Epidermal thinning inhibitor and epidermal barrier function improver

Abstract

To provide a skin care preparation containing an active ingredient which is derived from a natural product, is excellent in biological safety, suppresses thinning of epidermis, and exhibits an effect of improving an epidermal barrier function.SOLUTION: The composition contains a mixture of a fermentation product of adlay seeds, a Salicornia europaea extract and an Aspalathus linearis extract as an active ingredient.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an agent for inhibiting epidermal thinning and an agent for improving epidermal barrier function, which contain multiple plant-derived components. [Background technology]

[0002] It is known that epidermal function declines when the epidermis is damaged by factors such as ultraviolet rays, air pollutants, and dryness, resulting in a decrease in epidermal cell activity and disruption of cell turnover. Conventionally, various moisturizers (amino acids, glycerin, polyhydric alcohols, sodium lactate, etc.) have been proposed as ingredients to protect the epidermis, but they have had issues with stability and effectiveness. DISCLOSURE OF THE INVENTION [Problem to be solved by the invention]

[0003] In view of the problems of the prior art, the present inventors have discovered a problem in which epidermal thinning occurs due to decreased epidermal cell activity and disrupted turnover, and have conducted extensive research into new active ingredients derived from natural products that can solve this problem. As a result, they have found that a combination of a fermented product of Job's tears seeds, a Salicornia herb extract, and an Aspalathus linearis extract has the effect of suppressing epidermal thinning and improving the epidermal barrier function.

[0004] It has been disclosed in Patent Documents 1 to 3, for example, that each of fermented Job's tears seeds, Salicornia herbacea extract, and Aspalathus linearis extract has skin physiological activity. However, it was not known that a combination of these fermented products and extracts has the effect of suppressing epidermal thinning. [Patent Document 1] Japanese Patent Application Laid-Open No. 2005-145878 [Patent Document 2] Japanese Patent Application Laid-Open No. 2010-138139 [Patent Document 3] Japanese Patent Application Laid-Open No. 2009-249371 [Means for solving the problem]

[0005] The present invention provides an epidermal thinning inhibitor comprising a mixture of fermented Job's tears seeds, Salicornia herbacea extract, and Aspalathus linearis extract (solid concentration ratio of fermented Job's tears seeds:Salicornia herbacea extract:Aspalathus linearis extract=5.5-6.0:3.0-3.5:1). The present invention also provides an epidermal barrier function improver comprising a mixture of fermented Job's tears seeds, Salicornia herbacea extract, and Aspalathus linearis extract (solid concentration ratio of fermented Job's tears seeds:Salicornia herbacea extract:Aspalathus linearis extract=5.5-6.0:3.0-3.5:1). The present invention also relates to an external skin preparation containing the above-mentioned agent for inhibiting epidermal thinning or the above-mentioned agent for improving epidermal barrier function. [Effects of the Invention]

[0006] The present invention provides an external skin preparation that inhibits epidermal thinning and, as a result, improves the barrier function of the epidermis by mixing a combination of fermented Job's tears seeds, Salicornia herbacea extract, and Aspalathus linearis extract in a specific blending ratio to form active ingredients. [Brief explanation of the drawings]

[0007] [Figure 1] FIG. 1 is a graph showing the effect of the extract according to the present invention in inhibiting epidermal thinning. DETAILED DESCRIPTION OF THE INVENTION

[0008] In the present invention, "jobi's tears" refers to a plant of the genus Job's tears in the family Poaceae, and may be any species. The part used for fermentation is preferably the seeds, and either those with or without the husks can be used. Furthermore, the seeds may be whole grains, or may be powder obtained by crushing or crushing, or Job's tears seed grains or powder that have been treated at high temperature and under high pressure.

[0009] Yeast is preferably used as the fungus used for fermenting Job's tears, and preferred yeasts are those of the genus Saccharomyces, such as Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, and Saccharomyces bayonus.

[0010] The Salicornia used in the present invention is a plant of the genus Salicornia in the family Chenopodiaceae, and is sometimes called coral grass. Examples include Salicornia europaea, Salicornia bigelowii, and Salicornia dolichostachia ssp. strictissima. The whole plant is preferably used for extraction.

[0011] The Aspalathus linearis used as the raw material for the Aspalathus linearis extract of the present invention is a plant belonging to the genus Aspalathus in the family Fabaceae, and is preferably non-fermented and green (so-called green rooibos).The part used for extraction is preferably the whole plant or leaves.

[0012] To prepare the extract, first, the part of each plant to be used is washed with water if necessary to remove foreign matter, and then, either as is or dried, is shredded or crushed as necessary, and then brought into contact with an extracting solvent for extraction. Extraction can be carried out by contacting with the extracting solvent according to a conventional method such as the immersion method.

[0013] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these may be used alone or in combination.

