Animal oral bacteria reducing agent, animal oral bacteria reducing toothpaste, animal oral bacteria reducing mouthwash, animal oral bacteria reducing gum, animal oral bacteria reducing gel, and method for reducing animal oral bacteria

Abstract

The main objective is to provide an oral bacteria reducing agent for animals that contains active ingredients that do not pose problems even when absorbed into the body via the oral cavity over the long term, and that can reduce the number of bacteria that cause multiple types of periodontal disease. [Solution] The present invention provides an oral fungal inhibitor for animals, comprising seaweed powder as an active ingredient, wherein the seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of seaweed, and contains 0.01 mass% or more of iodine, and is characterized by reducing the number of oral fungi by spreading the oral fungal inhibitor throughout the oral cavity of an animal.

Description

Technical Field

[0001] The present invention relates to an oral fungal reducing agent for animals, an oral fungal reducing toothpaste for animals, an oral fungal reducing mouthwash for animals, an oral fungal reducing gum for animals, an oral fungal reducing gel for animals, and a method for reducing oral fungi in animals.

Background Art

[0002] Similar to humans, it is known that hundreds of types of bacteria inhabit the oral cavities of animals including dogs and cats. Examples of causative bacteria of periodontal disease in animals include Porphyromonas denticanis (P. denticanis bacterium), Porphyromonas salivosa (P. salivosa bacterium), Treponema denticola (T. denticola bacterium), Porphyromonas gulae (P. gulae bacterium), etc. Periodontal disease is a chronic disease in which the causative bacteria of periodontal disease grow in plaque adhering to the periodontal pocket, which is the groove between the gum and the tooth, and discharge toxins, resulting in the destruction of the periodontal pocket and the deepening of the pocket.

[0003] By the way, in recent years, toothpastes (tooth powders, liquid toothpastes, etc.), mouthwashes (dental rinses, mouthwashes, etc.), gums, gels, foods, etc. that have the effect of removing the causative bacteria of periodontal disease in the oral cavity of animals and preventing periodontal disease have been sold. However, toothpastes and mouthwashes often contain, for example, 1,3,3-trimethyl(1,8-cineole), thymol, methyl salicylate, cetylpyridinium chloride, etc., which are originally disinfectants and suppress the growth of the causative bacteria that cause periodontal disease, as bactericides, and in addition, dipotassium glycyrrhizinate, etc., which have a sedative effect on gum and oral inflammation, are blended. There are also some that contain antibiotics that have an effect of suppressing the growth of some bacteria. Furthermore, in order to efficiently remove dental plaque and tooth discoloration even for animals that dislike brushing their teeth, it is also necessary to set a high concentration of the active ingredient. These disinfectants, antibiotics, and other components are not something that animals would normally ingest on a daily basis. They are absorbed into the body via the oral cavity, which poses a problem in the long term.

[0004] Furthermore, even if the components of the antibacterial agents used to kill bacteria that cause periodontal disease are natural products, they are not typically consumed, or the antibacterial components are not water-soluble and therefore need to be dissolved in a solvent that is a non-natural product (artificially synthesized), and in addition, artificial sweeteners are sometimes used in combination with antibacterial agents as auxiliary agents (see Patent Document 1, etc.). Even natural disinfectants (antimicrobial agents) that are insoluble or poorly soluble in water require the addition of non-natural solvents and additives during product development, which can lead to direct or indirect absorption into the body, posing a problem in the long term.

[0005] Furthermore, Patent Document 2 discloses that a seaweed extract has specific antibacterial activity only against Porphyromonas gingivalis (Pg bacteria), which is known to be a causative agent of periodontal disease in humans. However, since this antibacterial activity is expressed in terms of the minimum inhibitory concentration (MIC, ppm), there was a problem that the seaweed extract in Patent Document 2 inhibits growth but does not reduce the number of bacteria. In addition, although it has been shown that the antibacterial activity of chloroform-methanol extract is higher than that of water extract regarding Porphyromonas gingivalis (Pg bacteria), only Fusobacterium nucleatum (Fn bacteria) is disclosed as a causative agent of other periodontal disease bacteria, and moreover, the antibacterial activity of Fusobacterium nucleatum (Fn bacteria) has not been confirmed. In other words, there was a problem that the seaweed extract in Patent Document 2 does not have antibacterial activity against multiple causative agents of periodontal disease. Furthermore, since seaweed extracts require the removal of extraction residue during the extraction process, there was a challenge in that it was not possible to manufacture oral bacteria reducing agents that fully utilized the components of the seaweed before extraction.

