Plant growth promotor, producing method thereof, concentrated liquid agent, plant growth promotion kit, and plant growth promotion method

Abstract

To provide agents, kits and methods that exhibit more excellent plant growth promotion effect compared with single use of humic acid.SOLUTION: A plant growth promotor includes, as an active ingredient, humic acid and one or more types of amino acids, with a melanic index of the humic acid being 2 or more, and the one or more types of amino acids being derived from enzymatic decomposition product or hydrolysates of animal and plant origin.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a plant growth promoter, a method for producing the same, a concentrated liquid preparation, a kit for promoting plant growth, and a method for promoting plant growth. [Background technology]

[0002] Humic substances are a general term for organic matter (non-living organic matter) whose chemical structure cannot be specified and which is produced when the remains of living organisms are broken down by the action of microorganisms, etc., and can generally be classified based on their solubility in alkali and acid into humic acids, which dissolve in alkali and / or acid, and humins, which do not dissolve in alkali or acid. For example, Patent Document 1 describes the use of fulvic acid, a type of humic acid, as a plant vitalizing agent. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] JP 2003-171215 A Summary of the Invention [Problem to be solved by the invention]

[0004] However, the present inventors have found a problem in that when humic acid is applied alone, the growth of the plant tends to soon catch up with that of a control plant to which humic acid is not applied.

[0005] In view of such problems, the present invention aims to provide an agent that exhibits a superior plant growth-promoting effect compared to the application of humic acid alone, as well as a production method, concentrated liquid agent, kit, and method thereof. [Means for solving the problem]

[0006] The present inventors conducted intensive research to solve the above problems and discovered that a combination of humic acid and one or more amino acids can provide a higher plant growth-promoting effect than humic acid alone, thereby completing the present invention.

[0007] That is, the present invention relates to the following [1] to

[33] . [1] A plant growth promoter comprising, as active ingredients, humic acid and one or more amino acids, The melanic index of humic acid is 2 or more, A plant growth promoter, wherein one or more types of amino acids are derived from enzymatic decomposition or hydrolysis products of raw materials of animal or plant origin. [2] The plant growth promoter described in [1], wherein one or more types of amino acids are derived from an enzymatic hydrolysis product of soybean-derived raw materials or a hydrolysate of milk protein. [3] The plant growth promoter described in [1] or [2], wherein the one or more types of amino acids are one or more types selected from 20 types of α-amino acids. [4] The plant growth promoter according to any one of [1] to [3], wherein a total mass of the one or more amino acids relative to the total mass of humic acid is 1% to 55%. [5] The plant growth promoter according to any one of [1] to [4], wherein the enzyme comprises papain. [6] The plant growth promoter according to any one of [1] to [5], wherein the humic acid comprises one or more selected from the group consisting of humic acid, fulvic acid, nitrohumic acid, potassium humate, ammonium humate, calcium humate, magnesium humate, ammonium nitrohumate, calcium nitrohumate, magnesium nitrohumate, potassium fulvic acid, ammonium fulvic acid, calcium fulvic acid, magnesium fulvic acid, ammonium nitrofulvic acid, calcium nitrofulvic acid, and magnesium nitrofulvic acid. [7] A method for producing the plant growth-promoting agent according to any one of [1] to [6], comprising mixing humic acid and one or more amino acids. [8] A plant growth promoter characterized by being used in combination with one or more types of amino acids, the plant growth promoter containing humic acid having a melanic index of 2 or more as an active ingredient, and the one or more types of amino acids being hydrolysates or enzymatic decomposition products of raw materials derived from animals or plants. [9] The plant growth promoter described in [8], wherein one or more types of amino acids are derived from an enzymatic hydrolysis product of soybean-derived raw materials or a hydrolysate of milk protein.

[10] The plant growth promoter described in [8] or [9], wherein the one or more types of amino acids include at least 16 types of α-amino acids.

[11] The plant growth promoter according to any one of [8] to

[10] , wherein a total mass of the one or more amino acids relative to the total mass of humic acid is 1% to 55%.

[12] The plant growth promoter according to any one of [8] to

[11] , wherein the enzyme comprises papain.

[13] Humic acid may also be called humic acid, fulvic acid, nitrohumic acid, potassium humate, ammonium humate, calcium humate, magnesium humate, ammonium nitrohumate, calcium nitrohumate, magnesium nitrohumate, potassium fulvic acid, ammonium fulvic acid, calcium fulvic acid, magnesium fulvic acid, or nitrofulvic acid. The plant growth promoter according to any one of [8] to

[12] , comprising one or more selected from the group consisting of ammonium, calcium nitrofulvate, and magnesium nitrofulvate.

[14] A concentrated liquid preparation that, upon dilution with water, constitutes the plant growth promoter according to any one of [1] to [6] or the plant growth promoter according to any one of [8] to

[13] .

[15] A kit for promoting plant growth comprising humic acid and one or more amino acids, wherein the humic acid has a melanic index of 2 or more, and the one or more amino acids are hydrolysates or enzymatic decomposition products of raw materials derived from animals or plants.

[16] The kit described in

[15] , wherein one or more types of amino acids are derived from an enzymatic hydrolysis product of soybean-derived material or a hydrolysate of milk protein.

[17] The kit described in

[15] or

[16] , wherein the one or more types of amino acids include at least 16 types of α-amino acids.

[18] The kit described in any of

[15] to

[17] , wherein the total mass of the one or more amino acids relative to the total mass of humic acid is 1% to 55%.

[19] The kit according to any one of

[15] to

[18] , wherein the enzyme comprises papain.

