Virus inactivating agent composition

Abstract

To provide a virus inactivating agent composition using natural extracts.SOLUTION: A virus inactivating agent composition contains one or more natural extracts selected from a tian cha extract, an Uncaria gambir extract, an Uncaria tomentosa extract, a Sanguisorba officinalis extract, a Phellodendron amurense extract, a Hypericum erectum extract, a Coptis japonica extract, a Syzygium aromaticum extract, a Scutellaria baicalensis Georgi extract, a Crataegus cuneata extract, a Filipendula ulmaria flower extract, a Paeonia albiflora Pallas extract, a Betula platyphylla extract, a Hedera helix extract, an Achillea millefolium extract, a Thymusserpyllum extract, an Angelica acutiloba extract, a Hamamelis virginiana extract, a Rosa centifolia extract, an Eriobotrya japonica extract, an Aesculus hippocastanum extract, and a Rosmarinus officinalis extract, and a Melissa officinalis extract.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a virus inactivating composition using a natural extract. [Background technology]

[0002] Norovirus, which has become a problem in recent years, is the cause of many food poisoning incidents every year, but it is a virus that is difficult to take food hygiene measures against because it has low drug sensitivity and is difficult to inactivate. Norovirus is a non-enveloped virus with a protein shell called a capsid, so the inactivation effect of alcohol or cationic surfactants is insufficient, and treatment with sodium hypochlorite is recommended. However, when taking sanitation measures in an environment where food is handled, it is desirable for the agent to be safe even if it comes into contact with food or cooking utensils, and it is also desirable for the agent to be highly safe for workers who handle the agent. Therefore, virus inactivating agents and disinfectants using foods or food additives that are expected to have a virus inactivating effect are being considered.

[0003] For example, Patent Document 1 discloses an anti-norovirus agent containing, as an active ingredient, an extract of a plant of the genus Diospyros that contains tannins. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Patent No. 5092145 Summary of the Invention [Problem to be solved by the invention]

[0005] However, this is not sufficient to inactivate non-enveloped viruses, and further virus inactivating agents that use foods or food additives have been required for the safe handling of foods and the like.

[0006] An object of the present invention is to provide a virus inactivating agent that inactivates viruses, particularly non-enveloped viruses. [Means for solving the problem]

[0007] As a result of intensive research, the inventor has discovered that sweet tea, gambir tree ( Uncaria gambir ), Cat's Claw ( Uncaria tomentosa ), Waremokou ( S. officinalis ), Phellodendron bark ( Phellodendron amurense ), Hypericum ( Hypericum erectum ), Oren ( Japanese Coptis ), Choji ( Syzygium aromaticum ), Goldenshrimp ( Scutellaria Baikalensis Georgi ), Hawthorn ( Hawthorn cuneata ), European summer snowflake ( Filipendula ulmaria ), Peony ( Paeonia albiflora Pallas ), Birch ( Birch tree ), English ivy ( Ivy spiral ), Yarrow ( Yarrow ),time( Thymus serpyllum ), Touki ( Angelica acutifolia ), Hamamelis ( Witch hazel ), rose ( Rose centifolia ), loquat ( Eriobotrya japonica ), Horse Chestnut ( Horse chestnut tree ), Rosemary ( Rosemary officinalis ), Melissa ( Melissa officinalis The inventors have found that an extract from the plant has a virus-inactivating effect, which led to the completion of the present invention.

[0008] That is, in order to achieve the above object, the present invention relates to the following [1] to [7]. [1] A virus inactivating composition comprising one or more natural extracts selected from sweet tea extract, gambir tree extract, cat's claw extract, burnet extract, phellodendron bark extract, Hypericum extract, coptis japonica extract, clove extract, scarlet laurel extract, hawthorn extract, meadowsweet flower extract, peony extract, white birch extract, English ivy extract, yarrow extract, thyme extract, angelica extract, hamamelis extract, rose extract, loquat extract, horse chestnut extract, rosemary extract, and melissa extract. [2] The virus inactivating composition according to [1], characterized in that the natural extract contains one or more selected from sweet tea extract, gambir tree extract, Sanguisorba officinalis extract, Phellodendron Bark extract, Hypericum perforatum extract, Coptis japonica extract, and clove extract. [3] The virus inactivating composition according to [1] or [2], further comprising one or more water-soluble solvents selected from organic solvents and water. [4] A virus inactivating composition according to any one of [1] to [3], comprising the natural extract in an amount of 0.0001 to 10% by mass. [5] The virus inactivating composition according to any one of [1] to [4], wherein the water-soluble solvent is one or more selected from the group consisting of alcohol, glycol, glycol ether, and water. [6] A virus inactivating composition according to any one of [1] to [5], which inactivates non-enveloped viruses. [7] A hygiene product comprising the virus inactivating composition according to any one of [1] to [6] above. Effect of the Invention

[0009] According to the present invention, by containing the specific natural extract described in [1] above as an active ingredient, a virus inactivating agent that is highly safe for the human body can be provided, and by containing the specific natural extract described in [2] above as an active ingredient, it can exhibit a high virus inactivating effect even in the presence of organic soil. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] Next, the best mode for carrying out the present invention will be described in detail.