[0006] Patent Document 3 shows that an oral composition for animals containing mastic resin and / or mastic essential oil as an active ingredient is effective in reducing Porphyromonas gulae (P. gulae bacteria) in the oral cavity of animals. However, similar to Patent Document 1, there were problems such as the need to dissolve the antibacterial component in a solvent that is a non-natural product (artificially synthesized product) because it is not water-soluble. [Prior art documents] [Patent Documents]

[0007] [Patent Document 1] Japanese Patent Publication No. 2020-620006 [Patent Document 2] Japanese Patent Publication No. H9-48715 [Patent Document 3] Japanese Patent Publication No. 2017-75098 [Overview of the project] [Problems that the invention aims to solve]

[0008] The main objective of this invention is to provide an oral bacteria reducing agent for animals that contains an active ingredient that does not pose a problem even if absorbed into the body via the oral cavity of an animal over a long period of time, and that can reduce the number of one or more bacteria that cause periodontal disease. [Means for solving the problem]

[0009] In view of the above circumstances, the inventors conducted diligent research and found an oral bacteria reducing agent for animals that contains an active ingredient that does not cause problems even if absorbed into the body via the oral cavity over a long period of time, and that can reduce the number of one or more bacteria that cause periodontal disease.

[0010] In other words, the present invention is an animal oral fungal reducing agent that contains seaweed powder as an active ingredient, wherein the seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of seaweed, and contains 0.01 mass% or more of iodine, and is characterized by reducing the number of oral fungi by spreading the animal oral fungal reducing agent throughout the oral cavity of the animal.

[0011] The oral bacteria of the animal may be selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, Treponema denticola, and Porphyromonas gulae, with one or more species being chosen.

[0012] The oral bacteria of the animal may be selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, and Treponema denticola, and the number of the selected oral bacteria may be reduced simultaneously.

[0013] The aforementioned seaweed powder preferably has a pH of 5 to 8.

[0014] The present invention relates to an animal oral bacteria reducing toothpaste, characterized in that the above-mentioned animal oral bacteria reducing agent is incorporated as an active ingredient in toothpaste or liquid toothpaste.

[0015] The present invention is characterized by comprising the above-mentioned animal oral fungus reducing agent as an active ingredient in a mouthwash solution.

[0016] The present invention provides a gum for reducing oral bacteria in animals, characterized in that the above-mentioned oral bacteria-reducing agent for animals is incorporated into the gum as an active ingredient.

[0017] The oral fungal reduction gel for animals of the present invention is characterized in that the above-mentioned oral fungal reducing agent for animals is formulated into a gel as an active ingredient.

[0018] The method for reducing oral fungi in animals of the present invention includes a step of spreading the above-mentioned oral fungal reducing agent for animals in the oral cavity of an animal, and a step of repeating the step of spreading in the oral cavity for a certain period.

[0019] The step of spreading in the oral cavity may include a step of spreading 0.8 g or more of the oral fungal reducing agent for animals in the oral cavity per day.

Effect of the Invention

[0020] According to the present invention, it is possible to provide an oral fungal reducing agent for animals, in which the active ingredient is not a problem even if it is absorbed into the animal's body through the oral cavity for a long time, and the number of bacteria of one or more causative bacteria of periodontal disease is reduced.

Embodiment for Carrying Out the Invention

[0021] Embodiments of the present invention will be specifically described below.

[0022] (Oral fungal reducing agent for animals) The oral fungal reducing agent for animals of the present invention contains a powder of seaweeds as an active ingredient. An oral fungal reducing agent for animals is an agent having an effect of reducing the number of bacteria in the oral cavity of animals, particularly the number of fungi distributed in periodontal pockets. The reduction in the number of fungi is mainly considered to be due to the effect of sterilization, but the contribution of the antibacterial effect of preventing the growth of bacteria is not denied. In addition, the "animal" in this specification is an animal that needs to reduce periodontal bacteria, and examples include dogs, cats, monkeys, etc.