[20] The kit according to any one of

[15] to

[19] , wherein the humic acid comprises one or more selected from the group consisting of humic acid, fulvic acid, nitrohumic acid, potassium humate, ammonium humate, calcium humate, magnesium humate, ammonium nitrohumate, calcium nitrohumate, magnesium nitrohumate, potassium fulvic acid, ammonium fulvic acid, calcium fulvic acid, magnesium fulvic acid, ammonium nitrofulvic acid, calcium nitrofulvic acid, and magnesium nitrofulvic acid.

[21] A method for promoting plant growth, comprising applying humic acid and one or more amino acids, wherein the humic acid has a melanic index of 2 or more, and the one or more amino acids are derived from an enzymatic decomposition or hydrolysis product of raw materials of animal or plant origin.

[22] The method according to

[21] , wherein one or more types of amino acids are derived from an enzymatic hydrolysis product of a soybean-derived material or a milk protein hydrolysate.

[23] The method according to

[22] , wherein the one or more types of amino acids include at least 16 types of α-amino acids.

[24] The method according to any of

[21] to

[23] , wherein the total mass of the one or more amino acids relative to the total mass of humic acid is 1% to 55%.

[25] The method according to any one of

[21] to

[24] , wherein the enzyme comprises papain.

[26] The method according to any of

[21] to

[25] , wherein the humic acid comprises one or more selected from the group consisting of humic acid, fulvic acid, nitrohumic acid, potassium humate, ammonium humate, calcium humate, magnesium humate, ammonium nitrohumate, calcium nitrohumate, magnesium nitrohumate, potassium fulvic acid, ammonium fulvic acid, calcium fulvic acid, magnesium fulvic acid, ammonium nitrofulvic acid, calcium nitrofulvic acid, and magnesium nitrofulvic acid.

[27] Use of a composition comprising humic acid and one or more amino acids for promoting plant growth, wherein the humic acid has a melanic index of 2 or more, and the one or more amino acids are derived from an enzymatic decomposition or hydrolysis product of an animal or plant-derived material.

[28] Use of a composition containing humic acid having a melanic index of 2 or more for promoting plant growth, 1. Use of the present invention, characterized in that it is used in combination with one or more kinds of amino acids, the one or more kinds of amino acids being hydrolysates or enzymatic decomposition products of raw materials derived from animals or plants.

[29] The use described in

[27] or

[28] , wherein one or more types of amino acids are derived from an enzymatic hydrolysis product of soybean-derived material or a hydrolysate of milk protein.

[30] The use described in any of

[27] to

[29] , wherein the one or more types of amino acids include at least 16 types of α-amino acids.

[31] The use according to any one of

[27] to

[30] , wherein the total mass of the one or more amino acids relative to the total mass of humic acid is 1% to 55%.

[32] The use described in any one of

[27] to

[31] , wherein the enzyme includes papain.

[33] The use according to any one of

[27] to

[32] , wherein the humic acid comprises one or more selected from the group consisting of humic acid, fulvic acid, nitrohumic acid, potassium humate, ammonium humate, calcium humate, magnesium humate, ammonium nitrohumate, calcium nitrohumate, magnesium nitrohumate, potassium fulvic acid, ammonium fulvic acid, calcium fulvic acid, magnesium fulvic acid, ammonium nitrofulvic acid, calcium nitrofulvic acid, and magnesium nitrofulvic acid. Effect of the Invention

[0008] According to the present invention, a higher plant growth promoting effect can be obtained than when humic acid is used alone. [Brief description of the drawings]

[0009] [Figure 1]1 is a graph showing the leaf area on days 0, 4, 7, and 12 after application of humic acid and / or casamino acid in Example 1. [Diagram 2] 1 is a graph showing the leaf area on days 0, 4, 7, and 12 after application of humic acid and / or soy peptone in Example 1. [Diagram 3] 1 is a graph showing the leaf area 12 days after application of humic acid and / or casamino acid in Example 1 (Dunnett's test*: p<0.05). [Figure 4] 1 is a graph showing the leaf area 12 days after application of humic acid and / or soy peptone in Example 1 (Dunnett's test*: p<0.05). [Diagram 5] 1 shows an outline of the cultivation test division in Example 3. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] A plant growth promoter according to one embodiment of the present invention contains, as active ingredients, humic acid and one or more amino acids, wherein the humic acid has a melanic index of 2 or more, and the one or more amino acids are derived from an enzymatic decomposition product or hydrolysis product of an animal or plant-derived raw material.

[0011] Humic acid includes at least one of the group consisting of humic acid and humate salts. Examples of humic acid include natural humic acid that is naturally produced in peat and weathered coal, artificial humic acid that is artificially produced, and humate salts obtained by neutralizing natural humic acid or artificial humic acid with alkaline substances such as sodium, potassium, ammonia, calcium, and magnesium. Examples of artificial humic acid include artificial humic acid derived from lignite and artificial humic acid derived from brine. Artificial humic acid derived from lignite can be produced, for example, by nitric acid oxidation of lignite. Artificial humic acid derived from brine can be produced, for example, by the method described in JP 2003-171215 A.

[0012] The humic acid may be a humic acid extract. The humic acid extract refers to an extract obtained by extracting nitric acid oxides of young coal such as lignite and brown coal in the range of pH 2 to 8, preferably in the range of pH 2 to 7. The humic acid extract is obtained, for example, by stirring a mixture of nitric acid oxides of young coal (hereinafter referred to as humic acid crude product) obtained by oxidatively decomposing young coal with nitric acid, an inorganic compound containing at least one monovalent or divalent alkali selected from potassium hydroxide, sodium hydroxide, ammonium hydroxide, magnesium hydroxide, and calcium hydroxide, and water at 40 to 90°C for 0.5 to 1 hour, and then performing a solid-liquid separation process, thereby obtaining a liquid material. The inorganic compound is added to the water so that the pH is in the range of 5 to 8. A method for producing a humic acid extract is described in Japanese Patent No. 6231059. From the viewpoint of the plant growth promoting effect, the humic acid is preferably a humic acid extract derived from lignite.