[0011] First, the natural extracts used in the present invention include sweet tea extract, gambir extract, cat's claw extract, burnet extract, Phellodendron bark extract, Hypericum extract, Coptis japonica extract, clove extract, Scutellaria root extract, hawthorn extract, meadowsweet extract, peony extract, white birch extract, English ivy extract, yarrow extract, thyme extract, witch hazel extract, rose extract, loquat extract, horse chestnut extract, rosemary extract, and melissa extract.

[0012] The sweet tea used as the raw material for the above sweet tea extract includes sweet teas from the Rosaceae, Saxifragaceae, Rubiaceae, Fagaceae, etc., but in particular, the sweet tea from Rubus tenuifolia ( Rubus suavissimus S.Lee ) is preferred. The extract includes an extract obtained as an extraction raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or any of these roughly purified or purified products. The parts of sweet tea used as the extraction raw material include leaves, flowers, branches, fruits, bark, roots, etc., and it is particularly preferred to use leaves or branches, or a mixture thereof.

[0013] The components of sweet tea extract include sweet tea polyphenols, which contain ellagitannins. Ellagitannins are classified into GOD type, DOG type, GOG type, etc. based on the bonding pattern, and the sweet tea polyphenol components are GOD type ellagitannins. Components other than polyphenols include rubusoside, etc.

[0014] The above Gambino extract is made by extracting the leaves and young branches of Gambino, which belongs to the genus Uncaria of the Rubiaceae family, with water, ethanol, 1,3-butylene glycol, or other alcohol, filtering, removing the solvent, and then drying, or, if the extraction solvent is highly safe, obtaining the extract without drying. The components of the Gambino extract include catechin, tannin, flavones, alkaloids, etc. Commercially available products include Acacia extracts sold by Koei Kogyo Co., Ltd. and Maruzen Pharmaceutical Co., Ltd.

[0015] The above cat's claw extract is made by extracting the root, bark, leaves, etc. of cat's claw belonging to the genus Uncaria of the Rubiaceae family with water, ethanol, 1,3-butylene glycol, or other alcohol, filtering, removing the solvent, and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. The components of the cat's claw extract include pentacyclic oxindole alkaloids, quinovic acid glucoside, triterpene, proanthocyanidin, etc. Examples of commercially available products include Bizen Kasei Co., Ltd.'s Bisen Cat's Claw Extract Powder.

[0016] The above-mentioned Scutellaria extract is made by extracting the roots or rhizomes of Scutellaria belonging to the genus Scutellaria of the Rosaceae family with water, ethanol, 1,3-butylene glycol, or other alcohol, filtering, removing the solvent, and then drying or, if the extraction solvent is highly safe, obtaining the extract without drying. The components of the Scutellaria extract include tannins and triterpenoid saponins. Commercially available products include Scutellaria extract from Koei Kogyo Co., Ltd., and Jiu extract and Jiu extract powder from Maruzen Pharmaceutical Co., Ltd.

[0017] The above Phellodendron bark extract is prepared by extracting the leaves, stems, flowers, roots, bark, fruit, peel, pulp, or mixtures thereof of Phellodendron bark belonging to the genus Phellodendron of the Rutaceae family with water or alcohol such as ethanol or 1,3-butylene glycol, filtering, removing the solvent, or directly drying the extract if the extraction solvent is highly safe. The bark is preferred as the part of the extraction raw material. Components of Phellodendron bark extract include alkaloids such as berberine, palmatine, and magnophylline, and flavonoids. Commercially available products include Phellodendron bark liquid E from Ichimaru Pharcos Co., Ltd. and Phellodendron bark extract BG- from Maruzen Pharmaceutical Co., Ltd. Examples include J.

[0018] The above-mentioned Hypericum extract is prepared by extracting flowers, leaves, stems, fruits or mixtures thereof of Hypericum perforatum belonging to the genus Hypericum in the family Hypericaceae with water or alcohol such as ethanol or 1,3-butylene glycol, filtering, removing the solvent and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. Components of the Hypericum extract include tannins, anthraquinones such as hypericin and pseudohypericin, essential oils wedelactone, demethylwedelactone, otogirin, otogiron, palmatic acid, oleic acid, linoleic acid, etc. Commercially available products include Ichimaru Pharcos Co., Ltd.'s Examples include Falcolex Hypericum E.

[0019] The Coptis japonica extract is prepared by extracting the rhizomes of Coptis japonica, which belongs to the Ranunculaceae family, with water and alcohol such as ethanol or 1,3-butylene glycol, filtering, removing the solvent, and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. The components of the Coptis japonica extract include alkaloids such as berberine, palmatine, coptisine, jatrorrhizine, aurenin, and magnophylline. Commercially available products include Falcorex Coptis Rhizome E from Ichimaru Pharcos Co., Ltd. and Coptis Rhizome Extract BG30 from Maruzen Pharmaceutical Co., Ltd.

