Production method of glucosinolate-containing composition and emulsification suppressing method of glucosinolate-containing composition

Abstract

To provide a production method of a glucosinolate-containing composition, achieving removal of contained oil and fat content or suppressing formation of an emulsification state while enhancing an extraction efficiency of glucosinolate from a glucosinolate-containing plant.SOLUTION: A production method of a glucosinolate-containing composition includes at least crushing and defatting steps in recovery of glucosinolate from the glucosinolate-containing composition, and also includes a fractionating step after the crushing step. In the crushing step, a glucosinolate-containing plant is crushed, and in the defatting step, the crushed glucosinolate-containing plant is defatted.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a method for producing a glucosinolate-containing composition and a method for inhibiting emulsification of a glucosinolate-containing composition. [Background technology]

[0002] Glucosinolates, which are unique components of cruciferous plants, are converted to isothiocyanates by myrosinase in the plant body and in the human intestinal flora, and are then absorbed into the body and exhibit a variety of physiological effects. Broccoli sprouts, in particular, contain a lot of sulfophalane glucosinolate (hereinafter referred to as "SGS"). The physiological effects of sulforaphane, which is converted from SGS, include detoxification, liver function improvement, and antioxidant effects. Focusing on this physiological effect, it is expected that glucosinolates, especially SGS, can be used in various food and beverage applications, which will contribute to the health of many consumers.

[0003] Patent Document 1 discloses a juice composition derived from a Brassicaceae plant, the purpose of which is to produce a juice having an increased amount of glucosinolates by heating kale under specific heating conditions and squeezing the juice.

[0004] US Patent No. 5,399,633 discloses a method for producing a food product containing a cruciferous plant, the object of which is to provide a food product containing significant amounts of phase II inducing potential and containing non-toxic concentrations of indole glucosinolates and their breakdown products and goitrogenic hydroxybutenyl glucosinolates, which comprises selecting seeds, germinating the seeds, harvesting the sprouts between the onset of germination and the wearing stage to produce the food product. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Patent No. 5726535 [Patent Document 2] JP 2000-502245 A [Patent Document 3] Special Publication No. 2012-500822 Summary of the Invention [Problem to be solved by the invention]

[0006] The problem in producing a glucosinolate-containing composition is to increase the extraction efficiency of glucosinolate from a glucosinolate-containing plant while increasing the suitability of the glucosinolate-containing composition for use. Isothiocyanate (Chemical formula 1) converted from glucosinolate is a useful component from the viewpoint of functionality, but is unstable due to its volatility and disappears over time. Therefore, when ingested, it is preferable that it is converted to isothiocyanate immediately before ingestion or in the body. Therefore, it is preferable that it is in the form of a highly stable glucosinolate in food and beverages or in compositions that are the raw materials for them.

[0007] [ka]

[0008] One method for producing a composition containing glucosinolate is to extract glucosinolate from a glucosinolate-containing plant.However, many glucosinolate-containing plants also contain myrosinase, which decomposes glucosinolate.Therefore, it is necessary to pay attention to the decomposition of glucosinolate by myrosinase during or after glucosinolate extraction.In addition, even after glucosinolate extraction, glucosinolate often remains in the glucosinolate plant.Therefore, there is room for further study of the method for extracting glucosinolate in order to improve extraction efficiency.

[0009] The present inventors have found that when producing a glucosinolate-containing composition from a glucosinolate-containing plant, the recovery efficiency of glucosinolate can be increased by crushing, squeezing, or extracting the glucosinolate-containing plant. On the other hand, they have found that when a glucosinolate-containing plant, particularly a seed, is crushed, squeezed, or extracted, the oil and fat content contained in the plant also migrates to the liquid portion. The oil and fat content causes the liquid portion to form an emulsion, making it difficult to use. In addition, the oil and fat content contains erucic acid, which is not preferable for consumption.

[0010] In other words, the objective of the present invention in producing a glucosinolate-containing composition is to increase the efficiency of extraction of glucosinolate from a glucosinolate-containing plant while removing the contained oil and fat components or inhibiting the formation of an emulsified state. [Means for solving the problem]

[0011] In order to solve the above problems, the present inventors have conducted extensive research and discovered that, in the recovery of glucosinolates, (1) crushing increases the recovery efficiency of glucosinolates, (2) crushing emulsifies the extract or juice, and (3) fats and oils reduce the recovery efficiency of glucosinolates and form an emulsified state. From these viewpoints, the present invention is defined as follows.