[0013] Examples of humic acid include humic acid, fulvic acid, nitrohumic acid, potassium humate, ammonium humate, calcium humate, magnesium humate, ammonium nitrohumate, calcium nitrohumate, magnesium nitrohumate, potassium fulvic acid, ammonium fulvic acid, calcium fulvic acid, magnesium fulvic acid, ammonium nitrofulvic acid, calcium nitrofulvic acid, and magnesium nitrofulvic acid. From the viewpoint of the plant growth promotion effect, it is preferable that the humic acid contains one or more selected from the group consisting of the above humic acid and humic acid salts. The humic acid may contain two or more, three or more, four or more, or five or more selected from the group consisting of the above humic acid and humic acid salts, may contain humic acid and / or humic acid salts, may contain fulvic acid and / or fulvic acid salts, or may contain a combination of humic acid and / or humic acid salts and fulvic acid and / or fulvic acid salts.

[0014] The melanic index (MI) of humic acid is 2 or more, preferably 2.0 or more. MI is an index used to classify humic acid, and is the ratio of absorbance at wavelengths of 450 nm and 520 nm in the absorption spectrum of a sodium hydroxide extract (A450 / A520). (Kyōichi Kumada, Chemistry of Soil Organic Matter, 2nd Edition, Academic Press Center (1981); Sadahiro Yamamoto et al., "Simple Estimation of Humic Acid Type by Melanic Index," Japanese Journal of Soil and Plant Nutrition Science, Vol. 71, No. 1, pp. 82-85 (2000)).

[0015] More specifically, the MI is calculated by the following method. The sample is crushed into 250 μm sieve using a mortar and a 250 μm sieve. Approximately 10 g of the crushed sample is weighed in a weighing bottle with a known mass. The weighing bottle is left in a dryer maintained at 105°C for approximately 12 hours, and then returned to room temperature in a desiccator and weighed again. The weight loss is regarded as moisture and the moisture content of the sample is calculated. Next, 0.10 g of the 250 μm sieve in dry weight equivalent and 45 ml of 0.5 mol / L sodium hydroxide solution are placed in a 50 ml centrifuge tube, and the tube is shaken at 250 rpm for approximately 1 hour at room temperature of 20°C, and then centrifuged at 3,000×g for approximately 10 minutes, and the supernatant is filtered through Advantec No. 5C filter paper. The absorbance of the filtrate at 450 nm and 520 nm is measured using distilled water as a blank. In this case, if the absorbance at 450 nm is 1.0 or more, add 0.1 mol / L sodium hydroxide aqueous solution to adjust the absorbance to 0.8 or more but less than 1.0, and then measure the absorbance at 520 nm. Calculate the ratio (absorbance at 450 nm / absorbance at 520 nm) to obtain the MI.

[0016] The upper limit of MI is not particularly limited, but can be set to 6 or less, preferably 6.0 or less. The numerical range of MI is preferably 2.0 to 5.5.

[0017] The total organic carbon (TOC) concentration of the humic acid extract is preferably 5,000 mg / L or more. The TOC concentration of the humic acid extract is preferably 200,000 mg / L or less. The TOC concentration of the humic acid extract may be, for example, 20,000 to 150,000 mg / L, 80,000 to 120,000 mg / L, or 100,000 to 150,000 mg / L.

[0018] The method for measuring the TOC concentration of the extract is defined as follows. The extract of humic acid crude is centrifuged at 3,000 x g, and the supernatant is measured using a total organic carbon meter (Shimadzu TOC-L) with a combustion catalytic oxidation method. If it contains non-humic substances such as urea, a fertilizer component, the fractions (humic acid and fulvic acid fractions) are separated according to the International Humic Substances Society method (Fujitake, Humic Substances Research Vol. 3, p. 1-9) and quantified using the above method, and the TOC concentration of the extract is measured.

[0019] The proportion of humic acid in the plant growth promoter according to this embodiment is not particularly limited, but may be, for example, in the range of 20 ppm to 1000 ppm based on the total mass of the plant growth promoter, and may be 30 ppm or more, 40 ppm or more, 800 ppm or less, 500 ppm or less, or 200 ppm or less.

[0020] The target plant of the plant growth promoter according to the present embodiment may be a seed plant, a fern, or a moss, and the seed plant may be a gymnosperm or angiosperm, and the angiosperm may be a monocotyledonous plant or a dicotyledonous plant. The seed plant also includes plants that reproduce without forming seeds, for example, vegetatively propagated plants that reproduce by bulbs or tubers. From a commercial viewpoint, it is preferable to target one or more types of the group consisting of vegetables and ornamental plants.

