Anti-aging composition containing uridylic acid, adenylic acid and yeast peptides and use thereof

Abstract

The present invention discloses an anti-aging composition containing uridylic acid, adenylic acid and yeast peptide and its use, which belongs to the medical and health technical field. The composition is made of 5'-uridylic acid, 5'-adenylic acid, yeast peptide and functional active ingredients in a certain ratio, and the functional active ingredients include one or more of antler extract, pyrroloquinoline quinone, glutathione, curcumin, tea polyphenol, quercetin, resveratrol, α-lipoic acid, α-ketoglutaric acid and fisetin. The composition containing uridylic acid, adenylic acid and yeast peptide plays a role of delaying aging and restoring vitality to the human body, and has the effects of delaying, improving and preventing aging.

Description

[Technical field]

[0001] The present invention relates to the field of medicine and health care, and in particular to an anti-aging composition containing uridylic acid, adenylic acid and yeast peptide and its use. [Background technology]

[0002] Aging is a necessary stage in the life cycle and has always accompanied the development of human civilization. In the 21st century, humans have conducted more detailed research into aging, and people's understanding of aging has gradually changed. Currently, anything that is beneficial to human health and improves the quality of life can be defined as anti-aging (anti-aging). The main methods of slowing down aging are both non-drug and drug-based, of which non-drug methods include living a scientifically-regulated life and maintaining good lifestyle habits. There are many drug-based methods, most of which involve taking health drugs and health foods, and their effects are also the most remarkable.

[0003] Currently, anti-aging products on the market use industrial ingredients with strong antioxidant properties to achieve excellent anti-aging effects, which work quickly in the short term, but are extremely harmful to the human body when taken for a long time. Some anti-aging products contain vitamin antioxidants, but these products are not stable.

[0004] Nucleotides function as precursors for synthesizing nucleic acids in the body. As basic building blocks of ribonucleic acid and deoxyribonucleic acid, they are distributed in the cell nuclei and cytoplasm of various organs and tissues in the body along with nucleic acids, and as building blocks of nucleic acids, they are involved in basic life activities such as genetics, development, and growth of living organisms. Summary of the Invention [Problem to be solved by the invention]

[0005] The present invention aims to provide an anti-aging composition containing uridylic acid, adenylic acid and yeast peptide and its use. Through long-term research, the present invention has found that different nucleotides have various functions, and after adding other functional active ingredients (especially yeast peptide), it can effectively remove free radicals in the body, delay cell aging, and restore vitality to the human body. The present invention provides a new method for the use of nucleotide nutritional products in human health level and people's quality of life. [Means for solving the problem]

[0006] The present invention achieves the above object by the following technical means. In one aspect of the present invention, there is provided an anti-aging composition comprising uridylic acid, adenylic acid and yeast peptides, the anti-aging composition comprising 5'-uridylic acid, 5'-adenylic acid, yeast peptides and functional active ingredients comprising one or more of antler extract, pyrroloquinoline quinone (PQQ), glutathione, curcumin, tea polyphenols, quercetin, resveratrol, alpha-lipoic acid, alpha-ketoglutaric acid and fisetin.

[0007] Additionally, the composition contains, by weight, 0.3-1.2 parts 5'-uridylic acid, 0.3-1.2 parts 5'-adenylic acid, 0.05-1.5 parts yeast peptides, and 0.88-7.35 parts functional active ingredient.

[0008] Further, the functional active ingredients include, by weight, one or more of 1-2 parts deer antler extract, 0.01-0.05 parts pyrroloquinoline quinone (PQQ), 0.25-1 parts glutathione, 0.12-0.6 parts curcumin, 0.5-1 parts tea polyphenols, 0.5-1.25 parts quercetin, 0.02-0.1 parts resveratrol, 0.01-0.1 parts alpha-lipoic acid, 0.3-1 parts alpha-ketoglutaric acid, and 0.05-0.25 parts fisetin.

[0009] The composition further comprises, by weight, 0.6 parts of 5'-uridylic acid, 0.6 parts of 5'-adenylic acid, 0.25 parts of yeast peptide, 2 parts of antler extract, 0.02 parts of pyrroloquinoline quinone, 0.25 parts of glutathione, 0.19 parts of curcumin, 0.5 parts of tea polyphenols, 0.5 parts of quercetin, and 0.051 parts of resveratrol; or The composition contains, by weight, 0.6 parts 5'-uridylic acid, 0.6 parts 5'-adenylic acid, 0.25 parts yeast peptide, 2 parts antler extract, 0.05 parts alpha-lipoic acid, 0.3 parts alpha-ketoglutaric acid, 0.02 parts pyrroloquinoline quinone, 0.19 parts curcumin, 0.1 parts fisetin, 0.25 parts glutathione, and 0.051 parts resveratrol.