[0014] Among the above-mentioned extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention from the viewpoints of the efficacy of the resulting extract, skin irritation, and the wide applicability of these solvents to topical skin preparations (cosmetics, quasi-drugs, etc.). Preferred examples of hydrophilic solvents include the use of water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol) alone, or a mixed solvent of water and a lower alcohol (particularly ethanol), or a mixed solvent of water and a polyhydric alcohol (particularly 1,3-butylene glycol, glycerin). Among these, water alone or a mixed solvent of water and 1,3-butylene glycol is particularly preferred.

[0015] When a mixed solvent is used, the mixing ratio is preferably in the range of, for example, a volume ratio (same hereinafter) of 1:99 to 99:1 for a mixed solvent of water and 1,3-butylene glycol, 1:25 to 25:1 for a mixed solvent of water and ethanol, or 1:20 to 20:1 for a mixed solvent of water and glycerin.

[0016] The weight ratio of the part of each plant to be used to the extraction solvent is generally 1:1 to 1:50.

[0017] When preparing the extract solution, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, the extract solvent may be blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid to adjust the pH to the desired level.

[0018] The extraction conditions, such as extraction temperature and extraction time, vary depending on the type and pH of the solvent used. For example, when water, 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent, the extraction temperature is preferably in the range of 0°C to 80°C, more preferably in the range of 0°C to 20°C, and the extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week), more preferably in the range of 1 to 120 hours (1 hour to 5 days).

[0019] The extract solutions prepared as described above are mixed and generally adjusted to a pH of 3 to 8. The mixture may be used as an ingredient in a topical skin preparation as is, or may be used at a desired concentration by vacuum concentration, etc. The extract may also be dried by a conventional method such as spray drying.

[0020] The solids concentration of the Job's tears fermented solution according to the present invention is preferably 0.1 to 5.0% by weight in the fermented solution. The solids concentration of the Salicornia extract solution according to the present invention is preferably 0.1 to 5.0% by weight in the extract, and the solids concentration of the Aspalathus linearis extract solution according to the present invention is preferably 0.1 to 5.0% by weight in the extract.

[0021] When the fermented product and extract according to the present invention are incorporated into an external skin preparation, the solid content concentration is preferably 0.001 to 10% by weight or more.

[0022] Examples of topical skin preparations (cosmetics, quasi-drugs, etc.) containing the composition of the present invention include cleansing cosmetics such as emulsions, creams, lotions, essences, transparent gels, packs, lipsticks, foundations, sheet masks, liquid foundations, makeup press powders, blushers, face powders, facial cleansers, body shampoos, hair shampoos, and soaps.

[0023] When the fermented product and extract according to the present invention are incorporated into topical skin preparations (cosmetics, quasi-drugs, etc.), ingredients used in topical skin preparations, such as oily ingredients, surfactants (synthetic or natural), moisturizers, thickeners, emulsifiers or emulsifier aids, preservatives / bactericides, powder ingredients, UV absorbers, antioxidants, pigments, fragrances, anti-wrinkle agents, and other physiologically active ingredients, can be appropriately incorporated as needed. Furthermore, as long as the efficacy and features of the composition according to the present invention are not impaired, there is no problem in incorporating the fermented product and extract into topical skin preparations in combination with other physiologically active ingredients.

[0024] Examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, and other plant-derived oils and fats such as squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, paraffin wax, Examples include hydrocarbons such as squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; and synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0025] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of surfactants that can be used include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0026] Examples of emulsifiers and / or emulsifying aids that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or derivatives thereof, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble polysaccharides, soybean-derived protein and polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc.

[0027] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid or its salts or derivatives, hyaluronic acid hydrolyzate, chondroitin or its derivatives, heparin or its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, lactic acid, urea, and higher fatty acid octyldodecyl.

[0028] Examples of thickeners include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

[0029] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or a derivative thereof.

[0030] Examples of antiseptics and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, alkylisoquinolinium bromide, resorcinol, jamal (imidazoline urea), isopropylmethylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, hexanediol, concentrated benzalkonium chloride solution 50, and essential oils such as peppermint oil and eucalyptus oil.

[0031] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0032] Anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0033] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybean, adzuki bean, etc.), etc.

[0034] Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0035] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.), and the like.

[0036] Further, examples of whitening agents include one or more selected from ellagic acid and its derivatives, resorcinol derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate).

[0037] Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol; examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid; and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. Examples include one or more selected from kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, alpha-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acid derivatives, mulberry bark extract, chamomile extract, hydrolyzed rice bran extract, saxifrage extract, and white mustard extract or hydrolyzates thereof.

