Cell sorting device

Abstract

The present invention provides a cell sorting device. The cell sorting device comprises a main body, a placement mechanism including a first placement member for placing a sorting target member containing cells to be sorted, an imaging mechanism for acquiring image information of the sorting target cells placed on the placement mechanism, a display mechanism for displaying the image information of the sorting target cells acquired by the imaging mechanism, a selection mechanism for aspirating cells that meet predetermined requirements within the sorting target member, and a control mechanism for controlling the aspiration and release of cells that meet predetermined requirements. By aspirating cells that meet predetermined requirements using the selection mechanism, the sorting efficiency of the cell sorting device is improved, enabling high-throughput sorting of cells.

Description

Technical Field

[0001] This application relates to the field of cell sorting, and particularly to a cell sorting device.

Background Art

[0002] With the development of the biomedical field, cell sorting technology has emerged. By separating a specific cell subset from a mixed cell sample according to the characteristics of the cells, functional analysis of specific cells, particularly the sorting and extraction of single cells, can be realized.

[0003] In related technologies, the methods for sorting single cells mainly include the pipette method, the limiting dilution method, fluorescence-activated cell sorting technology, etc. However, the pipette method and the limiting dilution method are low-throughput and labor-intensive single cell separation methods, and the fluorescence-activated cell sorting technology is difficult to control the cell sorting accuracy and accuracy, resulting in a decrease in cell sorting efficiency.

Summary of the Invention

[0004] According to each embodiment of the present application, a cell sorting device is provided, which includes a main body, a placement mechanism configured to be attached to the main body, the placement mechanism including a first placement member on which a sorting target member containing sorting target cells is placed and which moves relative to the main body to move the sorting target member to a predetermined area relative to the main body; an imaging mechanism attached to the main body and configured to acquire image information of the sorting target cells placed on the placement mechanism, the imaging mechanism including an objective lens configured to perform imaging on the sorting target cells in a predetermined area; a display mechanism for displaying the image information of the sorting target cells acquired by the imaging mechanism; a selection mechanism configured to be attached to the main body and to suck cells that meet a predetermined requirement in the sorting target member, the selection mechanism including a suction and discharge member that moves relative to the main body and is configured to suck and discharge cells that meet the predetermined requirement; and a control mechanism configured to be attached to the main body and to control the movement of the placement mechanism relative to the main body, the movement of the suction and discharge member relative to the main body, and the suction and discharge of cells that meet the predetermined requirement by the suction and discharge member.

[0005] The above-described cell sorting device, through its control mechanism, first controls the imaging mechanism and display mechanism to select target cells from the target cells, and then controls the selection mechanism to aspirate and release cells that meet predetermined requirements. This enables automatic aspiration, release, and recovery of target cells, improving the efficiency and accuracy of cell sorting and achieving high-throughput cell sorting.

[0006] In one embodiment, the placement mechanism includes a placement assembly on which the member to be sorted is placed, and a placement movement assembly, wherein the placement assembly is positioned on the placement movement assembly, and the placement movement assembly is driven to move the placement assembly so that the member to be sorted can be positioned between the suction / discharge member and the objective lens.

[0007] In one embodiment, the mounting assembly is positioned in the mounting and moving assembly and includes a storage means for collecting cells that meet a predetermined requirement, the mounting and moving assembly is driven to move the storage means so that the storage means can be positioned between the suction / discharge member and the objective lens.

[0008] In one embodiment, the cell sorting apparatus further includes a fixing mechanism for fixing a bioculture chip, which is configured as a sorting target component for containing cells to be sorted, to a mounting mechanism.

[0009] In one embodiment, the fixing mechanism includes a chip fixing assembly and a mounting position limiting assembly, the chip fixing assembly includes a chip housing member for housing a bioculture chip and is placeable on the mounting assembly, and the mounting position limiting assembly is installed on the mounting assembly and can fix the chip housing member to the mounting assembly.

[0010] In one embodiment, the imaging mechanism includes an imaging illumination means, an optical path assembly, and a detection assembly. The imaging illumination means is connected to a selection mechanism and used to illuminate the members to be selected and the optical path assembly. The members to be selected are positioned between the imaging illumination means and the optical path assembly. The optical path assembly is used to image the cells to be selected and transmit image information of the cells to be selected to the detection assembly. The detection assembly is connected to a display mechanism and used to acquire image information of the cells to be selected acquired by the optical path assembly.

[0011] In one embodiment, the imaging mechanism further includes a fluorescence assembly, which is located on the same side as the optical path assembly and the cells to be sorted, and includes an excitation light source and at least one filter means for filtering the excitation light emitted from the excitation light source, so that the light emitted from the excitation light source and passing through the filter means causes the cells to be sorted to emit fluorescence.

[0012] In one embodiment, the selection mechanism further includes a mounting assembly, a selection and movement assembly, and a retrieval assembly attached to the main body, wherein the mounting assembly is to which the suction and discharge member is attached; the selection and movement assembly is driven to move the suction and discharge member, thereby moving the suction and discharge member closer to or further away from the mounting mechanism; and the retrieval assembly is connected to the suction and discharge member and can cause the suction and discharge member to aspirate cells that meet predetermined requirements.

[0013] In one embodiment, the selection mechanism is configured to be attached to a mounting assembly and further includes clamping means for clamping and releasing a suction / discharge member, the clamping means including at least one clamping member that moves between a clamping position and a release position, where the clamping member clamps at least a portion of the suction / discharge member, and where the clamping member releases the suction / discharge member.

[0014] In one embodiment, the clamping means further includes a first clamping drive member and a clamping body configured to which the first clamping drive member and the clamping member are attached, wherein the first clamping drive member is configured to drive the clamping member between a clamping position and a release position.

[0015] In one embodiment, the clamping member is configured as an elastic member located between a first clamping drive member and a clamping body, and the elastic member is configured to clamp or release the suction / release member by changing the elastic deformation of the elastic member in the radial direction by adjusting the displacement between the first clamping drive member and the clamping body.

[0016] In one embodiment, the mounting assembly includes a housing member for housing a clamping means and at least one fixing member supported by the housing member, the fixing member fixing the clamping means to the housing member.

[0017] In one embodiment, the selection mechanism further includes a selection illumination means connected to a mounting assembly and located at the end of the suction / discharge member away from the first mounting member, wherein light emitted from the selection illumination means can pass through the suction / discharge member.

[0018] In one embodiment, a hollow conduit is installed in the suction and discharge member, and the selective illumination means includes a selective illumination light source installed at one end of the suction and discharge member so as to extend in the axial direction of the suction and discharge member, and light emitted from the selective illumination light source can pass through the hollow conduit of the suction and discharge member to form a projection of the suction and discharge member.

[0019] In one embodiment, the cleaning assembly further includes a cleaning liquid means for cleaning a suction discharge member and a driving means, wherein a hollow conduit is formed in the suction discharge member, the cleaning liquid means includes a first cleaning liquid and a second cleaning liquid different from the first cleaning liquid, and the driving means is connected to the suction discharge member and is configured to draw the first cleaning liquid into the hollow conduit to clean the suction discharge member a first predetermined number of times, and to draw the second cleaning liquid into the hollow conduit to clean the suction discharge member a second predetermined number of times. [Brief explanation of the drawing]

[0020] To more clearly illustrate the embodiments of the present application or the technical configurations of the prior art, the drawings necessary for the description of the embodiments or the prior art will be briefly introduced below. The drawings described below are only the embodiments of the present application, and it is self-evident that those skilled in the art can also obtain other drawings from the disclosed drawings without creative effort. [Figure 1] It is a schematic diagram of a cell sorting device in some embodiments of the present application. [Figure 2] It is an axonometric view of the housing assembly of the cell sorting device in some embodiments of the present application removed. [Figure 3] It is a front view of the cell sorting device in FIG. 2 with the display mechanism removed. [Figure 4] It is an enlarged view of the selection mechanism in FIG. 3. [Figure 5] It is an axonometric view of the selection mechanism in FIG. 4. [Figure 6] It is a right side view of the selection mechanism in FIG. 4. [Figure 7] It is a schematic diagram of the suction and discharge member in FIG. 2. [Figure 8] It is an axonometric view of the mounting assembly and the lighting assembly of the cell sorting device in some embodiments of the present application. [Figure 9] It is a front view full cross-sectional view of the mounting assembly and the lighting assembly of the cell sorting device in some embodiments of the present application. [Figure 10] It is a plan view full cross-sectional view of the mounting assembly and the lighting assembly in some embodiments of the present application. [Figure 11] It is an axonometric view of the clamping means of the suction and discharge member in some embodiments of the present application. [Figure 12] It is a cross-sectional view of the clamping means of the suction and discharge member in some embodiments of the present application. [Figure 13] It is an axonometric view of the first clamping drive member of the clamping means in FIG. 11. [Figure 14] It is an axonometric view of the clamping body of the clamping means in FIG. 11. [Figure 15]Figure 11 is a cross-sectional view of the clamping body of the clamping mechanism. [Figure 16] Figure 11 is an axial view of the second clamping drive member of the clamping mechanism. [Figure 17] This is a plan view of the housing member for housing the clamping means shown in Figure 8. [Figure 18] This is a cross-sectional view of the housing member for housing the clamping means shown in Figure 8. [Figure 19] These are axial views of the mounting mechanism in some embodiments of the present application. [Figure 20] Figure 19 is a front view of the mounting mechanism. [Figure 21] Figure 19 is an axial view of the mounting assembly in the mounting mechanism. [Figure 22] This is a front view of the first mounting member in the mounting mechanism shown in Figure 19. [Figure 23] Figure 19 is an axial view of the second mounting member and the first mounting movement means in the mounting mechanism. [Figure 24] Figure 19 is an axial view of the third mounting member and the second mounting movement means in the mounting mechanism. [Figure 25] This is an axial view of the storage means of a cell sorting device in some embodiments of the present invention. [Figure 26] Figure 25 is an axial view of the reaction tube of the storage means. [Figure 27] This is an axial diagram of the imaging mechanism of a cell sorting device in several embodiments of the present invention. [Figure 28] This is a schematic diagram of the imaging mechanism of a cell sorting device in several embodiments of the present invention. [Figure 29] This is a schematic diagram of the control mechanism of a cell sorting device in some embodiments of the present invention. [Figure 30] This is a schematic diagram of a bioculture chip in several embodiments of the present invention. [Figure 31] This is a schematic diagram of the bioculture chip containment chamber in some embodiments of the present invention. [Figure 32]This is a schematic diagram of the containment chamber for a bioculture chip in some embodiments of the present invention, wherein the opening diameter of the containment chamber is smaller than the bottom diameter of the containment chamber. [Figure 33] This is a schematic diagram of the chip fixing assembly of a cell sorting device in some embodiments of the present invention. [Figure 34] This is a schematic diagram of a pressurizing member in several embodiments of the present invention. [Figure 35] This is a cross-sectional view of the clamping means in some embodiments of the present application, wherein the spacer, the second clamping drive member, and the clamping body are integrally molded. [Figure 36] This is a plan view of a mounting assembly in several embodiments of the present application, wherein the fixing means includes one fixing member. [Figure 37] This is a schematic diagram of the projection mapping of the suction discharge member and the operation of the projection marker of the suction discharge member projection identification unit in some embodiments of the present invention. [Modes for carrying out the invention]

[0021] The technical modes of the embodiments of this application will be clearly and completely described below with reference to the drawings of the embodiments. It is clear that the embodiments described are only a selection of the embodiments of this application and do not encompass all embodiments. Any other embodiments obtained by a person skilled in the art without creative work based on the embodiments of this application are all within the scope of protection of this application.

[0022] In the description of this application, terms such as "center," "vertical," "horizontal," "length," "width," "thickness," "top," "bottom," "front," "back," "left," "right," "vertical," "horizontal," "top," "bottom," "inside," "outside," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" are based on the orientation or positional relationship shown in the drawings and are used merely for the convenience and simplification of the description of this application, and do not express or suggest that the devices or elements mentioned must have a specific orientation or must be configured and operated in a specific direction. Therefore, they should not be interpreted as limitations on this application.

[0023] Furthermore, terms such as “first,” “second,” etc., are used merely for descriptive purposes and should not be interpreted as expressing or suggesting relative importance or implicitly defining the number of technical features being referred to. Accordingly, features limited by “first” or “second” express or implicitly indicate that they include at least one such feature. In this description, unless otherwise specified, the term “plural” means at least two, for example, two, three, etc.

[0024] In this application, unless otherwise specified, terms such as "attach," "connect," "connect," and "fix" should be interpreted broadly. For example, unless otherwise specified, it may refer to a fixed connection, a detachable connection, or an integrated structure. It may refer to a mechanical connection or an electrical connection. It may refer to a direct connection or an indirect connection via an intermediate medium. It may refer to internal communication between two elements or interaction between two elements. A person skilled in the art will be able to appropriately understand the specific meaning of these terms in this application depending on the specific situation.

[0025] In this application, unless otherwise specified, any description such as "the first feature is above or below the second feature" means that the first and second features are in direct contact or indirectly in contact through an intermediate medium. Furthermore, a description that the first feature is "above," "above," or "on the upper side" of the second feature means that the first feature is directly above or diagonally above the second feature, or simply that the height of the first feature is greater than that of the second feature. A description that the first feature is "below," "below," or "on the lower side" of the second feature means that the first feature is directly below or diagonally below the second feature, or simply that the height of the first feature is lower than that of the second feature.

[0026] When an element is said to be “fixed” or “installed” to another element, that element may be directly located to the other element, or an intermediate element may be present. When an element is considered to be “connected” to another element, that element may be directly connected, or an intermediate element may be present. The terms and similar expressions used in this application, such as “vertical,” “horizontal,” “up,” “down,” “left,” and “right,” are for illustrative purposes only and do not represent only one embodiment.

[0027] Cell sorting is a technique for separating cells that meet specific requirements from a group of target cells, based on the characteristics of the cells. When functional analysis of cells that meet specific requirements is necessary, such as detecting cell molecules by analyzing cell culture supernatant or detecting cell function through cell co-culture, obtaining high-purity cells that meet the specified requirements is a prerequisite.

[0028] Cell sorting devices automatically select, separate, and purify cells that meet predetermined conditions from a set of target cells. They offer advantages such as multi-species compatibility, high throughput, multiple sorting, short sorting cycles, and high sorting accuracy. Furthermore, compared to conventional manual sorting, cell sorting using a cell sorting device reduces the influence of human factors, ensures cell uniformity in the sorting process, and allows for the accurate acquisition of single cells with perfect morphology and high activity, thereby improving sorting efficiency and enabling high-throughput sorting of single cells.

[0029] The following describes in detail some of the cell sorting devices in the embodiments of this application.

[0030] Figures 1 to 3 show schematic diagrams of the cell sorting apparatus 100 in several embodiments of the present invention. For the sake of comparison and explanation, in Figure 3, the direction perpendicular to the plane of the paper is defined as the first direction S1, the left-right direction is defined as the second direction S2, and the up-down direction is defined as the third direction S3. The first direction S1, the second direction S2, and the third direction S3 are perpendicular to each other.

[0031] Referring to Figures 1 and 2, the main body 10 is the main part of the cell sorting device 100 and is used to support and house the main components of the cell sorting device 100. The main body 10 includes a housing assembly 11 and a support assembly 12. The housing assembly 11 forms the housing space of the cell sorting device 100, and the support assembly 12 supports the mechanism installed in the housing space. Referring to Figures 2 and 3, in some embodiments, the cell sorting device 100 includes the main body 10, a selection mechanism 20, a placement mechanism 30, an imaging mechanism 40, a display mechanism 50, and a control mechanism 60. The main body 10 is used to support and house the other mechanisms of the cell sorting device 100 and forms the housing space for the selection mechanism 20, the placement mechanism 30, the imaging mechanism 40, and the control mechanism 60. The placement mechanism 30 is used to place a sorting target member containing the cells to be sorted. The imaging mechanism 40 is used to image the cells to be sorted and obtain image information of the cells to be sorted. The display mechanism 50 is used to display image information of the cells to be sorted acquired by the imaging mechanism 40. The image information of the cells to be sorted is processed and cells that meet predetermined requirements within the sorting target member are selected. The selection mechanism 20 is used to aspirate and collect the cells that meet the predetermined requirements within the sorting target member, thereby achieving aspiration and collection of cells that meet the predetermined requirements and completing cell sorting.

[0032] The housing assembly 11 is primarily used to form the containment space for the internal mechanism of the cell sorting device 100, to prevent light and foreign matter from entering the containment space from the outside, to provide a sterile environment for the cell sorting process, to prevent cell contamination, and to ensure cell activity and normal growth.

