Polymer nanoparticles

Abstract

The objective is to provide a method for producing polymer nanoparticles and polymer nanoparticles that can safely and stably produce spherical polymer nanoparticles made of a predetermined resin species. [Solution] A method for producing polymer nanoparticles comprises: a dissolution step of dissolving a thermoplastic resin in a first organic solvent to form a polymer solution; an addition step of adding the polymer solution to a dispersion solvent containing a second organic solvent that is a good solvent for the first organic solvent and a poor solvent or non-solvent for the thermoplastic resin, thereby precipitating polymer nanoparticles in the dispersion solvent; and a vaporization step of vaporizing the dispersion solvent to separate the polymer nanoparticles from the dispersion solvent.

Description

Technical Field

[0001] The present invention relates to polymer nanoparticles.

Background Art

[0002] Plastics have become an indispensable material in modern life because they are durable and lightweight. However, due to the improper treatment of used plastics, plastics on the micrometer order or plastics on the nanometer order flow into the environment, causing large-scale environmental pollution, which has become a problem. In particular, the problem of plastics in the ocean has been addressed as an important international issue, and the need for research on nanoplastics as a major risk factor has been recognized worldwide.

[0003] In order to evaluate the environmental risks of nanoplastics, it is necessary to use particles made of a standard substance having the properties of general plastics (hereinafter also referred to as standard substance particles), and spherical nanoparticles made of a polymer containing no additives such as surfactants are required. However, the lack of such standard substance particles is currently a problem.

[0004] Conventionally, methods for producing polymer particles include mechanical grinding methods, methods by polymerization, methods for dispersing droplets, methods by dispersion in a non-solvent (a solvent in which the solubility of the resin is extremely low), solvent dissolution methods, and the like.

[0005] As methods by polymerization, for example, methods by emulsion polymerization and methods by suspension polymerization are known (Patent Document 1).

[0006] As methods for dispersing droplets, for example, a method of spraying a solvent in which a polymer is dissolved (Patent Document 2) and a method of dispersing a solvent in which a polymer is dissolved by centrifugal force are known (Patent Document 3).

[0007] As a method involving dispersion in a non-solvent, a known method involves dispersing and melting the polymer in a non-solvent such as silicone oil or polyethylene glycol under a heated environment, cooling it, and then washing away the non-solvent (Patent Documents 4, 5).

[0008] Known solvent dissolution methods include: (1) a method in which the polymer is dissolved in a solvent and then particles are precipitated by adding a non-solvent, cooling, etc., or a method in which the polymer is dissolved in a solvent and then particles are obtained by evaporating the solvent (Patent Documents 6, 7, 8); (2) a method in which polymer particles are formed by dropping a solvent containing polyethylene terephthalate (PET) into a dispersion solvent consisting of water at room temperature (Non-Patent Document 1); (3) a method in which spherical particles with a diameter of about 150 mm are precipitated by using a microreactor to mix a tetrahydrofuran solution of polystyrene with water or an aqueous sodium chloride solution (Non-Patent Document 2); and (4) a method in which polymers such as high-density polyethylene are dissolved in tetrahydrofuran at room temperature to saturation solubility, and then polymer particles are precipitated by adding tetrahydrofuran containing water (Non-Patent Document 3). [Prior art documents] [Patent Documents]

[0009] [Patent Document 1] Japanese Patent Application Publication No. 3-131603 [Patent Document 2] Japanese Patent Application Publication No. 59-172521 [Patent Document 3] Japanese Unexamined Patent Publication No. 59-172522 [Patent Document 4] Japanese Patent Application Publication No. 58-219240 [Patent Document 5] Japanese Patent Publication No. 61-271330 [Patent Document 6] Japanese Patent Publication No. 2011-074345 [Patent Document 7] Japanese Patent Application Publication No. 3-128939 [Patent Document 8] International Publication No. 2010 / 140500 [Non-patent literature]

[0010] [Non-Patent Document 1] Johnson et al., Nanoscale Adv., 2021, 3, 339 [Non-Patent Document 2] Zhang et al., Soft Matter, 2012, 8, 86-93 [Non-Patent Document 3] Ganguly et al., ACS Earth and Space Chemistry 2019 3 (9), 1729-1739 [Overview of the project] [Problems that the invention aims to solve]

[0011] However, mechanical grinding methods make it difficult to control the particle shape, and spherical particles cannot be formed. Furthermore, the polymerization method described in Patent Document 1 requires the use of a surfactant as a dispersant, resulting in residual dispersant on the polymer particles. In addition, it has been reported that the polymer particles obtained by the method described in Patent Document 1 have an average size of several μm or more, and their molecular weight decreases through emulsion polymerization or suspension polymerization, making them unsuitable for use as a standard material.

[0012] Furthermore, the droplet dispersion methods described in Patent Documents 2 and 3 require large-scale equipment and complex processes, and cannot be considered efficient in terms of production considering the demand for standard materials. In addition, these methods produce very large average particle sizes ranging from a few μm to approximately 200 μm, making it impossible to produce nanoparticles.

[0013] Furthermore, the dispersion method in non-solvents described in Patent Documents 4 and 5 uses silicone oil or polyethylene glycol as the solvent. However, these solvents are non-volatile and highly viscous, making it highly likely that they will remain as impurities in the particles. Moreover, such methods can only produce particles with an average particle size of several micrometers or more, and cannot be used to produce nanoparticles.

[0014] In addition, in the solvent dissolution methods of Patent Documents 6 to 8, only particles with an average size of about several tens of μm can be obtained, and variations occur in the size of the obtained particles. Regarding the solvent dissolution method, when actually producing particles by the method disclosed in Non-Patent Document 1, the dispersibility in water of the solution containing PET dropped into water is poor, spherical particles cannot be obtained, and numerous PET particles aggregate, making it impossible to stably produce particles with a small particle size. Tetrahydrofuran used in the solvent dissolution methods of Non-Patent Documents 2 and 3 has a risk of generating explosive peroxides by oxidation, and it is difficult to use industrially. As a means of preventing the oxidation of tetrahydrofuran, a means of adding a stabilizer is known, but since the stabilizer is non-volatile, impurities remain in the particles of the polymer formed by this means. Also, it is difficult to obtain spherical particles by the method disclosed in Non-Patent Document 3.

