lettuce plants

Abstract

To provide a new lettuce variety. [Solution] The present invention provides seeds, plants and tissue cultures of lettuce plants of lettuce line TEXLE213131, identified by accession number FERM BP-22497, as well as methods for producing lettuce plants produced by crossing said lettuce plants with any other lettuce plants.

Description

[Technical Field]

[0001] The present invention relates to lettuce plants. [Background technology]

[0002] Lettuce plants prefer cool climates. In recent years, they have been suffering from root rot and other diseases, as well as poor growth, which are thought to be caused by continuous cropping and abnormal weather, such as high temperatures and humidity. Producers are seeking varieties that can be produced stably. [Prior art documents] [Non-patent literature]

[0003] [Non-Patent Document 1] M. FUJINAGA, ``Studies on physiological races and phylogenetic analysis of lettuce root rot pathogen, Fusarium oxysporum f. sp. Lactucae'', Journal of General Plant Pathology volume 71, page457 (2005) [Non-patent document 2] T. SHIMIZU et.al., “Development Trend of Diseases on Lettuce (Lactuca sativa) of Organic Cultivation in Highlands of Nagano Prefecture and Their Control”, Annual report of the Kanto-Tosan Plant Protection Society 64:2017.12 p.41-46 Summary of the Invention [Problem to be solved by the invention]

[0004] Therefore, an object of the present invention is to provide a new lettuce plant. [Means for solving the problem]

[0005] In order to achieve the above object, the present invention provides a lettuce plant and its seeds, TEXLE213131, and a representative seed thereof has been deposited under accession number FERM BP-22497.

[0006] Lettuce plants of the present invention are grown from seeds of TEXLE213131 or seeds identified by accession number FERM BP-22497.

[0007] The method for producing a lettuce plant of the present invention includes a selfing step of selfing the lettuce plant of the present invention.

[0008] The method for producing a lettuce plant of the present invention includes a hybridization step of crossing the lettuce plant of the present invention with another lettuce plant. [Effects of the Invention]

[0009] According to the present invention, a new lettuce plant can be provided. [Brief explanation of the drawings]

[0010] [Figure 1] FIG. 1 is a schematic diagram showing an example of leaf-top overlap and leaf lobe number on a lettuce plant. [Figure 2] FIG. 2 is a schematic diagram showing an example of the leaf shape of a lettuce plant. [Figure 3] FIG. 3 is a schematic diagram showing examples of the depth of the marginal notch in the leaf of a lettuce plant, the size of the bulb, and the shape of the vertical cross section of the bulb. DETAILED DESCRIPTION OF THE INVENTION

[0011] <Lettuce plants> The lettuce plant of the present invention includes a lettuce plant (hereinafter also referred to as "deposited strain") represented by TEXLE213131 and identified by the accession number FERM BP-22497 (deposited) or its progeny strain. The lettuce plant of the present invention is characterized by including a lettuce plant represented by TEXLE213131 and identified by the accession number FERM BP-22497 or its progeny strain, and may include new mutations, transgenes and / or single locus conversions, and other configurations and conditions are not particularly limited.

[0012] In the present invention, the "lettuce plant" is a plant classified in the genus Lactuca of the family Asteraceae, species Lactuca sativa L., with the scientific name Lactuca sativa and the Japanese name Chisha (Lettuce, Lactuca, Chisa). The lettuce plant may be, for example, a hybrid with a related species or a wild species.

[0013] In the present invention, the "cultivated lettuce plant", "cultivated lettuce variety", "cultivated lettuce strain", or "cultivated lettuce" is a lettuce plant or its variety, breeding strain or cultivated variety that is cultivated by humans and is excellent in cultivation science. The "cultivated lettuce plant", "cultivated lettuce variety", "cultivated lettuce strain" or "cultivated lettuce" may be their hybrids or hybrids with other lettuce species.

[0014] In the present invention, the "plant" means a plant individual representing the whole plant.

[0015] In the present invention, "plant part" refers to a part of an individual plant. Examples of the "plant part" include plant cells, plant protoplasts, plant cell cultures or tissue cultures capable of regenerating plants, plant callus, plant clumps, plant cells isolated from plants or plant parts, leaves, heads, expanded leaves, pollen, embryos, cotyledons, hypocotyls, roots, root tips (root tips), anthers, pistils, flowers, ovaries, ovules, seeds, fruits, stems, and seedlings. Examples of the part of an individual plant include organs, tissues, cells, and vegetative propagules, and any of these may be used. Examples of the organs include petals, corollas, flowers, leaves, seeds, fruits, stems, and roots. Examples of the tissues are parts of the organs. Specific examples of the part of a plant include a microspore, flower, flower bud, pistil, anther, pollen, ovary, embryo, ovule, hypocotyl, embryo sac, egg cell, cutting, root, root tip, stem, stalk, leaf, head, expanded leaf, petiole, leaf pith, cotyledon, cell, meristematic cell, protoplast, and seed. The pollen may be mature or immature pollen. The part of a plant may be derived from any growth stage of a plant, such as pre-rooting, post-rooting, seedling, cutting, or mature plant. The part of a plant may be, for example, one type of organ, tissue, and / or cell, or two or more types of organs, tissues, and / or cells.

[0016] In the present invention, "root rot (lettuce root rot)" refers to a disease caused by a filamentous fungus. Examples of pathogenic fungi that cause root rot include Fusarium oxysporum f. sp. Lactucae. Multiple races of the fungus have been reported to cause the disease, including race 1, race 2, and race Yuma. The term "race" refers to fungal strains with different pathogenicity, more specifically, strains that exhibit different pathogenicity against varieties with different resistance genes or loci. "Race Yuma" refers to a fungus that can be isolated from soil in Yuma, Arizona, USA, and that causes lettuce root rot on the lettuce varieties Costa Rica 4 and late-flowering Red Fire.

[0017] In the present invention, "resistance" is also referred to as, for example, "disease resistance" or "tolerance." The resistance means, for example, the ability to inhibit or suppress the occurrence and progression of a disease caused by infection with a pathogen, and specifically may mean, for example, the absence of the disease, the cessation of the progression of an already occurring disease, or the suppression (also referred to as "inhibition") of the progression of an already occurring disease.

[0018] In the present invention, the "root rot resistance" can be evaluated in accordance with the measurement method for trait number 59 described below. In the present invention, lettuce plants exhibiting the "root rot resistance" may be resistant to any one or more races, and may be resistant to multiple races. Preferably, they have resistance to race 1 of root rot fungus and / or race Yuma of root rot fungus.

[0019] <Deposited strain> The lettuce plant of the present invention is designated TEXLE213131, and a representative example thereof is the lettuce plant identified by accession number FERM BP-22497 or its progeny line. Hereinafter, accession number FERM BP-22497 will also be referred to as lettuce line TEXLE213131. Information regarding the deposit of the variety is provided below.

