Topical formulations useful for improving hair health or scalp health or for treating or preventing hair loss

Abstract

The present disclosure relates to topical formulations (scalp essences, scalp cleansers, scalp masks, etc.) for balancing the scalp microbiome and their use for improving hair health or treating or preventing hair loss.

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Patent Application No. 63 / 337,011, filed April 29, 2022, which is incorporated by reference in its entirety herein.

[0002] The present disclosure relates to topical formulations (scalp essences, scalp washes, scalp masks, etc.) for balancing the scalp microbiome and their use for treating hair conditions. [Background technology]

[0003] Human body surfaces (skin and scalp) provide an ecological niche that is home to a large number of diverse bacteria and fungi, constituting the "microbiome". Microbiome dysbiosis, which results in a bias in bacterial abundance, is associated with scalp and skin disorders, which highlights the role of host-bacterial community interdependence. It is increasingly evident that the scalp creates a unique microenvironment for bacterial colonization, forming its own unique microbiome that is independent of the skin microbiome. Summary of the Invention [Problem to be solved by the invention]

[0004] There are limited examples of therapies and ingredients that target the microbiome to rebalance unhealthy conditions and thus positively impact scalp / skin health. Due to the complex and dynamic nature of the microbiome, there are currently no known strategies to create microbiome-targeted therapies. [Means for solving the problem]

[0005] In one aspect, a composition is provided that includes about 0.1% to 0.3% by weight of sarcosine, about 0.01% to 0.1% by weight of kunzea pomifera or an extract thereof, about 0.01% to 0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% by weight of tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% by weight of pea (pisum sativum) or an extract thereof, and about 0.01% to 0.1% by weight of chia (salvia hispanica) or an extract thereof.

[0006] Also provided herein is a method of improving hair health or scalp health, or treating or preventing hair loss, in a subject in need thereof, comprising applying an effective amount of the composition to the scalp of the subject.

[0007] In another aspect, provided herein is a composition comprising about 0.01% to 0.1% by weight of α-glucan oligosaccharides, about 0.01% to 0.1% by weight of kunzea pomifera or an extract thereof, about 0.01% to 0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, and about 0.01% to 0.1% by weight of Tasmannia lanceolata or an extract thereof.

[0008] Further provided herein is a method of improving hair health or scalp health, or treating or preventing hair loss in a subject in need thereof, comprising applying an effective amount of the composition to the scalp of the subject.

[0009] In another aspect, provided herein is a composition comprising about 1% to 3% by weight of α-glucan oligosaccharides, about 0.1% to 0.3% by weight of Bacillus fermentation filtrate or an extract thereof, about 0.1% to 0.3% by weight of Opuntia ficus-indica or an extract thereof, and about 0.01% to 0.1% by weight of peach gum (Prunus persica gum).

[0010] Further provided herein is a method for improving hair health or scalp health or treating or preventing hair loss in a subject in need thereof, comprising applying to the scalp of the subject an effective amount of the composition. [Brief description of the drawings]

[0011] [Figure 1A] ~ [Figure 1C] FIG. 1 shows the effect of scalp essence, scalp cleanser, and scalp exfoliating mask, respectively, on the growth (Cfu / mL as % of control) of co-cultures of S. epidermidis (red) and S. aureus (blue) normalized to the control (100% growth). [Figure 2A] ~ [Figure 2C] Figure 14 shows the effect of scalp essence, scalp cleanser, and scalp exfoliating mask, respectively, on the microbial diversity (CFU / mL as % of control) of major scalp microorganisms in co-culture. [Figure 3A] ~ [Figure 3C] Figure 1 shows the growth behaviour of individual microorganisms (as % of control) in the presence of scalp essence, scalp cleanser and scalp exfoliating mask, respectively. [Figure 4] FIG. 1 shows a scalp photograph clinical rating scale that can be used to assess the efficacy of the methods described herein. [Figure 5A] and [Figure 5B]Figure 5 depicts sebum content measured on subject scalp (N=37) over a 14-day sample shampoo (washout) usage period for either the entire cohort (Figure 5A) or stratified by dry and oily groups (Figure 5B). [Figure 6A] and [Figure 6B] FIG. 6 shows scalp pH in subjects (N=37) measured over time over 14 days of sample shampoo use, either for the entire cohort (FIG. 6A) or stratified by dry and oily groups (FIG. 6B). [Figure 7A] and [Figure 7B] FIG. 7B shows scalp hydration in subjects (N=37) as measured by corneometer over 14 days of sample shampoo use, either for the entire cohort (FIG. 7A) or stratified by dry and oily groups (FIG. 7B). [Figure 8A] and [Figure 8B] FIG. 1 shows scalp dandruff in subjects, as measured by expert scores from dermatologists, over a 14-day period using sample shampoos. [Figure 9A] and [Figure 9B] 9A-9B show changes in overall subject scalp sebum measurements from baseline after use of the sample mask as measured by a sebumeter (FIG. 9A) or expert grading (FIG. 9B). [Figure 10] FIG. 1 shows scalp pH measured over time using a sample mask for 14 days. [Figure 11] FIG. 1 shows scalp moisture retention measured with a corneometer after using a sample essence for 14 days. [Figure 12] FIG. 1 shows the sebum content measured with a sebum meter after using a sample essence for 28 days. [Figure 13] FIG. 1 shows scalp pH measured over a 14 day period using a sample essence. [Figure 14A] and [Figure 14B]Figure 14 shows hair loss measured by the total number of hairs in the four quadrants collected from the brush over a 28 day period using the essence, Figure 14A shows the total number of hairs and Figure 14B shows the hair loss measured by the number of hairs with the parameters root, no root and total. [Figure 15] FIG. 1 shows the results of expert clinical grading, dandruff, and redness after 28 days of using the essence. [Figure 16] Figure 1 shows the change in sebum content over time in the dry and oily groups, where dryness and oiliness were judged by an expert assessor. The sebum level in the dry group decreased by 32% over the 14 days of using the sample shampoo. The oily group (baseline sebum > 50) decreased by 37% over the 14 days of using the sample shampoo. [Figure 17] This is a diagram showing the change in pH in the dry and oily groups after 14 days of use of the sample shampoo. The dry and oily groups were determined by expert evaluation. There was no change in scalp pH due to the use of the sample shampoo (overall % change <2%). [Figure 18] Figure 1 shows scalp moisture retention as measured by a corneometer over the 14-day period when the sample shampoo was used for the dry and oily scalp groups, which were assessed by a specialist evaluator. Moisture retention in the dry scalp group improved by 25% over the 14-day period when the sample shampoo was used. Moisture retention in the oily scalp group improved by 19% over the 14-day period when the sample shampoo was used. [Figure 19] This is a diagram showing the scalp dandruff measured by a specialist evaluator (dermatologist) after using the sample shampoo for dry and oily groups for 14 days. The dry and oily groups were judged by a specialist evaluator. After using the sample shampoo for 14 days, the dandruff grading by the expert showed a 74% improvement in the dry group and an 83% improvement in the oily group. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] Without wishing to be bound by theory, it is believed that the compositions and methods herein promote a diverse scalp microbiota, which may lead to a healthier scalp due to the symbiotic host-microbe relationship, and thus promote a balanced and diverse microbial population on the scalp, which is useful for improving hair and scalp health or treating or preventing hair loss.

[0013] definition As used herein, the term "about" when immediately preceding a numerical value means ± up to 20% of that numerical value. For example, "about" a numerical value means ± up to 20% of that numerical value, and in some embodiments, ± up to 19%, ± up to 18%, ± up to 17%, ± up to 16%, ± up to 15%, ± up to 14%, ± up to 13%, ± up to 12%, ± up to 11%, ± up to 10%, ± up to 9%, ± up to 8%, ± up to 7%, ± up to 6%, ± up to 5%, ± up to 4%, ± up to 3%, ± up to 2%, ± up to 1%, ± less than up to 1%, or other values ​​or ranges of values ​​thereof. In some embodiments, "about" a numerical value means ± up to 10% of that numerical embodiment. As used herein, a "subject" is a mammal, such as, for example, a dog, cat, gerbil, horse, sheep, goat, or human.

[0014] composition Provided herein are compositions useful for improving hair health or scalp health or treating or preventing hair loss. Examples of improving hair health or scalp health include hydrating hair or scalp, rebalancing hair or scalp microenvironment, microbiota and / or pH, reducing sebum accumulation on hair or scalp, reducing scalp inflammation or irritation, and reducing dandruff. In some embodiments, the composition is formulated for use as a scalp mask. In some embodiments, the composition is formulated for use as a scalp cleanser, such as a shampoo. In some embodiments, the composition is formulated for use as a scalp essence. Unless otherwise specified, the amount of ingredients present in the composition is described using weight percent ("wt %), which is the weight of the ingredient compared to the weight of the entire composition.

[0015] Provided herein are compositions that can be used to exfoliate and / or rebalance the scalp. In some embodiments, the compositions are useful as scalp masks. Exemplary compositions useful as scalp masks are shown in Table 1. Without wishing to be bound by theory, it is believed that the compositions help balance the scalp environment and thus contribute to healthier hair growth conditions. Thus, in some embodiments, the compositions provided herein can reduce excess sebum and oil that accumulates on the scalp. In some embodiments, the compositions moisturize the scalp. In some embodiments, the scalp masks lead to rebalancing the scalp microenvironment and microflora.

[0016] In some embodiments, the compositions provided herein include sarcosine. Without being bound by theory, sarcosine, an amino acid derivative, is believed to provide the ability to reduce sebum and regulate microflora. By reducing the sebum content of oily scalp, sarcosine is believed to effectively reduce the nutrients available to lipophilic bacteria and yeasts, such as P. acnes and / or Malassezia species. Sarcosine can also increase the microbial diversity of the scalp over time, which is believed to help build a healthier scalp environment for hair health, scalp health, or hair loss treatment or prevention. Sarcosine may be present in the compositions provided herein at a concentration of about 0.05% to 0.5%, such as about 0.05% to 0.1%, about 0.05% to 0.2%, about 0.05% to 0.3%, about 0.05% to 0.4%, about 0.1% to 0.2%, about 0.1% to 0.3%, about 0.1% to 0.4%, or about 0.1% to 0.5%. In some embodiments, sarcosine is present in the scalp mask at a concentration of about 0.1% to 0.3%.

[0017] In some embodiments, the compositions provided herein include one or more plants, one or more plant parts, such as roots, leaves, stems, seeds, flowers, or fruits, such as berries, or extracts thereof, such as wildberry extracts. Wildberries or extracts thereof (e.g., blends or mixtures of kunzea pomifera or extracts thereof, ryeberry (syzygium luehmannii) or extracts thereof, and tasmannia lanceolata or extracts thereof) are believed to promote scalp hydration and relieve dry scalp conditions. The kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, and / or tasmannia lanceolata extract can each be a fruit extract, leaf extract, root extract, stem extract, seed extract, flower extract, or combinations thereof. In some embodiments, the kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, and / or tasmannia lanceolata extract are whole plant extracts. The kunzea pomifera or extract thereof, ryeberry (syzygium luehmannii) or extract thereof, and tasmannia lanceolata extract can each be a fruit extract, leaf extract, root extract, stem extract, seed extract, flower extract, or combinations thereof. In some embodiments, the kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, and / or tasmannia lanceolata extract are whole plant extracts. lanceolata) or an extract thereof can be present in the composition at a concentration of about 0.005% to 0.5%, e.g., about 0.005% to 0.01%, about 0.005% to 0.02%, about 0.005% to 0.03%, about 0.005% to 0.04%, about 0.005% to 0.05%, about 0.01% to 0.02%, about 0.01% to 0.03%, about 0.01% to 0.04%, about 0.01% to 0.05%, about 0.01% to 0.1%, about 0.1% to 0.2%, about 0.1% to 0.3%, about 0.1% to 0.4%, or about 0.1% to 0.5%, respectively.In some embodiments, each of the kunzea pomifera or extract thereof, the ryeberry (syzygium luehmannii) or extract thereof, and the tasmannia lanceolata or extract thereof is present in the composition at a concentration of about 0.01% to 0.1%. In some embodiments, the kunzea pomifera or extract thereof, the ryeberry (syzygium luehmannii) or extract thereof, and the tasmannia lanceolata or extract thereof are present in about equal proportions. In some embodiments, the compositions provided herein include a blend or mixture of pea (pisum sativum) (pea) or an extract thereof and chia (salvia hispanica) or an extract thereof. The blend or mixture of pea (pisum sativum) or an extract thereof and chia (salvia hispanica) or an extract thereof is believed to increase scalp hydration by reducing the sebum content and transepidermal water loss of the scalp. The pea (pisum sativum) extract and / or chia (salvia hispanica) extract can each be a fruit extract, leaf extract, root extract, stem extract, seed extract, flower extract, or combination thereof. In some embodiments, the pea (pisum sativum) extract and / or chia (salvia hispanica) extract are whole plant extracts. hispanica) or an extract thereof can be present in the composition at a concentration of about 0.005% to 0.5%, e.g., about 0.005% to 0.01%, about 0.005% to 0.02%, about 0.005% to 0.03%, about 0.005% to 0.04%, about 0.005% to 0.05%, about 0.01% to 0.02%, about 0.01% to 0.03%, about 0.01% to 0.04%, about 0.01% to 0.05%, about 0.01% to 0.1%, about 0.1% to 0.2%, about 0.1% to 0.3%, about 0.1% to 0.4%, or about 0.1% to 0.5%, respectively. In some embodiments, each of pea (pisum sativum) or an extract thereof and chia (salvia hispanica) or an extract thereof is present in the composition at a concentration of about 0.01% to 0.1%.In some embodiments, the pea (pisum sativum) or extract thereof and chia (salvia hispanica) or extract thereof are present in approximately equal proportions.

[0018] Table 1: Exemplary compositions useful as scalp masks [Table 1]

[0019] In some embodiments, the compositions provided herein include about 0.1% to 0.3% sarcosine, about 0.01% to 0.1% kunzea pomifera or an extract thereof, about 0.01% to 0.1% ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% pea (pisum sativum) or an extract thereof, and about 0.01% to 0.1% chia (salvia hispanica) or an extract thereof.

[0020] In some embodiments, the compositions provided herein consist essentially of about 0.1%-0.3% sarcosine, about 0.01%-0.1% kunzea pomifera or an extract thereof, about 0.01%-0.1% ryeberry (syzygium luehmannii) or an extract thereof, about 0.01%-0.1% tasmannia lanceolata or an extract thereof, about 0.01%-0.1% pea (pisum sativum) or an extract thereof, and about 0.01%-0.1% chia (salvia hispanica) or an extract thereof.

[0021] In some embodiments, the composition further comprises about 0.1% to 0.3% of bilberry (vaccinium myrtillus) or an extract thereof, about 0.1% to 0.3% of saccharum officinarum (sugar cane) or an extract thereof, about 0.01% to 0.1% of citrus aurantium dulcis (orange) or an extract thereof, about 0.01% to 0.1% of citrus limon (lemon) or an extract thereof, about 0.01% to 0.1% of acer saccharum (sugar maple) or an extract thereof, and about 1% to 3% of jojoba esters, Simmondsia chinensis (Simmondsia chinensis) or an extract thereof. chinensis (Vaccinium myrtillus) extract, Saccharum officinarum (Sugar cane) extract, Citrus aurantium dulcis (Orange) extract, Citrus limon (Lemon) extract, and / or Acer saccharum (Sugar maple) extract each can be a fruit extract, leaf extract, root extract, stem extract, seed extract, flower extract, or combinations thereof. In some embodiments, vaccinium myrtillus extract, saccharum officinarum (sugar cane) extract, citrus aurantium dulcis (orange) extract, citrus limon (lemon) extract, and / or acer saccharum (sugar maple) extract are whole plant extracts.

[0022] In some embodiments, the compositions provided herein consist essentially of about 0.1% to 0.3% sarcosine, about 0.01% to 0.1% kunzea pomifera or an extract thereof, about 0.01% to 0.1% ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% pea (pisum sativum) or an extract thereof, about 0.01% to 0.1% chia (salvia hispanica) or an extract thereof, about 0.3% vaccinium myrtillus (vaccinium myrtillus), about 0.01% to 0.1% sarcosine, about 0.01% to 0.1% kunzea pomifera or an extract thereof, about 0.01% to 0.1% ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% pea (pisum sativum) or an extract thereof, about 0.01% to 0.1% chia (salvia hispanica) or an extract thereof, about 0.3% vaccinium myrtillus (vaccinium myrtillus))) or extract thereof, about 0.1% to 0.3% saccharum officinarum (sugar cane) or extract thereof, about 0.01% to 0.1% citrus aurantium dulcis (orange) or extract thereof, about 0.01% to 0.1% citrus limon (lemon) or extract thereof, about 0.01% to 0.1% acer saccharum (sugar maple) or extract thereof, and about 1% to 3% jojoba esters, Simmondsia chinensis or extract thereof.

