SNP markers for predicting high water temperature tolerance of olive flounder and uses thereof

Abstract

To provide an SNP marker composition for predicting high water temperature tolerance of olive flounder, and a method for predicting high water temperature tolerance of olive flounder using the same.SOLUTION: An SNP marker composition for predicting high water temperature tolerance of olive flounder according to the present disclosure includes one or more selected from the group consisting of polynucleotides composed of 10 to 100 consecutive nucleotides, or polynucleotides complementary thereto, each containing a predetermined SNP at a predetermined position in a polynucleotide composed of a nucleotide sequence of SEQ ID NOs: 1 to 30. The SNP marker composition of the present disclosure can be utilized for genetic selection of olive flounder having tolerance to high water temperature, and can promote development of high water temperature-tolerant varieties and sustainable production.SELECTED DRAWING: Figure 4a

Description

[Technical Field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application is Korean Patent Application No. 2021-0116084 filed on August 31, 2021. No. 60 / 629,993, filed on Oct. 1, 2007, the contents of which are incorporated herein by reference in their entirety. will be done.

[0002] This disclosure provides a method for predicting high water temperature tolerance (HWTT) of flounder. The present invention relates to a SNP marker composition and a method for predicting high water temperature tolerance of Japanese flounder using the same. do. [Background technology]

[0003] In the case of South Korea, most of the farmed flounder is produced in Jeju Special Self-Governing Province and Jeollanam-do, and More than 40,000 tons of farmed flounder are produced and distributed annually. In addition, in land-based aquaculture farms or net cage farms that use natural seawater, seawater temperature is an important factor in aquaculture production. The longer the high water temperature period in summer due to climate change is maintained, the greater the aquaculture productivity. It is known that glitter can cause various problems, such as illness and weakening of the immune system. In response to this, new varieties that can withstand high water temperatures are needed, and in order to develop such varieties, To achieve this, genetic selective breeding based on a method that can select flounder that can tolerate high water temperatures is necessary. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Korean Patent No. 10-2080120 Summary of the Invention [Problem to be solved by the invention]

[0005] The object of the present disclosure is to provide a SNP marker composition for predicting high water temperature tolerance in Japanese flounder, and to detect or A composition containing an amplifiable formulation, a kit for predicting high water temperature tolerance of flounder containing the composition, and To provide a method for predicting the tolerance of flounder to high water temperatures. [Means for solving the problem]

[0006] In order to achieve the above object, the present disclosure provides a polynucleotide consisting of the base sequence of SEQ ID NO: 1. The 36th base of the nucleotide is G or A, and the 10 to 10 a polynucleotide consisting of 0 consecutive bases or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 2 is A or G a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 3 The 36th base of the nucleotide is T or C, and 10 to 14 A polynucleotide consisting of 100 consecutive bases or a polynucleotide complementary thereto nucleotide: the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 4 is A or or G, and is composed of 10 to 100 consecutive bases including the 36th base a polynucleotide having the base sequence of SEQ ID NO: 5 or a polynucleotide complementary thereto; The 36th base of the polynucleotide is T or G, A polynucleotide consisting of 10 to 100 consecutive bases containing 36 of the polynucleotide consisting of the base sequence of SEQ ID NO: 6 the 36th base is C or A, and 10 to 100 consecutive bases including the 36th base are A polynucleotide consisting of the bases or a polynucleotide complementary thereto; SEQ ID NO: The 36th base of the polynucleotide consisting of the 7 base sequence is T or G, Polynucleotides consisting of 10 to 100 consecutive bases including the sixth base or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 8 The 36th base of the nucleotide is A or G, and the 10 to 10 A polynucleotide consisting of 0 consecutive bases or a polynucleotide complementary thereto Polynucleotide consisting of the base sequence of SEQ ID NO: 9, the 36th base of which is T or C and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. Nucleotide or a polynucleotide complementary thereto; consisting of the base sequence of SEQ ID NO: 10 The 36th base of the polynucleotide to be detected is G or A, and the 36th base is A polynucleotide consisting of 10 to 100 consecutive bases or a complementary Polynucleotide: Polynucleotide 36 consisting of the base sequence of SEQ ID NO: 11 is T or C, and 10 to 100 consecutive bases including the 36th base A polynucleotide consisting of the group or a polynucleotide complementary thereto; SEQ ID NO: 1 The 36th base of the polynucleotide consisting of the base sequence of 2 is T or C, A polynucleotide consisting of 10 to 100 consecutive bases, including the 36th base. or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 13 The 36th base of the nucleotide is A or G, and 10 to 14 bases including the 36th base are A polynucleotide consisting of 100 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 14 is C or T, and consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide having the base sequence of SEQ ID NO: 15 or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of a sequence of A and G is A or G, A polynucleotide consisting of 10 to 100 consecutive bases containing the base or a polynucleotide complementary to the base sequence of SEQ ID NO: 16; the 36th base is A or C, and 10 to 100 A polynucleotide consisting of consecutive bases or a polynucleotide complementary thereto; Sequence number: The 36th base of the polynucleotide consisting of a 17-base sequence is A or C. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; the polynucleotide is composed of the base sequence of SEQ ID NO: 18 the 36th base of the polynucleotide to be prepared is T or C, A polynucleotide consisting of 10 to 100 consecutive bases or a complementary polynucleotide Polynucleotide: The 36th polynucleotide consisting of the base sequence of SEQ ID NO: 19 The base is A or C, and 10 to 100 consecutive bases including the 36th base or a polynucleotide complementary thereto; SEQ ID NO: 20 The 36th base of the polynucleotide consisting of the base sequence of Polynucleotides consisting of 10 to 100 consecutive bases including the sixth base or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 21 the 36th base of the nucleic acid is G or T, and A polynucleotide consisting of 00 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 22 is T. or C, and is composed of 10 to 100 consecutive bases including the 36th base a polynucleotide having the base sequence of SEQ ID NO: 23 or a polynucleotide complementary thereto; The 36th base of the polynucleotide is C or A, A polynucleotide consisting of 10 to 100 consecutive bases containing the base or Complementary polynucleotide: a polynucleotide consisting of the base sequence of SEQ ID NO: 24 The 36th base is A or C, and 10 to 100 consecutive sequences containing the 36th base are a polynucleotide consisting of the following bases or a polynucleotide complementary thereto; Number: The 36th base of the polynucleotide consisting of a 25-base sequence is T or C. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; comprising the base sequence of SEQ ID NO: 26 the 36th base of the polynucleotide is T or C, and A polynucleotide consisting of 100 to 100 consecutive bases or a polynucleotide complementary thereto Nucleotide; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 27 The base is T or C, and the base is 10 to 100 consecutive bases including the 36th base. A polynucleotide constructed as above or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence is C or T, and the 36th base A polynucleotide consisting of 10 to 100 consecutive bases containing the bases or a polynucleotide corresponding thereto Complementary polynucleotide: the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 29 is A or G, and the base is 10 to 100 consecutive bases including the 36th base. or a polynucleotide complementary thereto; and a salt of SEQ ID NO: 30 The 36th base of the polynucleotide consisting of the base sequence is T or G, A polynucleotide or a corresponding polynucleotide consisting of 10 to 100 consecutive bases containing A method for detecting a high-water fish containing one or more polynucleotides selected from the group consisting of: A SNP (single nucleotide polymorphism) marker composition for predicting temperature tolerance is provided.