[0021] Specific examples of such plants include plants from the Brassicaceae family, Poaceae family, Solanaceae family, Asteraceae family, Cucurbitaceae family, Apiaceae family, Rosaceae family, Liliaceae family, Orchidaceae family, Amaryllidaceae family, Primulaceae family, Fabaceae family, Onionaceae family, Polygonaceae family, Convolvulaceae family, Chenopodiaceae family, Vitaceae family, Rutaceae family, Ebenaceae family, Theaceae family, Oleaceae family, Malvaceae family, Musaceae family, Zingiberaceae family, Rubiaceae family, and Bromeliaceae. Examples of plants from the Brassicaceae family include komatsuna (Japanese mustard spinach), bok choy, turnip, cauliflower, cabbage, radish, Chinese cabbage, and broccoli. Examples of plants from the Poaceae family include rice, barley, wheat, sugarcane, and corn. Examples of plants from the Solanaceae family include tomato, tobacco, shishito pepper, potato, chili pepper, eggplant, paprika, and green pepper. Examples of plants in the Asteraceae family include lettuce, artichoke, burdock, chrysanthemum, chrysanthemum, and sunflower. Examples of plants in the Cucurbitaceae family include pumpkin, cucumber, watermelon, and melon. Examples of plants in the Umbelliferae family include parsley, celery, carrot, and parsley. Examples of plants in the Rosaceae family include strawberry, rose, apple, pear, peach, loquat, and almond. Examples of plants in the Liliaceae family include asparagus, tulip, and lily. Examples of plants in the Orchidaceae family include orchids and cymbidium. Examples of plants in the Amaryllidaceae family include daffodils. Examples of plants in the Primulaceae family include cyclamen. Examples of plants in the Fabaceae family include soybeans, kidney beans, and adzuki beans. Examples of plants in the Liliaceae family include green onions, Chinese chives, shallots, and garlic. Examples of plants in the Polygonaceae family include buckwheat. Examples of plants in the Convolvulaceae family include sweet potatoes. Examples of plants in the Chenopodiaceae family include spinach and sugar beet. Examples of plants in the Vitaceae family include grapes. Examples of plants in the Rutaceae family include mandarin oranges, lemons, and oranges. Examples of plants in the Ebenaceae family include persimmons. Examples of plants in the Theaceae family include tea. Examples of plants in the Oleaceae family include olives and jasmine. Examples of plants in the Malvaceae family include cotton, cacao, and okra. Examples of plants in the Musaceae family include bananas. Examples of plants in the Zingiberaceae family include ginger. Examples of plants in the Rubiaceae family include coffee trees. Examples of plants in the Bromeliaceae family include pineapples and ananas.

[0022] The one or more amino acids may be proteinogenic amino acids, free amino acids, or a mixture thereof.

[0023] In this embodiment, the one or more amino acids are derived from an enzymatic decomposition or hydrolysis product of an animal or plant-derived raw material. "Derived from an enzymatic decomposition or hydrolysis product" means that the one or more amino acids may be present in a form contained in the enzymatic decomposition or hydrolysis product, or may be isolated or purified from the enzymatic decomposition or hydrolysis product.

[0024] The raw material of animal or plant origin is not particularly limited as long as it contains protein, and may be an animal or plant-derived protein. Examples of the raw material of animal origin include raw materials of milk origin, meat of pork, beef, chicken, etc., fish meat of bonito, tuna, etc., insects, etc. Examples of the raw material of plant origin include raw materials of soybean origin, raw materials of pea origin, raw materials of wheat origin, raw materials of corn origin, raw materials of leafy vegetables and fruit vegetables, etc.

[0025] Enzymatic decomposition products of raw materials of animal and plant origin are obtained by decomposing raw materials of animal and plant origin with enzymes. Examples of enzymes that decompose proteins (proteases) include endopeptidases such as papain, pepsin, pancreatin, and trypsin, and exopeptidases such as aminopeptidase and carboxypeptidase. The enzymes may include one or more, two or more, three or more, four or more, or five types selected from the group consisting of papain, pepsin, trypsin, aminopeptidase, and carboxypeptidase. When enzymatic decomposition of raw materials of plant origin is performed, the enzymes preferably include papain.

[0026] The hydrolysates of raw materials of animal or plant origin can be obtained by hydrolyzing the raw materials of animal or plant origin with an acid or an enzyme such as a protease. Examples of the acid include hydrochloric acid, sulfuric acid, and nitric acid.

[0027] From the viewpoint of the plant growth-promoting effect, the one or more types of amino acids are preferably derived from an enzymatic hydrolysis product of a soybean-derived raw material or a milk protein hydrolysate.

[0028] When the animal / plant-derived raw material is a soybean-derived raw material, examples of the soybean-derived raw material include soybean flour, soybean isolate, defatted soybean meal, and soybean lees. Enzymatic hydrolysis products can be obtained by decomposing these raw materials with an enzyme such as papain. Such hydrolysis products contain 16 or more kinds of amino acids. The enzymatic hydrolysis product of the soybean-derived raw material may be soy peptone. Soy peptone may be prepared by digesting defatted soybean flour with papain, or a commercially available product (e.g., product number 18889-95 from Nacalai Tesque, Inc.) may be used.

[0029] When the animal / plant-derived raw material is a milk-derived raw material, examples of the milk-derived raw material include milk proteins (whey, casein, etc.). A hydrolysate can be obtained by hydrolyzing milk proteins with an acid such as hydrochloric acid or an enzyme such as protease. Such hydrolysates contain 16 or more kinds of amino acids. The milk protein hydrolysate may be casamino acids obtained by hydrolyzing casein. Casamino acids may be prepared by hydrolyzing casein, or commercially available products (for example, product number A1404HA from Fujifilm Wako Pure Chemical Industries, Ltd.) may be used.

[0030] Examples of amino acids include alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine.

[0031] The one or more kinds of amino acids may include, for example, at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, or 19 kinds selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine (lysine), methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine, or may include all of these. From the viewpoint of the plant growth-promoting effect, it is preferable that the one or more kinds of amino acids include at least 16 kinds of amino acids. The amino acids are preferably α-amino acids. An α-amino acid is an amino acid in which an amino group is also bonded to the carbon (α carbon) to which a carboxy group is bonded, and is represented by the general formula RCH(NH2)COOH.

[0032] The percentage (%) of the total mass of amino acids or free amino acids contained in the enzymatic decomposition or hydrolysis product of raw materials of animal or plant origin can be measured by high performance liquid chromatography (HPLC).