[0015] The present invention has been made in view of the above circumstances, and an object thereof is to provide a method for producing polymer nanoparticles that can safely and stably produce spherical polymer nanoparticles composed of a predetermined resin type, and polymer nanoparticles.

Means for Solving the Problems

[0016] As a result of intensive research, the present inventors dissolved a thermoplastic resin in a first organic solvent to form a polymer solution, and then added the polymer solution to a dispersion solvent containing a second organic solvent that is a poor solvent or non-solvent for the thermoplastic resin and a good solvent for the first organic solvent. Thereafter, by vaporizing the dispersion solvent, while dissolving the first organic solvent in the polymer solution in the second organic solvent, the thermoplastic resin dissolved in the polymer solution can be precipitated as polymer nanoparticles in the dispersion solvent. Subsequently, since the polymer nanoparticles and the dispersion solvent can be separated, it has been found that spherical polymer nanoparticles composed of a thermoplastic resin containing no impurities such as organic solvents and surfactants can be obtained safely and stably.

[0017] That is, the present invention provides the following configurations. (1) A dissolving step of dissolving a thermoplastic resin in a first organic solvent to form a polymer solution; An adding step of adding the polymer solution to a dispersion solvent containing a second organic solvent that is a good solvent for the first organic solvent and a poor solvent or a non-solvent for the thermoplastic resin, and precipitating polymer nanoparticles in the dispersion solvent; and a vaporizing step of vaporizing the dispersion solvent to separate the polymer nanoparticles and the dispersion solvent. A method for producing polymer nanoparticles.

[0018] (2) The method for producing polymer nanoparticles according to (1) above, wherein the content of the thermoplastic resin with respect to the total mass of the polymer solution is 0.01% by mass or more and 5% by mass or less.

[0019] (3) The method for producing polymer nanoparticles according to (1) or (2) above, wherein the thermoplastic resin is any one selected from polyolefin resins, polystyrene resins, polyvinyl chloride resins, and polyester resins.

[0020] (4) The method for producing polymer nanoparticles according to any one of (1) to (3) above, wherein the first organic solvent is any one selected from xylene, toluene, cyclohexanone, and hexafluoroisopropanol.

[0021] (5) The method for producing polymer nanoparticles according to any one of (1) to (4) above, wherein in the adding step, the temperature of the dispersion solvent when precipitating polymer nanoparticles in the dispersion solvent is 10°C or higher and 150°C or lower.

[0022] (6) The method for producing polymer nanoparticles according to any one of (1) to (4) above, wherein in the adding step, the temperature of the dispersion solvent when precipitating polymer nanoparticles in the dispersion solvent is 10°C or higher and 100°C or lower lower than the lower temperature of the boiling points of the first organic solvent and the dispersion solvent.

[0023] (7) A method for producing polymer nanoparticles according to any one of (1) to (6) above, wherein the dispersion solvent consists of the second organic solvent, and the second organic solvent is dimethyl sulfoxide.

[0024] (8) A method for producing polymer nanoparticles according to any one of (1) to (6) above, wherein the dispersion solvent consists of water and the second organic solvent, the content of water relative to the total mass of the dispersion solvent is 1% by mass or more and 50% by mass or less, and the second organic solvent is selected from dimethyl sulfoxide, ethanol, and acetone.

[0025] (9) A method for producing polymer nanoparticles according to any one of (1) to (8) above, wherein in the addition step, the polymer solution is gradually added to the dispersion solvent while stirring the dispersion solvent.

[0026] (10) A method for producing polymer nanoparticles according to any one of (1) to (8) above, wherein the rate of adding the polymer solution in the addition step is 1 to 40 (mL / min).

[0027] (11) Polymer nanoparticles made of a thermoplastic resin with a weight-average molecular weight of 30,000 or more and 700,000 or less, which are spherical and have an average particle size of less than 1 μm.

[0028] (12) The polymer nanoparticles described in (11) above, wherein the thermoplastic resin is selected from polyolefin resins, polystyrene resins, polyvinyl chloride resins, and polyester resins.

[0029] (13) Polymer nanoparticles as described in (11) or (12) above, which are used as standard materials for measurement or analysis. [Effects of the Invention]

[0030] According to the present invention, a method for producing polymer nanoparticles that can safely and stably produce spherical polymer nanoparticles made of a predetermined resin species, and polymer nanoparticles themselves can be provided. [Brief explanation of the drawing]

[0031] [Figure 1] This is a flowchart of a method for producing polymer nanoparticles according to an embodiment of the present invention. [Figure 2] Figure 1 is a schematic perspective view showing one form of the additive process. [Figure 3] This is a scanning electron microscope image of the polymer nanoparticles prepared in Example 1. [Figure 4] This is a scanning electron microscope image of the polymer nanoparticles prepared in Example 2. [Figure 5] This is a scanning electron microscope image of the polymer nanoparticles prepared in Example 3. [Figure 6] This is a scanning electron microscope image of the polymer nanoparticles prepared in Example 4. [Figure 7] This is a scanning electron microscope image of the polymer nanoparticles prepared in Example 5. [Figure 8] This is a scanning electron microscope image of the polymer nanoparticles prepared in Example 6. [Figure 9] This is a scanning electron microscope image of the polymer nanoparticles produced in Manufacturing Example 1. [Figure 10] This is a scanning electron microscope image of the polymer nanoparticles produced in Manufacturing Example 2. [Figure 11] This is a scanning electron microscope image of the polymer nanoparticles produced in Manufacturing Example 3. [Figure 12] This is a scanning electron microscope image of the polymer nanoparticles produced in Manufacturing Example 4. [Figure 13] This is an image of the filter used in Example 7 to determine the manufacturing efficiency. [Figure 14] This is an image of the filter used to determine the manufacturing efficiency in manufacturing example 5. [Figure 15] This is an image of the filter used to determine the manufacturing efficiency in Comparative Example 1. [Modes for carrying out the invention]

[0032] The following describes an example of an embodiment of the present invention. The present invention is not limited to the following example.