[0020] (TEXLE213131) Type of deposit: International deposit Name of depository institution: National Institute of Technology and Evaluation, Patent Organism Depositary Center Address: Room 120, 2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture, 292-0818, Japan Accession number: FERM BP-22497 Identification mark: TEXLE213131 Received: May 16, 2024

[0021] The deposited line exhibits, for example, the morphological and physiological characteristics and resistance to the root rot fungus race Yuma listed in Table 1 below. The morphological and physiological characteristics are based on cultivation in 2023 at the Shiojiri experimental site of Takii Seed Nagano Research Farm, Shiojiri City, Nagano Prefecture. The morphological and physiological characteristics in Table 1 below are evaluated based on the Lettuce Variety Evaluation Criteria published by the Ministry of Agriculture, Forestry and Fisheries of Japan (MAFF) (published in September 2022, https: / / www.maff.go.jp / j / shokusan / hinshu / info / kijun / 1361.pdf). The morphological and physiological characteristics in Table 1 below are evaluated based on the criteria described below. For the morphological and physiological characteristics, see Figures 1 to 3. The evaluation of the morphological and physiological characteristics may also refer to the DUS test guidelines in UPOV. As the DUS test guidelines, for example, the guidelines published on April 5, 2017 (https: / / www.upov.int / edocs / tgdocs / en / tg013.pdf) can be referred to.

[0022] [Table 1]

[0023] (Trait number 1) The "seed color" can be evaluated by visual observation, using grades 1 (white, standard variety: Olympia), 2 (yellow), 3 (brown, standard varieties: Cisco, V lettuce), and 4 (black, standard variety: Logic) as standards.

[0024] (Trait number 4) The "plant width" refers to the maximum diameter (cm) of the plant and can be evaluated by measurement. The "plant width" can be evaluated based on the following criteria: Class 1 (very small), Class 3 (small, standard variety: Okayama lettuce), Class 5 (medium, standard variety: Olympia), Class 7 (large), and Class 9 (very large).

[0025] (Trait number 5) The "overlap of the upper parts of the leaves of the plant" refers to the degree of overlap of the upper parts of the leaves of the plant (degree of head formation) and can be evaluated by visual observation. The "overlap of the upper parts of the leaves of the plant" can be evaluated based on the following criteria: Grade 1 (none or weak, standard variety: Prizehead), Grade 2 (medium, standard variety: Costa Rica No. 4), and Grade 3 (strong, standard varieties: Olympia and Vanguard 75).

[0026] (Trait number 8) The "number of leaf lobes" refers to the number of leaf lobes and can be evaluated by visual observation. The "number of leaf lobes" can be evaluated based on the following criteria: grade 1 (none or very small, standard variety: Olympia), grade 3 (few), grade 5 (medium), grade 7 (many), and grade 9 (very many).

[0027] (Trait number 9) The "leaf shape" refers to the leaf shape of cultivars with no or very few leaf lobes (limited to cultivars with no or very few leaf lobes), and can be evaluated by visual observation. The "leaf shape" can be evaluated based on the following criteria: grade 1 (triangular), grade 2 (lanceolate), grade 3 (oblate, standard cultivar: Olympia), grade 4 (narrow oblate), grade 5 (round, standard cultivar: White Boston), grade 6 (broad elliptical), grade 7 (elliptical, standard cultivar: Costa Rica No. 4), grade 8 (narrow elliptical, standard cultivar: Red Persimmon), grade 9 (linear), grade 10 (broad obovate), grade 11 (obovate, standard cultivar: Prizehead), and grade 12 (oblanceolate).

[0028] (Trait number 13) The "anthocyanin coloration of leaves" refers to the intensity of anthocyanin coloration of leaves, and can be evaluated by visual observation. The "anthocyanin coloration of leaves" can be evaluated based on the following criteria: Grade 1 (none or very weak), Grade 3 (weak), Grade 5 (medium, standard variety: Prizehead), Grade 7 (strong), and Grade 9 (very strong).

[0029] (Trait number 22) The "leaf unevenness" refers to the strength of the unevenness (shrinkage) of the leaf surface and can be evaluated by visual observation. The "leaf unevenness" can be evaluated based on the following criteria: grade 1 (none or very weak, standard variety: Cisco), grade 3 (weak, standard variety: Olympia), grade 5 (medium, standard variety: Early Impulse), grade 7 (strong, standard variety: Nishinabeni), and grade 9 (very strong, standard variety: Black Seeded Simson).

[0030] (Trait number 24) The "waviness of the leaf margin" refers to the strength of the waviness of the leaf margin and can be evaluated by visual observation. The "waviness of the leaf margin" can be evaluated as grade 1 (none or very weak), grade 3 (weak), grade 5 (medium, standard variety: Mikado Great 3204), grade 7 (strong, standard variety: Kalmar, Grand Rapid), or grade 9 (very strong).

[0031] (Trait number 26) The "depth of the notches at the margins of the leaves" refers to the depth of the notches at the margins of the leaves and can be evaluated by visual observation. The "depth of the notches at the margins of the leaves" can be evaluated based on the following criteria: grade 1 (none or very shallow), grade 3 (shallow), grade 5 (medium, standard variety: Olympia), grade 7 (deep), and grade 9 (very deep).

[0032] (Trait number 30) The "bulb size" refers to the size of the bulb, and refers to the bulb size (bulb diameter x bulb height) of varieties with medium or strong overlap of the upper leaves of the plant (limited to varieties with medium or strong overlap of the upper leaves of the plant), and can be evaluated by measurement and visual observation. The "bulb size" can be evaluated based on the following criteria: Class 1 (very small), Class 3 (small), Class 5 (medium, standard variety: Olympia), Class 7 (large), and Class 9 (very large).

[0033] (Trait number 31) The "shape of the bulb in longitudinal section" refers to the shape of the bulb in longitudinal section, and refers to the shape of the bulb in longitudinal section of a variety with medium or strong overlap of the upper leaves of the plant (limited to varieties with medium or strong overlap of the upper leaves of the plant), and can be evaluated by visual observation. The "shape of the bulb in longitudinal section" can be evaluated based on the following criteria: Class 1 (narrow oval), Class 2 (wide oval), Class 3 (circular, standard variety: Excelheadgrass), and Class 4 (oblate, standard variety: Cisco).

[0034] (Trait number 40) The "initiation of bolting" refers to the timing of bolting initiation under long-day conditions (the time when 50% of the test plants have bolted is the number of days from the sowing date), and can be evaluated by measurement and visual observation. The "initiation of bolting" can be evaluated based on the following criteria: Class 1 (very early, standard variety: Green Leaf), Class 3 (early, standard variety: Prize Head), Class 5 (medium, standard varieties: Gett and Falcon), Class 7 (late, standard variety: Olympia), and Class 9 (very late).

[0035] (Trait number 59) The term "resistance to root rot race 1" refers to the degree of resistance to root rot race 1. The "resistance to root rot race 1" can be evaluated based on the following criteria: grade 1 (susceptible, standard varieties: Patriot, late-flowering Red Fire, Waldman's Green), grade 2 (moderate resistance, standard variety: Salinas 88), and grade 3 (high resistance, standard variety: Costa Rica No. 4).