[0012] A first embodiment of the method for producing a glucosinolate-containing composition comprises at least the steps of disruption, defatting, and fractionation. It is the glucosinolate-containing plant that is disrupted by a person or an apparatus. It is the disrupted glucosinolate-containing plant that is defatted by a person or an apparatus. It is the defatted glucosinolate-containing plant that is fractionated by a person or an apparatus.

[0013] A second embodiment of the method for producing a glucosinolate-containing composition comprises at least the steps of defatting, disruption, and fractionation. It is the glucosinolate-containing plant that is defatty by a person or by a device. It is the defatted glucosinolate-containing plant that is disrupted by a person or by a device. It is the disrupted glucosinolate-containing plant that is fractionated by a person or by a device.

[0014] A third embodiment of the method for producing a glucosinolate-containing composition comprises at least the steps of crushing, fractionation, and defatting. The glucosinolate-containing plant is crushed by a person or an apparatus. The crushed glucosinolate-containing plant is fractionated by a person or an apparatus, and a fraction is obtained. The liquid fraction of the fraction is defatting by a person or an apparatus.

[0015] In the above-mentioned production method, the degreasing is preferably carried out using a liquid containing at least an organic solvent, or by centrifugation, and the fractionation is preferably carried out by solvent extraction or squeezing.

[0016] The method further comprises a heating step, in which the glucosinolate-containing plant is heated by a person or device, the heating being carried out prior to the crushing. The glucosinolate-containing plant is preferably in the form of a seed.

[0017] A first aspect of the method for inhibiting emulsification of a glucosinolate-containing composition comprises at least the steps of disruption, defatting, and fractionation. It is the glucosinolate-containing plant that is disrupted by a person or an apparatus. It is the disrupted glucosinolate-containing plant that is defatted by a person or an apparatus. It is the defatted glucosinolate-containing plant that is fractionated by a person or an apparatus.

[0018] A first aspect of the method for inhibiting emulsification of a glucosinolate-containing composition comprises at least the steps of defatting, disruption, and fractionation. It is the glucosinolate-containing plant that is defatting by a person or an apparatus. It is the defatted glucosinolate-containing plant that is disrupted by a person or an apparatus. It is the disrupted glucosinolate-containing plant that is fractionated by a person or an apparatus.

[0019] A second embodiment of the method for inhibiting emulsification of a glucosinolate-containing composition comprises at least the steps of disruption, fractionation, and defatting. The glucosinolate-containing plant is disrupted by a person or an apparatus. The disrupted glucosinolate-containing plant is fractionated by a person or an apparatus, and a fraction is thereby obtained. The liquid fraction of the fraction is defated by a person or an apparatus.

[0020] A third embodiment of the method for inhibiting emulsification of a glucosinolate-containing composition comprises at least the steps of disruption, defatting, and fractionation. It is the glucosinolate-containing plant that is disrupted by a person or an apparatus. It is the disrupted glucosinolate-containing plant that is defatted by a person or an apparatus. It is the defatted glucosinolate-containing plant that is fractionated by a person or an apparatus.

[0021] In the above method, the defatting is preferably performed using a liquid containing at least an organic solvent, or by centrifugation, and the fractionation is preferably performed by solvent extraction or squeezing.

[0022] The method further comprises a heating step, the heating being performed by a person or device on the glucosinolate-containing plant prior to the crushing, the glucosinolate-containing plant preferably being in the form of a seed. Effect of the Invention

[0023] The present invention makes it possible to increase the efficiency of extraction of glucosinolates from plants containing glucosinolates while removing the contained oils and fats or inhibiting the formation of an emulsified state. [Brief description of the drawings]

[0024] [Figure 1] Flowchart of a method for producing a glucosinolate-containing composition DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0025] <Glucosinolate-Containing Composition> The glucosinolate-containing composition according to the present invention (hereinafter referred to as "the composition") is a composition characterized by containing glucosinolate. Examples thereof include squeezed juice containing glucosinolate, plant extract containing glucosinolate, purified product containing glucosinolate, and concentrates, dried products, powders, etc.