[0033] [Method for producing polymer nanoparticles] Figure 1 is a flowchart showing an example of a method for producing polymer nanoparticles according to an embodiment of the present invention. The method for producing polymer nanoparticles according to this embodiment includes a dissolution step of dissolving a thermoplastic resin in a first organic solvent to form a polymer solution, an addition step of adding the polymer solution to a dispersion solvent containing a second organic solvent that is a good solvent for the first organic solvent and a poor solvent or non-solvent for the thermoplastic resin, thereby precipitating polymer nanoparticles in the dispersion solvent, and a vaporization step of vaporizing the dispersion solvent to separate the polymer nanoparticles from the dispersion solvent. Without departing from the spirit of the present invention, other steps may be included before the dissolution step, after the vaporization step, or between each step.

[0034] <Dissolution process> The dissolution step involves dissolving the thermoplastic resin in a first organic solvent to form a polymer solution. In the dissolution step, for example, an organic solvent that is a good solvent for the thermoplastic resin is used as the first organic solvent. The first organic solvent used in the dissolution step is not particularly limited, but is typically selected from xylene, toluene, cyclohexanone, or hexafluoroisopropanol.

[0035] The thermoplastic resin used in the dissolution process is not particularly limited, but is selected from, for example, polyolefin resins, polystyrene resins, polyvinyl chloride resins, and polyester resins. Preferably, the thermoplastic resin is selected from the group consisting of polyolefin resins, polystyrene resins, and polyvinyl chloride resins.

[0036] Examples of polyolefin resins include high-density polyethylene (HDPE), low-density polyethylene (LDPE), polypropylene (PP), polybutene-1, ethylene-propylene copolymer, ethylene-α-olefin copolymer, and polymer blends thereof.

[0037] Examples of polystyrene-based resins include rubber-reinforced styrene-based resins such as polystyrene (PS), high-impact polystyrene (HIPS), and medium-impact polystyrene, as well as styrene-acrylonitrile copolymer (SAN resin), acrylonitrile-butylacrylate rubber-styrene copolymer (AAS resin), acrylonitrile-ethylenepropyl rubber-styrene copolymer (AES), acrylonitrile-polyethylene chloride-styrene copolymer (ACS), ABS resin (e.g., acrylonitrile-butadiene-styrene copolymer), and polymer blends thereof.

[0038] Examples of polyvinyl chloride-based resins include polyvinyl chloride (PVC), polyvinylidene chloride (PVDC), and polymer blends thereof.

[0039] Examples of polyester resins include polyethylene terephthalate (PET), polytrimethylene terephthalate (PTT), polybutylene terephthalate (PBT), polyethylene naphthalate (PEN), polybutylene naphthalate (PBN), and polymer blends thereof.

[0040] The number-average molecular weight (Mn) of the above thermoplastic resins is, for example, 5,000 to 60,000, preferably 8,000 to 55,000 for polyolefin resins, 70,000 to 130,000, preferably 90,000 to 120,000 for polystyrene resins, 20,000 to 70,000, preferably 30,000 to 50,000 for polyvinyl chloride resins, and 8,000 to 42,000, preferably 8,000 to 30,000 for polyester resins. The weight-average molecular weight (Mw) of the above thermoplastic resins is, for example, 30,000 to 700,000, preferably 70,000 to 300,000 for polyolefin resins, 150,000 to 350,000, preferably 200,000 to 300,000 for polystyrene resins, 30,000 to 180,000, preferably 40,000 to 120,000 for polyvinyl chloride resins, and 30,000 to 80,000, preferably 30,000 to 50,000 for polyester resins. The polydispersity (Mw / Mn) of the above thermoplastic resins is, for example, 1 to 20, preferably 2 to 16. The number-average molecular weight, weight-average molecular weight, and polydispersity of the above thermoplastic resins are measured using an apparatus based on the same principle as HLC-8321GPC / HT (product name of Tosoh Corporation), and the resulting peaks are analyzed.

[0041] In the dissolution process, the content of thermoplastic resin relative to the total mass of the polymer solution (polymer concentration) can be, for example, 0.01% by mass or more and 5% by mass or less, and preferably 0.1% by mass or more and 2% by mass or less. By setting the content of thermoplastic resin relative to the total mass of the polymer solution to such a content in the dissolution process, it is easier to suppress the formation of clumps in the final polymer nanoparticles and to control the particle size of the polymer nanoparticles.

[0042] In the dissolution process, the temperature of the polymer solution can be set to, for example, 100°C to 150°C for polyolefin resins, preferably 120°C to 150°C, and to 10°C to 50°C for polystyrene resins, polyvinyl chloride resins, and polyester resins, preferably 35°C to 50°C. By setting the temperature of the polymer solution to these temperatures in the dissolution process, the thermoplastic resin dissolves sufficiently, and the amount of thermoplastic resin obtained as the finished product can be increased.

[0043] <Addition process> In the addition step, as described above, a solution containing a second organic solvent that is a good solvent for the first organic solvent and a poor solvent or non-solvent for the thermoplastic resin is used as the dispersion solvent. In this embodiment, a good solvent is a solvent that can dissolve the first organic solvent, for example, one that is miscible with the first organic solvent in any ratio under standard conditions. In this embodiment, a poor solvent or non-solvent is a solvent that does not dissolve the thermoplastic resin well or cannot dissolve it, for example, one in which the solubility of the thermoplastic resin under standard conditions is 0.01 or less.

[0044] Figure 2 is a schematic perspective view showing one form of the additive process in Figure 1. In the addition step, for example, as shown in Figure 2, the polymer solution 50 is gradually added to the dispersion solvent 20 in the container 10 using a cylinder 40 while stirring the dispersion solvent 20 with a stirrer 30, thereby precipitating polymer nanoparticles 60. As long as polymer nanoparticles can be precipitated from the polymer solution, other addition methods can be employed, not limited to the configuration shown in Figure 2.

[0045] The dispersion solvent is a solution containing a second organic solvent, preferably containing the second organic solvent as the main component. In this embodiment, "containing as the main component" means that the content of the second organic solvent relative to the total mass of the dispersion solvent is greater than 50% by mass. Furthermore, the second organic solvent contained in the dispersion solvent preferably has a boiling point that can be easily vaporized in the vaporization step by a known method described later, and for example, it is preferable that it has a boiling point of less than 200°C.