[0010] The composition further comprises, by weight, 0.3 parts 5'-uridylic acid, 0.3 parts 5'-adenylic acid, 0.17 parts yeast peptide, 0.01 parts pyrroloquinoline quinone, 0.25 parts glutathione, 0.12 parts curcumin, and 0.5 parts tea polyphenols; 0.6 parts 5'-uridylic acid, 0.6 parts 5'-adenylic acid, 0.25 parts yeast peptide, 0.02 parts pyrroloquinoline quinone, 0.25 parts glutathione, 0.19 parts curcumin, 0.5 parts tea polyphenols, 0.5 parts quercetin, and 0.051 parts resveratrol; or It contains 0.3 parts of lysyl acid, 0.3 parts of 5'-adenylic acid, 0.17 parts of yeast peptide, 0.05 parts of fisetin, 0.01 parts of pyrroloquinoline quinone, 0.25 parts of glutathione, 0.12 parts of curcumin and 0.5 parts of tea polyphenols, or contains 0.6 parts of 5'-uridylic acid, 0.6 parts of 5'-adenylic acid, 0.25 parts of yeast peptide, 0.05 parts of α-lipoic acid, 0.3 parts of α-ketoglutaric acid, 0.02 parts of pyrroloquinoline quinone, 0.19 parts of curcumin, 0.1 parts of fisetin, 0.25 parts of glutathione and 0.051 parts of resveratrol.

[0011] Furthermore, the antler extract is derived from fresh antlers. The fresh antlers used in the present invention are preferably young antlers of male deer such as Cervus nippon Temminck or Cervus elaphus Linnaeus that are not ossified and have dense hair. The extraction method of the antler extract used in the present invention includes, but is not limited to, conventional decomposition methods in this field, organic solvent extraction methods, enzymatic decomposition, ultrasonic disruption, CO2 supercritical extraction, high-voltage pulsed electric field (PEF) method, etc. The main components of the antler extract used in the present invention are polypeptides, proteins, and polysaccharides. In the present invention, the antler extract can achieve the effects of the present invention as long as the content of polypeptides of 100 to 5000 Da reaches 40 wt% or more, the content of collagen and keratin reaches 10 wt% or more, and the content of polysaccharides reaches 30 wt% or more. There are no particular limitations on other components as long as the extract is extracted by a conventional method in this field.

[0012] The yeast peptide used in the present invention is a type of fermentation product of whole nutritional yeast, and its main components are short peptides (peptides consisting of 5 to 20 amino acids) of less than 500 to 2000 Da and various amino acids, and further contains polysaccharides, various vitamins, minerals, and dietary fiber to supplement various nutrients lacking in the aging human body. In the present invention, the yeast peptide can achieve the effects of the present invention when it satisfies the following: the content of short peptides (peptides consisting of 5 to 20 amino acids) of less than 500 to 2000 Da is 40 wt% or more, and the amino acids contained are essential amino acids / total amino acids are 40 wt% or more, and branched chain amino acids / total amino acids are 15 wt% or more. There are no particular limitations on other nutritional components such as polysaccharides, various vitamins, minerals, and dietary fiber, as long as they are fermentation products produced by fermenting whole nutritional yeast using a conventional method in this field. In the present invention, the yeast is preferably Saccharomyces cerevisiae, the fermentation substrate is preferably molasses including sugarcane molasses and beet molasses, and phosphoric acid, zinc sulfate, ammonium sulfate, magnesium sulfate, etc. are added as fermentation nutrients.

[0013] Furthermore, the finished composition may be in the form of a powder, granules, tablet, pill, oral liquid or gelatin.

[0014] The present invention further provides the use of the anti-aging composition containing the above-mentioned uridylic acid, adenylic acid and yeast peptide in the manufacture of anti-aging medicines, health care products or nutritional foods.

[0015] Moreover, said medicine, said health care product or said nutritional food can eliminate free radicals in the human body, resist oxidation, effectively protect and repair damaged cells, delay aging and play a vital role in restoring the human body. Effect of the Invention

[0016] The present invention has the following beneficial effects compared to the prior art: The present invention provides a composition containing 5'-uridylic acid, 5'-adenylic acid, yeast peptides and other functional active ingredients, and the use thereof. The functional active ingredients include one or more of antler extract, pyrroloquinoline quinone (PQQ), glutathione, curcumin, tea polyphenols, quercetin, resveratrol, α-lipoic acid, α-ketoglutaric acid and fisetin. Research has shown that 5'-uridylic acid and 5'-adenylic acid can improve the survival rate of probiotics in the gastrointestinal tract, improve the intestinal environment, promote the decomposition of macromolecular substances, and accelerate the absorption and transport of various active ingredients. And, it has been verified through speculation and experimental results that the aging delay effect and anti-aging effect of the composition can be enhanced by combining nucleotides and yeast peptides with other active ingredients. [Brief description of the drawings]