[0020] The above clove extract is prepared by extracting the fruits, flowers, buds, leaves, stems, bark, etc. of the clove tree belonging to the genus Myrtaceae with water or alcohol such as ethanol or 1,3-butylene glycol, filtering, removing the solvent, and then drying, or, if the extraction solvent is highly safe, obtaining the extract without drying. The components of the clove extract include eugenol, acetyleugenol, β-caryophyllene, vanillin, crategoric acid, bicornin, gallotannic acid, methyl salicylate, eugenin, kaempferol, rhamnetin, eugenitin, oleanolic acid, stigmasterol, campesterol, etc. Commercially available products include Clove Extract ET-50-D from Koei Kogyo Co., Ltd. and Falcorex Clove from Ichimaru Pharcos Co., Ltd.

[0021] The above Scutellaria extract is made by extracting the roots of Scutellaria, which belongs to the genus Scutellaria, family Lamiaceae, with water, ethanol, 1,3-butylene glycol, or other alcohol, filtering, removing the solvent, and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. Components of Scutellaria extract include flavonoids, baicalin, baicalein, wogonin, etc. Commercially available products include Scutellaria extract powder from Ichimaru Pharcos Co., Ltd. and Scutellaria extract from Maruzen Pharmaceutical Co., Ltd.

[0022] The above-mentioned hawthorn extract is made by extracting the fruits of the cratae of the genus Crataegus of the Rosaceae family with water, ethanol, 1,3-butylene glycol, or other alcohols, filtering, removing the solvent, and then drying, or, if the extraction solvent is highly safe, obtaining the extract without drying. The components of the hawthorn extract include flavonoids (quercetin), phenols (chlorogenic acid), triterpenoids (ursolic acid), etc. Examples of commercially available products include Falcolex Hawthorn B from Ichimaru Pharcos Co., Ltd. and Hawthorn Extract BG-J from Maruzen Pharmaceutical Co., Ltd.

[0023] The above-mentioned meadowsweet flower extract is made by extracting the meadowsweet flower of the genus Spiraea in the family Rosaceae with water, ethanol, 1,3-butylene glycol or other alcohol, filtering, removing the solvent, and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. Components of the meadowsweet flower extract include salicin, salicylic acid glycosides, tannins, flavonoids, etc. Commercially available products include Falcolex Spiraea B by Ichimaru Pharcos Co., Ltd.

[0024] The above-mentioned peony extract is made by extracting the roots of the peony genus of the Paeoniaceae family with water or alcohol such as ethanol or 1,3-butylene glycol, filtering, removing the solvent, and then drying or, if the extraction solvent is highly safe, obtaining the extract without drying. The components of the peony extract include monoterpene glycosides (paeoniflorin, albiflorin), tannins (garotanin), phenols (beonol), benzoic acid, etc. Commercially available products include Peony Liquid from Ichimaru Pharcos Co., Ltd. and Peony Extract Liquid-J from Maruzen Pharmaceutical Co., Ltd.

[0025] The above-mentioned birch extract is made by extracting the bark of birch of the Betulaceae family with water or alcohol such as ethanol or 1,3-butylene glycol, filtering, removing the solvent, and then drying or, if the extraction solvent is highly safe, obtaining the extract without drying. The components of the birch extract include flavonoids, saponin, tannin, terpenoids (betulin, betulinic acid), etc. Commercially available products include Birch Extract from Ichimaru Pharcos Co., Ltd. and Birch Extract from Maruzen Pharmaceutical Co., Ltd.

[0026] The above-mentioned ivy extract is made by extracting the stems and leaves of ivy of the genus Ivy of the Araliaceae family with water, ethanol, 1,3-butylene glycol, or other alcohol, filtering, removing the solvent, and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. The components of the ivy extract include saponins (hederin), flavonoids (rutin), tannins, etc. Commercially available products include Falcolex Ivy B from Ichimaru Pharcos Co., Ltd. and Ivy Extract BG-J from Maruzen Pharmaceutical Co., Ltd.

[0027] The above-mentioned Achillea millefolium extract is prepared by extracting the whole plant of Achillea millefolium belonging to the Asteraceae family with water, ethanol, 1,3-butylene glycol or other alcohol, filtering, removing the solvent, and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. The components of Achillea millefolium extract include alkaloids, sesquiterpenes, essential oils, etc. Commercially available products include Falcolex Achillea millefolium B from Ichimaru Pharcos Co., Ltd. and Achillea millefolium extract BG-J from Maruzen Pharmaceutical Co., Ltd.

[0028] The above thyme extract is prepared by extracting the whole plant of thyme belonging to the genus Thymus of the Lamiaceae family with water, ethanol, 1,3-butylene glycol, or other alcohol, filtering, removing the solvent, and then drying or, if the extraction solvent is highly safe, obtaining the extract without drying. Components of thyme extract include essential oil (thymol), tannin, saponosides, etc. Commercially available products include thyme extract from Koei Kogyo Co., Ltd.