[0033] The percentage (%) of the total mass of amino acids contained in the enzymatic decomposition product or hydrolysate of the animal / plant-derived raw material may be, for example, 40% to 80%, 45% to 80%, 50% to 80%, 45% to 70%, or 50% to 65% based on the entire enzymatic decomposition product or hydrolysate.

[0034] The percentage (%) of the total mass of free amino acids contained in the enzymatic hydrolysate of the plant-derived raw material may be, for example, 3% to 30%, 5% to 25%, 10% to 25%, 15% to 25%, or 15% to 20% based on the entire enzymatic hydrolysate.

[0035] The percentage (%) of the total mass of free amino acids contained in the hydrolysate of animal-derived raw materials may be, for example, 40% to 80%, 43% to 80%, 45% to 80%, 45% to 70%, or 45% to 60% based on the entire hydrolysate.

[0036] The plant growth promoter of the present embodiment may further contain other components as long as the plant growth promoter exerts its growth promoting effect. For example, the plant growth promoter may contain water, humin, seaweed extract, polysaccharides, vitamins, minerals, plant-derived extract, and microbial extract. Humin is a type of humic substance, and refers to a black-brown or dark black organic fraction that is insoluble in neither alkali nor acid.

[0037] The total mass (%) of one or more kinds of amino acids relative to the total mass of humic acid in the plant growth promoter of this embodiment can be appropriately adjusted by a person skilled in the art depending on the type of plant, the growth method, etc., and may be, for example, 1% to 55%, 3% to 52%, 4% to 12%, 4% to 10%, 4% to 9%, 4% to 6%, or 5% to 10%. Here, when the humic acid is a humic acid extract, the TOC concentration of the humic acid extract is regarded as the concentration of humic acid to calculate the total mass. In addition, when one or more kinds of amino acids are provided as an enzymatic decomposition product or hydrolysate of raw materials derived from animals and plants, the total amount of amino acids contained in the enzymatic decomposition product or hydrolysate is used as the total mass of one or more kinds of amino acids. From the viewpoint of the plant growth promotion effect, for example, 4% to 9% is preferable for Brassicaceae plants, and 4% to 52% is preferable for Gramineae plants. From the viewpoint of the plant growth-promoting effect, when the one or more amino acids are casamino acids, the total mass (%) of the one or more amino acids relative to the total mass of humic acid is, for example, preferably 4% to 52%, and furthermore, in the case of soil application or foliar application (foliar spray), it is preferably 4% to 12%, and in the case of hydroponic cultivation, it is preferably 10% to 53%. From the viewpoint of the plant growth-promoting effect, when the one or more amino acids are soy peptone, the total mass (%) of the one or more amino acids relative to the total mass of humic acid is, for example, preferably 4% to 52%, and furthermore, in the case of soil application or foliar application (foliar spray), it is preferably 4% to 12%, and in the case of hydroponic cultivation, it is preferably 20% to 52%.

[0038] The proportion of casamino acid and / or soy peptone in the plant growth promoter according to this embodiment is not particularly limited, but may be, for example, 4 ppm to 800 ppm, or 40 ppm to 200 ppm, based on the total mass of the plant growth promoter.

[0039] In this specification, the phrase "containing as an active ingredient" means containing an amount that exerts a function, that is, an effective amount.

[0040] The plant growth promoter of the present embodiment may be expressed as a plant growth promoting composition.

[0041] The application amount and application period of the plant growth promoter of this embodiment are not particularly limited, and in the case of soil application and foliar application (foliar spray), the plant growth promoter can be applied once or more per crop season with the total organic carbon concentration of humic acid being up to 3000 mg per plant body, and in the case of hydroponic cultivation, the plant growth promoter can be applied with the total organic carbon concentration of humic acid being up to 500 mg / L. From the viewpoint of the plant growth promotion effect, in the case of soil application and foliar application (foliar spray), the plant growth promoter is preferably applied at 30 mL / 10a to 1000 mL / 10a, 300 mL / 10a to 500 mL / 10a, 350 mL / 10a to 450 mL / 10a, and particularly 400 mL / 10a.

[0042] The promotion of plant growth can be confirmed, for example, by an increase in leaf area, dry matter mass of above-ground and / or below-ground parts (roots), leaf length and / or root length, etc. The increase can be determined by comparing with a control. Here, the control means a plant treated under the same conditions except that humic acid and one or more amino acids are not applied.

[0043] Leaf area can be measured by image analysis using a photograph taken from directly above the plant. Leaf area can be calculated from a photograph using, for example, Image J or LIA for Win32 (LIA32) (freeware provided by Kazukiyo Yamamoto, Nagoya University, available from https: / / www.agr.nagoya-u.ac.jp / ~shinkan / LIA32 / ). When the leaf area is significantly increased (e.g., increased by 5% or more, increased by 10% or more, etc.) compared to the control, it may be determined that the plant growth is promoted.

[0044] The dry matter mass of the above-ground parts and / or underground parts (roots) can be measured by drying the harvested plant body in a thermostatic chamber at 80° C. for 8 hours or more. When the dry matter mass of the above-ground parts and / or underground parts is significantly increased (e.g., increased by 5% or more, increased by 10% or more) compared to the control, it may be determined that the plant growth is promoted.

[0045] The leaf length and / or root length can be measured by a conventional method. For example, if the leaf length and / or root length is significantly increased (e.g., increased by 5% or more, increased by 10% or more) compared to the control, it may be determined that the plant growth is promoted.

[0046] That the growth-promoting effect is superior to the application of humic acid alone may be judged by the degree of growth promotion, i.e., an increase in leaf area, dry matter weight of above-ground and / or below-ground parts (roots), leaf length and / or root length, etc., or by an increase in the persistence of the growth-promoting effect, or by both. The persistence of the growth-promoting effect may be judged to be improved when the number of days until the growth of the control is caught up is significantly longer (e.g., 1 day longer, 1.5 times longer, etc.) compared to the application of humic acid alone.