[0017] [Figure 1] 1 shows the effect of the composition containing uridylic acid, adenylic acid and yeast peptide of the present invention on the content of glutathione peroxidase (GSH-PX) in the serum of D-galactose mice, where # indicates that the difference (P<0.05) is statistically significant compared to the model group, and * indicates that the difference (P<0.05) is statistically significant compared to the blank group. [Diagram 2] 2 shows the effect of the composition containing uridylic acid, adenylic acid and yeast peptide of the present invention on the content of superoxide dismutase (SOD) in the serum of D-galactose mice, where # indicates that the difference (P<0.05) is statistically significant compared to the model group, and * indicates that the difference (P<0.05) is statistically significant compared to the blank group. [Diagram 3] 3 shows the effect of the composition containing uridylic acid, adenylic acid and yeast peptide of the present invention on the content of malondialdehyde (MDA) in serum of D-galactose mice, where # indicates that the difference is statistically significant (P<0.05) compared to the model group. [Figure 4] FIG. 4 shows the effect of a composition containing uridylic acid, adenylic acid and yeast peptides of the present invention on the lifespan of aged mice. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0018] The present invention will be further described below in conjunction with specific examples, but it should be understood that these examples are merely illustrative and do not limit the scope of the present invention in any manner. In the following examples, unless otherwise specified, all experimental methods used are conventional, and materials, reagents, etc. used are purchased from bio- or chemical companies.

[0019] Example 1 In a clean place, 0.6 parts by weight of 5'-uridylic acid, 0.6 parts by weight of 5'-adenylic acid, 0.25 parts by weight of yeast peptide, 2 parts by weight of antler extract, 0.02 parts by weight of pyrroloquinoline quinone, 0.25 parts by weight of glutathione, 0.19 parts by weight of curcumin, 0.5 parts by weight of tea polyphenol, 0.5 parts by weight of quercetin, and 0.051 parts by weight of resveratrol are weighed out and mixed uniformly in a mixer to obtain a powder formulation. The powder formulation (finished product) is sampled and inspected for various indicators according to the quality standard regulations, and is stored and prepared after passing the inspection.

[0020] Example 2 In a clean place, 0.6 parts by weight of 5'-uridylic acid, 0.6 parts by weight of 5'-adenylic acid, 0.25 parts by weight of yeast peptide, 2 parts by weight of antler extract, 0.05 parts by weight of α-lipoic acid, 0.3 parts by weight of α-ketoglutaric acid, 0.02 parts by weight of pyrroloquinoline quinone, 0.19 parts by weight of curcumin, 0.1 parts by weight of fisetin, 0.25 parts by weight of glutathione, and 0.051 parts by weight of resveratrol are weighed out and mixed uniformly in a mixer to obtain a powder formulation. The powder formulation (finished product) is sampled and inspected for various indicators according to the quality standard regulations, and is stored and prepared after passing the inspection.

[0021] Example 3 In a clean place, 0.3 parts of 5'-uridylic acid, 0.3 parts of 5'-adenylic acid, 0.17 parts of yeast peptide, 0.01 parts of pyrroloquinoline quinone, 0.25 parts of glutathione, 0.12 parts of curcumin, and 0.5 parts of tea polyphenol are weighed out by weight and mixed uniformly in a mixer to obtain a powder formulation. The powder formulation (finished product) is sampled and inspected for various indicators according to the quality standard regulations, and is stored for preparation after passing the inspection.

[0022] Example 4 In a clean place, 0.6 parts of 5'-uridylic acid, 0.6 parts of 5'-adenylic acid, 0.25 parts of yeast peptide, 0.02 parts of pyrroloquinoline quinone (PQQ), 0.25 parts of glutathione, 0.19 parts of curcumin, 0.5 parts of tea polyphenol, 0.5 parts of quercetin, and 0.051 parts of resveratrol are weighed out by weight and mixed uniformly in a mixer to obtain a powder formulation. The powder formulation (finished product) is sampled and inspected for various indicators according to the quality standard regulations, and is stored and prepared after passing the inspection.

[0023] Example 5 In a clean place, 0.3 parts of 5'-uridylic acid, 0.3 parts of 5'-adenylic acid, 0.17 parts of yeast peptide, 0.05 parts of fisetin, 0.01 parts of pyrroloquinoline quinone (PQQ), 0.25 parts of glutathione, 0.12 parts of curcumin, and 0.5 parts of tea polyphenol are weighed out by weight and mixed uniformly in a mixer to obtain a powder formulation. The powder formulation (finished product) is sampled and inspected for various indicators according to the quality standard regulations, and is stored and prepared after passing the inspection.

[0024] Example 6 In a clean place, 0.6 parts by weight of 5'-uridylic acid, 0.6 parts by weight of 5'-adenylic acid, 0.25 parts by weight of yeast peptide, 0.05 parts by weight of α-lipoic acid, 0.3 parts by weight of α-ketoglutaric acid, 0.02 parts by weight of pyrroloquinoline quinone, 0.19 parts by weight of curcumin, 0.1 parts by weight of fisetin, 0.25 parts by weight of glutathione, and 0.051 parts by weight of resveratrol are weighed out and mixed uniformly in a mixer to obtain a powder formulation. The powder formulation (finished product) is sampled and inspected for various indicators according to the quality standard regulations, and is stored and prepared after passing the inspection.

