Supplement for improving saccharification and method for manufacturing the same

Abstract

To provide a novel supplement effective in improving saccharification.SOLUTION: A supplement for improving saccharification contains a pressurized enzyme decomposer obtained by heat treatment of oyster at 60°C or above, followed by enzymatic reaction under pressure.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a glycation improving supplement and a method for producing a glycation improving supplement. [Background technology]

[0002] Foods have a physical condition-regulating effect known as food functionality (tertiary function), and are said to be effective in preventing and improving disease. In particular, foods that clearly have this effect are called health functional foods (foods for specified health uses, foods with nutrient functions), and as foods for specified health uses and foods with nutrient functions have been highlighted, the need for knowledge about food functionality is increasing. In this regard, zinc, one of the components of foods with nutrient functions, is not only used as a treatment for zinc deficiency, but is also thought to be necessary for producing the hormone (insulin) that regulates blood sugar, as has been reported in recent years in relation to diabetes, and it is expected that its scope of application will expand beyond the "treatment" of diseases to include "prevention." [Prior art documents] [Patent documents]

[0003] [Patent Document 1] JP 2005-82482 A Summary of the Invention [Problem to be solved by the invention]

[0004] The present invention has been made in consideration of the above circumstances, and aims to provide a novel supplement that is effective in improving glycation. [Means for solving the problem]

[0005] As a result of intensive research conducted by the present inventors to achieve the above object, it was found that a pressurized enzymatic hydrolyzate of oysters, which was obtained by heating oysters at 60°C or higher and then reacting the oysters with an enzyme under pressure, has excellent HbA 1CIt was found that the compound has an improving effect and is effective as a supplement for improving glycation, which led to the completion of the present invention.

[0006] That is, the present invention relates to a glycation improving supplement containing a pressurized enzymatic hydrolysate obtained by heat-treating oysters at 60°C or higher and then subjecting them to an enzymatic reaction under pressure.

[0007] The present invention also relates to a method for producing oysters, comprising the steps of: heat treating oysters at 60°C or higher; The present invention relates to a method for producing a glycation improving supplement, which includes a step of subjecting the obtained heat-treated product to an enzymatic reaction under pressure.

[0008] Furthermore, the present invention relates to the use of a pressurized enzymatic hydrolysate obtained by heat-treating oysters at 60°C or higher and then subjecting them to an enzymatic reaction under pressure as a supplement for improving glycation. Effect of the Invention

[0009] According to the present invention, a supplement that is effective in improving glycation can be provided. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] The present invention will be described in detail below. The present invention is a glycation improving supplement that contains a pressurized enzymatic hydrolysate obtained by heat-treating oysters at 60°C or higher and then subjecting them to an enzymatic reaction under pressure.

[0011] There are no particular limitations on the place of origin or type of oysters, and one type may be used, or two or more types may be mixed. In addition, either raw or frozen oysters may be used. The raw material may be finely chopped to make it easier to process.

[0012] Before the enzymatic reaction under pressure, the oysters are heat-treated at 60°C or higher, preferably 80°C or higher. This heat treatment can inactivate the autolytic enzymes in the oysters. The upper limit of the heating temperature is not particularly limited, but can be 100°C or lower. The heat treatment time is about 1 to 120 minutes, preferably 20 to 60 minutes.

[0013] Conditions for reacting the heat-treated product obtained above with an enzyme under pressure are preferably a pressure of 40 to 200 MPa, more preferably 50 to 150 MPa, and even more preferably 60 to 100 MPa. Furthermore, it is preferable to apply pressure and heat, and the temperature is preferably 20 to 90°C, more preferably 30 to 80°C, even more preferably 40 to 70°C, and particularly preferably 40 to 60°C. The enzyme reaction time is preferably 1 to 72 hours, more preferably 1 to 48 hours, even more preferably 3 to 48 hours, and particularly preferably 3 to 36 hours, 3 to 24 hours, and 6 to 24 hours in that order. The pressurizing device is not particularly limited, and for example, a pressurizing / heating processor or the like is suitable.

[0014] The enzyme is not particularly limited, but examples thereof include proteases, carbohydrate decomposition enzymes, etc., with proteases being preferred. Examples of proteases include known proteases such as papain, pepsin, trypsin, and chymotrypsin, and proteases produced by various microorganisms. Among these, thermolysin, papain, and pancreatin are preferred, with thermolysin being particularly preferred.

[0015] The amount of the enzyme is not particularly limited and is selected depending on the characteristics of each enzyme, but is preferably 0.01 to 5.0% by mass, more preferably 0.1 to 0.5% by mass, based on the target of enzyme treatment.

[0016] The enzyme can be inactivated according to the method for inactivating the enzyme used, for example, by treating at 80° C. or higher for 10 to 30 minutes.

[0017] The obtained pressurized enzymatic hydrolyzate of oysters can then be subjected to a known treatment process such as filtration, etc. The pressurized enzymatic hydrolyzate may be in a liquid state, and may be made into a solid state by appropriately performing concentration, separation and purification, spray drying, etc.