[0038] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid ester salts such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate; Ascorbic acid sugar derivatives such as ascorbic acid 2-glucoside, L-ascorbic acid 5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, etc.; 6-acylated products of these ascorbic acid sugar derivatives (the acyl group is hexanoyl, octanoyl, decanoyl, etc.); L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, etc.; L-ascorbic acid tetrafatty acid esters such as 3-isopalmitate, ... -O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, L-ascorbic acid aminopropyl phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc. Hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), Examples of tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), and tranexamic acid amides (e.g., tranexamic acid methylamide). Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid,Examples of nicotinic acid derivatives include nicotinamide (niacinamide) and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0039] Anti-wrinkle agents include vitamin A or its derivatives, niacinamide, vitamin E or its derivatives (tocopherol acetate, etc.), vitamin C or its derivatives (ascorbic acid glucoside, 3-O-ethyl ascorbic acid, ascorbic acid phosphate magnesium salt, etc.), pantothenyl alcohol, tranexamic acid, etc.

[0040] Furthermore, it is also possible to use in combination components derived from natural products such as the following plants or microorganisms: collagen or hydrolysates thereof, yeast extracts or hydrolysates, lactic acid bacteria cultures, grasses, cruciferous plants, Theaceae plants, Rosaceae plants, Paeoniaceae plants, Rutaceae plants, Amaranthaceae plants, Zosteraea plants, Leguminosae plants, Asteraceae plants, Fabaceae plants, Malvaceae plants, Gentianaceae plants, Lamiaceae plants, Nelumbosacaceae plants, Cucurbitaceae plants, Araliaceae plants, Solanaceae plants, Bignoniaceae plants, Actinidiaceae plants, Mulberry plants, Iridaceae plants, Campanulaceae plants, Oleaceae plants, Actinidiaceae plants, Mulberry plants, Rhamnaceae plants, Orchidaceae plants, and Anacardiaceae plants. Examples of suitable extracts include extracts of one or more plants selected from the family Garcinia, Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae, or hydrolysates or fermented products thereof; extracts of one or more seaweeds selected from the family Laminaria, Mirrataceae, and Ulvulaceae, or hydrolysates or fermented products thereof; jellyfish (autolyzed products of moon jellyfish, Nomura's jellyfish, etc.); hydrolysates or fermented products of hyaluronic acid; and extracts of royal jelly, or hydrolysates or fermented products thereof.

[0041] The present invention will now be described in more detail with reference to Production Examples, Test Examples, and Formulation Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all % mean % by weight.

[0042] Production Example 1. Preparation of fermented Job's tears solution 50 g of husk-removed Job's tears seeds were crushed and 950 g of purified water was added to prepare a suspension, which was then heat-sterilized. 7 The cells / mL were inoculated and statically cultured for 3 days at 37° C. After the culture was completed, the mixture was sterilized by heating, cooled to room temperature, and filtered to obtain 500 g of a fermented Job's tears seed solution (solid concentration 1.01%).

[0043] Preparation Example 2: Preparation of Salicornia herb extract solution 200 g of purified water was added to 20 g of dried, shredded Salicornia herbacea whole plant, and the mixture was extracted for 1 hour at 40° C. The resulting extract was filtered to obtain 155 g of a brown, transparent extract solution (solid content: 1.98%).

[0044] Preparation Example 3. Preparation of Aspalathus linearis extract solution 100g of dried whole non-fermented rooibos from Aspalathus linearis, a plant of the Aspalathus genus of the Leguminosae family, was added to 1000g of a mixed solvent of purified water and 1,3-butylene glycol, and extracted at 80°C for 2 hours. After removing insoluble matter by filtration, 1028g of dark brown green rooibos extract (solid concentration 1.22%) was obtained.

[0045] The fermented product solution of Production Example 1 and the extract solutions of Production Examples 2 and 3 prepared as above were mixed to an optimal solid content concentration ratio, i.e., Job's tears fermented product solution (Production Example 1): Salicornia extract solution (Production Example 2): Aspalathus linearis extract solution (Production Example 3) = 5.5-6.0:3.0-3.5:1, as described above, and this mixture was used as the composition of the present invention (Composition 1). Specifically, for example, the solid content concentration ratio can be adjusted by mixing Job's tears fermented product solution (Production Example 1): Salicornia extract solution (Production Example 2): Aspalathus linearis extract solution (Production Example 3) in the blending ratio described in the formulation examples described below.

[0046] Test Example 1: Evaluation test of epidermal thickness Culture of a three-dimensional skin model, LabCyte Epi-Model 6D (J-tec), was initiated according to the attached protocol. After 24 hours, the sample solution was added to the stratum corneum. In Test Example 1, the above-mentioned Composition 1 (1.0%) was prepared and used as the sample solution. The addition treatment was performed every three days, and the culture was completed after eight days from the first addition treatment. After the culture was completed, the three-dimensional skin model was fixed, sectioned, and stained with hematoxylin-eosin (HE) for observation. The thickness of the stratum corneum and viable cell layer was measured using image analysis software (ImageJ). In parallel, a control group was set up in which a control solution (30BG) was added instead of the sample solution, and the same procedure was performed.