[0029] The above-mentioned Angelica extract is made by extracting the root of Angelica acutiloba, which belongs to the Apiaceae family, with water, alcohols such as ethanol and 1,3-butylene glycol, or a mixture of these, or by extracting with squalane under heating, filtering, removing the solvent, and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. The components of the Angelica extract include essential oils (ligustilide, safrole, butylidenephthalide), amino acids (alanine, arginine), vitamins, etc. Commercially available products include Angelica acutiloba extract LA from Maruzen Pharmaceutical Co., Ltd. and Angelica acutiloba extract from Koei Kogyo Co., Ltd.

[0030] The above-mentioned Hamamelis extract is made by extracting Hamamelis leaves of Hamamelis genus of Hamamelidaceae with water, alcohols such as ethanol and 1,3-butylene glycol, or a mixture of these, filtering, removing the solvent, and drying, or if the extraction solvent is highly safe, obtaining the extract without drying. Components of Hamamelis extract include tannins (hamamelistannin), saponin, flavonoids (quercetin), etc. Commercially available products include Falcolex Hamamelis B from Ichimaru Pharcos Co., Ltd. and Hamamelis Extract from Koei Kogyo Co., Ltd.

[0031] The above-mentioned rose extract is made by extracting the flowers of Rosa centifolia, which belongs to the Rosaceae family, with water, alcohols such as ethanol or 1,3-butylene glycol, or a mixture of these, filtering, removing the solvent, and then drying, or, if the extraction solvent is highly safe, obtaining the extract without drying. The components of the rose extract include polyphenols, vitamin C, etc. Commercially available products include Falcolex Rose P from Ichimaru Falcos Co., Ltd. and Rose Extract S from Koei Kogyo Co., Ltd.

[0032] The loquat extract is made by extracting the leaves of loquat (Loquat genus, Rosaceae) with water, alcohols such as ethanol or 1,3-butylene glycol, or a mixture of these, filtering, removing the solvent, and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. The components of loquat extract include sugars (glucose, sucrose, fructose, maltose, starch, dextrin), organic acids (tartaric acid, citric acid, malic acid), amygdalin, tannins, etc. Commercially available products include Loquat Extract-J from Maruzen Pharmaceutical Co., Ltd. and Loquat Extract from Koei Kogyo Co., Ltd.

[0033] The above horse chestnut extract is made by extracting the seeds of the horse chestnut tree of the Aesculus genus in the Aesculaceae family with water, alcohols such as ethanol or 1,3-butylene glycol, or a mixture of these, filtering, removing the solvent, and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. The components of the horse chestnut extract include triterpene saponin (escin), catechin tannin, etc. Commercially available products include Falcorex Horse Chestnut B from Ichimaru Pharcos Co., Ltd. and Horse Chestnut Extract from Koei Kogyo Co., Ltd.

[0034] The above-mentioned rosemary extract is made by extracting the leaves of Rosemary, which belongs to the Lamiaceae family, with water, alcohols such as ethanol or 1,3-butylene glycol, or a mixture of these, filtering, removing the solvent, and then drying, or, if the extraction solvent is highly safe, obtaining the extract without drying. The components of rosemary extract include diterpene compounds (carnosic acid, carnosol, rosmanol), polyphenols (rosmarinic acid), flavonoids (luteolin), phenolic carboxylic acids, essential oils (cineole, These include borneol, borneol esters, tannins, etc. Commercially available products include Falcolex Rosemary E from Ichimaru Falcos Co., Ltd. and Mannenrou Extract from Koei Kogyo Co., Ltd.

[0035] The above-mentioned Melissa extract is made by extracting leaves of Melissa genus of the Lamiaceae family with water, alcohols such as ethanol and 1,3-butylene glycol, or a mixture of these, filtering, removing the solvent, and then drying, or if the extraction solvent is highly safe, obtaining the extract without drying. Components of Melissa extract include tannins, flavonoids, gallic acid, phenolic acids (rosmarinic acid), essential oils (citral, linalool), etc. Commercially available products include Falcolex Melissa B from Ichimaru Pharcos Co., Ltd. and Melissa Extract-J from Maruzen Pharmaceutical Co., Ltd.

[0036] The virus inactivating composition of the present invention has a high inactivating effect, particularly against non-enveloped viruses that have low drug susceptibility.

[0037] The non-enveloped viruses include the Caliciviridae family ( Caliciviridae ) virus, Picornaviridae ( Picornaviridae ) virus, Parvoviridae ( Parvoviridae ) virus, Papillomaviridae ( Papillomaviruses ) virus, Polyomaviridae ( Polyomaviruses ) virus, Adenoviridae ( Adenoviridae ) viruses. The Caliciviridae family includes the Vesivirus genus ( Vesivirus ) virus, Norovirus genus ( Norovirus ) virus, Sapovirus genus ( Sapov going ) Viruses, etc.

[0038] Among the above natural extracts, it is particularly preferable to contain one or more of sweet tea extract, gambir tree extract, burnet extract, Phellodendron bark extract, Hypericum perforatum extract, Coptis japonica extract, and clove extract, in order to maintain a sufficient inactivation effect against non-enveloped viruses even in the presence of organic soil, i.e., under conditions of contact with or mixture with organic soil.