[0033] The housing assembly 11 has a hollow structure as a whole and is used to house the selection mechanism 20, mounting mechanism 30, imaging mechanism 40, and control mechanism 60. Typically, the housing assembly 11 is made of a rigid material such as sheet metal, glass, or hard plastic. The specific material and light transmittance of the housing assembly 11 are determined based on the housing's location and required function. For example, a housing that provides overall protection can be made of sheet metal, while a housing requiring light shielding can be made of a light-shielding material.

[0034] In some embodiments, referring to Figure 1, the housing assembly 11 includes a first housing 111 and a second housing 112. The first housing 111 can move along a second direction S2 relative to the second housing 112. The movement of the first housing 111 allows the members to be sorted to be placed on the mounting mechanism 30 or removed from the cell sorting device 100.

[0035] The first housing 111 is made of a light-transmitting material and, during use, allows observation of the operating status of the cell sorting device 100 by passing light through the first housing 111. To avoid interference between external light and the image information of the cells to be sorted acquired by the cell sorting device 100, and to observe the operating status inside the cell sorting device 100, the first housing 111 is made of a material with low light transmittance, such as glass or plastic, or for example, an acrylic plate.

[0036] The second housing 112 is primarily used to protect the internal components of the cell sorting device 100 and is entirely constructed of light-shielding sheet metal or the like. In some embodiments, the second housing 112, which is in contact with the first housing 111, is made of plastic to prevent damage to the second housing 112 when the first housing 111 is opened, while also preventing contact damage to the sorting target components or the user during removal. Furthermore, the second housing 112 is provided with heat dissipation holes to allow the cell sorting device 100 to dissipate heat. The heat dissipation holes release the heat generated during the operation of the cell sorting device 100, thereby avoiding the effect of heat on the activity of the sorting target cells, contributing to the long-term use of the cell sorting device 100, and enabling high-throughput sorting of cells.

[0037] Referring again to Figures 2 and 3, the support assembly 12 is used to support the main components of the cell sorting device 100 and includes a base 121 and a support seat 122. The base 121 is used to mount the main components of the cell sorting device 100. The support seat 122 is connected to the base 121 along the third direction S3 and is used to connect and support the main components of the cell sorting device 100.

[0038] The base 121 includes a base plate 1211 and support legs 1212, the support legs 1212 being connected to the base plate 1211 along a third direction S3. By adjusting the relative position of the support legs 1212 to the base plate 1211 in the third direction S3, the overall horizontality of the cell sorting device 100 can be adjusted, thereby allowing the cell sorting device 100 to be used on different desk surfaces, platforms, etc. Furthermore, the support legs 1212 also have functions such as vibration damping and anti-slip properties.

[0039] The support seat 122 includes a support plate 1221 and a rib plate 1222. The support plate 1221 is connected to the base plate 1211 along the third direction S3. In order to obtain the largest possible storage space, the support plate 1221 is installed on one side of the base plate 1211 in the first direction S1. By installing the support plate 1221, it becomes possible to connect and support the main components of the cell sorting device 100 in the third direction S3.

[0040] The rib plates 1222 are used to provide auxiliary support to the support plate 1221, forming a right triangle overall, with one adjacent side extending in a third direction S3 and connected to the support plate 1221 along the first direction S1, and the other adjacent side extending in the first direction S1 and connected to the base plate 1211 along the third direction S3. In some embodiments, the support seat 122 includes a predetermined number of rib plates 1222. In one embodiment, two rib plates 1222 are installed facing each other on both sides of the base plate 1211 in the second direction S2. Installing the rib plates 1222 improves the overall structural stability of the cell sorting device 100.

[0041] To facilitate the assembly and replacement of the support plate 1221, base plate 1211, and rib plate 1222, the support plate 1221, base plate 1211, and rib plate 1222 are detachably connected to each other, for example, by fastening members. Typically, detachable connections include, but are not limited to, screw connections, threaded connections, snap-type movable connections, clamp connections, flange connections, pin-type movable connections, and socket connections. The method of connecting the support plate 1221, base plate 1211, and rib plate 1222 is not limited; for example, the rib plate 1222 can be fixedly connected to the base plate 1211 and support plate 1221 by welding.

[0042] Referring to Figures 4 to 6, selection mechanisms in several embodiments of the present application are shown. The selection mechanism 20 includes a suction / discharge member 21, a mounting assembly 22, a selection / movement assembly 23, a selection / illumination means 24, a washing assembly 25, and a recovery assembly 26. The selection mechanism 20 is used to select cells that meet predetermined requirements from a member to be sorted. By automating the selection mechanism 20, automatic cell aspiration is achieved, improving the efficiency of cell sorting, avoiding damage to cells due to manual operation, contributing to high-throughput cell sorting, and further enabling the sorting of single cells.

[0043] Referring to Figure 7, several embodiments of the present invention are shown. The suction-discharge member 21 is used to aspirate and discharge cells that meet predetermined requirements. The suction-discharge member 21 is provided with a hollow conduit 211 so that cells can enter the suction-discharge member 21 and the aspirated cells can be stored within the suction-discharge member 21. At least a portion of the dimensions of the suction-discharge member 21 gradually decrease along the radial direction of the suction-discharge member 21 so that a suction-discharge port 212 with dimensions suitable for the cells to be aspirated is formed at one end of the suction-discharge member 21. When aspirated, cells enter the hollow conduit 211 through the suction-discharge port 212 and when discharged, they pass through the hollow conduit 211 and leave the suction-discharge port 212. The design of the suction-discharge port 212 facilitates accurate and effective aspiration of cells and can improve the sorting efficiency of the cell sorting device 100. The material and dimensions of the suction / discharge member 21 can be selected according to the dimensions of the cells to be sorted; for example, a 1 mm glass capillary can be used.

[0044] Referring to Figures 8 to 10, mounting assemblies and illumination assemblies of the cell sorting apparatus in several embodiments of the present application are shown. The mounting assembly 22 is used to mount and secure the suction / discharge member 21, preventing the suction / discharge member 21 from falling off during use. Accordingly, the mounting assembly 22 can also be used for replacing the suction / discharge member 21. The mounting assembly 22 includes clamping means 221, fixing means 222, and housing member 223.

[0045] Referring to Figures 11 and 12, the clamping means for the suction / discharge member in several embodiments of the present application are shown. The clamping means 221 is used to clamp and release the suction / discharge member 21 and is connected to the suction / discharge member 21 along a third direction S3. Referring to Figure 7, one end of the suction / discharge member 21 is used for aspirating and releasing cells to be sorted, and a suction / discharge port 212 is formed therein. The other end of the suction / discharge member 21 is clamped or released by the clamping means 221. Clamping by the clamping means 221 allows for stable use of the suction / discharge member 21, and release by the clamping means 221 allows for replacement of the suction / discharge member 21. By installing the clamping means 221, the suction / discharge member 21 can be fixed or released without damaging the suction / discharge member 21.

[0046] Furthermore, the clamping means 221 includes at least one clamping member that is movable between a clamping position and a release position, and the clamping member is used to clamp the other end of the suction discharge member. In the clamping position, the clamping member attaches and fixes the suction discharge member 21 by clamping the other end of the suction discharge member 21. In the release position, the clamping member releases the other end of the suction discharge member 21, allowing the suction discharge member 21 to be recovered.

[0047] In some embodiments, the clamping means 221 includes a clamping body 2211 and a first clamping drive member 2212. The first clamping drive member 2212 is used to drive the clamping member between a clamping position and a release position. The clamping body 2211 is used to support the first clamping drive member 2212. Furthermore, the first clamping drive member 2212 is provided with an opening for the passage of a suction / discharge member 21. At least a portion of the opening diameter of the first clamping drive member 2212 is formed to be slightly larger than the outer diameter of the suction / discharge member 21 so that the suction / discharge member 21 can pass through the first clamping drive member 2212.

[0048] In some embodiments, the clamping body 2211 and the first clamping drive member 2212 are detachably connected to facilitate the installation and replacement of the suction / discharge member 21. Specifically, the clamping body 2211 and the first clamping drive member 2212 are screwed together. The clamping body 2211 is provided with a female thread, and the first clamping drive member 2212 is provided with a male thread, and the connection between the female and male threads realizes the connection between the first clamping drive member 2212 and the clamping body 2211. Screwing is a convenient and stable connection method, and facilitates the installation and detachment of the first clamping drive member 2212.

[0049] In other embodiments, the clamping body 2211 and the first clamping drive member 2212 are fitted in an interference fit. This interference fit improves the stability of the assembly of the clamping body 2211 and the first clamping drive member 2212 and facilitates the fixing of the suction / discharge member 21 by the clamping member. The interference fit between the first clamping drive member 2212 and the clamping body 2211 is achieved by making the outer diameter of at least a portion of the first clamping drive member 2212 greater than or equal to the inner diameter of the clamping body 2211.

[0050] In some embodiments, the first clamping drive member 2212 is elastically deformable in at least a portion. When the first clamping drive member 2212 and the clamping body 2211 are connected, the first clamping drive member 2212 moves from the release position to the clamping position, and the outer wall of the first clamping drive member 2212 contacts and presses against the inner wall of the clamping body 2211, causing elastic deformation of the first clamping drive member 2212. As a result, the inner wall of the first clamping drive member 2212 contacts and presses against the outer wall of the suction / discharge member 21, thereby achieving clamping and fixing of the suction / discharge member 21 by the clamping means 221. In this embodiment, the first clamping drive member 2212 is configured as a clamping member.

[0051] In other embodiments, the inner wall dimensions of at least a portion of the first clamping drive member 2212 are made variable, for example, the inner wall of the first clamping drive member 2212 is made a cam surface. When the first clamping drive member 2212 and the clamping body 2211 are connected, the first clamping drive member 2212 moves from the release position to the clamping position, and the inner wall dimensions of the first clamping drive member 2212 change, causing the inner wall of the first clamping drive member 2212 to contact and press against the outer wall of the suction / discharge member 21, thereby fixing the suction / discharge member 21 in the clamping means 221. In this embodiment, the first clamping drive member 2212 is configured as a clamping member.

[0052] In some embodiments, the clamping means 221 includes a first clamping drive member 2212, a second clamping drive member 2213, and a clamping body 2211. The second clamping drive member 2213 is inserted through the suction / discharge member 21, and the suction / discharge member 21 is accommodated by forming at least a portion of the inner diameter of the second clamping drive member 2213 to be slightly larger than the outer diameter of the suction / discharge member 21. The second clamping drive member 2213 is positioned between the first clamping drive member 2212 and the clamping body 2211 in a third direction S3. By installing the first clamping drive member 2212 and the second clamping drive member 2213, at least two locations of the suction / discharge member 21 are accommodated, improving the mounting stability of the suction / discharge member 21.

[0053] In some embodiments, the second clamping drive member 2213 and the clamping body 2211 are fixedly connected, for example, by welding the second clamping drive member 2213 to the clamping body 2211, or by integrally molding the second clamping drive member 2213 with the clamping body 2211. In other embodiments, the method of connecting the second clamping drive member 2213 and the clamping body 2211 may be one or more of gap coupling, clamping coupling, elastic coupling, and surface coupling. The second clamping drive member 2213 and the clamping body 2211 may be screwed together. It should be understood that the method of connecting the second clamping drive member 2213 and the clamping body 2211 is not limited as long as the housing and setting of the second clamping drive member 2213 can be achieved.

[0054] In some embodiments, one end of the second clamping drive member 2213 in the third direction S3 is housed in the first clamping drive member 2212, and the inner diameter of at least a portion of the first clamping drive member 2212 is formed to be slightly larger than the outer diameter of the second clamping drive member 2213. The other end of the second clamping drive member 2213 in the third direction S3 is housed in the clamping body 2211, and the inner diameter of at least a portion of the clamping body 2211 is formed to be slightly larger than the outer diameter of the second clamping drive member 2213. Since at least two points of the second clamping drive member 2213 are housed and fixed, this contributes to the mounting stability of the second clamping drive member 2213.

[0055] In some embodiments, the second clamping drive member 2213 is elastically deformable in at least a portion. When the second clamping drive member 2213 and the first clamping drive member 2212 are connected, the outer wall of the second clamping drive member 2213 contacts and presses against the inner wall of the first clamping drive member 2212, causing elastic deformation in the second clamping drive member 2213. This causes the inner wall of the second clamping drive member 2213 to contact and press against the outer wall of the suction / discharge member 21, thereby fixing the suction / discharge member 21 in the second clamping drive member 2213. In this embodiment, the second clamping drive member is configured as a clamping member.

[0056] In some embodiments, the inner wall dimensions of at least a portion of the second clamping drive member 2213 are made variable, for example, the inner wall of the second clamping drive member 2213 is made a cam surface. When the second clamping drive member 2213 and the first clamping drive member 2212 are connected, the inner wall dimensions of the second clamping drive member 2213 change, so that the inner wall of the second clamping drive member 2213 contacts and presses against the outer wall of the suction / discharge member 21, thereby fixing the suction / discharge member 21 in the clamping means 221. In this embodiment, the second clamping drive member 2213 is configured as a clamping member.

[0057] In some embodiments, the clamping means 221 includes a first clamping drive member 2212, a clamping body 2211, and a first elastic member 2214. The first elastic member 2214 is positioned between the first clamping drive member 2212 and the clamping body 2211 in a third direction S3. In its natural state, the inner diameter of the first elastic member 2214 is formed to be slightly larger than the outer diameter of the suction / discharge member 21. In this embodiment, the first elastic member 2214 is configured as a clamping member. By adjusting the displacement between the first clamping drive member 2212 and the clamping body 2211, the elastic deformation of the first elastic member 2214 in the radial direction is changed, thereby achieving clamping or release of the suction / discharge member 21 by the first elastic member 2214. Specifically, a male screw is installed on the first clamping drive member 2212, and a female screw is installed on the clamping body 2211. By adjusting the tightening or loosening of the male screw and the female screw, the elastic deformation of the first elastic member 2214 in the radial direction is changed, thereby achieving clamping or release of the suction / discharge member 21 by the first elastic member 2214.

[0058] In some embodiments, the clamping means 221 includes a first clamping drive member 2212, a clamping body 2211, a second clamping drive member 2213, and a first elastic member 2214. The first elastic member 2214 is positioned between the first clamping drive member 2212 and the second clamping drive member 2213 in a third direction S3. When assembling the clamping means 221, the elastic deformation of the first elastic member 2214 in the radial direction is changed by adjusting the displacement between the first clamping drive member 2212 and the second clamping drive member 2213. The first elastic member 2214 is configured as the first clamping member. Specifically, the first clamping drive member 2212 and the second clamping drive member 2213 press against the first elastic member 2214 with their opposing end faces in the third direction S3, thereby deforming the first elastic member 2214 radially and tightening and fixing the suction / discharge member 21.

[0059] In this embodiment, the clamping body 2211 or the second clamping drive member 2213 is configured as a first hollow structure that houses the suction and discharge member 21, and the first clamping drive member 2212 is configured as a second hollow structure that houses the suction and discharge member 21. The suction and discharge member 21 is supported and clamped by the cooperative action of the first hollow structure, the second hollow structure and the first clamping member 2214.

[0060] Furthermore, the clamping means 221 further includes a spacer 2215 positioned between the second clamping drive member 2213 and the first elastic member 2214 in the third direction S3. The spacer 2215 is inserted through the suction / discharge member 21, i.e., the inner diameter of the spacer 2215 is formed to be slightly larger than the outer diameter of the suction / discharge member 21. By installing the spacer 2215, at least three locations of the suction / discharge member 21 are accommodated, and the second clamping drive member 2213 can accommodate the mounting of different suction / discharge members 21.

[0061] In some embodiments, referring to Figure 35, the spacer 2215, the second clamping drive member 2213, and the clamping body 2211 are integrally molded. By adjusting the displacement between the first clamping drive member 2212 and the clamping body, the displacement between the spacer 2215 and the first elastic member 2214 is changed, thereby changing the elastic deformation of the first elastic member 2214 in the radial direction, and achieving clamping or release of the suction / discharge member 21 by the first clamping member 2214.

[0062] Furthermore, the clamping means 221 has a first elastic member 2214 and a second elastic member 2216 arranged at a distance from each other in the third direction S3, and the first elastic member 2214 and the second elastic member 2214 are positioned at both ends of the spacer 2215 in the third direction S3. Specifically, the first elastic member 2214 is positioned between the first clamping drive member 2212 and the spacer 2215, and the second elastic member 2216 is positioned between the second clamping drive member 2213 and the spacer 2215. By adjusting the displacement between the first clamping drive member 2212 and the spacer 2215, the elastic deformation of the first elastic member 2214 in the radial direction is changed, and by adjusting the displacement between the second clamping drive member 2213 and the spacer 2215, the elastic deformation of the second elastic member 2216 in the radial direction is changed, thereby enabling clamping or release of the suction / discharge member 21 by the first elastic member 2214 and the second elastic member 2216. In this embodiment, the first elastic member 2214 is configured as the first clamping member, and the second elastic member 2216 is configured as the second clamping member. By installing the first elastic member 2214 and the second elastic member 2216, at least two points of the suction / discharge member 21 are tightened, ensuring the mounting stability of the suction / discharge member 21.