[0026] <Glucosinolate> Glucosinolates are derivatives of glucose and amino acids, and are a group of organic compounds containing sulfur and nitrogen, as shown in the structural formula of Chemical Formula 2. Glucosinolates in the present invention are not particularly limited, but examples include sulforaphane glucosinolate (also called glucoraphanin), sinigrin, glucoerucin, glucobrassicin, glucoraphenin, glucoraphasatin, and phenethyl glucosinolate, and in the present invention, sulforaphane glucosinolate is particularly preferred. One or more of these glucosinolates may be used.

[0027] [ka]

[0028] <Glucosinolate-containing plants and cruciferous plants> The glucosinolate-containing plant of the present invention refers to a plant that contains glucosinolate. In particular, the glucosinolate-containing plant of the present invention is preferably a cruciferous plant. The cruciferous plant of the present invention refers to a plant that is classified into the cruciferous family. Examples include cabbage, broccoli, kale, watercress, komatsuna, bok choy, radish sprouts, cauliflower, Chinese cabbage, turnip, takana, kohlrabi, and the like. One or more of these plants may be used. In addition, the parts of these plants may be The whole or a part of the broccoli (flowers, leaves, or stems) may be used, and sprouts or seeds may also be used. In a particularly preferred embodiment of the present invention, broccoli sprouts or broccoli seeds are used.

[0029] The squeezed juice of a plant means the juice obtained by crushing and squeezing or straining the plant, the concentrated juice obtained by concentrating the squeezed juice, and the concentrated juice obtained by diluting and reconstituting the concentrated juice. These may further contain other ingredients (for example, small amounts of salt, spices, food additives, etc.).

[0030] A plant extract is a product obtained by extracting components contained in a plant using a solvent from the plant, and by concentrating the extracted components. The type of solvent is not particularly limited as long as it is a known solvent, and it may be hydrophilic or lipophilic, but since the solvent is used for food and beverage applications, it is preferable that the solvent is suitable for consumption.

[0031] <Method for Producing the Present Glucosinolate-Containing Composition> The method for producing the present food or beverage (hereinafter referred to as "the present method") mainly comprises a heating step, a crushing step, a defatting step, a fractionation step, a concentration step, a sterilization step, a filling step, and a cooling step.

[0032] <Heating (S010)> One of the purposes of heating is to inactivate myrosinase contained in the glucosinolate-containing plant. By carrying out the heating, myrosinase is inactivated and the decomposition of glucosinolate is inhibited. Another purpose of heating is sterilization. The timing of the heating is not particularly limited. One embodiment of the timing of heating is before the crushing step described below. By directly or indirectly heating the glucosinolate-containing plant, the myrosinase contained in the plant can be inactivated. Another embodiment of the timing of heating is at the same time as the fractionation described below. Furthermore, another embodiment of the timing of heating is at the same time as the concentration described below or after the concentration. The temperature during heating is not particularly limited. From the viewpoint of inactivating myrosinase, the lower limit of the temperature is preferably 60°C. More preferably, it is 70°C. Furthermore, the upper limit of the temperature is preferably 100°C.

[0033] <Crushing (S020)> The purpose of crushing is to increase the surface area of ​​the glucosinolate-containing plant. By crushing, the contact area of ​​the plant with the solvent increases in the fractionation described below, facilitating the transfer of glucosinolate to the solvent. From this viewpoint, it is preferable that the crushing is performed before or simultaneously with the fractionation described below. Another embodiment of the timing of crushing is after the heating. By crushing the glucosinolate plant after heating, the reaction by myrosinase after crushing can be suppressed. This makes it possible to suppress the decrease in glucosinolate.

[0034] The crushing may be performed in one step, or in two or more steps. The size of the plant after crushing is not particularly limited, but the preferred upper limit is 0.5 mm. The preferred lower limit is 10 μm. The crushing method is not particularly limited. Examples of crushing means include a hammer mill, a micrograder, a comitrol, a food processor, a colloid mill, etc.

[0035] <Degreasing (S030)> The purpose of degreasing is to remove fats and oils from the glucosinolate-containing plant. By removing fats and oils from the plant, the efficiency of recovering glucosinolates is increased. In addition, emulsification in the glucosinolate-containing composition obtained by recovering the glucosinolates is suppressed. The timing of degreasing is not particularly limited, but is preferably after the crushing. The crushing increases the surface area of ​​the glucosinolate-containing plant, thereby increasing the efficiency of degreasing.