[0023] Veterinary oral bacteria reducing agents can reduce the total number of bacteria in the oral cavity of animals. In particular, they can reduce Porphyromonas denticanis, Porphyromonas salivosa, Treponema denticola, and Porphyromonas gulae, which are causative agents of periodontal disease in animals.

[0024] (Seaweed powder) Seaweed powder is a powder obtained by drying and grinding seaweed, or a powder obtained by drying seaweed extract.

[0025] When drying and grinding seaweed to obtain a powder, theoretically, all components of the seaweed except water can be contained in the powder. Here, the drying temperature is preferably a temperature similar to those used for drying food (for example, around 60°C). The drying time should be adjusted as follows: if you want to reduce the residual moisture content to near zero, continue drying until there is almost no weight loss in the dried material; if you want to retain some moisture, shorten the drying time. The grinding time should be adjusted according to the particle size of the resulting powder.

[0026] When extracting seaweed and drying the extract to obtain a powder, the extraction residue is not contained in the powder. The composition of the resulting powder varies depending on the extraction solvent, extraction temperature, extraction time, etc. In particular, the components that are soluble and dissolve in the extract differ depending on the selection of the extraction solvent. Examples of extraction solvents include water, alcohol, organic solvents, and mixtures thereof. Furthermore, a drying and grinding step may be included before the extraction step to improve extraction efficiency.

[0027] The seaweed powder contains a certain amount or more of iodine as a component of the seaweed powder. The iodine content is 0.01 mass% or more, preferably 0.1 mass% or more, and more preferably 0.2 mass% or more. Examples of seaweeds that contain a certain amount or more of iodine include kelp, hijiki, and wakame. In order to ensure that the seaweed powder contains a certain amount or more of iodine, it is preferable to select seaweed with a high iodine content and to dry and grind the seaweed to obtain the powder so that the iodine is included in the powder. Even when drying an extract of seaweed to make a powder, it is acceptable as long as the extraction conditions allow for the specific extraction of iodine.

[0028] The pH of the seaweed powder is preferably between 5 and 8, and more preferably between 5.5 and 7. The pH of the seaweed powder is measured by dissolving a certain amount in water. Since it is for repeated oral use, if the pH is acidic (below 5), there is a higher possibility of damaging the oral mucosa or causing tooth erosion. Similarly, if the pH is alkaline (above 8), it is more likely to damage the oral mucosa.

[0029] Based on the pH range of the seaweed powder, it is thought that the iodine contained in the seaweed powder does not take the structure of a polyvinylpyrrolidone-iodine complex, which is known as povidone-iodine (Isodine), known for its high bactericidal properties. The pH of povidone-iodine is known to be around 1.5 to 3.5 in a 1% aqueous solution.

[0030] The particle size of the seaweed powder is set appropriately to ensure it can spread throughout the oral cavity, but examples include sizes of 5 μm to 50 μm. Since seaweed powder contains components that do not dissolve easily in water, a finer particle size is preferable for spreading throughout the periodontal pockets and other areas in the oral cavity.

[0031] (oral fungi) It is believed that there are several hundred types of oral bacteria in animals. Oral bacteria may include all oral bacteria. In this invention, "reducing the number of oral bacteria" means reducing the total number of oral bacteria. Furthermore, one or more species may be selected as oral bacteria from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, Treponema denticola, and Porphyromonas gulae. Alternatively, all of the following species may be selected as oral bacteria: Porphyromonas denticanis, Porphyromonas salivosa, and Treponema denticola. All of these are bacteria that cause periodontal disease in animals, including dogs and cats.

[0032] There are various methods for diagnosing periodontal disease, but one example is evaluation by observing the condition of the oral cavity. Evaluation can be performed using a score of 0, where "there is no inflammation of the gums and healthy gums are observed. There is no plaque." A score of 1 (mild periodontal disease) is given for "mild redness and swelling of the gingivitis. Plaque is attached to less than 1 / 3 of the tooth surface." A score of 2 (moderate periodontal disease) is given for "the gums bleed when a probe is inserted. Plaque is attached to less than 2 / 3 of the tooth surface." A score of 3 (severe periodontal disease) is given for "the gums bleed spontaneously. Plaque is attached to more than 2 / 3 of the tooth surface."

[0033] Furthermore, there are various methods for examining bacteria in the oral cavity. One example is to collect plaque from the interdental spaces of designated locations using animal interdental paper points, suspend it in 1 mL of sterile phosphate buffer, freeze and store it at -15°C or below, and quantify the amount of DNA of each oral bacterium by real-time PCR.