[0018] [Method of producing pressurized enzymatic hydrolysate of oysters] The method for producing the pressurized enzymatic hydrolysate includes the steps of heat-treating oysters at 60°C or higher and reacting the obtained heat-treated product with enzymes under pressure, as described above.

[0019] [Supplement for improving glycation] The glycation improving supplement of the present invention contains the above-mentioned oyster pressurized enzyme decomposition product as an active ingredient, and can be taken by oral administration or external application. The intake amount of the supplement is, for example, about 300 mg per capsule (content about 240 mg (including about 216 mg of oyster powder)), and a maximum of 20 capsules per day per adult is recommended, preferably about 3 to 10 capsules per day. In this case, the weight of zinc taken into the body is only about 1.1 mg to 3.6 mg in total even with 10 capsules, so in the case of Japanese people, the tolerable upper limit of zinc is set at 40 mg per day for men aged 18 to 29, 45 mg for men aged 30 to 64, 40 mg for men aged 65 or over, 35 mg for women aged 18 to 74, and 30 mg for women aged 75 or over (Dietary Reference Intake). In light of this, it can be said that the risk of health damage such as copper deficiency, anemia, and stomach problems due to excessive intake is almost no problem. In particular, many women trying to conceive recognize the importance of folic acid, but are not aware that zinc is necessary for fertility and fetal health, or that they are zinc deficient themselves. In recent years, it has become possible to check zinc levels in blood for those suspected of zinc deficiency, so zinc checks are likely to be implemented at fertility treatment sites. However, even if there is zinc deficiency, drugs covered by insurance are difficult for those trying to conceive to use, so this supplement can be safely taken by those trying to conceive, pregnant women, and those with polycystic ovarian disorder, who are often deficient in minerals such as zinc, which are common among Japanese people. In safety tests, it was confirmed that the glycation-improving supplement of the present invention poses no health problems even when taken at a daily intake of 20 capsules, and as described below, in terms of improving glycation, HbA 1CIt was confirmed that the composition can exert a very sufficient effect on the reduction of the values ​​of . The amount of the pressurized enzymatic hydrolyzate of oysters in the composition is not particularly limited, and is appropriately selected from the range of 0.01 to 99% by mass so as to achieve the above-mentioned intake amount. The method and frequency of intake are also not particularly limited, and the desired effect can be obtained by taking several capsules at once or by taking them in several divided doses per day.

[0020] Glycation improvement supplements include quasi-drugs, etc., as well as foods, functional foods, foods for specified health uses, etc. There are no particular limitations on the dosage form, and in addition to capsules (animal and vegetable), granules, fine granules, powders, tablets, liquids, emulsions, suspensions, and other oral preparations, beverages, solids, semi-solids, jellies, and other food forms, and topical preparations such as lotions, ointments, and poultices can be used.

[0021] The glycation improving supplement of the present invention may contain any optional ingredient that is usually used in the above-mentioned dosage forms in normal amounts, as long as the effect of the present invention is not impaired.

[0022] In view of the above embodiment, the present application also includes the following inventions. [1] A glycation-improving supplement containing pressurized enzymatic hydrolysates obtained by heating oysters at 60°C or above and then subjecting them to an enzymatic reaction under pressure. [2] The glycation improving supplement according to [1], wherein the enzymatic reaction is a pressurized enzymatic reaction at a pressure of 40 to 200 MPa and a temperature of 30 to 80°C for 1 to 48 hours. [3] The glycation improving supplement according to [2], wherein the enzyme reaction is a pressurized enzyme reaction at a pressure of 50 to 150 MPa and a temperature of 40 to 70°C for 3 to 24 hours. [4] A glycation improving supplement according to any one of [1] to [3], wherein the enzyme is a protease. [5] A glycation improvement supplement according to [4], wherein the enzyme is thermolysin. [6] A process of heat treating the oysters at 60°C or higher; A method for producing a glycation improving supplement includes a step of subjecting the obtained heat-treated product to an enzymatic reaction under pressure. [7] Use of pressurized enzymatic hydrolysate obtained by heating oysters at 60°C or higher and then subjecting them to an enzymatic reaction under pressure as a supplement for improving glycation. EXAMPLES

[0023] The present invention will be described in more detail below with reference to the following examples, but the present invention is not limited to the following examples.

[0024] (Oyster extract) Product: Oyster pressure enzyme hydrolysis extract After crushing the frozen raw oysters, the autolytic enzymes were inactivated by keeping the temperature at 90℃ or higher in a steam boiler agitator. Protease was added to the mixture, and the mixture was subjected to a pressurized, heated enzymatic decomposition process in a Pascal-kun (manufactured by Hiroshima Yanmar Shoji Co., Ltd.). The added enzymes were then inactivated by keeping the temperature at 90℃ or higher, and a pressure enzymatic decomposition extract of oysters was obtained. Comparison product: Oyster hot water extract Frozen raw oysters were semi-thawed and subjected to hot water treatment, after which the oyster meat was removed and the extract was heated and concentrated to obtain a hot water extract of oysters.