[0025] Example 7 Antioxidant performance of compositions containing uridylic acid, adenylic acid and yeast peptides The powder formulation prepared in Example 1 was weighed and dissolved in a mixture of deionized water and ethanol (volume ratio 1:1) to prepare a mother solution with a concentration of 1 mg / mL. - The free radical scavenging rate is measured.

[0026] 1. Measurement of DPPH free radical scavenging rate: Dilute the mother liquor 100x, 50x, 25x, 10x, and 1x to prepare serial dilutions with concentrations of 10μg / mL, 20μg / mL, 40μg / mL, 100μg / mL, and 500μg / mL.

[0027] 150μL of each sample of the above concentration 1 and 150μL of 60mg / L DPPH solution (diluted with absolute ethanol) are placed in a 96-well plate, mixed uniformly, stored in the dark at 30℃, and after 30 minutes, the absorbance value at 519nm is measured using a microplate reader. The calculation formula for the scavenging rate of DPPH free radicals is as follows:

number

[0028] 2.OH - Measurement of free radical scavenging rate: (1) Dilute the mother liquor 100x, 50x, 25x, 10x, and 1x to prepare serial dilutions of 10 μg / mL, 20 μg / mL, 40 μg / mL, 100 μg / mL, and 500 μg / mL. (2) Add 50 μL of each of the above concentrations of samples and 50 μL of 6 mmol / L FeSO4 solution to 50 μL of 6 mmol / L ethanol-salicylic acid solution, place this in a 96-well plate, mix uniformly, then add 50 μL of 6 mmol / L H2O2 solution and mix uniformly. (3) After reacting at 37°C for 30 minutes, the 96-well plate is placed in a microplate reader and the absorbance value at 510 nm is measured (A サンプル ).OH - The formula for calculating the free radical scavenging rate is as follows:

number

[0029] Referring to the above method, the DPPH free radical removal rate and OH radical removal rate of the powder preparation samples prepared in Examples 2-6 were - The rate of free radical scavenging is measured. Control group 1: single component 5′-uridylic acid. Control group 2: single component 5′-adenylic acid. Control group 3: single component yeast peptide. Control Group 4: A blended composition containing, by weight, 0.6 parts of 5'-uridylic acid and 0.6 parts of 5'-adenylic acid. Control Group 5: A compounded composition containing, by weight, 0.6 parts of 5'-uridylic acid, 0.6 parts of 5'-adenylic acid, and 0.25 parts of yeast peptide. Control Group 6: A blended composition containing, by weight, 0.6 parts 5'-guanylic acid, 0.6 parts 5'-cytidylic acid, and 0.25 parts yeast peptide. Control Group 7: A formulation composition containing, by weight, 0.25 parts yeast peptide, 2 parts antler extract, 0.02 parts pyrroloquinoline quinone (PQQ), 0.25 parts glutathione, 0.19 parts curcumin, 0.5 parts tea polyphenols, 0.5 parts quercetin, and 0.051 parts resveratrol. Control Group 8: A formulation composition containing, by weight, 0.25 parts yeast peptide, 0.02 parts pyrroloquinoline quinone (PQQ), 0.25 parts glutathione, 0.19 parts curcumin, 0.5 parts tea polyphenols, 0.5 parts quercetin, and 0.051 parts resveratrol.

[0030] The experimental data are expressed as mean values ​​± standard deviations, and the experimental results are shown in Table 1.

[0031] [Table 1]

[0032] According to the results, the compositions containing uridylic acid, adenylic acid and yeast peptides in Examples 1 to 6 had higher antioxidant performance than the controls 1 to 8, and reduced DPPH free radicals and OH -It was shown that the composition of Examples 1 to 6 can more effectively remove free radicals. It was also shown that the antioxidant performance was significantly improved after yeast peptides and functional ingredients were added to uridylic acid and adenylic acid. It was demonstrated that the compositions of Examples 1 to 6 can more effectively remove free radicals in the body.

[0033] The composition of the present invention, which is made by combining uridylic acid, adenylic acid, yeast peptides, and a functional ingredient, has a significantly greater ability to scavenge free radicals than any of a single component uridylic acid, a single component adenylic acid, a single component yeast peptide, a combination of yeast peptides and a functional ingredient alone, a combination of two nucleotides (5'-uridylic acid and 5'-adenylic acid in a 1:1 weight ratio), a combination of two nucleotides and yeast peptides (5'-uridylic acid, 5'-adenylic acid, and yeast peptide in a 0.6:0.6:0.25 weight ratio), or a combination of 5'-guanylic acid, 5'-cytidylic acid, and yeast peptide, and has a significantly greater ability to scavenge free radicals than any of a single component uridylic acid, a single component adenylic acid, a single component yeast peptide, a combination of yeast peptides and a functional ingredient alone, a combination of two nucleotides (5'-uridylic acid, 5'-adenylic acid, and yeast peptide in a 0.6:0.6:0.25 weight ratio), or a combination of 5'-guanylic acid, 5'-cytidylic acid, and yeast peptide. - The ability to scavenge free radicals has been greatly improved. In addition, it can enhance the activity of antioxidant enzymes in the body to achieve the purpose of delaying aging. Uridylic acid and adenylic acid work synergistically with yeast peptides to enhance the ability to scavenge free radicals, and the effect is further improved after the addition of functional ingredients.