[0034] The total number of oral bacteria in animals can be measured, for example, by collecting plaque from the interdental spaces of a designated location using an animal interdental paper point, suspending it in 1 mL of sterile phosphate buffer, storing it frozen at -15°C or below, and performing quantitative analysis using real-time PCR with the protein elongation factor Tu(tuf) gene.

[0035] (Application to the oral cavity) Veterinary oral fungal inhibitors should be applied so that they spread throughout the oral cavity. For example, one method of application is to take a veterinary oral bacteria reducing agent suspended in sterile water for injection onto a gloved index finger and rub it directly into the interdental spaces of the animal. Alternatively, the powder of the veterinary oral bacteria reducing agent may be rubbed directly into the interdental spaces of the animal. Furthermore, teeth may be brushed with toothpaste or liquid toothpaste containing an animal oral bacteria reducing agent as an active ingredient. Or, the mouth may be rinsed with a mouthwash containing an animal oral bacteria reducing agent as an active ingredient. Or, gum containing an animal oral bacteria reducing agent as an active ingredient may be chewed, or it may be incorporated into a gel and applied topically, or if it consists only of edible ingredients, it may be mixed into food.

[0036] (Other ingredients) While animal oral bacteria reducing agents use seaweed powder as their active ingredient, the effectiveness in reducing oral bacteria in animals can vary due to individual differences, regional differences, seasonal differences, powder processing conditions, and even the conditions under which the animal oral bacteria reducing agent is distributed within the oral cavity. Therefore, it is possible to include auxiliary agents that have an oral bacteria reducing effect on their own in the seaweed powder as needed.

[0037] The oral fungal inhibitor for animals may further contain iodine powder and / or zinc powder as auxiliary agents. Unlike povidone-iodine, iodine powder has a pH of approximately 4.5 and exhibits a high bacterial reduction effect against periodontal disease bacteria. Therefore, even a small amount of iodine powder can function as an additive, and the amount can be, for example, between 0.01% and 100% by mass relative to the mass of the seaweed powder, or between 0.01% and 50% by mass. Iodine powder itself tends to reduce periodontal disease bacteria in a short time (for example, within 1 minute), making it suitable for addition as an additive. Since zinc powder has been shown to have a fungal-reducing effect against periodontal disease bacteria, it is thought to function as an auxiliary agent even when added in small amounts. The amount of zinc powder added may be, for example, 0.1% by mass or more and less than 40% by mass relative to the mass of seaweed powder.

[0038] As an auxiliary agent, it may further contain one or more selected from the group consisting of phloroglucinol powder, black grape skin powder, and citric acid powder. Furthermore, it may contain laminaran powder, ascophyllum nodosum powder, ascoraffin powder, β-carotene powder, seaweed micron powder, green tea, etc.

[0039] (Toothpaste for reducing oral bacteria in animals) Animal oral bacteria reducing toothpaste is a toothpaste or liquid toothpaste that contains an animal oral bacteria reducing agent as an active ingredient.

[0040] (Oral bacteria-reducing mouthwash for animals) The mouthwash for reducing oral bacteria in animals is a mouthwash solution that contains an oral bacteria-reducing agent for animals as its active ingredient. The animal oral bacteria reducing agent is formulated in an appropriate amount so that it can be distributed throughout the oral cavity when used for rinsing with the animal oral bacteria reducing mouthwash.

[0041] (Gum for reducing oral bacteria in animals) Animal oral bacteria reducing gum is a gum that contains an animal oral bacteria reducing agent as its active ingredient. The animal oral bacteria reducing agent is formulated in an appropriate amount so that it spreads throughout the oral cavity when the animal chews the oral bacteria reducing gum. It is preferable to adjust the amounts of the active ingredient and other components, taking into consideration that animals, like humans, swallow the gum without chewing it for an extended period.

[0042] (Gel to reduce oral bacteria in animals) Animal oral bacteria reducing gel is a gel containing an animal oral bacteria reducing agent as its active ingredient. The animal oral bacteria reducing agent is appropriately formulated so that it spreads throughout the oral cavity, either by applying the animal oral bacteria reducing gel to the teeth or by making the gel into a food product.

