Analytical cover film, analytical component, and analytical method

Abstract

The present invention provides an analytical cover film, analytical component, and analytical method that enable effective optical analysis. [Solution] An analytical cover film 1 to be attached to an analytical substrate 2 having a containment section 4 for containing an object to be analyzed and an adhesive application section 3, comprising a base film 10 and an adhesive layer 20 made of a pressure-sensitive adhesive laminated on one side of the base film 10, wherein the adhesive layer 20 is laminated on the base film 10 so as to face the containment section 4 and the adhesive application section 3 of the analytical substrate 2, and the analytical cover film 1 has a haze value of 1% or less as measured by a haze meter.

Description

Technical Field

[0001] The present invention relates to an analysis member used for optically analyzing an analyte, an analysis cover film used for the analysis member, and an analysis method using the analysis member.

Background Art

[0002] There are methods for optically analyzing a specimen for the purpose of measuring the turbidity in a specimen such as a liquid or measuring the amount of a specific component in the specimen. In such an optical analysis method, light is irradiated onto the specimen, and the light generated thereby is measured. More specific examples of such an analysis method include a method of irradiating light onto a specimen and measuring the degree of scattering of the light, a method of irradiating light onto a specimen and measuring the amount of light absorbed by components in the specimen when the light passes through the specimen, a method of measuring fluorescence generated in the specimen, and the like. In such an analysis method, conventionally, a specimen has been accommodated in a test tube or a cell having a volume of several milliliters.

[0003] In recent years, an analysis method using an analysis member provided with a fine flow path for accommodating a specimen has been developed instead of the above test tube or cell. In such a method, even a small amount of specimen can be analyzed, so that the amount of specimen required for analysis can be very small. Furthermore, by using an analysis member having a plurality of grooves, intensive measurement can be performed, so that a large number of specimens can be analyzed simultaneously.

[0004] As an example of the analysis member having the above-mentioned fine flow path, Patent Document 1 discloses a multilayer composite structure including a base material constituting the side surface of a groove, a first layer constituting the bottom surface of the groove, and a resealable film serving as a cover for covering the groove (paragraph 0032 and FIG. 2 of Patent Document 1). Further, Patent Document 2 discloses a plastic microchip formed by bonding a plastic substrate having the above groove as a fine flow path on the surface and a plastic film serving as a cover for covering the groove via an adhesive (claim 1 and paragraph 0019 of Patent Document 2).

Prior Art Documents

[0005] [Patent Document 1] Special Publication No. 2006-510384 [Patent Document 2] Japanese Patent Publication No. 2008-157644 [Overview of the project] [Problems that the invention aims to solve]

[0006] Incidentally, as is also the case with the analytical members described in Patent Documents 1 and 2, the grooves may be covered with a film to prevent evaporation, leakage, and contamination of the sample.

[0007] When performing optical analysis using the above-mentioned analytical components, external light passes through the film and irradiates the sample, or light emitted from the sample passes through the film and is measured. However, depending on the composition, type, and condition of the film, light scattering may occur, making it impossible to perform a good analysis.

[0008] This invention has been made in view of the above-described circumstances, and aims to provide an analytical cover film, an analytical member, and an analytical method that enable good optical analysis. [Means for solving the problem]

[0009] To achieve the above objective, firstly, the present invention provides an analytical cover film to be attached to an analytical substrate having a containment portion for containing an analyte and an adhesive application portion, comprising a base film and an adhesive layer made of a pressure-sensitive adhesive laminated on one side of the base film, wherein the adhesive layer is laminated on the base film so as to face the containment portion and the adhesive application portion of the analytical substrate, and the haze value measured by a haze meter is 1% or less (Invention 1).

[0010] In the above invention (Invention 1), when light is irradiated onto the object to be analyzed contained in the groove 4 via the analytical cover film, or when light emitted from the object to be analyzed contained in the containment section is acquired, light scattering is suppressed, excellent light transmittance is obtained, and optical analysis can be performed with high accuracy.

[0011] In the above invention (Invention 1), it is preferable that the arithmetic mean roughness Ra of the side of the adhesive layer that is attached to the analytical substrate is 14 nm or less (Invention 2).

[0012] In the above inventions (Inventions 1 and 2), a release sheet is laminated on the adhesive layer, and it is preferable that the arithmetic mean roughness Ra of the surface of the release sheet in contact with the adhesive layer is 15 nm or less (Invention 3).

[0013] In the above inventions (Inventions 1 to 3), it is preferable that the base film contains one or more resins selected from the group consisting of cycloolefin copolymers and polycarbonates (Invention 4).

[0014] In the above inventions (Inventions 1 to 4), it is preferable that a hard coat layer is formed on the surface of the base film opposite to the adhesive layer (Invention 5).

[0015] Secondly, the present invention provides an analytical member comprising an analytical substrate having a containment portion for containing an object to be analyzed and an adhesive portion, and an analytical cover film (Inventions 1 to 5) attached to the analytical substrate, wherein a flow path is formed when the opening of the containment portion is sealed with the analytical cover film (Invention 6).

[0016] Thirdly, the present invention provides an analytical method (Invention 7) which involves preparing the analytical member (Invention 6), composing a fluid containing the object to be analyzed in the flow path of the analytical member, and analyzing the object to be analyzed using light transmitted through the analytical cover film. [Effects of the Invention]

[0017] According to the cover film for analysis, analysis member, and analysis method according to the present invention, optical analysis can be performed well.

Brief Description of the Drawings

[0018] [Figure 1] It is a cross-sectional view of a cover film for analysis according to an embodiment of the present invention. [Figure 2] It is a cross-sectional view of an analysis member according to an embodiment of the present invention.

Mode for Carrying Out the Invention

[0019] Hereinafter, embodiments of the present invention will be described. In addition, the term "analysis" in this specification is assumed to include the concept of "inspection".

[0020] 〔Cover Film for Analysis〕 In FIG. 1, a cover film 1 for analysis according to an embodiment of the present invention is shown. This cover film 1 for analysis includes a base film 10 and an adhesive layer 20 made of a pressure-sensitive adhesive laminated on one side of the base film 10. In the cover film 1 for analysis according to the present embodiment, the adhesive layer 20 constitutes the outermost layer on one side of the cover film 1 for analysis.

[0021] The analytical cover film 1 according to this embodiment is for constructing an analytical member used for analysis, and preferably for constructing an analytical member 100 as shown in Figure 2. The analytical member 100 comprises an analytical substrate 2 and an analytical cover film 1 attached to the analytical substrate 2. The analytical substrate 2 has a groove 4 as a housing for accommodating an object to be analyzed (for example, a specimen), and a planar adhesive application area 3. The adhesive layer 20 of the analytical cover film 1 is laminated on the base film 10 so as to face the groove 4 and the adhesive application area 3. The analytical cover film 1 is attached to the analytical substrate 2 by the adhesive layer 20 of the analytical cover film 1 being attached to the adhesive application area 3. The groove 4 of the analytical substrate 2 has its opening (the upper surface of the groove 4 in Figure 2) sealed by the analytical cover film 1, thereby forming a flow path. In this analytical member 100, optical analysis is performed on the object to be analyzed contained in the groove 4.