[0023] In some embodiments, the compositions provided herein further comprise glycerin, cetearyl alcohol, brassicamidopropyl dimethylamine, fragrance, polyquatemium-37, lactic acid, sodium benzoate, piroctone olamine, potassium sorbate, tetrasodium glutamate diacetate, montmorillonite, CI 77289 (chromium hydroxide green), isopropyl alcohol, sodium hydroxide, tetrasodium EDTA, and / or Aspergillus ferment.

[0024] Glycerin may be present in the composition at a concentration of about 1% to 20%, and in some embodiments, at a concentration of about 3% to 10%.

[0025] Cetearyl alcohol, brassicamidopropyl dimethylamine, jojoba esters, Simmondsia chinensis or extract thereof, and / or fragrance may each be present in the composition at a concentration of about 0.5% to 5%, and in some embodiments, about 1% to 3%.

[0026] Polyquatemium-37, lactic acid, and / or sodium benzoate may each be present in the composition at a concentration of about 0.1% to 1%, and in some embodiments, at a concentration of about 0.3% to 0.99%.

[0027] Vaccinium myrtillus or an extract thereof, piroctone olamine, potassium sorbate, and / or saccharum officinarum (sugar cane) or an extract thereof may each be present in the composition at a concentration of about 0.05% to 0.5%, and in some embodiments, about 0.1% to 0.3%.

[0028] Tetrasodium glutamic acid diacetate, montmorillonite, citrus aurantium dulcis (orange) or extract thereof, citrus limon (lemon) or extract thereof, CI77289 (chromium hydroxide green), acer saccharum (sugar maple) or extract thereof, isopropyl alcohol, sodium hydroxide, tetrasodium EDTA, and / or Aspergillus ferment may each be present in the composition at a concentration of about 0.005% to 0.5%, and in some embodiments, about 0.01% to 0.1%.

[0029] In some embodiments, the composition (e.g., useful as a scalp mask) further comprises water. The water can form the base of the composition. In some embodiments, the water is present in a concentration of at least about 30%, up to about 90%, up to about 95%, up to about 96%, up to about 97%, up to about 98%, or up to about 99%.

[0030] Provided herein is a composition that can be used to cleanse the scalp without destroying symbiotic bacteria. In some embodiments, such compositions are formulated for use as scalp cleansers. Exemplary compositions useful as scalp cleansers are provided in Table 2. Without wishing to be bound by theory, it is believed that such compositions can gently cleanse the scalp without stripping symbiotic bacteria, creating a clean slate for gently resetting the scalp condition. Thus, in some embodiments, the compositions provided herein reset the scalp microenvironment. In some embodiments, the compositions formulated for use as scalp cleansers are useful as shampoos.

[0031] In some embodiments, the compositions provided herein include α-glucan oligosaccharides, which are microbiota-modulating prebiotics. Without being bound by theory, it is believed that α-glucan oligosaccharides suppress the growth of commensal bacteria without exfoliation or strong antibacterial effects, thus increasing the presence of beneficial bacteria such as Staphylococcus epidermidis, and also increasing microbial biodiversity. The α-glucan oligosaccharides may be present in the composition at a concentration of about 0.005% to 0.5%, for example, about 0.005% to 0.01%, about 0.005% to 0.02%, about 0.005% to 0.03%, about 0.005% to 0.04%, about 0.005% to 0.05%, about 0.01% to 0.02%, about 0.01% to 0.03%, about 0.01% to 0.04%, about 0.01% to 0.05%, about 0.01% to 0.1%, about 0.1% to 0.2%, about 0.1% to 0.3%, about 0.1% to 0.4%, or about 0.1% to 0.5%. In some embodiments, the α-glucan oligosaccharides are present in the composition at a concentration of about 0.01% to 0.1%.

[0032] In some embodiments, the compositions provided herein (e.g., useful as scalp cleansing agents) include one or more plants; one or more plant parts, such as roots, leaves, stems, seeds, flowers, or fruits, such as berries; or extracts thereof, such as wildberry extracts. A blend or mixture of wildberries or extracts thereof (in some embodiments, a blend or mixture of kunzea pomifera or extracts thereof, ryeberry (syzygium luehmannii) or extracts thereof, and tasmannia lanceolata or extracts thereof) is believed to increase scalp moisture retention and help relieve dry scalp conditions. The kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, and / or tasmannia lanceolata extract can each be a fruit extract, leaf extract, root extract, stem extract, seed extract, flower extract, or combinations thereof. In some embodiments, the kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, and / or tasmannia lanceolata extract are whole plant extracts.

[0033] Kunzea pomifera or an extract thereof, ryeberry (Syzygium luehmannii) or an extract thereof, and Tasmannia lanceolata lanceolata) or an extract thereof can be present in the composition at a concentration of about 0.005% to 0.5%, e.g., about 0.005% to 0.01%, about 0.005% to 0.02%, about 0.005% to 0.03%, about 0.005% to 0.04%, about 0.005% to 0.05%, about 0.01% to 0.02%, about 0.01% to 0.03%, about 0.01% to 0.04%, about 0.01% to 0.05%, about 0.01% to 0.1%, about 0.1% to 0.2%, about 0.1% to 0.3%, about 0.1% to 0.4%, or about 0.1% to 0.5%, respectively. In some embodiments, each of the kunzea pomifera or extract thereof, ryeberry (syzygium luehmannii) or extract thereof, and tasmannia lanceolata or extract thereof is present in the composition at a concentration of about 0.01% to 0.1%. In some embodiments, the kunzea pomifera or extract thereof, ryeberry (syzygium luehmannii) or extract thereof, and tasmannia lanceolata or extract thereof are present in about equal proportions.

[0034] Table 2: Exemplary compositions useful as scalp cleansers [Table 2]

[0035] In some embodiments, the compositions provided herein include about 0.01% to 0.1% α-glucan oligosaccharides, about 0.01% to 0.1% kunzea pomifera or an extract thereof, about 0.01% to 0.1% ryeberry (syzygium luehmannii) or an extract thereof, and about 0.01% to 0.1% tasmannia lanceolata or an extract thereof.

[0036] In some embodiments, the compositions provided herein consist essentially of about 0.01% to 0.1% α-glucan oligosaccharides, about 0.01% to 0.1% kunzea pomifera or an extract thereof, about 0.01% to 0.1% ryeberry (syzygium luehmannii) or an extract thereof, and about 0.01% to 0.1% tasmannia lanceolata or an extract thereof.

[0037] In some embodiments, the composition further comprises about 0.01% to 0.1% hydrolyzed quinoa and about 0.01% to 0.1% Aspergillus fermentate.

[0038] In some embodiments, the compositions provided herein consist essentially of about 0.01% to 0.1% α-glucan oligosaccharides, about 0.01% to 0.1% kunzea pomifera or an extract thereof, about 0.01% to 0.1% ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% hydrolyzed quinoa, and about 0.01% to 0.1% Aspergillus fermentate.

[0039] In some embodiments, the composition further comprises sodium C14-16 olefin sulfonate, cocamidopropyl betaine sodium lauroyl sarcosinate, propanediol, sodium chloride, fragrance, cocamide mipa, glycol distearate, citric acid, hydroxypropyl methylcellulose, guar hydroxypropyltrimonium chloride, glycerin, sodium benzoate, polyquaternium-73, caprylyl / capryl glucoside, potassium sorbate, glycolipids, tetrasodium glutamate diacetate, diisopropyl adipate, sodium hydroxide, triethyl citrate, caprylyl glyceryl ether, and / or benzyl alcohol.

[0040] Sodium C14-16 olefin sulfonate, cocamidopropyl betaine, and / or sodium lauroyl sarcosinate are each present in the composition at a concentration of about 1% to 20%, and in some embodiments, at a concentration of about 3% to 10%.

[0041] Propanediol and / or sodium chloride may each be present in the composition at a concentration of about 0.5% to 5%, and in some embodiments, about 1% to 3%.

[0042] Fragrance, cocamide MIPA, glycol distearate, citric acid, hydroxypropyl methylcellulose, guar hydroxypropyltrimonium chloride, glycerin, sodium benzoate, and / or polyquaternium-73 may each be present in the composition at a concentration of about 0.1% to 1%, and in some embodiments, 0.3% to 0.99%.

[0043] Caprylyl / capryl glucoside, potassium sorbate and / or glycolipids may each be present in the composition at a concentration of about 0.05% to 0.5%, and in some embodiments, at a concentration of about 0.1% to 0.3%.

[0044] Tetrasodium glutamic acid diacetate, hydrolyzed quinoa, diisopropyl adipate, sodium hydroxide, triethyl citrate, caprylyl glyceryl ether, benzyl alcohol and / or Aspergillus ferment may be present in the composition at a concentration of up to about 0.5%, and in some embodiments, up to about 0.1%.

[0045] In some embodiments, the composition further comprises water. The water may form the base of the composition. In some embodiments, the water is present in a concentration of at least about 30%, up to about 90%, up to about 95%, up to about 96%, up to about 97%, up to about 98%, or up to about 99%.

[0046] Provided herein are compositions that can be used to rebalance the scalp microflora. In some embodiments, the compositions are formulated for use as scalp essences. Exemplary compositions useful as scalp essences are shown in Table 3. Without wishing to be bound by theory, it is believed that the compositions provide the ability to restore scalp and hair health. In particular, the soothing, sebum reducing, hydrating, and microbiota regulating ingredients are believed to contribute to a healthy, moisturized, restored scalp, which in turn promotes hair health. In some embodiments, the compositions provided herein reconstruct and restore scalp microenvironmental conditions by regulating microbes, increasing hydration, reducing oiliness, reducing inflammation, and / or soothing irritation. In some embodiments, the compositions are aqueous. In some embodiments, the term "essence" is substantially similar to "serum," and in such embodiments, these terms are used interchangeably herein.

[0047] In some embodiments, the compositions provided herein include α-glucan oligosaccharides, which are microbiota-modulating prebiotics. Without being bound by theory, it is believed that α-glucan oligosaccharides suppress the growth of commensal bacteria without exfoliative or strong antibacterial activity, thereby increasing the presence of beneficial bacteria such as Staphylococcus epidermidis, and also increasing microbial biodiversity. The α-glucan oligosaccharides can be present in the composition at a concentration of about 0.5% to 5%, for example, about 0.5% to 1%, about 0.5% to 2%, about 0.5% to 3%, about 0.5% to 4%, about 1% to 2%, about 1% to 3%, about 1% to 4%, or about 1% to 5%. In some embodiments, the α-glucan oligosaccharides are present in the composition at a concentration of about 1% to 3%.

[0048] In some embodiments, the compositions provided herein comprise one or more plants, one or more plant parts, such as roots, leaves, stems, seeds, flowers, or fruits, such as berries, or extracts thereof, such as wildberry extracts.

[0049] In some embodiments, the compositions provided herein include peach gum polysaccharides. Peach gum polysaccharides are believed to complement the regulation of the microflora by suppressing lipophilic microorganisms such as P. Acnes and M. Furfur. Peach gum polysaccharides are also believed to provide a secondary effect of soothing the scalp by reducing redness and visible inflammation. The peach gum polysaccharides may be present in the composition at a concentration of about 0.005% to 0.5%, e.g., about 0.005% to 0.01%, about 0.005% to 0.02%, about 0.005% to 0.03%, about 0.005% to 0.04%, about 0.005% to 0.05%, about 0.01% to 0.02%, about 0.01% to 0.03%, about 0.01% to 0.04%, about 0.01% to 0.05%, about 0.01% to 0.1%, about 0.1% to 0.2%, about 0.1% to 0.3%, about 0.1% to 0.4%, or about 0.1% to 0.5%. In some embodiments, the peach gum polysaccharides are present in the composition at a concentration of about 0.01% to 0.1%.

[0050] In some embodiments, the compositions provided herein include a Bacillus fermented probiotic. The Bacillus fermented postbiotic is believed to inhibit the growth of pathogenic microorganisms while simultaneously reducing sebum content and inflammation. The Bacillus fermented postbiotic may be present in the composition at a concentration of about 0.05% to 0.5%, e.g., about 0.05% to 0.1%, about 0.05% to 0.2%, about 0.05% to 0.3%, about 0.05% to 0.4%, about 0.1% to 0.2%, about 0.1% to 0.3%, about 0.1% to 0.4%, or about 0.1% to 0.5%. In some embodiments, the Bacillus fermented postbiotic is present in the composition at a concentration of about 0.1% to 0.3%.

[0051] In some embodiments, the compositions provided herein include Opuntia ficus-indica (prickly pear cactus) or an extract thereof. Opuntia ficus-indica or an extract thereof is believed to complement the microbiome regulator as an additional soothing and moisturizing component. The Opuntia ficus-indica extract can be a fruit extract, leaf extract, root extract, stem extract, seed extract, flower extract, or a combination thereof. In some embodiments, the Opuntia ficus-indica extract is a whole plant extract.

[0052] Opuntia ficus-indica or an extract thereof can be present in the composition at a concentration of about 0.005% to 0.5%, e.g., about 0.005% to 0.01%, about 0.005% to 0.02%, about 0.005% to 0.03%, about 0.005% to 0.04%, about 0.005% to 0.05%, about 0.01% to 0.02%, about 0.01% to 0.03%, about 0.01% to 0.04%, about 0.01% to 0.05%, about 0.01% to 0.1%, about 0.1% to 0.2%, about 0.1% to 0.3%, about 0.1% to 0.4%, or about 0.1% to 0.5%. In some embodiments, opuntia ficus-indica or an extract thereof is present in the composition at a concentration of about 0.01% to 0.1%.

[0053] Table 3: Exemplary compositions useful as scalp essences [Table 3]

[0054] In some embodiments, the compositions provided herein comprise about 1%-3% α-glucan oligosaccharides, about 0.1%-0.3% Bacillus ferment filtrate or extract thereof, about 0.1%-0.3% Opuntia ficus-indica or extract thereof, and about 0.01%-0.1% peach gum. In some embodiments, the compositions provided herein consist essentially of about 1%-3% α-glucan oligosaccharides, about 0.1%-0.3% Bacillus ferment filtrate or extract thereof, 0.1%-0.3% Opuntia ficus-indica or extract thereof, and about 0.01%-0.1% peach gum.

[0055] In some embodiments, the composition comprises about 0.1% to 0.3% by weight of sarcosine, about 0.01% to 0.1% by weight of kunzea pomifera or an extract thereof, about 0.01% to 0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% by weight of tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% by weight of pea (pisum sativum) or an extract thereof, and about 0.01% to 0.1% by weight of chia (salvia hispanica) or an extract thereof. In some embodiments, the composition comprises about 0.01%-0.1% by weight of α-glucan oligosaccharides, about 0.01%-0.1% by weight of kunzea pomifera or an extract thereof, about 0.01%-0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, and about 0.01%-0.1% by weight of Tasmannia lanceolata or an extract thereof. In some embodiments, the composition comprises about 1% to 3% by weight of α-glucan oligosaccharides, about 0.1% to 0.3% by weight of Bacillus ferment filtrate or an extract thereof, about 0.1% to 0.3% by weight of Opuntia ficus-indica or an extract thereof, and about 0.01% to 0.1% by weight of peach gum (Prunus persica gum).

[0056] In some embodiments, the composition further comprises about 1%-3% smithsonite or an extract thereof. In some embodiments, the composition provided herein consists essentially of about 1%-3% α-glucan oligosaccharides, about 0.1%-0.3% Bacillus ferment filtrate or an extract thereof, 0.1%-0.3% Opuntia ficus-indica or an extract thereof, about 0.01%-0.1% Prunus persica gum, and about 1%-3% Smithsonite or an extract thereof.

[0057] In some embodiments, the composition further comprises glycerin, propanediol, caprylyl / capryl glucoside, guar hydroxypropyltrimonium chloride, sodium benzoate, potassium sorbate, hydrolyzed quinoa, fragrance, lactic acid, zingiber officinale (ginger) or an extract thereof, tetrasodium glutamate diacetate, diisopropyl adipate, triethyl citrate, sodium hydroxide, and / or benzyl alcohol.

[0058] Glycerin, propanediol and / or smithsonite or extracts thereof may each be present in the composition at a concentration of about 0.5% to 5%, and in some embodiments, about 1% to 3%.

