Composition for scalp and / or hair

Abstract

To provide a composition for promoting hair growth.SOLUTION: Provided is a composition for scalp and / or hair, comprising one or more selected from Glycine max, rice bran, Scutellaria baicalensis Georgi (Labiatae), silk, Dioscorea villosa, Hippophae rhamnoides L., Curcuma, sake lees, and Prunus mume.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a composition to be applied to the scalp and / or hair. [Background technology]

[0002] New hair that grows from the hair root grows for a certain period of time, then stops growing and falls out, repeating this cycle. To keep hair healthy, it is important to maintain a normal hair cycle.

[0003] It is well known that aging causes the above hair cycle to not function normally, and hair thinning and hair loss progresses, but in recent years, due to the increase in social stress and the disturbance of lifestyle habits, even relatively young people have more and more cases of hair cycle not functioning normally, and hair thinning and hair loss progresses.In addition, in recent years, it has been revealed that not only aging but also obesity promotes hair thinning and hair loss (see Non-Patent Document 1).

[0004] Regarding thinning hair and hair loss, various methods for preventing and treating the condition have been proposed, and the development of effective hair growth agents and the like is underway (for example, Patent Document 1).

[0005] In addition, it is known that various moisturizing agents and oils are blended in hair growth agents to maintain the moisturizing feeling of the scalp and hair. However, there is a concern that some ingredients for maintaining the moisturizing feeling may make the scalp and hair sticky and cause a poor feeling of use. Therefore, development of hair growth agents that maintain a moisturizing feeling and have an excellent feeling of use is being promoted. For example, it has been disclosed that a hair growth agent blended with an extract of loquat leaves exhibits a hair growth effect and is not sticky when used (Patent Document 2). However, this technology is insufficient to exhibit an excellent moisturizing feeling and feeling of use, and further development of hair growth agents is required. [Prior art documents] [Patent documents]

[0006] [Non-Patent Document 1] http: / / www.qlifepro.com / news / 20210625 / obesity-accelerates-hair-thinning.html [Patent Document 1] Patent Publication No. 2021-172596 [Patent Document 2] JP 2010-105932 A Summary of the Invention [Problem to be solved by the invention]

[0007] An object of the present invention is to provide a novel composition that promotes hair growth. [Means for solving the problem]

[0008] The present inventors discovered that soybeans, rice bran, Scutellaria Root, silk, wild yam, seaberry, turmeric, sake lees and plum have the effect of activating SHH (Sonic hedgehog) signaling and that this effect promotes hair growth, thereby completing the present invention.

[0009] That is, the present invention is as follows. [1] A composition for the scalp and / or hair, comprising one or more selected from soybean, rice bran, scutellaria, silk, wild yam, seaberry, turmeric, sake lees and plum. [2] The composition described in [1], which is a hair growth composition. [3] The composition described in [1] or [2], characterized in that it is a composition for activating SHH signaling. [4] The composition described in any one of [1] to [3], which has a moisturizing effect. [5] The composition described in any one of [1] to [4], which is an external preparation. Effect of the Invention

[0010] The composition of the present invention promotes hair growth through the activation of SHH signaling. The composition of the present invention promotes hair growth through the activation of SHH signaling and also provides an excellent moisturizing effect. [Brief description of the drawings]

[0011] [Figure 1] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (soybean). [Diagram 2] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (rice bran). [Diagram 3] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (Scutellaria Root). [Figure 4] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition (silk) of the present invention. [Diagram 5] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (wild yam). [Figure 6] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (seaberry). [Figure 7] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (turmeric). [Figure 8] FIG. 2 is a graph showing the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (sake lees). [Figure 9] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (Prunus mume). [Figure 10] FIG. 2 is a graph showing the rate of change in stratum corneum moisture content for compositions of the present invention (soybean, rice bran, scutellaria, silk, wild yam, seaberry, turmeric, sake lees, and plum). DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] The composition of the present invention is applied to the scalp and / or hair, and is characterized by containing one or more selected from soybean, rice bran, Scutellaria root, silk, wild yam, seaberry, turmeric, sake lees, and plum (hereinafter, sometimes referred to as the components of the present invention). It is preferable that the composition of the present invention contains two or more components of the present invention in order to enjoy the effects of the composition more effectively.

[0013] The composition of the present invention promotes hair growth through activation of the SHH signal.