[0022] The haze value measured by the haze meter on the analytical cover film 1 is 1% or less. This suppresses light scattering when irradiating the analyte contained in the groove 4 with light through the analytical cover film 1, or when acquiring light emitted by the analyte contained in the groove 4, resulting in excellent light transmittance and enabling accurate optical analysis. For example, it allows for good optical detection of target substances by irradiation with laser light or LED light, and also enables fluorescence detection of target substances. The light used in the analysis may be referred to as "analytical light" below.

[0023] From the viewpoint of suppressing light scattering, the haze value measured by a haze meter on the analytical cover film 1 is preferably 0.8% or less, and particularly preferably 0.5% or less. While there is no particular lower limit to the haze value, it is usually 0% or higher. The haze values ​​used herein are measured in accordance with JIS K7136:2000, and the specific test methods are as shown in the test examples described later.

[0024] The analytical cover film 1 according to this embodiment includes an adhesive layer 20 made of a pressure-sensitive adhesive, allowing it to be easily attached to the analytical substrate 2 without requiring a heating process. Furthermore, it prevents problems such as the viscosity of the adhesive (especially thermoplastic adhesives) decreasing due to the heating process and the adhesive getting into the grooves 4. In addition, the adhesive layer 20 is laminated on the base film 10 so as to face the grooves 4 and adhesive application area 3 of the analytical substrate 2, and in this embodiment in particular, it is laminated over the entire surface of one side of the base film 10. Therefore, when manufacturing the analytical cover film 1, there is no need for processes such as not forming the adhesive layer 20 corresponding to the grooves 4, or removing the formed adhesive layer 20 corresponding to the grooves 4, allowing the analytical cover film 1 to be manufactured easily and at low cost.

[0025] 1. Components of the analytical cover film 1-1. Base film In this embodiment, the base film 10 only needs to be transparent to analytical light and capable of ensuring that the analytical cover film 1 satisfies the aforementioned haze value, and it is preferable that it has optical isotropy.

[0026] The base film 10 can be made of resin, glass, or the like, but resin is preferred because it is easy to manufacture and handle. In other words, the base film 10 is preferably a resin film.

[0027] Examples of the above-mentioned resins include polyesters such as polyethylene terephthalate, polybutylene terephthalate, and polyethylene naphthalate; polyolefins such as polyethylene and polypropylene; polycarbonate, cycloolefin copolymer, diacetylcellulose, triacetylcellulose, acetylcellulose butyrate, polyvinyl chloride, polyvinylidene chloride, polyvinyl alcohol, ethylene-vinyl acetate copolymer, polystyrene, polymethylpentene, polysulfone, polyetheretherketone, polyethersulfone, polyetherimide, polyimide, polyamide, acrylic resin, norbornene-based resin, fluororesin, polyphenylene sulfide, and liquid crystal polymer. Resin films using these resins may be single layers or laminates of the same or different types of resins.

[0028] The above-mentioned resin film is preferably an unstretched film from the viewpoint of optical isotropy. Furthermore, among resin films, polycarbonate film, cycloolefin copolymer film, polyethylene terephthalate film, polybutylene terephthalate film, or acrylic resin film is preferred from the viewpoint of excellent transmittance to analytical light and ease of satisfying the haze value mentioned above, and in particular, cycloolefin copolymer film or polycarbonate film is preferred. Details of the analytical light will be described later.

[0029] Furthermore, it is preferable that the material of the base film 10 is the same as the material of the analytical substrate 2, which will be described later. By using the same material, the difference in transmittance to analytical light between the base film 10 and the analytical substrate 2 can be reduced, and the influence of such differences on the measurement can be reduced.

[0030] The glass transition temperature (Tg) of the material constituting the base film 10 is preferably 100°C or higher, particularly preferably 120°C or higher, and even more preferably 140°C or higher. This makes it difficult for the base film 10 to melt even if the analytical member 100 is heated during analysis, effectively suppressing deformation of the base film 10 and effectively suppressing peeling and shifting at the interface between the base film 10 and the adhesive layer 20. Although there is no particular upper limit to the glass transition temperature (Tg) of the material constituting the base film 10, from the viewpoint of flexibility for attaching the analytical cover film 1, it is usually preferably 300°C or lower, particularly preferably 250°C or lower, and even more preferably 200°C or lower.

[0031] In the base film 10, it is preferable to form a hard coat layer on the side opposite to the adhesive layer 20. This improves the smoothness of the side of the base film 10 opposite to the adhesive layer 20, making it easier to satisfy the aforementioned haze value. It also prevents scratches on this surface, thus suppressing a decrease in the haze value of the analytical cover film 1. In particular, the formation of a hard coat layer is effective for cycloolefin copolymer films and polycarbonate films, as their surfaces are easily scratched.

[0032] The hard coat layer can be formed from known materials, and its thickness is not particularly limited and can be of a general thickness. For example, active energy ray curable components such as polyfunctional acrylate monomers and acrylate prepolymers can be used as materials. The thickness can be, for example, about 0.5 to 20 μm.

[0033] In the base film 10, surface treatments such as oxidation or embossing, or primer treatments, can be applied to the surface facing the adhesive layer 20, in order to improve adhesion to the adhesive layer 20, provided that the transmittance to analytical light is not impaired. Examples of oxidation methods include corona discharge treatment, plasma discharge treatment, chromium oxidation treatment (wet), flame treatment, hot air treatment, ozone, and ultraviolet irradiation treatment. Examples of embossing methods include sandblasting and thermal spraying. These surface treatment methods are appropriately selected depending on the type of material constituting the base film 10. Among these, oxidation or primer treatment is preferred from the viewpoint of maintaining the smoothness of the base film 10.

[0034] The thickness of the base film 10 is preferably 30 μm or more, particularly preferably 50 μm or more, even more preferably 75 μm or more, and most preferably 90 μm or more. This ensures that the base film 10 has sufficient strength, is easy to handle, and suppresses deformation and damage to the base film 10 when using the analytical member 100. Furthermore, the thickness is preferably 300 μm or less, particularly preferably 200 μm or less, even more preferably 150 μm or less, and most preferably 110 μm or less. This makes it easier for the base film 10 to have excellent transmittance to analytical light, and facilitates good analysis with the resulting analytical member 100.

[0035] The transmittance of analytical light through the base film 10 is preferably 60% or higher, particularly preferably 80% or higher, and even more preferably 90% or higher. This allows the analytical cover film 1 to have better transmittance to analytical light, enabling more accurate analysis. The upper limit of the above transmittance is not particularly limited, but is 100% or less.

[0036] The arithmetic mean roughness Ra of the surface of the base film 10 opposite to the adhesive layer 20 is preferably 200 nm or less, particularly preferably 100 nm or less, and even more preferably 50 nm or less. This makes it easier for the above haze value of the analytical cover film 1 to be satisfied. The lower limit of the arithmetic mean roughness Ra is not particularly limited, but is usually preferably 0.1 nm or more, particularly preferably 0.5 nm or more, and even more preferably 1 nm or more.

