Cell culture system, its use, and cells derived therefrom
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- UNIV OF PITTSBURGH OF THE COMMONWEALTH SYST OF HIGHER EDUCATION
- Filing Date
- 2023-05-24
- Publication Date
- 2026-06-03
AI Technical Summary
Current in vitro models for human embryonic development suffer from low efficiency, limited scalability, and high technical complexity, and lack systems that can support the integrated morphogenetic events of post-implantation human embryos, particularly those involving germ and extraembryonic layers.
An in vitro or ex vivo cell culture system is developed using stem cell populations with inducible transgenes for GATA6 and optionally ETV2 polypeptides, which are induced to form cell aggregates comprising embryonic and extraembryonic structures, such as bilayer disk-like structures, amnion-like domains, and yolk sac domains, through a self-organizing process.
The system effectively generates cell aggregates that mimic early human embryonic development, allowing for the study of embryonic and hematopoietic differentiation, and can be used to evaluate therapeutic agents, with reproducible formation of distinct cell structures without additional reagents.
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