[0039] The content of the natural extract contained in the virus inactivating composition of the present invention is preferably 0.0001 to 10% by mass from the viewpoint of virus inactivation performance, more preferably 0.001 to 8% by mass, and even more preferably 0.01 to 5% by mass. The higher the content of the natural extract, the higher the virus inactivation effect, but if the content is too high, it is disadvantageous in terms of the overall balance of the composition, and may be economically disadvantageous due to reduced storage stability or approaching the upper limit of the virus inactivation effect.

[0040] When the virus inactivating composition of the present invention is used in a liquid form, the natural extract can be dissolved in water or an organic solvent and then used, although there is no particular limitation.

[0041] The water may be tap water, soft water, ion-exchanged water, pure water, purified water, etc., with tap water, soft water, and ion-exchanged water being preferred.

[0042] Examples of the organic solvent include lower alcohols having 1 to 5 carbon atoms, such as ethanol and isopropanol; lower aliphatic ketones, such as acetone and methyl ethyl ketone; polyhydric alcohols having 2 to 5 carbon atoms, such as 1,3-butylene glycol, propylene glycol, triethylene glycol, hexylene glycol, and glycerin; ethylene glycol ethers, such as ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monopropyl ether, and ethylene glycol monobutyl ether; diethylene glycol ethers, such as diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monopropyl ether, and diethylene glycol monobutyl ether; glycol ethers, such as propylene glycol monomethyl ether and dipropylene glycol monomethyl ether, and mixtures of the organic solvents and water can also be used. Preferred organic solvents are ethanol, propylene glycol, 1,3-butylene glycol, and glycerin.

[0043] The virus inactivating composition of the present invention can be provided in various forms such as a liquid, a gel, a jelly, or a powder.

[0044] The virus inactivating composition of the present invention may be used as it is without dilution, but may be used after dilution with water for ease of handling and economic reasons. In particular, when used in a powder form, it is preferable to dilute with water from the viewpoint of virus inactivation effect. When diluted, it can be used after dilution to 0.0001 to 10% by mass. More preferably, it is 0.01 to 5% by mass.

[0045] Among the above organic solvents, it is preferable to include alcohol in terms of the bactericidal effect. Examples of alcohol include ethanol, isopropanol, and methanol, and preferably ethanol and isopropanol. Those selected from these can be used alone or in combination of two or more. In particular, ethanol can be preferably used in terms of safety and toxicity.

[0046] The content of alcohol contained in the virus inactivating composition of the present invention is preferably 35 to 85% by mass from the viewpoint of bactericidal effect. More preferably, it is 40 to 75% by mass, and even more preferably, it is 50 to 70% by mass. From the viewpoint of bactericidal effect, the higher the alcohol content, the higher the effect. However, if the alcohol content is 60% or more by mass, it is treated as a dangerous material under the Fire Service Act, so that if the application involves restrictions on handling, it is preferable that the alcohol content is less than 60% by mass. In addition, a high alcohol content may be disadvantageous in terms of the solubility of the natural extract.

[0047] When the alcohol content in the composition of the present invention is 35 to 85% by mass, the composition has a bactericidal effect in addition to the above-mentioned virus inactivation effect. Examples of the bacteria include food poisoning bacteria, such as Staphylococcus aureus, pathogenic Escherichia coli, Salmonella, Campylobacter, Vibrio parahaemolyticus, Clostridium botulinum, and Clostridium perfringens.

[0048] The virus inactivating composition of the present invention may further contain a surfactant as an optional component. Examples of the surfactant include anionic surfactants, nonionic surfactants, cationic surfactants, and amphoteric surfactants. These surfactants may be used alone or in combination of two or more kinds.

[0049] Examples of the anionic surfactant include carboxylic acids such as aliphatic monocarboxylates, polyoxyethylene alkyl ether carboxylates, N-acylsarcosine salts, and N-acylglutamates, sulfonic acids such as dialkylsulfosuccinates, alkanesulfonates, α-olefinsulfonates, alkylbenzenesulfonates, alkylnaphthalenesulfonates, and N-methyl-N-acyltaurate salts, sulfates such as alkyl sulfates, polyoxyethylene alkyl ether sulfates, and fat sulfates, and phosphates such as alkyl phosphates, polyoxyethylene alkyl ether phosphates, and polyoxyethylene alkyl phenyl ether phosphates. Among these, sulfonic acids are preferred, and alkanesulfonates, α-olefinsulfonates, and alkylbenzenesulfonates are particularly preferred. These may be used alone or in combination of two or more.

[0050] Examples of the nonionic surfactant include esters such as glycerin fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene alkyl ether, polyoxyethylene alkenyl ether, polyoxyethylene alkylphenyl ether, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene polyoxypropylene alkenyl ether, polyoxyethylene polyoxybutylene alkyl ether, alkylamine ethylene oxide adduct, alkylamine ethylene oxide adduct propylene oxide adduct, ethers such as polyoxyethylene polyoxypropylene glycol, fatty acid polyethylene glycol, fatty acid polyoxyethylene sorbitan ester ethers, alkanolamides such as fatty acid alkanolamide, alkyl glycoside, etc., but are not limited thereto.Among them, esters are preferred, and glycerin fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester are particularly preferred.In addition, these may be used alone or in combination of two or more kinds.