[0047] The results of Test Example 1 confirmed that in the test group to which the sample solution was added, the thickness of the viable cell layer in the three-dimensional skin model was approximately 1.3 times that of the control group, and the thickness of the stratum corneum was approximately 1.9 times that of the control group. In other words, it was confirmed that applying Composition 1 of the present invention, which is a mixture of the fermented product of Production Example 1, the extract of Production Example 2, and the extract of Production Example 3, to the skin inhibits epidermal thinning. This suggests the effect of guiding the epidermis to a fully differentiated state and improving barrier function.

[0048] Below, examples of formulations for skin external preparations containing the fermented products and extracts according to the present invention, such as lotions, emulsions, and creams, are shown, but the present invention is not limited to these.

[0049] Prescription example 1. Lotion [Ingredients] Part Jojoba oil 1.0 Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Glycerin 5.0 Niacinamide 5.0 Dipotassium glycyrrhizinate 0.1 1,3-butylene glycol 1.0 Water-soluble collagen 0.01 Propanediol 3.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0050] Prescription example 2: Lotion [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Squalane 1.0 Glycerin 5.0 Niacinamide 5.0 Tranexamic acid 2.0 1,3-butylene glycol 1.0 Hydrolyzed Collagen 0.1 Pentanediol 3.0 Sodium citrate (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0051] Prescription example 3: Lotion [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Hydrogenated castor oil 1.0 Glycerin 5.0 L-Ascorbic Acid 2-Glucosyl 2.0 Tocopheryl acetate 0.3 Water-soluble collagen 0.01 1,3-butylene glycol 1.0 Pentanediol 3.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0052] Prescription example 4: Emulsion [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Squalane 5.0 Jojoba oil 1.0 Orange Peel Oil 1.0 Polyoxyethylene (20) Sorbitan Monostearate 1.0 Lipophilic glyceryl stearate 1.0 Hydrogenated soy lecithin 1.5 Niacinamide 5.0 D-Pantothenyl alcohol 0.1 Glycerin 3.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Sodium hyaluronate 0.01 1,3-butylene glycol 0.1 Phenoxyethanol 0.5 Pentylene glycol 0.5 Diglycerin 0.3 Purified water (enough to make the total volume 100 parts)

[0053] Prescription example 5. Emulsion [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Squalane 5.0 Castor oil 1.0 Lipophilic glyceryl stearate 1.0 Hydrogenated soy lecithin 1.5 Niacinamide 5.0 Tranexamic acid 2.0 Glycerin 3.0 Carboxymethylcellulose 0.3 Sodium hyaluronate 0.01 Hydrolyzed Hyaluronic Acid 0.01 1,3-butylene glycol 0.1 Phenoxyethanol 0.5 Pentylene glycol 0.5 Diglycerin 0.3 Purified water (enough to make the total volume 100 parts)

[0054] Prescription example 6. Cream [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Niacinamide 5.0 Tranexamic acid 2.0 D-Pantothenyl alcohol 0.1 Dipotassium glycyrrhizinate 0.1 Lactic acid bacteria fermented rice 2.0 Hydrogenated lecithin 0.5 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Water-soluble collagen 0.1 1,3-butylene glycol 0.1 Phenoxyethanol 0.5 Pentylene glycol 0.5 Diglycerin 0.3 Purified water (enough to make the total volume 100 parts)

[0055] Prescription example 7. Facial cleanser [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Dipotassium glycyrrhizinate 0.1 Glyceryl Stearate 2.5 Squalane 3.0 Glycerin 3.0 Polyglyceryl-10 Laurate 3.0 Water-soluble collagen 0.01 Sodium hyaluronate 0.01 Isopropylmethylphenol 0.1 1,3-butylene glycol 2.0 Purified water (enough to make the total volume 100 parts)

Claims

1. An epidermal thinning inhibitor comprising a mixture of fermented Job's tears seeds, Salicornia herbacea extract, and Aspalathus linearis extract (solid concentration ratio of fermented Job's tears seeds:Salicornia herbacea extract:Aspalathus linearis extract=5.5-6.0:3.0-3.5:1).

2. An epidermal barrier function improver comprising a mixture of fermented Job's tears seeds, Salicornia herbacea extract, and Aspalathus linearis extract (solid concentration ratio of fermented Job's tears seeds:Salicornia herbacea extract:Aspalathus linearis extract=5.5-6.0:3.0-3.5:1).

3. A skin external preparation containing the epidermal thinning inhibitor according to claim 1.

4. A skin external preparation containing the epidermal barrier function improver according to claim 2.

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