Method for inhibiting bolting in intergeneric hybrids of brassicaceae family, method for producing seedlings of intergeneric hybrids of brassicaceae family, and method for producing vegetables of intergeneric hybrids of brassicaceae family

Abstract

To suppress bolting in intergeneric hybrids of the Brassicaceae family.SOLUTION: The method for inhibiting bolting in intergeneric hybrids of the Brassicaceae family according to the present invention comprises at least selection. In the selection, seedlings of intergeneric hybrids of the Brassicaceae family are selected using the height of the growing point as an index. The method for producing seedlings of intergeneric hybrids of the Brassicaceae family according to the present invention comprises at least selection. In the selection, seedlings of intergeneric hybrids of the Brassicaceae family are selected using the height of the growing point as an index. The method for producing vegetables of intergeneric hybrids of the Brassicaceae family according to the present invention comprises at least selection, planting, and harvesting. In the selection, seedlings of intergeneric hybrids of the Brassicaceae family are selected using the height of the growing point as an index. In the planting, the seedlings selected by the selection are planted. In the harvesting, at least leaves of intergeneric hybrids of the Brassicaceae family are harvested.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to a method for suppressing bolting of cruciferous intergeneric hybrids, a method for producing seedlings of cruciferous intergeneric hybrids, and a method for producing cruciferous intergeneric hybrid vegetables.

Background Art

[0002] In recent years, what the market demands are vegetables with new values. In developing new vegetables, one of the candidates is cruciferous vegetables. The reason for taking cruciferous vegetables as one of the candidates is that there are many vegetables with food experience. Included in cruciferous vegetables are cruciferous vegetables (for example, Chinese cabbage, turnip, cabbage, broccoli, kale, etc.), radish vegetables (for example, daikon radish, etc.), caper vegetables (for example, caper, etc.), rocket vegetables (for example, arugula, etc.), wasabi vegetables (for example, wasabi, etc.). ), radish vegetables (for example, daikon radish, etc.), caper vegetables (for example, caper, etc.), rocket vegetables (for example, arugula, etc.), wasabi vegetables (for example, wasabi, etc.).

[0003] There are various methods for developing new cruciferous vegetables. For example, they are interspecific hybridization and intergeneric hybridization, etc. Interspecific hybrids of Brassicaceae are widely spread. For example, rapeseed, etc. On the other hand, intergeneric hybrids of Brassicaceae are not widespread. The reason why intergeneric hybrids of Brassicaceae are not widespread is that they show infertility. It is difficult to propagate plants showing infertility. One of the methods to solve this is tissue culture. For example, Patent Document 1 discloses a method for producing seedlings of cruciferous intergeneric hybrids. Patent Document 2 discloses a method for producing seedlings of salad rocket.

Prior Art Documents

Patent Documents

[0004] [Patent Document 1] WO2020 / 196388 [Patent Document 2] Japanese Patent Application Laid-Open No. 06-343360 [Summary of the Invention] [Problems to be Solved by the Invention]

[0005] The problem to be solved by the present invention is to suppress bolting of Brassica interspecific hybrids. Brassica plants may bolt under conditions of temperature and day length. Furthermore, when tissue culture is repeated, there is a tendency for bolting to occur more easily. When bolting occurs, the resource allocation in the plant changes from vegetative growth to reproductive growth. That is, when bolting occurs, the growth of leaves, which are vegetative organs, slows down. In vegetables where the leaves are the harvest target, bolting is one of the factors that reduces the yield. [Means for Solving the Problems]

[0006] Based on the above, what the inventor of the present application has intensively studied and found is that there is a relationship between the height of the growth point of the seedling and the ease of bolting occurrence. That is, by using the height of the growth point of the seedling as an index, it becomes possible to select individuals that are less likely to bolt.

[0007] From this perspective, the present invention is defined as follows.