[0043] (Methods for reducing oral bacteria in animals) A method for reducing oral bacteria in animals includes the steps of distributing an oral bacteria reducing agent for animals throughout the animal's oral cavity, and repeating the distributing step for a certain period of time.

[0044] The process of distributing the oral bacteria reducing agent throughout the oral cavity can be exemplified by, for example, taking the animal oral bacteria reducing agent suspended in sterile water for injection onto a gloved index finger and applying it directly to the interdental spaces of the animal. Alternatively, the powder of the animal oral bacteria reducing agent may be applied directly to the interdental spaces of the animal. Furthermore, teeth may be brushed with toothpaste or liquid toothpaste containing an animal oral bacteria reducing agent as an active ingredient. Or, the mouth may be rinsed with a mouthwash containing an animal oral bacteria reducing agent as an active ingredient. Or, gum containing an animal oral bacteria reducing agent as an active ingredient may be chewed, or it may be incorporated into a gel and applied topically, or if it consists only of edible ingredients, it may be mixed into food. The amount of oral bacteria reducing agent used for animals should be adjusted as appropriate depending on factors such as tooth brushing time and frequency, mouth rinsing time and frequency, gum chewing time and frequency, and the amount consumed as food. However, it is desirable to use at least 0.8g per day to ensure it reaches the animal's oral cavity, and even more desirable to use at least 1.0g.

[0045] The process of spreading the substance throughout the animal's mouth, as described above, is repeated for a certain period of time, for example, 3 months to 1.5 years, or continuously thereafter, preferably daily, and more preferably after every meal, by brushing teeth, rinsing mouth, chewing gum, etc.

[0046] (Evaluation Test 1 - Evaluation of reduction in the total number of oral bacteria -) Sample A was prepared as an oral fungal inhibitor for animals by adding 2 mL of purified water to 0.8 g of kelp powder and mixing. Since 0.8 g of kelp powder is used for one dose per dog, the required number of doses was prepared in one batch.

[0047] Six dogs (Beagles) aged 6 months or older were prepared as animals for evaluation. These consisted of three dogs (two males and one female) in the treatment group (receiving sample A) and three dogs (two males and one female) in the control group (not receiving sample A). All dogs were clinical subjects, and their general condition was observed for 7 days prior to the evaluation test to confirm their health. During the acclimatization period, dogs showing no abnormalities were selected and assigned to the treatment group or control group using stratified randomization based on their weight immediately before the start of the evaluation test.

[0048] In a closed-type barn, each animal was housed in a cage measuring W70.5 × D118.5 × H88 cm. A feeder and water dispenser were placed inside the cage. The animals were given 300g of DS-A (Oriental Yeast Co., Ltd.) once a day as feed. They were also given tap water to drink freely. During the evaluation test, the room temperature was kept within the range of 18-29°C. The lighting period consisted of 12 hours of light and 12 hours of dark.

[0049] The treatment group received sample A once daily for three months. The suspended sample A was taken onto a gloved index finger and applied directly to the upper gums and teeth of the dogs by rubbing it in. The control group received no treatment.

[0050] During the 3-month administration period, all dogs in both the treatment and control groups were clinically observed once daily for general conditions such as energy levels, appetite, and fecal characteristics. The results of the clinical observations are shown in Table 1.

[0051] [Table 1]

[0052] As shown in Table 1, no clinical observational abnormalities were observed in either the treatment group or the control group.

[0053] During the 3-month administration period, the oral condition of all dogs in both the treatment and control groups was observed. Gum condition was evaluated according to the scores in Table 2. However, strictly speaking, gum condition varies depending on the position of the teeth, etc., and even with the same overall score, there may be cases where the scores are high / low and cases where they are not very varied. Also, there are four levels of scoring, and there are conditions such as the boundary area between score 0 and 1, and the boundary area between score 1 and 2. Therefore, the area of ​​plaque can also be used as a reference indicator.

[0054] [Table 2]

[0055] During the 3-month administration period, the condition of the oral cavity and surrounding gums (edema, bleeding, discoloration, redness, etc.) was checked after each administration. The results are shown in Table 3.

[0056] [Table 3]

[0057] Table 3 shows that in the control group, scores of 1-2 remained consistent from the start to the end of the evaluation test. In contrast, in the treatment group, dogs that showed a score of 1 at the start of the evaluation study turned to a score of 0 within two weeks, and dogs that showed a score of 2 at the start of the evaluation study maintained a score of 2, but a decrease in plaque area was observed.