[0007] When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 1 is A, In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 2 is A In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 3 is T, When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 4 is A, In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 5 is G: the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 6 is C; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 7 is When the base is T, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 8 When the base is A, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 9 When the base is C, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 10 When the base is A, the polynucleotide consisting of the base sequence of SEQ ID NO: 11 When the 36th base is T, the polynucleotide consisting of the base sequence of SEQ ID NO: 12 When the 36th base of the polynucleotide is T, the polynucleotide having the base sequence of SEQ ID NO: 13 is When the 36th base of the nucleotide is A, the polynucleotide having the base sequence of SEQ ID NO: 14 When the 36th base of the nucleotide is C, the polypeptide consisting of the base sequence of SEQ ID NO: 15 When the 36th base of the oligonucleotide is G, the polynucleotide having the base sequence of SEQ ID NO: 16 When the 36th base of the nucleotide is A, the nucleotide sequence of SEQ ID NO: 17 is When the 36th base of the polynucleotide is A, the polynucleotide has the base sequence of SEQ ID NO: 18. When the 36th base of the polynucleotide is C, the polynucleotide has the base sequence of SEQ ID NO: 19. When the 36th base of the polynucleotide is A, the polynucleotide has the base sequence of SEQ ID NO: 20. When the 36th base of the polynucleotide is A, the polynucleotide has the base sequence of SEQ ID NO: 21. When the 36th base of the polynucleotide is T, the polynucleotide has the base sequence of SEQ ID NO: 22. When the 36th base of the polynucleotide is T, In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 24 is A: the 36th base of the polynucleotide consisting of SEQ ID NO: 25 is A; When the 36th base of the polynucleotide consisting of the base sequence is C, the polynucleotide of SEQ ID NO: 26 The 36th base of the polynucleotide having the base sequence of SEQ ID NO: When the 36th base of the polynucleotide consisting of the 27-base sequence is T, Code: When the 36th base of a polynucleotide consisting of a 28-base sequence is C; Sequence number: When the 36th base of a polynucleotide consisting of a 29-base sequence is G or the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 30 If T, it can be predicted that the flounder is resistant to high water temperatures.

[0008] The present disclosure also provides a formulation capable of detecting or amplifying SNP markers for predicting high water temperature tolerance in flounder. A composition for predicting high water temperature tolerance of flounder, comprising the SNP marker of SEQ ID NO: 1. The 36th base of the polynucleotide consisting of the base sequence of Polynucleotides consisting of 10 to 100 consecutive bases including the sixth base or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 2 The 36th base of the nucleotide is A or G, and the 10 to 10 A polynucleotide consisting of 0 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 3 is T or C, and a polypeptide consisting of 10 to 100 consecutive bases including the 36th base a polynucleotide or a polynucleotide complementary thereto; comprising the base sequence of SEQ ID NO: 4 The 36th base of the polynucleotide to be detected is A or G, and the 36th base is A polynucleotide consisting of 10 to 100 consecutive bases or a complementary a polynucleotide having the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 5; is T or G, and 10 to 100 consecutive bases including the 36th base a polynucleotide consisting of the group or a polynucleotide complementary thereto; SEQ ID NO: 6 The 36th base of the polynucleotide consisting of the base sequence of Polynucleotides consisting of 10 to 100 consecutive bases including the sixth base or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 7 The 36th base of the nucleotide is T or G, and the 10 to 10 A polynucleotide consisting of 0 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 8 is A or G, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base a polynucleotide or a polynucleotide complementary thereto; comprising the base sequence of SEQ ID NO: 9 The 36th base of the polynucleotide to be detected is T or C, and the 36th base is A polynucleotide consisting of 10 to 100 consecutive bases or a complementary a polynucleotide having the base sequence of SEQ ID NO: 10; the 36th base is G or A, and 10 to 100 consecutive bases including the 36th base are A polynucleotide consisting of the bases or a polynucleotide complementary thereto; SEQ ID NO: The 36th base of the polynucleotide consisting of an 11-base sequence is T or C, and Polynucleotides consisting of 10 to 100 consecutive bases, including the 36th base or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 12 The 36th base of the nucleotide is T or C, and the 36th base is within 10 A polynucleotide consisting of 100 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 13 is A or G, consisting of 10 to 100 consecutive bases including the 36th base a polynucleotide having the sequence shown in SEQ ID NO: 14 or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the sequence is C or T, A polynucleotide consisting of 10 to 100 consecutive bases containing the base A polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 15 the 36th base of the nucleotide is A or G, and 10 to 100 nucleotides including the 36th base a polynucleotide consisting of consecutive bases of the above or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 16 is A or C and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. Nucleotide or a polynucleotide complementary thereto; consisting of the base sequence of SEQ ID NO: 17 The 36th base of the polynucleotide to be detected is A or C, and the 36th base is A polynucleotide consisting of 10 to 100 consecutive bases or a complementary a polynucleotide having the base sequence of SEQ ID NO: 18; the 36th base is T or C, and 10 to 100 consecutive bases including the 36th base are A polynucleotide consisting of the bases or a polynucleotide complementary thereto; SEQ ID NO: The 36th base of the polynucleotide consisting of a 19-base sequence is A or C, and Polynucleotides consisting of 10 to 100 consecutive bases, including the 36th base or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 20 The 36th base of the nucleotide is G or A, and there are 10 or less bases including the 36th base. A polynucleotide consisting of 100 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 21 is G or T, consisting of 10 to 100 consecutive bases including the 36th base a polynucleotide having the sequence shown in SEQ ID NO: 22 or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the sequence is T or C, A polynucleotide consisting of 10 to 100 consecutive bases containing the base A polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 23 the 36th base of the nucleotide is C or A, and 10 to 100 nucleotides including the 36th base a polynucleotide consisting of consecutive bases of the above or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 24 is A or C and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. Nucleotide or a polynucleotide complementary thereto; consisting of the base sequence of SEQ ID NO: 25 The 36th base of the polynucleotide to be detected is T or C, and the 36th base is A polynucleotide consisting of 10 to 100 consecutive bases or a complementary a polynucleotide having the base sequence of SEQ ID NO: 26; the 36th base is T or C, and 10 to 100 consecutive bases including the 36th base are A polynucleotide consisting of the bases or a polynucleotide complementary thereto; SEQ ID NO: The 36th base of the polynucleotide consisting of a 27-base sequence is T or C, and Polynucleotides consisting of 10 to 100 consecutive bases, including the 36th base or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 28 The 36th base of the nucleotide is C or T, and the 36th base is within 10 A polynucleotide consisting of 100 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 29 is A or G, and consisting of 10 to 100 consecutive bases including the 36th base a polynucleotide having the sequence shown in SEQ ID NO: 30 or a polynucleotide complementary thereto; the 36th base of the polynucleotide consisting of the sequence is T or G, or a polynucleotide complementary thereto, which consists of 10 to 100 consecutive bases containing High water temperature tolerance of Japanese flounder, containing one or more polynucleotides selected from the group consisting of A predictive composition is provided.

[0009] The formulation may contain primers or probes capable of detecting or amplifying the SNP markers. There is a possibility.

[0010] The present disclosure also provides a method for predicting high water temperature resistance of flounder, comprising the composition for predicting high water temperature resistance of flounder. A kit for use is provided.