[0036] The resistance to race 1 of root rot fungus can be confirmed by the following test method. Testing Method Pathogen: Lettuce root rot fungus (Fusarium oxysporum f. sp. lactucae (Fol)) Race 1 (Race 1) is used. The race is confirmed using susceptible varieties. Pathogenicity confirmation: Pathogenicity is confirmed using susceptible varieties before testing. · Testing Number of test individuals: At least 30 individuals, divided into two or more replicates (for non-inoculated areas, at least 20 individuals, divided into two or more replicates). Standard varieties: Susceptible; Patriot, late-flowering Red Fire, Waldman's Green; moderately resistant; Salinas 88; and highly resistant; Costa Rica No. 4. Temperature: Generally, keep the temperature within the range of 20°C at night and 25-28°C during the day. Illumination: Natural light or at least 15,000lx, 14 hours of light. Inoculation preparation: After inoculating the test strain cultivated in a nutrient medium (PSA medium, etc.) into the bran medium, mix and cultivate at approximately 25°C for 10 to 14 days. The medium in which the bacteria have grown sufficiently throughout the medium is used as the inoculum. Inoculation method: Add the inoculum to sterilized soil (or commercially available potting soil) at a volume ratio of 20:1 and mix well. Fill pots with this as contaminated soil, and sow healthy seeds after germination. If the seeds have poor germination vigor, it will be difficult to determine whether they have died due to disease, so try to avoid sowing seeds with poor germination vigor. Test period: The degree of disease is evaluated approximately 20 to 30 days after inoculation. Judgment criteria: Evaluate the degree of disease for each individual as follows, and calculate the disease index to make a judgment. Disease severity 0: No symptoms Severity 1: Mild stunting and growth suppression Severity 2: Severe stunting Disease severity 3: Death Disease index = Σ (disease level × number of individuals with each disease level) / (total number of individuals) Judgment: Evaluate by comparing with the disease index of each standard variety. In addition to the above test methods, the seedling immersion method can also be used. For details of the seedling immersion method, see, for example, the UPOV Test Guideline (LETTUCE; TG / 13 / 11 Rev.2, https: / / www.upov.int / edocs / tgdocs / en / tg013.pdf).

[0037] The deposited line may have "resistance to the root rot fungus race Yuma" in addition to the morphological and physiological characteristics listed in Table 1. The "resistance to the root rot fungus race Yuma" can be evaluated based on whether the line is resistant (resistant variety: Meridian) or not (susceptible variety: Patriot).

[0038] The presence or absence of resistance to the root rot fungus race Yuma can be confirmed by using race Yuma collected from soil in Yuma, Arizona, USA as a pathogen and applying the above-mentioned method for testing resistance to root rot fungus race 1.

[0039] In the present invention, a plant having "essentially all physiological and morphological characteristics of the deposited line" means a plant that has the major traits of the deposited line from which the target plant is derived when grown in the same environment. The major traits are traits (1) to (14) below, i.e., trait numbers 40, 59, 1, 4, 5, 8, 9, 13, 22, 24, 26, 30, and 31 in Table 1, and resistance to the root rot fungus race Yuma. The major traits are preferably traits 40 and 59 in Table 1, and resistance to the root rot fungus race Yuma, i.e., traits (1), (2), and (14) below. The plant having "essentially all physiological and morphological characteristics of the deposited line" may be a plant having the same traits as the deposited line except for, for example, 14 or less, 13 or less, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait. That is, the plant may have 14 or less, 13 or less, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait. The "traits different from those of the deposited line" may be major traits of the deposited line or traits other than the major traits of the deposited line, but traits other than the major traits of the deposited line are preferred. The "traits different from those of the deposited line" can be achieved, for example, by introducing traits and / or genes as described below. In plants having "essentially all of the physiological and morphological characteristics of the deposited line," all of the trait numbers 40, 59, 1, 4, 5, 8, 9, 13, 22, 24, 26, 30, and 31, and the trait for resistance to root rot fungus race Yuma, may be the same as those of the deposited line. (1) The start of bolting is "slightly late"; (2) resistance to root rot race 1 is "highly resistant"; (3) The seed color is "white"; (4) The width of the stock is "medium"; (5) The overlap of the upper leaves of the plant is "strong"; (6) The number of leaf lobes is "none or very small"; (7) The leaf shape is "narrow oblate"; (8) Anthocyanin coloration of the leaves is "absent or very weak"; (9) The leaf irregularities are "slightly strong"; (10) The leaf margins are medium in undulation; (11) The depth of the incision on the margin of the leaf is medium; (12) The size of the ball is "medium"; (13) The leaf cross section is "oblate"; (14) Resistance to the root rot fungus race Yuma is present.

[0040] <Progeny lineage> The lettuce plant of the present invention may be a progeny line of the deposited line. The progeny line may be an individual plant of the progeny line, a part of an individual plant of the progeny line, or a seed of the progeny line.

[0041] In the present invention, a "progeny line" or "progeny lettuce plant" (hereinafter collectively referred to as "progeny line") refers to a lettuce plant of a deposited line or a plant obtained from its progeny line. In the present invention, the progeny line may be a plant obtained by crossing the deposited line with another lettuce plant, or a hybrid of the deposited line with a wild lettuce plant. The progeny line may be obtained, obtainable, or derived directly or indirectly by selfing and / or cross-pollinating the deposited line or its progeny line, or may be derived from a parent line obtained from the deposited line using traditional breeding methods such as selfing and / or cross-pollination. Examples of the progeny line include a selfed progeny line and a first-generation hybrid F1 (first-generation hybrid line, F1 hybrid). In obtaining the progeny line, the deposited line may be used as a female parent, a male parent, or both parents.

[0042] In the present invention, "crossing" refers to the crossing of two parent lines. The crossing may be "cross-pollination" or "self-pollination." Cross-pollination refers to fertilization by the union of two gametes originating from different plants. Self-pollination refers to the transfer of pollen from anthers to the stigma of the same plant. Self-pollination can also be referred to as, for example, selfing. The crossing may include backcrossing, which is one of the traditional breeding methods.

[0043] The "backcrossing" technique is a traditional breeding technique in which breeders repeatedly cross a progeny line of a hybrid with one of the parent lines to introduce a trait into a plant or variety. The plant containing the trait to be introduced can be referred to as, for example, a donor plant. The plant into which the trait is introduced can be referred to as, for example, a recurrent parent. Backcrossing can be performed by crossing a donor plant with the recurrent parent. A first-generation hybrid F1 (first-generation hybrid line, F1 hybrid) can be obtained by the first cross. The progeny line possessing the trait is then crossed with the recurrent parent. The trait of the donor plant can then be introduced into the recurrent parent by backcrossing and / or selfing for several generations.

[0044] In the present invention, the progeny line may be regenerated from a cell culture or tissue culture, protoplast or part of a plant derived from the deposited line, or may be obtained by self-pollination of the deposited line, or may be obtained by producing seeds from a plant of the deposited line.

[0045] In the present invention, the term "regeneration" refers to the generation or vegetative propagation of plants from cell culture, tissue culture or protoplasts.

