0106] experiment:

[0107] Synthesis of TBDMS FDKP-Treprostinil TMSE cyclopentyl ring ester (4, R=TMSE):

[0108] To a solution of TBDMS-Treprostinil TMSE ester (1, R = TMSE) (0.50 g) in N,N-dimethylacetamide (DMA) (14 mL) was added (E)-3,6-bis[4-(N-carbonyl-2-propenyl)amidobutyl]-2,5-diketopiperazine (FDKP) (3) (0.44 g), EDCI.HCl (0.24 g), and DMAP (0.30 g) at room temperature under argon. The suspension reaction mixture was stirred and heated to 100 °C. The reaction mixture became clear and light brown and was continued to heat at 100 °C overnight. The reaction mixture was removed from heating and then evaporated in vacuo to remove DMA and other organic volatiles. The crude product was triturated with toluene (75 mL) and the toluene soluble product was decanted (repeated two more times). The toluene-insoluble product was dried under vacuum to give a light brown solid (0.61 g). The crude product was chromatographed on an inverted pre-packed column using a mixture of water and acetonitrile to give TBDMS-treprostinil TMSE cyclopentyl FDKP ester (4, R=TMSE) (0.015 g), confirmed by LCMS.

[0109] Synthesis of FDKP-Treprostinil Cyclopentyl Ring Ester (6):

[0110] To a solution of TBDMS FDKP-Treprostinil TMSE cyclopentyl ring ester (4, R=TMSE) (0.013 g) in dimethyl sulfoxide (0.75 mL) was added a solution of tetrabutylammonium fluoride (TBAF) (1.0 M in THF (0.12 mL)) at room temperature under argon. The reaction mixture was stirred at room temperature overnight. After 22 hours, the reaction mixture was checked by LCMS, which showed the desired molecular weight of FDKP-Treprostinil cyclopentyl ring ester (6) along with the FDKP cleavage product, i.e., Treprostinil.

[0111] Synthesis of TBDMS FDKP-Treprostinil benzyl cyclopentyl ring ester (5, R=benzyl):

[0112] To a solution of mono-TBDMS-Treprostinil benzyl ester (6, R=benzyl) (3.17 g) in N,N-dimethylformamide (DMF) (90 mL) was added (E)-3,6-bis[4-(N-carbonyl-2-propenyl)amidobutyl]-2,5-diketopiperazine (FDKP) (3) (2.89 g), EDCI.HCl (1.54 g), and DMAP (1.54 g) at room temperature under argon. The suspension reaction mixture was stirred and heated to 100° C. The reaction mixture turned brown and cloudy and was continued to heat at 100° C. overnight. After 16 h, the reaction mixture was checked by LCMS for the desired product. Two more batches of coupling reaction of 0.61 g of TBDMS-Treprostinil benzyl ester (2, R = benzyl) with 0.55 g of (E)-3,6-bis[4-(N-carbonyl-2-propenyl)amidobutyl]-2,5-diketopiperazine (FDKP) (3) were carried out under similar reaction conditions and checked by LCMS. These three batches were combined based on LCMS data. The reaction mixture was evaporated in vacuum to remove DMF and other organic volatiles to give a dark brown viscous liquid (18.37 g). The crude product was triturated with dichloromethane (3 x 100 mL) and filtered. The dichloromethane insoluble product (brown solid) was discarded and the dichloromethane soluble product was concentrated under vacuum to give a brown viscous semi-solid product (14.59 g). The crude product was triturated with toluene (3 x 100 mL) and filtered. The toluene soluble product (7.02 g after toluene evaporation) was discarded. The toluene insoluble product was dried under vacuum to give a brown semi-solid (5.02 g). The crude product (5.0 g) was chromatographed on a silica gel (188 g) column (230-400 mesh) using methanol (0-100%) in dichloromethane to give TBDMS FDKP-treprostinil benzyl cyclopentyl ring ester (5, R = benzyl) (2.26 g), confirmed by LCMS. The purified product contained a small amount of DMAP, which could be removed in the next step.1.0 g of this purified product was further purified by reversed-phase pre-packed column chromatography using a mixture of water and acetonitrile containing 0.05% trifluoroacetic acid to give pure TBDMS FDKP-treprostinil benzyl cyclopentyl ring ester (5, R=benzyl) (0.085 g), which was confirmed by LCMS.