[0046] The dispersion solvent may consist of a second organic solvent, or it may consist of water and a second organic solvent. When using a polymer solution containing a polyolefin resin as the thermoplastic resin and a solvent with a higher boiling point than water, such as xylene, as the first organic solvent, it is preferable that the dispersion solvent be a second organic solvent. The polymer solution used here, for example, contains a solvent with a boiling point several tens of degrees higher than water. In this case, it is preferable that the second organic solvent be dimethyl sulfoxide. When the first organic solvent contained in the polymer solution is a solvent with a boiling point several tens of degrees higher than water, such as xylene, using such a dispersion solvent allows for the deposition of polymer nanoparticles with stable shape and particle size, even if the temperature of the dispersion solvent (reaction temperature) is increased when precipitating polymer nanoparticles in the dispersion solvent, the composition of the dispersion solvent does not change, and polymer nanoparticles with stable shape and particle size can be precipitated.

[0047] When using a thermoplastic resin such as a polyolefin resin, which needs to be kept at a temperature of 100°C or higher to maintain its dissolved state in the solvent, the dispersion solvent is preferably a second organic solvent. In this case, the second organic solvent is preferably a solvent with a boiling point of 120°C or higher, such as dimethyl sulfoxide. By using such a dispersion solvent, even if the temperature of the dispersion solvent (reaction temperature) is increased when precipitating polymer nanoparticles in the dispersion solvent, the composition of the dispersion solvent does not change, and polymer nanoparticles with stable shape and particle size can be precipitated.

[0048] When using a thermoplastic resin that dissolves in a first organic solvent at room temperature, such as a polystyrene resin, polyvinyl chloride resin, or polyester resin, the dispersion solvent consists of water and a second organic solvent. The water content relative to the total mass of the dispersion solvent is, for example, 1% by mass or more, preferably 20% by mass or more, more preferably 25% by mass or more, and even more preferably 28% by mass or more. The water content relative to the total mass of the dispersion solvent is, for example, 50% by mass or less, more preferably 45% by mass or less, preferably 40% by mass or less, and even more preferably 35% by mass or less.

[0049] Furthermore, the preferred water content in the dispersion solvent varies depending on the type of thermoplastic resin. When using a polystyrene-based resin as the thermoplastic resin, the water content relative to the total mass of the dispersion solvent is preferably 10% by mass or more, more preferably 20% by mass or more, even more preferably 25% by mass or more, preferably 33% by mass or less, more preferably 32% by mass or less, and even more preferably 30% by mass or less. When using a polystyrene-based resin as the thermoplastic resin, having a water content relative to the total mass of the dispersion solvent above a predetermined value makes it easier to suppress the bonding of polymer nanoparticles. Also, when using a polystyrene-based resin as the thermoplastic resin, having a water content relative to the total mass of the dispersion solvent below a predetermined value suppresses the formation of clumps and makes it easier to obtain high production efficiency.

[0050] When using a polyvinyl chloride resin as the thermoplastic resin, the water content relative to the total mass of the dispersion solvent is preferably 25% by mass or more, preferably 37% by mass or less, more preferably 36% by mass or less, and even more preferably 35% by mass or less. When using a polyvinyl chloride resin as the thermoplastic resin, having a water content relative to the total mass of the dispersion solvent above a predetermined value makes it easier to suppress the bonding of polymer nanoparticles together. Furthermore, when using a polyvinyl chloride resin as the thermoplastic resin, having a water content relative to the total mass of the dispersion solvent below a predetermined value allows for more stable production of spherical polymer nanoparticles.

[0051] When a polyester resin is used as the thermoplastic resin, the water content is preferably 20% by mass or more, more preferably 30% by mass or more, preferably 50% by mass or less, more preferably 45% by mass or less, even more preferably 40% by mass or less, and even more preferably 35% by mass or less. When a polyester resin is used as the thermoplastic resin, the water content relative to the total mass of the dispersion solvent being above a predetermined value suppresses the formation of clumps due to aggregation of polymer nanoparticles and improves the manufacturing efficiency. Furthermore, when a polyester resin is used as the thermoplastic resin, the water content relative to the total mass of the dispersion solvent being below a predetermined value improves the manufacturing efficiency.

[0052] When using thermoplastic resins that dissolve in a first organic solvent at room temperature, such as polystyrene resins, polyvinyl chloride resins, and polyester resins, as described above, the second organic solvent used with water as a dispersion solvent is preferably one of ethanol, dimethyl sulfoxide, or acetone.

[0053] Specific combinations of thermoplastic resins and organic solvents include, for example, when using a polymer solution containing polystyrene as the thermoplastic resin and toluene as the first organic solvent, ethanol can be used as the second organic solvent, and a dispersion solvent consisting of water and ethanol can be used. When using a polymer solution containing polyvinyl chloride as the thermoplastic resin and cyclohexanone as the first organic solvent, dimethyl sulfoxide can be used as the second organic solvent, and a dispersion solvent consisting of water and dimethyl sulfoxide can be used. When using a polymer solution containing polyethylene terephthalate as the thermoplastic resin and hexafluoroisopropanol as the first organic solvent, acetone can be used as the second organic solvent, and a dispersion solvent consisting of water and acetone can be used. By selecting the above-mentioned combination of polymer solution and dispersion solvent, it is possible to precipitate polymer nanoparticles with smaller particle sizes and a higher degree of sphericity.

[0054] In the addition step, it is preferable to maintain the dispersion solvent at a predetermined temperature in order to precipitate polymer nanoparticles in the dispersion solvent. While not particularly limited, it is preferable that the temperature of the dispersion solvent (reaction temperature) when precipitation of polymer nanoparticles in the dispersion solvent during the addition step be 100°C to 150°C for polyolefin resins, and 10°C to 50°C for polystyrene resins, polyvinyl chloride resins, and polyester resins. More preferably, it is 110°C to 140°C for polyolefin resins, 40°C to 50°C for polystyrene resins, 40°C to 43°C for polyvinyl chloride resins, and 40°C to 50°C for polyester resins. By setting the reaction temperature within this range, it is easier to control the shape and particle size of the precipitated polymer nanoparticles.

[0055] The reaction temperature may be adjusted depending on the type of dispersion solvent and the type of first organic solvent. While not particularly limited, it is preferable to set the reaction temperature to be 10°C to 100°C lower than the lower of the boiling points of the first organic solvent and the dispersion solvent, and more preferably 20°C to 100°C lower. Such a reaction temperature makes it easier to control the shape and particle size of the polymer nanoparticles.