[0063] Specifically, a male screw is installed on the first clamping drive member 2212, and a female screw is installed on the clamping body 2211. By adjusting the tightening or loosening of the male and female screws, the elastic deformation of the first clamping member 2214 and the second clamping member 2216 in the radial direction is changed, thereby achieving clamping or release of the suction / discharge member by the first clamping member 2214 and the second clamping member 2216.

[0064] The second clamping drive member 2213 is configured as a first hollow structure that houses the suction and discharge member 21, the first clamping drive member 2212 is configured as a second hollow structure that houses the suction and discharge member 21, and the spacer 2215 is configured as a third hollow structure that houses the suction and discharge member 21. The suction and discharge member 21 is supported and clamped by the cooperative action of the first hollow structure, the second hollow structure, the third hollow structure, the first clamping member 2214, and the second clamping member 2216.

[0065] In some specific embodiments, with reference to Figure 13, the first clamping drive member of the clamping means in some embodiments of the present application is shown. The first clamping drive member 2212 includes a first clamping drive section 2212a, an operating section 2212b, and a second clamping drive section 2212c. The first clamping drive section 2212a is provided with a through hole for accommodating at least a portion of the second clamping drive member 2213, a first elastic member 2214, a spacer 2215, and a second elastic member 2216. The first clamping drive section 2212a is provided with a male thread, and the first clamping drive member 2212 is connected to the female thread of the clamping body 2211 by the male thread of the first clamping drive section 2212a. The inner diameter of the first clamping drive unit 2212a is formed to be slightly larger than the outer diameter of at least a portion of the second clamping drive member 2213, and the outer diameter of at least a portion of the first clamping drive unit 2212a is formed to be slightly larger than the inner diameter of the clamping body 2211. The second clamping drive unit 2212c is used to house the suction / discharge member 21, and its inner diameter is formed to be slightly larger than the outer diameter of the suction / discharge member 21.

[0066] The operating section 2212b is provided so as to protrude radially from the first clamping drive section 2212a and the second clamping drive section 2212c, and is positioned between the first clamping drive section 2212a and the second clamping drive section 2212c. The inner diameter of the operating section 2212b is formed to be slightly larger than the outer diameter of the suction / discharge member 21. The operating section 2212b cooperates with an external tool and enables the connection between the first clamping drive member 2212 and the clamping body 2211. Specifically, the outer circumferential surface of the operating section 2212b includes an arc-shaped portion and a flat portion that are installed opposite each other. The flat portion cooperates with an external tool such as a wrench to enable the connection between the male thread of the first clamping drive member 2212 and the female thread of the clamping body 2211, thereby enabling the clamping of the suction / discharge member 21.

[0067] In some embodiments, the clamping body of the clamping means in some embodiments of the present application is shown with reference to Figures 14 and 15. The clamping body 2211 includes a first clamping body portion 2211a and a second clamping body portion 2211b connected to the first clamping body portion 2211a. By installing the first clamping body portion 2211a, a connection is made between the hollow conduit 211 of the suction discharge member 21 and the recovery assembly 26, thereby enabling cleaning of the suction discharge member 21 and the aspiration and release of cells that meet predetermined requirements. The first clamping body portion 2211a is equipped with a first housing chamber 2211c, which houses at least a portion of the second clamping drive member 2213, thereby enabling communication between the first housing chamber 2211c and the hollow conduit 211 of the suction discharge member 21, and also connects to at least a portion of the selective lighting means 24 to illuminate the hollow conduit 211 of the suction discharge member 21. Specifically, the first housing chamber 2211c includes a stepped joint for connecting to the selective lighting means 24 to illuminate the suction discharge member 21. The central axis of the joint coincides with the central axis of the suction discharge member 21 to ensure the illumination effect of the suction discharge member 21 by the selective lighting means 24. It should be understood that the shape of the joint is not limited to a stepped shape and can be adjusted as needed, as long as connection with the selective lighting means 24 can be achieved. Furthermore, the first clamping body portion 2211a has a connecting hole in the radial direction, which communicates with the first containment chamber 2211c and is used for connection with the recovery assembly 26.

[0068] The second clamping body portion 2211b is provided with a second housing chamber 2211d that communicates with the first housing chamber 2211c, to which the first clamping drive member 2212 is connected, and for housing the second clamping drive member 2213. The first clamping drive member 2212 is positioned so that at least a portion of it enters the second housing chamber 2211d, allowing at least a portion of it to be housed and connected to the clamping body 2211. At least a portion of the first clamping drive member 2212 protrudes into the second housing chamber 2211d, forming an operating portion 2212b for operating the first clamping drive member 2212 to move it. At least a portion of the surface of the second clamping body portion 2211b gradually changes along the third direction S3 to form a tapered surface. This makes it easy to fix and adjust the clamping means 221. At least a portion of the inner diameter of the second clamping body 2211b is formed to be slightly larger than the outer diameter of the first clamping drive unit 2212a. Specifically, the second clamping body 2211b is fitted with a female thread, the second clamping drive unit 2212a is fitted with a male thread, and the female thread is connected to the male thread.

[0069] In some embodiments, with reference to Figure 16, a second clamping drive member of the clamping means in some embodiments of the present application is shown. The second clamping drive member 2213 has a hollow structure and is inserted through the suction discharge member 21 to enable the attachment and accommodation of the suction discharge member 21. The second clamping drive member 2213 includes a fourth clamping drive portion 2213a, a second flange portion 2213b, and a fifth clamping drive portion 2213c. The inner diameters of the fourth clamping drive portion 2213a, the second flange portion 2213b, and the fifth clamping drive portion 2213c are formed to be slightly larger than the outer diameter of the suction discharge member 21, thereby enabling the insertion and accommodation of the suction discharge member 21. Referring to Figures 12 and 15, at least a portion of the fourth clamping drive unit 2213a is housed in the first clamping body unit 2211a along the third direction S3, thereby achieving the coupling between the second clamping drive member 2213 and the clamping body 2211. The outer diameter of the fourth clamping drive unit 2213a is formed to be slightly smaller than the inner diameter of at least a portion of the first clamping body unit 2211a. Referring to Figures 12 and 13, at least a portion of the fifth clamping drive unit 2213c is housed in the first clamping drive unit 2212a, thereby achieving the coupling between the second clamping drive member 2213 and the first clamping drive member 2212. The outer diameter of the fifth clamping drive unit 2213c is formed to be slightly smaller than the inner diameter of the first clamping drive unit 2212a.

[0070] The second flange portion 2213b is provided so as to protrude more than the fourth clamping drive portion 2213a and the fifth clamping drive portion 2213c in the third direction S3, and is positioned between the fourth clamping drive portion 2213a and the fifth clamping drive portion 2213c. Referring to Figures 12 and 15, the clamping means 221 further includes a seal 2217 for securing the second clamping drive member 2213. The seal 2217 is positioned between the second flange portion 2213b and the first clamping body portion 2211a in the third direction S3, and the shape and dimensions of the inner diameter of the seal 2217 conform to the shape and dimensions of the outer diameter of the fourth clamping drive portion 2213a. In this embodiment, the seal 2217 is housed in the second housing chamber 2211d and is supported by the second flange portion 2213b. When assembling the clamping means 221, the seal 2217 prevents communication between the first containment chamber 2211c and the second containment chamber 2211d, so that the aspiration or release of cells to be sorted is performed immediately by the aspiration / release member 21 after passing through the first containment chamber 2211c, without passing through the second containment chamber 2211d. This prevents the second containment chamber 2211d from affecting the aspiration or release of cells to be sorted by the aspiration / release member 21. In other embodiments, the seal 2217 may be housed in the first containment chamber 2211c so as to prevent communication between the first containment chamber 2211c and the second containment chamber 2211d.

[0071] The first elastic member 2214, the second elastic member 2216, and the seal 2217 are all annular elastic elements such as O-rings. The shape, dimensions, number, and type of the first elastic member 2214, the second elastic member 2216, and the seal 2217 are not limited.

[0072] Furthermore, in order to facilitate the installation and replacement of the suction and discharge member 21 and to avoid damaging the suction and discharge member 21 during installation, the central axes of the first clamping drive member 2212, the first elastic member 2214, the spacer 2215, the second elastic member 2216, and the second clamping drive member 2213 in the third direction S3 all coincide with the central axis of the suction and discharge member 21 in the third direction S3.

[0073] When assembling the clamping means 221, the first elastic member 2214, the spacer 2215, the second elastic member 2216, and the second clamping drive member 2213 are sequentially positioned on the first clamping drive member 2212 along the third direction S3, with the end of the second clamping drive member 2213 away from the second elastic member 2216 being housed in the first housing chamber 2211c of the first clamping body 2211a along the third direction S3, and the other end of the second clamping drive member 2213 in contact with the second elastic member 2216 being housed in the housing chamber of the first clamping drive member 2212 along the third direction S3.

[0074] When attaching the suction / discharge member 21, the suction / discharge member 21 passes sequentially through the through holes formed in the first clamping drive member 2212, the first clamping member 2214, the spacer 2215, the second clamping member 2216, and the second clamping drive member 2213 along the third direction S3 until its end face becomes flush with the end face of the second clamping drive member 2213 housed in the clamping body 2211. Then, by tightening the first clamping drive member 2212 and elastically deforming the first clamping member 2214 and the second clamping member 2216 in the radial direction, the suction / discharge member 21 is fixed, and by deforming the seal 2217 in the radial direction, the second clamping drive member 2213 is fixed, and the first housing chamber 2211c and the second housing chamber 2211d are sealed. When retrieving or replacing the suction discharge member 21, the first clamping drive member 2212 is loosened, the first clamping member 2214 and the second clamping member 2216 are restored to their original positions, and then the suction discharge member 21 is removed along the opposite direction to the installation direction.

[0075] In some embodiments, referring to Figure 11, a clamping marker 2212d is provided on the first clamping drive member 2212. The clamping marker 2212d is placed on the surface of the first clamping drive member 2212 and is used to clamp the suction / discharge member 21 by rotating the first clamping drive member 2212 to a predetermined position which is the clamping position. Since the suction / discharge member is designed to be a glass capillary, providing the clamping marker 2212d effectively prevents damage to the glass capillary due to excessive tightening of the first clamping drive member 2212. In other embodiments, the clamping marker 2212d corresponds to a marker located on the clamping body 2211 or other parts, and the position where the two coincide is the clamping position.

[0076] Referring again to Figures 8 to 10, in some embodiments, the fixing means 222 is installed along the radial direction of the clamping means 221 and is used to fix and adjust the clamping means 221. The fixing means 222 includes a first fixing member 2221 and a second fixing member 2222. The first fixing member 2221 and the second fixing member 2222 extend radially of the clamping means 221 so as to be spaced along the circumferential direction of the clamping means 221. By installing the first fixing member 2221 and the second fixing member 2222, the clamping means 221 is fixed in at least two places.

[0077] Referring to Figures 12 and 15, the first fixing member 2221 and the second fixing member 2222 are installed at intervals along the circumferential direction of the second clamping body portion 2211b of the clamping body 2211. Specifically, when fixing the clamping means 221, one end of the first fixing member 2221 in the radial direction of the second clamping body portion 2211b abuts against the tapered surface of the fifth clamping drive unit 2213c, and one end of the second fixing member 2222 in the radial direction of the fifth clamping drive unit 2213c also abuts against the tapered surface of the fifth clamping drive unit 2213c.

[0078] Furthermore, the first fixing member 2221 and the second fixing member 2222 are both configured as fine adjustment members set in the micrometer structure and are used for fixing and fine adjustment of the clamping means 221, enabling the suction / discharge member 21 to be positioned in a predetermined location. Typically, the micrometer structure includes a spindle, sleeve, thimble, and ratchet stop. When fixing, the clamping means 221 is fixed by tightening the first fixing member 2221 and the second fixing member 2222. When fine adjustment, the ratchet stop and thimble of the first fixing member 2221 and the second fixing member 2222 are rotated until the suction / discharge member 21 is positioned in a predetermined location.

[0079] In some embodiments, the fixing means 222 further includes a third fixing member 2223. The third fixing member 2223 is positioned along the circumferential direction of the clamping means 221 and extends radially to the clamping means 221. By positioning the third fixing member 2223, the clamping means 221 is supported and fixed at least three points.

[0080] In some embodiments, the fixing means includes an elastic eccentric pressing element 2224, one end of which is connected to a third fixing member 2223, for eccentrically pressing the third fixing member 2223. The third fixing member 2223 and the elastic eccentric pressing element 2224 can employ a combination of a spring seat and a spring, etc. By contacting the clamping means 221, the third fixing member 2223 moves the first fixing member 2221 or the second fixing member 2222 against the eccentric pressing force of the elastic eccentric pressing element 2224, thereby adjusting the position of the clamping means 221. When fixed, the third fixing member 2223 and the elastic eccentric pressing element 2224 elastically support the clamping means 221, thereby ensuring the mounting stability of the clamping means 221. Furthermore, the third fixing member 2223 is installed along the first direction S1, and the first fixing member 2221 and the second fixing member 2222 are installed symmetrically on both sides of the third fixing member 2223 with respect to the first direction S1. It should be understood that the number and type of fixing means 222 can be set according to actual requirements and are not limited thereto, as long as the suction discharge member 21 can be fixed.

[0081] Referring to Figures 17 and 18, schematic diagrams of the housing member in several embodiments of the present application are shown. Referring to Figures 8 to 10, the housing member 223 has a hollow structure and is used to house at least a portion of the clamping means 221. A first opening 2231 and a second opening 2232 are provided at both end faces of the housing member 223 in the third direction S3. The clamping means 221 is positioned by passing sequentially through the first opening 2231 and the second opening 2232 along the third direction S3. The clamping means 221 is removed by exiting sequentially through the second opening 2232 and the first opening 2231. The dimensions of the first opening 2231 are formed to be larger than the dimensions of the clamping means 221, and the position of the clamping means 221 relative to the first opening 2231 may be adjusted by moving the first fixing member 2221, or by moving the second fixing member 2222.

[0082] Referring to Figure 15, the dimensions of the first opening 2231 are smaller than the outer diameter of the first clamping body portion 2211a and larger than the outer diameter of the second clamping body portion 2211b, that is, at least a portion of the clamping body 2211 protrudes from the first opening 2231 in the third direction S3. Referring to Figure 13, the diameter of the second opening 2232 is smaller than the outer diameter of the operating portion 2212b and larger than the outer diameter of the second clamping drive portion 2212c, thereby enabling the insertion of the suction / discharge member 21 and the first clamping drive member 2212. The clamping means 221 is attached to and housed in the housing member 223 through the first opening 2231 and the second opening 2232.

[0083] The housing member 223 is provided with a first mounting hole 2233, a second mounting hole 2234, and a third mounting hole 2235 for attaching the fixing means 222. The first mounting hole 2233, the second mounting hole 2234, and the third mounting hole 2235 are all positioned along the radial direction of the clamping means 221. Specifically, referring to Figure 10, the first mounting hole 2233, the second mounting hole 2234, and the third mounting hole 2235 are positioned radially along the tapered surface of the second clamping body portion 2211b. At least a portion of the first fixing member 2221 passes through the first mounting hole 2233 and enters the first opening 2231, and contacts the clamping means 221. At least a portion of the second fixing member 2222 passes through the second mounting hole 2234 and enters the first opening 2231, contacting the clamping means 221. At least a portion of the third fixing member 2223 passes through the third mounting hole 2235 and enters the first opening 2231, contacting the clamping means 221. The elastic eccentric pressing element 2224 is located within the third mounting hole 2235 and eccentrically presses the third fixing member 2223 toward the center of the first opening 2231.

[0084] To secure the first fixing member 2221 and the second fixing member 2222, the housing member 223 is further provided with a first fixing hole 2236 and a second fixing hole 2237. The fastening member secures the first fixing member 2221 to the housing member 223 via the first fixing hole 2236 and the second fixing member 2222 to the housing member 223 via the second fixing hole 2237. Referring to Figure 18, the first fixing hole 2236 and the second fixing hole 2237 are installed in the housing member 223 at intervals along the second direction S2. The first fixing member 2221 and the second fixing member 2222 are secured to the housing member 223 by screwing the fastening member into the fixing holes and bringing it into contact with the first fixing member 2221 and the second fixing member 2222. Note that the structure of the housing member 223 is adjustable according to actual requirements and is not limited to the structure described above.

[0085] When attaching the clamping means 221, the first fixing member 2221 and the second fixing member 2222 are loosened, the clamping means 221 is removed from the housing member 223 along the third direction S3, the suction discharge member 21 is placed on the clamping means 221, and the placement of the suction discharge member 21 on the clamping means 221 is repeated until the suction discharge member 21 is fixed and attached to the clamping means 221. After that, the clamping means 221 with the suction discharge member 21 placed on it is inserted into the housing member 223 along the third direction S3, the first fixing member 2221 and the second fixing member 2222 are tightened, and the clamping means 221 is attached against the eccentric pressing force applied to the clamping means by the third fixing member 2223 and the elastic eccentric pressing element 2224.