[0046] The "tissue culture" or "cell culture" may be a composition containing isolated cells of the same or different types, or may be a collection of cells organized into plant parts. Methods for tissue culture of various tissues of lettuce plants and for regenerating plants from such tissue cultures are well known, see, for example, References 1 to 3 below. Reference 1: Pink DA and Carter PJ, “Propagation of lettuce (Lactuca sativa) breeding material by tissue culture”, Ann. Appl. Biol., 1987, vol. 110, pages 611-616 Reference 2: Ampomah-Dwamena C., Conner AJ and Fautrier AG, “Genotypic response of lettuce cotyledons to regeneration in vitro”, Sci. Hort., 1997, vol. 71, pages 137-145 Reference 3: Takayuki Mizutani, Takayuki Tanaka, "Nodal culture of flower stalks of lettuce and its wild relative Lactuca serriola", Horticultural Research, 2008, Vol. 7, No. 1, pp. 17-21

[0047] The progeny line may have desired traits. For example, when grown under the same cultivation conditions, the progeny line may have "essentially all physiological and morphological characteristics of the deposited line." Specifically, the progeny line may have traits in common with the corresponding deposited line (the derived deposited line). Specific examples of the progeny line include one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, eleven or more, twelve or more, thirteen or more, or fourteen or more traits that are identical to those of the deposited line. The progeny line may be a plant that possesses the major traits of the deposited line. The major traits include traits 40 and 59 in Table 1, and resistance to the root rot fungus race Yuma, i.e., traits (1), (2), and (14) above. Furthermore, the major traits include traits with trait numbers 1, 4, 5, 8, 9, 13, 22, 24, 26, 30, and 31 in Table 1, i.e., the traits (3) to (13) above. The progeny line may be a plant that has the same traits as the deposited line except for, for example, 14 or less, 13 or less, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait, i.e., it may be a plant that differs from the deposited line in 14 or less, 13 or less, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait. The "traits different from those of the deposited line" may be major traits of the deposited line or traits other than the major traits of the deposited line, but traits other than the major traits of the deposited line are preferred. The "traits different from those of the deposited line" can be achieved, for example, by introducing traits and / or genes described below. In the progeny line, all traits such as trait numbers 40, 59, 1, 4, 5, 8, 9, 13, 22, 24, 26, 30, and 31, as well as resistance to the root rot fungus race Yuma, may be the same as those of the deposited line. Examples of traits different from those of the deposited line include resistance to downy mildew (Bl16-37, Bl5-11US), bacterial rot resistance, corky root resistance, lettuce mosaic virus resistance, aphid resistance, leafminer resistance, and resistance to browning of cut surfaces.Each trait can be introduced, for example, by crossing with a known plant that has a genetic locus associated with each trait.

[0048] The progeny line obtained by the "selfing" or the lettuce plants obtained from the deposited line or its progeny line by the "regeneration" have "essentially all of the physiological and morphological characteristics of the deposited line" when grown under the same cultivation conditions.

[0049] The progeny line may include cells containing at least one set of chromosomes derived from the deposited line. The progeny line may, for example, have at least about 10%, 20%, 30%, 40%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of its alleles derived from the deposited line. That is, the progeny line may have at least about 10%, 20%, 30%, 40%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% genetic complement with the deposited line.

[0050] An "allele" is any one or more genes, all of which are associated with a characteristic or trait in a lettuce plant. In a diploid cell or organism, the pair of alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes.

[0051] The genetic complementarity can be calculated, for example, by decoding molecular markers or nucleotide sequences, comparing them with the molecular markers or nucleotide sequences of TEXLE213131, and calculating the percentage of identity. Examples of molecular markers include single nucleotide polymorphism (SNP) markers, amplified fragment length polymorphism (AFLP) markers, restriction fragment length polymorphism (RFLP) markers, microsatellite markers, sequence-characterized amplified region (SCAR) markers, and cleaved amplified polymorphic sequence (CAPS) markers. Genome analysis methods using molecular markers are well known and widely published (e.g., References 4 and 5 below). The nucleotide sequence can be determined, for example, by extracting DNA from the progeny line and sequencing the DNA. The proportion of alleles derived from the deposited line and the proportion of genetic complementarity can be estimated, for example, by the number of crosses. In this case, the proportion can be estimated from the number of crosses from the deposited line. As a specific example, when the number of crosses from the deposited line is n, the ratio is, for example, {1-(1 / 2) n}×100%. Reference 4: Sinchan Adhikari et.al, “Application of molecular markers in plant genome analysis: a review”, The Nucleus, 2017, Volume 60, Issue 3, pp. 283-297 Reference 5: Elcio P. Guimaraes et.al., “MARKER-ASSISTED SELECTION Current status and future perspectives in crops, livestock, forestry and fish”, 2007, Springer, 29-49

[0052] The proportion of alleles and genetic complementarity derived from the deposited line is preferably, for example, the average of the proportions of multiple progeny lines, where the number is, for example, the number of individuals that can be statistically analyzed, specifically, 200 or more individuals, preferably 200 to 1000 individuals.

[0053] The progeny line may have polymorphisms in the molecular markers derived from the deposited line. The deposited line may, for example, have at least about 10%, 20%, 30%, 40%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of its molecular markers derived from the deposited line. That is, the progeny line may be identical to the deposited line in at least about 10%, 20%, 30%, 40%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of its molecular markers. In the present invention, when, for example, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% or more of the molecular markers of a target lettuce plant match those of the deposited line, the target lettuce plant can be determined (identified, presumed, identified, or differentiated) to be a progeny line of the deposited line.

[0054] The progeny line and the lettuce line TEXLE213131 may have, for example, a mutation or a transgene. In this case, the progeny line has, for example, one, two, three, or four or more traits or transgenes introduced therein, resulting in a modified trait. The progeny line can be produced, for example, by introducing a mutation or a transgene into the deposited line or its progeny line. The mutation may be artificially or naturally introduced; artificial introduction of a mutation can also be referred to as "induction of a mutation." The mutation may be, for example, a chemically induced mutation or a radiation-induced mutation. The mutation may be introduced, for example, by molecular biological techniques or genome editing techniques (see, for example, Reference 6 below). The mutation or transgene may also be introduced into lettuce seeds, lettuce plants, cell cultures, and / or tissue cultures of the deposited line. The transgene may be introduced, for example, by a method using Agrobacterium tumefaciens. Reference 6: Yanfei Mao et.al., “Gene editing in plants: progress and challenges”, National Science Review, 2019, vol. 6, pp. 421-437

[0055] Examples of the one or more traits include resistance to downy mildew (Bl16 to 37, Bl5 to 11US), resistance to bacterial rot, resistance to corky root, resistance to lettuce mosaic virus, resistance to aphids, resistance to leafminers, and resistance to browning of cut surfaces.

[0056] The term "transgene" refers to a desired gene introduced into the genome of a plant, for example, by genetic engineering techniques or traditional breeding methods. The transgene may include, for example, a gene introduced by single-locus conversion. The transgene may be derived from the same species or a different species. The transgene may also be a gene containing the same or a different nucleotide sequence as that of the species from which it originates. In the latter case, the different nucleotide sequence can be prepared, for example, by codon optimization, addition of a transcriptional regulator such as a promoter, or the like, to the same nucleotide sequence. The transgene may include a translated region and an untranslated region.

[0057] <Haploid and doubled haploid plants> Lettuce plants of the present invention may be haploid and / or doubled haploid plants obtained, obtainable, or derived from the deposited line. Haploid and / or doubled haploid plants of the deposited line may be used in methods to produce parent lines of the progeny line. In one embodiment, the present invention may provide haploid and / or doubled haploid plants, plant parts of haploid and / or doubled haploid plants, or seeds of haploid and / or doubled haploid plants.