[0056] In the addition step, it is preferable to gradually add the polymer solution to the dispersion solvent while stirring the dispersion solvent. The dispersion solvent can be stirred by known methods. The dispersion solvent can be stirred using, for example, a stirring bar such as a stirrer.

[0057] In the addition step, the rate of addition of the polymer solution (dropping rate of polymer droplets) is, for example, 1 to 40 mL / min, preferably 2 mL / min or more, more preferably 3 mL / min or more, and preferably 10 mL / min or less. By gradually adding the polymer solution to the dispersion solvent and / or adding the polymer solution to the dispersion solvent while stirring the dispersion solvent, the polymer solution before precipitation is dispersed, and as a result, the precipitation of polymer nanoparticles as clumps is easily suppressed.

[0058] <Vaporization process> In the vaporization step, the dispersion solvent is vaporized to separate the polymer nanoparticles from the dispersion solvent. The vaporization step can be carried out by known methods such as heating or reduced pressure.

[0059] When the vaporization process is carried out by heating, for example, the dispersion solvent is heated to a temperature above its boiling point using a known heating method. By heating, the dispersion solvent attached to the polymer nanoparticles is vaporized, and the polymer nanoparticles and the dispersion solvent can be separated. Alternatively, the polymer nanoparticles may be extracted from the dispersion solvent by filtration or the like, and the extracted polymer nanoparticles may be heated.

[0060] When the vaporization process is carried out under reduced pressure, for example, the polymer nanoparticles extracted from the dispersion solvent can be transferred to another container such as a petri dish, and the pressure can be reduced to vaporize the dispersion solvent attached to the polymer nanoparticles, thereby separating the polymer nanoparticles from the dispersion solvent. Alternatively, the polymer nanoparticles extracted from the dispersion solvent may be heated under reduced pressure.

[0061] As described above, in the method for producing polymer nanoparticles according to this embodiment, the thermoplastic resin dissolved in the polymer solution is precipitated as polymer nanoparticles in a dispersion solvent containing the second organic solvent while the first organic solvent in the polymer solution is dissolved in the second organic solvent. This production method allows for the safe and stable production of polymer nanoparticles made of a predetermined type of thermoplastic resin without using organic solvents that can form explosive compositions or impurities such as surfactants. For example, polymer nanoparticles made of a predetermined type of thermoplastic resin can be produced without using tetrahydrofuran or the like, while suppressing aggregation and having most of them be spherical in shape.

[0062] The above-mentioned production efficiency (%) is an indicator of whether or not non-aggregated polymer nanoparticles can be stably produced. Production efficiency is determined by filtering the polymer nanoparticles obtained by the above-mentioned polymer nanoparticle production method through a filter to remove coarse particles (aggregates), dividing the weight of the polymer nanoparticles by the weight of the thermoplastic resin added in the addition step, and multiplying by 100. Here, the filters used to determine the production efficiency are, for example, two filters: a 0.25 mm filter and a 2 μm filter. A production efficiency of 93% or higher is preferable, and 95% or higher is more preferable.

[0063] [Composition of polymer nanoparticles] The polymer nanoparticles obtained by the above manufacturing method have a number-average molecular weight of 5,000 to 60,000 for polyolefin resins, 70,000 to 130,000 for polystyrene resins, 20,000 to 70,000 for polyvinyl chloride resins, and 8,000 to 42,000 for polyester resins. The weight-average molecular weight is 30,000 to 700,000 for polyolefin resins, 150,000 to 350,000 for polystyrene resins, 30,000 to 180,000 for polyvinyl chloride resins, and 30,000 to 80,000 for polyester resins. They are spherical and have an average particle size of less than 1 μm.

[0064] The thermoplastic resin constituting the polymer nanoparticles is the thermoplastic resin used in the dissolution process described above, and is selected from, for example, polyolefin resins, polystyrene resins, polyvinyl chloride resins, and polyester resins.

[0065] Examples of polyolefin resins include high-density polyethylene (HDPE), low-density polyethylene (LDPE), polypropylene (PP), polybutene-1, ethylene-propylene copolymer, ethylene-α-olefin copolymer, and polymer blends thereof.

[0066] Examples of polystyrene-based resins include rubber-reinforced styrene-based resins such as polystyrene (PS), high-impact polystyrene (HIPS), and medium-impact polystyrene, as well as styrene-acrylonitrile copolymer (SAN resin), acrylonitrile-butylacrylate rubber-styrene copolymer (AAS resin), acrylonitrile-ethylenepropyl rubber-styrene copolymer (AES), acrylonitrile-polyethylene chloride-styrene copolymer (ACS), ABS resin (for example, acrylonitrile-butadiene-styrene copolymer, and polymer blends thereof).

[0067] Examples of polyvinyl chloride-based resins include polyvinyl chloride (PVC), polyvinylidene chloride (PVDC), and polymer blends thereof.

[0068] Examples of polyester resins include polyethylene terephthalate (PET), polytrimethylene terephthalate (PTT), polybutylene terephthalate (PBT), polyethylene naphthalate (PEN), polybutylene naphthalate (PBN), and polymer blends thereof.

[0069] Furthermore, polymer nanoparticles made from thermoplastic resins refer to polymer nanoparticles in which, when the impurity concentration is measured using a gas chromatograph-mass spectrometer connected to a pyrolizer, the impurity concentration is 0.01% or less.

[0070] The number-average molecular weight (Mn) of the above polymer nanoparticles is, for example, 5,000 to 60,000, preferably 8,000 to 55,000, for polyolefin resins, 70,000 to 130,000, preferably 90,000 to 120,000, for polystyrene resins, 20,000 to 70,000, preferably 30,000 to 50,000, and for polyester resins, 8,000 to 42,000, preferably 8,000 to 30,000. The weight-average molecular weight (Mw) of the above thermoplastic resins is, for example, 30,000 to 700,000, preferably 70,000 to 300,000 for polyolefin resins, 150,000 to 350,000, preferably 200,000 to 300,000 for polystyrene resins, 30,000 to 180,000, preferably 40,000 to 120,000 for polyvinyl chloride resins, and 30,000 to 80,000, preferably 30,000 to 50,000 for polyester resins. The polydispersity (Mw / Mn) of the above thermoplastic resins is, for example, 1 to 20, preferably 2 to 16. The number-average molecular weight, weight-average molecular weight, and polydispersity of the above thermoplastic resins are measured using an apparatus based on the same principle as HLC-8321GPC / HT (product name of Tosoh Corporation), and the resulting peaks are analyzed. The average molecular weight of polymer nanoparticles falls within this range, making them easy to use as standard materials.