[0008] The method for suppressing bolting of Brassica interspecific hybrids according to the present invention comprises at least selection. In the selection, seedlings of Brassica interspecific hybrids are selected using the height of the growth point as an index.

[0009] The method for producing seedlings of Brassica interspecific hybrids according to the present invention comprises at least selection. Yes. In the selection, seedlings of intergeneric hybrids of the Brassicaceae family are selected using the height of the growing point as an indicator. .

[0010] The method for producing vegetables of intergeneric hybrids of the Brassicaceae family according to the present invention comprises at least selection , planting, and harvesting. In the selection, seedlings of intergeneric hybrids of the Brassicaceae family are selected using the height of the growing point as an indicator. In the planting, the seedlings selected by the above selection are planted. In the harvesting , at least the leaves of intergeneric hybrids of the Brassicaceae family are harvested.

Advantages of the Invention

[0011] What the present invention enables is to suppress bolting of intergeneric hybrids of the Brassicaceae family. That is , to increase the yield of intergeneric hybrids of the Brassicaceae family.

Brief Description of the Drawings

[0012]

Figure 1

Figure 2

Figure 3

Figure 4

Embodiments for Carrying Out the Invention

[0013] <Intergeneric hybrids of the Brassicaceae family> Intergeneric hybrids of the Brassicaceae family refer to plants belonging to the Brassicaceae family (Brassicacear) and represent plants born from crossing plants belonging to different genera with each other. Examples of plants belonging to the Brassicaceae family include Brassica plants (Chinese cabbage, turnip, Chinese mustard, Komatsuna, Ta Kana, Mizuna, Cabbage, Broccoli, Cauliflower, Brussels Sprouts, Kohlrabi, Kale, etc., Raphanus plants (Radish, etc.), Armoracia plants (Watercress, etc.), Sinapis plants (White Mustard, etc.), Eruca plants (Arugula, etc.), Armoracia rusticana plants (Horseradish, etc.), Wasabi plants (Wasabi, etc.). Preferably, it is an intergeneric hybrid between Brassica plants and Raphanus plants, more preferably, an intergeneric hybrid between Kale and Radish. Most preferably, it is the Ministry of Agriculture, Forestry and Fisheries Variety Registration Application No. 33291 (Name of the applied variety: Santéveil 48) or the Ministry of Agriculture, Forestry and Fisheries Variety Registration Application No. 37030 (Name of the applied variety: SGS 20R01-15). plants (such as), Raphanus plants (such as radish), Armoracia plants (such as watercress), Sinapis plants (such as white mustard), Eruca plants (such as arugula), Armoracia rusticana plants (such as horseradish), Wasabi plants (such as wasabi). Preferably, it is an intergeneric hybrid between Brassica plants and Raphanus plants, more preferably, an intergeneric hybrid between Kale and Radish. Most preferably, it is the Ministry of Agriculture, Forestry and Fisheries Variety Registration Application No. 33291 (Name of the applied variety: Santéveil 48) or the Ministry of Agriculture, Forestry and Fisheries Variety Registration Application No. 37030 (Name of the applied variety: SGS 20R01-15).

[0014] <Method for suppressing bolting of intergeneric hybrid of Brassicaceae according to this embodiment> Figure 1 shows the flow of the method for suppressing bolting of the intergeneric hybrid of Brassicaceae according to this embodiment (hereinafter referred to as "this method"). This method mainly consists of acclimatization (S11) and selection ( S12).