[0058] The doubled haploid plants can be produced by doubling the chromosomes in haploid plants or cells (see, for example, Reference 7 below). Specifically, haploid pollen or ovules are cultured under specific conditions to form plantlets with 1n chromosomes. The plantlets are then treated with a chemical substance such as colchicine to double the chromosomes. As a result, the cells of the plantlets have 2n chromosomes (doubled haploid). The doubled haploid plants and their progeny can be obtained by growing the plantlets after the treatment. Reference 7: Jim M. Dunwell, “Haploids in flowering plants: origins and exploitation”, Plant Biotechnology Journal, 2010, volume 8, pp. 377-424

[0059] <How to produce lettuce plants> As described above, the method for producing lettuce plants of the present invention includes a hybridization step of hybridizing a first lettuce plant with a second lettuce plant, the first lettuce plant being the lettuce plant of the present invention. The method for producing lettuce plants of the present invention is characterized in that the lettuce plant of the present invention is used as at least one of the parents in the hybridization step, and other steps and conditions are not particularly limited.

[0060] Furthermore, the method for producing lettuce plants of the present invention includes a selfing step of self-pollinating the lettuce plants of the present invention. The production method of the present invention is characterized by self-pollinating the lettuce plants of the present invention, and other steps and conditions are not particularly limited.

[0061] According to the production method of the present invention, progeny lines of the deposited line can be produced. The explanation of the lettuce plant of the present invention can be applied to the production method of the present invention.

[0062] In the present invention, the cross between the first lettuce plant (first parent line) and the second lettuce plant (second parent line) may be, for example, a cross between the same individuals (normal self-pollination), a cross between individuals of the same clone or between individuals of a line maintained as an inbred line (quasi-self-pollination), or a cross between different individuals (cross-pollination). In the case of normal self-pollination, for example, one of the first parent line and the second parent line is the female organ of the same individual, and the other is the pollen of the same individual.

[0063] The seeds produced by crossing (cross-pollination) between said different individuals and the plants obtained by growing the seeds comprise first generation hybrid F1 (hybrid first generation line, F1 hybrid).

[0064] In the present invention, the first parent line is the lettuce plant of the present invention, for example, the lettuce plant deposited under the aforementioned accession number FERM BP-22497 or a progeny line thereof.

[0065] The second parent line is not particularly limited and any lettuce plant can be used. The second parent line may be, for example, a lettuce plant of the same taxonomic species as the first parent line, or a lettuce plant of a different species. The second parent line may be, for example, the deposited line or a progeny line, or another lettuce plant.

[0066] The production method of the present invention may further include, after the crossbreeding step, a growing step of cultivating the progeny line obtained in the crossbreeding step. The growing conditions in the growing step may be, for example, general growing conditions for lettuce plants.

[0067] The lettuce plant of the present invention can be obtained, for example, by the production method of the present invention.

[0068] <How to produce lettuce seeds> The present invention provides a method for producing lettuce seeds. The method for producing lettuce seeds of the present invention includes the steps of selfing or crossing a lettuce plant of the deposited line with another lettuce plant, and optionally producing (collecting or harvesting) the resulting seeds. The seed production method of the present invention may also provide a plant, plant part, or seed by growing seeds of the lettuce plant.

[0069] The seed production method of the present invention may be a method for producing seeds derived from the deposited line. In this case, the seed production method of the present invention may include the step of (a) crossing a plant of the deposited line with another lettuce plant to produce seeds. The seed production method of the present invention may further include the steps of (b) cultivating a lettuce plant from the seed of step (a) to produce a lettuce plant derived from the deposited line, and (c) self-pollinating or cross-breeding the lettuce plant of step (b) with another lettuce plant to produce an additional lettuce plant derived from the deposited line. Furthermore, the seed production method of the present invention may include (d) optionally repeating steps (b) and (c) one or more times to produce an additional lettuce plant derived from the deposited line. In this case, the additional lettuce plant obtained in step (c) can be used as the lettuce plant cultivated in step (b) from the seed of step (a). The "one or more times" may be, for example, 1 to 10 times, 3 to 7 times, or 3 to 5 times. The seed production method of the present invention may further include a step of collecting or harvesting the seeds. The seed production method of the present invention may provide seeds produced by the above method, and plants or plant parts obtained by growing the seeds.

[0070] <Method for producing hybrid lettuce plants> The present invention provides methods for producing hybrid (hybrid) lettuce plants. The methods for producing hybrid plants of the present invention include crossing a lettuce plant of the present invention with another lettuce plant. The methods for producing hybrid plants of the present invention may also include collecting or harvesting seeds obtained by crossing. The methods for producing hybrid plants of the present invention may also provide seeds and hybrid plants or parts of hybrid plant individuals produced by the above methods.

[0071] <Method of introducing new traits> The present invention provides a method for introducing at least one new characteristic or trait (hereinafter collectively referred to as "trait") into the deposited line. The method for introducing a trait of the present invention can also be referred to as, for example, a method for producing a lettuce plant into which a new trait has been introduced. The method for introducing a trait of the present invention includes, for example, (a) crossing a plant of the deposited line with a lettuce plant containing at least one new trait to produce a progeny line, and (b) selecting a progeny line containing at least one new trait. The method for introducing a trait of the present invention includes, for example, (c) crossing the progeny line with the deposited line to produce backcross progeny seeds, and (d) selecting a backcross progeny containing at least one new trait and essentially all of the physiological and morphological characteristics of the deposited line. In steps (b) and (d), the selection of a progeny line having the new trait may be carried out, for example, by detecting the trait, or by detecting a gene or molecular marker associated (linked) with the trait. Examples of the new trait include resistance to downy mildew (Bl16 to 37, Bl5 to 11US), resistance to bacterial rot, resistance to corky root, resistance to lettuce mosaic virus, resistance to aphids, resistance to leafminers, resistance to browning of cut surfaces, etc. Introduction of a new trait by the above method may also be defined as single-locus conversion.

[0072] In the trait introduction method of the present invention, (e) steps (c) and (d) may be optionally repeated one or more times to produce a lettuce plant containing at least one new trait. The lettuce plant containing the new trait may be defined as a lettuce plant containing at least one single-locus conversion. In this case, the progeny line in step (c) of the trait introduction method of the present invention may use the backcross progeny selected in step (d). The lettuce plant obtained or obtainable in step (e) may have essentially all of the physiological and morphological characteristics of the deposited line. The "essentially all physiological and morphological characteristics" can be used in the description of the progeny line by replacing "progeny line" with "lettuce plant obtained or obtainable in step (e)." The "one or more times" may be, for example, 1 to 10 times, 3 to 7 times, or 3 to 5 times. The trait introduction method of the present invention may also include a step of collecting or harvesting seeds. The method for introducing a trait of the present invention may provide seeds produced by the above method, and plants or parts of individual plants obtained by growing the seeds.

[0073] <Method for introducing transgene> The present invention provides a method for producing a plant derived from a deposited line that contains at least one new characteristic or trait. The method for introducing a transgene of the present invention can also be said to be a method for producing a lettuce plant into which a new trait has been introduced.