[0071] The polymer nanoparticles have a spherical shape. The average circularity of the polymer nanoparticles is, for example, 0.85 to 1.0, preferably 0.90 to 1.0, and more preferably 0.94 to 1.0 or 0.95 to 1.0. Here, the average circularity is measured by image analysis of scanning electron microscope images of each polymer nanoparticle.

[0072] As mentioned above, the average particle size of polymer nanoparticles is less than 1 μm, preferably 1 to 1000 nm, and more preferably 100 to 500 nm. The average particle size can be obtained, for example, by obtaining scanning electron microscope images at three locations using a scanning electron microscope, measuring the particle size of 150 polymer nanoparticles in the field of view, and calculating the average. Polymer nanoparticles with small particle sizes made of thermoplastic resin are highly useful when used as standard materials, and the average particle size of polymer nanoparticles is preferably 1000 nm or less, and more preferably 500 nm or less.

[0073] The spherical polymer nanoparticles preferably have a symmetrical shape with respect to the particle center and uniform particle size regardless of orientation. When performing filtration or other operations in measurement or analysis, the degree of separation may be measured for each particle size. Using the polymer nanoparticles of this embodiment makes it easy to obtain standard data because the particle size variation is small and the nanoparticles are homogeneous. Furthermore, when conducting toxicity tests, for example, particle shape can be an influencing factor, and it is preferable to have few influencing factors other than composition. Since the polymer nanoparticles of this embodiment are spherical, the influencing factor of shape can be minimized, making it possible to accurately obtain standard toxicity data.

[0074] Furthermore, it is preferable that the polymer nanoparticles have a uniform shape. The polymer nanoparticles are measured by obtaining scanning electron microscope images at three locations using a scanning electron microscope, and measuring the particle size of 150 polymer nanoparticles in the field of view. The coefficient of variation obtained is, for example, 33 to 66%. [Examples]

[0075] The following describes embodiments of the present invention. The present invention is not limited to the following embodiments.

[0076] [Example 1] First, as a dissolution step, 10 mL of xylene (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd., product code: 244-00081), which is the first organic solvent, was prepared and heated to 120°C. 0.02 g of low-density polyethylene (manufactured by Sumitomo Seika Co., Ltd., product code: FG701N), which is a thermoplastic resin, was dissolved in this first organic solvent to form a polymer solution in which the thermoplastic resin content was 0.20% by mass.

[0077] Next, a second organic solvent consisting of dimethyl sulfoxide, which is a good solvent for xylene and a poor solvent for low-density polyethylene, was prepared as a dispersion solvent. In the addition step, 100 mL of this dispersion solvent was maintained at 110°C, and 4 mL of the polymer solution was added to this dispersion solvent using a syringe at an addition rate of 6 mL / min to precipitate the thermoplastic resin. The addition step was carried out while stirring the dispersion solvent with a stirrer.

[0078] Next, the precipitated thermoplastic resin was separated from dimethyl sulfoxide by filtration, and the filtrate was held under vacuum in a centrifugal concentrator (Tommy Seikou Co., Ltd., model number: CC-105) to remove the solvent from the polymer nanoparticles and produce polymer nanoparticles made of low-density polyethylene.

[0079] [Example 2] Polymer nanoparticles made of high-density polyethylene were produced under the same conditions as in Example 1, except that the thermoplastic resin was changed to high-density polyethylene (Alfa Aesar, product code: 41731) and the holding temperature (reaction temperature) of the dispersion solvent was changed to 115°C.

[0080] [Example 3] Polymer nanoparticles made of polypropylene were manufactured under the same conditions as in Example 1, except that the thermoplastic resin was changed to polypropylene (Sigma-Aldrich, product code: 427888).

[0081] [Example 4] Polymer nanoparticles made of polystyrene were produced under the same conditions as in Example 1, except that the thermoplastic resin was changed to polystyrene (Goodfellow Cambridge Ltd., product code: ST313120), the first organic solvent was changed to toluene (Fujifilm Wako Pure Chemical Industries, Ltd., product code: 209-14143), the temperature at which polystyrene was dissolved in toluene was changed to 50°C, the mass percentage of thermoplastic resin in the polymer solution (polymer concentration) was changed to 0.15%, the dispersion solvent was changed to a solvent consisting of water and ethanol, with a water content of 30% by mass relative to the total mass of the dispersion solvent, and the holding temperature of the dispersion solvent (reaction temperature) was changed to 50°C.

[0082] [Example 5] Polymer nanoparticles made of polyvinyl chloride were produced under the same conditions as in Example 1, except that the thermoplastic resin was changed to polyvinyl chloride (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd., product code: 223-00255), the first organic solvent was changed to cyclohexanone (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd., product code: 037-05096), the temperature at which polyvinyl chloride was dissolved in cyclohexanone was changed to 60°C, the mass percentage of thermoplastic resin in the polymer solution (polymer concentration) was changed to 0.40%, the dispersion solvent was changed to a solvent consisting of water and dimethyl sulfoxide, with a water content of 35% by mass relative to the total mass of the dispersion solvent, and the holding temperature of the dispersion solvent (reaction temperature) was changed to 41°C.

[0083] [Example 6] Polymer nanoparticles made of polyethylene terephthalate were produced under the same conditions as in Example 1, except that the thermoplastic resin was changed to polyethylene terephthalate (manufactured by Scientific Polymer Products, Inc., catalog number: 138), the first organic solvent was changed to hexafluoroisopropanol (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd., catalog number: 082-10311), the temperature at which polyethylene terephthalate was dissolved in hexafluoroisopropanol was changed to 45°C, the mass percentage of thermoplastic resin in the polymer solution (polymer concentration) was changed to 1.25%, the dispersion solvent was changed to a solvent consisting of water and acetone, with a water content of 35% by mass relative to the total mass of the dispersion solvent, and the holding temperature of the dispersion solvent (reaction temperature) was changed to 45°C.