[0051] The cationic surfactant includes alkylamine salt and quaternary ammonium salt.The alkylamine salt includes monoalkylamine salt, dialkylamine salt, and trialkylamine salt, and the quaternary ammonium salt includes benzalkonium chloride, benzethonium chloride, didecyldimethylammonium chloride, tetramethylammonium chloride, tetrabutylammonium chloride, methylbenzethonium chloride, distearyldimethylammonium chloride, cetylpyridinium chloride, alkyltrimethylammonium chloride, octyltrimethylammonium chloride, decyltrimethylammonium chloride, dodecyldimethylbenzylammonium chloride, dodecyltrimethylammonium chloride, tetradecyltrimethylammonium chloride, hexadecyltrimethylammonium chloride, cetyltrimethylammonium chloride, stearyltrimethylammonium chloride, benzyltrimethylammonium chloride, benzyltriethylammonium chloride, dialkyldimethylammonium chloride, tetramethylammonium hydroxide, benzalkonium bromide, cetrimonium bromide, domiphen bromide, alkyltrimethylammonium bromide, and hexadecyltrimethylammonium bromide, but is not limited thereto. These may be used alone or in combination of two or more.

[0052] Examples of the amphoteric surfactant include, but are not limited to, alkyl betaines, carboxy betaines such as fatty acid amidopropyl betaines, 2-alkyl imidazoline derivatives such as 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaines, glycine surfactants such as alkyl diethylene triamino acetic acid and dialkyl diethylene triamino acetic acid, alkyl amine oxides, etc. These may be used alone or in combination of two or more kinds.

[0053] If necessary, a pH adjuster can be added to the virus inactivating composition of the present invention. The pH is adjusted using an alkaline substance and an acidic substance.

[0054] Examples of alkaline substances used to adjust the pH include alkali hydroxides such as sodium hydroxide and potassium hydroxide, carbonates such as sodium carbonate and potassium carbonate, silicates such as sodium silicate and potassium silicate, amines such as monoethanolamine and triethanolamine, and ammonia. Examples of acidic substances used to adjust the pH include inorganic acids such as hydrochloric acid, sulfuric acid, and phosphoric acid, and organic acids such as citric acid, acetic acid, lactic acid, malic acid, gluconic acid, succinic acid, and tartaric acid.

[0055] Furthermore, the virus inactivating composition of the present invention may contain other additives, such as skin irritation mitigating agents, solvents other than those mentioned above, thickeners, chelating agents, deodorants, dyes, fragrances, preservatives, metal corrosion inhibitors, bactericides such as hop extracts and polylysine, other surfactants, and denaturants such as flavors H-No. 11 and H-13, as necessary, within the range that does not impair the effects of the present invention. Examples of skin irritation mitigating agents include those with moisturizing effects, such as aloe extract, loofah extract, perilla extract, and sage extract.

[0056] The virus inactivating composition of the present invention may be used as is without dilution, but may be appropriately diluted, for example, 2-fold to 200-fold, 3-fold to 150-fold, 5-fold to 100-fold, etc., as long as the virus can be inactivated. Dilution can be performed using water.

[0057] Sterilization and disinfection of a target surface using the virus inactivating composition of the present invention can be carried out by the following method.

[0058] (1) The virus inactivating composition of the present invention can be impregnated into a nonwoven fabric to produce a sanitary material, which can be wiped onto a target surface for disinfection. Places where disinfection can be performed include medical facilities, nursing care facilities, food factories, coffee shops, restaurants, hotels, pubs, schools (school lunches), central kitchens, supermarket backyards, etc., and examples of target surfaces include kitchen counters, refrigerators, storage units, as well as tables, desks, chairs, handrails on stairs and corridors, doorknobs at entrances and exits, toilets and toilet seats, water faucets, floors and walls, and other hard surfaces. The sanitary material of the present invention can also be used as hand towels, wet tissues, etc.

[0059] Examples of raw materials for the nonwoven fabric constituting the sanitary material of the present invention include cellulosic materials such as cotton, protein-based materials such as wool or silk, and chemically polymerized materials such as rayon, polyester, and acrylic. Of these, cellulosic materials and chemically polymerized materials are preferred.

[0060] (2) The virus inactivating composition of the present invention can be used by any suitable method other than those described above. For example, it is sprayed onto the target surface by a sprayer or the like, and after a predetermined time, it is left to stand for about 30 seconds to 5 minutes, and then naturally dried as is, or it is appropriately rinsed with water and then dried. Specifically, it is dispensed at about 6 to 12 ml / m2 using a dedicated dispenser containing the virus inactivating composition of the present invention. 2 Disinfection can be achieved by spraying the target surface at a rate of

[0061] The virus inactivating composition of the present invention can be provided by filling it into a plastic container, a container with a pump, a pouch, a tube, etc. Also, it can be provided in an amount equivalent to that for one-time use, individually packaged to make it portable. EXAMPLES

[0062] Next, examples will be described together with comparative examples, but the present invention is not limited to these.