[0011] The present disclosure also provides (a) a method for predicting high water temperature tolerance of flounder from DNA of a sample isolated from the flounder. (b) amplifying or detecting polymorphic sites of the SNP markers; and (c) amplifying or detecting polymorphic sites of the SNP markers; determining the base of the polymorphic site amplified or detected in step (a), The NP marker is the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 1. The base is G or A, and the base is 10 to 100 consecutive bases including the 36th base. A polynucleotide constructed or a polynucleotide complementary thereto; a salt of SEQ ID NO: 2 The 36th base of the polynucleotide consisting of the base sequence is A or G, A polynucleotide consisting of 10 to 100 consecutive bases containing the base A polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 3 the 36th base of the nucleotide is T or C, and 10 to 100 nucleotides including the 36th base a polynucleotide consisting of consecutive bases of the above or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 4 is A or G. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; comprising the base sequence of SEQ ID NO: 5 the 36th base of the polynucleotide is T or G, A polynucleotide consisting of 0 to 100 consecutive bases or a complementary polynucleotide Polynucleotide; the 36th base of a polynucleotide consisting of the base sequence of SEQ ID NO: 6 base is C or A, and the base is 10 to 100 consecutive bases including the 36th base A polynucleotide constructed as above or a polynucleotide complementary thereto; a salt of SEQ ID NO: 7 The 36th base of the polynucleotide consisting of the base sequence is T or G, A polynucleotide consisting of 10 to 100 consecutive bases containing the base A polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 8 the 36th base of the nucleotide is A or G, and 10 to 100 nucleotides including the 36th base a polynucleotide consisting of consecutive bases of the above or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 9 is T or C. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; comprising the base sequence of SEQ ID NO: 10 The 36th base of the polynucleotide to be prepared is G or A, and A polynucleotide consisting of 10 to 100 consecutive bases or a complementary polynucleotide Polynucleotide: The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 11 is T or C, and 10 to 100 consecutive bases including the 36th base A polynucleotide consisting of the group or a polynucleotide complementary thereto; SEQ ID NO: 1 The 36th base of the polynucleotide consisting of the base sequence of 2 is T or C, A polynucleotide consisting of 10 to 100 consecutive bases, including the 36th base. or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 13 The 36th base of the nucleotide is A or G, and 10 to 14 bases including the 36th base are A polynucleotide consisting of 100 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 14 is C or T, and consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide having the base sequence of SEQ ID NO: 15 or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of a sequence of A and G is A or G, A polynucleotide consisting of 10 to 100 consecutive bases containing the base or a polynucleotide complementary to the base sequence of SEQ ID NO: 16; the 36th base is A or C, and 10 to 100 A polynucleotide consisting of consecutive bases or a polynucleotide complementary thereto; Sequence number: The 36th base of the polynucleotide consisting of a 17-base sequence is A or C. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; the polynucleotide is composed of the base sequence of SEQ ID NO: 18 the 36th base of the polynucleotide to be prepared is T or C, A polynucleotide consisting of 10 to 100 consecutive bases or a complementary polynucleotide Polynucleotide: The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 19 the base is A or C, and 10 to 100 consecutive salts including the 36th base a polynucleotide consisting of the group or a polynucleotide complementary thereto; SEQ ID NO: 2 The 36th base of the polynucleotide consisting of the base sequence of 0 is G or A, A polynucleotide consisting of 10 to 100 consecutive bases, including the 36th base. or a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 21 The 36th base of the nucleotide is G or T, and 10 to 14 bases including the 36th base are A polynucleotide consisting of 100 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 22 is T or C, and is composed of 10 to 100 consecutive bases including the 36th base. a polynucleotide having the base sequence of SEQ ID NO: 23 or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of a sequence of C or A is A polynucleotide consisting of 10 to 100 consecutive bases containing the base or a polynucleotide complementary to the base sequence of SEQ ID NO: 24; the 36th base is A or C, and 10 to 100 A polynucleotide consisting of consecutive bases or a polynucleotide complementary thereto; Sequence number: The 36th base of the polynucleotide consisting of the 25 base sequence is T or C. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; the polynucleotide is composed of the base sequence of SEQ ID NO: 26 the 36th base of the polynucleotide to be prepared is T or C, A polynucleotide consisting of 10 to 100 consecutive bases or a complementary polynucleotide Polynucleotide: The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 27 is T or C, and 10 to 100 consecutive bases including the 36th base a polynucleotide consisting of the group or a polynucleotide complementary thereto; SEQ ID NO: 2 The 36th base of a polynucleotide consisting of an 8-base sequence is C or T, and A polynucleotide consisting of 10 to 100 consecutive bases including the base 1, or a polynucleotide complementary to the nucleotide sequence of SEQ ID NO: 29; The base is A or G, and 10 to 100 consecutive bases including the 36th base or a polynucleotide complementary thereto; and SEQ ID NO: 30 the 36th base of the polynucleotide having the base sequence A polynucleotide consisting of 10 to 100 consecutive bases containing the bases or a polynucleotide corresponding thereto A method for detecting a high-water fish containing one or more polynucleotides selected from the group consisting of: A composition for predicting temperature tolerance is provided.

[0012] When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 1 is A, In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 2 is A In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 3 is T, When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 4 is A, In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 5 is G the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 6 is C; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 7 is When the base is T, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 8 When the base is A, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 9 When the base is C, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 10 When the base is A, the polynucleotide consisting of the base sequence of SEQ ID NO: 11 When the 36th base is T, the polynucleotide consisting of the base sequence of SEQ ID NO: 12 When the 36th base of the polynucleotide sequence of SEQ ID NO: 13 is T, When the 36th base of the polynucleotide is A, the polynucleotide having the base sequence of SEQ ID NO: 14 When the 36th base of the nucleotide is C, the polynucleotide having the base sequence of SEQ ID NO: 15 When the 36th base of the nucleotide is G, a polynucleotide having the base sequence of SEQ ID NO: 16 is prepared. When the 36th base of the nucleotide is A, a polynucleotide consisting of the base sequence of SEQ ID NO: 17 When the 36th base of the nucleotide is A, the polypeptide consisting of the base sequence of SEQ ID NO: 18 When the 36th base of the nucleotide is C, the nucleotide sequence of SEQ ID NO: 19 is When the 36th base of the polynucleotide is A, the base sequence of SEQ ID NO: 20 is In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 21 is A: the 36th base of the polynucleotide consisting of SEQ ID NO: 22 is T; When the 36th base of the polynucleotide consisting of the base sequence is T, the polynucleotide of SEQ ID NO: 23 The 36th base of the polynucleotide having the base sequence of SEQ ID NO: When the 36th base of a polynucleotide consisting of a 24-base sequence is A, No. 36 of a polynucleotide consisting of a 25-base sequence is C; Sequence number: When the 36th base of a polynucleotide consisting of a 26-base sequence is T; When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 27 is T the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 28 is C; In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 29 is G; or the 3'-nucleotide sequence of the polynucleotide consisting of the base sequence of SEQ ID NO: 30 If the sixth base is T, it can be predicted that the flounder is resistant to high water temperatures.