[0058] To examine the number of oral bacteria, plaque was collected from the interdental spaces between the upper left premolar and incisor at 0, 0.5, 1, 2, and 3 months from the start of the evaluation test using an animal interdental paper point (Absorbent PaperPoint, TIANJIN GAPADENT), suspended in 1 mL of sterile phosphate buffer, and stored frozen at -15°C or below.

[0059] The above plaque suspension was quantitatively measured using real-time PCR with the protein elongation factor Tu(tuf) gene to confirm the total number of oral bacteria. Table 4 shows the total number of bacteria as the amount of DNA per 1 mL of extracted DNA solution (copies / mL).

[0060] [Table 4]

[0061] Table 3 shows that in the control group, although there were fluctuations in the values ​​from the start to the end of the evaluation test, the overall value was 10. 8 This indicated a copy / mL ratio of approximately [number] copies / mL. In contrast, the treatment group had the same 10 levels as the control group at the start of the evaluation study. 8 The reading was initially copies / mL, but gradually decreased, reaching 10 copies / mL 1 to 3 months after the start of the evaluation study. 6 The result was copies / mL, which was two orders of magnitude lower than the control group. Measurements of the total number of oral bacteria revealed that the treatment group showed a significant decrease in the total bacterial count compared to the control group.

[0062] (Evaluation Test 2 - Evaluation of reduction of Porphyromonas denticanis bacteria -) The plaque suspension obtained in the above evaluation test 1 was quantitatively measured by real-time PCR to determine the number of Porphyromonas denticanis bacteria. Table 5 shows the number of Porphyromonas denticanis bacteria as DNA amount (ng / μL) per 1 μL of extracted DNA solution. In the calculation of the average value, values ​​below the detection limit (<4) were treated as 0.

[0063] [Table 5]

[0064] Table 5 shows that at the start of the evaluation study, Porphyromonas denticanis was detected in all animals in both the treatment group and the control group. In the control group, while values ​​fluctuated during the evaluation test, no significant decrease was observed. In contrast, in the treatment group, Porphyromonas denticanis bacteria were below the detection limit (not detected) in all animals from 0.5 months after the start of the evaluation study.

[0065] (Evaluation Test 3 - Evaluation of reduction of Porphyromonas salivosa bacteria -) The plaque suspension obtained in the above evaluation test 1 was quantitatively measured by real-time PCR to determine the number of Porphyromonas salivosa bacteria. Table 6 shows the number of Porphyromonas salivosa bacteria as DNA amount (ng / μL) per 1 μL of extracted DNA solution. In the calculation of the average value, values ​​below the detection limit (<4) were treated as 0.

[0066] [Table 6]

[0067] As shown in Table 6, at the start of the evaluation study, Porphyromonas salivosa was detected in all animals in both the treatment group and the control group. In the control group, while values ​​fluctuated during the evaluation test, no significant decrease was observed. In contrast, in the treatment group, Porphyromonas salivosa levels were below the detection limit (not detected) in all animals from two months after the start of the evaluation study.

[0068] (Evaluation Test 4 - Evaluation of the reduction of Treponema denticola bacteria -) The plaque suspension obtained in the above evaluation test 1 was quantitatively measured by real-time PCR to determine the number of Treponema denticola bacteria. Table 7 shows the number of Treponema denticola bacteria as DNA amount per 1 mL of extracted DNA solution (copies / mL). Note that the average value was calculated using <10. 3 The calculation was performed using 1000 as the value.

[0069] [Table 7]

[0070] Table 7 shows that in the control group, the period from the start of the evaluation test to the end of the evaluation test was 10 4 Copy / mL ~ 10 5 This indicated a copy / mL ratio of approximately [number] copies / mL. In contrast, in the treatment group, from two months after the start of the evaluation study, all animals showed levels of Treponema denticola (T. denticola) below the detection limit (not detected).

[0071] (Evaluation Test 5 - Evaluation of Reduction of Porphyromonas gulae -) The plaque suspension obtained in the above evaluation test 1 was quantitatively measured by real-time PCR to determine the number of Porphyromonas gulae bacteria. Table 8 shows the number of Porphyromonas gulae bacteria as DNA amount (ng / μL) per 1 μL of extracted DNA solution. In the calculation of the average value, values ​​below the detection limit (<4) were treated as 0.