[0074] The method for introducing a transgene of the present invention includes, for example, introducing a mutation or transgene that confers at least one new trait into a plant of a deposited line. The introduction of the mutation or transgene can be carried out, for example, in the same manner as the introduction of a mutation or transgene into the progeny line. The lettuce plant obtained or obtainable by the introduction step may have essentially all of the physiological and morphological characteristics of the deposited line. The "essentially all physiological and morphological characteristics" can be used in the description of the progeny line by replacing "progeny line" with "lettuce plant obtained or obtainable by the introduction step." The method for introducing a transgene of the present invention may also include a step of collecting or harvesting seeds. The method for introducing a transgene of the present invention may provide seeds produced by the above method, and plants or plant parts obtained by growing the seeds. Examples of the new traits include resistance to downy mildew (Bl16 to 37, Bl5 to 11US), resistance to bacterial rot, resistance to corky root, resistance to lettuce mosaic virus, resistance to aphids, resistance to leafminers, and resistance to browning of cut surfaces.

[0075] <Lettuce plant regeneration and regeneration method> The present invention provides lettuce plants (hereinafter referred to as "regenerants") regenerated from cell cultures, tissue cultures, or protoplasts of the deposited line. The present invention may also provide cell or tissue cultures of regenerable cells or protoplasts derived from the deposited line of lettuce plants. The cells, tissues, or protoplasts may be derived from tissues including leaves, pollen, embryos, cotyledons, hypocotyls, meristematic cells, roots, root tips, anthers, flowers, seeds, or stems.

[0076] The present invention provides a method for propagating or multiplying a lettuce plant of a deposited line. The propagation of the lettuce plant of the deposited line may be vegetative propagation of the lettuce plant of the deposited line. In this case, the method for regenerating a lettuce plant of the present invention includes, for example, (a) collecting tissue capable of propagation from a plant of the deposited line; (b) culturing the tissue to obtain a propagated shoot; and (c) rooting the propagated shoot to obtain a rooted plantlet. The method for regenerating a lettuce plant of the present invention may further include, optionally, (d) growing a plant from the rooted plantlet. For details of the vegetative propagation method, see, for example, References 8 and 9 below. The regeneration method of the present invention may provide, for example, a plantlet, plant, or part of an individual plant regenerated (produced) by the above method. The plant may have essentially all of the physiological and morphological characteristics of the deposited line. The above-mentioned "essentially all physiological and morphological characteristics" can be used in the description of the progeny line by replacing "progeny line" with "regenerated plant." Reference 8: Habtamu Gudisa Megersa, “Propagation Methods of Selected Horticultural Crops by Specialized Organs: Review”, Journal of Horticulture, 2017, Volume 4, Issue 2, 1000198 Reference 9: Nitish Kumar et.al., “In vitro Plant Propagation: A Review”, Journal of Forest Science, 2011, Vol. 21, No. 2, pp. 61-72

[0077] <Harvested and processed products of lettuce plants> The present invention provides harvested and / or processed products of the deposited line or progeny line, wherein the harvested product is a whole plant or a part of an individual plant, preferably including leaves (e.g., head leaves and expanded leaves), leaves and roots, or seeds.

[0078] The processed product includes any product obtained by processing the deposited line or progeny line. The processing is not particularly limited and includes, for example, cutting, slicing, grinding, pureeing, drying, canning, bottling, washing, packaging, freezing, and / or heat treatment. For the deposited line or progeny line, the plant or plant part used for the processed product is, for example, a leaf. The processed product may be, for example, the deposited line or progeny line that has been washed and packaged. The processed product may be, for example, housed in a container of any size or shape. Specific examples of the container include a bag, a box, a carton, and the like.

[0079] <Method for determining genotype> The present invention provides a method for determining or detecting the genotype of a deposited line or a progeny line. The genotype determination method of the present invention includes, for example, (a) obtaining a nucleic acid sample from the deposited line or a progeny line, and (b) detecting a genome in the nucleic acid sample. In step (a), nucleic acid preparation from the deposited line or a progeny line can be performed using a general nucleic acid preparation method for preparing nucleic acid from tissue. In step (b), for example, polymorphisms and / or alleles in the genome in the nucleic acid sample are detected. The polymorphisms and / or alleles can be detected using, for example, single nucleotide polymorphism (SNP) genotyping, amplified fragment length polymorphism detection (AFLP), restriction fragment length polymorphism discrimination (RFLP) of genomic DNA, sequence-characterized amplified region detection (SCAR) of genomic DNA, cleaved amplified polymorphic sequence detection (CAPS) of genomic DNA, random amplified polymorphic detection (RAPD) of genomic DNA, polymerase chain reaction (PCR), DNA sequencing, allele-specific oligonucleotide (ASO) probes, or DNA microarrays. The polymorphisms and / or alleles may be detected, for example, by sequencing the nucleotide sequence of the genome. In step (b), one polymorphism and / or allele in the genomic DNA may be detected, or two or more polymorphisms and / or alleles may be detected. The genotyping method of the present invention may also include a step of storing the detection results of polymorphisms and / or alleles in a computer-readable medium. The invention may also provide a computer-readable medium produced by such a method.

[0080] The genotyping method of the present invention may be performed on any lettuce plant (target lettuce plant) instead of the deposited line or the progeny line. In this case, the genotyping method of the present invention may further include, for example, a step of determining whether the target lettuce plant is the progeny line based on the results of step (b). This determination can also be referred to as discrimination, estimation, appraisal, or identification. The determination can be made, for example, based on the degree of agreement between the results of step (b) and the genotype of the deposited line. [Example]

[0081] The present invention will be described in detail below using examples, but the present invention is not limited to the embodiments described in the examples.

[0082] (1) Breeding of the deposited strain A lettuce breeding line with late bolting and resistance to root rot race 1 was crossed with a lettuce breeding line with excellent head enlargement and early maturity to obtain F1 generation seeds. The F1 generation was cultivated in 2017, and self-fertilized seeds were obtained. In 2018, 640 F2 generation plants were cultivated, and 13 individuals with strong plant vigor, excellent head enlargement, late bolting, and resistance to root rot race 1 and race Yuma were selected and harvested for seed. In 2019, the F3 generation was cultivated, and 22 individuals with strong plant vigor, excellent head enlargement, late bolting, and resistance to root rot race 1 and race Yuma were selected and harvested for seed. Selection and seed harvesting were repeated until the F7 generation was reached in 2023, when it was determined that the desired traits had been fixed and breeding was terminated.

[0083] A total of approximately 2,000 F7 generation plants were cultivated in a field in Yuma, Arizona, USA in 2023 using two cropping methods with September sowing, and it was confirmed that there was no variation in characteristics within the lines, and that the bred lettuce varieties were uniform and stable. Seeds from several lines of the F7 generation were then mixed and designated TEXLE213131, and a portion of this was deposited under accession number FERM BP-22497.

[0084] (2) Traits of the deposited strain The characteristics and traits of the deposited lines were evaluated in accordance with the Ministry of Agriculture, Forestry and Fisheries' criteria for variety registration. The results are shown in Table 2.

[0085] [Table 2]

[0086] The deposited line of lettuce plants has the characteristics and traits shown in Table 2 above, plus resistance to root rot fungus race Yuma.