[0084] Table 1 summarizes the conditions used to produce polymer nanoparticles in Examples 1 to 6.

[0085] [Table 1]

[0086] (Analysis of polymer nanoparticles) The polymer nanoparticles prepared in Examples 1 to 6 were observed using a scanning electron microscope. Figures 3 to 8 are electron microscope images of polymer nanoparticles prepared in Examples 1 to 6, respectively. As shown in the electron microscope images in Figures 3 to 8, it was confirmed that in Examples 1 to 6, polymer nanoparticles that were mostly spherical, not clumped together, and with a particle size of less than 1 μm could be stably produced.

[0087] For the polymer nanoparticles prepared in Examples 1 to 6, scanning electron microscope images were obtained from three locations within each polymer nanoparticle. The particle size of 150 polymer nanoparticles in the field of view was measured, and the average particle size was calculated. The coefficient of variation for the polymer nanoparticles in Examples 1 to 6 was calculated by dividing the standard deviation of the measured particle sizes by the average particle size.

[0088] (Measurement of average molecular weight) For the polymer nanoparticles prepared in Examples 1 to 3, HLC-8321GPC / HT (product name of Tosoh Corporation) was used, and the column was TSKgelguardcolumnH. HR (30) HT x 1 bottle + TSKgel GMH HR― Three H(20)HT columns (product name of Tosoh Corporation) were used, with 1,2,4-trichlorobenzene as the solvent. The flow rate was set to 1.0 mL / min, the column temperature to 140°C, and the collection time to 0 to 50 minutes. By analyzing the peaks that appeared between 20 and 34 minutes of collection, the number-average molecular weight Mn, weight-average molecular weight Mw, and polydispersity Mw / Mn were determined.

[0089] For the polymer nanoparticles prepared in Examples 4 and 5, HLC-8320GPC (product name of Tosoh Corporation) was used, and the column was TSKgel GMH. HR― Using two H x 2 columns (product name of Tosoh Corporation) and tetrahydrofuran as the solvent, the flow rate was set to 1.0 mL / min, the column temperature to 40°C, and the collection time to 5 to 30 minutes. By analyzing the peaks that appeared between 10 and 18 minutes of collection, the number-average molecular weight Mn, weight-average molecular weight Mw, and polydispersity Mw / Mn were determined.

[0090] For the polymer nanoparticles prepared in Example 6, HLC-8420GPC (Tosoh Corporation product name), two TSKgel Super AWM-H columns (Tosoh Corporation product name), and 1,1,1,3,3,3-hexafluoro-2-propanol were used as the solvent. The flow rate was set to 0.3 mL / min, the column temperature to 40°C, and the collection time to 5 to 25 minutes. The number-average molecular weight Mn, weight-average molecular weight Mw, and polydispersity Mw / Mn were determined by analyzing the peaks that appeared between 10 and 18 minutes of collection.

[0091] (Measurement of average circularity) For the polymer nanoparticles prepared in Examples 1 to 6, scanning electron microscope images were obtained from three locations on each polymer nanoparticle. The circularity of 150 polymer nanoparticles within the field of view was measured using image analysis software (imageJ version 1.53c), and the average circularity was calculated to determine the average.

[0092] (Measurement of production efficiency) In Examples 1-6, the weight of the added thermoplastic resin was determined by multiplying the concentration of the thermoplastic resin in the solution by the weight of the added solution. Furthermore, the polymer nanoparticles prepared in Examples 1-6 were filtered through a 0.25 mm filter and a 2 μm filter to remove coarse particles (aggregates), and the weight of the prepared polymer particles was determined by weighing them using an electronic balance. The production efficiency (%) was calculated by determining the ratio of the weight of the prepared polymer nanoparticles to the weight of the thermoplastic resin added as a raw material.

[0093] Table 2 shows the results of measuring the average particle size, coefficient of variation of average particle size, number-average molecular weight, weight-average molecular weight, polydispersity, and average circularity of the polymer nanoparticles prepared in Examples 1 to 6.

[0094] [Table 2]

[0095] As shown in Table 2, the average particle size of the polymer nanoparticles produced in Examples 1 to 6 was less than 1 μm in all cases. Furthermore, in Examples 1 to 6, the weight-average molecular weight was 3.0 × 10⁶. 4 ~26×10 4 It was confirmed that these were polymer nanoparticles with a large average molecular weight.

[0096] (Analysis of impurity concentration) For the polymer nanoparticles prepared in Examples 1 to 6, a gas chromatograph-mass spectrometer (Shimadzu Corporation, model number: GCMS-QP2010plus) connected to a multi-shot pyrolizer (Frontier Labs Inc., model number: EGA / PY-2020iD) was used, and an Ultra Alloy Capillary column (Frontier Labs Inc., model number: UA5-30M-0.25F) was used to analyze the desorbed products of each polymer nanoparticle at 300°C. The impurity concentration was determined by quantitative analysis using a standard addition method with standard substances. As a result, the impurity concentration of the polymer nanoparticles in Examples 1 to 6 was 0.01% or less in all cases, confirming that the polymer nanoparticles in Examples 1 to 6 were polymer nanoparticles made of thermoplastic resin.

[0097] [Manufacturing Example 1] Polymer nanoparticles made of polystyrene were produced in the same manner as in Example 4, except that a dispersion solvent consisting solely of ethanol was used.

[0098] [Manufacturing Example 2] Polymer nanoparticles made of polystyrene were produced in the same manner as in Example 4, except that the ratio of water in the dispersion solvent was changed to 35% by mass.

[0099] [Manufacturing Example 3] Polymer nanoparticles made of polyvinyl chloride were produced in the same manner as in Example 5, except that the ratio of water in the dispersion solvent was changed to 20% by mass.

[0100] [Manufacturing Example 4] Polymer nanoparticles made of polyvinyl chloride were produced in the same manner as in Example 5, except that the ratio of water in the dispersion solvent was changed to 37.5% by mass.

[0101] [Example 7] Polymer nanoparticles made of polyethylene terephthalate were produced in the same manner as in Example 6, except that the ratio of water in the dispersion solvent was changed to 40% by mass.

[0102] [Manufacturing Example 5] Polymer nanoparticles made of polyethylene terephthalate were produced in the same manner as in Example 6, except that the ratio of water in the dispersion solvent was changed to 60% by mass.