[0014] Hair follicles play an important role in hair growth, and hair follicles are maintained by the proliferation of hair follicle stem cells, which are the source of hair, and their differentiation into hair matrix cells. When SHH signaling is inhibited, hair follicle stem cells differentiate into epidermal cells and sebaceous glands, resulting in a decrease in the number of hair follicle stem cells. The composition of the present invention can activate SHH signaling, thereby inhibiting the decrease in the number of hair follicle stem cells and exerting a hair growth effect.

[0015] As mentioned above, obesity is a cause of thinning hair and hair loss (see Non-Patent Document 1), and since obesity suppresses the SHH signal, the composition of the present invention can activate the SHH signal and promote hair growth in thinning hair and hair loss caused by obesity.

[0016] Therefore, the composition of the present invention can be used as a hair growth composition, a composition for activating SHH signal, etc. The concept of hair growth in the present invention includes hair growth and hair care.

[0017] [Soybean] Soybeans are annual plants of the genus Glycine in the family Fabaceae, and examples thereof include black soybeans, red soybeans, green soybeans, white soybeans, Kurosaki brown soybeans, etc. As the part to be used in the present invention, seeds are preferred because they can more effectively exert the SHH signal activating effect, and because they can more effectively exert the SHH signal activating effect and also have excellent moisturizing effect.

[0018] Soybeans can be used, for example, as pulverized material, squeezed juice, extract, etc. Examples of pulverized material include dried powder and finely cut material. The squeezed juice and extract may be in liquid form, but can also be used in paste form or dried powder (squeezed juice powder, extract powder). In the present invention, an extract is preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The temperature of each solvent can be appropriately set at room temperature to below the boiling point.

[0019] [Rice bran] Rice bran is obtained by separating the pericarp, seed coat, germ, etc., which are produced when rice is polished. There is no particular limitation on the rice bran, but commercially available rice bran used as a raw material for cosmetics and food and beverages can be used. Rice bran may be in the form of an extract. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited, but hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), aqueous BG, etc. are preferred in terms of the effect of the present invention being more effectively achieved and ease of incorporation into the composition. The temperature of each solvent can be appropriately set within the range of room temperature to the boiling point or lower.

[0020] [Golden] Scutellaria baicalensis Georgi, Labiatae is a plant classified in the family Lamiaceae, and its scientific name is Scutellaria baicalensis Georgi, Labiatae. In the present invention, various parts of Scutellaria baicalensis can be used, but it is particularly preferable to use the root of Scutellaria baicalensis, because it can more effectively activate the SHH signal, and it can also more effectively activate the SHH signal and have excellent moisturizing properties.

[0021] Scutellaria root can be used, for example, as a pulverized product, a squeezed juice, an extract, etc. Examples of pulverized products include dried powder and finely cut products. The squeezed juice and extract may be in a liquid form, but can also be used in a paste form or a dry powder (juice powder, extract powder). In the present invention, an extract is preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The temperature of each solvent can be appropriately set at room temperature to below the boiling point.

[0022] [silk] Silk is an animal fiber derived from silkworm cocoon shells or silk threads, and is mainly composed of fibroin and sericin produced by silkworms. Silk can be used as an extract. In the present invention, the extract is preferred because it can better enjoy the effects of the present invention and can be easily incorporated into the composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can better enjoy the effects of the present invention and can be easily incorporated into the composition. The temperature of each solvent can be appropriately set between room temperature and the boiling point. Examples of silk extracts include silk peptides. Silk peptides are produced by hydrolyzing silk with chemicals such as alkaline substances, acids, neutral salts, and surfactants, or proteolytic enzymes. Fibroin and sericin are known as silk proteins, and the silk peptide of the present invention may be obtained by hydrolyzing silk containing both fibroin and sericin, or by purifying and then hydrolyzing one of the proteins.

[0023] [Wild yam] Wild yam is a plant classified in the genus Dioscorea of ​​the family Dioscoreaceae, and its scientific names are Dioscorea oppositifolia, Dioscorea opposita, and Dioscorea villosa. In the present invention, various parts such as leaves, stems, flowers, and roots can be used, but it is preferable to use the stems and roots, and it is particularly preferable to use the roots, because they can more effectively activate the SHH signal and can also exhibit excellent moisturizing effects.