[0087] Although the present invention has been described above with reference to the embodiments and examples, the present invention is not limited to the above-described embodiments and examples. Various modifications that can be understood by those skilled in the art can be made to the configuration and details of the present invention within the scope of the present invention. [Industrial Applicability]

[0088] As described above, the present invention provides a novel lettuce plant, which is extremely useful in the agricultural field, for example, in breeding. <Additional Notes> Some or all of the above-described embodiments and examples can be described as, but are not limited to, the following supplementary notes. (Appendix 1) A lettuce plant, including the lettuce plant identified by accession number FERM BP-22497 or its progeny. (Appendix 2) The progeny line is a lettuce plant containing 50% or more alleles of the lettuce plant identified by accession number FERM BP-22497. (Appendix 3) The progeny line contains 50% or more alleles of the lettuce plant identified by accession number FERM BP-22497; The progeny line is a lettuce plant having the following traits (1), (2), and (14): (1) The start of bolting is "slightly late"; (2) resistance to root rot race 1 is "highly resistant"; (14) Resistance to the root rot fungus race Yuma is present. (Appendix 4) The progeny line contains 50% or more alleles of the lettuce plant identified by accession number FERM BP-22497; The progeny line is a lettuce plant having the following traits (1) to (14): (1) The start of bolting is "slightly late"; (2) resistance to root rot race 1 is "highly resistant"; (3) The seed color is "white"; (4) The width of the stock is "medium"; (5) The overlap of the upper leaves of the plant is "strong"; (6) The number of leaf lobes is "none or very small"; (7) The leaf shape is "narrow oblate"; (8) Anthocyanin coloration of the leaves is "absent or very weak"; (9) The leaf irregularities are "slightly strong"; (10) The leaf margins are medium in undulation; (11) The depth of the incision on the margin of the leaf is medium; (12) The size of the ball is "medium"; (13) The leaf cross section is "oblate"; (14) Resistance to the root rot fungus race Yuma is present. (Appendix 5) 5. The lettuce plant according to any one of appendices 1 to 4, wherein the lettuce plant is a plant or a part thereof. (Appendix 6) 6. The lettuce plant of any one of appendices 1 to 5, wherein the lettuce plant is a seed. (Appendix 7) A method for producing a lettuce plant, comprising a hybridization step of hybridizing a first lettuce plant with a second lettuce plant, wherein the first lettuce plant is a lettuce plant described in any one of Appendices 1 to 6. (Appendix 8) Seeds are seeds of lettuce variety TEXLE213131, a representative sample of which is a seed of the lettuce plant deposited under accession number FERM BP-22497. (Appendix 9) A lettuce plant of the lettuce variety TEXLE213131, a representative sample being a seed of the lettuce plant deposited under accession number FERM BP-22497. (Appendix 10) 10. A lettuce plant or part thereof, wherein the lettuce plant or part thereof has essentially all of the physiological and morphological characteristics of the lettuce plant of claim 9. (Appendix 11) A progeny lettuce plant of the lettuce plant according to Supplementary Note 9, wherein the progeny lettuce plant contains at least 50% of the alleles of the lettuce plant according to Supplementary Note 9, and the progeny lettuce plant has the following traits (1) to (14): (1) The start of bolting is "slightly late"; (2) resistance to root rot race 1 is "highly resistant"; (3) The seed color is "white"; (4) The width of the stock is "medium"; (5) The overlap of the upper leaves of the plant is "strong"; (6) The number of leaf lobes is "none or very small"; (7) The leaf shape is "narrow oblate"; (8) Anthocyanin coloration of the leaves is "absent or very weak"; (9) The leaf irregularities are "slightly strong"; (10) The leaf margins are medium in undulation; (11) The depth of the incision on the margin of the leaf is medium; (12) The size of the ball is "medium"; (13) The leaf cross section is "oblate"; (14) Resistance to the root rot fungus race Yuma is present. (Appendix 12) 12. A seed that produces the lettuce plant of claim 11. (Appendix 13) Parts of a lettuce plant as described in Appendix 9. (Appendix 14) 14. The plant part of claim 13, wherein the plant part comprises a microspore, pollen, ovary, ovule, embryo sac, egg cell, cutting, root, stem, leaf, cell, or protoplast. (Appendix 15) 10. A method for producing lettuce seeds, the method comprising selfing or crossbreeding the lettuce plant of claim 9 with another lettuce plant, and harvesting the resulting seeds. (Appendix 16) 16. A lettuce seed derived from a lettuce plant produced by the method of claim 15. (Appendix 17) 17. A lettuce plant or part thereof produced by growing the lettuce seed of claim 16. (Appendix 18) 18. The lettuce plant or part thereof according to Supplementary Note 17, wherein the lettuce plant or part thereof contains at least 50% of the alleles of lettuce variety TEXLE213131, a representative sample of which is seed of the lettuce plant deposited under Accession No. FERM BP-22497, and the lettuce plant or part thereof has the following traits (1) to (14): (1) The start of bolting is "slightly late"; (2) resistance to root rot race 1 is "highly resistant"; (3) The seed color is "white"; (4) The width of the stock is "medium"; (5) The overlap of the upper leaves of the plant is "strong"; (6) The number of leaf lobes is "none or very small"; (7) The leaf shape is "narrow oblate"; (8) Anthocyanin coloration of the leaves is "absent or very weak"; (9) The leaf irregularities are "slightly strong"; (10) The leaf margins are medium in undulation; (11) The depth of the incision on the margin of the leaf is medium; (12) The size of the ball is "medium"; (13) The leaf cross section is "oblate"; (14) Resistance to the root rot fungus race Yuma is present. (Appendix 19) 18. The lettuce plant or part thereof of Appendix 17, wherein the lettuce plant or part thereof has essentially all of the physiological and morphological characteristics of lettuce cultivar TEXLE213131, a representative sample of which is seed from the lettuce plant deposited under accession number FERM BP-22497. (Appendix 20) 19. The lettuce plant or part thereof of claim 18, wherein the lettuce plant or part thereof has been modified for one or more traits. (Appendix 21) 21. The lettuce plant or part thereof of claim 20, wherein the modification is achieved by mutagenesis. (Appendix 22) 10. A method for producing seeds of a lettuce plant derived from the lettuce plant of claim 9, said method comprising: (a) crossing lettuce variety TEXLE213131, a representative sample of which is seed from the lettuce plant deposited under accession number FERM BP-22497, with another lettuce plant to produce seed; (b) growing lettuce plants from the seeds of step (a) to produce lettuce plants derived from the lettuce variety TEXLE213131; (c) selfing or crossing the lettuce plants of step (b) with another lettuce plant to produce additional lettuce plants derived from lettuce variety TEXLE213131; (d) optionally repeating steps (b) and (c) one or more times to produce additional lettuce plants derived from lettuce variety TEXLE213131, wherein the lettuce plants in step (b) are grown from the additional lettuce plants of step (c). (Appendix 23) Seeds produced by the method according to Supplementary Note 22, wherein the seeds contain at least 50% of the alleles of the lettuce plant according to Supplementary Note 9, and the lettuce plants grown from the seeds have the following traits (1) to (14): (1) The start of bolting is "slightly late"; (2) resistance to root rot race 1 is "highly resistant"; (3) The seed color is "white"; (4) The width of the stock is "medium"; (5) The overlap of the upper leaves of the plant is "strong"; (6) The number of leaf lobes is "none or very small"; (7) The leaf shape is "narrow oblate"; (8) Anthocyanin coloration of the leaves is "absent or very weak"; (9) The leaf irregularities are "slightly strong"; (10) The leaf margins are medium in undulation; (11) The depth of the incision on the margin of the leaf is medium; (12) The size of the ball is "medium"; (13) The leaf cross section is "oblate"; (14) Resistance to the root rot fungus race Yuma is present. (Appendix 24) 24. A lettuce plant produced by growing seeds of the lettuce plant of claim 23. (Appendix 25) 10. A method for introducing at least one new trait into a lettuce plant according to claim 9, The method comprises: (a) crossing lettuce variety TEXLE213131, a representative sample of which is seed of the lettuce plant deposited under accession number FERM BP-22497, with a lettuce plant having at least one new trait to produce progeny; (b) selecting progeny containing at least one new trait; (c) crossing the progeny with lettuce variety TEXLE213131 to produce backcross progeny; (d) selecting a backcross progeny that contains at least one new trait and has essentially all of the physiological and morphological characteristics of lettuce variety TEXLE213131; (e) optionally repeating steps (c) and (d) one or more times to produce a lettuce plant containing at least one new trait and having essentially all of the physiological and morphological characteristics of lettuce variety TEXLE213131, wherein the lettuce plant in step (c) is a selected backcross progeny of step (d). (Appendix 26) 26. A lettuce plant produced by the method of claim 25. (Appendix 27) 1. A method for producing a lettuce plant derived from lettuce variety TEXLE213131 that contains at least one new trait, the method comprising introducing a mutation or transgene that confers the at least one trait into lettuce variety TEXLE213131, a representative sample of which is seed of the lettuce plant deposited under accession number FERM BP-22497. (Appendix 28) 28. A lettuce plant produced by the method of claim 27. (Appendix 29) 10. A method for producing lettuce heads as a food product, the method comprising harvesting the heads or the heads, expanded leaves and stems of a lettuce plant according to claim 9. (Appendix 30) 10. A processed product of the lettuce plant of claim 9, said processed product comprising cut, sliced, ground, pureed, dried, canned, bottled, washed, packaged, frozen, and / or heat treated head leaves. (Appendix 31) 10. A method for determining the genotype of a lettuce plant or a progeny line thereof according to claim 9, comprising: (a) obtaining a nucleic acid sample from a lettuce plant or its progeny as described in Appendix 9; (b) detecting a polymorphism in the nucleic acid sample; A method comprising: (Appendix 32) 10. A regenerable cell or protoplast tissue culture derived from a lettuce plant according to claim 9. (Appendix 33) 33. The cultured tissue of claim 32, wherein the cells or protoplasts are derived from leaves, pollen, embryos, cotyledons, hypocotyls, meristematic cells, roots, root tips, anthers, flowers, seeds, or stems. (Appendix 34) 34. A lettuce plant regenerated from the culture tissue described in Appendix 33. (Appendix 35) 35. The lettuce plant of claim 34, wherein the lettuce plant has essentially all of the physiological and morphological characteristics of lettuce cultivar TEXLE213131, a representative sample of which is seed of the lettuce plant deposited under accession number FERM BP-22497. (Appendix 36) 10. A method for vegetatively propagating lettuce plants according to claim 9, said method comprising: (a) collecting reproductive tissue from lettuce plants of the lettuce variety TEXLE213131, a representative sample of which is seed from the lettuce plant deposited under accession number FERM BP-22497; (b) culturing the tissue and obtaining proliferated shoots; (c) rooting the propagated shoots and obtaining rooted plantlets; (d) optionally growing plants from the rooted plantlets; A method comprising: (Appendix 37) 37. A lettuce plantlet or plant produced by the method of Appendix 36, wherein the lettuce plantlet or plant has essentially all of the physiological and morphological characteristics of lettuce cultivar TEXLE213131, a representative sample of which is seed of the lettuce plant deposited under accession number FERM BP-22497. (Appendix 38) Lettuce plants, including the lettuce plants identified by accession number FERM BP-22497. (Appendix 39) a progeny line of the lettuce plant described in Appendix 38, The progeny line is a lettuce plant having the following traits (1) to (14): (1) The start of bolting is "slightly late"; (2) resistance to root rot race 1 is "highly resistant"; (3) The seed color is "white"; (4) The width of the stock is "medium"; (5) The overlap of the upper leaves of the plant is "strong"; (6) The number of leaf lobes is "none or very small"; (7) The leaf shape is "narrow oblate"; (8) Anthocyanin coloration of the leaves is "absent or very weak"; (9) The leaf irregularities are "slightly strong"; (10) The leaf margins are medium in undulation; (11) The depth of the incision on the margin of the leaf is medium; (12) The size of the ball is "medium"; (13) The leaf cross section is "oblate"; (14) Resistance to the root rot fungus race Yuma is present. (Appendix 40) 39. A lettuce plant comprising a first generation hybrid line of the lettuce plant of claim 38 or 39. (Appendix 41) 41. The lettuce plant of any one of appendices 38 to 40, wherein the lettuce plant is a plant or part thereof. (Appendix 42) 42. The lettuce plant of any one of claims 38 to 41, wherein the lettuce plant is a seed. (Appendix 43) A method for producing a lettuce plant, comprising a self-pollinating step of self-pollinating the lettuce plant according to any one of appendices 38 to 42. (Appendix 44) A method for producing a lettuce plant, comprising a hybridization step of hybridizing the lettuce plant according to any one of appendices 38 to 42 with another lettuce plant. (Appendix 45) 45. A method for producing a lettuce plant according to claim 43 or 44, comprising a seed harvesting step.