[0037] The arithmetic mean roughness Ra of the surface of the base film 10 opposite to the adhesive layer 20 can be obtained by fixing the analytical cover film 1 to the glass plate via double-sided tape so that the side of the analytical cover film 1 facing the adhesive layer 20 faces the glass plate, and then measuring the surface of the analytical cover film 1 facing the base film 10 using a surface roughness measuring instrument (Mitutoyo Corporation, product name "rSV-3000S4", stylus type) in accordance with JIS B0601:2013.

[0038] 1-2. Adhesive layer The adhesive layer 20 of the analytical cover film 1 according to this embodiment is not particularly limited, as long as the haze value of the analytical cover film 1 satisfies the aforementioned value, and it can securely fix the analytical cover film 1 and the analytical substrate 2 without adversely affecting optical analysis.

[0039] The adhesive constituting the adhesive layer 20 may be any adhesive that satisfies the above conditions, and its type is not particularly limited. For example, it may be an acrylic adhesive, polyester adhesive, polyurethane adhesive, rubber adhesive, silicone adhesive, etc. Furthermore, the adhesive may be an emulsion type, solvent type, or solvent-free type, and may be a crosslinked type or a non-crosslinked type, but a crosslinked type is preferred as it is easier to obtain cohesiveness of the adhesive layer 20. Among these, an acrylic adhesive is preferred as it has excellent adhesive properties, optical properties, etc. As for acrylic adhesives, a crosslinked type is preferred, and a thermally crosslinked type is even more preferred. Furthermore, the adhesive in this embodiment may be non-curable by active energy rays or curable by active energy rays.

[0040] The adhesive constituting the adhesive layer 20 is preferably obtained from an adhesive composition (hereinafter sometimes referred to as "adhesive composition P"), and more specifically, it is preferably obtained from an adhesive composition P containing a polymer (A). Furthermore, if the adhesive constituting the adhesive layer 20 is of the crosslinking type, an adhesive layer obtained from an adhesive composition P containing a polymer (A) and a crosslinking agent (B) is preferred, and the adhesive composition P may optionally contain an active energy ray curable component (C). Note that the concept of "polymer" also includes the concept of "polymer".

[0041] (1) Each component (1-1) Polymer (A) Examples of polymer (A) include (meth)acrylic acid ester polymers, polyolefins, polyurethanes, and rubber-based polymers, but (meth)acrylic acid ester polymer (A1) is preferred from the viewpoint of easily adjusting various physical properties. In this specification, (meth)acrylic acid means both acrylic acid and methacrylic acid. The same applies to other similar terms. (Meth)acrylic acid ester polymer (A1) preferably contains a component derived from alkyl (meth)acrylate ester. This allows for the expression of good tackiness. The alkyl group may be linear or branched.

[0042] From the viewpoint of adhesiveness, alkyl (meth)acrylate esters with 1 to 20 carbon atoms in the alkyl group are preferred, and from the viewpoint of further improving adhesiveness, alkyl (meth)acrylate esters with 4 to 8 carbon atoms in the alkyl group are preferred. Examples of alkyl (meth)acrylate esters with 4 to 8 carbon atoms in the alkyl group include n-butyl (meth)acrylate, n-pentyl (meth)acrylate, n-hexyl (meth)acrylate, 2-ethylhexyl (meth)acrylate, and isooctyl (meth)acrylate. Among these, n-butyl (meth)acrylate, 2-ethylhexyl (meth)acrylate, or isooctyl (meth)acrylate are particularly preferred. These may be used individually or in combination of two or more.

[0043] The (meth)acrylic acid ester polymer (A1) preferably contains a component derived from a reactive group-containing monomer that has a reactive group within its molecule that reacts with the crosslinking agent (B). The reactive group derived from this reactive group-containing monomer reacts with the crosslinking agent (B) to form a crosslinked structure (three-dimensional network structure), resulting in an adhesive with the desired cohesive force.

[0044] Preferred reactive group-containing monomers include monomers having a hydroxyl group in the molecule (hydroxyl group-containing monomers) and monomers having a carboxyl group in the molecule (carboxyl group-containing monomers). Reactive group-containing monomers can be used individually or in combination of two or more. Among these, hydroxyl group-containing monomers or carboxyl group-containing monomers that exhibit excellent reactivity with the crosslinking agent (B) are preferred, and using both in combination is also preferable.

[0045] As the hydroxyl group-containing monomer, hydroxyalkyl (meth)acrylate esters having a hydroxyalkyl group with 1 to 4 carbon atoms are preferred from the viewpoint of reactivity with the crosslinking agent (B) and copolymerizability with other monomers. Examples include 2-hydroxyethyl (meth)acrylate, 2-hydroxypropyl (meth)acrylate, 3-hydroxypropyl (meth)acrylate, 2-hydroxybutyl (meth)acrylate, 3-hydroxybutyl (meth)acrylate, and 4-hydroxybutyl (meth)acrylate. These may be used individually or in combination of two or more.

[0046] Examples of carboxyl group-containing monomers include ethylenically unsaturated carboxylic acids such as acrylic acid, methacrylic acid, crotonic acid, maleic acid, itaconic acid, and citraconic acid. Among these, acrylic acid is preferred in terms of the reactivity of the carboxyl group in the resulting (meth)acrylic acid ester polymer (A1) with the crosslinking agent (B) and copolymerizability with other monomers. These may be used individually or in combination of two or more.

[0047] The (meth)acrylic acid ester polymer (A1) preferably contains 0.5 to 20% by mass of a component derived from a reactive group-containing monomer, and more preferably 1 to 10% by mass. This results in the formation of a good crosslinking structure in the resulting adhesive, and a suitable cohesive force is obtained.

[0048] The (meth)acrylic acid ester polymer (A1) may optionally contain components derived from other monomers. Examples of other monomers include alicyclic monomers such as isobornyl (meth)acrylate, nitrogen atom-containing monomers such as N-(meth)acryloylmorpholine, alkoxyalkyl (meth)acrylates such as methoxyethyl (meth)acrylate and ethoxyethyl (meth)acrylate, vinyl acetate, and styrene. These may be used individually or in combination of two or more.

[0049] The (meth)acrylic acid ester polymer (A1) is preferably a linear polymer. Being a linear polymer makes it easier for molecular chains to intertwine, which can be expected to improve cohesiveness.

[0050] The (meth)acrylic acid ester polymer (A1) may be polymerized in solution, without solvents, or by emulsion polymerization. Among these, a solution polymer obtained by solution polymerization is preferred. Being a solution polymer makes it easier to obtain a high molecular weight polymer, resulting in an adhesive with excellent durability.

[0051] The polymerization mode of the (meth)acrylic acid ester polymer (A1) may be a random copolymer or a block copolymer.

[0052] The weight-average molecular weight of the (meth)acrylic acid ester polymer (A1) is preferably 10 to 3 million, and more preferably 300,000 to 2.5 million. This tends to result in a suitable adhesive strength for the resulting adhesive. Here, the weight-average molecular weight as used herein is the value on a standard polystyrene basis measured by gel permeation chromatography (GPC).

