Peptide production method

Abstract

The present invention aims to provide a method for efficiently producing specific peptides from whey protein-containing materials. In particular, it aims to provide materials with a high GTWY content, a low WY content, and good flavor, as well as food products using these materials. [Solution] When hydrolyzing whey protein with a protease derived from Aspergillus meleus, we found that maintaining the pH of the reaction solution on the alkaline side and performing the enzymatic reaction for a relatively short time not only promotes the cleavage of GTWY from whey protein but also suppresses the degradation of GTWY by the protease, making it possible to produce a flavorful material with a high GTWY content.

Claims

1. A method for producing a GTWY-containing composition by reacting a whey protein-containing material with a protease derived from Aspergillus meleus, wherein the GTWY-containing composition has an average molecular weight of 500 or more, a molar ratio of GTWY to WY of 2.0 or more, a pH of the reaction solution at the start of the reaction in which the protease is reacted is 8.5 or more, and a reaction time of 10 minutes or more and less than 4 hours.

2. The method for producing whey protein according to claim 1, wherein the whey protein is β-lactoglobulin.

3. A method for producing a GTWY-containing composition by reacting a whey protein-containing material with a protease derived from Aspergillus meleus, wherein the GTWY-containing composition has an average molecular weight of 500 or more, a molar ratio of GTWY to WY of 2.0 or more, the pH of the reaction solution in which the protease is reacted is maintained at 8.1 or more and 8.9 or less, and the reaction time is 10 minutes or more and less than 4 hours.

4. The method for producing whey protein according to claim 3, wherein the whey protein is β-lactoglobulin.

5. A method for inhibiting the degradation of GTWY in a method for producing a GTWY-containing composition by reacting a whey protein-containing material with a protease derived from Aspergillus meleus, characterized in that the degradation of GTWY is inhibited by setting the pH of the reaction solution to 8.5 or higher at the start of the reaction in which the protease is reacted, and setting the reaction time to 10 minutes or more and less than 4 hours.

Images