[0059] Caprylyl / capryl glucoside, guar hydroxypropyltrimonium chloride, and / or sodium benzoate may each be present in the composition at a concentration of about 0.1% to 1%, and in some embodiments at a concentration of about 0.3% to 0.99% (e.g., useful as a scalp essence).

[0060] The potassium sorbate, hydrolyzed quinoa, flavor, lactic acid, and / or Zingiber officinale or an extract thereof may each be present in the composition (e.g., useful as a scalp essence) at a concentration of about 0.05% to 0.5%, and in some embodiments, at a concentration of about 0.1% to 0.3%. The Zingiber officinale extract may be a fruit extract, a leaf extract, a root extract, or a combination thereof. In some embodiments, the Zingiber officinale extract is a whole plant extract.

[0061] Tetrasodium glutamic acid diacetate, diisopropyl adipate, triethyl citrate, sodium hydroxide, and / or benzyl alcohol may each be present in the composition at a concentration of about 0.05% to 0.5%, and in some embodiments, at a concentration of about 0.01% to 0.1%.

[0062] In some embodiments, the composition further comprises water, hi some embodiments, water is present in a concentration of at least about 30%, up to about 90%, up to about 95%, up to about 96%, up to about 97%, up to about 98%, or up to about 99%.

[0063] Excipients and Carriers In some embodiments, the compositions provided herein further comprise a cosmetically acceptable carrier or excipient. In some embodiments, the carrier is an alcohol-free carrier. In some embodiments, the carrier base fluid that may be used in the composition includes any carrier fluid or excipient combination suitable for use in cosmetic and / or pharmaceutical applications. For example, the composition may comprise an aqueous carrier base fluid.

[0064] In some embodiments, the carrier-based fluid may act as a solvent, carrier, diluent, and / or dispersant for the components of the composition, allowing the components to be applied evenly to the skin surface (e.g., topically applied) at an appropriate dilution. The carrier-based fluid may also facilitate penetration of the composition into the skin.

[0065] In some embodiments, the carrier-based fluid comprises a lotion suitable for topical application. In such embodiments, the lotion may comprise carbomer, water, glycerin, isopropyl myristate, mineral oil, stearic acid, glycol stearate, cetyl alcohol, dimethicone, preservatives, triethanolamine, and the like, or combinations thereof.

[0066] In some embodiments, the carrier-based fluid comprises a gel suitable for topical application, in such embodiments, the gel may comprise water, carbomer, glycerin, propylene glycol, preservatives, and the like, or combinations thereof.

[0067] Alternatively, the carrier base fluid may comprise the balance of the composition after taking into account the amounts of other ingredients used.

[0068] In another embodiment, one or more of the components of the composition can be solubilized with a solubilizing agent prior to mixing with the carrier-based fluid, such that the components are soluble in the carrier-based fluid. Non-limiting examples of solubilizing agents suitable for use in the present disclosure include water, glycerin (e.g., vegetable glycerin), various esters, polyethylene glycol (PEG), derivatives thereof, or combinations thereof.

[0069] In some embodiments, the composition may contain one or more inactive ingredients, such as one or more surfactants, cosolvents, and excipients or fillers (e.g., solids, semi-solids, liquids, etc.); emollients; delivery enhancers; circulation enhancers; antimicrobial agents; anti-inflammatory agents; foaming agents; carriers; diluents; binders (e.g., dextran); thickening agents; gelling agents; vitamins; retinoids and retinols (e.g., vitamin B3, vitamin A, etc.); pigments; fragrances; sunscreens and sunblocks; antioxidants and radical scavengers (e.g., tocopherol acetate or vitamin E acetate); organic hydroxy acids; exfoliants; skin conditioners (e.g., ethylhexylglycerin, hydrolyzed soy protein, glycol distearate, cyclopentasiloxane, quaternium-79 hydrolyzed keratin, propylene glycol, etc.); humectants (e.g., hydrolyzed soy protein, propylene glycol, etc.), and the like. glycols, etc.); ceramides, pseudoceramides; phospholipids, sphingolipids, cholesterol, glucosamine; pharma- ceutically acceptable penetrating agents (e.g. n-decylmethylsulfoxide, lecithin organogel, tyrosine, lysine, etc.); preservatives (e.g. phenoxyethanol, benzoic acid, dehydroacetic acid, polyaminopropyl biguanide, DMDM ​​hydantoin or 1,3-bis(hydroxymethyl)-5,5-dimethylimidazolidine-2,4-dione, iodopropynyl butylcarbamate, iodopropynyl butylcarbonate, stearalkonium chloride, etc.); amino acids such as praline; pyrrolidone carboxylic acid, its derivatives and salts; sugar isomers; panthenol (provitamin of vitamin B5); buffers in combination with bases such as triethanolamine or sodium hydroxide; waxes such as beeswax, ozokerite wax, paraffin wax, etc.;The composition may further comprise plant extracts obtained from plants such as aloe vera leaves, cornflower, witch hazel, elderflower, green tea (e.g., Camellia sinensis) leaves, grape (e.g., Vitis vinifera) seeds, jojoba (e.g., Simmondsia chinensis) seeds, tea plant (e.g., Malaleuca alternifolia) leaves, rosemary (e.g., Rosmarinus officinalis), henna, sunflower (e.g., Helianthus annuus) seeds, wild soybean (e.g., Glycine soja), argan tree kernels or argan, cucumber, shiso, or combinations thereof. As will be appreciated by one of ordinary skill in the art upon review of this disclosure, the selection and amounts of optional ingredients may vary.

[0070] In some embodiments, the compositions of the present invention further comprise sodium C14-16 olefin sulfonate, cetearyl alcohol, cocamidopropyl betaine, brassicamidopropyl dimethylamine, propanediol, sodium lauroyl sarcosinate, fragrance, sodium chloride, lactic acid, piroctone olamine, caprylyl / capryl glucoside, guar hydroxypropyltrimonium chloride, diisopropyl adipate, tetrasodium glutamate diacetate, glycol distearate, triethyl citrate, benzyl alcohol, sodium benzoate, potassium sorbate, cocamide MIPA, sodium hydroxide, hydroxypropyl methylcellulose, polyquatemium-37, isopropyl alcohol, citral, geraniol, caprylyl glyceryl ether, limonene, tetrasodium EDTA, linalool, citric acid, or CI 77289 (chromium hydroxide green).

[0071] In some embodiments, the compositions provided herein may contain any of a variety of ingredients selected from the group consisting of water, glycerin, Camellia sinensis (green tea) leaf or extract thereof, glycine, ramixus europaea wood or extract thereof, sodium metabisulfite, zinc chloride, pisum sativum (pea) sprouts or extract thereof, alcohol, olea europaea (olive) leaf or extract thereof, curcuma longa (turmeric) root or extract thereof, equisetum arvense (horseback) or extract thereof, hippophae rhamnoides (seabuckthorn) fruit oil, laminaria saccharina (Neptune's kelp) or extract thereof, lepidium meyenii (sea buckthorn) fruit oil, olea europaea (olive) leaf or extract thereof, curcuma longa (turmeric) root or extract thereof, equisetum arvense (horseback) or extract thereof, hippophae rhamnoides (sea buckthorn) fruit oil, laminaria saccharina (Neptune's kelp) or extract thereof, lepidium meyenii (sea buckthorn) fruit oil, olea europaea (olive) leaf or extract thereof ... meyenii (maca) root or extract thereof, melaleuca altemifolia (tea plant) leaf oil, moringa oleifera (moringa) leaf or extract thereof, panax ginseng (ginseng) root or extract thereof, DL-panthenol, L-theanine, melatonin, niacinamide, sodium dehydroacetate, sodium hyaluronate, and one or more of phytic acid.

[0072] It will be apparent to one of ordinary skill in the art that, in addition to the exemplary compositions above, other compositions can be made or produced using the components listed in Table 1, Table 2, and / or Table 3.

[0073] In some embodiments, the compositions described herein are sulfate-free. In some embodiments, the compositions described herein are paraben-free. In some embodiments, the compositions described herein are silicone-free. In some embodiments, the compositions described herein are phthalate-free. In some embodiments, the compositions described herein are synthetic fragrance-free. In some embodiments, the compositions described herein are synthetic hormone-free.

[0074] In some embodiments, the compositions described herein are essentially free of sulfates. In some embodiments, the compositions described herein are essentially free of parabens. In some embodiments, the compositions described herein are essentially free of silicones. In some embodiments, the compositions described herein are essentially free of phthalates. In some embodiments, the compositions described herein are essentially free of synthetic fragrances. In some embodiments, the compositions described herein are essentially free of synthetic hormones. As used herein, "essentially free" of a particular ingredient means that the composition contains about 1% or less by weight of the ingredient.

[0075] How to use Also provided herein is a method for improving hair health or scalp health or treating or preventing hair loss using the compositions described herein. An example of hair health includes hair quality. An example of hair loss treatment includes reducing or inhibiting hair loss. Thus, further provided is a method for improving hair health or scalp health or treating or preventing hair loss in a subject in need of such treatment, comprising applying an effective amount of the compositions disclosed herein to the scalp of the subject.

[0076] In one aspect, provided herein is a method of improving hair health in a subject in need thereof, comprising applying to the scalp of the subject an effective amount of a composition described herein.

[0077] situation In addition to improving hair health or scalp health or treating or preventing hair loss, the methods and compositions described herein may also improve the appearance of the hair on the scalp to which the compositions are applied, for example, by thickening the hair and improving the shine, condition and manageability of the hair.

[0078] Administration The compositions described herein can be applied to the hair or scalp of a subject using any suitable method of administration. The compositions described herein can be applied at least once a week, for example at least every two days, or at least once every day. For example, application can be twice a day. In general, application of the compositions described herein can be continued indefinitely. Alternatively, application can be repeated for only a limited period of time, such as a few weeks or months. Application can then be repeated at a later date for a similar period of time.

[0079] Most commonly, the area of ​​skin to which the compositions described herein are applied is the scalp.

[0080] In some embodiments, a method for treating or preventing hair loss comprises topically applying a composition described herein to the scalp or other body area where hair loss treatment or prevention is desired. The compositions described herein may be useful in a wide variety of finished products, including pharmaceuticals and cosmetics. The compositions described herein may be prepared, packaged, and labeled to treat or prevent hair loss or to attenuate the hair loss process.

[0081] In some embodiments, the compositions described herein are administered topically, for example, to the scalp of a subject, in the form of a solution, gel, lotion, cream, ointment, oil-in-water emulsion, water-in-oil emulsion, stick, spray, aerosol, paste, mousse, tonic, shampoo, liposome, or other cosmetically and topically suitable form. In some embodiments, the compositions described herein are applied topically to the area to be treated, such as the scalp of a human, by spraying, tapping, wiping, rubbing, or a combination thereof.

[0082] In some embodiments, the compositions described herein are applied topically in the form of scalp irritant foam. In another embodiment, the compositions described herein are dispersed topically on the scalp in an aerosol form, such as a chlorofluorocarbon solvent, for delivery in a spray form. The spray form may provide several advantages, such as high payload, improved uptake, ease of application, and reduced hair tangling in the application area. In such embodiments, the compositions described herein may remain on the scalp for about 1 week, alternatively about 1 day, alternatively about 12 hours, alternatively about 4 hours, alternatively about 1 hour, alternatively about 30 minutes, alternatively about 5 minutes, or alternatively about 1 minute. The compositions described herein may be removed at any desired time by washing and / or rinsing the scalp. In some embodiments, the compositions are applied daily after application without rinsing until the next regular hair wash. In some embodiments, the compositions may be allowed to remain on the scalp for at least 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, or 48 hours.

[0083] In some embodiments, compositions useful, for example, as scalp cleansing agents, are applied topically to the scalp of a subject and then rinsed from the subject's scalp using an aqueous rinse, such as water.

[0084] Accordingly, further provided herein is a method of improving hair health or scalp health, or treating or preventing hair loss, in a subject in need thereof, comprising applying an effective amount of a composition disclosed herein to the scalp of the subject, and then rinsing the composition from the subject's scalp.

[0085] In some embodiments, compositions useful, for example, as a scalp mask, are applied topically to the scalp of a subject and allowed to remain on the scalp for at least about 5 to about 8 minutes, and in some embodiments, for at least about 5 to about 10 minutes, and then rinsed from the subject's scalp using an aqueous rinse, such as, for example, water.

[0086] Accordingly, further provided herein is a method of improving hair health or scalp health, or treating or preventing hair loss, in a subject in need thereof, comprising applying an effective amount of a composition disclosed herein to the scalp of the subject, allowing the composition to remain on the subject's scalp for at least about 5 to about 10 minutes, and thereafter rinsing the composition from the subject's scalp.

[0087] In some embodiments, compositions useful, for example, as scalp essences, are applied topically to the scalp of a subject and allowed to remain on the scalp for at least about 6 hours, in some embodiments, at least about 12 hours, in some embodiments, at least about 24 hours, and in some embodiments, at least about 48 hours, and then rinsed from the subject's scalp using an aqueous rinse, such as, for example, water.

[0088] Accordingly, further provided herein is a method of improving hair health or scalp health, or treating or preventing hair loss, in a subject in need thereof, comprising applying an effective amount of a composition disclosed herein to the scalp of the subject, allowing the composition to remain on the subject's scalp for at least about 6 to about 48 hours, and thereafter rinsing the composition from the subject's scalp.

[0089] In some embodiments, the composition is topically administered at least daily, and in some embodiments, twice daily, for a period of time. For example, a user can topically administer the composition directly to the hair loss site or other area where hair loss treatment or prevention is desired by gently massaging the composition of the present disclosure into the desired area. This process can be repeated later on the same day. In some embodiments, the composition can be left on the scalp or other area where hair loss treatment or prevention is desired between administrations on the same or different days. As a person skilled in the art will understand with the aid of this disclosure, the composition can be topically applied / administered on a regular basis on a daily basis. Generally, the composition is topically administered daily, although more frequent application can also be used.

[0090] In some embodiments, application of the composition can continue for any suitable period of time.For example, within a few weeks to a few months of first application, the user may notice a reduction in hair loss.It should be understood that the frequency of application of the composition may vary depending on the desired effect.In particular, it is believed that the degree of cosmetic enhancement may vary directly depending on the total amount and combination of ingredients of the composition.

[0091] As will be appreciated by those of skill in the art with the benefit of this disclosure, other methods can be used to topically apply / administer the compositions described herein.

[0092] In some embodiments, the compositions described herein can be advantageously used to treat or prevent hair loss. For example, as disclosed herein, the compositions described herein can reduce and / or stop hair loss within a period of about 7 to about 80 days, or about 10 to about 28 days, or about 14 to about 21 days.

[0093] Without intending to be limited by theory, it is believed that the compositions described herein may advantageously treat or prevent hair loss for a period of from about 4 weeks to about 20 weeks, alternatively from about 4 weeks to about 16 weeks, alternatively from about 4 weeks to about 10 weeks.

[0094] The dosage of the compositions described herein may depend on many factors, including, but not limited to, the severity of hair loss, the subject's age, general health, and the individual's response to the composition. Thus, the dosage of the compositions described herein may vary and be easily adjusted depending on the response of each individual subject.

[0095] The compositions described herein can be administered topically, transdermally, or subcutaneously. Those skilled in the art will recognize the advantages of certain routes of administration. In some embodiments, the compositions are administered by subcutaneous injection.

[0096] Dosage forms for the topical or transdermal administration of compositions described herein include powders, sprays, ointments, pastes, creams, lotions, gels, solutions, patches and inhalants.

[0097] The compositions described herein can be freely combined with each other. Thus, in some embodiments, a composition useful as a scalp mask is administered in combination with a composition useful as a scalp cleanser. In some embodiments, a composition useful as a scalp mask is administered in combination with a composition useful as a scalp essence. In some embodiments, a composition useful as a scalp cleanser is administered in combination with a composition useful as a scalp essence. In some embodiments, a composition useful as a scalp mask, a composition useful as a scalp cleanser, and a composition useful as a scalp essence are administered in combination. When used in combination, the compositions can be administered sequentially, concurrently, or simultaneously. When used sequentially, the compositions can be administered in any suitable order.

[0098] In some embodiments, the methods provided herein further comprise microneedling the scalp of the subject. In some embodiments, the methods further comprise microneedling the scalp of the subject prior to applying a composition described herein to the scalp of the subject.