[0015] <Acclimatization (S11)> In this application, acclimatization means transplanting the seedlings produced by tissue culture from under aseptic conditions to the external environment and growing them in the external environment. The purpose of acclimatization is to grow the seedlings produced by tissue culture to a state where they can also grow in the external environment. The first day after acclimatization refers to the day when the seedlings are transplanted from under aseptic conditions to the external environment, and the second day after acclimatization refers to the day after that. The first week after acclimatization refers to the period from the first day to the seventh day after acclimatization. The second week after acclimatization refers to the period from the eighth day to the fourteenth day after acclimatization. The third week after acclimatization refers to the period from the fifteenth day to the twenty-first day after acclimatization. Acclimatization ​​​​​​​​The fourth week after acclimatization refers to the period from the 22nd day to the 28th day after acclimatization, and the fifth week after acclimatization refers to the period from the 29th day to the 35th day after acclimatization. The sixth week after acclimatization refers to the period from the 36th day to the 42nd day after acclimatization. The means of acclimatization is not particularly limited. For example, by obtaining seedlings that have already been acclimatized, acclimatization may be omitted as appropriate.

[0016] <Selection (S12)> In the selection, seedlings of the Brassica intergeneric hybrid are selected. The index for selection is the height of the growing point. The reason for using the height of the growing point as an index is that there is a relationship between the height of the growing point of the seedling and the ease of bolting. That is, seedlings with a low height of the growing point are less likely to bolt. The selection procedure is not particularly limited. For example, it may be carried out by visual inspection and manual work, or by using machinery or the like.

[0017] Preferably, the selection index is, in addition to the height of the growing point, the plant height. The reason for adding the plant height as an index is to reduce the influence of varietal differences and / or cultivation conditions. The height of the growing point may vary due to varietal differences and cultivation conditions. On the other hand, there is a certain correlation between the height of the growing point and the plant height. That is, by using these as indices, it is possible to reduce the influence of varietal differences and / or cultivation conditions.

[0018] <Height of the growing point> In the present application, the height of the growing point refers to the distance from the ground level to the growing point at the top end of the main stem. Figure 2 shows a schematic diagram of a seedling of the Brassica intergeneric hybrid. The ground level refers to the boundary between the base of the Brassica intergeneric hybrid plant and the ground. For example, the height of the growing point of the Brassica intergeneric hybrid shown in Figure 2 is L1. The means for measuring the height of the growing point is not particularly limited. For example, a ruler, ​​​​​​​​​​​​​It may be performed using a measuring tool such as a ruler or calipers, or by using a machine or the like.

[0019] In the present application, "selecting those with a low height of the growing point" means selecting those with a height of the growing point lower than a specific value, excluding those with a height of the growing point higher than a specific value, or selecting those with a height lower than the average value of the height of the growing point in a certain group. The specific numerical value varies depending on the target Brassica intergeneric hybrid and cultivation conditions until selection, and thus is not particularly limited. Preferably, those with a height of the growing point less than the average value of the group produced at the same time are selected. More preferably, those with a height of the growing point less than 1.8 cm are selected, and most preferably, those with a height of the growing point of 0.7 cm or less are selected.

[0020] <Time for measuring the height of the growing point> The time for measuring the height of the growing point may be before the seedlings are planted, and is not particularly limited. Preferably, it is from the 4th week to the 5th week after acclimation. By measuring the height of the growing point at this time, it is possible to more accurately select individuals that are less likely to bolt. From another aspect, preferably, it is a time when the plant height is in the range of 6 cm to 19 cm. By measuring the height of the growing point at this time, it is possible to more accurately select individuals that are less likely to bolt.

[0021] <Plant height> In the present application, the plant height refers to the distance from the ground level to the top of the highest leaf. Figure 2 shows a schematic diagram of a seedling of a Brassica intergeneric hybrid. For example, the plant height of the Brassica intergeneric hybrid seedling shown in Figure 2 is L2. The means for measuring the plant height is not particularly limited. For example, a ruler, calipers, It may be performed using calipers, a ruler, etc., or may be performed using a machine or the like.

[0022] <Relationship between the height of the growing point and plant height> When the selection criteria in this method are the height of the growing point and the plant height, the selected Brassicaceae interspecific hybrids preferably satisfy the following relationship between [A] the height of the growing point (cm) and [B] the plant height (cm). Preferably, it satisfies the formula. Preferably, B / A ≥ 8.5. More preferably, B / A ≥ 10. Even more preferably, B / A ≥ 15.