[0053] In the adhesive composition P, the (meth)acrylic acid ester polymer (A1) may be used alone or in combination of two or more types.

[0054] (1-2) Crosslinking agent (B) The crosslinking agent (B) can be any agent that can react with the reactive groups of the polymer (A). Examples of crosslinking agents (B) include isocyanate crosslinking agents, epoxy crosslinking agents, amine crosslinking agents, melamine crosslinking agents, aziridine crosslinking agents, hydrazine crosslinking agents, aldehyde crosslinking agents, oxazoline crosslinking agents, metal alkoxide crosslinking agents, metal chelate crosslinking agents, metal salt crosslinking agents, and ammonium salt crosslinking agents. The crosslinking agent (B) can be used alone or in combination of two or more types.

[0055] When polymer (A) has a hydroxyl group as a reactive group, it is preferable to use an isocyanate-based crosslinking agent that has high reactivity with the hydroxyl group. When polymer (A) has a carboxyl group as a reactive group, it is preferable to use an epoxy-based crosslinking agent or an isocyanate-based crosslinking agent that has high reactivity with the carboxyl group. Isocyanate-based crosslinking agents and epoxy-based crosslinking agents can also be used in combination.

[0056] The content of the crosslinking agent (B) in the adhesive composition P is preferably 0.05 to 10 parts by mass, and more preferably 0.1 to 8 parts by mass, per 100 parts by mass of the (meth)acrylic acid ester polymer (A1). This makes it easier to obtain an adhesive with suitable cohesive force and tackiness.

[0057] (1-3) Active energy ray curing component (C) The adhesive composition P may also preferably contain an active energy ray curable component (C). As a result, the resulting adhesive is cured by active energy rays and has excellent cohesive properties, making it difficult for the adhesive layer to penetrate the grooves of the analytical substrate, thus preventing a reduction in the cross-sectional area of ​​the flow path.

[0058] The active energy ray curable component (C) is not particularly limited as long as it hardens upon irradiation with active energy rays, and is preferably a compound having functional groups that can react with each other upon irradiation with active energy rays, such as vinyl groups, (meth)acryloyl groups, epoxy groups, and oxetanyl groups. The active energy ray curable component (C) may be a monomer, oligomer, or polymer, or a mixture thereof, and the polymer (A) described above may have the property of hardening upon irradiation with active energy rays and also serve as the active energy ray curable component (C). Among these, polyfunctional acrylate monomers that have superior adhesive strength after hardening are particularly preferred.

[0059] As the polyfunctional acrylate monomer, 1- to 6-functional acrylate monomers are preferred, and 2- to 3-functional acrylate monomers are more preferred. Examples of 2- to 3-functional acrylate monomers include ethylene oxide-modified isocyanuric acid diacrylate, ethylene oxide-modified isocyanuric acid triacrylate, and dicyclopentanyl di(meth)acrylate. These may be used individually or in combination of two or more. Furthermore, from the viewpoint of compatibility with other compounding materials such as polymer (A), polyfunctional acrylate monomers with a molecular weight of less than 5000 are preferred, those with a molecular weight of less than 3000 are more preferred, and those with a molecular weight of less than 1000 are particularly preferred.

[0060] When the adhesive composition P contains a polymer (A) and an active energy ray curable component (C), that is, when the adhesive composition P contains an active energy ray curable component (C) separately from the polymer (A), the content of the active energy ray curable component (C) in the adhesive composition P is preferably 3 to 100 parts by mass, and particularly preferably 5 to 50 parts by mass, per 100 parts by mass of polymer (A), from the viewpoint of improving the adhesive strength of the resulting adhesive.

[0061] (1-4) Photopolymerization initiator (D) When the adhesive composition P contains an active energy ray-curable component (C) and ultraviolet light is used as the active energy ray, it is preferable to include a photopolymerization initiator (D). By including a photopolymerization initiator (D), the active energy ray-curable component (C) can be cured efficiently, and the polymerization curing time and the amount of ultraviolet light irradiation can be reduced. As such a photopolymerization initiator (D), photoradical polymerization initiators, photocationic polymerization initiators, photoanionic polymerization initiators, etc., can be used.

[0062] The content of the photopolymerization initiator (D) in the adhesive composition P is preferably 2 to 20 parts by mass, particularly preferably 4 to 18 parts by mass, and even more preferably 6 to 15 parts by mass, per 100 parts by mass of the active energy ray curable component (C).

[0063] (1-5) Various additives The adhesive composition P may optionally contain various additives commonly used in acrylic adhesives, such as silane coupling agents, ultraviolet absorbers, infrared absorbers, refractive index modifiers, antistatic agents, colorants, tackifiers, rust inhibitors, antioxidants, light stabilizers, and softeners. The polymerization solvent and diluent solvent described later are not included in the additives constituting the adhesive composition P.

[0064] (2) Preparation of adhesive composition The adhesive composition P can be prepared, for example, by producing a (meth)acrylic acid ester polymer (A1) and mixing the obtained (meth)acrylic acid ester polymer (A1) with other components.

[0065] (Meth)acrylic acid ester polymer (A1) can be produced by polymerizing a mixture of monomers constituting the polymer using a conventional radical polymerization method.

[0066] Once the (meth)acrylic acid ester polymer (A1) is obtained, a crosslinking agent (B), and optionally an active energy ray curable component (C), a photopolymerization initiator (D), additives, a diluent, etc., are added to the solution of the (meth)acrylic acid ester polymer (A1), and thoroughly mixed to obtain a solvent-diluted adhesive composition P (coating solution). If any of the above components are used in solid form, or if precipitation occurs when mixed with other components in an undiluted state, that component may be dissolved or diluted in a diluent solvent beforehand before mixing with the other components.

[0067] Examples of the diluent solvents used include aliphatic hydrocarbons such as hexane, heptane, and cyclohexane; aromatic hydrocarbons such as toluene and xylene; halogenated hydrocarbons such as methylene chloride and ethylene chloride; alcohols such as methanol, ethanol, propanol, butanol, and 1-methoxy-2-propanol; ketones such as acetone, methyl ethyl ketone, 2-pentanone, isophorone, and cyclohexanone; esters such as ethyl acetate and butyl acetate; and cellosolve solvents such as ethyl cellosolve.

[0068] 2. Method for manufacturing analytical cover film A preferred method for manufacturing the analytical cover film 1 involves first forming an adhesive layer 20 using an adhesive composition P.

[0069] (1) Formation of the adhesive layer An adhesive layer is obtained by applying the adhesive composition P (coating liquid) to a desired object, preferably the release surface of a release sheet, and then crosslinking it as desired. The adhesive composition P may be one obtained by the preparation method described above, or it may be another adhesive composition, but the adhesive composition P obtained by the preparation method described above will be explained below as an example.

[0070] To form the adhesive layer 20, it is preferable to apply the coating solution of the adhesive composition P to the release surface of the release sheet and perform a heat treatment to thermally crosslink the adhesive composition P, and it is also preferable to irradiate it with active energy rays.