[0034] Example 8 In vitro anti-aging retardation performance of a composition containing uridylic acid, adenylic acid and yeast peptides Using human umbilical vein endothelial cells and PC-12 cells (rat adrenal pheochromocytoma cells) as models, modeling is performed using H2O2, and an evaluation experiment on the intracellular ROS levels of the compositions of Examples 1 to 6 is performed. Control group 1: single component 5′-uridylic acid. Control group 2: single component 5′-adenylic acid. Control group 3: single component yeast peptide. Control Group 4: A blended composition blended with 0.6 parts of 5'-uridylic acid and 0.6 parts of 5'-adenylic acid, by weight. Control Group 5: A formulation composed of, by weight, 0.6 parts 5'-uridylic acid, 0.6 parts 5'-adenylic acid, and 0.25 parts yeast peptide. Control Group 6: A blended composition formulated with, by weight, 0.6 parts 5'-guanylic acid, 0.6 parts 5'-cytidylic acid, and 0.25 parts yeast peptide. Control Group 7: A formulation containing, by weight, 0.25 parts yeast peptide, 2 parts antler extract, 0.02 parts pyrroloquinoline quinone (PQQ), 0.25 parts glutathione, 0.19 parts curcumin, 0.5 parts tea polyphenols, 0.5 parts quercetin, and 0.051 parts resveratrol. Control Group 8: A formulation composition formulated with, by weight, 0.25 parts yeast peptide, 0.02 parts pyrroloquinoline quinone, 0.25 parts glutathione, 0.19 parts curcumin, 0.5 parts tea polyphenols, 0.5 parts quercetin, and 0.051 parts resveratrol.

[0035] The large amount of ROS generated by the action of hydrogen peroxide on cells attacks cell membranes, proteins, and nucleic acids, causing cell damage. Cells in the logarithmic growth phase were digested into a single cell suspension and the cell concentration was adjusted to 10 5The cells are adjusted to 100 μL / mL and inoculated into a 96-well plate with 6 parallel wells for each group. 24 hours after inoculation, the cells attach to the plate wall and grow. The supernatant is discarded, and a medium containing H2O2 at a final concentration of 200 μmol / L is added and cultured for 4 hours, after which the medium is replaced with the composition culture solution of Examples 1 to 6 at a final concentration of 50 μg / mL and cultured for 24 hours. At the same time, a blank control group to which no H2O2 is added and a model control group to which only H2O2 is added are set up, and the status of intracellular reactive oxygen is evaluated using a reactive oxygen species detection kit. 10 μmol / L of DCFH-DA mother solution (dissolved in DMSO) is added to each well to a final concentration of 1 μmol / L. After 2 hours of incubation, the supernatant was discarded and 100 μL of PBS was added to each well, washed twice, centrifuged, and then resuspended in 200 μL of PBS. The fluorescence intensity of each well was measured using a fluorescence microplate reader under the conditions of an excitation wavelength of 485 nm and an emission wavelength of 525 nm, and the cellular reactive oxygen content was calculated. The experimental data was expressed as the mean value ± standard deviation, and the experimental results are shown in Table 2.

[0036] [Table 2]

[0037] According to the above results, it was demonstrated that the compositions of Examples 1 to 6 significantly reduced reactive oxygen species in the model cells compared with the formulation compositions of the model group and the control groups 1 to 8, and had good protective and repairing effects on oxidatively damaged cells.

[0038] The composition of the present invention, which is produced by combining uridylic acid, adenylic acid, yeast peptide, and a functional ingredient, has the ability to significantly reduce the active oxygen content in cells damaged by H2O2, and is advantageous for repairing cells damaged by aging and oxidation, and significantly improves the ability to reduce the active acid content in cells, compared to any of a single component uridylic acid, a single component adenylic acid, a single component yeast peptide, a combination of yeast peptide and a functional ingredient only, a combination of two types of nucleotides (5'-uridylic acid and 5'-adenylic acid combined in a weight ratio of 1:1), a combination of two types of nucleotides and yeast peptide (5'-uridylic acid, 5'-adenylic acid, and yeast peptide combined in a weight ratio of 0.6:0.6:0.25), or a combination of 5'-guanylic acid, 5'-cytidylic acid, and yeast peptide. Uridylic acid and adenylic acid act synergistically with yeast peptides to enhance the ability to reduce intracellular active oxygen, and the effect is further improved after combining functional ingredients.