[0063] First, in order to adjust the composition, each product was prepared as follows. The pure content of the natural extract in each product is as follows. Ingredients other than the natural extract are shown in Tables 1 to 4. Products containing 100% natural extract were prepared into 1% by mass aqueous solutions and subjected to testing. The values ​​in the tables indicate the pure content of each ingredient. Unless otherwise specified, "%" refers to mass basis. Furthermore, tap water was used as water unless otherwise specified.

[0064] <Natural extracts> Example 1: Gambir extract: Product name: "Acacia oleracea extract" (Acacia extract 1%, manufactured by Koei Kogyo Co., Ltd.) Example 2: Phellodendron bark extract: Product name "Phellodendron bark liquid E" (0.97% Phellodendron Bark Extract, Ichimaru Pharcos) Example 3: Coptis extract: Trade name "Falcorex Coptis E" (Coptis extract 0.6%, Ichimaru Pharcos) Example 4: Hypericum extract: Trade name "Falcorex Hypericum E" (Hypericum extract 0.86%, Ichimaru Pharcos) Example 5: Sanguisorba officinalis extract: Product name "Sanguisorba officinalis extract" (1.6% Sanguinea Pig Extract, manufactured by Koei Kogyo Co., Ltd.) Example 6: Sweet Tea Extract: Product Name "Tencha Extract M Powder" (100% sweet tea extract, manufactured by Maruzen Pharmaceuticals) Example 7: Clove extract: Trade name "Falcorex Clove" (Clove extract 3.16%, Ichimaru Pharcos) Example 8: Hamamelis extract: Trade name "Falcolex Hamamelis" (Witch hazel leaf extract 0.14%, Ichimaru Falcos) Example 9: Rose extract: Product name "Falcorex Rose P" (Rose extract 1.0%, Ichimaru Pharcos) Example 10: Horse Chestnut Extract: Product Name "Falcorex Horse Chestnut B" (1.0% Horse Chestnut Extract, Ichimaru Pharcos) Example 11: Rosemary extract: Product name "Rosemary extract" (Rosemary leaf extract 1.0%, manufactured by Koei Kogyo Co., Ltd.) Example 12: Cat's Claw Extract: Product Name "Bizen Cat's Claw Extract Powder" (100% cat's claw extract, manufactured by Bizen Kasei Co., Ltd.) Example 13: Scutellaria extract: Product name "Scutellaria extract powder" (100% Scutellaria root extract, manufactured by Ichimaru Falcos) Example 14: Hawthorn extract: Trade name "Falcorex Hawthorn B" (Hawthorn extract 0.34%, Ichimaru Pharcos) Example 15: Meadowsweet Flower Extract: Product name: Falco Rex Spirea B (Meadowsweet flower extract 0.2%, Ichimaru Pharcos) Example 16: Peony extract: Product name "Peony Liquid" (Peony root extract 2.23%, Ichimaru Falcos) Example 17: Birch extract: Product name "Birch Extract" (Birch bark extract 0.13%, Ichimaru Falcos) Example 18: Thyme extract: Product name "Thyme extract" (Thyme extract 0.5%, manufactured by Koei Kogyo Co., Ltd.) Example 19: Angelica acutiloba extract: Product name "Angelica acutiloba extract" (Angelica Root Extract 2.0%, manufactured by Koei Kogyo Co., Ltd.) Example 20: Loquat extract: Product name "Loquat extract" (Loquat leaf extract 0.5%, manufactured by Koei Kogyo Co., Ltd.) Example 21: Melissa extract: Product name "Falcorex Melissa B" (Melissa leaf extract 0.8%, Ichimaru Falcos) Comparative Example 1: Houttuynia cordata extract: Product name "Houttuynia cordata extract ET-50" (0.8% Houttuynia cordata extract, manufactured by Koei Kogyo Co., Ltd.) Comparative Example 2: Ginkgo extract: Product name "Ginkgo extract BG-50" (Ginkgo leaf extract 0.6%, manufactured by Koei Kogyo Co., Ltd.)

[0065] [Examples 1 to 35, Comparative Examples 1 to 3] Compositions having the compositions shown in Tables 1 to 7 below (the units of values ​​in each table are "mass %") were prepared and evaluated for their virus inactivation effects. The test methods and evaluation criteria for each item are as follows.

[0066] The compositions of Examples 1 to 35 and Comparative Examples 1 to 3 shown in Tables 1 to 7 were tested using feline calicivirus ( feline calicivirus The FCV inactivation effect of each was evaluated.

[0067] [FCV inactivation effect] Testing Method Using FCV, a surrogate virus for norovirus, the virus inactivation agent compositions of the examples and comparative examples were subjected to FCV inactivation tests. The tests were performed according to known methods (Food Hygiene Inspection Guidelines, Microorganism Edition (2015), "Virus Inactivation Test"; "Report on the Inactivation Conditions for Norovirus in 2015," Food Hygiene Management Department, National Institute of Health Sciences, etc.).