[0113] Synthesis of FDKP treprostinil cyclopentyl ring ester (14):

[0114] To a solution of crude mono-TBDMS FDKP treprostinil benzyl cyclopentyl ring ester (13, R=benzyl) (1.04 g) in 1,2-dichloroethane (25 mL) was added trimethyltin hydroxide (1.46 g) at room temperature under argon. The reaction mixture was heated at 60° C. overnight. After 19 hours, the reaction was checked by LCMS and found to be complete. The reaction mixture was evaporated in vacuum to give a light orange solid (2.91 g). The crude product (1.45 g) was further purified by chromatography on a reversed-phase pre-packed column using a mixture of water and acetonitrile containing 0.05% trifluoroacetic acid to give pure fractions. After checking the LCMS of selected fractions, these fractions were combined. The combined fractions were stored in a refrigerator overnight. After 18 hours, the TBDMS group was completely cleaved, as confirmed by LCMS. The remaining crude product (1.45 g) was purified similarly. Both purified products were then combined and lyophilized to give the off-white FDKP-treprostinil cyclopentyl ring ester (6) as a white solid (0.212 g) with IR, 1 Characterized by 1 H NMR and LCMS.

[0115] Synthesis of mono-TBDMS FDKP-treprostinil benzyl side chain ester (8):

[0116] To a solution of TBDMS-Treprostinil benzyl ester (7) (5.57 g) in N,N-dimethylformamide (DMF) (150 mL) was added (E)-3,6-bis[4-(N-carbonyl)-2-propenyl)amidobutyl]-2,5-diketopiperazine (FDKP) (3) (5.08 g), EDCI.HCl (2.69 g), and DMAP (3.43 g) at room temperature under argon. The suspension reaction mixture was stirred and heated to 100° C. The reaction mixture turned brown and cloudy and was continued to heat at 100° C. overnight. After 16 hours, the reaction mixture was checked by LCMS for the desired product. The reaction mixture was evaporated in vacuo to remove DMF and other organic volatiles to give a brown viscous liquid (22.86 g). The crude product was triturated with dichloromethane (3×100 mL) and filtered. The dichloromethane insoluble product (brown solid) was discarded and the dichloromethane soluble product was concentrated under vacuum to give a brown viscous liquid product (17.33 g). The crude product was triturated with toluene (200 mL) and filtered. The toluene soluble product (semi-solid product after toluene evaporation 9.30 g) was discarded. The toluene insoluble product was dried under vacuum to give a brown foamy solid (7.73 g). LCMS of this crude product showed the desired TBDMS FDKP treprostinil benzyl side chain ester (8) (7.73 g) as the major product. The crude product was used in the next step without further purification.

[0117] Synthesis of FDKP-Treprostinil side chain ester (9):

[0118] To a solution of crude mono-TBDMS FDKP treprostinil benzyl side chain ester (8) (2.45 g) in 1,2-dichloroethane (100 mL) was added trimethyltin hydroxide (3.44 g) at room temperature under argon. The reaction mixture was heated at 60° C. overnight. After 17 hours, the reaction mixture was checked by LCMS and found to be complete. The reaction mixture was cooled to room temperature. The dichloroethane layer was decanted from the brown viscous liquid (gummy). The dichloroethane layer was evaporated under vacuum to give a light brown viscous liquid (4.48 g). LCMS of this product showed very little product. The gummy brown product was dried under high vacuum to give a foamy brown solid (1.02 g) which contained a large amount of the desired product. The crude product (1.0 g) was purified by chromatography on a reversed-phase pre-packed column using a mixture of water and acetonitrile containing 0.05% trifluoroacetic acid to give pure fractions. After checking the LCMS of selected fractions, these fractions were combined. The combined fractions were stored in the refrigerator overnight. After 16 hours, the TBDMS group was completely cleaved, as confirmed by LCMS. The purified products were combined and lyophilized to give the off-white FDKP-Treprostinil side chain ester (9) as an off-white solid (0.195 g) with IR, 1 It was characterized by 1 H NMR and LCMS and had a purity of 98.85% by HPLC.