[0047] The plant growth promoter of the present embodiment can be produced by a method including mixing humic acid and one or more amino acids. In this production method, the method of mixing humic acid and one or more amino acids is not particularly limited, and for example, a humic acid extract and an enzymatic decomposition product or hydrolysate of an animal or plant-derived raw material containing one or more amino acids may be mixed. In addition, it may include adjusting the humic acid and one or more amino acids to an appropriate concentration by appropriately diluting with water or an aqueous solution before or after mixing. As the aqueous solution, for example, one containing fertilizer components such as urea, sodium nitrate, potassium nitrate, potassium hydroxide, potassium chloride, sodium phosphate, ammonium phosphate, or trace essential nutrients for plants can be used. In addition, the production method of the present invention may include preparing humic acid and one or more amino acids before mixing the humic acid and one or more amino acids.

[0048] As one embodiment, the present invention also provides a plant growth promoter comprising humic acid as an active ingredient in combination with one or more amino acids, wherein the humic acid has a melanic index of 2.0 or more, and the one or more amino acids are derived from an enzymatic decomposition product or hydrolysate of an animal or plant-derived raw material.

[0049] As a method of using it in combination with one or more amino acids, the plant growth promoter containing humic acid may be mixed with one or more amino acids before use, or the one or more amino acids may be applied before, after, or simultaneously with the application of the plant growth promoter containing humic acid.

[0050] There are no particular limitations on the amount and period of application of humic acid. In the case of soil application or foliar application (foliar spray), it can be applied at least once per crop season with a total organic carbon concentration of up to 3,000 mg per plant body, and in the case of hydroponic cultivation, it can be applied with a total organic carbon concentration of up to 500 mg / L.

[0051] The application amount and application period of one or more amino acids are not particularly limited. In the case of soil application and foliar application (foliar spray), they can be used in parallel with the application of humic acid, in a mixture or combination with humic acid, at least once per crop season. In the case of hydroponic cultivation, they can be used in parallel with the application of humic acid, in a mixture or combination with humic acid.

[0052] The total mass of the one or more amino acids relative to the total mass of humic acid is as described above.

[0053] The humic acid, one or more amino acids, and the target plant are as described above, and the above-mentioned embodiments can be applied as specific embodiments of the method without any restrictions.

[0054] As an embodiment of the present invention, a composition containing humic acid and one or more amino acids is also provided for use in promoting plant growth. Here, the melanic index of the humic acid is 2.0 or more, and the one or more amino acids are derived from an enzymatic decomposition product or hydrolysis product of an animal or plant-derived raw material. The same aspects as those of the plant growth promoter containing humic acid and one or more amino acids described above can be applied.

[0055] In addition, as one embodiment, the present invention also provides a use of a composition containing humic acid having a melanic index of 2.0 or more for promoting plant growth. This use is characterized by being used in combination with one or more amino acids, and the one or more amino acids are hydrolysates or enzymatic decomposition products of raw materials derived from animals and plants. The same aspects as those of the plant growth promoter containing humic acid, characterized by being used in combination with one or more amino acids, as described above, can be applied.

[0056] The humic acid, one or more amino acids, and the target plant are as described above, and the above-mentioned embodiments can be applied without limitation as specific embodiments of their use.

[0057] As an embodiment of the present invention, a concentrated liquid agent is also provided that, when diluted with water, constitutes the above-mentioned plant growth promoter containing humic acid and one or more amino acids, or a plant growth promoter containing humic acid. The dilution ratio with water is not particularly limited, but may be, for example, 100 times, 250 times, 1000 times, 5000 times, or 10000 times, and the concentration of each component in the concentrated liquid agent may be set so that the plant growth promoter is obtained when diluted to each dilution ratio. The proportion of humic acid in the concentrated liquid agent according to this embodiment is not particularly limited, but may be, for example, in the range of 0.5 to 50% based on the total mass of the concentrated liquid agent, and may be 40% or less, 30% or less, 20% or less, 10% or less, 5% or less, or 1% or less.

[0058] As one embodiment, the present invention also provides a kit for promoting plant growth, comprising humic acid and one or more amino acids, wherein the humic acid has a melanic index of 2.0 or more, and the one or more amino acids are derived from an enzymatic decomposition product or hydrolysate of an animal or plant-derived material.

[0059] In the kit of this embodiment, humic acid and one or more amino acids may be contained in different containers, humic acid and one or more amino acids may be contained in the same container, humic acid and one or more amino acids may be contained in different containers in a concentrated state, or humic acid and one or more amino acids may be contained in the same container in a concentrated state. That is, the kit of this embodiment may include a first container containing humic acid and a second container containing one or more amino acids, a first container containing humic acid and one or more amino acids, a first container containing concentrated humic acid and a second container containing concentrated one or more amino acids, or a first container containing concentrated humic acid and one or more amino acids. If concentrated, it is used at an appropriate concentration by diluting with water before application.

[0060] The humic acid may be contained as a humic acid extract, and one or more types of amino acids may be contained as an enzymatic decomposition product or hydrolysate of raw materials of animal or plant origin.

[0061] The kit of this embodiment may further include containers (second, third, and fourth containers) that contain water for dilution, additional components, and the like in addition to the above.

[0062] The container is not particularly limited, but for example, containers whose main material is polyethylene, polyethylene terephthalate, polypropylene, polystyrene, polycarbonate, etc. can be used.

[0063] The kit of this embodiment may be in a box containing instructions describing how to use the kit for use in promoting plant growth and / or may include instructions describing how to use the kit for use in promoting plant growth.