[0071] Thermal crosslinking of the adhesive composition P can be performed by heat treatment. This heat treatment can also be combined with the drying treatment after application of the adhesive composition P. The heating temperature for the heat treatment is preferably 50 to 150°C, and particularly preferably 70 to 120°C. The heating time is preferably 10 seconds to 10 minutes, and particularly preferably 50 seconds to 2 minutes. After the heat treatment, a curing period of 1 to 2 weeks at room temperature (e.g., 23°C, 50% RH) may be provided as needed. If a curing period is necessary, the adhesive will be formed after the curing period has elapsed; if a curing period is not necessary, the adhesive will be formed after the heat treatment is completed.

[0072] The above heat treatment (and curing) forms a crosslinked product of the (meth)acrylic acid ester polymer (A1) crosslinked with the crosslinking agent (B).

[0073] In this specification, the term "peel surface" of a release sheet refers to the surface of the release sheet that exhibits peelability, and includes both surfaces that have undergone a peeling treatment and surfaces that exhibit peelability even without a peeling treatment.

[0074] Methods for applying the coating solution of the above-mentioned adhesive composition P include, for example, bar coating, knife coating, roll coating, blade coating, die coating, and gravure coating.

[0075] Examples of release sheets include polyethylene film, polypropylene film, polybutene film, polybutadiene film, polymethylpentene film, polyvinyl chloride film, vinyl chloride copolymer film, polyethylene terephthalate film, polyethylene naphthalate film, polybutylene terephthalate film, polyurethane film, ethylene vinyl acetate film, ionomer resin film, ethylene-(meth)acrylic acid copolymer film, ethylene-(meth)acrylic acid ester polymer film, polystyrene film, polycarbonate film, polyimide film, fluororesin film, etc. Crosslinked films of these materials are also used. Furthermore, laminated films of these materials may also be used.

[0076] It is preferable that the release surface (the surface in contact with the adhesive layer 20) of the above-mentioned release sheet is subjected to a release treatment. Examples of release agents used in the release treatment include alkyd, silicone, fluorine, unsaturated polyester, polyolefin, and wax-based release agents.

[0077] The arithmetic mean roughness Ra of the release surface (the surface in contact with the adhesive layer 20) of the release sheet is preferably 15 nm or less, more preferably 8 nm or less, and particularly preferably 5 nm or less. This allows the surface shape of the release surface to be transferred to the side of the adhesive layer 20 that is attached to the analytical substrate 2 (the adhesive surface), resulting in a smoother adhesive surface of the adhesive layer 20, making it easier for the adhesive surface of the adhesive layer 20 to satisfy the arithmetic mean roughness Ra described later. The lower limit of the arithmetic mean roughness Ra is not particularly limited, but is usually preferably 0.5 nm or more, and particularly preferably 1 nm or more. The method for measuring the arithmetic mean roughness Ra of the release surface of the release sheet in this specification is as shown in the test examples described later.

[0078] (2) Manufacturing of analytical cover film As described above, once the adhesive layer 20 is formed on the release surface of the release sheet, the adhesive layer 20 on the release sheet and the base film 10 are bonded together to obtain the analytical cover film 1. The release sheet protects the adhesive layer 20 until the analytical cover film 1 is used, and is peeled off when the analytical cover film 1 is used.

[0079] Here, if the adhesive composition P contains an active energy ray curable component (C), it is preferable to cure the adhesive layer 20 by active energy ray irradiation before attaching the analytical cover film 1 (adhesive layer 20) to the analytical substrate 2. This makes it less likely for the adhesive layer 20 to become embedded in the grooves 4 of the analytical substrate 2, and makes it easier to obtain an adhesive layer 20 with high cohesiveness. When a release sheet with an arithmetic mean roughness Ra of the release surface of 8 nm or less is used, curing the adhesive composition P by active energy ray irradiation after applying the adhesive composition P to the release sheet can fix the smooth shape of the release surface of the release sheet transferred to the adhesive surface of the adhesive layer 20, making it easier to obtain a smooth adhesive surface.

[0080] Active energy rays refer to electromagnetic waves or charged particle beams that possess energy quanta, specifically including ultraviolet rays and electron beams. Among active energy rays, ultraviolet rays are particularly preferred because they are easy to handle.

[0081] Ultraviolet irradiation can be performed using high-pressure mercury lamps, Heraeus H lamps, xenon lamps, etc., with an illuminance of 50-1000 mW / cm². 2 It is preferable that the light intensity be around 50 to 10,000 mJ / cm². 2 Preferably, it is 80-5000 mJ / cm². 2 It is more preferable that the concentration be 300-2000 mJ / cm². 2 It is particularly preferable that this is the case. On the other hand, electron beam irradiation can be performed by an electron beam accelerator or the like, and the electron beam irradiation dose is preferably about 10 to 1000 krad.

[0082] 3. Characteristics of the analytical cover film and adhesive layer (1) Characteristics of the adhesive layer (1-1) Thickness of the adhesive layer The thickness of the adhesive layer 20 is preferably 0.5 μm or more, particularly preferably 0.8 μm or more, and even more preferably 1 μm or more. From the viewpoint of imparting high tackiness to the adhesive layer 20, the thickness of the adhesive layer 20 may be 7 μm or more. This makes it possible to fix the analytical cover film 1 to the analytical substrate 2 well. Furthermore, the above thickness is preferably 30 μm or less, particularly preferably 20 μm or less, and even more preferably 15 μm or less. This reduces the influence of the thickness of the adhesive layer 20 on the analysis, making it easier to perform good analysis on the resulting analytical member 100.

[0083] (1-2) Arithmetic mean roughness Ra The arithmetic mean roughness Ra of the adhesive surface of the adhesive layer 20 is preferably 14 nm or less, more preferably 10 nm or less, particularly preferably 8 nm or less, and even more preferably 4 nm or less. This ensures that the adhesive surface of the adhesive layer 20 is smooth, making it easier to satisfy the haze value of the analytical cover film 1 described above. It also improves adhesion to the analytical substrate 2, making it easier to achieve good adhesive strength. The lower limit of the arithmetic mean roughness Ra is not particularly limited, but is usually preferably 0.5 nm or more, and particularly preferably 1 nm or more. The method for measuring the arithmetic mean roughness Ra of the adhesive surface of the adhesive layer in this specification is as shown in the test examples described later.

[0084] (2) Transmittance of analytical light The transmittance of analytical light in the analytical cover film 1 according to this embodiment is preferably 60% or more, particularly preferably 80% or more, and even more preferably 90% or more. This allows the analytical cover film 1 to have better transmittance to analytical light. As a result, it becomes possible to perform more accurate analysis with the analytical member 100 using the analytical cover film 1. The upper limit of the above transmittance is not particularly limited, and is 100% or less.