[0036] The method of degreasing is not particularly limited. One specific method of degreasing is to use an organic solvent. The organic solvent used here is preferably hexane or a mixture of hexane and ethanol. The mixture ratio of hexane and ethanol is preferably XX to XX. Another specific method of degreasing is centrifugation. When the composition to be degreased is a mixture of an aqueous medium and an oily medium, it is possible to separate the aqueous part and the oily part due to the difference in specific gravity by subjecting the composition to a centrifugation treatment.

[0037] <Fractionation (S040)> In the present invention, the purpose of fractionation is to recover glucosinolates. Specific examples of fractionation include extraction, squeezing, and the like.

[0038] <Extraction (S041)> The purpose of extraction is to extract glucosinolates from a glucosinolate-containing plant. When the glucosinolate-containing plant is bathed in an extraction solvent, the glucosinolates dissolve in the extraction solvent. By increasing the temperature of the extraction solvent, the extraction time can be shortened. It is the liquid portion that is recovered by this extraction. Furthermore, by increasing the temperature of the extraction solvent, myrosinase in the glucosinolate-containing plant can be inactivated. From this perspective, the temperature when extracting glucosinolates from the plant is 60°C or higher and 95°C or lower, and preferably 70°C or higher and 95°C or lower.

[0039] <Extraction Solvent> In the present invention, the extraction solvent used during the extraction is a liquid containing at least water. An organic solvent is permitted to be contained as the extraction solvent in addition to water. The organic solvent is preferably a lower alcohol. Furthermore, the lower alcohol is preferably ethanol. The weight ratio of ethanol in the extraction solvent is preferably 1% by weight or more and 20% by weight or less. More preferably, it is 5% by weight or more and 20% by weight or less.

[0040] <Squeezing (S042)> Squeezing the crushed glucosinolate-containing plant produces juice and a cake. Squeezing can also be performed after adding an aqueous solvent such as water to the glucosinolate-containing plant. The liquid portion is recovered by squeezing. The method for squeezing the glucosinolate-containing plant may be a known method, such as squeezing or centrifugal separation. Examples of juice extracting devices include an extruder, a filter press, a decanter, a ginner, etc.

[0041] <Concentration (S050)> The purpose of concentration is to improve the handling of the composition. By concentrating a liquid composition, the volume of the liquid is reduced. In other words, the storage cost of the liquid is reduced. The concentration method may be a known method, such as vacuum concentration, membrane concentration, freeze concentration, etc.

[0042] <Sterilization, cooling (S060), filling (S070)> In addition to the above, the present manufacturing method appropriately employs sterilization, filling, and cooling. The sterilization method may be a known method, such as plate-type sterilization and tubular-type sterilization. The cooling method may be a known method. The filling method may be a known method. The container into which the present food or beverage is filled (packed) may be a known one, such as a can, a bottle, a paper container, or a polyethylene container.

[0043] <Glucosinolate-containing food and beverage> The glucosinolate-containing food and beverage according to the present invention (hereinafter referred to as "this food and beverage") refers to a beverage or food containing glucosinolate. It is preferable that this food and beverage contains, as a raw material, the glucosinolate-containing composition according to the present invention. The beverage is not particularly limited as long as it is generally recognized as a beverage such as a soft drink or a smoothie. This food and beverage includes those containing some solid components such as soup.

[0044] <Physiological activity> The functional properties of glucosinolate in this glucosinolate-containing food and beverage are enhanced. From this perspective, this food and beverage may have at least one functionality selected from the group consisting of liver function improvement effect, blood glucose level improvement effect, diabetes prevention effect, Helicobacter pylori eradication effect, vital capacity and respiratory function improvement effect, LDL cholesterol reduction effect, promotion effect of excretion of air pollutants, bowel movement improvement effect, memory and attention improvement effect, schizophrenia alleviation effect, obesity prevention and improvement effect, fatty liver prevention and improvement effect, dementia prevention effect, cognitive function improvement effect, intestinal flora improvement effect, depression prevention effect, prostate prevention effect.

Examples

[0045] The test examples 1 to 5 are those that embody this food and beverage. However, the scope of the claims according to the present invention is not limited by these examples.

[0046] <Measurement of SGS concentration> The measurement method of SGS adopted in this measurement is the HPLC method. The sample was appropriately diluted and filtered through a filter and used as the specimen. The detailed measurement conditions are as follows.