[0119] Although the above refers to certain preferred embodiments, it will be understood that the present invention is not limited thereto. Those skilled in the art will recognize that various modifications can be made to the disclosed embodiments, and such modifications are intended to be within the scope of the present invention.

[0120] All publications, patent applications, and patents cited herein are hereby incorporated by reference in their entirety.

Claims

1. A compound of formula 5a or 5b: 【Chemistry 1】 【Chemistry 2】 or a pharma- ceutically acceptable salt thereof (In the formula R ５ is H).

2. The compound of claim 1 , wherein the compound is a compound of formula 5a.

3. 2. The compound of claim 1, wherein the compound is a compound of formula 5b.

4. R ５ The compound of claim 1 , wherein

5. 10. A pharmaceutical composition comprising a compound of claim 1 and a pharma- ceutically acceptable excipient.

6. 1. A method for producing an FDKP-Treprostinil compound, comprising: i) Treprostinil (1) 【Chemistry 3】 to form a doubly protected treprostinil moiety (2). 【Chemistry 4】 wherein R １ is a carboxylic acid protecting group, and a) R ２ is H, and R ３ is a hydroxyl protecting group, or b) R ２ is a hydroxyl protecting group, R ３ is H); ii) synthesizing the doubly protected treprostinil moiety into a compound of formula (3): 【Chemistry 5】 (In the formula, R ４ is H, and R ５ is H) to produce a doubly protected FDKP-Treprostinil compound having the following formula (4a) or (4b): 【Chemistry 6】 【Chemistry 7】 and iii) deprotecting the doubly protected FDKP-Treprostinil compound to obtain an FDKP-Treprostinil compound having the following formula (5a) or (5b): 【Chemistry 8】 【Chemistry 9】 generating A method comprising:

7. R １ The method of claim 6 , wherein is a silyl carboxylic acid protecting group or a substituted or unsubstituted benzyl carboxylic acid protecting group.

8. R １ 8. The method of claim 7, wherein is a silyl carboxylic acid protecting group selected from trimethylsilyl, triethylsilyl, tri-isopropylsilyloxymethyl, triisopropylsilyl, t-butyldimethylsilyl, t-butyldiphenylsilyl, and phenyldimethylsilyl.

9. R １ The method of claim 7, wherein is a substituted or unsubstituted benzyl carboxylic acid protecting group.

10. R ２ is H, and R ３ The method of claim 6, wherein: is a hydroxyl protecting group, the doubly protected FDKP-treprostinil compound has the formula (4b), and the FDKP-treprostinil compound has the formula (5b).

11. R ３ is H, and R ２ The method of claim 6, wherein: is a hydroxyl protecting group, the doubly protected FDKP-treprostinil compound has the formula (4a), and the FDKP-treprostinil compound has the formula (5a).

12. 7. The method of claim 6, wherein reacting the doubly protected treprostinil moiety with the compound of formula (3) is carried out in the presence of a carbodiimide and a nucleophilic catalyst in a polar solvent.

13. The method of claim 12, wherein the reaction is carried out in the presence of an organic base.

14. The deprotection step deprotects the doubly protected FDKP-Treprostinil compound to produce a mono-protected FDKP-Treprostinil compound of formula (6a) or (6b): 【Chemistry 10】 【Chemistry 11】 10. The method of claim 9, comprising generating

15. 15. The method of claim 14, wherein deprotecting the doubly protected FDKP-treprostinil compound to produce the mono-protected FDKP-treprostinil compound is carried out in the presence of a chemoselective benzyl cleavage agent.

16. 7. The method of claim 6, wherein the FDKP-Treprostinil compound is at least 90% pure.

17. 13. A pharmaceutical composition for treating a condition treatable with treprostinil, comprising a compound according to claim 1.

18. 18. The pharmaceutical composition of claim 17, wherein the condition is pulmonary hypertension.

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