[0086] The fixing means 222 may include one of the fixing members described above. In another embodiment, referring to Figure 36, the fixing means 222 includes a third fixing member 2223 and an elastic eccentric pressing element 2224. One end of the elastic eccentric pressing element 2224 is connected to the third fixing member 2223, and the third fixing member 2223 abuts against the clamping means 221, thereby fixing the clamping means 221 to the housing member 223 against the eccentric pressing force of the elastic eccentric pressing element 2224, and attaching and fixing the clamping means 221.

[0087] Referring again to Figures 4 to 6, in some embodiments, the selection movement assembly 23 is used to drive the suction / discharge member 21 to move along a third direction S3 relative to the main body 10 to achieve cell aspiration and discharge. The selection movement assembly 23 includes a selection drive member 231, a selection transmission member 232, and a selection movement member 233. Under the control of the control mechanism 60, the selection drive member 231 drives the selection transmission member 232 to rotate, thereby moving the selection movement member 233 in the third direction S3. Referring again to Figure 8, the housing member 223 is connected to the selection movement member 233 along a first direction S1, and the selection movement member 233 moves the suction / discharge member 21 along the third direction S3 by the driving of the selection drive member 231 and the rotation of the selection transmission member 232. In a specific embodiment, the selection drive member 231 employs a stepping motor to achieve quantitative movement of the suction / discharge member 21 and ensure the movement accuracy of the suction / discharge member 21. In this embodiment, the displacement accuracy of the suction / discharge member 21 is 2 μm. The selected transmission member 232 is configured as a ball screw extending in the third direction S3, and moves the suction / discharge member 21 in the third direction S3.

[0088] The housing member 223 is detachably connected to the selection-moving member 233 by a fastening member. To facilitate the attachment of the housing member 223, a coupling groove is provided on the selection-moving member 233. The coupling groove facilitates the positioning and attachment of the housing member 223 to the selection-moving member 233. It should be understood that the method of connecting the selection-moving member 233 and the mounting assembly 22 is not limited, as long as the selection-moving assembly 23 can move the mounting assembly 22.

[0089] In some embodiments, the selection movement assembly 23 includes a selection guide member 234 and a selection sliding member 235. The selection guide member 234 extends in a third direction S3, and the selection sliding member 235 is installed on the selection guide member 234 and connected to the selection movement member 233, thereby enabling the movement of the selection movement member 233 in the third direction S3. The installation of the selection guide member 234 and the selection sliding member 235 facilitates the movement of the selection movement member 233. The extending lengths of the selection guide member 234 and the selection transmission member 232 are determined according to the movement stroke of the suction member and are not limited thereto.

[0090] In some embodiments, the selection movement assembly 23 further includes a selection displacement sensor 236 and a selection position limiting member 237. The selection displacement sensor 236 is installed on one side of the selection guide member 234 in the second direction S2. By installing the selection displacement sensor 236, the movement distance of the suction / discharge member 21 can be easily obtained, which contributes to the control of the movement of the suction / discharge member 21 by the control mechanism 60. The type and mounting position of the selection displacement sensor 236 are not limited as long as the transmission of the displacement signal can be achieved.

[0091] The selection position limiting member 237 extends in the third direction S3 and is connected to the selection movement member 233, and is installed on one side of the selection guide member 234 in the second direction S2. The selection position limiting member 237 and the selection displacement sensor 236 may be installed on the same side of the selection guide member 234, or on both sides. By installing the selection position limiting member 237, the movement distance of the suction / discharge member 21 in the third direction S3 is limited, the uppermost and lowermost positions of the suction / discharge member 21 can be obtained by the selection displacement sensor 236, and the suction / discharge member 21 can be prevented from colliding with the mounting mechanism 30 and being damaged during movement.

[0092] Under the control of the control mechanism 60, the selection drive member 231 drives the selection moving member 233 to move along the selection guide member 234 in a third direction S3, and moves the suction / discharge member 21 connected to the selection moving member 233 in the third direction S3. This enables the suction / discharge member 21 to approach and move away from the cells to be sorted, as well as the suction and discharge of the suction / discharge member 21 to the cells to be sorted.

[0093] Referring again to Figures 8 and 9, the selection illumination means 24 is connected to the mounting assembly 22 and is located at the end of the suction discharge member 21 away from the mounting mechanism 30. The selection illumination means 24 includes a selection light source 241 connected to the control mechanism 60. By positioning the selection light source 241 and the suction discharge member 21 along the third direction S3, the light emitted from the selection light source 241 can pass through the hollow conduit 211 of the suction discharge member 21, forming a projection of the suction discharge member 21, thereby facilitating the positioning of the suction discharge member 21. Referring again to Figure 15, the selection light source 241 is installed in the first housing chamber 2211c of the clamping body 2211.

[0094] To ensure the aspiration and release of cells by the suction / release member 21, it is necessary to seal the connection between the selection light source 241 and the first containment chamber 2211c, thereby isolating the first containment chamber 2211c from the external environment. In one embodiment, the selection light source 241 is fixed by adhesive within the first containment chamber 2211c of the first clamping body 2211a. The adhesive connection is easy to operate, ensures airtightness between the selection light source 241 and the clamping body 2211, and allows the suction / release member 21 to aspirate cells by the drive of the recovery assembly 26. It should be understood that the method of connecting the selection light source 241 and the clamping body 2211 is not limited, as long as the fixing of the selection light source 241 can be achieved.

[0095] In some embodiments, the selected light source 241 is an LED light source fixedly connected to the clamping body 2211, and the central axis of the LED light source is parallel to or coincides with the central axis of the suction / discharge member 21, so that the light emitted from the LED light source can pass through the hollow conduit 211 of the suction / discharge member 21. LED light sources have high illumination efficiency, a long service life, and are easy to install, thus enabling long-term use of the cell sorting device 100. The position of the selected light source 241 is set according to the position of the suction / discharge member 21, and the type of selected light source 241 is not limited to an LED light source as long as it is capable of emitting light.

[0096] In one embodiment, a seal is further installed between the selected light source 241 and the first clamping body 2211a. The seal is necessary to allow light to pass through the hollow conduit 211 of the suction / discharge member 21. By installing the seal, a buffer is provided to the selected light source 241, and the airtightness between the suction / discharge member 21 and the clamping body 2211 is further ensured.

[0097] In some embodiments, as shown in Figure 37, the cell sorting device 100 includes a suction / discharge member projection identification unit for identifying the projection of the suction / discharge member and forming a suction / discharge member projection mapping. In the suction / discharge member projection mapping, projection markers are placed. The first fixing member 2221 and the second fixing member 2223 can be used to adjust the projection of the suction / discharge member, change the position of the suction / discharge member projection mapping relative to the projection markers, position the projection markers at predetermined positions in the suction / discharge member projection mapping, and complete the localization of the suction / discharge member 21.

[0098] During use, the control mechanism 60 controls the selected light source 241 to emit light, causing the light to pass through the hollow conduit 211 of the suction / discharge member 21 and be emitted from the suction / discharge member 21 through the suction / discharge port 212. Based on the light emitted from the suction / discharge port 212, the projection of the suction / discharge member is acquired, and the imaging mechanism 40 acquires image information (e.g., a circular spot) of the projection of the suction / discharge member. Based on this image information and the projection marker (e.g., a crosshair) within it, the position of the crosshair in the circular spot is adjusted by moving the first fixing member 2221 or the second fixing member 2223, for example, moving the crosshair to the center of the circular spot. After marking the position of the suction / discharge member projection, the control mechanism 60 controls the selected moving assembly 23 based on this position to move the suction / discharge member 21 in a third direction S3, thereby achieving cell aspiration that meets predetermined requirements.

[0099] Referring again to Figures 4 and 5, the cleaning assembly 25 is used to clean the suction discharge member 21 before use, ensuring cell activity during the suction and discharge process so that the normal state of the cells is not disrupted. The cleaning assembly 25 includes a drive means 251 and a cleaning fluid means 254. The drive means 251 is connected to the hollow conduit 211 of the suction discharge member 21 via a connecting member 252, thereby changing the air pressure in the hollow conduit 211 of the suction discharge member 21 and allowing the cleaning fluid to enter the hollow conduit 211 of the suction discharge member 21 to clean the suction discharge member 21. Referring again to Figure 2, the drive means 251 is installed on the support plate 1221 along a first direction S1. To facilitate the suction of the cleaning fluid, the drive means 251 is connected to the suction discharge member 21 along a third direction S3.

[0100] In some embodiments, the driving means 251 employs a pump, which uses a mechanical device to reciprocate a diaphragm inside the pump, compressing or expanding the air in the pump chamber to generate negative pressure. This creates a pressure difference between the suction outlet 212 of the suction discharge member 21 and the external atmospheric pressure, and this pressure difference draws the cleaning liquid into the suction discharge member 21. Referring to Figures 4, 12, and 15, the driving means 251 is connected to the connection hole of the clamping body 2211, and the clamping body 2211 and the suction discharge member 21 form a cleaning chamber for cleaning the hollow conduit 211 of the suction discharge member 21.

[0101] In some embodiments, referring to Figures 4 and 5, the cleaning assembly 25 further includes a communicating member 252 to facilitate connection between the drive means 251 and the clamping body 2211. The communicating member 252 is connected at one end to the drive means 251 and at the other end to a connection hole in the clamping body 2211, thereby forming a communication passage with the hollow conduit 211 of the suction discharge member 21, and thereby generating a pressure difference at the suction discharge port 212. Specifically, the communicating member 252 can be a plastic hose.

[0102] Furthermore, to facilitate the connection between the clamping body 2211 and the communicating member 252, the cleaning assembly 25 further includes a passage connecting member 253. One end of the passage connecting member 253 is connected to the clamping body 2211 via a connecting hole, and the other end is covered by the communicating member 252. The passage connecting member 253 may employ a valve connector, one end of which is screwed into the clamping body 2211, the other end of which is covered by the communicating member 252, and which has good sealing performance between itself and the communicating member 252.

[0103] The passage connecting member 253 has a hollow structure and forms a connecting passage together with the communication member 252 and the suction / discharge member 21. During cleaning, the cleaning fluid flows through the inner wall of the suction / discharge member 21, the inner wall of the clamping body 2211, and the inner wall of the passage connecting member 253 to perform cleaning. The passage connecting member 253 and the clamping body 2211 are screwed together. It should be understood that the method of connecting the passage connecting member 253 and the clamping body 2211 is not limited as long as the connection between them can be achieved, and for example, the passage connecting member 253 and the clamping body 2211 may be integrally molded.

[0104] In some embodiments, to facilitate the storage of the washing solution, the washing assembly 25 further includes a storage means 255 for storing the washing solution means 254. The storage means 255 is located on the mounting mechanism 30 of the cell sorting device 100. The washing solution means 254 includes a first washing solution 2541 and a second washing solution 2542, which are different from each other. The storage means 255 includes a first storage means 2551 for storing the first washing solution 2541 and a second storage means 2552 for storing the second washing solution 2542. The drive means 251 washes the suction discharge member 21 a first predetermined number of times by drawing the first washing solution 2541 into the hollow conduit 211, and washes the suction discharge member 21 a second predetermined number of times by drawing the second washing solution 2542 into the hollow conduit 211.

[0105] In specific examples, ethanol is used as the first washing solution 2541, phosphate-buffered saline (PBS) is used as the second washing solution 2542, and culture dishes are used as both the first and second storage means 2551 and 2552. Ethanol is used to sterilize and disinfect the suction / discharge member 21, and the PBS buffer is used to provide an internal environment suitable for cell culture, so that the inside of the suction / discharge member 21 does not affect cell activity. It should be understood that the type of washing solution is not limited as long as the washing of the suction / discharge member 21 can be achieved, and for example, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer may be used as the second washing solution 2542.

[0106] Furthermore, 75% ethanol is used as the first washing solution 2541, and 0.01 M PBS buffer is used as the second washing solution 2542. 75% ethanol is effective in killing bacteria and viruses. Alternatively, the first washing solution 2541 and the second washing solution 2542 may be prepared to obtain desired concentrations, or they may be used directly. Therefore, the concentration of the washing solution can be set according to actual requirements and is not limited to the concentrations described above.

[0107] To selectively ensure a sterile environment in the cell sorting process, the first washing solution 2541 and the second washing solution 2542 must be filtered before use. Specifically, both ethanol and PBS buffer are used after being filtered to 0.45 μm. The filtration size requirement can be set according to the requirements of the experiment and is not limited to 0.45 μm; for example, filtration to 0.22 μm is also possible. In one embodiment, the storage means assembly and the washing solution assembly are sterilized, for example, by a sterilization kettle before washing. The specific sterilization method is not limited as long as the sterilization and disinfection of the storage means and washing solution can be achieved.

[0108] Before the suction discharge member 21 aspirates cells, the drive means 251 is connected to the suction discharge member 21, and an appropriate amount of the first washing solution 2541 is added to the first storage means 2551, and the first storage means 2551 with the first washing solution 2541 added is positioned at a distance from the suction discharge member 21 along the third direction S3. Under the control of the control mechanism 60, the selection movement assembly 23 is driven to move the suction discharge port 212 of the suction discharge member 21 into the first washing solution 2541, and the drive means 251 drives the suction discharge member 21 to wash the suction discharge member 21 by drawing the first washing solution 2541 into the hollow conduit 211 of the suction discharge member 21, and drives the suction discharge member 21 to discharge the first washing solution 2541 into the first storage means 2551. The suction and discharge operation for the first cleaning solution 2541 is performed once or multiple times, and the specific number of times is determined to be a predetermined number of times to complete the primary cleaning and to perform disinfection and sterilization on the hollow conduit 211 of the suction and discharge member 21.

[0109] Next, an appropriate amount of the second cleaning solution 2542 is added to the second storage means 2552, and the second storage means 2552 with the added cleaning solution 2542 is positioned at a distance from the suction discharge member 21 along the third direction S3. Under the control of the control mechanism 60, the selection movement assembly 23 is driven to move the suction discharge port 212 of the suction discharge member 21 into the second cleaning solution 2542, and the drive means 251 drives the suction discharge member 21 to draw the second cleaning solution 2542 into the hollow conduit 211 of the suction discharge member 21 to clean the suction discharge member 21, and drives the suction discharge member 21 to discharge the second cleaning solution 2542 into the second storage means 2552. The suction and discharge operation for the second cleaning solution 2542 is performed once or multiple times, and the specific number of times is determined to be a predetermined number to complete the secondary cleaning. The secondary washing process cleans the first washing solution 2541 while providing a stable ionic environment and pH buffering function in the suction / discharge member 21 that contributes to cell survival.

[0110] The steps after cell aspiration by the suction discharge member 21 are the reverse of the steps before cell aspiration by the suction discharge member. Specifically, an appropriate amount of the second washing solution 2542 is added to the second storage means 2552, and the second storage means 2552 with the second washing solution 2542 added is positioned at a distance from the suction discharge member 21 along the third direction S3. Under the control of the control mechanism 60, the selection movement assembly 23 is driven to move the suction discharge port 212 of the suction discharge member 21 into the second washing solution 2542, and the drive means 251 drives the suction discharge member 21 to wash the suction discharge member 21 by drawing the second washing solution 2542 into the hollow conduit 211 of the suction discharge member 21, and drives the suction discharge member 21 to discharge the second washing solution 2542 into the second storage means 2552. The suction and discharge operation for the second washing solution 2542 is performed once or multiple times, and the specific number of times is determined to be a predetermined number to complete the primary washing and wash away any cells or cell buffer remaining in the suction / discharge member 21.

[0111] Next, an appropriate amount of the first cleaning solution 2541 is added to the first storage means 2551, and the first storage means 2551 with the first cleaning solution 2541 added is positioned at a distance from the suction discharge member 21 along the third direction S3. Under the control of the control mechanism 60, the selection movement assembly 23 is driven to move the suction discharge port 212 of the suction discharge member 21 into the first cleaning solution 2541, and the drive means 251 drives the suction discharge member 21 to draw the first cleaning solution 2541 into the hollow conduit 211 of the suction discharge member 21 to clean the suction discharge member 21, and drives the suction discharge member 21 to discharge the first cleaning solution 2541 into the first storage means 2551. The suction and discharge operation of the first cleaning solution 2541 is performed once or multiple times, and the specific number of times is determined to be a predetermined number to complete the secondary cleaning. Therefore, the hollow conduit 211 of the suction discharge member 21 is disinfected and sterilized while the second cleaning solution 2542 is being used to clean it.