[0023] <Timing of measuring plant height> The timing of measuring the plant height may be before the seedlings are planted, and is not particularly limited. Preferably it is the same period as the timing of measuring the height of the growing point.

[0024] <Bolting suppression> In the present application, bolting suppression means that bolting does not occur or the occurrence of bolting is delayed in the Brassicaceae interspecific hybrid.

[0025] <Method for producing seedlings of Brassicaceae interspecific hybrid according to this embodiment> Figure 3 shows the flow of the method for producing seedlings of the Brassicaceae interspecific hybrid according to this embodiment (hereinafter referred to as "this seedling production method"). The main components of this seedling production method are acclimation ( S21) and selection (S22). The acclimation (S21) and selection (S 22) in this seedling production method are in accordance with the acclimation (S11) and selection (S12) in this method. The selected ones may be used as seedlings as they are, or clones may be obtained from the selected ones by further tissue culture or the like to be used as seedlings. Here, the content of "Tissue and Cell Culture and Propagation of Vegetables" (edited by the Editorial Committee of the Encyclopedia of the Latest Biotechnology) and WO202 are incorporated herein for reference as appropriate.​ It is the content of 0 / 196388.

[0026] <Method for producing a cruciferous intergeneric hybrid vegetable according to this embodiment> Figure 4 shows the flow of the method for producing a cruciferous intergeneric hybrid vegetable according to this embodiment (hereinafter referred to as " "this vegetable production method"). The production method of this vegetable mainly consists of Acclimatization (S31), selection (S32), planting (S33), cultivation (S34) and harvesting (S35) Acclimatization (S31) and selection (S32) in the production method of this vegetable are in accordance with the acclimatization (S11) and selection (S12) in this method.

[0027] <Planting (S33)> In planting, seedlings of cruciferous intergeneric hybrids are planted. The seedlings planted here are those selected in the selection ( S32). The selected ones may be planted as they are, or clones may be obtained from the selected ones by tissue culture or the like and used as seedlings. Here, for reference, this specification appropriately incorporates the content of "Tissue and Cell Culture and Propagation of Vegetables" (edited by the Editorial Committee of the Latest Biotechnology Encyclopedia) and the content of WO2020 / 196388. The timing of planting may be any time during the period from planting to harvesting as long as the cultivation of the cruciferous intergeneric hybrid is possible, and is not particularly limited. Preferably, it is from May to October.

[0028] <Cultivation (S34)> In cultivation, cruciferous intergeneric hybrids are cultivated. The place for cultivation is not particularly limited as long as it is a place where vegetable cultivation is possible. Examples include fields, greenhouses, plant factories, etc. The means for cultivation is not particularly limited, and it may be open-field cultivation or protected cultivation, and it may be It may be soil cultivation or hydroponics. What this step does not exclude is the implementation of operations generally carried out in plant cultivation. Examples include fertilization, pesticide spraying, pest control, leaf raking, bud raking, etc.

[0029] <Harvest (S35)> In harvesting, at least the leaves of the Brassica intergeneric hybrid are harvested. If necessary, flower buds, flower stalks, roots, etc. may also be harvested. The means for harvesting the leaves of the Brassica intergeneric hybrid is not particularly limited. The timing of harvesting varies depending on the purpose of use of the harvest target, and thus is not limited. Generally, the indicator for determining the harvest date is the size of the harvest target. On the other hand, depending on the purpose of use of the harvest target, the content of a specific component, etc. may be used as an indicator. Preferably, it is within 150 days from the planting date. If it exceeds 150 days from the planting date, the stem of the Brassica intergeneric hybrid becomes lignified and hardens, which is not preferable.

Examples

[0030] Hereinafter, the present invention will be described more specifically by way of examples, but the present invention is not limited to the following examples.