[0064] The humic acid, one or more amino acids, and the target plant are as described above, and the above-mentioned aspects can be applied as specific aspects of the kit without any restrictions.

[0065] In one embodiment, the present invention provides a method for promoting plant growth, comprising applying humic acid and one or more amino acids, wherein the humic acid has a melanic index of 2.0 or more, and the one or more amino acids are derived from an enzymatic decomposition or hydrolysis product of an animal or plant-derived material.

[0066] The application of humic acid and one or more amino acids may be carried out by mixing the plant growth promoter containing humic acid with one or more amino acids before use, or by applying the one or more amino acids before, after, or simultaneously with the application of the plant growth promoter containing humic acid.

[0067] There are no particular limitations on the amount and period of application of humic acid. In the case of soil application or foliar application (foliar spray), it can be applied at least once per crop season with a total organic carbon concentration of up to 3,000 mg per plant body, and in the case of hydroponic cultivation, it can be applied with a total organic carbon concentration of up to 500 mg / L.

[0068] The application amount and application period of one or more amino acids are not particularly limited. In the case of soil application and foliar application (foliar spray), they can be used in parallel with the application of humic acid, in a mixture or combination with humic acid, at least once per crop season. In the case of hydroponic cultivation, they can be used in parallel with the application of humic acid, in a mixture or combination with humic acid.

[0069] The total mass of the one or more amino acids relative to the total mass of humic acid is as described above.

[0070] The humic acid, one or more amino acids, and the target plant are as described above, and the above-mentioned embodiments can be applied as specific embodiments of the method without any restrictions. EXAMPLES

[0071] (humic acid) The humic acid used was a humic acid extract derived from lignite, and the TOC and MI were adjusted by adjusting the amount of nitric acid added during the production of the humic acid extract according to the method described in Patent No. 6231059. The humic acid used was a humic acid stock solution (humic acid extract derived from lignite, TOC 120000 ppm, MI 2.0 or more). The humic acid stock solution contains both humates and fulvic acids as humic acid, but contains more humates. In addition, the TOC of the humic acid stock solution is listed in ppm, but it is no problem to consider it to be almost the same value even if it is in mg / L.

[0072] (amino acid) Casamino acids (product number A1404HA) from Fujifilm Wako Pure Chemical Industries, Ltd. were used as casein hydrolysate. Soy peptone (product number 18889-95) from Nacalai Tesque, Inc. was used as soybean-derived enzymatic hydrolysate.

[0073] The results of measuring the total amino acids (%) and free amino acids (%) contained in casamino acids and soy peptone by amino acid analysis or HPLC are shown in Table 1. Tryptophan in casamino acids was not detected because it is decomposed during the manufacturing process. Free cysteine ​​in soy peptone was not detected because its content was below the detection limit.

[0074] [Table 1]

[0075] [Example 1 Foliar spray test] Test plant: Komatsuna (variety: Rakuten) In this cultivation test, the seedling soil was mixed with vermiculite and Akadama soil in a volume ratio of 1:1:1. The seedling soil used was manufactured by Takii Seeds Co., Ltd. and contained nitrogen 320, phosphoric acid 210, and potassium 300 mg / L.

[0076] Three to four seeds were sown in a 12 cm pot, and after seven days, one individual was selected. 12 pots were prepared for each test plot, and 14 days after sowing, 10 pots with a uniform growth stage were selected and sprayed on the leaves. At this time, materials were applied to plots other than the control plot, at a rate of 0.35 L / ha. The test plots for foliar spray and the conditions of application are shown below. The application rate was 100 μL per pot for all plots. The amount of irrigation was 88 L / ha.

[0077] [Table 2]

[0078] The total amino acid content (mass%) in humic acid is the value obtained by dividing the total amino acid content in casamino acids and soy peptone (calculated using the total amino acid ratios (51.6% and 59.6%) in Table 1) by the total mass of humic acid. The total mass of humic acid was calculated based on the TOC (470 ppm) of the humic acid stock solution, which was regarded as the humic acid concentration.

[0079] Photographs of the plants were taken from directly above on the 0th, 4th, 7th, and 12th day after application, and the leaf area (Leaf Area Index: LAI) was calculated. The results using casamino acids are shown in Figure 1, and the results using soy peptone are shown in Figure 2. In addition, 12 days after application, the above-ground parts were dissected and the leaf area was calculated, and the dry matter weight of the above-ground and below-ground parts was measured. The results using casamino acids are shown in Figure 3 and Table 3 below, and the results using soy peptone are shown in Figure 4 and Table 4 below. Leaf area was calculated from the photographs using LIA for Win32 (LIA32) (freeware provided by Kazukiyo Yamamoto of Nagoya University, available from https: / / www.agr.nagoya-u.ac.jp / ~shinkan / LIA32 / ).

[0080] [Table 3]

[0081] [Table 4]

[0082] When humic acid alone was applied, growth was vigorous compared to the control immediately after application, but growth eventually tended to catch up with that of the control. A certain degree of growth-promoting effect was also seen with casamino acids (CA) alone and soy peptone (SP) alone, but a synergistic growth-promoting effect was observed by combining humic acid with casamino acids (CA) or soy peptone (SP), and growth did not tend to catch up with that of the control (Figures 1 and 2). When sprayed on the leaves of komatsuna, the test plots of "humic acid + 1% casamino acids (CA)" and "humic acid + 1% soy peptone (SP)" showed a particularly significant increase in dry matter weight compared to the control (Figure 3, Table 3, Figure 4 and Table 4).