[0047] <HPLC measurement conditions> Instrument: ACQUITY UPLC H-Class system (manufactured by Waters) Column: ACQUITY CSH C18 (Φ2.1×100 mm, 1.7 μm) (manufactured by Waters) Column temperature: 30 °C Sample injection volume: 10 μL Mobile phase A: Ultra-pure water: Trifluoroacetic acid = 99.95:0.05 (v:v) Mobile phase B: Methanol: Trifluoroacetic acid = 99.95:0.05 (v:v) Gradient: Maintain 0% of mobile phase B for 5 minutes, linear gradient from 0% to 10% of mobile phase B in 10 minutes, linear gradient from 10% to 100% of mobile phase B in 5 minutes, maintain 100% of mobile phase B for 5 minutes, linear gradient from 100% to 0% of mobile phase B in 2 minutes, maintain 0% of mobile phase B for 5 minutes Flow rate: 0.1 mL / min Detection wavelength: 235 nm

[0048] <SGS recovery rate> In this example, the SGS recovery rate indicates the ratio of the SGS concentration of the sample in each test example when the SGS concentration of the sample in Comparative Example 1 is set to 100. The numerical values shown in Table 1 and Table 2 are all average values at n = 2.

[0049] <Confirmation of emulsification state> In this example, the emulsification state of each test example was confirmed by the measured value of the absorbance at 660 nm (OD660nm). The higher the value of OD660nm, the greater the turbidity, indicating that emulsification has occurred.

[0050] [Test 1] In Test 1, the effects of crushing broccoli seeds on the SGS extraction efficiency and the effects of degreasing treatment with hexane on the SGS extraction efficiency were confirmed.

[0051] <Comparative Example 1> Commercially available broccoli seeds (sold by Plant Life Design) were heat-treated at 90°C for 10 minutes in an autoclave. 2 g of the autoclaved seeds were mixed with 10 g of water, and heated in a thermostatic bath at 70°C for 60 minutes while stirring. After heating, the seeds were removed, and the liquid portion was recovered and the SGS concentration of the liquid portion was measured. Additionally, the SGS recovery rate was calculated based on the SGS concentration of the liquid portion and the amount of the recovered liquid portion.

[0052] <Test Example 1> Commercially available broccoli seeds (sold by Plant Life Design) were heat-treated at 90°C for 10 minutes in an autoclave. 2 g of the autoclaved seeds were crushed in a food processor, mixed with 10 g of water, and heated in a thermostatic bath at 70°C for 60 minutes while stirring. After heating, the seeds were removed and the liquid portion was recovered, and the SGS concentration of the liquid portion was measured. Additionally, the SGS recovery rate was calculated based on the SGS concentration of the liquid portion and the amount of the recovered liquid portion.

[0053] <Test Example 2> Commercially available broccoli seeds (sold by Plant Life Design) were heat-treated at 90°C for 10 minutes in an autoclave. 2 g of the autoclaved seeds were degreased by stirring with three times the amount of hexane for 1 hour. The degreased seeds were dried, mixed with 10 g of water, and heated at 70°C for 60 minutes in a thermostatic bath while stirring. After heating, the seeds were removed, and the liquid portion was recovered and the SGS concentration of the liquid portion was measured. Additionally, the SGS recovery rate was calculated based on the SGS concentration of the liquid portion and the amount of the recovered liquid portion.

[0054] <Test Example 3> Commercially available broccoli seeds (vendor: Plant Life Design) were heat-treated in an autoclave at 90 °C for 10 minutes. 2 g of the seeds after autoclaving were defatted by stirring with three times the amount of hexane for 1 hour. The defatted seeds were dried and then crushed in a food processor. 10 g of water was mixed with the crushed seeds, and the mixture was heated with stirring at 70 °C for 60 minutes using a thermostatic bath. After heating, the seeds were removed, the liquid portion was recovered, and the SGS concentration of the liquid portion was measured. Additionally, based on the SGS concentration of the liquid portion and the amount of the recovered liquid portion, the SGS recovery rate was calculated.

[0055] <Evaluation of SGS Extraction Efficiency Improvement> In this test, the category with a higher SGS recovery rate compared to Comparative Example 1 was rated as "〇" for the evaluation of SGS extraction efficiency improvement. Also, the category with a lower SGS recovery rate compared to Comparative Example 1 was rated as "×" for the evaluation of SGS extraction efficiency improvement.

[0056]

Table 1

[0057] [Test 2] In Test 2, the effect of defatting treatment on the emulsification state of the extraction sample was confirmed. The broccoli seeds used were from a different lot than those used in Test 1.