[0031] [Test 1. Understanding the relationship between the height of the growing point and the number of days until bolting is confirmed] [Materials and methods] What was used in Test 1 was an intergeneric hybrid (variety registration application number: No. 33291, name of the applied variety: Santeveil 48) with kale as the pollen parent and radish as the stigma parent. For 209 cloned seedlings produced at the same time, the height of the growing point was measured. The time when the height of the growing point was measured was the 29th day after acclimatization. After measuring the height of the growing point, planting was carried out. After planting, cultivation was carried out, and the number of days from planting until bolting was confirmed was measured. The confirmation of bolting was ​ It was carried out by the following method. Visually observe the growth point of the main stem to confirm the presence or absence of flower stalk formation. Those with flower stalks formed were regarded as those with bolting confirmed.

[0032] <Result> The average height of the growth points of 209 cloned seedlings used in Test 1 was 1.8 cm. The average number of days until bolting was confirmed for 209 cloned seedlings used in Test 1 was 69 days. There was.

[0033] [Table 1]

[0034] Table 1 shows the relationship between the height of the growth point and the average number of days until bolting is confirmed. It can be seen from this result that the following is true. The lower the height of the growth point, the longer the average number of days until bolting is confirmed. That is, by selecting and planting those with a lower height of the growth point, it is possible to suppress bolting.

[0035] [Table 2]

[0036] Table 2 shows the relationship between the average value of the height of the growth point and the number of days until bolting is confirmed. It can be seen from this result that the following is true. For those with a height of the growth point less than the average value of the cloned seedlings produced at the same time, the average number of days until bolting is confirmed is longer than that of those with a height above the average value. That is, by selecting those with a height of the growth point less than the average value of the cloned seedlings produced at the same time, it is possible to suppress bolting.

[0037] [Test 2. Understanding the relationship between the degree of bolting and the weight of the harvested product] <Materials and Methods> For Test 2, an intergeneric hybrid (variety registration application number: 33291, variety name: Santéveil 48) with kale as the pollen parent and radish as the stigma parent was used. The growing points of seedlings with low growing points were excised, and 18 cloned seedlings obtained through culture were used as Example 1. 20 cloned seedlings obtained from randomly excised growing points and produced at the same time as Example 1 were used as Comparative Example 1. Example 1 and Comparative Example 1 were planted, and the cultivation of the planted seedlings was carried out. Every two weeks after planting, the number of seedlings with bolting confirmed was measured. In addition, for any 3 seedlings of Example 1 and Comparative Example 1, at the 12th week after planting, the above-ground parts were sorted into good leaves, yellowing leaves, and stems (including flower stems. The same applies hereinafter.), and the weight ratios of each were calculated. The calculation formulas are shown below. Note that good leaves refer to leaves that are not yellowing. Among the harvested products, only good leaves can be shipped. Wish number: 33291, Name of the applied variety: Santéveil 48). The growing points of seedlings with low growing points were excised, and 18 cloned seedlings obtained through culture were used as Example 1. 20 cloned seedlings obtained from randomly excised growing points and produced at the same time as Example 1 were used as Comparative Example 1. Example 1 and Comparative Example 1 were planted, and the cultivation of the planted seedlings was carried out. Every two weeks after planting, the number of seedlings with bolting confirmed was measured. In addition, for any 3 seedlings of Example 1 and Comparative Example 1, at the 12th week after planting, the above-ground parts were sorted into good leaves, yellowing leaves, and stems (including flower stems. The same applies hereinafter.), and the weight ratios of each were calculated. The calculation formulas are shown below. Note that good leaves refer to leaves that are not yellowing. Among the harvested products, only good leaves can be shipped. Good leaf weight ratio = Good leaf weight (kg) / Above-ground part weight (kg) × 100 Yellowing leaf weight ratio = Yellowing leaf weight (kg) / Above-ground part weight (kg) × 100 Stem weight ratio = Stem weight (kg) / Above-ground part weight (kg) × 100 Note that good leaves refer to leaves that are not yellowing. Among the harvested products, only good leaves can be shipped. Among the harvested products, only good leaves can be shipped.