[0103] [Comparative Example 1] Polymer nanoparticles made of polyethylene terephthalate were produced in the same manner as in Example 6, except that the dispersion solvent was changed to a dispersion solvent consisting only of water.

[0104] Table 3 summarizes the conditions used to produce polymer nanoparticles in Example 7, Production Examples 1-5, and Comparative Example 1.

[0105] [Table 3]

[0106] Figures 9 to 12 show electron microscope images of polymer particles produced in Production Examples 1 to 4, observed using a scanning electron microscope. In Production Example 1, the polymer nanoparticles were observed to be generally attached to surrounding polymer nanoparticles. In Production Example 2, the polymer particles were observed to form clumps of approximately 10 μm in size. This is thought to be due to the poor dispersibility of toluene, the primary organic solvent, in water. In Production Example 3, the polymer nanoparticles were observed to be generally attached to surrounding polymer nanoparticles. In Production Example 4, some polymer nanoparticles were observed to have an elongated shape. This is thought to be due to the poor dispersibility of cyclohexanone, the primary organic solvent, in water. The average circularity of the polymer nanoparticles in Production Example 4 was measured using the same method as in Examples 1 to 6 and was found to be 0.56.

[0107] When polymer nanoparticles prepared in Example 7, Production Example 5, and Comparative Example 1, using polyethylene terephthalate as the thermoplastic resin, were observed, aggregated particles of several millimeters in size were found in Comparative Example 1. In contrast, in Example 7, almost no aggregated particles of several millimeters in size were observed, and almost all particles were on the nanometer scale, confirming that nanoparticles could be produced stably.

[0108] The production efficiency of polymer nanoparticles prepared in Example 7, Production Example 5, and Comparative Example 1, using polyethylene terephthalate as the thermoplastic resin, was determined using the same method as in Examples 1 to 6. As a result, the production efficiencies of the polymer nanoparticles in Example 7, Production Example 5, and Comparative Example 1 were 100%, 37%, and 93%, respectively. It was confirmed that Examples 6 and 7 had higher production efficiencies than Comparative Example 1, which used only water as the dispersion solvent, while Production Example 5, which used a liquid consisting of water and a small amount of organic solvent as the dispersion solvent, had lower production efficiency than Comparative Example 1.

[0109] Figures 13, 14, and 15 show images of the filters used to determine the production efficiency in Example 7, Production Example 5, and Comparative Example 1, respectively. It was confirmed that in experiments with lower production efficiency, larger, aggregated clumps of particles were removed by the filter. [Industrial applicability]

[0110] The present invention provides a method for producing polymer nanoparticles that enables the safe and stable production of uniform and homogeneous polymer nanoparticles suitable for use as standard material particles. Furthermore, the polymer nanoparticles obtained by this method are useful as standard materials for measurement or analysis, and are particularly useful for measuring the abundance of nanoplastics in the ocean and evaluating their toxicity, which has been difficult to assess due to the lack of nano-order-size standard materials. Furthermore, the polymer nanoparticles obtained by this manufacturing method consist of a predetermined thermoplastic resin, including general-purpose engineering plastics, and are uniform in shape and particle size. They are expected to be utilized not only in measurement and analysis but also in various industries such as manufacturing. [Explanation of Symbols]

[0111] 10: Container, 20: Dispersion solvent, 30: Stirrer, 40: Cylinder, 50: Polymer solution, 60: Polymer nanoparticles

Claims

1. A polymer nanoparticle that is spherical and has an average particle size of less than 1 μm, The average particle size is obtained by using a scanning electron microscope to acquire scanning electron microscope images at three locations, measuring the particle size of 150 polymer nanoparticles in the field of view, and calculating the average. The polymer nanoparticles consist of a thermoplastic resin, which is a polyolefin resin, a polystyrene resin, a polyvinyl chloride resin, or a polyester resin. When the thermoplastic resin is a polyolefin resin, the weight-average molecular weight Mw of the polymer nanoparticles is 30,000 to 700,000. When the thermoplastic resin is a polystyrene-based resin, the weight-average molecular weight Mw of the polymer nanoparticles is 150,000 to 350,000. When the thermoplastic resin is a polyvinyl chloride resin, the weight-average molecular weight Mw of the polymer nanoparticles is 30,000 to 180,000. When the thermoplastic resin is a polyester resin, the weight-average molecular weight Mw of the polymer nanoparticles is 30,000 to 80,000. The polymer nanoparticles have a polydispersity degree Mw / Mn of 1 to 20.

2. Polymer nanoparticles according to claim 1, When the thermoplastic resin is a polyolefin resin, the weight-average molecular weight Mw of the polymer nanoparticles is 70,000 to 300,000. When the thermoplastic resin is a polystyrene-based resin, the weight-average molecular weight Mw of the polymer nanoparticles is 200,000 to 300,000. When the thermoplastic resin is a polyvinyl chloride resin, the weight-average molecular weight Mw of the polymer nanoparticles is 40,000 to 120,000. When the thermoplastic resin is a polyester resin, the weight-average molecular weight Mw of the polymer nanoparticles is 30,000 to 50,000. The polymer nanoparticles have a polydispersity degree Mw / Mn of 2 to 16.

3. The polymer nanoparticles according to claim 1 or 2, wherein the average circularity of the polymer nanoparticles is 0.85 to 1.

0.

4. The polymer nanoparticles according to claim 1 or 2, wherein the average circularity of the polymer nanoparticles is 0.90 to 1.

0.

5. The polymer nanoparticles according to claim 1 or 2, wherein the average circularity of the polymer nanoparticles is 0.94 to 1.

0.

6. The polymer nanoparticles according to claim 1 or 2, wherein the average circularity of the polymer nanoparticles is 0.95 to 1.

0.

7. The polymer nanoparticles according to claim 1 or 2, wherein the average particle size is 1 to 1000 nm.

8. The polymer nanoparticles according to claim 1 or 2, wherein the average particle size is 100 to 500 nm.

9. The polymer nanoparticles according to claim 1 or 2, wherein scanning electron microscope images are obtained at three locations using a scanning electron microscope, the particle size of 150 polymer nanoparticles in the field of view is measured, and the coefficient of variation obtained is 33 to 66%.

10. Polymer nanoparticles according to claim 1 or 2, used as a standard material for measurement or analysis.

Images