[0058] <Test Example 4> Commercially available broccoli seeds (vendor: Plant Life Design) were heat-treated in an autoclave at 90 °C for 10 minutes. 2 g of the seeds after autoclaving were crushed in a food processor, 10 g of water was mixed, and the mixture was heated with stirring at 70 °C for 60 minutes using a thermostatic bath. After heating, the seeds were removed, the liquid portion was recovered, and the SGS concentration of the liquid portion was measured. Additionally, OD660nm was measured to determine the emulsification state.

[0059] <Test Example 5> Commercially available broccoli seeds (sold by Plant Life Design) were heat-treated at 90°C for 10 minutes using an autoclave. 2g of the autoclaved seeds were crushed in a food processor. The crushed seeds were stirred with three times the amount of hexane for 1 hour, and this was performed three times to perform a degreasing treatment. The degreasing-treated seeds were dried, and then mixed with 10g of water, and heated at 70°C for 60 minutes while stirring using a thermostatic bath. The seeds were removed after heating, and the liquid portion was collected, and the SGS concentration of the liquid portion was measured. In addition, OD660nm was measured to measure the emulsification state.

[0060] [Table 2]

[0061] <Results and Summary> When extracting SGS from broccoli seeds, crushing or degreasing improved the recovery rate of SGS. In particular, the improvement in SGS recovery rate by crushing was remarkable. On the other hand, it was confirmed that when broccoli seeds were crushed and SGS was extracted, the sample emulsified. Here, it was found that crushing and degreasing reduced turbidity and suppressed emulsification.

[0062] From the above test results, it was found that crushing or degreasing is effective for increasing the efficiency of glucosinolate extraction when extracting glucosinolate from glucosinolate plants. It was also found that crushing and degreasing can increase the efficiency of glucosinolate extraction while suppressing the emulsification state. [Industrial Applicability]

[0063] An area in which the invention is useful is methods for producing glucosinolate-containing compositions and methods for increasing the efficiency of extraction of glucosinolates from glucosinolate-containing plants.

Claims

1. A method for producing a glucosinolate-containing composition comprising at least the following steps: degreasing, in which the glucosinolate-containing plant is defatted; disruption, in which the defatted glucosinolate-containing plant is disrupted; and fractionation, in which the disrupted glucosinolate-containing plant is fractionated.

2. A method for producing a glucosinolate-containing composition comprising at least the following steps: disruption, in which a glucosinolate-containing plant is disrupted; fractionation, in which the disrupted glucosinolate-containing plant is fractionated, thereby obtaining a fraction; and delipidation, in which the fraction is delipidated.

3. 3. The manufacturing method according to claim 1 or 2, wherein the degreasing is performed using a liquid containing at least an organic solvent.

4. The method according to claim 2, wherein the degreasing is performed by centrifugation.

5. The method according to any one of claims 1 to 4, wherein the fractionation is performed by solvent extraction or squeezing.

6. The method of any one of claims 1 to 5 further comprising the steps of: heating, wherein the glucosinolate-containing plant is heated, said heating being performed prior to said crushing.

7. 7. The method according to claim 1, wherein the glucosinolate-containing plant is in the form of a seed.

8. A method for inhibiting emulsification of a glucosinolate-containing composition comprising at least the following steps: degreasing, in which the glucosinolate-containing plant is degreased; disruption, in which the defatted glucosinolate-containing plant is disrupted; and fractionation, in which the disrupted glucosinolate-containing plant is fractionated.

9. A method for inhibiting emulsification of a glucosinolate-containing composition comprising at least the following steps: disruption, in which a glucosinolate-containing plant is disrupted; fractionation, in which the disrupted glucosinolate-containing plant is fractionated, thereby obtaining a fraction; and defatting, in which the fraction is defated.

10. 10. The method according to claim 8 or 9, wherein the degreasing is carried out using a liquid containing at least an organic solvent.

11. The method of claim 9, wherein the defatting is performed by centrifugation.

12. 12. The method of any one of claims 8 to 11, wherein the fractionation is by solvent extraction or squeezing.

13. 13. The method of any of claims 8 to 12, further comprising the steps of: heating, wherein it is the glucosinolate-containing plant that is heated, said heating occurring prior to said crushing.

14. 14. The method of any one of claims 8 to 13, wherein the glucosinolate-containing plant is in the form of a seed.

Images