[0038] Good leaf weight ratio = Good leaf weight (kg) / Above-ground part weight (kg) × 100 Yellowing leaf weight ratio = Yellowing leaf weight (kg) / Above-ground part weight (kg) × 100 Stem weight ratio = Stem weight (kg) / Above-ground part weight (kg) × 100

[0039] <Results>

Table 3

[0040] Table 3 shows the relationship between the number of weeks after planting and the number of plants that bolted in Example 1 and Comparative Example 1. From this result, it can be seen that in Comparative Example 1, plants that bolted appeared from the 8th week after planting. Furthermore, in Comparative Example 1, at the 12th week after planting, bolting occurred in more than 80% of the plants. On the other hand, in Example 1, no plants bolted within 12 weeks after planting. In Example 1, no plants bolted within 12 weeks after planting. , in Example 1, bolting did not occur even after 12 weeks from transplantation. That is, in Example 1, bolting was suppressed compared to Comparative Example 1.

[0041]

Table 4

[0042] Table 4 shows the weight ratios of good leaves, yellowing leaves, and stems at 12 weeks after transplantation in Example 1 and Comparative Example 1. From this result, the following can be understood. In Example 1, compared to Comparative Example 1 , the weight ratio of good leaves is high and the weight ratio of stems is low. This is presumably because bolting occurred in Comparative Example 1 , increasing the weight of reproductive organs such as flower stalks. That is, when bolting occurs , the weight ratio of marketable leaves decreases.

[0043] [Test 3. Understanding the relationship between the height of the growth point and plant height] <Materials and Methods> For Test 3, an intergeneric hybrid (variety registration application number: No. 33291, name of the applied variety: Santeveil 48) with kale as the pollen parent and radish as the stigma parent was used. For 15 cloned seedlings produced at the same time, the height of the growth point [A] and plant height B] were measured on the 29th day after acclimatization.

[0044] <Results>

Table 5

[0045] Table 5 shows the relationship between the height of the growth point and plant height at 29 days after acclimatization. From this result, the following can be understood. Seedlings with a low growth point height tend to have a large value of plant height [B] / growth point height [A] . That is, seedlings with a large value of plant height [B] / growth point height [A] ​ By selecting [them], it becomes possible to prevent bolting or delay bolting. Specifically, By selecting individuals in which the value of plant height [B] / height of the growing point [A] is 8.5 or more, the height of the growing point is low (less than the average value of the population of Brassica intergeneric hybrids produced at the same time). It becomes possible to select individuals.

[0046] Furthermore, Table 5 shows the distribution of plant height at the 29th day after acclimation. It can be seen from this result that the following is the case. When the 29th day after acclimation is specified from another aspect, it is the period when the plant height is within the range of 6 cm to 19 c m (average value ± 3σ).

Industrial Applicability

[0047] The field in which the present invention is useful is the production of vegetables.

Claims

1. A method for suppressing bolting in Brassica intergeneric hybrids, which comprises at least the following steps: Selection: The Brassica intergeneric hybrid seedlings selected here, The index for said selection is the height of the growing point.

2. The method according to Claim 1, The Brassica intergeneric hybrid seedlings selected by said selection are those with a low height of the growing point.

3. The method according to Claim 2, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than the average value of the population of Brassica intergeneric hybrid seedlings produced at the same time.

4. The method according to Claim 3, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than 1.8 cm.

5. The method according to Claim 1, The index for said selection further includes plant height.

6. The method according to Claim 5, The relationship between [A] the height of the growing point (cm) and [B] the plant height (cm) satisfied by the selected Brassica intergeneric hybrid seedlings is B / A ≥ 8.

5.

7. The method according to any one of Claims 1 to 6, which further comprises the following step: Acclimatization: The Brassica intergeneric hybrid seedlings acclimatized here, The time for said selection is from the 4th week to the 5th week after acclimatization.