[0013] The present disclosure also relates to a kit for predicting high water temperature resistance of flounder; and a method for predicting high water temperature resistance of flounder. The present invention provides a use of the aforementioned SNP marker composition for predicting high water temperature tolerance in flounder. [Effects of the Invention]

[0014] The SNP marker composition of the present disclosure can be used for the genetic selection of flounder with high water temperature tolerance. It can be used for section. [Brief explanation of the drawings]

[0015] [Figure 1] Figure 1 is a graph showing the cumulative mortality rate with changes in water temperature in the high water temperature tolerance experiment. [Figure 2a] Figure 2 shows the GWAS analysis of high water temperature tolerance by type (survival, survival days, and survival time). [Figure 2b] See brief description of Figure 2a. [Figure 2c] See brief description of Figure 2a. [Figure 3a] 3 to 32 show the phenotype distribution for the genotypes of 30 SNPs for predicting high water temperature tolerance. [Figure 3b] See brief description of Figure 3a. [Figure 3c] See brief description of Figure 3a. [Figure 4a] See brief description of Figure 3a. [Figure 4b] See brief description of Figure 3a. [Figure 4c] See brief description of Figure 3a. [Figure 5a] See brief description of Figure 3a. [Figure 5b] See brief description of Figure 3a. [Figure 5c] See brief description of Figure 3a. [Figure 6a] See brief description of Figure 3a. [Figure 6b] See brief description of Figure 3a. [Figure 6c] See brief description of Figure 3a. [Figure 7a] See brief description of Figure 3a. [Figure 7b] See brief description of Figure 3a. [Figure 7c] See brief description of Figure 3a. [Figure 8a] See brief description of Figure 3a. [Figure 8b] See brief description of Figure 3a. [Figure 8c] See brief description of Figure 3a. [Figure 9a] See brief description of Figure 3a. [Figure 9b] See brief description of Figure 3a. [Figure 9c] See brief description of Figure 3a. [Figure 10a] See brief description of Figure 3a. [Figure 10b] See brief description of Figure 3a. [Figure 10c] See brief description of Figure 3a. [Figure 11a] See brief description of Figure 3a. [Figure 11b] See brief description of Figure 3a. [Figure 11c] See brief description of Figure 3a. [Figure 12a] See brief description of Figure 3a. [Figure 12b] See brief description of Figure 3a. [Figure 12c] See brief description of Figure 3a. [Figure 13a] See brief description of Figure 3a. [Figure 13b] See brief description of Figure 3a. [Figure 13c] See brief description of Figure 3a. [Figure 14a] See brief description of Figure 3a. [Figure 14b] See brief description of Figure 3a. [Figure 14c] See brief description of Figure 3a. [Figure 15a] See brief description of Figure 3a. [Figure 15b] See brief description of Figure 3a. [Figure 15c] See brief description of Figure 3a. [Figure 16a] See brief description of Figure 3a. [Figure 16b] See brief description of Figure 3a. [Figure 16c] See brief description of Figure 3a. [Figure 17a] See brief description of Figure 3a. [Figure 17b] See brief description of Figure 3a. [Figure 17c] See brief description of Figure 3a. [Figure 18a] See brief description of Figure 3a. [Figure 18b] See brief description of Figure 3a. [Figure 18c] See brief description of Figure 3a. [Figure 19a] See brief description of Figure 3a. [Figure 19b] See brief description of Figure 3a. [Figure 19c] See brief description of Figure 3a. [Figure 20a] See brief description of Figure 3a. [Figure 20b] See brief description of Figure 3a. [Figure 20c] See brief description of Figure 3a. [Figure 21a] See brief description of Figure 3a. [Figure 21b] See brief description of Figure 3a. [Figure 21c]See brief description of Figure 3a. [Figure 22a] See brief description of Figure 3a. [Figure 22b] See brief description of Figure 3a. [Figure 22c] See brief description of Figure 3a. [Figure 23a] See brief description of Figure 3a. [Figure 23b] See brief description of Figure 3a. [Figure 23c] See brief description of Figure 3a. [Figure 24a] See brief description of Figure 3a. [Figure 24b] See brief description of Figure 3a. [Figure 24c] See brief description of Figure 3a. [Figure 25a] See brief description of Figure 3a. [Figure 25b] See brief description of Figure 3a. [Figure 25c] See brief description of Figure 3a. [Figure 26a] See brief description of Figure 3a. [Figure 26b] See brief description of Figure 3a. [Figure 26c] See brief description of Figure 3a. [Figure 27a] See brief description of Figure 3a. [Figure 27b] See brief description of Figure 3a. [Figure 27c] See brief description of Figure 3a. [Figure 28a] See brief description of Figure 3a. [Figure 28b] See brief description of Figure 3a. [Figure 28c] See brief description of Figure 3a. [Figure 29a] See brief description of Figure 3a. [Figure 29b] See brief description of Figure 3a. [Figure 29c] See brief description of Figure 3a. [Figure 30a] See brief description of Figure 3a. [Figure 30b] See brief description of Figure 3a. [Figure 30c] See brief description of Figure 3a. [Figure 31a] See brief description of Figure 3a. [Figure 31b] See brief description of Figure 3a. [Figure 31c] See brief description of Figure 3a. [Figure 32a] See brief description of Figure 3a. [Figure 32b] See brief description of Figure 3a. [Figure 32c] See brief description of Figure 3a. DETAILED DESCRIPTION OF THE INVENTION

[0016] The inventors have developed a SNP marker for predicting resistance to viral hemorrhagic septicemia in Japanese flounder. There is a history of filing a patent application for "(Korean Patent Registration No. 10-2281658)" The entire contents of the issued patents are incorporated by reference into this disclosure.

[0017] This disclosure is based on the results of a 70KSNP chip for flounder, which was selected in a high water temperature exposure experiment. Genetic analysis and genome-wide association study (GWAS) Through WAS, we identified single nucleotide polymorphisms (SNPs) that can predict high water temperature tolerance.

[0018] In one aspect, the present disclosure relates to a SNP marker composition for predicting high water temperature tolerance in flounder. is.