[0085] (3) Thickness of the cover film for analysis In this embodiment, the thickness of the analytical cover film 1 is preferably 10 μm or more, particularly preferably 30 μm or more, and even more preferably 75 μm or more. This ensures that the analytical cover film 1 has sufficient strength, is easy to handle, and suppresses deformation and damage to the analytical cover film 1 when using the analytical member 100. When laser light is used for analysis, a thickness of 75 μm or more in the analytical cover film 1 makes it easier to focus on the analyte in the flow path. Furthermore, the above thickness is preferably 300 μm or less, particularly preferably 250 μm or less, and even more preferably 200 μm or less. This makes it easier for the analytical cover film 1 to have excellent transmittance to analytical light, and facilitates good analysis of the resulting analytical member 100. Note that the thickness of the analytical cover film is the thickness excluding the release sheet if a release sheet is laminated to the analytical cover film.

[0086] [Analytical components] Figure 2 shows a cross-sectional view of the analysis member 100 according to this embodiment. The analysis member 100 comprises an analysis substrate 2 and the analysis cover film 1 described above, which is attached to the analysis substrate 2. The analysis substrate 2 has a groove 4 as a housing portion for housing the object to be analyzed, and a planar adhesive attachment portion 3. The analysis cover film 1 is attached to the analysis substrate 2 by the adhesive layer 20 of the analysis cover film 1 being attached to the adhesive attachment portion 3. The groove 4 of the analysis substrate 2 has its opening (the upper surface of the groove 4 in Figure 2) sealed by the analysis cover film 1, thereby forming a flow path. In this analysis member 100, optical analysis is performed on the object to be analyzed housed in the groove 4 (flow path).

[0087] In the analytical member 100 according to this embodiment, the haze value of the analytical cover film 1 is 1% or less. Therefore, when light is irradiated onto the object to be analyzed contained in the groove 4 through the analytical cover film 1, or when light emitted from the object to be analyzed contained in the groove 4 is acquired, light scattering is suppressed, resulting in excellent light transmittance and enabling accurate optical analysis.

[0088] The shape of the analysis member 100 in plan view according to this embodiment is not particularly limited, but it is preferably disc-shaped or chip-shaped. Disc-shaped means that the shape of the analysis member 100 in plan view is a perfect circle or a deformed shape thereof. If it is disc-shaped, a hole may be provided in the center of the analysis member 100, and the shape of the hole may be a circular shape that is concentric with the outer circumference of the analysis member 100 in plan view. Chip-shaped means that the shape of the analysis member 100 in plan view is a square, a rectangle, or a deformed shape thereof.

[0089] 1. Components of the analytical material (1) Cover film for analysis The analytical cover film 1 in this embodiment is the one described in the previously mentioned embodiment.

[0090] (2) Analysis base material In the analytical member 100 according to this embodiment, the analytical substrate 2 is not particularly limited as long as it is possible to perform analysis using the analytical member 100 equipped with the analytical substrate 2. However, from the viewpoint of enabling good analysis, it is preferable that the analytical substrate 2 is transparent to analytical light. The shape of the analytical substrate 2 is preferably plate-shaped, but is not limited thereto.

[0091] The material for the analytical substrate 2 can be resin, glass, etc., but it is preferable to use resin from the viewpoint of ease of manufacture and handling. The resin can be the same as the resin that makes up the resin film that can be used as the base film 10. Among such resins, it is preferable to use polycarbonate, cycloolefin copolymer, polyethylene terephthalate film, or acrylic resin from the viewpoint of excellent transmittance to analytical light and excellent moldability, and in particular, it is preferable to use cycloolefin copolymer or polycarbonate. Furthermore, as mentioned above, from the viewpoint of minimizing the difference in transmittance to analytical light, it is preferable to use the same material for the analytical substrate 2 as the material for the base film 10.

[0092] The transmittance of analytical light in the analytical substrate 2 is preferably 60% or more, particularly preferably 80% or more, and even more preferably 90% or more. A transmittance of 60% or more allows the analytical component 100 to have better transmittance to analytical light, enabling more accurate analysis. The upper limit of the transmittance is not particularly limited, but is 100% or less.

[0093] The haze value of the analytical substrate 2 is preferably 10% or less, particularly preferably 5% or less, and even more preferably 1% or less. A haze value of 10% or less for the analytical substrate 2 effectively reduces the scattering of analytical light in the analytical substrate 2, enabling more accurate analysis in the analytical component 100. While there are no particular limitations on the lower limit of the haze value of the analytical substrate 2, it is usually 0% or higher.

[0094] In this embodiment, it is preferable that the analytical substrate 2 has at least one groove 4 on one side thereof. Here, in the analytical substrate 2 of the analytical member 100 shown in Figure 2, multiple grooves 4 with a width w1 and a depth d1 (three in Figure 2) are provided on the side of the analytical substrate 2 facing the adhesive layer 20.

[0095] The cross-sectional shape of groove 4 is not limited as long as it can accommodate the analyte and allow for good analysis. In Figure 2, the shape of the cross-section is triangular, but it is not limited to this and may be square, rectangular, semicircular, etc.

[0096] The shape of the groove 4 in plan view is not limited as long as it can accommodate the analyte and allow for good analysis; for example, it may be linear, dotted, etc. However, from the viewpoint of allowing the analytical light to be scanned along the groove 4, a linear shape is preferable.

[0097] The various dimensions of the groove 4 can be set according to the analysis method and the type of analyte being analyzed. For example, the width w1 of the groove 4 is preferably 50 nm or more, particularly preferably 100 nm or more, and even more preferably 150 nm or more. Furthermore, the width w1 is preferably 30 μm or less, particularly preferably 10 μm or less, and even more preferably 1 μm or less. The depth d1 of the groove 4 is preferably 50 nm or more, particularly preferably 100 nm or more, and even more preferably 150 nm or more. Furthermore, the depth d1 is preferably 30 μm or less, particularly preferably 10 μm or less, and even more preferably 1 μm or less. By having the width w1 and depth d1 of the groove 4 within the above ranges, it is possible to suppress the amount of analyte required while ensuring a sufficient length for the analytical light to pass through the analyte.

[0098] The thickness of the analytical substrate 2 (the distance between the surface to which the adhesive layer 20 is attached and the opposite surface) is preferably 0.5 mm or more, particularly preferably 0.8 mm or more, and even more preferably 1 mm or more. This ensures that the analytical substrate 2 has sufficient strength, effectively suppressing deformation of the analytical member 100 when containing the analyte and during analysis. Furthermore, the thickness is preferably 10 mm or less, particularly preferably 5 mm or less, and even more preferably 3 mm or less. This allows the analytical light to reach the analyte more easily during analysis, facilitating high-precision analysis.

[0099] The method for manufacturing the analytical substrate 2 is not particularly limited. However, if the analytical substrate 2 is made of resin, it is preferable to manufacture it by molding it by injection molding, compression molding, insert molding, etc., and applying surface treatment as necessary.

[0100] (3) Others The analytical member 100 according to this embodiment may have a hole for receiving the object to be analyzed in the groove 4. The receiving hole may be provided in at least one of the analytical cover film 1 and the analytical substrate 2, but it is particularly preferable to provide it in the analytical cover film 1. The shape and size of the receiving hole are preferably suitable for the receiving means used to house the object to be analyzed in the groove 4. Furthermore, if the analytical member 100 according to this embodiment has the above-mentioned receiving hole, it is also preferable to have an air vent hole.