[0099] In some embodiments, the methods provided herein include administering to the scalp of a subject between about 0.1% to 0.3% by weight of sarcosine, about 0.01% to 0.1% by weight of kunzea pomifera or an extract thereof, about 0.01% to 0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% by weight of tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% by weight of pea (pisum sativum) or an extract thereof, about 0.01% to 0.1% by weight of chia (salvia hispanica), or about 0.01% to 0.1% by weight of sarcosine, about 0.01% to 0.1% by weight of kunzea pomifera or an extract thereof, about 0.01% to 0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% by weight of tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% by weight of pea (pisum sativum) or an extract thereof, about 0.01% to 0.1% by weight of chia (salvia hispanica) or extract thereof, about 0.1% to 0.3% by weight of vaccinium myrtillus) or extract thereof, about 0.1% to 0.3% by weight of saccharum officinarum (sugar cane) or extract thereof, about 0.01% to 0.1% by weight of citrus aurantium dulcis (orange) or extract thereof, about 0.01% to 0.1% by weight of citrus limon (lemon) or extract thereof, about 0.01% to 0.1% by weight of acer saccharum (acer saccharum (sugar maple) or extract thereof and about 1% to 3% by weight of jojoba esters, Simmondsia chinensis (Simmondsia chinensis) or extract thereof.In some embodiments, the method further comprises administering to the subject's scalp about 30% by weight water, about 3% to 10% by weight glycerin, about 1% to 3% by weight cetearyl alcohol, about 1% to 3% by weight brassicamidopropyl dimethylamine, about 1% to 3% by weight fragrance, about 0.3% to 0.99% by weight polyquatemium-37, about 0.3% to 0.99% by weight lactic acid, about 0.3% to 0.99% by weight sodium benzoate, about 0.1% to 0.3% by weight piroctone olamine, about 0.1% to 0.3% by weight potassium sorbate, about 0.01% to 0.1% by weight tetrasodium glutamate diacetate, about 0.01% to 0.1% by weight montmorillonite, about 0.01% to 0.1% by weight CI 62811, and about 0.01% to 0.1% by weight CI 62811. 77289 (chromium hydroxide green), about 0.01% to 0.1% by weight isopropyl alcohol, about 0.01% to 0.1% by weight sodium hydroxide, about 0.01% to 0.1% by weight tetrasodium EDTA, and / or about 0.01% to 0.1% by weight Aspergillus ferment.

[0100] In some embodiments, the methods provided herein include applying to the scalp of a subject a kunzea pomifera extract, a ryeberry (syzygium luehmannii) extract, a tasmannia lanceolata extract, a pea (pisum sativum) extract, a chia (salvia hispanica) extract, a sugar cane (saccharum officinarum) extract, a citrus aurantium dulcis extract, a citrus limon extract, and / or an acer saccharum extract, pomifera extract, ryeberry (syzygium luehmannii) extract, tasmannia lanceolata extract, pea (pisum sativum) extract, chia (salvia hispanica) extract, sugar cane (saccharum officinarum) extract, citrus aurantium dulcis extract, citrus limon extract, and acer saccharum extract may be fruit extracts, leaf extracts, root extracts, or combinations thereof.In some embodiments, the method includes applying to the scalp of the subject a kunzea pomifera extract, a ryeberry (syzygium luehmannii) extract, a tasmannia lanceolata extract, a pea (pisum sativum) extract, a chia (salvia hispanica) extract, a sugar cane (saccharum officinarum) extract, a citrus aurantium dulcis extract, a citrus limon extract, or an acer saccharum extract, wherein the kunzea pomifera extract, the ryeberry (syzygium luehmannii) extract, the tasmannia lanceolata extract, the pea (pisum sativum) extract, the chia (salvia hispanica) extract, the sugar cane (saccharum officinarum) extract, the citrus aurantium dulcis extract, the citrus limon extract, or the acer saccharum extract. luehmannii) extract, Tasmannia lanceolata extract, pea (pisum sativum) extract, chia (salvia hispanica) extract, sugar cane (saccharum officinarum) extract, citrus aurantium dulcis extract, citrus limon extract, and acer saccharum extract are whole plant extracts.

[0101] In some embodiments, the methods provided herein further comprise rinsing the subject's scalp at least about 5 minutes to about 10 minutes after application.

[0102] In some embodiments, (a) the application is twice weekly application. In some embodiments, the application is the first application, (b) the method improves scalp hydration by at least 10% within about 1 month, about 2 months, or about 3 months after the first application; (c) the improvement is measured using a corneometer. In some embodiments, the application is the first application, (b) the method reduces scalp sebum production by at least 10% within about 1 month, about 2 months, or about 3 months after the first application, (c) the reduction is measured using a sebometer.

[0103] In some embodiments, the methods provided herein include applying to the scalp of a subject about 0.01% to 0.1% by weight of α-glucan oligosaccharides, about 0.01% to 0.1% by weight of kunzea pomifera or an extract thereof, about 0.01% to 0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% by weight of Tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% by weight of hydrolyzed quinoa, and about 0.01% to 0.1% by weight of Aspergillus fermentate. In some embodiments, the method further comprises administering to the scalp of the subject about 30% by weight water, about 3% to 10% by weight sodium C14-16 olefin sulfonate, about 3% to 10% by weight cocamidopropyl betaine, about 3% to 10% by weight sodium lauroyl sarcosinate, about 1% to 3% by weight propanediol, about 1% to 3% by weight sodium chloride, about 0.3% to 0.99% by weight fragrance, about 0.3% to 0.99% by weight cocamide MIPA, about 0.3% to 0.99% by weight glycol distearate, about 0.3% to 0.99% by weight citric acid, about 0.3% to 0.99% by weight hydroxypropyl methylcellulose, about 0.3% to 0.99% by weight guar hydroxypropyltrimonium chloride, about 0.3% to 0.99% by weight glycerin ... 99% by weight glycerin, about 0.3% to 0.99% by weight sodium benzoate, about 0.3% to 0.99% by weight polyquatemium-73, about 0.1% to 0.3% by weight caprylyl / capryl glucoside, about 0.1% to 0.3% by weight potassium sorbate, about 0.1% to 0.3% by weight glycolipids, about 0.01% to 0.1% by weight % tetrasodium glutamic acid diacetate, about 0.01% to 0.1% diisopropyl adipate, about 0.01% to 0.1% sodium hydroxide, about 0.01% to 0.1% triethyl citrate, about 0.01% to 0.1% caprylyl glyceryl ether, and / or about 0.01% to 0.1% benzyl alcohol.

[0104] In some embodiments, the methods provided herein include applying to the scalp of a subject a kunzea pomifera extract, a ryeberry (syzygium luehmannii) extract, and / or a tasmannia lanceolata extract, wherein the kunzea pomifera extract, the ryeberry (syzygium luehmannii) extract, and / or the tasmannia lanceolata extract is a fruit extract, a leaf extract, a root extract, or a combination thereof. In some embodiments, the methods provided herein include applying to the scalp of a subject a kunzea pomifera extract, a ryeberry (syzygium luehmannii) extract, and / or a tasmannia lanceolata extract, wherein the kunzea pomifera extract, the ryeberry (syzygium luehmannii) extract, and / or the tasmannia lanceolata extract are whole plant extracts.

[0105] In some embodiments, the method further comprises rinsing the scalp of the subject. In some embodiments, the application is a once-daily application.

[0106] In some embodiments, (a) the application is a first application, (b) the method results in at least a 10% improvement in scalp hydration within about 1 month, about 2 months, or about 3 months after the first application, and (c) the improvement is measured using a corneometer. In some embodiments, (a) the application is a first application, (b) the method results in at least a 10% reduction in scalp sebum production within about 1 month, about 2 months, or about 3 months after the first application, and (c) the reduction is measured using a sebometer.

[0107] In some embodiments, the methods provided herein include applying to the scalp of a subject about 1% to 3% by weight of α-glucan oligosaccharides, about 0.1% to 0.3% by weight of Bacillus ferment filtrate or an extract thereof, about 0.1% to 0.3% by weight of Opuntia ficus-indica or an extract thereof, about 0.01% to 0.1% by weight of Prunus persica gum, and about 1% to 3% by weight of Smithsonite or an extract thereof. In some embodiments, the method further comprises administering to the subject's scalp about 30% by weight of water, about 1%-3% by weight of glycerin, about 1%-3% by weight of propanediol, about 0.3%-0.99% by weight of caprylyl / capryl glucoside, about 0.3%-0.99% by weight of guar hydroxypropyltrimonium chloride, about 0.3%-0.99% by weight of sodium benzoate, about 0.1%-0.3% by weight of potassium sorbate, about 0.1%-0.3% by weight of hydrolyzed quinoa, about 0.1%-0.3% by weight of fragrance, about 0.1%-0.3% by weight of lactic acid, about 0.1%-0.3% by weight of Zingiber officinale. officinale (ginger) or an extract thereof, about 0.01% to 0.1% by weight of tetrasodium glutamate diacetate, about 0.01% to 0.1% by weight of diisopropyl adipate, about 0.01% to 0.1% by weight of triethyl citrate, about 0.01% to 0.1% by weight of sodium hydroxide, or about 0.01% to 0.1% by weight of benzyl alcohol.

[0108] In some embodiments, the methods provided herein include applying an opuntia ficus-indica extract and / or a zingiber officinale extract to the scalp of a subject, where the opuntia ficus-indica extract and / or the zingiber officinale extract is a fruit extract, a leaf extract, a root extract, or a combination thereof. In some embodiments, the methods provided herein include applying an opuntia ficus-indica extract and / or a zingiber officinale extract to the scalp of a subject, where the opuntia ficus-indica extract and / or the zingiber officinale extract is a whole plant extract.

[0109] In some embodiments, the application occurs daily. In some embodiments, the method further comprises washing the subject's scalp at least 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, or 48 hours after application.

[0110] In some embodiments, (a) the application is a first application, (b) the method results in at least a 10% improvement in scalp hydration within about one month, about two months, or about three months after the first application, and (c) the improvement is measured using a corneometer. In some embodiments, (a) the application is a first application, (b) the method results in at least a 10% reduction in scalp sebum production within about one month, about two months, or about three months after the first application, and (c) the reduction is measured using a sebometer.

[0111] In some embodiments, the methods provided herein include applying to the scalp of a subject sodium C14-16 olefin sulfonate, cetearyl alcohol, cocamidopropyl betaine, brassicamidopropyl dimethylamine, propanediol, sodium lauroyl sarcosinate, fragrance, sodium chloride, lactic acid, piroctone olamine, caprylyl / capryl glucoside, guar hydroxypropyltrimonium chloride, diisopropyl adipate, tetrasodium glutamate diacetate, glycol distearate, triethyl citrate, benzyl alcohol, sodium benzoate, potassium sorbate, cocamide MIPA, sodium hydroxide, hydroxypropyl methylcellulose, polyquatemium-37, isopropyl alcohol, citral, geraniol, caprylyl glyceryl ether, limonene, tetrasodium EDTA, linalool, citric acid, or CI 77289 (chromium hydroxide green).

[0112] In some embodiments, the methods described herein do not include applying sulfates to the scalp of the subject. In some embodiments, the methods described herein do not include applying parabens to the scalp of the subject. In some embodiments, the methods described herein do not include applying silicones to the scalp of the subject. In some embodiments, the methods described herein do not include applying phthalates to the scalp of the subject. In some embodiments, the methods described herein do not include applying synthetic fragrances to the scalp of the subject. In some embodiments, the methods described herein do not include applying synthetic hormones to the scalp of the subject.

[0113] Treatment or prevention of hair loss The compositions described herein can be used to treat or prevent hair loss in any situation in which such treatment or prevention is desired.

[0114] In some embodiments, the subject treated according to the methods described herein is experiencing hair loss associated with one or more conditions, such as, but not limited to, anagen effluvium, telogen effluvium, drug-related alopecia, radiation therapy, poisoning, alopecia areata diffuse, alopecia areata, slow anagen syndrome, post-operative occipital alopecia, syphilis, traction alopecia (traction alopecia), premature male pattern baldness, alopecia areata, trichotillomania, tinea capitis, telogen effluvium, telogen pregnancy, chronic telogen effluvium, premature male pattern baldness, iron deficiency, malnutrition / dyspepsia, hypothyroidism, hyperthyroidism, systemic lupus erythematosus, chronic renal failure, liver dysfunction, progressive malignancy, viral or bacterial infection, and androgenetic alopecia.

[0115] In some embodiments, the hair loss treated or prevented by the methods described herein is associated with one or more conditions, such as, but not limited to, anagen effluvium, telogen effluvium, drug-related alopecia, radiation therapy, poisoning, alopecia areata diffuse, alopecia areata, slow anagen syndrome, post-operative occipital alopecia, syphilis, traction alopecia (traction alopecia), premature male pattern baldness, alopecia areata, trichotillomania, tinea capitis, telogen effluvium, telogen pregnancy, chronic telogen effluvium, premature male pattern baldness, iron deficiency, malnutrition / dyspepsia, hypothyroidism, hyperthyroidism, systemic lupus erythematosus, chronic renal failure, liver dysfunction, progressive malignancy, viral or bacterial infection, and androgenetic alopecia.

[0116] In particular, the method of the present disclosure is useful for treating or preventing androgenetic alopecia, alopecia areata, alopecia in drug-induced alopecia (e.g., after cancer chemotherapy), and reversal of alopecia caused by radiation therapy.In some embodiments, the condition is an age-related condition, such as age-related alopecia.In some embodiments, the condition (e.g., alopecia) is caused by malnutrition, mineral deficiency, and / or vitamin deficiency.

[0117] In some embodiments, the compositions described herein are useful for treating or preventing alopecia in humans. However, the compositions described herein are also useful for treating or preventing alopecia in non-human mammals. For example, the compositions described herein can be used in livestock animals, such as sheep, where treating or preventing fur (hair) loss would be of economic interest. The compositions can also be used to treat or prevent alopecia in pet animals, such as dogs, cats, and gerbils. Dosages useful to achieve this effect are consistent with the guidelines described herein. Similarly, the compositions described herein can be administered using formulations typically used for veterinary use, taking into account the type of animal being treated. Other uses of the compositions described herein for treating or preventing alopecia will be readily apparent to those of skill in the art based on the disclosure of this application.

[0118] In one embodiment, the compositions described herein can advantageously treat or prevent hair loss from dormant and / or damaged hair follicles, e.g., the compositions described herein can have a rejuvenating effect on hair follicles.

[0119] How to assess efficacy Methods for evaluating the effectiveness of the methods and compositions described herein for treating or preventing hair loss are known in the art and described herein.

[0120] A simple method for assessing the improvement of hair health or scalp health, or the treatment or prevention of hair loss, is to take a picture of a test area of ​​skin before and after applying a composition as described herein. The skin, e.g., scalp, may be shaved for this purpose. A picture is taken. A composition as described herein is then applied. A second picture is then taken. The improvement of hair health or scalp health, or the treatment or prevention of hair loss, can be quantified by counting any combination of (a) the number of ingrown hairs, (b) the length of ingrown hairs, (c) the thickness of ingrown hairs, (d) the straightness of ingrown hairs, or (e) the area of ​​hair growth.

[0121] For example, an individual can be evaluated for improvement of hair health or scalp health, or treatment or prevention of hair loss. An individual administered the compositions described herein can show at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 100% or more reduction in hair loss as measured by any of the parameters described above. This can be compared to hair loss in an individual not administered the composition. Reduction in hair loss can be evaluated by the number or thickness of hair. Alternatively, it can be evaluated by measuring the area of ​​hair loss.

[0122] Clinical Scale Any suitable clinical scale for measuring or assessing hair health, scalp health, or hair loss known in the art or described herein can be used to measure the effectiveness of the methods described herein. For examples of scales that can be used to assess the effectiveness of the methods described herein, see Gupta and Mysorel, J Cutan Aesthet Surg. 2016 Jan-Mar;9(1):3-12, which is incorporated herein by reference in its entirety.

[0123] For example, in some embodiments, the Hamilton-Norwood scale can be used to measure the effectiveness of the methods described herein. The Hamilton-Norwood scale categorizes the severity of male pattern baldness into seven stages. It is widely used to evaluate alopecia, but some problems with reproducibility have been reported (Guarrera et al, Int J Trichology, 2009 Jul-Dec;1(2):120-122). The mildest stage is stage 1, where there is no significant hair loss and no visible receding hair. In stage 2, hair loss becomes visible around the temples, but is usually not yet significant. In stage 3, hair loss becomes more noticeable, with a receding hairline, which may appear in a V-, U-, or M-shaped pattern. Hair loss on the crown of the head may also occur in stage 3, but is more noticeable in stage 4. In stage 5, the remaining hairs become significantly thinner and reced further, and in stage 6, the bald areas connect. The most severe stage, stage 7, usually results in only fine or thin bands of hair remaining around the sides and back of the head.