[0039] Example 9 Effect of a composition containing uridylic acid, adenylic acid and yeast peptides on D-galactose-induced mice Five-week-old BALB / c mice were bred for one week, and 20 of them were intraperitoneally injected with 50 mg / kg of D-galactose for 30 days. After 30 days, the mice were divided into groups of 10 mice each. Experimental group: Each of the compositions of Examples 1 to 6 was administered intragastrically for 28 days, and D-galactose was simultaneously injected intraperitoneally. Control group 1: 5'-uridylic acid was administered intragastrically for 28 days in the same volume as the composition, and D-galactose was injected intraperitoneally. Control group 2: 5'-adenylic acid was administered intragastrically for 28 days in the same volume as the composition, and D-galactose was injected intraperitoneally. Control group 3: The same volume of yeast peptide as the composition was administered intragastrically for 28 days, and D-galactose was injected intraperitoneally. Control group 4: A compounded composition containing 0.6 parts by weight of 5'-uridylic acid and 0.6 parts by weight of 5'-adenylic acid in the same volume as the composition was administered intragastrically for 28 days, and D-galactose was also administered intraperitoneally. Control group 5: A compounded composition containing 0.6 parts by weight of 5'-uridylic acid, 0.6 parts by weight of 5'-adenylic acid, and 0.25 parts by weight of yeast peptide in the same volume as the composition was administered intragastrically for 28 days, and D-galactose was also injected intraperitoneally. Control group 6: A compounded composition containing 0.6 parts by weight of 5'-guanylic acid, 0.6 parts by weight of 5'-cytidylic acid, and 0.25 parts by weight of yeast peptide in the same volume as the composition was administered intragastrically for 28 days, and D-galactose was also injected intraperitoneally. Control group 7: A compound composition containing 0.25 parts by weight of yeast peptide, 2 parts by weight of antler extract, 0.02 parts by weight of pyrroloquinoline quinone, 0.25 parts by weight of glutathione, 0.19 parts by weight of curcumin, 0.5 parts by weight of tea polyphenol, 0.5 parts by weight of quercetin, and 0.051 parts by weight of resveratrol in the same volume as the composition was administered intragastrically for 28 days, and D-galactose was injected intraperitoneally. Control group 8: A compounded composition containing 0.25 parts by weight of yeast peptide, 0.02 parts by weight of pyrroloquinoline quinone, 0.25 parts by weight of glutathione, 0.19 parts by weight of curcumin, 0.5 parts by weight of tea polyphenol, 0.5 parts by weight of quercetin, and 0.051 parts by weight of resveratrol in the same volume as the composition was administered intragastrically for 28 days, and D-galactose was injected intraperitoneally. Model group: PBS buffer solution with the same volume as the composition of Examples 1 to 6 was administered intragastrically. Blank group: Physiological saline in the same amount as D-galactose was intraperitoneally injected, and after 30 days, PBS buffer in the same volume as the compositions of Examples 1 to 6 was intragastrically administered for 28 days, and intraperitoneal injection was also continued for 28 days.

[0040] The day after intragastric administration is completed, orbital blood is collected for subsequent experiments.

[0041] According to the experimental results shown in Figures 1 to 3, the contents of oxidase SOD and GSH-PX in serum were increased, and malondialdehyde was reduced. This verified that the composition of the present invention can alleviate the oxidative damage of D-galactose in mice. The compositions described in embodiments 1 to 6 show stronger resistance to oxidative damage compared to the control groups 1 to 8. Uridylic acid and adenylic acid act synergistically with yeast peptides to further improve the compounding effect of functional ingredients.

[0042] Example 10 Slowing effect of a composition containing uridylic acid, adenylic acid and yeast peptides on natural aging in mice Eight-week-old BALB / c mice are randomly divided into two groups, with 10 mice in each group. Experimental group: Each of the compositions of Examples 1 to 6 was administered intragastrically to the mice until they died due to natural aging. Blank group: The same amount of physiological saline as that of the composition was administered intragastrically to the mice until they died due to natural aging. Control group 1: 5'-uridylic acid was administered intragastrically in the same volume as the composition until the mice died due to natural aging. Control group 2: 5'-adenylic acid was administered intragastrically in the same volume as the composition until the mice died of natural aging. Control group 3: The same volume of yeast peptide as the composition was administered intragastrically to the mice until they died due to natural aging. Control group 4: A compounded composition containing 0.6 parts by weight of 5'-uridylic acid and 0.6 parts by weight of 5'-adenylic acid in the same volume as the composition was administered intragastrically until the mice died due to natural aging. Control group 5: A compound composition containing 0.6 parts by weight of 5'-uridylic acid, 0.6 parts by weight of 5'-adenylic acid, and 0.25 parts by weight of yeast peptide in the same volume as the composition was administered intragastrically until the mice died due to natural aging. Control group 6: A compounded composition containing 0.6 parts by weight of 5'-guanylic acid, 0.6 parts by weight of 5'-cytidylic acid, and 0.25 parts by weight of yeast peptide in the same volume as the composition was administered intragastrically until the mice died due to natural aging. Control group 7: A compound composition containing 0.25 parts by weight of yeast peptide, 2 parts by weight of antler extract, 0.02 parts by weight of pyrroloquinoline quinone, 0.25 parts by weight of glutathione, 0.19 parts by weight of curcumin, 0.5 parts by weight of tea polyphenol, 0.5 parts by weight of quercetin, and 0.051 parts by weight of resveratrol in the same volume as the composition was administered intragastrically until the mice died of natural aging. Control group 8: A compound composition containing 0.25 parts by weight of yeast peptide, 0.02 parts by weight of pyrroloquinoline quinone, 0.25 parts by weight of glutathione, 0.19 parts by weight of curcumin, 0.5 parts by weight of tea polyphenol, 0.5 parts by weight of quercetin, and 0.051 parts by weight of resveratrol in the same volume as the composition was administered intragastrically until the mice died of natural aging.