[0024] Wild yam can be used, for example, as a pulverized product, a squeezed juice, an extract, etc. Examples of pulverized products include a dried powder and a finely shredded product. The squeezed juice and extract may be in a liquid form, but can also be used in a paste form or a dried powder (juice powder, extract powder). In the present invention, an extract is preferred because it can provide the effects of the present invention more effectively and can be easily incorporated into a composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can provide the effects of the present invention more effectively and can be easily incorporated into a composition. The temperature of each solvent can be appropriately set within a range from room temperature to the boiling point or lower.

[0025] [Seaberry] Seaberry is a plant classified in the genus Hippophae of the family Elaeagnaceae, and its scientific name is Hippophae rhamnoides L., and its Japanese name is Saji or Saji. In the present invention, various parts of the seaberry, such as the fruit, seeds, and leaves, can be used, but it is preferable to use the fruit, as it can more effectively activate the SHH signal and can also exhibit excellent moisturizing effects.

[0026] Seaberry can be used, for example, as a pulverized product, a squeezed juice, an extract, etc. Examples of pulverized products include a dried powder and a finely shredded product. The squeezed juice and extract may be in a liquid form, but can also be used in a paste form or a dried powder (juice powder, extract powder). In the present invention, an extract is preferred because it can better enjoy the effects of the present invention and can be easily incorporated into a composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can better enjoy the effects of the present invention and can be easily incorporated into a composition. The temperature of each solvent can be appropriately set within the range of room temperature to the boiling point or lower.

[0027] [Turmeric] Turmeric is a perennial plant belonging to the genus Curcuma of the Zingiberaceae family, and is also called turmeric. In the present invention, various parts such as leaves, flowers, and rhizomes can be used, but it is preferable to use rhizomes because they can more effectively activate the SHH signal, and because they can more effectively activate the SHH signal and have excellent moisturizing effects. Examples of types of turmeric include spring turmeric (Kyouou), autumn turmeric, and purple turmeric (Gajutsu), and autumn turmeric is preferred.

[0028] Turmeric can be used, for example, as a pulverized product, a squeezed juice, an extract, etc. Examples of pulverized products include a dried powder and a finely shredded product. The squeezed juice and extract may be in a liquid form, but can also be used in a paste form or a dried powder (squeezed juice powder, extract powder). In the present invention, an extract is preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The temperature of each solvent can be appropriately set at room temperature to below the boiling point.

[0029] [Sake lees] Sake lees are solids remaining after squeezing the fermented product (moromi) of raw materials such as rice and koji during the sake manufacturing process. Examples of sake lees include sake lees that are by-produced during sake manufacturing, as well as mirin lees and distilled sake lees (shochu lees, etc.). In the present invention, an extract is preferred because it can provide the effects of the present invention more effectively and can be easily incorporated into the composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can provide the effects of the present invention more effectively and can be easily incorporated into the composition. The temperature of each solvent can be appropriately set within the range of room temperature to the boiling point or lower.

[0030] [Plum] Prunus mume is a plant classified in the genus Prunus in the family Rosaceae, and its scientific name is Prunus mume. In the present invention, various parts of the plant, such as fruit, seeds, and leaves, can be used, but it is preferable to use fruit because it can more effectively activate the SHH signal and can also exhibit excellent moisturizing effects.

[0031] The type of plum tree is not particularly limited, and may be large plum, medium plum, small plum, etc., and examples thereof include Nanko plum, small plum Nanko, Furushiro, Uguisujuku, Aojiku, Shirakaga, Bungo, Beniei, Juro plum, Koshu small plum, Ryukyo small plum, etc.

[0032] Prunus mume can be used, for example, as a crushed product, a squeezed juice, an extract, etc. Examples of crushed products include a dried powder and a finely shredded product. The squeezed juice and extract may be in a liquid form, but can also be used in a paste form or a dried powder (juice powder, extract powder). In the present invention, an extract is preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The temperature of each solvent can be appropriately set at room temperature to below the boiling point.

[0033] The composition of the present invention can be produced by a known method by adding other ingredients, if necessary, in addition to the ingredients of the present invention. Examples of other ingredients include water, astringents, bactericides, antibacterial agents, ultraviolet absorbers, moisturizers, cell activators, anti-inflammatory agents, antioxidants, active oxygen removers, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, and plant extracts other than soybean, rice bran, Scutellaria baicalensis, silk, wild yam, seaberry, turmeric, sake lees, and plum.