8. A method for producing Brassica intergeneric hybrid seedlings, which comprises at least the following steps: Selection: The Brassica intergeneric hybrid seedlings selected here, The index for said selection is the height of the growing point.

9. The production method according to Claim 8, The Brassica intergeneric hybrid seedlings selected by said selection are those with a low height of the growing point.

10. The production method according to Claim 9, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than the average value of the population of Brassica intergeneric hybrid seedlings produced at the same time.

11. The production method according to Claim 10, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than 1.8 cm.

12. The production method according to Claim 8, The index for said selection further includes plant height.

13. The production method according to Claim 12, The relationship between [A] the height of the growing point (cm) and [B] the plant height (cm) satisfied by the selected Brassica intergeneric hybrid seedlings is B / A ≥ 8.

5.

14. The production method according to Claim 8, The index for said selection further includes plant height.

15. The production method according to Claim 14, The relationship between [A] the height of the growing point (cm) and [B] the plant height (cm) satisfied by the selected Brassica intergeneric hybrid seedlings is B / A ≥ 8.

5.

16. The production method according to any one of Claims 8 to 15, which further comprises the following step: Acclimatization: The Brassica intergeneric hybrid seedlings acclimatized here, The time for said selection is from the 4th week to the 5th week after acclimatization.

17. A method for suppressing bolting in Brassica intergeneric hybrids, which comprises at least the following steps: Selection: The Brassica intergeneric hybrid seedlings selected here, The index for said selection is the height of the growing point.

18. The method according to Claim 17, The Brassica intergeneric hybrid seedlings selected by said selection are those with a low height of the growing point.

19. The method according to Claim 18, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than the average value of the population of Brassica intergeneric hybrid seedlings produced at the same time.

20. The method according to Claim 19, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than 1.8 cm.

21. The method according to Claim 17, The index for said selection further includes plant height.

22. The method according to Claim 21, The relationship between [A] the height of the growing point (cm) and [B] the plant height (cm) satisfied by the selected Brassica intergeneric hybrid seedlings is B / A ≥ 8.

5.

23. The method according to any one of Claims 17 to 22, which further comprises the following step: Acclimatization: The Brassica intergeneric hybrid seedlings acclimatized here, The time for said selection is from the 4th week to the 5th week after acclimatization.

24. A method for suppressing bolting in Brassica intergeneric hybrids, which comprises at least the following steps: Selection: The Brassica intergeneric hybrid seedlings selected here, The index for said selection is the height of the growing point.

25. The method according to Claim 24, The Brassica intergeneric hybrid seedlings selected by said selection are those with a low height of the growing point.

26. The method according to Claim 25, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than the average value of the population of Brassica intergeneric hybrid seedlings produced at the same time.

27. The method according to Claim 26, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than 1.8 cm.

28. The method according to Claim 24, The index for said selection further includes plant height.

29. The method according to Claim 28, The relationship between [A] the height of the growing point (cm) and [B] the plant height (cm) satisfied by the selected Brassica intergeneric hybrid seedlings is B / A ≥ 8.

5.

30. The method according to any one of Claims 24 to 29, which further comprises the following step: Acclimatization: The Brassica intergeneric hybrid seedlings acclimatized here, The time for said selection is from the 4th week to the 5th week after acclimatization.

31. A method for suppressing bolting in Brassica intergeneric hybrids, which comprises at least the following steps: Selection: The Brassica intergeneric hybrid seedlings selected here, The index for said selection is the height of the growing point.

32. The method according to Claim 31, The Brassica intergeneric hybrid seedlings selected by said selection are those with a low height of the growing point.

33. The method according to Claim 32, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than the average value of the population of Brassica intergeneric hybrid seedlings produced at the same time.