[0025] (First evaluation) Fifteen adult males aged 20 to 66 years (mean age 43.9±14.8 years) with an average weight of 68.3kg±11.3kg (BMI: 23.8±3.4) were administered 20 capsules of the present invention supplement, which contains approximately 140mg of oyster extract powder per 100g of oyster powder (approximately 6mg zinc per day), for three months. 1C It was confirmed that the HbA 1C A normal value is less than 5.6%, and a normal value is HbA 1C In addition, in the above subjects, fasting blood glucose changed from 96.4±7.3 to 93.7±9.0, with no significant difference, and no hypoglycemic symptoms were observed. In addition, no other adverse events were observed.

[0026] (2nd evaluation) Twenty-six adults (11 men and 15 women) with mildly impaired glucose tolerance were randomly divided into two groups. Thirteen patients in group A (10 with borderline diabetes and 3 with type 2 diabetes; age 61.8±11.8, mean weight 60.4±13.5, BMI: 23.6±3.4) were not administered oyster powder and were observed with their usual treatment. Thirteen patients in group B (7 with borderline diabetes and 6 with type 2 diabetes; age 64.3±7.3, mean weight 62.8±1.0, BMI: 24.8±2.7) were administered oyster powder (10 capsules / day), equivalent to approximately 3 mg of zinc per day, for three months. When comparing groups A and B, no significant difference was found in the clinical test data in group A, and in group B, the fasting blood glucose level before supplement administration was 107.7±28.7, and after administration it was 105.2±24.6, showing no significant difference. Meanwhile, HbA 1C It was confirmed that the blood glucose level significantly decreased from 5.83±0.72 before the supplement was administered to 5.60±0.84 after administration (p<0.01). This suggests that the supplement of this invention has the potential to significantly improve postprandial blood glucose levels. There were no significant differences in BMI or other biochemical test data, and no adverse events were observed.

[0027] (Third evaluation) Oyster powder (5 capsules / day) equivalent to approximately 1.5 mg of zinc per day was administered to 49 healthy subjects (21 men and 28 women, average age 44.6±11.8) who were engaged in normal daily activities and work for three months. As with the above evaluation, HbA 1C It was confirmed that there was a significant improvement in fasting blood glucose levels, but no significant difference was observed in fasting blood glucose levels. This shows that the pressurized and enzymatically hydrolyzed oysters of this example are sufficiently effective as a supplement for improving glycation even at 5 capsules per day. In addition, administration tests of the pressurized and enzymatically hydrolyzed oysters of this example were conducted not only on patients with abnormal glucose metabolism, but also on healthy individuals, and it was revealed that they can be used safely without causing hypoglycemia symptoms.

[0028] Although the supplement of the present invention contains a much smaller amount of minerals than general multi-mineral supplements (e.g., about 1.5 mg / day of zinc equivalent) because it is made from natural materials, the reason why many mineral data have improved is that the whole shucked oyster meat is enzymatically decomposed, resulting in some parts being amino acid chelated minerals, which are easily absorbed, and therefore significant effects have been obtained despite the small mineral content. In addition, the reason why HbA1c has improved is thought to be due to the significant increase in amylase and lipase, which improves postprandial glycation stress and blood sugar spikes. The supplement of the present invention has not caused any adverse events to date and is safe and secure, and can be used to improve basic pregnancy strength and to supplement minerals for pregnant women, and as a result, it is thought to contribute to fetal health.

[0029] Although several embodiments of the present invention have been described above, these embodiments are presented as examples and are not intended to limit the scope of the invention. These novel embodiments can be implemented in various other forms, and various omissions, substitutions, and modifications can be made without departing from the spirit of the invention. These embodiments and their modifications are included in the scope and spirit of the invention, and are included in the scope of the invention and its equivalents described in the claims.

Claims

1. This is a glycation-improving supplement that contains pressurized enzymatic hydrolysates obtained by heating oysters at 60°C or higher and then subjecting them to an enzymatic reaction under pressure.

2. 2. The glycation improving supplement according to claim 1, wherein the enzymatic reaction is a pressurized enzymatic reaction at a pressure of 40 to 200 MPa and a temperature of 30 to 80° C. for 1 to 48 hours.

3. 3. The glycation improving supplement according to claim 2, wherein the enzymatic reaction is a pressurized enzymatic reaction at a pressure of 50 to 150 MPa and a temperature of 40 to 70° C. for 3 to 24 hours.

4. The glycation improving supplement according to claim 1, wherein the enzyme is a protease.

5. The glycation improving supplement according to claim 4, wherein the enzyme is thermolysin.

6. A step of heat treating the oysters at 60°C or higher; A method for producing a glycation improving supplement includes a step of subjecting the obtained heat-treated product to an enzymatic reaction under pressure.

7. Oysters are heat-treated at 60°C or higher, and then subjected to an enzymatic reaction under pressure to produce a pressurized enzymatic hydrolysate, which is then used as a supplement for improving glycation.