[0072] [Table 8]

[0073] Table 8 shows that the number of Porphyromonas gulae bacteria in the treatment group decreased to about 1 / 2 to 1 / 10 of that in the control group one month after the start of the evaluation study.

[0074] The present invention may comprise the following items. [Section 1] An animal oral bacteria reducing agent containing seaweed powder as an active ingredient, The aforementioned seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of seaweed, and contains 0.01 mass% or more of iodine. An animal oral bacteria reducing agent that reduces the number of oral bacteria by spreading the aforementioned animal oral bacteria reducing agent throughout the oral cavity of an animal. [Section 2] The oral bacteria reducing agent for animals described in item 1 above, wherein the aforementioned oral bacteria of animals are selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, Treponema denticola, and Porphyromonas gulae, one or more species. [Section 3] The oral bacteria reducing agent for animals described in item 1 above, wherein all of the oral bacteria of animals are selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, and Treponema denticola, and the number of the selected oral bacteria is simultaneously reduced. [Section 4] The aforementioned seaweed powder is an oral bacteria reducing agent for animals as described in item 1 above, wherein the pH is between 5 and 8. [Section 5] A toothpaste for reducing oral bacteria in animals, comprising the animal oral bacteria reducing agent described in item 1 above as an active ingredient in toothpaste or liquid toothpaste. [Section 6] A mouthwash for reducing oral bacteria in animals, comprising the animal oral bacteria reducing agent described in item 1 above as an active ingredient in a mouthwash solution. [Section 7] A gum for reducing oral bacteria in animals, in which the animal oral bacteria reducing agent described in item 1 above is incorporated as an active ingredient. [Section 8] An animal oral bacteria reducing gel, comprising the animal oral bacteria reducing agent described in item 1 above as an active ingredient in a gel. [Section 9] The process of distributing the animal oral bacteria reducing agent described in item 1 above throughout the animal's oral cavity, The process of spreading the contents of the oral cavity is repeated for a certain period of time. A method for reducing oral bacteria in animals, including the method described above. [Section 10] The method for reducing oral bacteria in animals according to item 9, wherein the step of distributing the animal oral bacteria reducing agent to the oral cavity includes distributing 0.8 g or more of the animal oral bacteria reducing agent to the oral cavity per day.

Claims

1. An animal oral bacteria reducing agent containing seaweed powder as an active ingredient, The aforementioned seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of seaweed, and contains 0.01 mass% or more of iodine. An animal oral bacteria reducing agent that reduces the number of oral bacteria by spreading the aforementioned animal oral bacteria reducing agent throughout the oral cavity of an animal.

2. The oral bacteria reducing agent for animals according to claim 1, wherein the oral bacteria of the animal are selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, Treponema denticola, and Porphyromonas gulae.

3. The oral bacteria reducing agent for animals according to claim 1, wherein the oral bacteria of the animal are all selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, and Treponema denticola, and the number of the selected oral bacteria is simultaneously reduced.

4. The aforementioned seaweed powder has a pH of 5 or more and 8 or less, as described in claim 1, for reducing oral bacteria in animals.

5. A toothpaste for reducing oral bacteria in animals, comprising the animal oral bacteria reducing agent described in claim 1 as an active ingredient in toothpaste or liquid toothpaste.

6. A mouthwash for reducing oral bacteria in animals, comprising the animal oral bacteria reducing agent described in claim 1 as an active ingredient in a mouthwash solution.

7. A gum for reducing oral bacteria in animals, comprising the animal oral bacteria reducing agent described in claim 1 as an active ingredient.

8. An animal oral fungus reducing gel, comprising the animal oral fungus reducing agent described in claim 1 as an active ingredient in the gel.

9. A step of distributing the animal oral bacteria reducing agent described in claim 1 throughout the animal's oral cavity, The process of spreading the contents of the oral cavity is repeated for a certain period of time. A method for reducing oral bacteria in animals, including the method described above.

10. The method for reducing oral bacteria in animals according to claim 9, wherein the step of distributing the animal oral bacteria reducing agent to the oral cavity comprises distributing 0.8 g or more of the animal oral bacteria reducing agent to the oral cavity per day.

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