[0034] The composition of the present invention can be used as an external preparation such as a medicine (including quasi-drugs) or a cosmetic. The external preparation is not particularly limited as long as it can be applied to the scalp and / or hair, and examples of the form include liquid, ointment, cream, gel, etc. Specific examples include shampoo, conditioner, rinse, treatment (including leave-in type), hair lotion, hair emulsion (hair cream), hair gel, hair cream, hair oil, hair pack, hair mask, hair tonic, hair liquid, hair spray, hair styling product, hair growth product, hair growth product, hair care product, etc.

[0035] The composition of the present invention can be used as a hair growth composition, and the hair growth composition is not particularly limited as long as it contains at least one of the components of the present invention and can be distinguished from other products in terms of being used for hair growth, and for example, the scope of the present invention includes products that display functions related to hair growth on the main body, packaging, instructions, or promotional materials (advertising media) of the product of the present invention. Specifically, examples of such products include, in addition to displays such as hair growth products (hair growth agents), hair growth products (hair growth agents), and hair care products (hair care agents), displays that include phrases such as "give hair firmness and strength," "for those who want firmness and strength," "improve scalp care effects," "care for scalp and hair," "prevent hair loss," "for those who are concerned about thinning hair," and "for healthy hair."

[0036] The composition of the present invention can be used as a composition for activating SHH signaling, and such a composition for activating SHH signaling is not particularly limited as long as it contains at least one of the components of the present invention and can be distinguished from other products in that it is used to activate SHH signaling, and for example, the scope of the present invention includes products that display a function related to SHH signaling activation on the main body, packaging, instructions, or promotional materials (advertising media) of the product of the present invention. Specifically, for example, display including the phrase "activates SHH signaling" can be exemplified.

[0037] The hair growth composition of the present invention may be one in which the ingredient of the present invention is indicated as an active ingredient, but is not limited to one in which the ingredient of the present invention is indicated as an active ingredient. For example, it may be one in which the active ingredient is not indicated. In addition, those manufactured and sold with the function and use suggested are included in the scope of the present invention. For example, there is a product sold with the experience testimonials of the person who took it, which mention the function and use as personal impressions, posted on the homepage, etc.

[0038] The content of the component of the present invention in the composition of the present invention (total amount when two or more kinds are used in combination) may be appropriately determined within the range in which the effect is exhibited. For example, the component of the present invention may be contained in the composition of the present invention in an amount of 0.0001 to 50 mass%, preferably 0.001 to 20 mass%, more preferably 0.01 to 20 mass%, and even more preferably 0.1 to 10 mass%, calculated as a dry mass. EXAMPLES

[0039] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

[0040] [Example 1] In order to confirm the hair growth effect of the composition of the present invention, the SHH signal activation effect of the components of the present invention was confirmed.

[0041] <Test substance> As for soybean, soybean seed extract obtained by extracting soybean seeds with aqueous BG was used. The rice bran used was a rice bran extract prepared by extracting rice seeds with aqueous BG. As the Scutellaria root, Scutellaria root extract obtained by extracting Scutellaria root with aqueous ethanol was used. As for silk, silkworm cocoon extract was used, which was extracted from silkworm cocoons with aqueous BG. As the wild yam, Dioscorea villosa root extract was used, which was extracted from the root of Dioscorea villosa with hydrated BG. As the seaberry, Hippophae rhamnoides fruit extract, which is extracted from seaberry fruit with hydrated BG, was used. The turmeric used was turmeric rhizome extract, which was prepared by extracting turmeric rhizomes with hydrated BG. The sake lees used were sake lees extract obtained by extracting sake lees with aqueous BG. As for the plum, plum fruit extract was used, which was prepared by extracting plum fruit with aqueous BG.

[0042] <Test Method> The test was conducted according to the following steps (1) to (5). (1) In a 37℃, 5% CO2 incubator, 75 cm 2 GliReporter-NIH3T3 cells (Funakoshi) were cultured in flasks using 10% CS-500 μg / mL Geneticine-DMEM medium.

[0043] (2) The cells were suspended by trypsinization and then transferred to a 75 cm 2 From flask to 96-well plate: 2.5 x 10 4 100 μL of cells were seeded to a cell density of 10 cells / well. 10% CS-DMEM was used as the medium.

[0044] (3) After overnight pre-incubation in a 5% CO2 incubator at 37°C, the medium was removed, and 50 μL of the prepared test substance solution serially diluted with the medium was added. As a control, medium without the test substance was also added. The medium used was 0.5% CS-1% NEAA-1 mM Na pyruvate-10 mM HEPES-OptiMEM. The test substance solution was filter sterilized before use.