[0019] Specifically, the present disclosure relates to the 36th base of a polynucleotide consisting of the base sequence of SEQ ID NO: 1. is G or A, and 10 to 100 consecutive bases including the 36th base a polynucleotide consisting of the bases of SEQ ID NO: 2 or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the sequence is A or G, and A polynucleotide consisting of 10 to 100 consecutive bases or a polynucleotide complementary thereto Nucleotide: The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 3 is T or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base a polynucleotide or a polynucleotide complementary thereto; the polynucleotide is composed of the base sequence of SEQ ID NO: 4 The 36th base of the polynucleotide is A or G, and 10 A polynucleotide consisting of 100 to 100 consecutive bases or a polynucleotide complementary thereto Nucleotide: The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 5 is T or G, and is composed of 10 to 100 consecutive bases including the 36th base a polynucleotide prepared by the method of claim 1 or a polynucleotide complementary thereto; the base of SEQ ID NO: 6 The 36th base of the polynucleotide consisting of the sequence is C or A, A polynucleotide consisting of 10 to 100 consecutive bases containing the base A polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 7 the 36th base is T or G, and 10 to 100 A polynucleotide consisting of consecutive bases or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of sequence number 8 is A or G. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; comprising the base sequence of SEQ ID NO: 9 The 36th base of the polynucleotide is T or C, and 10 A polynucleotide consisting of 100 to 100 consecutive bases or a polynucleotide complementary thereto Nucleotide; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 10 The base is G or A, and the base is 10 to 100 consecutive bases including the 36th base. A polynucleotide constructed as above or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence is T or C, A polynucleotide consisting of 10 to 100 consecutive bases including the base a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 12 The 36th base of the nucleotide is T or C, and the 10 to 10 A polynucleotide consisting of 0 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 13 is A or is G, and is composed of 10 to 100 consecutive bases including the 36th base. A polynucleotide or a polynucleotide complementary thereto; the base sequence of SEQ ID NO: 14 The 36th base of the polynucleotide is C or T, and the 36th base A polynucleotide or a polynucleotide corresponding thereto, which is composed of 10 to 100 consecutive bases including Complementary polynucleotide: 3 of the polynucleotide consisting of the base sequence of SEQ ID NO: 15 The sixth base is A or G, and 10 to 100 consecutive bases including the 36th base are a polynucleotide consisting of the bases of SEQ ID NO: No.: The 36th base of a polynucleotide consisting of a 16-base sequence is A or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; consisting of the base sequence of SEQ ID NO: 17 The 36th base of the polynucleotide is A or C, and 10 A polynucleotide consisting of 100 to 100 consecutive bases or a polynucleotide complementary thereto Nucleotide; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 18 The base is T or C, and the base is 10 to 100 consecutive bases including the 36th base. A polynucleotide constructed as above or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence is A or C, A polynucleotide consisting of 10 to 100 consecutive bases including the base a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 20 The 36th base of the nucleotide is G or A, and the 10 to 10 A polynucleotide consisting of 0 consecutive bases or a polynucleotide complementary thereto The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 21 is G or is T, and is composed of 10 to 100 consecutive bases including the 36th base A polynucleotide or a polynucleotide complementary thereto; the base sequence of SEQ ID NO: 22 The 36th base of the polynucleotide is T or C, and the 36th base A polynucleotide or a polynucleotide corresponding thereto, which is composed of 10 to 100 consecutive bases including Complementary polynucleotide: 3 of the polynucleotide consisting of the base sequence of SEQ ID NO: 23 The 6th base is C or A, and 10 to 100 consecutive bases including the 36th base are a polynucleotide consisting of the bases of SEQ ID NO: No.: The 36th base of a polynucleotide consisting of a 24-base sequence is A or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; or a polynucleotide complementary thereto; consisting of the base sequence of SEQ ID NO: 25 The 36th base of the polynucleotide is T or C, and 10 A polynucleotide consisting of 100 to 100 consecutive bases or a polynucleotide complementary thereto Nucleotide; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 26 The base is T or C, and the base is 10 to 100 consecutive bases including the 36th base. A polynucleotide constructed as above or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence is T or C, A polynucleotide consisting of 10 to 100 consecutive bases including the base a polynucleotide complementary thereto; a polynucleotide consisting of the base sequence of SEQ ID NO: 28 the 36th base of the nucleotide is C or T, A polynucleotide consisting of 00 consecutive bases or a complementary polynucleotide, a sequence The 36th base of the polynucleotide consisting of the base sequence of sequence number 29 is A or G, A polynucleotide consisting of 10 to 100 consecutive bases including the 36th base or a polynucleotide complementary thereto; and a polynucleotide consisting of the base sequence of SEQ ID NO: 30 the 36th base of the nucleotide is T or G, and A polynucleotide consisting of 00 consecutive bases or a polynucleotide complementary thereto A SNP matrix for predicting high water temperature tolerance in Japanese flounder, including one or more species selected from the group consisting of A coating composition is provided.

[0020] The term "high water temperature resistance" in this disclosure means the property of being able to withstand an increase in water temperature, specifically 29.0°C. If the water temperature remains high, up to 33.7℃, the fish may suffer from temperature shock, weakened immune system, and decreased physiological functions. This means that the plant is resistant to growth retardation or disease outbreaks caused by factors such as:

[0021] The term "SNP marker" in this disclosure refers to a DNA sequence used to identify an individual or a species. A single nucleotide polymorphism refers to an allele base pair on the sequence. SNPs occur relatively frequently. It is stable and distributed throughout the genome, which generates genetic diversity among individuals, so S NP markers can serve as indicators of genetic proximity between individuals. NP markers generally involve phenotypic changes associated with single nucleotide polymorphisms, but in some cases In the case of the SNP markers of the present disclosure, variations in the amino acid sequence or This can reveal differences in individual phenotypes, such as tolerance to high water temperatures.

[0022] The term "individual" in this disclosure refers to a flounder (or flounder) that is the subject of the high water temperature tolerance test. The genotype of the SNP marker is determined by using a sample obtained from the flounder. By analyzing this, it is possible to identify flounder that can tolerate high water temperatures.

[0023] According to one embodiment of the present disclosure, in order to select SNPs predicting high water temperature resistance, After searching for SNPs in the parental genes through whole genome re-sequencing, Using the 70KSNP chips, we examined the high water temperature exposure of selected individuals. The search was narrowed to SNPs associated with temperature tolerance. We discovered an effective SNP in the body that is highly associated with high water temperature tolerance. The phenotype distribution was analyzed to determine the information of 30 SNPs, and the reliability and We confirmed that the accuracy of the method can predict the high water temperature tolerance of flounder.

[0024] Therefore, 10 to 100 consecutive bases containing the base sequence of SEQ ID NO: 1 of the present disclosure A polynucleotide consisting of the base or a complementary polynucleotide thereof or SEQ ID NO: 30 A polynucleotide consisting of 10 to 100 consecutive bases containing the base sequence The flounder containing one or more polynucleotides selected from the group consisting of: This suggests that the strain may be resistant to high water temperatures. So it was discovered for the first time.

[0025] Preferably, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 1 is A; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 2 is When the group is A, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 3 When the base is T, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 4 When the base is A, the base at position 36 of the polynucleotide consisting of the base sequence of SEQ ID NO: 5 is When the 36th base is G, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 6 When the 3rd base of the polynucleotide consisting of the base sequence of SEQ ID NO: 7 is C, When the sixth base is T, the polynucleotide consisting of the base sequence of SEQ ID NO: 8 When the 36th base is A: a polynucleotide consisting of the base sequence of SEQ ID NO: 9 wherein the 36th base is C; When the 36th base of the nucleic acid is A, the nucleic acid sequence of SEQ ID NO: 11 is When the 36th base of the polypeptide is T, the polypeptide having the base sequence of SEQ ID NO: 12 When the 36th base of the nucleotide is T, the nucleotide sequence of SEQ ID NO: 13 is When the 36th base of the polynucleotide is A, the base sequence of SEQ ID NO: 14 the 36th base of the polynucleotide having the base sequence of SEQ ID NO: 15 is C; the 36th base of the polynucleotide consisting of SEQ ID NO: 16 is G; When the 36th base of the polynucleotide consisting of the sequence of SEQ ID NO: 17 is A, When the 36th base of the polynucleotide consisting of the base sequence is A, the polynucleotide having the base sequence of SEQ ID NO: 1 8 base sequence, the 36th base of the polynucleotide is C; : When the 36th base of the polynucleotide consisting of the 19-base sequence is A; Number: When the 36th base of a polynucleotide consisting of a 20-base sequence is A; When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 21 is T; When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 22 is T, If the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 23 is A, When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 24 is A In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 25 is is C; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 26 is When the base is T, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 27 When the third base is T, the third base of the polynucleotide consisting of the base sequence of SEQ ID NO: 28 is When the sixth base is C, the polynucleotide consisting of the base sequence of SEQ ID NO: 29 or the 36th base of the base sequence of SEQ ID NO: 30. If the 36th base of the polynucleotide is T, it can be predicted that the flounder is high water temperature resistant. Cut.