[0124] In some embodiments, the methods described herein result in the treatment or prevention of alopecia as measured by the Hamilton-Norwood scale. In some embodiments, the methods result in an improvement within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 5 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of a composition described herein, as measured by the Hamilton-Norwood scale.

[0125] In some embodiments, the treatment methods described herein result in a slowing of hair loss as measured by the Hamilton-Norwood scale, e.g., a slowing of hair loss compared to the rate of hair loss before treatment. In some embodiments, the subject's score on the Hamilton-Norwood scale is maintained for at least about 3 months, at least about 4 months, at least about 5 months, at least about 6 months, at least about 7 months, at least about 8 months, at least about 9 months, at least about 10 months, at least about 11 months, at least about 12 months, at least about 15 months, at least about 18 months, at least about 2 years, at least about 3 years, at least about 4 years, or at least about 5 years after the first administration of the compositions described herein. In some embodiments, the subject has previously experienced a decrease in score on the Hamilton-Norwood scale.

[0126] Alternatively or additionally, the effectiveness of the methods described herein can be measured using the Ludwig scale.The Ludwig scale is commonly used to classify the severity of hair loss in women into three stages (Ludwig, British Journal of Dermatology (1977) 97, 247).The mildest stage is stage 1, which may show thinning hair on the top of the head.Then, hair loss progresses through stage 2, where the scalp becomes visible, and in stage 3, the hair on the top of the head may fall out entirely.

[0127] In some embodiments, the methods described herein result in the treatment or prevention of hair loss as measured on the Ludwig scale. In some embodiments, the methods result in an improvement within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months of the first administration of a composition described herein as measured on the Ludwig scale.

[0128] In some embodiments, the treatment methods described herein slow down the progression of hair loss measured by the Ludwig scale.In some embodiments, the subject's score on the Ludwig scale is maintained at least about 3 months, at least about 4 months, at least about 5 months, at least about 6 months, at least about 7 months, at least about 8 months, at least about 9 months, at least about 10 months, at least about 11 months, at least about 12 months, at least about 15 months, at least about 18 months, at least about 2 years, at least about 3 years, at least about 4 years, or at least about 5 years after the first administration of the compositions described herein.

[0129] Hair Pull Test A hair pull test can be used to measure the effectiveness of the methods described herein. To perform the hair pull test, an evaluator holds a bundle of about 50-60 hairs close to the scalp and pulls the bundle firmly by slowly sliding their fingers along the hair shaft, avoiding pulling with too much force or too fast. The pulled hairs are then counted and any broken hairs that fall out are discarded. This test is generally performed on multiple areas of the scalp, such as the vertex, parietal area, and / or occipital area. If more than 10% of the hairs in each bundle fall out of the scalp area, the hair pull test is considered positive, whereas if less than 10% of the hairs fall out, it is generally considered normal hair loss. See McDonald et al., 2017, J Am Acad. Dermatology;76(3):472-477. In some embodiments, the methods described herein result in the treatment or prevention of hair loss as measured by the hair pull test. In some embodiments, the method provides at least a 10% improvement as measured by a hair pull test within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of a composition described herein. In some embodiments, the method provides at least a 25% improvement as measured by a hair pull test within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of a composition described herein. In some embodiments, the methods provide at least a 50% improvement as measured by a hair pull test within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the initial administration of a composition described herein.

[0130] In some embodiments, the methods described herein provide hair loss retardation as measured by hair pull test.In some embodiments, the hair pull test score of the subject is maintained for at least about 3 months, at least about 4 months, at least about 5 months, at least about 6 months, at least about 7 months, at least about 8 months, at least about 9 months, at least about 10 months, at least about 11 months, at least about 12 months, at least about 15 months, at least about 18 months, at least about 2 years, at least about 3 years, at least about 4 years, or at least about 5 years after first administration of the compositions described herein.

[0131] Hair Tag Test The hair tag test can be used to measure the effectiveness of the methods described herein. The hair tag test is similar to the hair pull test, but also evaluates hair stability and hair loss. In the hair tag test, a hair is grasped near the root with the thumb and index finger, and the other hand is used to tug forcefully on the distal end of the same hair. The hair tag test is positive if the hair breaks, indicating fragility of the hair shaft (Xu et al. Frontiers in Medicine, 24 Jul 2017, 4:112).

[0132] In some embodiments, the methods described herein provide treatment or prevention of hair loss or hair damage as measured by hair tag test. In some embodiments, the methods provide at least 10% improvement as measured by hair tag test within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months from the first administration of the composition described herein. In some embodiments, the methods provide at least 25% improvement as measured by hair tag test within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months from the first administration of the composition described herein. In some embodiments, the methods result in at least a 50% improvement as measured by a hair tag test within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of a composition described herein.

[0133] In some embodiments, the methods of treatment described herein result in a delay in hair loss or damage as measured by a hair tag test. In some embodiments, the subject's hair tag test score is maintained for at least about 3 months, at least about 4 months, at least about 5 months, at least about 6 months, at least about 7 months, at least about 8 months, at least about 9 months, at least about 10 months, at least about 11 months, at least about 12 months, at least about 15 months, at least about 18 months, at least about 2 years, at least about 3 years, at least about 4 years, or at least about 5 years from the initial administration of a composition described herein.

[0134] Card Testing The card test can be used to measure the effectiveness of the methods described herein. The card test is a simple method of counting hair strands by placing a felt-covered card of a contrasting color to the subject's hair against the scalp and counting the number of hair strands that grow. It can also be used to determine hair thickness and health.

[0135] In some embodiments, the methods described herein provide treatment or prevention of hair loss as measured by card test. In some embodiments, the methods provide at least 10% improvement as measured by card test within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months from the first administration of the compositions described herein. In some embodiments, the methods provide at least 25% improvement as measured by card test within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months from the first administration of the compositions described herein. In some embodiments, the methods result in at least a 50% improvement as measured by the card test within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of a composition described herein.

[0136] In some embodiments, the method described herein provides the delay of hair loss measured by card test.In some embodiments, the number of new hair strands measured by card test is maintained for at least about 3 months, at least about 4 months, at least about 5 months, at least about 6 months, at least about 7 months, at least about 8 months, at least about 9 months, at least about 10 months, at least about 11 months, at least about 12 months, at least about 15 months, at least about 18 months, at least about 2 years, at least about 3 years, at least about 4 years, or at least about 5 years after first administration of the composition described herein.

[0137] Trichometry Trichometry can be used to measure the effectiveness of the methods described herein. Versions of tricometry include cross-sectional tricometry and using scalp and hair photography. The tricometry index is a measure of hair growth and shedding over 30 μm in diameter. A strand of hair taken from a 2x2 cm scalp area is placed in the cross-sectional tricometer to measure the cross-sectional area of ​​the strand. The tricometry index is then displayed, which is the average cross-sectional area of ​​the hair strand per cm of scalp surface. 2 Cross-sectional area of ​​the bundle per unit (unit: mm 2 ) multiplied by 100. See Wikramanayake et al., Int J Trichology, 2012 Oct-Dec;4(4):259-264. This method can also be performed digitally using a Folliscope® or videodermatoscope, which takes pictures of the scalp and hairs and provides information on hair coverage, such as the total number of hairs on the scalp and the diameter of each hair bundle. The images obtained can be graded, for example, according to the following scale:

[0138] [Table 4]

[0139] In some embodiments, the methods described herein provide for the treatment or prevention of hair loss as measured by the tricometry index measured by a cross-sectional tricometer, Folliscope®, or video dermatoscope.In some embodiments, the methods provide for at least 10% improvement in the tricometry index measured by a cross-sectional tricometer, Folliscope®, or video dermatoscope within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of the compositions described herein. In some embodiments, the method provides an improvement in tricometry index of at least 25% as determined by a cross-sectional tricometer, Folliscope®, or videodermatoscope within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of a composition described herein. In some embodiments, the method provides an improvement in tricometry index of at least 50% as determined by a cross-sectional tricometer, Folliscope®, or videodermatoscope within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of a composition described herein.

[0140] In some embodiments, the methods described herein provide for the delay of hair loss measured by cross-sectional trichometer.In some embodiments, the trichometric index of the subject measured by cross-sectional trichometer, Folliscope® or videodermatoscope is maintained for at least about 3 months, at least about 4 months, at least about 5 months, at least about 6 months, at least about 7 months, at least about 8 months, at least about 9 months, at least about 10 months, at least about 11 months, at least about 12 months, at least about 15 months, at least about 18 months, at least about 2 years, at least about 3 years, at least about 4 years, or at least about 5 years after the first administration of the compositions described herein.

[0141] Hair loss score The hair loss score determined by the hair loss scale can be used to measure the effectiveness of the methods described herein. The hair loss scale aims to distinguish between normal hair loss and hair loss. The hair loss scale is a visual scale showing nine hair piles of different lengths (shoulder, mid-back, lower back), each pile containing an increasing amount of hair (10, 50, 100, 200, 300, 400, 500, 600, and 700 hair bundles). The subject is asked to estimate the amount of hair loss after brushing. A score of 1-4 is considered normal hair loss, and a score of 5-9 is considered excessive hair loss. See Martinez-Velasco et al., Dermatol Tuer(Heidelb), 2017 Mar;7(1):155-165. Alternatively, the subject's hair is combed onto a white paper and a predetermined number of strokes (e.g., 2, 3, or 4) are made on each quarter of the hair. The hairs that fall onto the white surface are counted and the numbers compared over time.

[0142] In some embodiments, the methods described herein provide treatment or prevention of hair loss as measured by hair loss score on hair loss scale.In some embodiments, the methods provide at least 10% improvement in hair loss score on hair loss scale within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of the compositions described herein.In some embodiments, the methods provide at least 25% improvement in hair loss score on hair loss scale within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of the compositions described herein. In some embodiments, the methods result in an improvement in hair loss score of at least 50% as measured by the hair loss scale within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the initial administration of a composition described herein.

[0143] In some embodiments, the method described herein provides hair loss delay as measured by hair loss score measured by hair loss scale.In some embodiments, the hair loss score measured by the subject's hair loss scale is maintained for at least about 3 months, at least about 4 months, at least about 5 months, at least about 6 months, at least about 7 months, at least about 8 months, at least about 9 months, at least about 10 months, at least about 11 months, at least about 12 months, at least about 15 months, at least about 18 months, at least about 2 years, at least about 3 years, at least about 4 years, or at least about 5 years after first administration of the composition described herein.

[0144] Corneometer The compositions described herein are believed to contribute to scalp hydration. Scalp hydration is believed to improve hair health and scalp health. Hydration can be assessed, for example, using a corneometer. The electrical properties of the skin and its capacitance are determined primarily by the water content of the stratum corneum. The corneometer is an instrument designed to measure changes in the capacitance of the skin caused by changes in the degree of hydration. It is particularly sensitive to low hydration levels. The corneometer expresses the capacitance of the skin surface in arbitrary units of skin hydration.

[0145] In some embodiments, the methods described herein provide an improvement in scalp moisture retention as measured by a corneometer. In some embodiments, the methods provide an improvement in scalp moisture retention of at least 10% as measured by a corneometer within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months from the first administration of the compositions described herein. In some embodiments, the methods provide an improvement in scalp moisture retention of at least 25% as measured by a corneometer within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months from the first administration of the compositions described herein. In some embodiments, the methods result in at least a 50% improvement in scalp hydration as measured by a corneometer within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the initial administration of a composition described herein.

[0146] sebum meter In some embodiments, the compositions described herein result in a reduction in sebum production on the scalp. Reduction in sebum production on the scalp is believed to improve hair or scalp health, or treat or prevent hair loss. Such reduction in sebum can be measured, for example, using a sebum meter. A sebum meter measures the sebum content of the skin in μg / cm 2 It photometrically measures the increase in transparency of a special translucent plastic strip when it is covered with sebum. The plastic strip is approximately 0.1 mm thick and has an area of ​​64 mm 2 It is housed in the portable head of the instrument. The strip is fitted with a mirror on the back and is pressed by a spring with a fixed pressure of 10 N against the relevant skin. The instrument also contains a timing device that limits the contact time of the strip with the skin to 30 seconds. The transparency of the plastic strip is assessed by a microprocessor and is read directly on the digital instrument as μg of sebum per square centimeter.

[0147] In some embodiments, the method provides a reduction in sebum production on the scalp by at least 10% as measured by a sebum meter within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of the composition described herein. In some embodiments, the method provides a reduction in sebum production on the scalp by at least 25% as measured by a sebum meter within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the first administration of the composition described herein. In some embodiments, the methods result in a reduction in sebum production in the scalp by at least 50% as measured by a sebometer within about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, or about 12 months after the initial administration of a composition described herein.

[0148] pH measurement The pH of the scalp influences the type and number of bacteria that grow in the scalp microbiome. The pH of the skin or scalp can be easily measured, for example, using a skin pH meter. The Skin-pH-Meter PH905 is a fast, easy and economical means to specifically measure the pH of the skin surface or scalp. The measurement of the pH value covers an important property of an aqueous solution, namely its acidity or alkalinity. The surface water and sebum together form a sebum film on the skin, which is also called an acid mantle because its pH value is in the slightly acidic range (about pH 4.0-5.5). In some embodiments, the methods and compositions described herein do not significantly change the pH of the skin. In some embodiments, the methods and compositions described herein maintain the pH of the skin. Since the sebum film is mostly an aqueous solution, the Skin-pH-Meter PH905 allows very accurate, easy and fast pH measurements to be performed directly on the skin surface. The pH value varies depending on the area of ​​the skin. The measurement of the pH value of the skin is performed using a glass electrode. The probe consists of a reference electrode (the outer glass shaft of the probe filled with electrolyte (KCl+AgCl)). Inside there is a second chamber with a measuring electrode in an internal buffer solution. The measuring electrode is in direct contact with the skin via a special flat glass membrane at the tip of the probe, which can fit completely on the skin surface. The reference electrode is in electrical contact with the skin only on the diaphragm in order to equalize the potential. There is no exchange of substances here at all. The modern high-quality electronics of the probe allow very fast and reliable measurements while avoiding occlusion effects. The measurements are carried out in a 3-D chamber with a 100% pH level, which is the standard for measuring the pH of the skin. + It is based on a high quality combined electrode where both the ion sensitive electrode and an additional reference electrode are placed in one glass housing. This is connected to a probe handle which contains the measurement electronics. The probe head is flat and smooth for optimal measurement at any site on the skin surface. One continuous measurement is possible.

[0149] Analysis of scalp microbiome The composition of the scalp microbiota can be determined by gene sequencing. For this, a swab sample can be taken from the center of the scalp (if centrally divided) and processed for sequencing analysis. In this analysis, the composition of the scalp microbiota is sequenced to determine the effect of the test product on viability and colonization.

[0150] In some embodiments, the methods described herein increase the population or proportion of beneficial probiotic bacteria, such as Staphylococcus epidermidis, on the scalp. In some embodiments, the methods described herein decrease the population or proportion of harmful bacteria, such as lipophilic bacteria and yeasts, such as P. acnes and Malassezia species, such as P. acnes and M. furfur, on the scalp. In some embodiments, the compositions and methods described herein prevent the overgrowth of harmful bacteria on the scalp.

[0151] Consumer Perception Survey The efficacy of the compositions and methods described herein can also be determined by questioning subjects regarding consumer perception and effectiveness of the test substances after use. An exemplary consumer perception questionnaire is provided in Example 2.

[0152] manufactured goods Further provided herein is an article of manufacture, e.g., a container, comprising a composition described herein. In some embodiments, the container is a sealed container. In some embodiments, the sealed container is sealed. In some embodiments, the container is plastic. In some embodiments, the plastic is recycled plastic. The article of manufacture can be packaged for retail distribution with instructions advising the consumer how to use the product to treat or prevent hair loss.

[0153] kit In some embodiments, the kit may include any combination of the compositions described herein. For example, in some embodiments, the kit includes a composition useful as a scalp mask and a composition useful as a scalp cleanser. In some embodiments, the kit includes a composition useful as a scalp mask and a composition useful as a scalp essence. In some embodiments, the kit includes a composition useful as a scalp cleanser and a composition useful as a scalp essence. In some embodiments, the kit includes a composition useful as a scalp mask, a composition useful as a scalp cleanser, or a composition useful as a scalp essence. In some embodiments, the kit includes a composition useful as a scalp mask, a composition useful as a scalp cleanser, and a composition useful as a scalp essence. An example of a scalp cleanser is shampoo. EXAMPLES

[0154] The examples described in this section are for illustrative purposes only and are not intended to limit the claimed invention.