[0045] (4) After culturing for 24 hours in a 37°C, 5% CO2 incubator, luciferase activity (SHH signal activity) was evaluated using a Luciferase Assay Kit (Applied Biological Materials).

[0046] (5) The relative luciferase activity value was calculated by setting the control luciferase activity value as 1.

[0047] The results are shown in Figures 1 to 9. 1 to 9, hibiscus, pueraria root, and capsicum all promoted luciferase activity (SHH signal activity). Therefore, the composition of the present invention has an SHH signal activating effect and can promote hair growth. [Example 2] In order to confirm the moisturizing effect of the composition of the present invention, the effect of the components of the present invention in increasing the moisture content of the stratum corneum was confirmed.

[0048] <Test substance> The soybeans, rice bran, scutellaria, silk, wild yam, seaberry, turmeric, sake lees, and plums used were the same as those used in Example 1. In addition, as the carrot extract, a ginseng root extract obtained by extracting ginseng root with aqueous ethanol was used.

[0049] <Test Method> (1) The insides of the forearms of three subjects were washed before application of the test substance.

[0050] (2) After leaving it to stand for 15 minutes, the moisture content of the stratum corneum at the planned application sites of the test substance on the inside of the forearms of the three subjects (each 2 cm long and 2 cm wide) was measured using the measuring device described below.

[0051] (3) 0.02 mL of the test substance was applied to each of the sites on the inside of the forearms of the three subjects, and after leaving the site for 15 minutes, the moisture content of the stratum corneum at the sites where the test substance was applied was similarly measured.

[0052] (4) The average value of the test substance application site after use of the skin topical agent was divided by the average value before use, and the resulting value was taken as the rate of change. The rate of change in stratum corneum moisture content is shown in Figure 10. In these figures, the higher the rate of change, the higher the evaluation.

[0053] The stratum corneum moisture content was measured using a Corneometer CM825 (Courage+Khazaka). This stratum corneum moisture content measurement device evaluates the moisture content of the stratum corneum as skin conductance (electrical conductivity, unit: μS). Measurements were performed using the standard method described in the instruction manual that came with the device.

[0054] As shown in Figure 10, soybean, rice bran, scutellaria root, silk, wild yam, seaberry, turmeric, sake lees and plum all increased the moisture content of the stratum corneum. Therefore, the composition of the present invention can increase the moisture content of the stratum corneum and exert a moisturizing effect.

[0055] [Composition Examples 1-1 to 1-5] (Manufacturing of hair growth products) A hair growth agent was produced comprising the components shown in Table 1 below. This hair growth agent exhibits the effects of the present invention.

[0056] [Table 1]

[0057] [Composition Examples 2-1 to 2-5] (Shampoo manufacturing) A shampoo was prepared from the components shown in Table 2 below. This shampoo exhibits the effects of the present invention.

[0058] [Table 2]

[0059] [Composition Examples 3-1 to 3-5] (Treatment manufacturing) A treatment was produced from each component listed in Table 3 below. This treatment exhibits the effects of the present invention.

[0060] [Table 3]

[0061] [Composition Examples 4-1 to 4-5] (Hair lotion manufacturing) A lotion was produced from each component shown in Table 4 below. This hair lotion exhibits the effects of the present invention.

[0062] [Table 4]

[0063] [Composition Examples 5-1 to 5-5] (Manufacture of hair lotion (hair milk)) A milky lotion was produced from each of the components shown in Table 5 below. This hair milky lotion exhibits the effects of the present invention.

[0064] [Table 5]

[0065] [Composition Examples 6-1 to 6-5] (Hair gel manufacturing) A gel was prepared from the ingredients listed in Table 6 below. This hair gel exhibits the effects of the present invention.

[0066] [Table 6]

[0067] [Composition Examples 7-1 to 7-5] (Hair cream manufacturing) A cream was prepared from the ingredients listed in Table 7 below. This hair cream exhibits the effects of the present invention.

[0068] [Table 7] [Industrial Applicability]

[0069] The composition of the present invention can be used as a hair growth agent and is therefore industrially useful.

Claims

1. A composition for the scalp and / or hair, comprising at least one selected from soybean, rice bran, scutellaria, silk, wild yam, seaberry, turmeric, sake lees and plum.

2. 2. The composition according to claim 1, which is a hair growth composition.

3. The composition according to claim 1, which is a composition for activating SHH signaling.

4. The composition according to any one of claims 1 to 3, which is an external preparation.

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