[0043] The lifespan of the mice in each group was statistically shown in Figure 4. The results shown in Figure 4 show that the composition containing uridylic acid, adenylic acid and yeast peptides described in the present invention can effectively delay the natural aging of mice.

[0044] Anyone skilled in the art can make many possible changes and modifications to the technical means of the present invention based on the technical content shown above without departing from the scope of the technical means of the present invention, or modify it into an equivalent embodiment that corresponds to an equivalent modification. Therefore, simple changes, equivalent modifications and modifications made to the above embodiments according to the technical contents of the present invention without departing from the scope of the technical means of the present invention should still be included in the protection scope of the present invention.

[0045] (Additional Note) (Appendix 1) functional active ingredients including one or more of 5'-uridylic acid, 5'-adenylic acid, yeast peptides, antler extract, pyrroloquinoline quinone, glutathione, curcumin, tea polyphenols, quercetin, resveratrol, alpha-lipoic acid, alpha-ketoglutaric acid, and fisetin; Including, An anti-aging composition comprising uridylic acid, adenylic acid and a yeast peptide.

[0046] (Appendix 2) In parts by weight, it contains 0.3-1.2 parts of 5'-uridylic acid, 0.3-1.2 parts of 5'-adenylic acid, 0.05-1.5 parts of yeast peptides, and 0.88-7.35 parts of functional active ingredients; The composition according to claim 1,

[0047] (Appendix 3) The functional active ingredient is The composition contains, by weight, one or more of the following: 1-2 parts deer antler extract, 0.01-0.05 parts pyrroloquinoline quinone, 0.25-1 parts glutathione, 0.12-0.6 parts curcumin, 0.5-1 parts tea polyphenols, 0.5-1.25 parts quercetin, 0.02-0.1 parts resveratrol, 0.01-0.1 parts alpha-lipoic acid, 0.3-1 parts alpha-ketoglutaric acid, and 0.05-0.25 parts fisetin. The composition according to claim 1,

[0048] (Appendix 4) A composition comprising, by weight, 0.6 parts of 5'-uridylic acid, 0.6 parts of 5'-adenylic acid, 0.25 parts of yeast peptide, 2 parts of antler extract, 0.02 parts of pyrroloquinoline quinone, 0.25 parts of glutathione, 0.19 parts of curcumin, 0.5 parts of tea polyphenols, 0.5 parts of quercetin, and 0.051 parts of resveratrol; or The composition contains, by weight, 0.6 parts 5'-uridylic acid, 0.6 parts 5'-adenylic acid, 0.25 parts yeast peptide, 2 parts antler extract, 0.05 parts alpha-lipoic acid, 0.3 parts alpha-ketoglutaric acid, 0.02 parts pyrroloquinoline quinone, 0.19 parts curcumin, 0.1 parts fisetin, 0.25 parts glutathione, and 0.051 parts resveratrol. 4. The composition according to any one of claims 1 to 3,

[0049] (Appendix 5) or comprising, by weight, 0.3 parts 5'-uridylic acid, 0.3 parts 5'-adenylic acid, 0.17 parts yeast peptide, 0.01 parts pyrroloquinoline quinone, 0.25 parts glutathione, 0.12 parts curcumin, and 0.5 parts tea polyphenols; or or comprising, by weight, 0.6 parts 5'-uridylic acid, 0.6 parts 5'-adenylic acid, 0.25 parts yeast peptide, 0.02 parts pyrroloquinoline quinone, 0.25 parts glutathione, 0.19 parts curcumin, 0.5 parts tea polyphenols, 0.5 parts quercetin, and 0.051 parts resveratrol; or or comprising, by weight, 0.3 parts 5'-uridylic acid, 0.3 parts 5'-adenylic acid, 0.17 parts yeast peptide, 0.05 parts fisetin, 0.01 parts pyrroloquinoline quinone, 0.25 parts glutathione, 0.12 parts curcumin, and 0.5 parts tea polyphenols; or The composition contains, by weight, 0.6 parts 5'-uridylic acid, 0.6 parts 5'-adenylic acid, 0.25 parts yeast peptides, 0.05 parts alpha-lipoic acid, 0.3 parts alpha-ketoglutaric acid, 0.02 parts pyrroloquinoline quinone, 0.19 parts curcumin, 0.1 parts fisetin, 0.25 parts glutathione, and 0.051 parts resveratrol. 4. The composition according to any one of claims 1 to 3,

[0050] (Appendix 6) 5. The composition according to claim 1, 3 or 4, wherein the antler extract is derived from fresh antler.