[0026] From another viewpoint, the present disclosure provides a method for detecting or detecting the SNP marker for predicting high water temperature tolerance of flounder. A composition for predicting high water temperature tolerance in flounder is provided, which includes an amplifiable formulation.

[0027] The term "preparation capable of detecting or amplifying SNP markers" in the present disclosure refers to a genetic A composition that can predict high water temperature tolerant flounder by detecting or amplifying the polymorphic sites in offspring and preferably specifically detects or amplifies the polynucleotide of the SNP marker. The term "primer set" refers to a primer set or probe that can be used to identify a target gene.

[0028] The primers used for amplifying the SNP markers are prepared in a suitable buffer under suitable conditions ( For example, four different nucleoside triphosphates and DNA and RNA polymerases a polymerizing agent such as a reverse transcriptase or a template-directed DNA synthesis site under appropriate temperature The primer may be a single-stranded oligonucleotide that can act as a The appropriate length of the primer may vary depending on the intended use, but is usually 15 to 30 polynucleotides. Short primer molecules generally form stable hybrids with the template. The primer sequences are perfectly matched with the SNP markers. It does not have to be complementary, but is sufficiently complementary to hybridize with the SNP marker. It must be.

[0029] The term "primer" in this disclosure refers to a short, free 3'-terminal hydroxyl group. A base pair is formed with a complementary template. It refers to a short sequence that can be used as a starting point for copying a template strand, and is primarily a specific The primers are used in the form of a primer set that amplifies a region of interest. The primers are stored in an appropriate buffer solution and Reagents and temperature for polymerization reactions (i.e., DNA polymerase or reverse transcriptase) It can initiate DNA synthesis in the presence of four different nucleoside triphosphates. In this case, the PCR conditions and the lengths of the sense and antisense primers can be determined by methods known in the art. It can be modified based on what has been learned.

[0030] As used herein, the term "probe" refers to a DNA or RNA sequence of any length that is radioactively labeled. Alternatively, it is labeled with a fluorescent label or the like, and is composed of a single-stranded base sequence and has a complementary sequence. It is characterized by its ability to specifically bind and hybridize to single-stranded DNA or RNA with The target is detected by detecting the labeled signal of the hybridized probe. It is possible.

[0031] The primers or probes of the present disclosure may be prepared by phosphoramidite solid support methods or the like. Such nucleic acid sequences can be chemically synthesized using other widely known methods. The array can also be modified using many means known in the art. Non-limiting examples of such modifications include methylation, capping, substitution of one or more natural nucleotides, and the like. Nucleotide substitutions, such as uncharged linkers (e.g., methylphosphonates, phosphotriesters, phosphoramidates, carbamates, etc.) or charged linkers (e.g., phosphorothioates, phosphorodithioates, etc.) There is.

[0032] From another perspective, the present disclosure provides a method for predicting high water temperature tolerance of flounder, comprising the composition for predicting high water temperature tolerance of flounder. We provide a water temperature tolerance prediction kit.

[0033] The kits of the present disclosure include, but are not limited to, DNA chip kits. The DNA chip kit contains SNP markers, which are markers for predicting high water temperature tolerance in flounder. An oligonucleotide containing the sequence of a polynucleotide or its complementary base sequence is attached. The chip is used to specifically hybridize and bind to the target DNA. The feature is that the hybridization level changes depending on the base mutation of SNP. Specifically, the high water temperature tolerant flounder provided by the present disclosure can be predicted. The DNA chip kit for predicting water temperature tolerance contains a polynucleotide containing the base sequence of SEQ ID NO: 1. or a polynucleotide comprising the base sequence of SEQ ID NO: 30. or 10 to 10 polynucleotides containing the 36th base thereof The oligonucleotide complementary to the polynucleotide consisting of 0 bases is used as a marker. The target DNA is then reacted with the chip to determine the hybridization level. This includes a kit for predicting high water temperature tolerance in flounder, which determines the genotype based on the results.

[0034] From another perspective, the present disclosure relates to (a) isolating the high water temperature tolerance prediction S of paragraph 1 from the DNA of a flounder sample. (b) amplifying or detecting the polymorphic site of the NP marker; and or a method for predicting high water temperature tolerance of Japanese flounder, comprising a step of determining the base of the detected polymorphic site. to provide.

[0035] The step of amplifying polynucleotides from a DNA sample in step (a) can be carried out by a method known to those skilled in the art. For example, the target nucleic acid can be amplified via PCR and then It can be purified and obtained. Other applications include ligase chain reaction (LCR), transcription amplification (transcriptase Self-sustained sequence replication and nucleic acid-based sequence amplification (NASA) are used. It may be used.

[0036] The step (a) uses a primer or a probe to amplify or detect a base. It is possible.

[0037] Among the above methods, the method of determining the base at the amplified SNP marker site in step (b) The results are based on sequencing analysis, microarray hybridization, and allelic genetic analysis. Allele-specific PCR, dynamic allele hybridization dynamic allele-specific hybridization (DASH), PCR extension analysis, PCR- SSCP, PCR-RFLP analysis, HRM analysis or TaqMAN technique, SNPLex process platform (Applied Biosystems), mass spectrometry (e.g., Sequenom's MassARRAY system), system), mini-sequencing method, Bio-Plex system (B ioRad), CEQ and SNPstream system (Beckman), Molecular I Inversion Probe array technology (e.g., Affymetrix GeneChip), and Bea dArray technologies (e.g., Illumina GoldenGate and Infinium The method can be carried out by using, but is not limited to, the above-mentioned method. The SNP markers can be isolated by any of the methods described above or other methods available to those skilled in the art to which this disclosure pertains. One or more alleles can be identified in the car.

[0038] MODE FOR CARRYING OUT THE INVENTION Preferred examples are provided below to facilitate a better understanding of the present disclosure. The following examples are provided so that the present disclosure may be more readily understood. The content is not limited.

[0039] Example 1: High-density SNP 70K chip for flounder 1-1. Selection of mothers for analysis and securing high-density SNPs Eleven microsatellite markers (M S) Individuals selected based on the allele type of loci and the results of association analysis are classified as g Considering the QC results for the genomic DNA, population, mating availability, sex, etc., 100 fish were ultimately selected from the flounder population for genome resequencing analysis. Selected.

[0040] Genetic analysis of the 100 selected flounders was carried out using Illumina NovaSeq. The full-length genome sequence of Japanese flounder published in China was used as a reference sequence for analysis. As shown in Figure 1, trimming, read mapping, and sequencing After sorting and other steps, approximately 36.69 Gb of sequences were obtained for each sample. We have constructed a single-nucleotide polymorphism database and extracted approximately 80% of the individual-specific large-volume mutation information. We confirmed the nucleotide polymorphism (SNP). The ble sequence was subjected to four filtering methods to select high-quality SNPs. Then, candidate SNPs for constructing SNP chips were again selected.