34. The method according to Claim 33, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than 1.8 cm.

35. The method according to Claim 31, The index for said selection further includes plant height.

36. The method according to Claim 35, The relationship between [A] the height of the growing point (cm) and [B] the plant height (cm) satisfied by the selected Brassica intergeneric hybrid seedlings is B / A ≥ 8.

5.

37. The method according to any one of Claims 31 to 36, which further comprises the following step: Acclimatization: The Brassica intergeneric hybrid seedlings acclimatized here, The time for said selection is from the 4th week to the 5th week after acclimatization.

38. A method for suppressing bolting in Brassica intergeneric hybrids, which comprises at least the following steps: Selection: The Brassica intergeneric hybrid seedlings selected here, The index for said selection is the height of the growing point.

39. The method according to Claim 38, The Brassica intergeneric hybrid seedlings selected by said selection are those with a low height of the growing point.

40. The method according to Claim 39, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than the average value of the population of Brassica intergeneric hybrid seedlings produced at the same time.

41. The method according to Claim 40, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than 1.8 cm.

42. The method according to Claim 38, The index for said selection further includes plant height.

43. The method according to Claim 42, The relationship between [A] the height of the growing point (cm) and [B] the plant height (cm) satisfied by the selected Brassica intergeneric hybrid seedlings is B / A ≥ 8.

5.

44. The method according to any one of Claims 38 to 43, which further comprises the following step: Acclimatization: The Brassica intergeneric hybrid seedlings acclimatized here, The time for said selection is from the 4th week to the 5th week after acclimatization.

45. A method for suppressing bolting in Brassica intergeneric hybrids, which comprises at least the following steps: Selection: The Brassica intergeneric hybrid seedlings selected here, The index for said selection is the height of the growing point.

46. The method according to Claim 45, The Brassica intergeneric hybrid seedlings selected by said selection are those with a low height of the growing point.

47. The method according to Claim 46, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than the average value of the population of Brassica intergeneric hybrid seedlings produced at the same time.

48. The method according to Claim 47, The height of the growing point of the Brassica intergeneric hybrid seedlings selected by said selection is less than 1.8 cm.

49. The method according to Claim 45, The index for said selection further includes plant height.

50. The method according to Claim 49, The relationship between [A] the height of the growing point (cm) and [B] the plant height (cm) satisfied by the selected Brassica intergeneric hybrid seedlings is B / A ≥ 8.

5.

51. The method The production method according to any one of claims 8 to 13, which further comprises the following steps as follows: Acclimatization: The seedlings of the Brassica intergeneric hybrid are acclimatized here, and the selection period is from the 4th week to the 5th week after acclimatization.

15. A production method for Brassica intergeneric hybrid vegetables, which comprises at least the following steps: Selection: The seedlings of the Brassica intergeneric hybrid are selected here, and the selection criterion is the height of the growing point. Planting: The seedlings of the Brassica intergeneric hybrid selected by the above selection are planted here and Harvesting: At least the leaves of the Brassica intergeneric hybrid are harvested here.

16. The production method according to claim 15, wherein the seedlings of the Brassica intergeneric hybrid selected by the above selection are those with a low height of the growing point.

17. The production method according to claim 16, wherein the height of the growing point of the seedlings of the Brassica intergeneric hybrid selected by the above selection is less than the average value of the group of seedlings of the Brassica intergeneric hybrid produced at the same time and

18. The production method according to claim 17, wherein the height of the growing point of the seedlings of the Brassica intergeneric hybrid selected by the above selection is less than 1.8 cm and

19. The production method according to claim 15, wherein the selection criterion further includes plant height.

20. The production method according to claim 19, wherein the relationship between [A] the height of the growing point (cm) and [B] the plant height (cm) satisfied by the selected seedlings of the Brassica intergeneric hybrid is B / A ≥ 8.

5.

21. The production method according to any one of claims 15 to 20, which further comprises the following steps as follows: Acclimatization: The seedlings of the Brassica intergeneric hybrid are acclimatized here, and the selection period is from the 4th week to the 5th week after acclimatization. ​

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