[0101] The containment section for the analyte has openings on both the front and back of the analysis substrate 2, and may also be a slit that penetrates both sides. In this case, both the front and back surfaces of the analysis substrate 2 function as adhesive application areas 3, and by attaching the analysis cover film 1 to both surfaces, the slit sandwiched between the analysis cover film 1 functions as a flow path.

[0102] 2. Method for manufacturing analytical components To manufacture the analytical member 100 according to this embodiment, the analytical cover film 1 is attached to the analytical substrate 2 such that the adhesive layer 20 of the analytical cover film 1 is attached to the adhesive-bonded portion 3 of the analytical substrate 2. If a release sheet is laminated on the adhesive layer 20 of the analytical cover film 1, the release sheet is peeled off to expose the adhesive layer 20 before the above attachment is performed. In this way, the opening of the housing portion is sealed by the analytical cover film 1, and the analytical member 100 with a flow channel can be manufactured very easily.

[0103] 3. How to use the analytical material The analytical member 100 according to this embodiment can be used for the optical analysis of an analyte. Specifically, after placing the analyte in the flow path of the analytical member 100, light is irradiated onto the placed analyte from outside the analytical member 100, and the resulting light (including transmitted light, scattered light, and fluorescence) is measured outside the analytical member 100.

[0104] The primary material to be analyzed is a specimen, but it is not limited to this. A specimen typically refers to the material used in medical tests, such as blood, cerebrospinal fluid, urine, tissue, or cells, which are excreted or collected from the human body.

[0105] From the viewpoint of easy containment in the flow path, the analyte is preferably a fluid with good flow properties. Examples include liquids, sol-like components, gel-like components, etc., but a liquid is particularly preferred. The liquid may also contain solid components. Specific examples of analytes include water or extracts used in water quality or soil analysis, cell extracts, blood, cultured cell fluid, bacteria, archaea, viruses, proteins, algae, microorganisms, etc.

[0106] The analyte can be introduced into the flow path by, for example, supplying it to the flow path through a receiving hole provided in the analytical member 100 using the aforementioned introduction means. Alternatively, a syringe equipped with a needle may be used to insert the needle into a predetermined position in the analytical member 100, and then supply the analyte to the flow path through the needle.

[0107] The irradiation of the contained analyte with analytical light and subsequent measurements are selected according to the purpose of the analysis. Examples include absorbance measurement, Raman spectroscopy, and fluorescence analysis. The type of analytical light can be selected according to the purpose of the analysis. For example, visible light, ultraviolet light, etc., can be used, or laser light may be used.

[0108] When using the analytical member 100, the analytical member 100 may be cooled or heated depending on the analyte used and the analysis performed. When heating the analytical member 100, it is more preferable to use an adhesive layer formed from an adhesive composition P containing an active energy ray curable component (C) from the viewpoint of suppressing thermal deformation of the adhesive layer 20.

[0109] The embodiments described above are provided to facilitate understanding of the present invention and are not intended to limit it. Accordingly, each element disclosed in the above embodiments is intended to include all design modifications and equivalents that fall within the technical scope of the present invention.

[0110] For example, the adhesive layer 20 of the analytical cover film 1 may be laminated only on a portion of one side of the base film 10, as long as it is possible to bond it to the analytical substrate 2. [Examples]

[0111] The present invention will be described in more detail below with reference to examples, but the scope of the present invention is not limited to these examples.

[0112] [Preparation Example 1] (Preparation of coating solution (P1) for adhesive composition) A (meth)acrylic acid ester polymer was prepared by copolymerizing 95 parts by mass of n-butyl acrylate and 5 parts by mass of acrylic acid using a solution polymerization method. The molecular weight of this acrylic acid ester copolymer was measured by the method described later, and the weight-average molecular weight (Mw) was found to be 2 million.

[0113] 100 parts by mass (on a solid content basis; the same applies hereinafter) of the above (meth)acrylic acid ester polymer, 3 parts by mass of trimethylolpropane-modified tolylene diisocyanate as a crosslinking agent, 20 parts by mass of a mixture of ethylene oxide-modified isocyanuric acid diacrylate and ethylene oxide-modified isocyanuric acid tri(meth)acrylate (manufactured by Toagosei Co., Ltd., product name "Aronics M315") as active energy ray curable components, 1.5 parts by mass of a photoradical polymerization initiator (manufactured by IGM Regin, product name "Omnirad 500"), and 1 part by mass of 3-glycidoxypropyltrimethoxysilane as a silane coupling agent were mixed, stirred thoroughly, and diluted with methyl ethyl ketone to obtain a coating solution (P1) of the adhesive composition.

[0114] [Preparation Example 2] (Preparation of coating solution (P2) for adhesive composition) A (meth)acrylic acid ester polymer was prepared by copolymerizing 95.8 parts by mass of n-butyl acrylate, 4 parts by mass of acrylic acid, and 0.2 parts by mass of 2-hydroxyethyl acrylate using a solution polymerization method. The molecular weight of this acrylic acid ester copolymer was measured by the method described later, and the weight-average molecular weight (Mw) was found to be 1 million.

[0115] 100 parts by mass of the above (meth)acrylic acid ester polymer, 0.2 parts by mass of trimethylolpropane-modified tolylene diisocyanate as a crosslinking agent (isocyanate type), 0.02 parts by mass of 1,3-bis(N,N-diglycidylaminomethyl)cyclohexane as a crosslinking agent (epoxy type), and 0.1 parts by mass of 3-glycidoxypropyltrimethoxysilane as a silane coupling agent were mixed, thoroughly stirred, and diluted with methyl ethyl ketone to obtain a coating solution (P2) of the adhesive composition.

[0116] Here, the weight-average molecular weight (Mw) mentioned above is the weight-average molecular weight in polystyrene terms, measured using gel permeation chromatography (GPC) under the following conditions (GPC measurement). <Measurement conditions> • GPC measuring device: Tosoh Corporation, HLC-8020 • GPC column (passes through in the following order): Manufactured by Tosoh Corporation TSK Guard Column HXL-H TSK gel GMHXL (x2) TSK gel G2000HXL • Measurement solvent: tetrahydrofuran ·Measurement temperature: 40℃

[0117] [Example 1 of release sheet manufacturing] 100 parts by mass of thermosetting addition-reaction type silicone resin (manufactured by Shin-Etsu Chemical Co., Ltd., product name "KS-847H") was diluted with toluene, and then 2 parts by mass of platinum catalyst (manufactured by Shin-Etsu Chemical Co., Ltd., product name "CAT-PL-50T") were added and mixed to obtain a release agent composition coating solution with a solid content concentration of 1.5% by mass. The obtained coating solution was applied to the first surface (hereinafter referred to as the "first surface") of a 25 μm thick polyethylene terephthalate (PET) film having an arithmetic mean roughness Ra of 2.4 nm using a bar coater. The resulting coating film was heated at 125°C for 30 seconds to dry and cure, forming a release agent layer. This obtained a release sheet R1 on the first surface of the PET film. The arithmetic mean roughness Ra of the release surface of the release sheet R1 was 2.7 nm.