[0155] Example 1: Effect of the compositions of Table 3, Table 2, and Table 1 on microbial growth and diversity The composition of Table 3, comprising a co-culture of Staphylococcus epidermidis and Staphylococcus aureus, about 1% to 3% α-glucan oligosaccharides, about 0.1% to 0.3% Bacillus fermentation filtrate or an extract thereof, about 0.1% to 0.3% Opuntia ficus-indica or an extract thereof, and up to about 0.1% peach gum (prunus persica gum); about 0.01% to 0.1% α-glucan oligosaccharides, about 0.01% to 0.1% Kunzea pomifera or an extract thereof, about 0.01% to 0.1% ryeberry (syzygium rumannii), or the composition of Table 2 comprising about 0.1% to 0.3% sarcosine, about 0.01% to 0.1% kunzea pomifera or an extract thereof, about 0.01% to 0.1% ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% pea (pisum sativum) or an extract thereof, and about 0.01% to 0.1% chia (salvia hispanica The cultures were incubated for 15 minutes with a composition according to Table 1 containing C. hispanica) or an extract thereof. Bacterial colonies were counted and normalized to the control.

[0156] The results for the composition in Table 3, the composition in Table 2, and the composition in Table 1 are shown in Figures 1A to 1C, respectively.

[0157] After 15 minutes of incubation with the compositions, the bacterial counts of the opportunistic pathogen S. aureus were reduced to just over 30% of the control bacterial counts for all compositions. The growth of the scalp flora S. epidermidis was reduced compared to the control (100%) for the compositions in Table 1 and Table 2, indicating that these two compositions can regulate the growth of scalp flora without completely preventing its presence, while at the same time maintaining a high ratio of S. epidermidis to S. aureus.

[0158] Co-cultures of Malassezia globosa, Malassezia furfur, Propionibacterium acnes and S. epidermidis were incubated for 15 minutes with the compositions in Table 3, Table 2, or Table 1. Bacterial colonies were counted and normalized to the control. Co-culture allows the microorganisms to interact with each other and mimic scalp conditions. The results for the compositions in Table 3, Table 2, and Table 1 are shown in Figures 2A-2C, respectively.

[0159] The compositions in Table 1 and Table 2 restored balance to microbial diversity by suppressing, without eradicating, the opportunistic pathogen P. acnes associated with acne and the Malassezia yeast associated with dandruff conditions. The compositions were also able to maintain the growth of the scalp flora S. epidermidis, which produces beneficial metabolites. The compositions in Table 3 selectively maintained the growth of the scalp flora S. epidermidis, gently inhibited the growth of the opportunistic pathogen P. acnes, and had no effect on Malassezia species, effectively restoring microbial growth.

[0160] To determine whether the compositions had a bactericidal effect, the compositions in Table 3, Table 2, or Table 1 were applied directly to excess agar plates (>1000 colonies) of individual microorganisms (Malassezia globosa, Malassezia furfur, Propionibacterium acnes, or S. epidermis) and bacterial growth was quantified after application. The results for the compositions in Table 3, Table 2, and Table 1, respectively, are shown in Figures 3A-3C. The effect of each composition on the microorganisms was neither antibacterial nor inhibitory (as not all bacterial growth was stopped), but rather, the product sensitively modulated bacterial growth.

[0161] Example 2: Clinical Trials to Evaluate the Efficacy and Consumer Perception of the Compositions of Table 3, Table 2, and Table 1 This example relates to a test product (composition of Table 2 ("scalp shampoo"); about 0.1% to 0.3% sarcosine, about 0.01% to 0.1% kunzea pomifera or an extract thereof, about 0.01% to 0.1% ryeberry (syzygium luehmannii) or an extract thereof, and about 0.01% to 0.1% tasmannia lanceolata or an extract thereof) containing about 0.01% to 0.1% α-glucan oligosaccharide, about 0.01% to 0.1% kunzea pomifera or an extract thereof, about 0.01% to 0.1% ryeberry (syzygium luehmannii) or an extract thereof, and about 0.01% to 0.1% tasmannia lanceolata or an extract thereof. a composition of Table 1 comprising about 0.01% to 0.1% of Tasmannia lanceolata or extract thereof, about 0.01% to 0.1% of pea (pisum sativum) or extract thereof, and about 0.01% to 0.1% of chia (salvia hispanica) or extract thereof ("exfoliating mask"); or about 1% to 3% of α-glucan oligosaccharides, about 0.1% to 0.3% of Bacillus ferment filtrate or extract thereof, about 0.1% to 0.3% of opuntia ficus-indica or extract thereof, and up to about 0.1% of peach gum (prunus persica This application describes a clinical trial to evaluate the changes made to the scalp following use of the composition of Table 3 ("scalp essence") containing a sebum extract and to ascertain consumer perception of the test product through the use of a questionnaire.

[0162] Test Design Three groups of approximately 40 subjects were enrolled to evaluate scalp changes after use of the test products, obtain scalp images, and take scalp microbiota swabs from randomly selected subjects from each group, and obtain consumer perceptions of the test products using questionnaires. Approximately 40 volunteers tested one test product (scalp shampoo, exfoliating mask, or scalp essence). In total, 33 subjects completed the scalp essence test, 38 subjects completed the exfoliating mask test, and 37 subjects completed the scalp shampoo test.

[0163] Product Instructions For the scalp shampoo evaluation, subjects were given the following instructions: "Apply a small amount to fingertips. Use enough to cover entire scalp. Rub into scalp for 2 minutes and rinse thoroughly with water. Repeat as needed. Use according to your usual schedule. Use at least 3 times per week."

[0164] For the evaluation of the Scalp Essence, subjects were given the following instructions: "Place nozzle directly on scalp. With the top of the bottle, divide hair from front to back into six sections while gently squeezing bottle. Do not rinse. For best results, use daily."

[0165] For evaluation of the exfoliating masks, subjects were given the following instructions: "Separate hair into six sections with the tip of the tube and apply mask directly to scalp. Use enough mask to lightly cover desired areas and rub into scalp with fingertips. Product should cover entire scalp. Leave on for 3-5 minutes. Rinse with water. Use twice a week before shampooing."

[0166] Inclusion and Exclusion Criteria Selection criteria for scalp shampoo group: a) The subjects are women between the ages of 18 and 60 (we will strive to distribute subjects evenly across age groups: 20s, 30s, 40s, and 50+). b) The subjects have either oily scalp (approximately 50% of the study population) or dry scalp (approximately 50% of the study population) as determined by a hairdresser. c) Subjects have self-perceived sensitive skin / scalp (100% of study population). d) The subject suffers from mild to moderate scalp dandruff (which can be from either dry scalp or oily scalp with sebum buildup and dandruff) as confirmed by a hairdresser. e) The subjects have dyed hair (approximately 50% of the study population) or non-dyed hair (approximately 50% of the study population). f) Subjects agree to use their current regular conditioner throughout the entire study period. g) Subject agrees not to introduce any new cosmetics or toiletry products, such as new / different conditioners, during the study period. h) Subject is willing to maintain a regular cleaning schedule. i) Subjects agree not to dye their hair throughout the study period.

[0167] Scalp essence selection criteria: a) The subjects are women aged 18 to 60 years. b) The subject must have a scalp that is moderately dry, flaky, and inflamed (red) as determined by a hairdresser. c) Subjects are willing to continue using their usual shampoo and conditioner. d) Subjects agree not to dye their hair and to maintain the same hairstyle throughout the study period. e) The subject is aware that his / her hair is thinning. f) Subjects were aware that their scalp was sensitive (100%). g) Subjects must have colored hair (at least 50% of the population). h) Subject agrees not to bring any new cosmetics or toiletry products, such as shampoos or conditioners, to the study site during the study period.

[0168] Exfoliating mask selection criteria: a) The subjects are women aged 18 to 60 years. b) Subjects must have moderate to excessive sebum on the scalp as determined by a hairdresser. c) The subject perceives that if the hair is not washed for 24 hours, a moderate amount of pale yellow sebum accumulates. d), e) Subjects must have colored or uncolored hair (minimum 50% of the population has colored hair and maximum 50% of the population has uncolored hair). f) Subjects were aware that they had a sensitive scalp (100%). g) Subject agrees to bring shampoo and conditioner to the baseline study visit. h) Subjects agree to wash their hair in the ECRL at the baseline visit. i) The subject agrees not to bring any new cosmetics or toiletry products, such as shampoos or conditioners, into the testing site during the study.

[0169] Exclusion Criteria (for all groups) a) The subject is pregnant, nursing, planning to become pregnant, or not using adequate contraception. b) Subject is currently using, or has used within the past week, systemic or topical corticosteroids, nonsteroidal anti-inflammatory drugs, antihistamines, sympathomimetics, and / or vasoconstrictors. c) The subject has a known allergy to skin treatment products or cosmetics, toiletry products, and / or topical medications. d) The subject has a history of acute or chronic dermatological, medical, and / or physical illness that may interfere with the application of the test substance and / or affect the outcome of the study. e) Subjects have inflammation, tattoos, sunburn, rashes, scratches, burn marks, scars, etc. at the test site that may interfere with the evaluation of the test results. f) The subject is currently taking medications that may interfere with the study. g) Subject has a history of skin cancer or is currently being treated for any active cancer. h) The subject has diabetes.

[0170] Exam evaluation Corneometer At each designated time point, three corneometer measurements were taken from the top center of the scalp when the hair was parted in the middle.

[0171] sebum meter Using a Sebumeter SM810PC, one Sebumeter measurement was obtained from the top center of the scalp when the hair was parted in the middle at each designated time point.

[0172] pH measurement Using a Skin-pH-Meter PH905, one pH meter reading was obtained from the top center of the scalp when the hair was parted in the middle at each specified time point.

[0173] FotoFinder Medicam Images were taken in Trichogram mode and data were generated using the Trichoscale program. The following parameters were collected for analysis: "Overview" images of the scalp were taken with a Medicam 1000 and close-up images of the scalp (with hair sectioned in the middle) were taken with a Medicam 1000+D-Scope IV20x.

[0174] Expert clinical grading Clinical grading from scalp images (when cut at the center) was performed by a hairstylist for the following flakiness and redness parameters using the scoring scale shown in FIG.

[0175] Hair count after combing Each subject's hair was divided into four quadrants: front left, front right, back left, and back right. If the hair was not long enough to separate, it was combed to form the four quadrants without being separated. Subjects combed their hair over a piece of white paper, combing twice through each quadrant for a total of eight times. The hair that fell onto the white surface was counted.

[0176] Cotton swab kit At each designated time point, one swab sample was obtained from the central scalp (centrally divided) of 15 subjects (randomized), stored at -80°C, and processed for sequencing analysis. The composition of the scalp microbiota was sequenced to determine the effect of the test products on viability and colonization.

[0177] Consumer Perception Survey The properties of the test materials and the evaluation of the efficacy of the test materials were determined by questioning the treated subjects regarding consumer perception and efficacy of the test materials after use. The consumer perception questionnaire could be answered with "strongly agree", "agree", "disagree", "strongly disagree", or "not applicable". The questionnaires regarding consumer perception of scalp shampoo, scalp essence, and exfoliating mask are shown in Table 4, Table 5, and Table 6, respectively.

[0178] Table 4: Scalp shampoo evaluation questionnaire [Table 5]

[0179] Table 5: Scalp essence evaluation questionnaire [Table 6]

[0180] Table 6: Exfoliating mask evaluation questionnaire [Table 7]

[0181] Test Method The following protocol was used for the three groups.

[0182] Scalp shampoos: Screening: Subjects were instructed to report to the study site that they had not washed their hair for the past 24-36 hours. Informed consent was obtained. Inclusion and exclusion criteria were reviewed. Subjects were asked to continue using their own shampoo and conditioner and to discontinue use of other hair care products. Subjects were instructed to bring their own shampoo and conditioner at baseline.

[0183] Baseline: Subjects returned to the testing facility without having washed their hair for the past 24-36 hours. Subjects were allowed to acclimate to the environmental conditions of the laboratory for approximately 15 minutes (± 5 minutes). The following test assessments were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, and Corneometer measurements. An ECRL hair stylist washed the subject's hair with a scalp shampoo test product and applied the subject's conditioner in the laboratory. The hair stylist then blow-dried the subject's hair.

[0184] Immediately after application / blow dry (± 20 min): After the test material was applied and blow dried for 20 minutes, the following test evaluations were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, Corneometer measurements, and a consumer perception questionnaire. Test materials, instructions, and a diary were distributed.

[0185] Day 7: Subjects returned to the testing facility without having washed their hair for the past 24-36 hours. Subjects were allowed to acclimate to the environmental conditions of the laboratory for approximately 15 minutes (± 5 minutes). The following test assessments were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, Corneometer measurements, and a consumer perception questionnaire.

[0186] Day 14 (final visit): Subjects returned to the study facility without having washed their hair for the past 24-36 hours. Used and unused materials were collected. Diaries were examined for study compliance and collected. Subjects were acclimated to the environmental conditions of the laboratory for approximately 15 minutes (± 5 minutes). The following study assessments were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, Corneometer measurements, and a consumer perception questionnaire.

[0187] Scalp essence group: Screening: Subjects were instructed to report to the study site. Informed consent was obtained. Inclusion and exclusion criteria were verified. Subjects were asked to continue using their own shampoo and conditioner and to discontinue use of other hair care products.

[0188] Baseline: Subjects returned to the study facility. Subjects were allowed to acclimate to the laboratory conditions for approximately 15 minutes (± 5 minutes). The following study assessments were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, Corneometer measurements, and scalp swabs for 15 randomized subjects. Hair retention assessment was performed by a trained clinical grader. Study materials, diaries, and instructions for use were distributed.

[0189] Day 7: Subjects returned to the testing facility. Subjects were allowed to acclimate to the laboratory conditions for approximately 15 minutes (± 5 minutes). The following test evaluations were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, Corneometer measurements, and a consumer perception questionnaire. Hair retention evaluation was performed by a trained clinical grader.

[0190] Day 14: Subjects returned to the testing facility. Subjects were allowed to acclimate to the laboratory conditions for approximately 15 minutes (± 5 minutes). The following test evaluations were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, Corneometer measurements, and Consumer Perception Questionnaire. Hair retention evaluation was performed by a trained clinical grader.

[0191] Day 28 (final visit): Subjects returned to the study facility. Used and unused materials were collected. Diaries were checked for study compliance and collected. Subjects were acclimated to the laboratory conditions for approximately 15 minutes (± 5 minutes). The following study assessments were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, Corneometer measurements, scalp swabs of 15 randomized subjects, and a consumer perception questionnaire. Hair retention assessment was performed by a trained clinical grader.

[0192] Exfoliating masks: Screening: Subjects were instructed to report to the study site that they had not washed their hair for the past 24-36 hours. Informed consent was obtained. Inclusion and exclusion criteria were verified. Subjects were asked to continue using their own shampoo and conditioner and to discontinue use of other hair care products. Subjects were instructed to bring their own shampoo and conditioner at baseline.

[0193] Baseline: Subjects returned to the study facility without having washed their hair for the past 24-36 hours. Subjects were allowed to acclimate to the environmental conditions of the laboratory for approximately 15 minutes (± 5 minutes). The following study assessments were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, and scalp swabs for 15 randomized subjects. An ECRL hair stylist washed the subjects' hair with a scalp shampoo study product and applied the subjects' conditioner in the laboratory. The hair stylist then blow-dried the subjects' hair. An expert clinician score for sebum build-up was made from the photographs. Under the supervision of an ECRL technician, subjects shampooed and conditioned their hair (with their own hair products) in the laboratory. An exfoliating mask was then applied and their hair was blow-dried.

[0194] Immediately after application / blow dry (± 20 min): After applying the test material and blow drying for 20 minutes, the following test evaluations were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, and a consumer perception questionnaire. Expert clinical evaluation of sebum buildup was performed from the photographs. Test material, instructions for use, and a diary were distributed.

[0195] Day 7: Subjects returned to the testing facility without having washed their hair for the past 24-36 hours. Subjects were allowed to acclimate to the laboratory conditions for approximately 15 minutes (± 5 minutes). The following test evaluations were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, and a consumer perception questionnaire. An expert clinician score of sebum accumulation was performed from the photographs.

[0196] Day 14: Subjects returned to the testing facility without having washed their hair for the past 24-36 hours. Subjects were allowed to acclimate to the environmental conditions of the laboratory for approximately 15 minutes (± 5 minutes). The following test evaluations were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, and a consumer perception questionnaire. Expert clinical scoring of sebum accumulation was performed from the photographs.