[0112] In some embodiments, the control mechanism 60 includes a cleaning control means 61. The cleaning control means 61 controls the drive means 251 to draw a first cleaning liquid 2541 into the hollow conduit 211 and clean the suction discharge member 21 a first predetermined number of times, and controls the drive means 252 to draw a second cleaning liquid 2542 into the hollow conduit 211 and clean the suction discharge member 21 a second predetermined number of times. The cleaning control means 61 may control the drive means 251 to clean the drawn first cleaning liquid 2541 by moving it back and forth multiple times along a third direction S3 in the hollow conduit 211 before releasing it into the first storage means 2551, and may also control the drive means 251 to clean the drawn second cleaning liquid 2542 by moving it back and forth multiple times along a third direction S3 in the hollow conduit 211 before releasing it into the second storage means 2552.

[0113] Referring again to Figures 4 and 5, the recovery assembly 26 is used for aspirating and releasing cells that meet predetermined requirements. The recovery assembly 26 includes a drive means 251, a communication member 252, and a passage connecting member 253. For specific operational and positional relationships, please refer to the description of the washing assembly 25, and the description will be omitted here. By installing the recovery assembly 26, cell aspiration and release are achieved under the control of the control mechanism 60, facilitating the cell sorting process and improving the efficiency of cell sorting.

[0114] When the recovery assembly 26 is used, cells that meet a predetermined requirement are moved so that they are spaced apart from the suction discharge member 21 along the third direction S3. Under the control of the control mechanism 60, the selection and movement assembly 23 is driven to move the suction discharge port 212 of the suction discharge member 21 to a predetermined position. At this time, the suction discharge port 212 and the cells that meet the predetermined requirement are separated by a predetermined distance, and the control mechanism 60 controls the drive means 251 to generate a pressure difference at the suction discharge port 212 of the suction discharge member 21, thereby drawing the cells that meet the predetermined requirement into the hollow conduit 211 and completing the suction of the cells that meet the predetermined requirement. The predetermined distance is set so that the suction discharge member 21 can draw the cells that meet the predetermined requirement into the hollow conduit 211.

[0115] To facilitate the installation of the selection movement assembly 23, referring to Figure 4, the selection mechanism 20 further includes a mounting bracket 27. The installation of the mounting bracket 27 enables the fixed mounting of the selection movement assembly 23. The selection mechanism 20 is attached to the main body 10 by the mounting bracket 27. In some embodiments, the mounting bracket 27 is connected to the imaging mechanism 40 along a third direction S3, and the imaging mechanism 40 is attached to the main body 10. The mounting bracket 27 can be installed according to actual requirements, for example, it can be connected to a support plate 1221 along a first direction S1 so that the selection mechanism 20 is attached to the main body 10. In some embodiments, the mounting bracket 27 is provided with a first mounting seat 271 and a second mounting seat 272. The first mounting seat 271 is installed on the mounting bracket 27 along the third direction S3 and is used for the fixed mounting of the selection guide member 234. The second mounting seat 272 is installed on the mounting bracket 27 along the first direction S1 so as to be perpendicular to the first mounting seat 271, and is used for positioning and fixing the selected drive member 231.

[0116] When using the selection mechanism 20, the mounting assembly 22 and the selection and movement assembly 23 are connected, and the suction and discharge member 21 is fixedly attached to the mounting assembly 22. The selection and movement assembly 23 is driven to move the suction and discharge member 21, and the cleaning assembly 25 completes the cleaning of the suction and discharge member 21. Subsequently, the selection and movement assembly 23 is driven to move the suction and discharge member 21, and the recovery assembly 26 causes the suction and discharge member 21 to aspirate and recover cells that meet the predetermined requirements at a predetermined position. After the aspiration is complete, the selection and movement assembly 23 is driven to move the suction and discharge member 21, and the cleaning assembly 25 completes the cleaning of the suction and discharge member 21.

[0117] In some embodiments, the mounting mechanism in some embodiments of the present application is shown with reference to Figures 19 and 20. The mounting mechanism 30 is used to mount and place the sorting target member containing the sorting target cells. Referring to Figure 2, the mounting mechanism 30 is installed at a distance from the selection mechanism 20 along the third direction S3 and is positioned between the imaging mechanism 40 and the selection mechanism 20.

[0118] The mounting mechanism 30 is configured to be attached to the main body 10. In some embodiments, the mounting mechanism 30 is directly attached to the main body 10 by being connected to a support seat 122. In some embodiments, the mounting mechanism 30 is indirectly attached to the main body 10 by being connected to an imaging mechanism attached to the main body 10.

[0119] The mounting mechanism 30 can move the member to be sorted to a predetermined position so that cells that meet predetermined requirements can be aspirated and collected by the selection mechanism 20. The mounting mechanism 30 includes a mounting assembly 31 and a mounting and moving assembly 32. The mounting assembly 31 is used to mount the member to be sorted and is positioned on the mounting and moving assembly 32. The mounting and moving assembly 32 can be driven to move the mounting assembly 31 so that cells that meet predetermined requirements within the member to be sorted are positioned at a distance from the aspiration and discharge member 21 along the third direction S3. By installing the mounting mechanism 30, the mounting and moving of the member to be sorted is realized, enabling the aspiration and collection of cells that meet predetermined requirements, and improving the efficiency of cell sorting.

[0120] In some embodiments, with reference to Figure 21, the mounting assembly of the mounting mechanism 30 in some embodiments of the present application is shown. The mounting assembly 31 includes a first mounting member 311 and a second mounting member 312. The first mounting member 311 and the second mounting member 312 are spaced apart along a third direction S3. The first mounting member 311 is movably connected to the second mounting member 312 and moves along the first direction S1 relative to the second mounting member 312. The second mounting member 312 moves together with the first mounting member 311 along the second direction S2.

[0121] The first mounting member 311 is on which the sorting target member containing the cells to be sorted is placed. The first mounting member 311 can be moved along a first direction S1 relative to the base 121 of the main body 10 by the drive of the mounting and moving assembly 32. The second mounting member 312 is on which the first mounting member 311 is placed. The second mounting member 312 can be moved along a second direction S2 relative to the base 121 of the main body 10 by the drive of the mounting and moving assembly 32, thereby moving the sorting target member to a predetermined area relative to the base 121 of the main body 10, thereby enabling the aspiration and retrieval of cells that meet the predetermined requirements of the sorting target member in the predetermined area. It should be understood that the direction of movement of the first mounting member 311 and the second mounting member 312 can be set according to the actual requirements and are not limited thereto. For example, the first mounting member 311 can be moved along the second direction S2 by driving the mounting and moving assembly 32, and the second mounting member 312 can be moved along the first direction S1 by driving the mounting and moving assembly 32.

[0122] Referring to Figures 2 and 22, the first mounting member in several embodiments of the present application is shown. The first mounting member 311 is equipped with mounting sections 3111. The mounting sections 3111 allow the members to be sorted to be placed on them, and the imaging mechanism 40 can form an image on the cells to be sorted placed on the members to be sorted to acquire image information of the cells to be sorted. In order to make full use of the space of the first mounting member 311 and improve the sorting efficiency of the cells to be sorted, a predetermined number of mounting sections 3111 are installed on the first mounting member 311, and a predetermined number of members to be sorted are placed on it. For example, the first mounting member 311 has three mounting sections 3111 arranged at intervals along the second direction S2, enabling the sorting of cells to be sorted placed on three members to be sorted.

[0123] In some embodiments, referring to Figure 21, the mounting assembly 31 further includes a third mounting member 313. The third mounting member 313 is on which the first mounting member 311 and the second mounting member 312 are mounted, and is positioned at a distance from the second mounting member 312 along a third direction S3. The second mounting member 312 moves along a second direction S2 relative to the third mounting member 313.

[0124] In some embodiments, referring to Figure 21, the mounting assembly 31 further includes a storage means 314 installed on the first mounting member 311. The storage means 314 is used to store and store cells that meet a predetermined requirement so as to culture cells that meet a predetermined requirement. Referring to Figure 2, cells that meet a predetermined requirement are drawn into the selection mechanism 20 and placed on the storage means 314. The storage means 314 is then transferred to a culture vessel to culture the cells that meet the predetermined requirement.

[0125] The storage means 314 has a porous structure as a whole. By installing a porous structure, multiple cells can be collected and placed, improving the efficiency of cell sorting and increasing the success rate and accuracy of cell sorting. For example, the storage means 314 has 96 holes. The number of holes in the storage means 314 can be set according to the actual requirements and is not limited thereto.

[0126] Referring to Figure 25, which shows a storage means in several embodiments of the present application, the storage means 314 includes a support frame 3141 and reaction tubes 3142. The support frame 3141 is reusable. The reaction tubes 3142 are arranged on the support frame 3141 at intervals along a second direction S2 and can be independently recovered and cultured. Selectively, the distances between the reaction tubes 3142 are the same. For example, eight reaction tubes 3142 are installed at equal intervals along a first direction S1 on the support frame 3141, and twelve reaction tubes 3142 are installed at equal intervals along a second direction S2. The material of the storage means 314 is not limited as long as cell recovery and culture can be achieved, and various materials can be used according to actual requirements. For example, the materials of the support frame 3141 and the storage unit 3142a assembly may be the same or different.

[0127] The support frame 3141 has through holes, and the reaction tube 3142 is connected to the support frame 3141 through these holes. For the placement of the support frame 3141 and the positioning of the storage section 3142a assembly, one corner of the support frame 3141 is set as a positioning chamfer, and the other corners are all set as rounded corners. By setting rounded corners, it is possible to prevent the corners of the support frame 3141 from becoming sharp, which contributes to recovery and cultivation by the storage means 314. The support frame 3141 is fitted with a skirt structure that extends in a third direction S3 in the circumferential direction. By fitting the skirt structure, the structural strength of the support frame 3141 itself can be strengthened and twisting deformation of the support frame 3141 can be prevented. Furthermore, the skirt structure is configured in a stepped shape to facilitate the placement of the support frame 3141.

[0128] Referring to Figure 26, which shows schematic diagrams of reaction tubes 3142 in several embodiments of the present application, the reaction tube 3142 includes a storage section 3142a and a mounting platform 3142b for mounting the storage section 3142a. The mounting platform 3142b is provided with mounting holes corresponding to the storage section 3142a. By installing the storage section 3142a, a place is provided for housing and culturing cells that meet predetermined requirements. Referring to Figure 24, the mounting holes in the mounting platform 3142b and the through holes in the support frame 3141 all correspond one-to-one with the storage sections 3142a. The number of storage sections 3142a is equal to the number of through holes.

[0129] The storage section 3142a has one end extending in a third direction S3, and at least a portion of the tubular body's dimensions gradually increase along the third direction S3, forming a cone shape, with the other end configured as an opening into which cells meeting a predetermined requirement are placed. The cone shape facilitates cell retrieval. In some embodiments, the storage section 3142a is transparent, allowing observation of cells meeting a predetermined requirement in subsequent processes.

[0130] The mounting base portion 3142b is a rectangular thin plate, and positioning holes are provided at both ends in the first direction S1, not located on the same axis. This arrangement facilitates the positioning of different storage portions 3142a. In some embodiments, both the mounting base portion 3142b and the storage portion 3142a are formed by injection molding, and then the reaction tube 3142 is formed by heat bonding.

[0131] In a specific embodiment, each reaction tube 3142 includes a predetermined number of storage compartments 3142a installed in the first direction S1. The number of reaction tubes 3142 to be installed can be determined according to the specific usage requirements, thereby contributing to improved flexibility in the use of the storage means 314, as well as further reduction of consumables and avoidance of waste. For example, if the support frame 3141 has 96 mounting through holes and 32 storage compartments 3142a are required in a certain experiment, only four reaction tubes 3142 can be installed on the support frame 3141. The predetermined number of storage compartments 3142a in each reaction tube 3142 may be set in other arrangement configurations, such as an array arrangement or a rectangular grid arrangement of multiple storage compartments 3142a.

[0132] Referring to Figures 19 to 22, the first mounting member 311 is provided with a mounting groove 3112 for mounting the storage means 314. The storage means 314 is placed on the first mounting member 311 via the mounting groove 3112, thereby achieving the mounting and fixing of the storage means 314. Specifically, the storage means 314 is placed on the first mounting member 311 through the cooperation of the stepped skirt structure of the support frame 3141 and the mounting groove 3112. The shape and dimensions of the mounting groove 3112 are compatible with the shape and dimensions of the support frame 3141. Since the storage means 314 and the members to be sorted are installed at intervals along the first direction S1, the mounting groove 3112 and mounting portion 3111 of the first mounting member 311 are also installed at intervals along the first direction S1 to correspond to this. As long as the aspiration and collection of cells that meet the predetermined requirements can be achieved, the positions of the sorting target members and the storage means 314 are not limited. For example, the sorting target members and the storage means 314 may be arranged at intervals along the second direction S2, or the storage means 314 may be arranged at intervals along the circumferential direction of the sorting target members.

[0133] Furthermore, to better secure the first mounting member 311, an elastic buffer member 3113 is further installed on the first mounting member 311. The elastic buffer member 3113 is installed at least one corner of the mounting groove 3112. The storage means 314 is fixed to the first mounting member 311 by the elastic buffer member 3113. Referring to Figures 25 and 26, in order to prevent the storage section 3142a from contacting the first mounting member 311 and affecting the collection of cells that meet predetermined requirements, the first mounting member 311 is provided with a through hole communicating with the mounting groove 3112. The through hole has a smaller dimension than the mounting groove 3112, and a step is formed between it and the mounting groove 3112, allowing the storage means 314 to be placed. The size of the through hole is not limited as long as the proper placement of the storage section 3142a can be guaranteed.

[0134] Referring again to Figures 19 and 20, the mounting and moving assembly 32 can be driven to move the mounting assembly 31 so aspirate cells that meet predetermined requirements within the member to be sorted, and then move the storage means 314 so aspirate cells that meet predetermined requirements are released into the storage means 314. Referring to Figure 2, the control mechanism 60 can control the movement of the mounting and moving assembly 32, as well as the aspiration and release by the selection mechanism 20, to achieve the aspiration and release of cells that meet predetermined requirements.

[0135] In some embodiments, the mounting and moving assembly 32 includes a first mounting and moving means 321 and a second mounting and moving means 322. The first mounting and moving means 321 drives the mounting assembly 31 to move along a first direction S1, and the second mounting and moving means 322 drives the mounting assembly 31 to move along a second direction S2. Specifically, the first mounting and moving means 321 drives the first mounting member 311 to move along the first direction S1, and the second mounting and moving means 322 drives the second mounting member 312 to move along the second direction S2.

[0136] In some embodiments, the first mounting and moving means 321 includes a first mounting drive member 3211, a first mounting transmission member 3212, and a first mounting and moving member 3213. The first mounting drive member 3211 can drive the first mounting transmission member 3212 to rotate, thereby enabling the movement of the first mounting and moving member 3213 in a first direction S1. The first mounting and moving member 3213 is connected to the first mounting member 311, and the first mounting transmission member 3212 extends in the first direction S1. The drive of the first mounting drive member 3211 causes the first mounting and moving member 3213 to move the first mounting member 311 in the first direction S1.

[0137] Specifically, the first mounting drive member 3211 employs a stepping motor to achieve quantitative movement of the first mounting member 311, thereby controlling the movement of the first mounting member 311, the sorting target member and storage means 314 installed on the first mounting member 311, by a predetermined distance in the first direction S1. It should be understood that the type of the first mounting drive member 3211 is not limited as long as movement along the first direction S1 of the mounting assembly 31 can be achieved.

[0138] Selectively, the first mounting drive member 3211 employs a 5-phase stepping motor. The 5-phase stepping motor enables highly accurate positioning of the first mounting member 311, and allows the static angle error, when controlled by a drive device using microstepping drive, to be maintained with nearly the same high precision as when using full-step drive. Compared with other stepping motors, the 5-phase stepping motor also achieves low vibration and low noise operation. The 5-phase stepping motor contributes to the highly accurate automatic movement of the mounting assembly 31.

[0139] In some embodiments, to facilitate the movement of the first mounting member 311 along a first direction S1, the first mounting moving means 321 further includes a first mounting guide member 3214. The first mounting guide member 3214 extends in the first direction S1, thereby moving the first mounting member 311 along the first direction S1. The first mounting guide member 3214 is positioned on both sides of the second mounting member 312 at intervals along a second direction S2. The spaced-out first mounting guide member 3214 ensures the stability of the movement of the first mounting member 311 along the first direction S1. The extending lengths of the first mounting transmission member 3212 and the first mounting guide member 3214 are determined by the movement stroke of the first mounting member 311 to be installed.

[0140] To facilitate the installation of the first mounting guide member 3214, a second mounting member and a first mounting moving means in some embodiments of the present application are shown with reference to Figure 23. The second mounting member 312 is provided with the first mounting guide groove 3121, and the first mounting guide member 3214 is positioned in the first mounting guide groove 3121 to ensure the stability of the first mounting member 311 during movement. It should be understood that the first mounting guide member 3214 may be positioned on the surface of the second mounting member 312.