[0118] [Example 2 of release sheet manufacturing] Release sheet R4 was obtained in the same manner as in release sheet manufacturing example 1, except that the PET film was replaced with a 25 μm thick PET film having a first surface with an arithmetic mean roughness Ra of 13.3 nm. The arithmetic mean roughness Ra of the release surface of release sheet R4 was 15.6 nm.

[0119] [Example 1] On the release surface of the release sheet R1 obtained in the above manufacturing example, the adhesive composition coating solution (P1) prepared in Preparation Example 1 was applied using a knife coater, and the resulting coating film was dried in a 100°C oven for 1 minute to form an adhesive layer with a thickness of 10 μm.

[0120] Next, the exposed side of the adhesive layer on the release sheet was laminated to one side of a polyethylene terephthalate film (S1; manufactured by Toyobo Co., Ltd., product name "Cosmoshine A4360", thickness: 100 μm) which served as the base film.

[0121] Subsequently, the adhesive layer was irradiated with active energy rays (ultraviolet light; UV) from the side facing the release sheet to cure the adhesive layer. The irradiation conditions for the active energy rays were as follows:

[0122] <Activated energy ray irradiation conditions> • Irradiation device: iGraphics Co., Ltd. "iGrantage ECS-401GX model" • Light source: High-pressure mercury lamp • Lamp output: 2kW • Conveyor speed: 4.23 m / min ·Illuminance: 240mW / cm 2 ,Light amount:307mJ / cm 2

[0123] Finally, the film was cured for one week at 23°C and 50% relative humidity to obtain an analytical cover film with a release sheet, in which the base film, adhesive layer (10 μm thick), and release sheet were laminated in that order.

[0124] [Example 2] A release sheet (R2; manufactured by Lintec Corporation, product name "SP-PLZ38 3030", arithmetic mean roughness Ra: 10.8 nm of the release surface) was formed by applying a silicone-based release agent layer to one side of a 38 μm thick polyethylene terephthalate film using a knife coater. The resulting coating film was dried in a 100°C oven for 1 minute to form a 5 μm thick adhesive layer.

[0125] Next, the exposed side of the adhesive layer on the release sheet was laminated to one side of a cycloolefin copolymer film (S2; manufactured by Zeon Corporation, product name "Zeonor ZF16-100", thickness: 100 μm) which served as the base film.

[0126] Subsequently, the film was cured for one week at 23°C and 50% relative humidity to obtain an analytical cover film with a release sheet, in which the base film, adhesive layer (5 μm thick), and release sheet were laminated in that order.

[0127] [Example 3] An analytical cover film with a release sheet was manufactured in the same manner as in Example 2, except that the release sheet R1 obtained in the above manufacturing example was used.

[0128] [Comparative Example 1] An analytical cover film with a release sheet was manufactured in the same manner as in Example 1, except that a release sheet (R3; manufactured by Lintec Corporation, arithmetic mean roughness Ra: 23.6 nm of the release surface) was used, which consisted of a 38 μm thick polyethylene terephthalate film with a silicone-based release agent layer formed on one side.

[0129] [Comparative Example 2] An analytical cover film with a release sheet was manufactured in the same manner as in Example 2, except that the release sheet R4 obtained in the above manufacturing example was used, and polyethylene terephthalate film (S1; manufactured by Toyobo Co., Ltd., product name "Cosmoshine A4360", thickness: 100 μm) was used as the base film.

[0130] [Comparative Example 3] An analytical cover film with a release sheet was manufactured in the same manner as in Example 1, except that a release sheet (R2; manufactured by Lintec Corporation, product name "SP-PLZ38 3030", arithmetic mean roughness Ra: 10.8 nm of the release surface) was used, which consisted of a 38 μm thick polyethylene terephthalate film with a silicone-based release agent layer formed on one side.

[0131] [Test Example 1] (Measurement of surface roughness of release sheet and adhesive layer) The arithmetic mean roughness Ra (nm) was measured on the first surface of the PET film used in the manufacture of the release sheet, the surface (release surface) of the release sheet used in the examples and comparative examples, and the surface (adhesive surface) of the exposed adhesive layer after peeling off the release sheet from the analytical cover film with the release sheet manufactured in the examples and comparative examples. For this measurement, an optical interference surface shape observation device (Veeco, product name "WYKO-1100") was used, and a 91.2 μm × 119.8 μm area of ​​the surface of the release sheet or adhesive layer was measured in PSI mode at 10x magnification. The results are shown in Table 1. The results for the arithmetic mean roughness Ra of the first surface of the PET film used in the manufacture of the release sheet and the release surface of the release sheet are as described above.

[0132] [Test Example 2] (Measurement of haze value) The release sheets were peeled off from the analytical cover films with release sheets manufactured in the examples and comparative examples, and the haze value (%) of the obtained inspection cover films was measured using a haze meter (manufactured by Nippon Denshoku Industries Co., Ltd., product name "NDH-5000") in accordance with JIS K7136:2000. The results are shown in Table 1.

[0133] [Table 1]

[0134] As is clear from Table 1, it was found that the inspection cover film manufactured in the examples allows for good optical analysis. [Industrial applicability]

[0135] The analytical cover film, analytical member, and analytical method according to the present invention are suitable for the optical measurement of trace amounts of analytes. [Explanation of symbols]

[0136] 1…Cover film for analysis 10…Base film 20…Adhesive layer 100…Analytical component 2…Analysis base material 3… Adhesive application area 4… Groove (storage area)

Claims

1. An analytical cover film to be attached to an analytical substrate having a containment section for containing the object to be analyzed and an adhesive application section, The system comprises a base film and an adhesive layer made of a pressure-sensitive adhesive laminated on one side of the base film. The adhesive layer is laminated on the base film so as to face the accommodating portion and the adhesive application portion of the analytical substrate. The haze value measured by the haze meter is 1% or less. An analytical cover film characterized by the following features.

2. The analytical cover film according to claim 1, characterized in that the arithmetic mean roughness Ra of the side of the adhesive layer that is attached to the analytical substrate is 14 nm or less.

3. The analytical cover film according to claim 1, characterized in that a release sheet is laminated on the adhesive layer, and the arithmetic mean roughness Ra of the surface of the release sheet in contact with the adhesive layer is 15 nm or less.

4. The analytical cover film according to claim 1, characterized in that the base film contains one or more resins selected from the group consisting of cycloolefin copolymers and polycarbonates.

5. The analytical cover film according to claim 1, characterized in that a hard coat layer is formed on the surface of the base film opposite to the adhesive layer.

6. An analytical substrate having a containment section for containing the object to be analyzed and an adhesive application section, The analytical cover film according to any one of claims 1 to 5, which is attached to the analytical substrate, Equipped with, An analytical member in which a flow path is formed by sealing the opening of the housing portion with the analytical cover film.

7. Prepare the analytical member described in claim 6, The fluid containing the object to be analyzed is contained in the flow path of the analytical member. An analytical method for analyzing an object to be analyzed using light transmitted through the analytical cover film.

Images