[0068] (a) No meat extract added (no stains) That is, the test virus solution (FCV F9 strain) prepared by the above method and the virus inactivating agent composition of the Example and Comparative Example were mixed in a ratio of 1:9 to prepare a reaction solution, which was left at room temperature for 30 seconds and diluted 7-fold with MEM medium supplemented with 2% fetal bovine serum (FBS) to terminate the reaction. The diluted reaction solution was further serially diluted 7-fold with MEM medium, and the diluted solution was inoculated into feline kidney-derived cells (CRFK cells) to obtain a 50% infection endpoint (TCID 50 The amount of viable virus was quantified in units of 0.01% / mL. (b) With meat extract added (dirty) The above test virus solution (FCV F9 strain) was mixed with equal amounts of MEM medium supplemented with 10% meat extract (Nacalai Tesque) to prepare a virus solution containing 5% meat extract, and the amount of viable virus in this virus solution was quantified in the same manner as in the case without the addition of meat extract.

[0069] The diluted reaction solution after the reaction was stopped was diluted 7-fold, and the actual measured value (log 7 ) to log 10 The inactivation of FCV was evaluated based on the converted value, i.e., the difference between the amount of viable virus and the amount of virus contained in the inoculated dilution [infectivity titer (log 10 The results were evaluated based on the following criteria, according to the difference between the measured values ​​(converted values) and the measured values ​​(calculated values). Evaluation criteria ◎ (sufficient effect): 2 logs 10 A decrease of more than . ○(Effective): 1 log 10 2 logs above 10 A decrease of less than. ×(no effect):1log 10 A decrease of less than.

[0070] [Table 1]

[0071] [Table 2]

[0072] [Table 3]

[0073] [Table 4]

[0074] From the evaluation results in Tables 1 to 4 above, it can be seen that all of Examples 1 to 21 have an FCV inactivation effect. In particular, the sweet tea extract, gambir extract, burnet extract, Phellodendron bark extract, Hypericum perforatum extract, Coptis extract, and clove extract showed an FCV inactivation effect even under soiled conditions. In cases where hygiene measures are required, the target surface is often contaminated with soiling containing organic matter, and the contaminated surface is usually cleaned and then a disinfectant is applied. Therefore, a virus inactivating agent composition containing these natural extracts is more suitable in terms of workability, which can inactivate viruses even in the presence of organic soiling, and in terms of protecting workers from viruses and workability. In contrast, in Comparative Examples 1 and 2, no FCV inactivation effect was observed even under unsoiled conditions.

[0075] Next, among the above natural extracts, those with particularly high FCV inactivation effects were selected and added to an ethanol aqueous solution at low concentrations to prepare an FCV inactivation effect test. An ethanol aqueous solution not containing the natural extract (Comparative Example 3) was used as a control and evaluated as follows. Evaluation criteria ◎: 2 log lower than Comparative Example 3 10 A decrease of more than . ○: 1 log lower than Comparative Example 3 10 2 logs above 10 A decrease of less than.

[0076] [Table 5]

[0077] [Table 6]

[0078] [Table 7]

[0079] From the evaluation results in Tables 5 to 7 above, it was found that all of Examples 22 to 35, which contained low concentrations of natural extracts, had a greater FCV inactivation effect than an aqueous ethanol solution to which no natural extract was added. Note that, since the compositions of Examples 22 to 35 contained 50% by mass or more of ethanol or isopropanol, it was confirmed that they had a bactericidal effect against Escherichia coli and Staphylococcus aureus.

[0080] The above results demonstrate that the virus inactivating agent of the present invention is highly safe for the human body, and furthermore, by containing specific natural extracts as active ingredients, it is able to exert a high virus inactivating effect even in the presence of organic soil.

Claims

1. Sweet tea extract, gambir tree extract, cat's claw extract, burnet extract, scutellaria extract Tapir extract, Hypericum extract, Coptis extract, Clove extract, Scutellaria extract , Hawthorn extract, Meadowsweet flower extract, Peony extract, Birch extract , English ivy extract, English yarrow extract, thyme extract, Japanese angelica extract, Hamamelis extract, rose extract, loquat extract, horse chestnut extract, rosemary extract, A virus-inhibiting agent comprising one or more natural extracts selected from the group consisting of lyssa extracts, Activator composition.

2. The natural extract is selected from the group consisting of sweet tea extract, gambir tree extract, burnet extract, and phellodendron bark extract. extract, Hypericum extract, Coptis japonica extract, and Clove extract. The virus inactivating composition according to claim 1, characterized in that it contains

3. The composition further contains at least one water-soluble solvent selected from organic solvents and water. The virus inactivating composition according to claim 1 or 2.

4. The composition according to any one of claims 1 to 3, characterized in that the natural extract is contained in an amount of 0.0001 to 10% by mass. A virus inactivating composition.

5. The water-soluble solvent is selected from alcohols, glycols, glycol ethers, and water.

5. The virus inactivating composition according to claim 1, wherein the virus inactivating composition comprises one or more of the following:

6. The method according to any one of claims 1 to 5, characterized in that it inactivates non-enveloped viruses. A virus inactivating composition.

7. A hygiene product comprising the virus inactivating composition according to any one of claims 1 to 6. Materials.

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