[0141] In some embodiments, the first mounting and moving means 321 further includes a first mounting and sliding member 3215. The first mounting and sliding member 3215 is installed on a first mounting and guide member 3214 and connected to the first mounting and sliding member 311. The sliding of the first mounting and sliding member 3215 on the first mounting and guide member 3214 moves the first mounting and sliding member 311 along the first direction S1. By installing the first mounting and sliding member 3215, wear of the first mounting and sliding member 311 during movement is reduced, contributing to improved movement stability and displacement accuracy of the first mounting and sliding member 311. Furthermore, a predetermined number of first mounting and sliding members 3215 are installed for each first mounting and guide member 3214. The first mounting and sliding members 3215 are arranged on the first mounting and sliding member 3214 at intervals along the first direction S1. A predetermined number of first mounting and sliding members 3215 further ensure the stability of the first mounting member 311 during movement.

[0142] In some embodiments, the first mounting and moving means 321 further includes a displacement sensor (not shown). By installing the displacement sensor, positional information of the sorting target members and the storage means 314 can be acquired, and the control mechanism 60 can be controlled to automatically move the mounting assembly 31, thereby improving the efficiency of cell sorting. Specifically, a grating displacement sensor is employed as the displacement sensor. Grating displacement sensors have high measurement accuracy and resolution, excellent stability, and strong noise immunity, making them easy to interface with a computer and suitable for long-distance transmission. It should be understood that the type of displacement sensor is not limited as long as positional information of the mounting assembly 31 can be acquired.

[0143] In some embodiments, referring to Figures 19 and 20, the second mounting and moving means 322 includes a second mounting drive member 3221, a second mounting transmission member 3222, and a second mounting and moving member 3223. The second mounting drive member 3221 can drive the second mounting transmission member 3222 to rotate, thereby enabling the movement of the second mounting and moving member 3223 in a second direction S2. The second mounting and moving member 3223 is connected to the second mounting member 312, and the second mounting transmission member 3222 extends in the second direction S2. The driving of the second mounting drive member 3221 enables the movement of the second mounting member 312 along the second direction S2.

[0144] Specifically, the second mounting drive member 3221 employs a stepping motor and controls the movement of the sorting target member and storage means 314 by a predetermined distance in the second direction S2 by achieving quantitative movement of the second mounting member 312. It should be understood that the type of the second mounting drive member 3221 is not limited as long as movement along the second direction S2 of the mounting assembly 31 can be achieved. Selectively, the second mounting drive member 3221 also employs a 5-phase stepping motor. By installing a 5-phase stepping motor, it contributes to the high-precision automatic movement of the mounting assembly 31. The movement accuracy of the mounting assembly 31 needs to be set to satisfy the movement requirements of the sorting target member, i.e., that the aspiration of adjacent cells that meet predetermined requirements is not interfered with. For example, the movement accuracy of the mounting assembly 31 is set to 2 μm.

[0145] In some embodiments, the second mounting and moving means 322 further includes a second mounting guide member 3224. The second mounting guide member 3224 extends in a second direction S2 and is positioned at intervals along the first direction S1 on the third mounting member 313. The installation of the second mounting guide member 3224 facilitates the movement of the second mounting member 312. The extending lengths of the second mounting transmission member 3222 and the second mounting guide member 3224 are determined by the movement stroke of the second mounting member 312 on which it is installed.

[0146] Referring to Figure 24, a third mounting member and a second mounting movement means are shown in some embodiments of the present application. The third mounting member 313 is provided with a second mounting guide groove 3131, and the second mounting guide member 3224 is installed in the second mounting guide groove 3131, thereby ensuring the movement stability of the second mounting member 312. It should be understood that the second mounting guide member 3224 and the second mounting guide groove 3131 may be installed in reverse, that is, the second mounting guide member 3224 may be installed on the third mounting member 313 and the second mounting guide groove 3131 may be installed on the second mounting member.

[0147] In some embodiments, the second mounting and moving means 322 further includes a second mounting and sliding member 3225. The second mounting and sliding member 3225 is mounted on a second mounting and guide member 3224 and connected to the second mounting and sliding member 312. The sliding of the second mounting and sliding member 3225 on the second mounting and guide member 3224 moves the second mounting and sliding member 312, reducing wear of the second mounting and sliding member 312 during movement and improving the stability and positional accuracy of the second mounting and sliding member 312 during movement. Furthermore, a predetermined number of second mounting and sliding members 3225 are installed for each second mounting and guide member 3224. The second mounting and sliding members 3225 are arranged on the second mounting and sliding member 3224 at intervals along a first direction S1. A predetermined number of second mounting and sliding members 3225 further ensure the movement stability of the second mounting member 312.

[0148] In some embodiments, the second mounting and moving means 322 further includes a displacement sensor. The displacement sensor employs a proximity displacement sensor. Proximity displacement sensors have a simple structure, excellent stability and reliability, and high measurement accuracy and sensitivity. It should be understood that the type and mounting position of the displacement sensor are not limited as long as displacement information of the second mounting member 312 can be obtained.

[0149] Referring to Figures 23 and 24, the first mounting and moving means 321 is installed on one side of the second mounting member 312 in the second direction S2, and the second mounting and moving means 322 is installed on one side of the third mounting member 313 in the first direction S1. Note that the mounting positions of the first mounting and moving means 321 and the second mounting and moving means 322 are not limited, as long as the movement of the first mounting member 311 along the first direction S1 and the movement of the second mounting member 312 along the second direction S2 can be achieved.

[0150] Referring again to Figure 19, the mounting mechanism 30 further includes a mounting position limiting assembly 33. The mounting position limiting assembly 33 is installed on the mounting assembly 31 and is used to fix the members to be sorted to the mounting assembly 31. The mounting position limiting assembly 33 includes an elastic member 331 and an eccentric pressing connecting member 332. The elastic member 331 surrounds the surface of the members to be sorted and is configured to press radially eccentrically against the members to be sorted. Referring to Figure 20, the eccentric pressing connecting member 332 is positioned at intervals along the second direction S2 on the first mounting member 311, and the elastic member 331 covers the eccentric pressing connecting member 332. In a specific embodiment, the eccentric pressing connecting member 332 is installed along the circumferential direction of the mounting portion 3111 of the first mounting member 311, so that the elastic member 331 can eccentrically press and restrict the position of the member to be sorted placed on the first mounting member 311. After the member to be sorted is placed on the first mounting member 311, the elastic member 331 is pulled from one side of the mounting portion 3111 to the other side of the mounting portion 3111, eccentrically pressing the member to be sorted and bringing the member to be sorted into contact with the mounting portion 3111, thereby ensuring the stability of the member to be sorted during placement and subsequent movement.

[0151] In some embodiments, the mounting position limiting assembly 33 further includes a position limiting member 333. By installing the position limiting member 333, sorting members of different sizes can be fixed to the mounting section 3111. The shape and dimensions of the inner diameter of the position limiting member 333 substantially match the shape and dimensions of the outer diameter of the sorting member, so that the outer wall of the sorting member is in close contact with the inner wall of the position limiting member 333. The shape and dimensions of the outer diameter of the position limiting member 333 substantially match the shape and dimensions of the position limiting groove of the mounting section 3111, so that the outer wall of the position limiting member 333 is in close contact with the position limiting groove of the mounting section 3111, thereby achieving position limiting of the sorting member relative to the first mounting member 311.

[0152] In a specific embodiment, referring to Figure 22, the position limiting member 333 is made of silicone rubber. By installing the position limiting member 333, friction between the sorting target member and the mounting section 3111 is increased, making the sorting target member more stable. The elastic member 331 can eccentrically press the position limiting member, providing a cushioning effect to the sorting target member, avoiding or reducing damage to the sorting target member by the elastic member 331, and ensuring the normal use of the sorting target member. It should be understood that the material, shape, and dimensions of the position limiting member 333 can be set according to actual requirements and are not limited thereto.

[0153] In other embodiments, the position limiting member 333 may be a retaining member, and a predetermined number of retaining members are installed at intervals in the position limiting groove of the mounting section 3111. By installing the retaining members, the position of the members to be sorted relative to the mounting section 3111 is limited. Springs or spring pins can be used as retaining members. When the members to be sorted are placed on the mounting section 3111, the members to be sorted press against the retaining members of the mounting section 3111, and as the retaining members are compressed, pressure is applied to the members to be sorted, thereby fixing the members to the mounting section 3111. It should be understood that the number and position of the retaining members can be set according to actual requirements and are not limited thereto.

[0154] In some embodiments, the position limiting member 333 employs a fastening member. The member to be sorted is fixed to the mounting section 3111 by installing the fastening member on the inner wall of the mounting section 3111. The position limiting member may also be a clamping member. The member to be sorted is fixed to the mounting section 3111 by installing the clamping member on the peripheral edge side of the mounting section 3111. It should be understood that the method of connecting the position limiting member and the member to be sorted is not limited as long as the member to be sorted is fixed to the mounting section 3111.

[0155] During the operation of the placement mechanism 30, the members to be sorted are placed on the placement assembly 31, the position of the members to be sorted is restricted by the placement position limiting assembly 33, and the placement movement assembly 32 is driven to move the members to be sorted, thereby achieving the aspiration of cells that meet the predetermined requirements. Then, by driving the storage means 314 to move, the cells that meet the requirements are recovered and cultured.

[0156] Specifically, when aspirating cells that meet predetermined requirements, under the control of the control mechanism 60, the first mounting and moving means 321 drives the sorting target member to move in the first direction S1, and the second mounting and moving means 322 drives the sorting target member to move in the second direction S2, until cells that meet the predetermined requirements are positioned at a distance from the aspiration and discharge member 21 in the third direction S3. The selection and moving assembly 23 drives the aspiration and discharge member 21 to move to a predetermined position, and the driving means 251 drives the aspiration and discharge member 21 to aspirate cells that meet the predetermined requirements into the hollow conduit 211, thereby completing the aspiration of cells that meet the predetermined requirements.

[0157] Subsequently, the selection and movement assembly 23 drives the suction port 212 of the suction and discharge member 21 upward until the storage unit 3142a is positioned at a distance from the suction and discharge member 21 in the third direction S3, the first mounting and movement means 321 drives the storage means 314 in the first direction S1, and the second mounting and movement means 322 drives the storage means 314 in the second direction S2. The selection and movement assembly 23 drives the suction and discharge member 21 to a predetermined position, and the drive means 251 drives the suction and discharge member 21 to release cells that meet the predetermined requirements into the storage means 314, thereby completing the collection and culture of cells that meet the predetermined requirements.

[0158] In some embodiments, the imaging mechanism in some embodiments of the present application is shown with reference to Figures 27 and 28. The imaging mechanism 40 is used to image cells to be sorted and to acquire image information of cells to be sorted, and to select cells that meet predetermined requirements. The imaging mechanism 40 includes an imaging illumination means 41, an optical path assembly 42, a fluorescence assembly 43, and a detection assembly 44.

[0159] The imaging illumination means 41 provides a bright-field illumination light source to the sorting target member and the optical path assembly 42, irradiating the sorting target cells with transmitted light. The transmitted light reaches the detection assembly 44 via the optical path assembly 42, and the detection assembly 44 acquires image information of the sorting target cells. Referring to Figures 2 and 9, the imaging illumination means 41 is installed at a distance from the optical path assembly 42 along the third direction S3, and the sorting target member is positioned between the imaging illumination means 41 and the optical path assembly 42 in the third direction S3, so that the transmitted light emitted from the imaging illumination means 41 passes through the sorting target member and enters the optical path assembly 42, thereby achieving imaging of the sorting target cells.

[0160] Referring again to Figures 8 and 9, the imaging illumination means 41 is used to illuminate the cells to be sorted and provides a constant brightness for observation of the cells to be sorted. The imaging illumination means 41 is connected to the mounting assembly 22 along the third direction S3. Referring to Figures 2 and 9, the imaging illumination means 41 is connected to the housing member 223 along the third direction S3. The imaging illumination means 41 is closer to the suction outlet 212 of the suction discharge member 21 than the selection illumination means 24. The imaging illumination means 41 is installed spaced apart from the selection illumination means 24 and the suction discharge member 21 along the axial direction and the third direction S3.

[0161] In some embodiments, the imaging illumination means 41 includes an imaging light source 411, a light-shielding member 412, and a light-transmitting member 413. Light emitted from the imaging light source 411 reaches the target cells via the light-shielding member 412 and the light-transmitting member 413, illuminating the target cells along the outer circumference of the suction / discharge member 21. The imaging light source 411 is connected to the mounting assembly 22 along the third direction S3, enabling the light to be transmitted to the imaging mechanism 40 and satisfying the illumination requirements of the imaging mechanism 40. The position of the imaging light source 411 can be set according to the actual situation.

[0162] The imaging light source 411 is provided with a central hole through which the suction / discharge member 21 is inserted, allowing the suction / discharge member 21 to penetrate the imaging light source 411. Furthermore, referring to Figures 12 and 13, the first clamping drive member 2212 is connected to the imaging light source 411 via the central hole. The diameter of the central hole is slightly larger than the outer diameter of the second clamping drive portion 2212c of the first clamping drive member 2212. The central axis of the imaging light source 411 in the third direction S3 and the central axis of the suction / discharge member 21 in the third direction S3 coincide or are parallel, enabling illumination by the imaging light source 411.

[0163] In some embodiments, the imaging light source 411 is a ring-shaped light source that brightly and uniformly illuminates a wide field of view. Furthermore, the imaging light source 411 is an LED light source. The ring-shaped LED light source may be an integrated ring-shaped light source, or it may be a configuration in which multiple light sources are uniformly arranged at intervals in the circumferential direction. As a result, the imaging light source 411 irradiates the cell sorting target area from the radially outer side of the suction / discharge member 21. Note that the type of ring-shaped light source is not limited to an LED light source.

[0164] In some embodiments, referring to Figure 9, the imaging light source 411 and the housing member 223 are detachably connected. The detachable connection facilitates the installation and replacement of the suction / discharge member 21, while also facilitating the installation and replacement of the imaging light source 411 while preventing the suction / discharge member 21 from colliding with and damaging the imaging light source 411 during installation and replacement.

[0165] In some embodiments, when using the cell sorting device 100, the suction / discharge member 21 is first attached to the clamping means 221, then the clamping means 221 is placed in the housing member 223, and finally the imaging / illumination means 41 is connected to the housing member 223 by passing through the suction / discharge member 21 along the third direction S3. This prevents the suction / discharge member 21 from colliding with and being damaged by the imaging / illumination means 41 during installation.

[0166] In specific embodiments, the imaging light source 411 and the housing member 223 are connected by magnetic attraction. In one embodiment, a magnet is installed on the end face of the imaging light source 411, the housing member 223 is made of a metal material, and the light source cover and the housing member 223 are connected by magnetic attraction of the metal material by the magnet. In another embodiment, the housing member 223 is made of a non-metallic material and has a metal member for magnetic attraction installed, and magnetic attraction connection between the light source cover and the housing member 223 is achieved by attraction of the metal member by the magnet. It should be understood that the magnet and the metal member for magnetic attraction may be interchangeable, and the specific installation position is not limited. For example, it is also possible to achieve connection between the light source cover and the housing member 223 by installing the metal member for magnetic attraction on the light source cover and a magnet on the housing member 223. Magnetic attraction connection facilitates the removal and connection of the mounting assembly 22 and the lighting assembly 24, and simplifies the installation and replacement of the attraction / discharge member 21. Furthermore, the magnetic adsorption connection does not damage the structure of the mounting assembly 22 and the lighting assembly 24, offers excellent reliability and stability, eliminates the need for precise alignment, and allows for quick and convenient operation.

[0167] In one embodiment not shown, the imaging light source 411 is provided with a groove, and the housing member 223 is provided with a projection that engages with the groove. The connection between the groove and the projection enables a detachable connection between the mounting assembly 22 and the illumination assembly 24. It should be understood that the method of connecting the imaging light source 411 and the housing member 223 is not limited to a detachable connection, as long as a connection between the imaging light source 411 and the housing member 223 can be achieved. For example, the imaging light source 411 could be attached to the housing member 223 with adhesive.

[0168] In some embodiments, the light-transmitting member 413 and the light-shielding member 412 both limit the scattering of light from the imaging light source 411. The light-shielding member 412 is positioned between the imaging light source 411 and the light-transmitting member 413 in a third direction S3. The light-shielding member 412 has a hollow cylindrical shape overall and extends in the third direction S3, allowing the insertion of the suction / discharge member 21. The outer diameter of the light-shielding member 412 matches the outer diameter of the imaging light source 411. The light-transmitting member 413 has a transparent ring structure with a central hole. The outer diameter of the light-transmitting member 413 matches the outer diameter of the imaging light source 411, and the shape and dimensions of the central hole match the outer diameter shape and dimensions of the suction / discharge member 21, thereby allowing the insertion of the suction / discharge member 21. The light-transmitting member 413 can be made of acrylic. It should be understood that the shape, dimensions, and material of the light-shielding member 412 and the light-transmitting member 413 are not limited as long as the scattering and transmission of light can be restricted.