[0041] 1-2. Final SNP selection and SNP chip production To fabricate high-density SNP chips, AXIOM TM myDesign n TM The SNP chip (50 to 90K) platform was selected. As shown in Figure 2, The high-quality SNPs obtained were divided into tiers according to their importance: 1) 100 genotype rate for LD block, LD repeat, and LD repeat. After ranking by MAF range, 154,964 SNPs were obtained. When analyzing the population structure of 100 flounders based on P, the 11 MS The results showed a similar pattern to the marker analysis, which indicates that the collected flounder population It is assumed that there is strong regional (population) specificity, and that genetic stability within populations is high. 55KSNPs from homopolymer regions and S with allele types A / T or C / G After removing NP, the SN was selected from the 88,065 recommendations. Considering the P-chip (50-90K) platform, 71,364 SNPs were finally identified. The selected SNPs were evenly distributed across the 24 chromosomes, and the distance between the SNPs was 70% were found to be less than 5,000 bp, with the majority being less than 10,000 bp. The chip was designed to accommodate 71,364 SNPs, for a total of 384 wells / pl We produced a 70KSNP tip for flounder in ate form.

[0042] Example 2: High water temperature exposure experiment on flounder 2-1. Preparation of experimental fish The flounder used as the experimental fish was collected from the flounder population at the Jeju Special Self-Governing Province Maritime and Fisheries Research Institute. The flounder population was produced in accordance with the Jeju National University breeding guidelines. 354 potential mothers (126 females and 288 males) were bred through natural spawning and natural fertilization. The F0 generation was mass-produced and reared to a size of 20-30 cm, and 800 of these were randomly selected. They were selected and placed in two 2.5m diameter tanks, 400 per tank, at a water temperature of 19.4°C. The fish were allowed to acclimate for three days. During this time, the tank used a circulating filtration system that could regulate the water temperature with a boiler. Individuals showing abnormal symptoms during the acclimation period were removed, and the individuals used in the high water temperature exposure experiment were The number of fish was 769. Feed was given twice a day until the water temperature reached 30°C, and feeding was stopped after the first death. did.

[0043] The water temperature was adjusted so that it could rise from the acclimation temperature to 30°C by about 1°C per day, and the critical temperature for flounder was reached. The temperature was adjusted to rise by 0.5°C per day up to 32.5°C, which is known as the temperature. Water temperature and dissolved oxygen were measured at regular intervals to ensure that oxygen saturation was maintained above 90%. From the time of the first death, dead individuals were collected at regular intervals and their size, weight, and external appearance were measured. Symptoms were confirmed, and the tail fins were collected and frozen for storage.

[0044] 2-2. Microarray for comparing high water temperature tolerance Genomic DNA (gDNA) was extracted from 50 mg of the tail fin of the experimental fish using QIAamp96DN. Extraction was performed using an AQIAcubeHT Kit (Qiagen, Germany). We performed gDNA QC (quality control) using a 70KSNP chip designed and manufactured by us. Microarray analysis was performed on the 763 samples that passed.

[0045] Microarray raw data was collected using AxiomAnalysisSuite5. 726 SNPs that passed sample QC were analyzed using the .1.1 software (Thermo Fisher). C was analyzed and high-resolution polymorphic data of 59,128 SNPs were extracted.

[0046] Example 3: Statistical Analysis 3-1. Phenotype data analysis As shown in Table 1, the average total length of the 769 experimental fish was 25.42 ± 1 .63cm, average body width was 8.84±0.65cm, and average body weight was 1 The calculated weight was 59.1±29.9g.

[0047] [Table 1]

[0048] Referring to Figure 1, the water temperature began to rise, and the water temperature at which the first deaths occurred was 30.7°C. For four days after the first death, the water temperature rose from 30.7°C to a maximum of 32.7°C and remained there. After death, the water temperature was lowered from the early morning of the fourth day, and the fish that survived were classified as survivors. The number of dead fish was 538, with a cumulative mortality rate of 69.96%, and 231 surviving fish were confirmed. (Table 2).

[0049] [Table 2]

[0050] The survival analysis of high water temperature exposure was carried out based on 1) whether or not there was survival, and 2) how many times the first death occurred. 3) DPC_Day, which is the number of days since the first death. The survival time (DPC_Time) was divided into points and assigned to the analysis.

[0051] 3-2. Genome-wide association study (GWAS) The genotype data of each experimental individual was quality controlled using PLINK1.9 software. Proceed with QC and save the SNP values ​​in binary format in bfile format for the next analysis. After that, we used the R package Gaston to analyze linear mixed models. We estimate SNP communities based on the LMM (Likely a mixture model) and perform binary and quantitative e) Analysis of traits was carried out.

[0052] The proportion of phenotypic variation was estimated using the following formula: It was determined.

[0053]

number

[0054] Genome-Wide Association Study (GWAS) based on survival In the above, surviving individuals are assigned a value of 1 and dead individuals are assigned a value of 0, and the significance level is calculated using the Bonferroni correction method. Using 0.05 / 58,920=8.47x10 -7 Table 3 and Figure 2a For reference, the heritability of survival (h 2 ) is estimated to be 0.620, which Specific SNPs associated with HIV-1 were identified on chromosomes 18 and 19.

[0055] [Table 3] JPEG2025188108000006.jpg255168

[0056] GWAS analysis based on survival days (DPC_Day) was performed by assigning a value of 1 to 8 for each individual. The significance level was set at 0.05 / 58,920 = 8.47x10 using the Peroni correction method. -7 Based on Table 4 and Figure 2b, the heritability of DPC_Day was 0. 753 were estimated, and the specific SNPs associated with these were 18, similar to the survival GWAS results. and chromosome 19.

[0057] [Table 4] JPEG2025188108000008.jpg255167

[0058] For GWAS analysis based on survival time (DPC_Time), the death date of each individual was calculated using Excel. After converting it into a serial number using The Bonferroni correction method was used to estimate the mean value of 0.05 / 58,920 = 8.47 x 10 -7 in Referring to Table 5 and Figure 2C, the heritability by survival time was estimated to be 0.710. The specific SNPs associated with this were chromosomes 18 and 19, similar to the survival GWAS results. confirmed by color body.

[0059] [Table 5] JPEG2025188108000010.jpg255170

[0060] 3-3. SNP screening for predicting high water temperature tolerance The main S confirmed through GWAS analysis according to the survival, survival days, and survival time Based on the NP information, 30 SNPs were selected to predict high water temperature resistant individuals. The base sequence and information on the gene associated with each SNP are shown in Table 6.

[0061] [Table 6] JPEG2025188108000012.jpg255170JPEG2025188108000013.jpg255170JPEG2025188108000014.jpg255170JPEG2025188108000015.jpg135170

[0062] Based on the following analysis results, the 30 SNPs were determined to be high water temperature tolerant genotypes. Information on each SNP associated with temperature tolerance and mean and standard survival times and survival days by genotype The deviations and graphs are shown in Table 7 and Figures 3 to 32 below. The survival days (DPC_Day) are the survival times for each individual. Specify a value between 1 and 8 for the number of days, and use Excel to calculate the survival time (DPC_Time) by calculating the date of death for each individual. After converting it into a serial number using the above, the first dead individual occurrence date was set as 1. The survival status of individuals and genotypes are correlated and analyzed to identify individuals who survived or died for each genotype. The numbers were checked and used to plot the bar graph.

[0063] [Table 7] JPEG2025188108000017.jpg253170JPEG2025188108000018.jpg251170JPEG2025188108 000019.jpg253170JPEG2025188108000020.jpg249170JPEG2025188108000021.jpg68170

[0064] Referring to Table 7 and Figures 3 to 32, when high water temperature tolerant genotypes are included, DPC It was confirmed that the average was high and the number of surviving individuals was high. When selecting individuals with a sexual genotype, it can be expected that they will have a higher survival probability at high water temperatures.