[0197] Day 28 (final visit): Subjects returned to the study facility without having washed their hair for the previous 24-36 hours. Used and unused materials were collected. Diaries were checked for study compliance and collected. Subjects were acclimated to the environmental conditions of the laboratory for approximately 15 minutes (± 5 minutes). The following study evaluations were performed: FotoFinder Medicam 1000 images, Sebumometer measurements, pH measurements, scalp swabs of 15 randomized subjects, and a consumer perception questionnaire. Expert clinical scoring of sebum accumulation was performed from the photographs.

[0198] Data Table Creation Analysis of variance followed by Dunnett's test was used to determine the differences between baseline and each post-treatment interval for the following parameters: FotoFinder Medicam 1000 images, Corneometer measurements, Sebometer measurements, pH meter measurements, clinical scores from scalp images, and hair retention assessment. Changes from baseline and % of subjects improved were calculated for the above parameters at each post-treatment interval.

[0199] Statistically significant differences exist at a two-sided p-value of 0.05. Survey responses where comparison of response categories was informative were analyzed by Z-test. Z-tests were used to ascertain statistically significant differences in the proportion of subjects responding positively or negatively to each question where a range of responses was presented. Where applicable, the proportion of subjects who selected a middle (neutral) response was divided equally and added to the proportion of responses of the top and bottom options. The divided proportions were compared by calculation of Z-scores to determine statistical significance. At the 95% confidence level, statistical significance exists if the absolute value of the Z-score is 1.96 or greater. Swabs were stored at -80°C and sent to a third-party laboratory for microbiota analysis.

[0200] Adverse events An adverse event is any undesirable medical occurrence, including death, experienced by a subject, whether or not considered related to the study. An event may consist of a disease, an exacerbation of an existing disease or condition, an intermittent occurrence of a disease or condition, a series of related symptoms or signs, or a single symptom or sign. A serious adverse event refers to any event that places a subject at imminent risk of death, or a persistent or significant inability or significant interruption in the ability to perform normal life functions.

[0201] All adverse events were recorded promptly. All adverse events, whether serious or not, and whether or not related to the test substance, were summarized and reported in the final report. All adverse events were followed until resolved, stabilized, unknown on subject follow-up, or the event was otherwise explained. All reports of skin irritation or sensory experiences were documented as experimental data in the clinical trial report and were not considered adverse events unless confirmed in the laboratory by medical staff.

[0202] result Scalp shampoo group After using the scalp shampoo for 14 days, the subjects' sebum content was significantly reduced (18.9%, p<0.001) (Figure 5A). The sebum levels of the dry group (baseline sebum <50) were reduced by 16% over the 14 days of using the scalp shampoo. The oily group (baseline sebum >50) were reduced by 44% over the 14 days of using the scalp shampoo (Figure 5B).

[0203] The pH of the subjects' scalp did not change significantly (p>0.05), indicating that the scalp shampoo maintains pH over time (Figure 6A). This was observed in both the oily and dry groups (Figure 6B). Figure 17 shows the change in pH over 14 days of scalp shampoo use in the dry and oily groups, which were determined by expert assessment. There was no change in scalp pH with the use of the scalp shampoo (overall % change <2%).

[0204] Scalp moisture, as measured by a corneometer, improved significantly from baseline by 7.5% (p<0.01) after 7 days and 7.6% (p<0.01) after 14 days (Figure 7A). Moisture in the dry scalp group improved by 13% during the 14 days of scalp shampoo use, and in the oily scalp group, it improved by 32% during the 14 days of scalp shampoo use (Figure 7B). Figure 18 shows the scalp moisture measured by a corneometer after scalp shampoo use (14 days) for the dry and oily scalp groups, as determined by expert assessors. Moisture in the dry scalp group improved by 25% during the 14 days of scalp shampoo use. In the oily scalp group, it improved by 19% during the 14 days of scalp shampoo use.

[0205] Dandruff in the subjects' scalp, as measured by expert dermatologist ratings, improved significantly from baseline by 4.6% (p<0.0001) after 7 days and 4.7% (p<0.0001) after 14 days with scalp shampoo (Figure 8A). Expert dandruff scores improved by 75% in the dry group and 82% in the oily group after 14 days of scalp shampoo use (Figure 8B). Figure 16 shows the change in sebum content over time in the dry and oily groups, where dry and oily conditions were determined by expert raters. Dry group sebum levels decreased by 32% over 14 days of scalp shampoo use. Oily group (baseline sebum >50) decreased by 37% over 14 days of scalp shampoo use. Figure 19 shows the scalp dandruff measured by an expert evaluator (dermatologist) after 14 days of use of the scalp shampoos for dry and oily groups, where the dry and oily groups were judged by an expert evaluator. After 14 days of use of the scalp shampoos, the expert's dandruff assessment showed an improvement of 74% in the dry group and 83% in the oily group.

[0206] Exfoliating Mask Subjects' scalp sebum measurements, as measured by a sebometer, were reduced by 94% from baseline immediately after use of the exfoliating mask (p<0.0001), by 30% after 7 days (p=0.02), by 30% after 14 days (p=0.02), and by 38% after 28 days (p=0.002) (Figure 9). 100% of subjects experienced immediate improvement, 55.3% of subjects experienced improvement after 7 days, 55.3% of subjects experienced improvement after 14 days, and 65.8% of subjects experienced improvement after 28 days. Sebum accumulation scores, as measured by expert clinical grading, decreased over the first 28 days of use, decreasing by 16.4% after 7 days (p<0.0001), 21.3% after 14 days (p<0.0001), and 29.5% after 28 days (p<0.0001). 97.4% of subjects improved immediately, 76.3% of subjects improved after 7 days, 78.9% of subjects improved after 14 days, and 92.1% of subjects improved after 28 days. Table 7 shows the survey results, consumer self-perception results regarding exfoliating masks. Only statistically significant survey responses, as assessed by Z-test scores, are reported.

[0207] Table 7: Survey results, consumer self-awareness results regarding exfoliating masks [Table 8]

[0208] Scalp pH did not change significantly over one month of exfoliating mask use at days 7 (p>0.05), 14 (p>0.05), and 28 (p>0.05) (Figure 10).

[0209] Scalp Essence After using the scalp essence for 28 days, no significant improvement in moisture retention was observed, but a trend toward increased moisture retention was observed. After 28 days, 60.6% of subjects experienced improvement in moisture retention (Figure 11).

[0210] After 14 days of use, sebum content measured by the Sebum Meter was reduced by 45.6% from baseline (p=0.02), but there was no significant improvement compared to baseline at day 28, indicating that sebum levels had regulated back to baseline conditions (Figure 12). After 7 days, 72.7% of subjects showed a reduction in sebum content, after 14 days, 72.7% of subjects showed a reduction in sebum content, and after 28 days, 66.7% of subjects showed a reduction in sebum content.

[0211] After using the scalp essence for 28 days, no significant change in pH was observed (p>0.05) (Figure 13), indicating that the pH of the scalp was maintained.

[0212] Hair loss, as measured by the total number of hairs in the four quadrants collected from the brush, improved significantly after 28 days of Scalp Essence use (31% reduction compared to baseline, p=0.04) (Figure 14A). After 28 days, hair counts (sum of all four quadrants) improved in 60.6% of subjects. The number of hairs with a found root improved after 7 days (71%, p<0.001), 14 days (79%, p<0.001), and 28 days (47%, p<0.05) (Figure 14B). The number of hairs without roots (broken hairs) improved after 14 days of Essence use (45%, p<0.05), and after 28 days of Scalp Essence use, the total number of counted hairs also improved (31%, p<0.05) (Figure 14B). 72.7% of subjects improved after 7 days, 69.7% of subjects improved after 14 days, and 63.6% improved after 28 days.

[0213] Dandruff as determined by expert clinical grading improved after 28 days of scalp essence use (33%, p=0.0016) (Figure 15). 66.7% of subjects had improved dandruff after 18 days (expert clinical grading). Similarly, redness as determined by expert clinical grading improved after 28 days of scalp essence use (26.5%, p=0.0004) (Figure 15). 60.6% of subjects had improved redness after 28 days (expert clinical grading).

[0214] Table 8 shows the survey results and consumer self-perception results regarding Scalp Essence. Only statistically significant survey responses, as assessed by Z-test scores, are reported.

[0215] Table 8: Survey results, consumer self-awareness results regarding scalp essence [Table 9]

Claims

1. about 0.1% to 0.3% by weight of sarcosine, about 0.01% to 0.1% by weight of kunzea pomifera or an extract thereof, about 0.01% to 0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% by weight of tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% by weight of pea (pisum sativum) or an extract thereof, about 0.01% to 0.1% by weight of chia (salvia hispanica hispanica) or extract thereof, about 0.1% to 0.3% by weight of vaccinium myrtillus or extract thereof, about 0.1% to 0.3% by weight of saccharum officinarum (sugar cane) or extract thereof, about 0.01% to 0.1% by weight of citrus aurantium dulcis (orange) or extract thereof, about 0.01% to 0.1% by weight of citrus limon (lemon) or extract thereof, about 0.01% to 0.1% by weight of acer saccharum (acer saccharum ... citrus aurantium dulcis (orange) or extract thereof, about 0.01% to 0.1% by weight of citrus aurantium dulcis (o saccharum (sugar maple) or an extract thereof and about 1% to 3% by weight of jojoba esters, Simmondsia chinensis or an extract thereof.

2. About 30% by weight water, about 3% to 10% by weight glycerin, about 1% to 3% by weight cetearyl alcohol, about 1% to 3% by weight brassicamidopropyl dimethylamine, about 1% to 3% by weight fragrance, about 0.3% to 0.99% by weight polyquatemium-37, about 0.3% to 0.99% by weight lactic acid, about 0.3% to 0.99% by weight sodium benzoate, about 0.1% to 0.3% by weight piroctone olamine, about 0.1% to 0.3% by weight potassium sorbate, about 0.01% to 0.1% by weight tetrasodium glutamate diacetate, about 0.01% to 0.1% by weight montmorillonite, about 0.01% to 0.1% by weight CI 77289 (chromium hydroxide green), about 0.01% to 0.1% by weight isopropyl alcohol, about 0.01% to 0.1% by weight sodium hydroxide, about 0.01% to 0.1% by weight tetrasodium EDTA, and / or about 0.01% to 0.1% by weight Aspergillus ferment.

3. The composition may comprise any of kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, tasmannia lanceolata extract, pea (pisum sativum) extract, chia (salvia hispanica) extract, sugarcane (saccharum officinarum) extract, citrus aurantium dulcis extract, citrus limon (citrus limon) extract, citrus oleracea (citrus limon) extract, citrus serrata (citrus serrata ... limon extract, and / or acer saccharum extract, including the above kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, tasmannia lanceolata extract, pea (pisum sativum) extract, chia (salvia hispanica) extract, sugarcane (saccharum officinarum) extract, citrus aurantium dulcis extract, 3. The composition of claim 1 or 2, wherein the extract of Citrus dulcis, Citrus limon, and Acer saccharum is a fruit extract, a leaf extract, a root extract, or a combination thereof.

4. The composition may be selected from the group consisting of kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, tasmannia lanceolata extract, pea (pisum sativum) extract, chia (salvia hispanica) extract, sugarcane (saccharum officinarum) extract, citrus aurantium dulcis extract, citrus limon (citrus limon) extract, and the like. limon extract, or acer saccharum extract, including the above kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, tasmannia lanceolata extract, pea (pisum sativum) extract, chia (salvia hispanica) extract, sugarcane (saccharum officinarum) extract, citrus aurantium dulcis extract, 3. The composition of claim 1 or 2, wherein the extract of Citrus dulcis, the extract of Citrus limon, and the extract of Acer saccharum are whole plant extracts.

5. The composition of any one of claims 1 to 4, further comprising a cosmetically acceptable carrier or excipient.

6. A composition according to any one of claims 1 to 5, wherein the composition is formulated for use as a scalp mask.

7. 10. A method of improving hair health or scalp health, or treating or preventing hair loss, in a subject in need thereof, comprising applying to the scalp of the subject an effective amount of a composition according to any one of claims 1 to 6.

8. 8. The method of claim 7, further comprising allowing the composition to remain on the scalp for at least about 5 to about 10 minutes and rinsing the composition from the scalp.

9. 9. The method of claim 7 or 8, comprising applying the composition twice weekly.

10. 10. The method of any one of claims 7-9, wherein: (a) the administration is the first administration of the composition; (b) the method results in an improvement in scalp hydration of at least 10% within about 1 month, about 2 months, or about 3 months of the first administration of the composition; and (c) the improvement is measured using a corneometer.

11. 11. The method of any one of claims 7 to 10, wherein: (a) the administering is a first administration of the composition; (b) the method results in at least a 10% reduction in sebum production in the scalp within about 1 month, about 2 months, or about 3 months of the first administration of the composition; and (c) the reduction is measured using a sebometer.

12. 1. A composition comprising about 0.01% to 0.1% by weight of α-glucan oligosaccharides, about 0.01% to 0.1% by weight of kunzea pomifera or an extract thereof, about 0.01% to 0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% by weight of tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% by weight of hydrolyzed quinoa, and about 0.01% to 0.1% by weight of Aspergillus fermentation product.

13. About 30% by weight water, about 3% to 10% by weight sodium C14-16 olefin sulfonate, about 3% to 10% by weight cocamidopropyl betaine, about 3% to 10% by weight sodium lauroyl sarcosinate, about 1% to 3% by weight propanediol, about 1% to 3% by weight sodium chloride, about 0.3% to 0.99% by weight fragrance, about 0.3% to 0.99% by weight cocamide MIPA, about 0.3% to 0.99% by weight glycol distearate, about 0.3% to 0.99% by weight citric acid, about 0.3% to 0.99% by weight hydroxypropyl methylcellulose, about 0.3% to 0.99% by weight guar hydroxypropyltrimonium chloride, about 0.3% to 0.99% by weight glycerin, about 0.3% to 0.99% by weight 13. The composition of claim 12, further comprising about 0.01% to 0.1% by weight of tetrasodium glutamate diacetate, about 0.01% to 0.1% by weight of diisopropyl adipate, about 0.01% to 0.1% by weight of sodium hydroxide, about 0.01% to 0.1% by weight of triethyl citrate, about 0.01% to 0.1% by weight of caprylyl glyceryl ether, and / or about 0.01% to 0.1% by weight of benzyl alcohol.

14. 14. The composition of claim 12 or 13, wherein the composition comprises a kunzea pomifera extract, a ryeberry (syzygium luehmannii) extract, and / or a tasmannia lanceolata extract, wherein the kunzea pomifera extract, the ryeberry (syzygium luehmannii) extract, and / or the tasmannia lanceolata extract are a fruit extract, a leaf extract, a root extract, or a combination thereof.

15. 14. The composition of claim 12 or 13, wherein the composition comprises an extract of kunzea pomifera, an extract of ryeberry (syzygium luehmannii), and / or an extract of tasmannia lanceolata, wherein the extract of kunzea pomifera, an extract of ryeberry (syzygium luehmannii), and / or an extract of tasmannia lanceolata are whole plant extracts.

16. The composition of any one of claims 12 to 15, further comprising a cosmetically acceptable carrier or excipient.

17. The composition according to any one of claims 12 to 16, wherein the composition is useful as a shampoo.

18. 18. A method of improving hair health or scalp health, or treating or preventing hair loss, in a subject in need thereof, comprising applying to the scalp of the subject an effective amount of a composition according to any one of claims 12 to 17.

19. 20. The method of claim 18, further comprising rinsing the composition from the subject's scalp.

20. 20. The method of claim 18 or 19, comprising applying the composition once a day.

21. 21. The method of any one of claims 18 to 20, wherein: (a) the administration is an initial administration of the composition; (b) the method results in an improvement in scalp hydration of at least 10% within about 1 month, about 2 months, or about 3 months of the initial administration of the composition; and (c) the improvement is measured using a corneometer.

22. 22. The method of any one of claims 18 to 21, wherein: (a) the administering is the first administration of the composition; (b) the method results in at least a 10% reduction in sebum production in the scalp within about 1 month, about 2 months, or about 3 months of the first administration of the composition; and (c) the reduction is measured using a sebometer.

23. A composition comprising about 1% to 3% by weight of α-glucan oligosaccharides, about 0.1% to 0.3% by weight of Bacillus fermentation filtrate or an extract thereof, about 0.1% to 0.3% by weight of Opuntia ficus-indica or an extract thereof, about 0.01% to 0.1% by weight of peach gum (prunus persica gum), and about 1% to 3% by weight of smithsonite or an extract thereof.