[0169] In some embodiments, the light-transmitting member 413, the light-shielding member 412, and the imaging light source 411 are detachably connected to facilitate inspection and replacement. Specifically, the light-transmitting member 413, the light-shielding member 412, and the imaging light source 411 all have screw holes coaxially positioned along the third direction S3, and the connection of the light-transmitting member 413, the light-shielding member 412, and the imaging light source 411 is achieved by inserting fastening members through the screw holes. Note that the imaging light source 411, the light-shielding member 412, and the light-transmitting member 413 are not limited to detachable connections, but can also be connected by adhesive.

[0170] If the imaging light source 411 is a light source for transmitted light, then the imaging light source 411 can be any light source that emits light containing wavelengths that the cells to be sorted can absorb or reflect. Various lamps such as halogen lamps, light-emitting diodes, and various lasers can be used as the imaging light source 411. The image of the cells to be sorted may be image information such as a bright-field image or a dark-field image. Furthermore, in some embodiments, by making the brightness of the imaging light source 411 adjustable, the range of application of the imaging light source 411 is expanded and the brightness of the image information can be easily adjusted.

[0171] In some embodiments, referring to Figure 28, the optical path assembly 42 is used to form the optical path of the imaging mechanism 40, and optical elements are arranged to form a transmitted light path, thereby achieving imaging of the cells to be sorted. The optical path assembly 42 includes an objective lens 421 and a first imaging lens 422. The objective lens 421 has a constant focal length, and a predetermined area of ​​the members to be sorted is positioned at a distance from the objective lens 421 along a third direction S3. The objective lens 421 forms an image of the cells to be sorted in the predetermined area. The objective lens 421 and the first imaging lens 422 are positioned at a distance from each other along the third direction S3, thereby forming the transmitted light path of the imaging mechanism 40. The transmitted light emitted from the imaging light source 411 passes sequentially through the cells to be sorted, the objective lens 421, and the first imaging lens 422, and reaches the detection assembly 44. Specifically, a 10x objective lens 421 can be used. The magnification of the objective lens 421 can be set according to actual requirements and is not limited thereto. In other embodiments, the optical path assembly 42 includes multiple objective lenses 421 to acquire image information of the cells to be sorted at different magnifications. Since the objective lens 421 and the first imaging lens 422 are installed on one side of the cells to be sorted in the third direction S3, the light generated in the cells to be sorted by the light emitted from the imaging light source 411 may be reflected light rather than transmitted light.

[0172] In some embodiments, referring to Figures 27 and 28, the optical path assembly 42 further includes an objective lens moving means 423. The objective lens moving means 423 is used to focus the objective lens 421 and to sharpen the image of the cells to be sorted by changing the focal distance between the objective lens 421 and the member to be sorted, so that the imaging mechanism 40 can acquire sharp image information of the cells to be sorted. The objective lens moving means 423 can sharpen the image information of the cells to be sorted by driving the objective lens 421 to move along a third direction S3 relative to the member to be sorted.

[0173] In some embodiments, the objective lens moving means 423 includes a first rotating member 4231 and a second rotating member (not shown), the first rotating member 4231 and the second rotating member being positioned opposite each other along a second direction S2. Both the first rotating member 4231 and the second rotating member can move the objective lens 421 along a third direction S3 to achieve focus of the objective lens 421. Of these, the second rotating member can achieve automatic movement of the objective lens 421 under the control of the control mechanism 60, while the first rotating member 4231 can achieve movement of the objective lens 421 by external force without being controlled by the control mechanism 60. By installing the first rotating member 4231 and the second rotating member, the objective lens 421 can be both automatically and manually focused, improving the applicability of the cell sorting device 100.

[0174] In some embodiments, the objective lens moving means 423 further includes an objective lens driving member 4232. The objective lens driving member 4232 is connected to the control mechanism 60 and drives a second rotating member to rotate, moving the objective lens 421 along a third direction S3, thereby adjusting the distance between the objective lens 421 and the cells to be sorted, thereby achieving focus of the objective lens 421 and enabling the acquisition of high-resolution image information of the cells to be sorted. The objective lens driving member 4232 may employ a stepping motor to precisely control the movement distance of the objective lens 421 and to achieve coarse and fine adjustments in the focus of the objective lens 421.

[0175] In some embodiments, the objective lens moving means 423 further includes a displacement sensor (not shown). The displacement sensor allows for easy control of the displacement of the objective lens 421, contributing to the autofocus of the objective lens 421. In this embodiment, a grating displacement sensor is employed. It should be understood that the type of displacement sensor is not limited as long as the positional information of the mounting assembly 31 can be obtained.

[0176] In some embodiments, the objective lens moving means 423 further includes an objective lens position limiting member (not shown). By installing the objective lens position limiting member, the movement stroke of the objective lens 421 is limited, and in cooperation with a displacement sensor, the uppermost and lowermost positions of the objective lens 421 can be obtained. This prevents the objective lens 421 from colliding with the mounting mechanism 30 and being damaged, and facilitates focusing by automatic movement of the objective lens 421.

[0177] Referring to Figures 19 to 22, the first mounting member 311 is set in a stepped structure in the third direction S3, providing the objective lens 421 with a travel distance for focusing while preventing the second mounting member 312 from colliding with the storage means 314, and preventing the objective lens 421 from colliding with the first mounting member 311 and being damaged during focusing. Furthermore, the lens surface of the objective lens 421 is positioned between the first mounting member 311 and the second mounting member 312 in the third direction S3, thereby better achieving focus of the objective lens 421 and ensuring clarity of image information of the cells to be sorted. The third mounting member 313 is provided with an observation hole 3132 to facilitate focusing of the objective lens 421 and prevent the objective lens 421 from colliding with the third mounting member 313. The second mounting member 312 is provided with a through hole for the focal point of the objective lens 421 to pass through. The size of the through-hole is set so that the second mounting member 312 does not collide with the objective lens 421 when it moves, ensuring the normal operation of the cell sorting device 100.

[0178] Referring to Figure 2, in some embodiments, the central axes of the objective lens 421 and the suction / discharge member 21 in the third direction S3 coincide or are parallel. This improves the accuracy of the suction / discharge member 21 when aspirating cells that meet predetermined requirements, and contributes to improving the sorting efficiency of the cell sorting device 100.

[0179] Referring again to Figures 27 and 28, the fluorescence assembly 43 is used to cause the cells to be sorted to emit fluorescence. Cells that meet predetermined requirements are identified from the fluorescence image information of the cells to be sorted. The fluorescence assembly 43 and the optical path assembly 42 are placed on the same side of the cells to be sorted. The fluorescence assembly 43 includes an excitation light source 431 and a filter means 432. The excitation light source 431 is used to irradiate the cells to be sorted to cause them to emit fluorescence. The excitation light source 431 can be any light source capable of causing the cells to be sorted to emit fluorescence. As the excitation light source 431, lamps such as xenon lamps and mercury lamps, various lasers such as argon lasers, and light-emitting diodes can be used.

[0180] The filter means 432 is an important component for the imaging mechanism 40 to achieve fluorescence imaging. In some embodiments, the filter means 432 is installed between the objective lens 421 and the first imaging lens 422 in the third direction S3 and includes a first beam splitter 4321. The first beam splitter 4321 can reflect light emitted from the excitation light source 431 and transmit transmitted light and fluorescence that has passed through the cells to be sorted. As a result, the detection assembly 44 acquires image information of the cells to be sorted. The first beam splitter 4321 can separate the wavelength of the imaging light source 411 and the wavelength of fluorescence generated from the cells to be sorted, and can employ, for example, a dichroic mirror. The first beam splitter 4321 is positioned between the objective lens 421 and the first imaging lens 422 in the third direction S3 and is installed at an angle to the third direction S3. The first beam splitter 4321 is installed at a distance from the excitation light source 431 along the second direction S2, so that it reflects the light emitted from the excitation light source 431 onto the cells to be sorted, causing the cells to emit fluorescence.

[0181] In some embodiments, the filtering means 432 further includes a first filter member 4322. The first filter member 4322 is positioned between the first beam splitter 4321 and the excitation light source 431 in a second direction S2. The first filter member 4322 filters the excitation light source 431, allowing light in the wavelength range that causes the target cells to emit fluorescence to reach the first beam splitter 4321.

[0182] The observation light path in the imaging mechanism 40 includes an illumination light path obtained by light emitted from the imaging light source 411 passing through the target cells, a light guide light path that directs light emitted from the excitation light source 431 to the target cells, and a fluorescence light path through which fluorescence is generated from the target cells.

[0183] In some embodiments, the fluorescence assembly 43 further includes a filter switching means 433. The filter switching means 433 is used to switch between different filter means 432. Different excitation light in different wavelength bands obtained by passing through different filter means 432 causes the target cells to emit different fluorescence. This allows the imaging mechanism 40 to meet the culture requirements of the target cells under diverse conditions, improving the applicability of the cell sorting device 100.

[0184] In some embodiments, cells to be screened are specifically screened in a culture medium to which a predetermined type of fluorescent dye is added. Different fluorescent dyes can emit different fluorescence upon excitation light. For example, cells to be screened are cultured with three types of fluorescent dyes, and after the excitation light passes through the first filter means 432, the cells to be screened emit a first fluorescence; after the excitation light passes through the second filter means 432, the cells to be screened emit a second fluorescence; and after the excitation light passes through the third filter means 432, the cells to be screened emit a third fluorescence.

[0185] In specific examples, target cells are specifically screened in a culture medium supplemented with a fluorescent dye capable of generating blue fluorescence, a fluorescent dye capable of generating green fluorescence, and a fluorescent dye capable of generating red fluorescence. Short-wavelength light generates long-wavelength light upon excitation; therefore, ultraviolet light generates blue light upon excitation, blue light generates green light upon excitation, and green light generates red light upon excitation. For target cells cultured with a fluorescent dye capable of generating blue fluorescence, an ultraviolet light filter means 432 is selected, and the target cells are made to emit blue fluorescence by excitation light. For target cells cultured with a fluorescent dye capable of generating green fluorescence, a blue light filter means 432 is selected, and the target cells are made to emit green fluorescence by excitation light. For target cells cultured with a fluorescent dye capable of generating red fluorescence, a green light filter means 432 is selected, and the target cells are made to emit red fluorescence by excitation light. By specifically screening target cells with a predetermined type of fluorescent dye, it contributes to more accurately selecting cells that meet predetermined requirements. It should be understood that different filter means 432 are selected depending on the different fluorescent dyes, and the types of fluorescent dyes and filter means 432 are not limited.

[0186] In some embodiments, the filter switching means 433 includes a switching drive member 4331. The switching drive member 4331 is connected to a filter means 432 and can be driven to move the filter means 432, thereby enabling switching between different filter means 432. The switching drive member 4331 is installed on one side of the objective lens 421 in a second direction S2. The filter means 432 connected to the switching drive member 4331 are all movable in the observation light path of the imaging mechanism 40, for example, moving between the objective lens 421 and the first imaging lens 422 in a third direction S3. Referring to Figure 2, the control mechanism 60 controls the filter switching means 433 to switch between different filter means 432, thereby exciting different fluorescent dyes and causing them to emit different fluorescence.

[0187] In some embodiments, the filter means 432 is configured with three distinct filter members: a first filter member 4322, a second filter member, and a third filter member. The filter switching means 433 switches between the first filter member 4322, the second filter member, and the third filter member. When the filter switching means 433 switches to the first filter member 4322, the light emitted from the excitation light source 431 passes through the first filter member 4322, causing the target cells to emit a first fluorescence. When the filter switching means 433 switches to the second filter member, the light emitted from the excitation light source 431 passes through the second filter member, causing the target cells to emit a second fluorescence. When the filter switching means 433 switches to the third filter member, the light emitted from the excitation light source 431 passes through the third filter member, causing the target cells to emit a third fluorescence. The number of filter members can be set according to the fluorescence emitted from the target cells.

[0188] In some embodiments, the filter switching means 433 includes a switching drive member 4331 connected to a control mechanism 60. The switching drive member 4331 is connected to an excitation light source 431, and by switching the excitation light source 431, it is possible to make the target cells emit different fluorescence. For example, by switching between three sets of excitation light sources 431 capable of exciting different fluorescence entering the observation light path, such as an ultraviolet LED light source, a blue LED light source, and a green LED light source, and emitting excitation light of different wavelengths, the target cells can emit different fluorescence. Switching between the incident and exit light paths of different light sources, switching between different filter means 432, or switching between the incident and exit light paths of different filter members by the filter switching means 433 can be done manually or automatically. The specific installation method can be set according to the actual requirements, and the method of switching the excitation light source 431 is not limited here.

[0189] In some embodiments, the filtering means 432 further includes a fourth filtering member 4323. The fourth filtering member 4323 is positioned between the first beam splitter 4321 and the first imaging lens 422 in the third direction S3. The fourth filtering member 4323 filters fluorescence emitted from the cells to be sorted, so that the fluorescence image information of the cells to be sorted that reaches the detection assembly 44 is not affected by other light.

[0190] The filter means 432 plays an important role in fluorescence imaging by the imaging mechanism 40. Specifically, when light emitted from the excitation light source 431 is irradiated onto the first filter member 4322, the first filter member 4322 selects the excitation light source 431 in a certain wavelength band to excite the sample and blocks light of other wavelengths. The light that has passed through the first filter member 4322 passes through the first beam splitter 4321. The filtered excitation light is reflected by the first beam splitter 4321, focused by the objective lens 421, and irradiated onto the target cells to emit the corresponding fluorescence. The fluorescence is collected by the objective lens 421, passes through the first beam splitter 4321, and reaches the fourth filter member 4323. When the first beam splitter 4321 is tilted at 45 degrees with respect to the excitation light source 431, the filtered excitation light is incident perpendicularly on the objective lens 421, in which case the objective lens 421 functions as a focusing lens. In one embodiment, the first filter member 4322, the fourth filter member 4323, and the first beam splitter 4321 are installed together to constitute the filter means 432.

[0191] Referring to Figure 28, the detection assembly 44 is connected to a display mechanism 50 used for observing and processing images. The detection assembly 44 detects image information formed by transmitted light and fluorescence of cells to be selected ejected from the optical path assembly 42, processes and acquires the obtained image information, and transmits the acquired image information to the display mechanism 50. In one embodiment, the detection assembly 44 includes a CCD sensor 441. In another embodiment, the detection assembly 44 includes a CMOS sensor 441. Both the CMOS sensor 441 and the CCD sensor 441 can acquire bright-field image information and fluorescence image information of the cells to be selected, facilitating subsequent processing and screening. Furthermore, the detection assembly 44 further includes cooling means (not shown). For example, cooling the CMOS sensor 441 or CCD sensor 441 with a cooling means such as liquid nitrogen or a Peltier element improves the signal-to-noise ratio and reduces noise in the image information.

[0192] In some embodiments, the detection assembly 44 includes a bright-field image information detector 442 and a fluorescence image information detector 443. The bright-field image information detector 442 is used to detect image information of transmitted and reflected light from the imaging light source 411 that has passed through the cells to be sorted. The fluorescence image information detector 443 is used to detect image information of fluorescence emitted from the cells to be sorted. Both the bright-field image information detector 442 and the fluorescence image information detector 443 are connected to a CMOS sensor 441 or a CCD sensor 441.

[0193] In some embodiments, the optical path assembly 42 is equipped with a second beam splitter 424 and a second imaging lens 425. The second beam splitter 424 is spaced apart from the first beam splitter 4321 along the third direction S3 and is positioned between the first beam splitter 4321 and the first imaging lens 422. Transmitted light from microcells that has passed through the first beam splitter 4321 passes through the second beam splitter 424 and the first imaging lens 422 and is emitted to the bright-field image information detector 442. Furthermore, a filter (not shown) is placed between the first imaging lens 422 and the bright-field image information detector 442 so that the transmitted light enters the bright-field image information detection unit 442 without interference with the excitation light source 431 and fluorescence.

[0194] The second imaging lens 425 is positioned between the second beam splitter 424 and the fluorescence image information detector 443. Fluorescence from the target cells that have passed through the first beam splitter 4321 is reflected by the second beam splitter 424, passes through the second imaging lens 425, and is then emitted to the fluorescence image information detector 443. Furthermore, a filter (not shown) is positioned between the imaging lens and the fluorescence image information detector 443 so that the fluorescence enters the fluorescence image information detector 443 without interference from the excitation light source 431 and the transmitted light.