[0065] 3-4. Heritability analysis of high water temperature tolerance GWAS analysis of high water temperature tolerance through genetic parameter analysis The heritability estimates are shown in Table 8.

[0066] [Table 8]

[0067] In survival, survival days, and survival time-based analyses, heritability is approximately 0.620-0.753. Therefore, it is expected that the relevant traits can be improved through genetic selection. It is expected that SNP markers can be used as markers for selecting individuals tolerant to high water temperatures. do. [Industrial Applicability]

[0068] SNP (Single nucleotide polymorphism) marker composition for predicting high water temperature tolerance according to the present disclosure This material can lead to the development of high water temperature tolerant varieties and sustainable production, and is of great value to flounder farming. It can be very helpful.

Claims

1. The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 1 is G or A. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 2 is A or G. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 3 is T or C and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 4 is A or G. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 5 is T or G. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 6 is C or A. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 7 is T or G. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 8 is A or G and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 9 is T or C. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 10 is G or A polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 11 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 12 is T or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 13 is A or G. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 14 is C or T, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 15 is A or G. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 16 is A or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 17 is A or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 18 is T or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 19 is A or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 20 is G or A polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 21 is G or T. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 22 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 23 is C or A. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 24 is A or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 25 is T or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 26 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 27 is T or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 28 is C or T. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 29 is A or G, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base a nucleotide or a polynucleotide complementary thereto; and The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 30 is T or G. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; Contains one or more selected from the group consisting of SNP (Single nucleotide polymerase chain reaction) for predicting high water temperature tolerance in Japanese flounder orphism) marker composition.

2. When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 1 is A, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 2 is A In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 3 is T, When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 4 is In the case of A, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 5 is G; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 6 is When the group is C, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 7 When the base is T, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 8 is A; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 9 When the third base is C, the third base of the polynucleotide consisting of the base sequence of SEQ ID NO: 10 is When the sixth base is A, the polynucleotide has the base sequence of SEQ ID NO:

11. wherein the 36th base is T; When the 36th base of the nucleic acid sequence is T, the nucleic acid sequence of the nucleic acid sequence of SEQ ID NO: 13 is When the 36th base of the polypeptide is A, the polypeptide having the base sequence of SEQ ID NO: 14 When the 36th base of the nucleotide is C, the nucleotide sequence of SEQ ID NO: 15 is When the 36th base of the polynucleotide is G, the base sequence of SEQ ID NO: 16 the 36th base of the polynucleotide is A; In the case where the 36th base of the polynucleotide consisting of SEQ ID NO: 18 is A; When the 36th base of the polynucleotide consisting of the sequence of SEQ ID NO: 19 is C, When the 36th base of the polynucleotide consisting of the base sequence is A, the polynucleotide of SEQ ID NO: 2 0, the 36th base of the polynucleotide having the base sequence of SEQ ID NO: 2 When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 1 is T, When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 2 is T, 3, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: : When the 36th base of a polynucleotide consisting of a 24-base sequence is A; Code: When the 36th base of a polynucleotide consisting of a 25-base sequence is C; Sequence number: When the 36th base of a polynucleotide consisting of a 26-base sequence is T; When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 27 is T the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 28 is C; In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 29 is G or the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 30 If the base is T, it is predicted that the flounder is high water temperature resistant. The SNP marker composition for predicting high water temperature tolerance of flounder according to claim 1.

3. A preparation capable of detecting or amplifying SNP markers for predicting high water temperature tolerance in flounder. A composition for predicting high water temperature resistance, The SNP marker is The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 1 is G or A. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 2 is A or G and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 3 is T or C. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 4 is A or G and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 5 is T or G. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 6 is C or A and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 7 is T or G. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 8 is A or G and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 9 is T or C. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 10 is G or A polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 11 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 12 is T or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 13 is A or G. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 14 is C or T, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 15 is A or G. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 16 is A or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 17 is A or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 18 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 19 is A or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 20 is G or A polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 21 is G or T. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 22 is T or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 23 is C or A. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 24 is A or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 25 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 26 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 27 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 28 is C or T. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 29 is A or G. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; a nucleotide or a polynucleotide complementary thereto; and The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 30 is T or G. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; Contains one or more selected from the group consisting of Composition for predicting tolerance of flounder to high water temperatures.

4. The formulation may contain primers or probes capable of detecting or amplifying the SNP markers. The composition for predicting high water temperature resistance of flounder according to claim 3, characterized in that

5. A kit for predicting high water temperature tolerance of flounder, comprising the composition according to claim 3.

6. (a) SNP markers for predicting high water temperature tolerance in flounder from DNA samples isolated from flounder amplifying or detecting the polymorphic site of (b) determining the base of the polymorphic site amplified or detected in step (a), The SNP marker in step (a) is The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 1 is G or A. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 2 is A or G and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 3 is T or C. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 4 is A or G and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 5 is T or G. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 6 is C or A and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 7 is T or G. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 8 is A or G and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 9 is T or C. and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 10 is G or A polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 11 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 12 is T or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 13 is A or G. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 14 is C or T, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 15 is A or G. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 16 is A or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 17 is A or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 18 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 19 is A or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 20 is G or A. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 21 is G or T, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 22 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 23 is C or A. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 24 is A or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 25 is T or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 26 is T or C. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 27 is T or C, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base. a nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 28 is C or T. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 29 is A or G, and a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base a nucleotide or a polynucleotide complementary thereto; and The 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 30 is T or G. a polynucleotide consisting of 10 to 100 consecutive bases including the 36th base; nucleotide or a polynucleotide complementary thereto; Contains one or more selected from the group consisting of A method for predicting the tolerance of flounder to high water temperatures.

7. When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 1 is A, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 2 is A In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 3 is T, In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 4 is A, In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 5 is G When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 6 is In the case of C, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 7 is T; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 8 is When the group is A, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 9 When the base is C, the base is the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO:

10. When the third base is A, the third base of the polynucleotide consisting of the base sequence of SEQ ID NO: 11 is When the sixth base is T, the polynucleotide has the base sequence of SEQ ID NO:

12. wherein the 36th base is T; When the 36th base of the nucleic acid is A, the nucleic acid sequence of the nucleic acid sequence of SEQ ID NO: 14 is When the 36th base of the polypeptide is C, the polypeptide having the base sequence of SEQ ID NO: 15 When the 36th base of the nucleotide is G, the nucleotide sequence of SEQ ID NO: 16 is When the 36th base of the polynucleotide is A, from the base sequence of SEQ ID NO: 17 the 36th base of the polynucleotide is A; The 36th base of the polynucleotide consisting of SEQ ID NO: 19 is C; When the 36th base of the polynucleotide consisting of the sequence of SEQ ID NO: 20 is A, When the 36th base of the polynucleotide consisting of the base sequence is A, the polynucleotide of SEQ ID NO: 2 In the case where the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: : When the 36th base of a polynucleotide consisting of a 22-base sequence is T; Number: When the 36th base of a polynucleotide consisting of a 23-base sequence is A; When the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 24 is A, the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 25 is C; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 26 is T the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 27; is T; the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO: 28 is When the base is C, the base is the 36th base of the polynucleotide consisting of the base sequence of SEQ ID NO:

29. or a polynucleotide consisting of the base sequence of SEQ ID NO:

30. If the 36th base of the sequence is T, it is predicted that the flounder is high water temperature resistant. The method for predicting high water temperature resistance of flounder according to claim 6.

Images