Claims

1. 1. Seeds of a lettuce plant, said lettuce being of the lettuce line TEXLE213131, a representative sample of said seeds being deposited under accession number FERM BP-22497.

2. 10. A lettuce plant grown from the seed of claim 1.

3. A lettuce plant having essentially all of the physiological and morphological characteristics of the lettuce plant of claim 2.

4. A tissue culture of the lettuce plant of claim 2.

5. A lettuce plant regenerated from the tissue culture of claim 4.

6. 1. A lettuce plant of the lettuce line TEXLE213131, a representative sample of seeds of said plant being deposited under accession number FERM BP-22497, said plant further comprising a mutation and / or a transgene.

7. 1. A lettuce plant of lettuce line TEXLE213131, a representative sample of seeds of said plant being deposited under accession number FERM BP-22497, said plant further comprising a single locus conversion.

8. A progeny line of the lettuce plant according to claim 2, comprising at least 50% of the alleles of the lettuce line TEXLE213131 and having the following traits (1), (2), and (14): (1) The start of bolting is "slightly late"; (2) Resistance to root rot fungus race 1 is "highly resistant"; (14) Resistance to the root rot fungus race Yuma is "yes"

9. A part of a lettuce plant according to any one of claims 2 to 8.

10. A hybrid first generation lettuce plant, or a part thereof, having the lettuce plant according to any one of claims 2 to 8 as a first parent line and another lettuce plant as a second parent line.

11. A method for producing a lettuce plant, comprising a self-pollinating step of self-pollinating the lettuce plant according to any one of claims 2 to 8.

12. A method for producing lettuce plants, comprising a hybridization step of hybridizing the lettuce plant according to any one of claims 2 to 8 with another lettuce plant.

13. A method for producing lettuce plants, comprising introducing a mutation into the lettuce seeds of claim 1 and growing the seeds.

14. A method for producing a lettuce plant, comprising introducing a mutation into the lettuce plant according to any one of claims 2 to 8.

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