[0051] (Appendix 7) 7. The composition according to any one of claims 1 to 6, characterized in that the finished product is in the form of a powder, granules, tablet, pill, oral liquid or gelatin capsule.

[0052] (Appendix 8) Use of an anti-aging composition comprising uridylic acid, adenylic acid and yeast peptide according to any one of appendices 1 to 7 in the manufacture of an anti-aging drug, health care product or nutritional food.

[0053] (Appendix 9) The use according to claim 8, wherein the medicine, health care product or nutritional food has the effects of removing free radicals in the human body, anti-oxidation, good protection and repair for damaged cells, delaying aging and restoring vitality to the human body.

Claims

1. functional active ingredients including one or more of 5'-uridylic acid, 5'-adenylic acid, yeast peptides, antler extract, pyrroloquinoline quinone, glutathione, curcumin, tea polyphenols, quercetin, resveratrol, α-lipoic acid, α-ketoglutaric acid, and fisetin; Including, An anti-aging composition comprising uridylic acid, adenylic acid and a yeast peptide.

2. In parts by weight, it contains 0.3-1.2 parts of 5'-uridylic acid, 0.3-1.2 parts of 5'-adenylic acid, 0.05-1.5 parts of yeast peptides, and 0.88-7.35 parts of functional active ingredients. The composition of claim 1 .

3. The functional active ingredient is The composition contains, in parts by weight, one or more of the following: 1-2 parts deer antler extract, 0.01-0.05 parts pyrroloquinoline quinone, 0.25-1 parts glutathione, 0.12-0.6 parts curcumin, 0.5-1 parts tea polyphenols, 0.5-1.25 parts quercetin, 0.02-0.1 parts resveratrol, 0.01-0.1 parts alpha-lipoic acid, 0.3-1 parts alpha-ketoglutaric acid, and 0.05-0.25 parts fisetin. The composition of claim 1 .

4. By weight, it contains 0.6 parts of 5'-uridylic acid, 0.6 parts of 5'-adenylic acid, 0.25 parts of yeast peptide, 2 parts of antler extract, 0.02 parts of pyrroloquinoline quinone, 0.25 parts of glutathione, 0.19 parts of curcumin, 0.5 parts of tea polyphenols, 0.5 parts of quercetin, and 0.051 parts of resveratrol; or The composition contains, by weight, 0.6 parts 5'-uridylic acid, 0.6 parts 5'-adenylic acid, 0.25 parts yeast peptide, 2 parts antler extract, 0.05 parts alpha-lipoic acid, 0.3 parts alpha-ketoglutaric acid, 0.02 parts pyrroloquinoline quinone, 0.19 parts curcumin, 0.1 parts fisetin, 0.25 parts glutathione, and 0.051 parts resveratrol. The composition according to any one of claims 1 to 3.

5. or comprising, by weight, 0.3 parts 5'-uridylic acid, 0.3 parts 5'-adenylic acid, 0.17 parts yeast peptide, 0.01 parts pyrroloquinoline quinone, 0.25 parts glutathione, 0.12 parts curcumin, and 0.5 parts tea polyphenols; or or comprising, by weight, 0.6 parts 5'-uridylic acid, 0.6 parts 5'-adenylic acid, 0.25 parts yeast peptide, 0.02 parts pyrroloquinoline quinone, 0.25 parts glutathione, 0.19 parts curcumin, 0.5 parts tea polyphenols, 0.5 parts quercetin, and 0.051 parts resveratrol; or or comprising, by weight, 0.3 parts 5'-uridylic acid, 0.3 parts 5'-adenylic acid, 0.17 parts yeast peptide, 0.05 parts fisetin, 0.01 parts pyrroloquinoline quinone, 0.25 parts glutathione, 0.12 parts curcumin, and 0.5 parts tea polyphenols; or The composition contains, by weight, 0.6 parts 5'-uridylic acid, 0.6 parts 5'-adenylic acid, 0.25 parts yeast peptides, 0.05 parts alpha-lipoic acid, 0.3 parts alpha-ketoglutaric acid, 0.02 parts pyrroloquinoline quinone, 0.19 parts curcumin, 0.1 parts fisetin, 0.25 parts glutathione, and 0.051 parts resveratrol. The composition according to any one of claims 1 to 3.

6. 5. The composition according to claim 1, 3 or 4, wherein the antler extract is derived from fresh antler.

7. The composition according to any one of claims 1 to 6, characterized in that the finished product is in the form of a powder, granules, tablet, pill, oral liquid or gelatin capsule.

8. Use of an anti-aging composition comprising uridylic acid, adenylic acid and yeast peptide according to any one of claims 1 to 7 in the manufacture of anti-aging medicines, health care products or nutritional foods.

9. The use according to claim 8, characterized in that the medicine, the health care product or the nutritional food has the effects of removing free radicals in the human body, anti-oxidation, good protection and repair for damaged cells, delaying aging and restoring vitality to the human body.

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