[0083] [Example 2 Hydroponic Cultivation Test] Test plant: Common wheat (variety: Norin 61) In this cultivation test, a 25% OAT-A formulation (OAT Agrio) with a pH adjusted to 5.60±0.01 was used. Wheat seeds were soaked in running water for about 6 hours, then vernalized on moistened filter paper in the dark at 10°C for 3 days, acclimated to cultivation conditions for about half a day to a day, and sown on 8-grain polyethylene mesh in each group. Five days after sowing, the plants were removed from the mesh, and leaf and root lengths, and the dry matter weight of the aboveground parts and roots were measured.

[0084] The cultivation conditions and results are shown in Tables 5 and 6 below. The value of the control area where only water was applied is set to 100. The percentage of soy peptone in Table 6 indicates the ratio of soy peptone to humic acid in the humic acid stock solution.

[0085] [Table 5]

[0086] [Table 6]

[0087] In hydroponic cultivation of wheat, it was found that the combination of humic acid concentrate and casamino acids promoted the growth of both above-ground and below-ground parts, especially when the casamino acids were 10 ppm and 40 ppm (Table 5). In addition, the combination of humic acid concentrate and soy peptone promoted the growth of above-ground parts in particular when the soy peptone was 5% and 10% relative to the TOC of the humic acid concentrate, and the plant height and above-ground dry matter mass increased (Table 6).

[0088] [Example 3 Foliar spray test] Test plant: Common wheat (variety: Kitahonami) This cultivation test was carried out at a test field in Iwamizawa City, Hokkaido.

[0089] The outline of the cultivation test division is shown in Figure 5, and foliar spraying was carried out at the wheat panicle formation stage (BBCH 31-35). The spraying conditions are shown in Table 7. In the spray material of "humic acid + soy peptone 1%", the total amino acid amount (mass%) relative to humic acid is 4.8%.

[0090] [Table 7]

[0091] The values ​​in Table 7 above are calculated as follows: Ward 1≒1.25×4.8=6m 2 <Test Area I, II, III> (7.5L / 10a) Materials: 30mL / 10a (0.03mL / m 2 ) =6m 2 ×0.03mL=0.18mL / test area Spray volume: 0.18mL x 250-fold dilution = 45mL / test area <Test area IV> (100L / 10a) Materials: 400mL / 10a (0.4mL / m 2 ) =6m 2 ×0.4mL=2.4mL / test area Spray volume: 2.4mL x 250-fold dilution = 600mL / test area

[0092] The results are shown in Table 8 below. The values ​​in parentheses are relative values, with the value for the control area, where only water was applied, taken as 100.

[0093] [Table 8]

[0094] In field spray tests on wheat, it was found that the combination of humic acid concentrate and soy peptone increased the number of ears. The effect of increasing yield was observed when the mixture of humic acid concentrate and soy peptone was applied at a rate of 30mL / 10a or more, and the yield increased especially when applied at a rate of 400mL / 10a.

Claims

1. A plant growth promoter comprising, as active ingredients, humic acid and one or more amino acids, The melanic index of the humic acid is 2 or more; A plant growth promoter, wherein one or more types of amino acids are derived from enzymatic decomposition or hydrolysis products of raw materials of animal or plant origin.

2. The plant growth promoter according to claim 1 , wherein the one or more types of amino acids are derived from an enzymatic hydrolysis product of a soybean-derived material or a hydrolysate of a milk protein.

3. The plant growth promoter according to claim 1, wherein the one or more types of amino acids include one or more types selected from two types of α-amino acids.

4. The plant growth promoter according to any one of claims 1 to 3, wherein the total mass of the one or more amino acids is 1% to 55% relative to the total mass of humic acid.

5. The plant growth promoter according to any one of claims 1 to 3, wherein the enzyme comprises papain.

6. The plant growth promoter according to any one of claims 1 to 3, wherein the humic acid comprises one or more selected from the group consisting of humic acid, fulvic acid, nitrohumic acid, potassium humate, ammonium humate, calcium humate, magnesium humate, ammonium nitrohumate, calcium nitrohumate, magnesium nitrohumate, potassium fulvic acid, ammonium fulvic acid, calcium fulvic acid, magnesium fulvic acid, ammonium nitrofulvic acid, calcium nitrofulvic acid, and magnesium nitrofulvic acid.

7. A method for producing the accelerator according to any one of claims 1 to 3, comprising mixing humic acid and one or more amino acids.

8. A plant growth promoter characterized by being used in combination with one or more amino acids, Contains humic acid with a melanic index of 2 or more as an active ingredient, A plant growth promoter, wherein one or more types of amino acids are hydrolysates or enzymatic decomposition products of raw materials derived from animals or plants.

9. The plant growth promoter according to claim 8 , wherein the one or more types of amino acids are derived from an enzymatic hydrolysis product of a soybean-derived material or a hydrolysate of a milk protein.

10. The plant growth promoter according to claim 8, wherein the one or more types of amino acids include one or more types selected from 20 types of α-amino acids.

11. The plant growth promoter according to any one of claims 8 to 10, wherein the total mass of the one or more amino acids is 1% to 55% relative to the total mass of humic acid.

12. A concentrated liquid preparation which constitutes the plant growth promoter according to any one of claims 1 to 3 or the plant growth promoter according to any one of claims 8 to 10 by dilution with water.

13. A plant growth promotion kit comprising humic acid and one or more amino acids, The melanic index of the humic acid is 2 or more; A kit, wherein one or more types of amino acids are hydrolysates or enzymatic decomposition products of raw materials derived from animals or plants.

14. A method for promoting plant growth, comprising applying humic acid and one or more amino acids, wherein the humic acid has a melanic index of 2 or more; The method, wherein the one or more amino acids are derived from an enzymatic decomposition or hydrolysis product of raw materials of animal or plant origin.

Images