24. About 30% by weight water, about 1% to 3% by weight glycerin, about 1% to 3% by weight propanediol, about 0.3% to 0.99% by weight caprylyl / capryl glucoside, about 0.3% to 0.99% by weight guar hydroxypropyltrimonium chloride, about 0.3% to 0.99% by weight sodium benzoate, about 0.1% to 0.3% by weight potassium sorbate, about 0.1% to 0.3% by weight hydrolyzed quinoa, about 0.1% to 0.3% by weight fragrance, about 0.1% to 0.3% by weight lactic acid, about 0.1% to 0.3% by weight Zingiber officinale 24. The composition of claim 23, further comprising: Ginger (Zingiber officinale) or an extract thereof; about 0.01% to 0.1% by weight tetrasodium glutamic acid diacetate; about 0.01% to 0.1% by weight diisopropyl adipate; about 0.01% to 0.1% by weight triethyl citrate; about 0.01% to 0.1% by weight sodium hydroxide; or about 0.01% to 0.1% by weight benzyl alcohol.

25. 25. The composition of claim 23 or 24, wherein the composition comprises an extract of opuntia ficus-indica and / or an extract of zingiber officinale, the extract of opuntia ficus-indica and / or the extract of zingiber officinale being a fruit extract, a leaf extract, a root extract, or a combination thereof.

26. 26. The composition of any one of claims 23 to 25, wherein the composition comprises an extract of Opuntia ficus-indica and / or an extract of Zingiber officinale, the extract of Opuntia ficus-indica and / or the extract of Zingiber officinale being a whole plant extract.

27. A composition according to any one of claims 23 to 26, further comprising a cosmetically acceptable carrier or excipient.

28. The composition according to any one of claims 23 to 27, wherein the composition is useful as a scalp essence.

29. 29. A method of improving hair health or scalp health, or treating or preventing hair loss, in a subject in need thereof, comprising applying to the scalp of the subject an effective amount of a composition according to any one of claims 23 to 28.

30. 30. The method of claim 29, comprising applying the composition once a day.

31. 31. The method of claim 29 or 30, comprising allowing the composition to remain on the scalp for at least 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, or 48 hours.

32. The method of any one of claims 29 to 31, further comprising rinsing the composition from the scalp.

33. 33. The method of any one of claims 29-32, wherein: (a) the administration is an initial administration of the composition; (b) the method results in an improvement in scalp hydration of at least 10% within about 1 month, about 2 months, or about 3 months of the initial administration of the composition; and (c) the improvement is measured using a corneometer.

34. 33. The method of any one of claims 29-32, wherein: (a) the administering is the first administration of the composition; (b) the method results in at least a 10% reduction in sebum production in the scalp within about 1 month, about 2 months, or about 3 months of the first administration of the composition; and (c) the reduction is measured using a sebometer.

35. 1. A method of improving hair health or scalp health or treating or preventing hair loss in a subject in need thereof, comprising administering to the scalp of the subject about 0.1% to 0.3% by weight of sarcosine, about 0.01% to 0.1% by weight of kunzea pomifera or an extract thereof, about 0.01% to 0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% by weight of tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% by weight of pea (pisum sativum), sativum) or extract thereof, about 0.01% to 0.1% by weight of chia (salvia hispanica) or extract thereof, about 0.1% to 0.3% by weight of vaccinium myrtillus or extract thereof, about 0.1% to 0.3% by weight of saccharum officinarum (sugar cane) or extract thereof, about 0.01% to 0.1% by weight of citrus aurantium dulcis (orange) or extract thereof, about 0.01% to 0.1% by weight of citrus limon (citrus limon ... citrus limon (citrus limon) or extract thereof, about 0.01% to 0.1% by weight of citrus limon (citrus limon) or extract thereof, about 0.01% to 0.1% by weight of citrus limon (citrus limon) or extract thereof, about 0.01% to 0.1% by weight of citrus limon (citrus limon) or extract thereof, about 0.01% to 0.1% by weight of citrus limon (citrus limon) or extract thereof, about 0. limon (lemon) or extract thereof, about 0.01% to 0.1% by weight of acer saccharum (sugar maple) or extract thereof, and about 1% to 3% by weight of jojoba esters, Simmondsia chinensis (Simmondsia chinensis) or extract thereof.

36. A subject's scalp is applied with about 30% by weight water, about 3% to 10% by weight glycerin, about 1% to 3% by weight cetearyl alcohol, about 1% to 3% by weight brassicamidopropyl dimethylamine, about 1% to 3% by weight fragrance, about 0.3% to 0.99% by weight polyquatemium-37, about 0.3% to 0.99% by weight lactic acid, about 0.3% to 0.99% by weight sodium benzoate, about 0.1% to 0.3% by weight piroctone olamine, about 0.1% to 0.3% by weight potassium sorbate, about 0.01% to 0.1% by weight tetrasodium glutamate diacetate, about 0.01% to 0.1% by weight montmorillonite, about 0.01% to 0.1% by weight CI 36. The method of claim 35, further comprising applying 77289 (chromium hydroxide green), about 0.01% to 0.1% by weight isopropyl alcohol, about 0.01% to 0.1% by weight sodium hydroxide, about 0.01% to 0.1% by weight tetrasodium EDTA, and / or about 0.01% to 0.1% by weight Aspergillus ferment.

37. The subjects' scalps were treated with kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, tasmannia lanceolata extract, pea (pisum sativum) extract, chia (salvia hispanica) extract, sugarcane (saccharum officinarum) extract, citrus aurantium dulcis extract, citrus limon (citrus limon) extract, and lavender (lavender) extract. limon extract, and / or acer saccharum extract, such as kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, tasmannia lanceolata extract, pea (pisum sativum) extract, chia (salvia hispanica) extract, sugarcane (saccharum officinarum) extract, citrus aurantium dulcis extract, citrus serrata ...

37. The method of claim 35 or 36, wherein the extract of Citrus dulcis, Citrus limon, and Acer saccharum is a fruit extract, a leaf extract, a root extract, or a combination thereof.

38. The subjects' scalps were treated with kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, tasmannia lanceolata extract, pea (pisum sativum) extract, chia (salvia hispanica) extract, sugarcane (saccharum officinarum) extract, citrus aurantium dulcis extract, citrus limon (citrus limon) extract, and lavender (lavender) extract. limon extract, or acer saccharum extract, such as kunzea pomifera extract, ryeberry (syzygium luehmannii) extract, tasmannia lanceolata extract, pea (pisum sativum) extract, chia (salvia hispanica) extract, sugarcane (saccharum officinarum) extract, citrus aurantium dulcis extract, or the like.

37. The method of claim 35 or 36, wherein the extract of N. dulcis, the extract of citrus limon, and the extract of acer saccharum are whole plant extracts.

39. 39. The method of any one of claims 35 to 38, further comprising rinsing the subject's scalp at least about 5 to about 10 minutes after said application.

40. The method according to any one of claims 35 to 39, wherein said application is a twice weekly application.

41. 41. The method of any one of claims 35-40, wherein: (a) the application is a first application; (b) the method results in an improvement in scalp hydration of at least 10% within about 1 month, about 2 months, or about 3 months of the first application; and (c) the improvement is measured using a corneometer.

42. 41. The method of any one of claims 35-40, wherein: (a) the application is a first application; (b) the method results in a reduction in sebum production in the scalp by at least 10% within about 1 month, about 2 months, or about 3 months of the first application; and (c) the reduction is measured using a sebometer.

43. 1. A method of improving hair health or scalp health or treating or preventing hair loss in a subject in need thereof, comprising applying to the scalp of the subject about 0.01% to 0.1% by weight of α-glucan oligosaccharides, about 0.01% to 0.1% by weight of kunzea pomifera or an extract thereof, about 0.01% to 0.1% by weight of ryeberry (syzygium luehmannii) or an extract thereof, about 0.01% to 0.1% by weight of tasmannia lanceolata or an extract thereof, about 0.01% to 0.1% by weight of hydrolyzed quinoa, and about 0.01% to 0.1% by weight of Aspergillus fermentate.

44. A subject's scalp is applied with about 30% by weight water, about 3% to 10% by weight sodium C14-16 olefin sulfonate, about 3% to 10% by weight cocamidopropyl betaine, about 3% to 10% by weight sodium lauroyl sarcosinate, about 1% to 3% by weight propanediol, about 1% to 3% by weight sodium chloride, about 0.3% to 0.99% by weight fragrance, about 0.3% to 0.99% by weight cocamide MIPA, about 0.3% to 0.99% by weight glycol distearate, about 0.3% to 0.99% by weight citric acid, about 0.3% to 0.99% by weight hydroxypropyl methylcellulose, about 0.3% to 0.99% by weight guar hydroxypropyltrimonium chloride, about 0.3% to 0.99% by weight glycerin ... about 0.3% to 0.99% by weight of polyquatemium-73, about 0.1% to 0.3% by weight of caprylyl / capryl glucoside, about 0.1% to 0.3% by weight of potassium sorbate, about 0.1% to 0.3% by weight of glycolipids, about 0.01% to 0.1% by weight of tetrasodium glutamate diacetate 44. The method of claim 43, further comprising applying about 0.01% to 0.1% by weight of diisopropyl adipate, about 0.01% to 0.1% by weight of sodium hydroxide, about 0.01% to 0.1% by weight of triethyl citrate, about 0.01% to 0.1% by weight of caprylyl glyceryl ether, and / or about 0.01% to 0.1% by weight of benzyl alcohol.

45. 45. The method of claim 43 or 44, comprising applying to the scalp of a subject an extract of kunzea pomifera, an extract of ryeberry (syzygium luehmannii), and / or an extract of tasmannia lanceolata, wherein the extract of kunzea pomifera, an extract of ryeberry (syzygium luehmannii), and / or an extract of tasmannia lanceolata is a fruit extract, a leaf extract, a root extract, or a combination thereof.

46. 45. The method of claim 43 or 44, comprising applying a kunzea pomifera extract, a ryeberry (syzygium luehmannii) extract, and / or a tasmannia lanceolata extract to the scalp of a subject, wherein the kunzea pomifera extract, the ryeberry (syzygium luehmannii) extract, and / or the tasmannia lanceolata extract are whole plant extracts.

47. 47. The method of any one of claims 43 to 46, further comprising rinsing the subject's scalp.

48. The method according to any one of claims 43 to 47, wherein said application is a once-daily application.

49. 49. The method of any one of claims 43-48, wherein: (a) the administration is an initial administration of the composition; (b) the method results in an improvement in scalp hydration by at least 10% within about 1 month, about 2 months, or about 3 months of the initial administration of the composition; and (c) the improvement is measured using a corneometer.

50. 50. The method of any one of claims 43-49, wherein: (a) the administering is the first administration of the composition; (b) the method results in at least a 10% reduction in sebum production in the scalp within about 1 month, about 2 months, or about 3 months of the first administration of the composition; and (c) the reduction is measured using a sebometer.

51. 1. A method of improving hair health or scalp health or treating or preventing hair loss in a subject in need thereof, comprising applying to the scalp of the subject about 1% to 3% by weight of α-glucan oligosaccharides, about 0.1% to 0.3% by weight of Bacillus ferment filtrate or an extract thereof, about 0.1% to 0.3% by weight of Opuntia ficus-indica or an extract thereof, about 0.01% to 0.1% by weight of prunus persica gum, and about 1% to 3% by weight of smithsonite or an extract thereof.

52. A subject's scalp was administered about 30% by weight of water, about 1% to 3% by weight of glycerin, about 1% to 3% by weight of propanediol, about 0.3% to 0.99% by weight of caprylyl / capryl glucoside, about 0.3% to 0.99% by weight of guar hydroxypropyltrimonium chloride, about 0.3% to 0.99% by weight of sodium benzoate, about 0.1% to 0.3% by weight of potassium sorbate, about 0.1% to 0.3% by weight of hydrolyzed quinoa, about 0.1% to 0.3% by weight of fragrance, about 0.1% to 0.3% by weight of lactic acid, about 0.1% to 0.3% by weight of Zingiber officinale.

52. The method of claim 51, further comprising applying about 0.01% to 0.1% by weight of Ginger (Zingiber officinale) or an extract thereof, about 0.01% to 0.1% by weight of tetrasodium glutamic acid diacetate, about 0.01% to 0.1% by weight of diisopropyl adipate, about 0.01% to 0.1% by weight of triethyl citrate, about 0.01% to 0.1% by weight of sodium hydroxide, or about 0.01% to 0.1% by weight of benzyl alcohol.

53. 53. The composition of claim 51 or 52, further comprising applying an extract of Opuntia ficus-indica and / or an extract of Zingiber officinale to the scalp of a subject, wherein the extract of Opuntia ficus-indica and / or the extract of Zingiber officinale is a fruit extract, a leaf extract, a root extract, or a combination thereof.

54. 53. The composition of claim 51 or 52, further comprising applying an extract of Opuntia ficus-indica and / or an extract of Zingiber officinale to the scalp of a subject, wherein the extract of Opuntia ficus-indica and / or the extract of Zingiber officinale are whole plant extracts.

55. 55. The method according to any one of claims 51 to 54, wherein said application is a once-daily application.

56. 56. The method of any one of claims 51-55, further comprising rinsing the subject's scalp at least 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, or 48 hours after said application to the subject.

57. 57. The method of any one of claims 51-56, wherein: (a) the application is a first application; (b) the method results in an improvement in scalp hydration of at least 10% within about 1 month, about 2 months, or about 3 months of the first application; and (c) the improvement is measured using a corneometer.

58. 33. The method of any one of claims 29-32, wherein: (a) the application is a first application; (b) the method results in a reduction in sebum production in the scalp by at least 10% within about 1 month, about 2 months, or about 3 months of the first application; and (c) the reduction is measured using a sebometer.

59. The composition may comprise any one of sodium C14-16 olefin sulfonate, cetearyl alcohol, cocamidopropyl betaine, brassicamidpropyl dimethylamine, propanediol, sodium lauroyl sarcosinate, fragrance, sodium chloride, lactic acid, piroctone olamine, caprylyl / capryl glucoside, guar hydroxypropyltrimonium chloride, diisopropyl adipate, tetrasodium glutamate diacetate, glycol distearate, triethyl citrate, benzyl alcohol, sodium benzoate, potassium sorbate, cocamide MIPA, sodium hydroxide, hydroxypropyl methylcellulose, polyquaternium-37, isopropyl alcohol, citral, geraniol, caprylyl glyceryl ether, limonene, tetrasodium EDTA, linalool, citric acid, or CI 61891. The composition of any one of claims 1 to 6, 12 to 17, and 23 to 28, further comprising 77289 (chromium hydroxide green).

60. The composition may comprise any one of the following ingredients: sodium C14-16 olefin sulfonate, cetearyl alcohol, cocamidopropyl betaine, brassicamidpropyl dimethylamine, propanediol, sodium lauroyl sarcosinate, fragrance, sodium chloride, lactic acid, piroctone olamine, caprylyl / capryl glucoside, guar hydroxypropyltrimonium chloride, diisopropyl adipate, tetrasodium glutamate diacetate, glycol distearate, triethyl citrate, benzyl alcohol, sodium benzoate, potassium sorbate, cocamide MIPA, sodium hydroxide, hydroxypropyl methylcellulose, polyquatemium-37, isopropyl alcohol, citral, geraniol, caprylyl glyceryl ether, limonene, tetrasodium EDTA, linalool, citric acid, or CI 61891. The method of any one of claims 7 to 11 and 18 to 22, further comprising 77289 (chromium hydroxide green).

61. The subject's scalp may be treated with one or more of the following: sodium C14-16 olefin sulfonate, cetearyl alcohol, cocamidopropyl betaine, brassicamidpropyl dimethylamine, propanediol, sodium lauroyl sarcosinate, fragrance, sodium chloride, lactic acid, piroctone olamine, caprylyl / capryl glucoside, guar hydroxypropyltrimonium chloride, diisopropyl adipate, tetrasodium glutamate diacetate, glycol distearate, triethyl citrate, benzyl alcohol, sodium benzoate, potassium sorbate, cocamide MIPA, sodium hydroxide, hydroxypropyl methylcellulose, polyquaternium-37, isopropyl alcohol, citral, geraniol, caprylyl glyceryl ether, limonene, tetrasodium EDTA, linalool, citric acid, or CI 618101.

59. The method of any one of claims 29 to 58, further comprising applying 77289 (chromium hydroxide green).

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