[0195] The first imaging lens 422 for the bright-field image information detector 442 and the second imaging lens 425 for the fluorescence image information detector 443 may be the same lens or different lenses. For example, it is possible to decrease the magnification of the first imaging lens 422 for the bright-field image information detector 442 and increase the magnification of the second imaging lens 425 for the fluorescence image information detector 443. In this case, the field of view of the transmitted light image acquired by the bright-field image information detector 442 can be expanded, and an even more magnified fluorescence image of the target cells for selection can be acquired by the fluorescence image information detector 443.

[0196] In some embodiments, referring to Figures 2 and 27, the imaging mechanism 40 further includes a protective housing 45 to protect the internal assembly of the imaging mechanism 40, prevent the optical path of the imaging mechanism 40 from being affected by external light, and ensure accurate and clear image information of cells to be sorted. The protective housing 45 is connected to the base plate 1211 by fastening members. The base plate 1211 is further provided with fixing protrusions for fixing the protective housing 45, and the positional restriction of the protective housing 45 by the multiple fixing protrusions ensures the stable positioning of the imaging mechanism 40.

[0197] Before using the imaging mechanism 40, the imaging illumination means 41, the cells to be sorted, and the objective lens 421 are set up along the third direction S3. When in use, bright-field image information of the cells to be sorted is acquired. The specific operation is as follows: The imaging illumination means 41 is turned on, and light emitted from the imaging light source 411 passes through the cells to be sorted, the objective lens 421, the filter means 432, and the first imaging lens 422 and enters the bright-field image information detector 442, which transmits the acquired image information of the cells to be sorted to the display mechanism 50. Based on the image information displayed on the display mechanism 50, the objective lens moving means 423 is controlled to move the objective lens 421 along the third direction S3, thereby sharpening the image information of the cells to be sorted. The image is recorded by the CMOS sensor 441 or a CMOS sensor and used for subsequent processing.

[0198] Fluorescence image information of cells to be screened is acquired. The specific operation is as follows: The imaging light source 411 is turned off, and the excitation light source 431 is turned on. Light emitted from the excitation light source 431 passes through the filter means 432 and the objective lens 421 to reach the cells to be screened, causing the cells to be screened specifically with the fluorescent dye to emit fluorescence. The fluorescence passes through the objective lens 421, the filter means 432 and the second imaging lens 425 and enters the fluorescence image information detector 443, which transmits the fluorescence image information of the cells to be screened to the display mechanism 50. Based on the image information acquired by the display mechanism 50, the objective lens moving means 423 is controlled to move the position of the objective lens 421, thereby sharpening the image information. The image information is then recorded by the CCD sensor 441 or CMOS sensor. Different filter means 432 are switched by the filter switching means 433, and the above operation is repeated to transmit different fluorescence image information of the cells to be screened to the display mechanism 50. By analyzing the bright-field and fluorescence image information of the selected cells, cells that meet the specified requirements are identified.

[0199] The switching between the imaging light source 411 and the excitation light source 431 may be achieved by the control mechanism 60 or by manual switching.

[0200] The components to be sorted can be automatically moved under the control of the control mechanism 60. Different filter means 432 are installed in the fluorescence assembly 43, which are automatically switched by the filter switching means 433, and the objective lens moving means 423 automatically adjusts the focus, enabling automatic scanning of the imaging mechanism 40. This enables automatic scanning and imaging of a large number of cells to be sorted, significantly improving the work efficiency of the cell sorting device 100 and expanding its range of application. In addition, more accurate cell sorting is achieved through comparative analysis of various fluorescence image information and bright-field image information before and after selection.

[0201] Referring to Figure 29, in some embodiments, the control mechanism 60 controls the cell sorting device 100 to achieve automatic cell sorting, improve cell sorting efficiency, and achieve high-throughput sorting. The control mechanism 60 can control the selection drive member 231 to control the movement and stopping of the suction / discharge member 21 in a third direction S3. The control mechanism 60 can control the on and off of the selection light source 241 to enable observation and alignment of the suction / discharge member 21. The control mechanism 60 can control the drive means 251 to enable cleaning of the suction / discharge member 21 and to control the suction or discharge of cells that meet predetermined requirements by the suction / discharge member 21. The control mechanism 60 can control the first mounting drive member 3211 and the second mounting drive member 3221 to control the movement and stopping of the mounting assembly 31. The control mechanism 60 can control the on and off of the imaging light source 411 to provide a transmitted light source to the cells to be sorted and to acquire bright-field image information of the cells to be sorted. The control mechanism 60 can control the on / off state of the excitation light source 431 to provide it to the cells to be sorted and to acquire fluorescence image information of the cells to be sorted. The control mechanism 60 can control the objective lens driving member 4232 to focus the objective lens 421 and acquire clear image information of the cells to be sorted. The control mechanism 60 can control the switching driving member 4331 to excite the cells to be sorted and cause them to emit different fluorescence.

[0202] In some embodiments, the control mechanism 60 is installed in the main body 10 and includes power supply modules, switch modules, conversion modules, and voltage modules, which are not shown. It should be understood that all components used to control each functional member of the cell sorting device 100 belong to the control mechanism 60, and that the control mechanism 60 can select appropriate control elements according to the assembly settings of the cell sorting device 100.

[0203] In some embodiments, the cell sorting apparatus 100 further includes an illumination assembly. The illumination assembly is used to illuminate the imaging mechanism 40 to enable observation and alignment of the suction / discharge member 21 in the display mechanism 50, while also illuminating the hollow conduit 211 of the suction / discharge member 21. The illumination assembly includes one or both of the sorting illumination means 24 and the imaging illumination means 41 described above.

[0204] In some embodiments, the operation method of the cell sorting device 100 includes the following steps.

[0205] Attach the suction discharge member 21 to the mounting assembly 22. Specifically, first, move the first housing 111 to open the cell sorting device 100. Loosen the first fixing member 2221 and the second fixing member 2222, remove the clamping means 221, and loosen the first clamping drive member 2212. At this time, be careful not to completely detach the first clamping drive member 2212 from the clamping body 2211. Place the end of the suction discharge member 21 that is positioned opposite the suction discharge port 212 on the clamping means 221, and tighten the first clamping drive member 2212 to the clamping marker 2218. Note that be careful not to overtighten, as this may damage the suction discharge member 21. Return the clamping means 221 with the suction discharge member 21 fixed to it to the housing member 223. Fix the clamping means 221 by tightening the first fixing member 2221 and the second fixing member 2222. This completes the attachment of the suction / discharge member 21 to the mounting assembly 22.

[0206] The position of the suction / discharge member 21 is determined. Specifically, under the control of the control mechanism 60, the selection light source 241 is turned on, and the selection / movement assembly 23 moves the suction / discharge member 21 in the third direction S3 until the projected image information of the suction / discharge member is clearly displayed on the display mechanism 50. The projection marker is positioned at a predetermined location in the projection mapping of the suction / discharge member by making fine adjustments to the suction / discharge member 21 using the first fixing member 2221 and the second fixing member 2222. After that, the sorting target member on which no sorting target cells are placed is placed in the fixing groove of the first mounting member 311. The selection / movement assembly 23 moves the suction / discharge member 21 along the third direction S3 until the image information of the suction / discharge port 212 is clearly displayed on the display mechanism 50. The position information of the suction / discharge member 21 at this point is recorded, completing the position determination of the suction / discharge member 21.

[0207] The first cleaning of the suction discharge member 21 is performed. Specifically, an appropriate amount of the first cleaning liquid 2541 is added to the first storage means 2551, the first storage means 2551 with the first cleaning liquid 2541 added is placed on the first mounting member 311, and the first storage means 2551 is positioned at a distance from the suction discharge member 21 along the third direction S3. Under the control of the control mechanism 60, the selection drive member 231 is driven to move the suction discharge port 212 of the suction discharge member 21 into the first cleaning liquid 2541. The drive means 251 is driven to draw the first cleaning liquid 2541 into the hollow conduit 211 of the suction discharge member 21 to perform cleaning, and then to discharge the first cleaning liquid 2541 into the first storage means 2551. This is repeated multiple times to complete the first predetermined number of primary cleanings. Subsequently, an appropriate amount of the second cleaning solution 2542 is added to the second storage means 2552, and the second storage means 2552 with the added cleaning solution 2542 is placed on the first mounting member 311, and the second storage means 2552 is positioned at a distance from the suction discharge member 21 along the third direction S3. The selection drive member 231 drives the suction discharge member 21 to move to the second cleaning solution 2542. The drive means 251 drives the suction discharge member 21 to draw the second cleaning solution 2542 into the hollow conduit 211 of the suction discharge member 21 to perform cleaning, and then drives the suction discharge member 21 to discharge the second cleaning solution 2542 into the second storage means 2552, and this is repeated multiple times to complete the second predetermined number of secondary cleanings.

[0208] The sorting target member and storage means 314 are placed. The sorting target member on which the sorting target cells are placed is placed on the mounting portion 3111 of the first mounting member 311, and by pulling the elastic member 331 located on one side of the mounting portion 3111 toward the other side of the mounting portion 3111, the elastic member 331 eccentrically presses the sorting target member, thereby achieving the placement and fixing of the sorting target member on the first mounting member 311. The storage means 314 containing an appropriate amount of culture medium is placed on the mounting groove 3112 of the first mounting member 311. The sorting target cells are specifically screened with a culture medium containing a fluorescent dye.

[0209] Cells that meet predetermined requirements are identified. Under the control of the control mechanism 60, the imaging light source 411 is turned on, and the first placement and moving means 321 and the second placement and moving means 322 are driven to position the cells to be observed within the sorting target member along the second light source and objective lens 421 and the third direction S3. The objective lens moving means 423 is driven to move the objective lens 421 until the image information of the cells to be observed is clearly displayed on the display mechanism 50. Bright-field image information of the cells to be sorted is recorded. The imaging light source 411 is turned off, and the excitation light source 431 is turned on. Light emitted from the excitation light source 431 passes through the first filter means 432, causing the cells to be sorted to emit first fluorescence. First fluorescence image information of the cells to be sorted is recorded. By controlling the filter switching means 433 to switch to the second filter means 432, the cells to be selected emit a second fluorescence, and the second fluorescence image information of the cells to be selected is recorded. By controlling the filter switching means 433 to switch to the third filter means 432, the cells to be selected emit a third fluorescence, and the third fluorescence image information of the cells to be selected is recorded. Then, the excitation light source 431 is turned off and the imaging light source 411 is turned on. The first mounting and moving means 321 and the second mounting and moving means 322 are driven to move the members to be selected to a predetermined area, and the cells to be selected to be observed are exchanged. By repeating the above process, bright-field image information and fluorescence image information of the cells to be selected in different areas are recorded. The acquired bright-field image information and fluorescence image information of the cells to be selected are processed and analyzed to identify cells that meet predetermined requirements.

[0210] Cells that meet predetermined requirements are aspirated and collected. Under the control of the control mechanism 60, the selection and movement assembly 23 moves the aspiration and discharge member 21 along the third direction S3 until it reaches a position where the aspiration and discharge member 21 can aspirate cells. This position is designated as the predetermined position, and preliminary aspiration is completed. The second placement and movement means 322 moves the second placement member 312 in the second direction S2, and the first placement and movement means 321 moves the first placement member 311 in the first direction S1, until cells that meet the predetermined requirements are placed at a distance from the aspiration and discharge member 21 along the third direction S3. The selection and movement assembly 23 moves the aspiration and discharge member 21 to the predetermined position in the third direction S3. The drive means 251 generates an appropriate negative pressure in the hollow conduit 211 of the aspiration and discharge member 21, and aspirates the cells into the hollow conduit 211 of the aspiration and discharge member 21. The selection and movement assembly 23 moves the suction and discharge member 21 away from the mounting assembly 31. The second mounting and movement means 322 moves the second mounting member 312 in the second direction S2 and the first mounting and movement means 321 moves the first mounting member 311 in the first direction S1 until the storage section 3142a for collecting and culturing cells that meet predetermined requirements is positioned at a distance from the suction and discharge member 21 along the third direction S3. The selection and movement assembly 23 moves the suction and discharge member 21 in the third direction S3 such that there is a predetermined distance between the suction and discharge port 212 and the storage section 3142a. The predetermined distance is set so that the suction and discharge member 21 can discharge cells that meet predetermined requirements into the storage section 3142a. The first driving means drives the suction and discharge member 21 to discharge cells that meet predetermined requirements, which have been aspirated into the hollow conduit 211, into the storage section 3142a. The selection and movement assembly 23 moves the suction and release member 21 away from the mounting assembly 31. By repeating the above process, different cells that meet the predetermined requirements are suctioned and released and collected in different storage units 3142a until all cells that meet the predetermined requirements within the member to be selected have been suctioned and released. The member to be selected is collected and stored, the storage means 314 is transferred to the culture vessel, and the culture is continued.

[0211] A second cleaning of the suction discharge member 21 is performed. Specifically, an appropriate amount of the second cleaning solution 2542 is added to the second storage means 2552, the second storage means 2552 with the second cleaning solution 2542 added is placed on the first mounting member 311, and the second storage means 2552 is positioned at a distance from the suction discharge member 21 along the third direction S3. The selection drive member 231 drives the suction discharge member 21 to move to the second cleaning solution 2542. The drive means 251 drives the suction discharge member 21 to draw the second cleaning solution 2542 into the hollow conduit 211 of the suction discharge member 21 and perform cleaning, and then to discharge the second cleaning solution 2542 into the second storage means 2552, and this is repeated multiple times to complete a predetermined number of primary cleanings. Subsequently, an appropriate amount of the first cleaning solution 2541 is added to the first storage means 2551, and the first storage means 2551 with the first cleaning solution 2541 added is placed on the first mounting member 311, and the first storage means 2551 is positioned at a distance from the suction discharge member 21 along the third direction S3. Under the control of the control mechanism 60, the selection drive member 231 drives the suction discharge port 212 of the suction discharge member 21 to move to the first cleaning solution 2541. The drive means 251 drives the suction discharge member 21 to perform cleaning by drawing the first cleaning solution 2541 into the hollow conduit 211 of the suction discharge member 21, and then to discharge the first cleaning solution 2541 into the first storage means 2551, and this is repeated multiple times to complete a predetermined number of secondary cleanings.

[0212] Furthermore, the operating method of the cell sorting device 100 includes the following:

[0213] Bright-field image information of the aspirated cells to be sorted is acquired. Specifically, before the second washing of the aspiration / discharge member 21, after aspirating and collecting cells that meet predetermined requirements, the imaging light source 411 is turned on under the control of the control mechanism 60. The first mounting / moving means 321 and the second mounting / moving means 322 drive the cells to be observed within the sorting member to be positioned along the second light source and objective lens 421 and the third direction S3. The objective lens moving means 423 drives the objective lens 421 to move until the image information of the cells to be observed is clearly displayed on the display mechanism 50. Bright-field image information of the cells to be sorted is recorded. The first mounting / moving means 321 and the second mounting / moving means 322 drive the sorting member to move to a predetermined area and exchange the cells to be observed. The above process is repeated until the bright-field image information of the cells to be sorted in different areas is recorded. The bright-field image information of the target cells before and after aspiration is compared to obtain the status of cells acquired that meet the predetermined requirements.

[0214] The cell sorting device 100 selectively aspirates cells that meet predetermined requirements within the sorting target material and releases them into the corresponding storage section 3142a of the storage means 314. Since this process is completed automatically under the control of the control mechanism 60, cell sorting efficiency is improved, damage to the aspirator / discharge member 21 and cells that meet the predetermined requirements due to manual operation is avoided, the biological activity of the cells that meet the predetermined requirements is guaranteed, and high-throughput cell sorting is achieved.

[0215] The BioCulture Chip 200 fully utilizes microscopy or nanotechnology to achieve precise control of cell capture, fixation, and culture on the chip, and achieves research objectives such as high throughput, multi-parameter analysis of cell samples, detection of in situ signals, and physicochemical analysis of cellular components through miniaturized chemical analysis methods.

[0216] The following provides a detailed description of the Bio Culture Chip 200.

[0217] Figures 30 to 32 show schematic diagrams of bioculture chips in several embodiments of the present invention.

[0218] In some embodiments, referring to Figures 30 to 32, the bioculture chip 200 includes a substrate 201 and a containment chamber 202. The substrate 201 is formed of a substrate material, and the containment chamber 202 is formed on the surface of the substrate 201 and opens to the surface of the substrate 201. The containment chamber 202 defines a bioculture space for culturing cellular biological material. The structure and dimensions of the containment chamber 202 are not particularly limited, as long as it can provide space for culturing or growing a single cell. The containment chamber 202 may have a structure such as a rectangular parallelepiped, a cube, or a cylinder.

[0219] In some embodiments, a microporous soft substrate with excellent biocompatibility, compatibility with cell culture media, and mechanical tension is selected as the substrate material, enabling more efficient capture and culture of cells (including single cells and multicellular groups of controllable numbers). The soft substrate exhibits excellent affinity and fusion with different extracellular matrix environments, providing a uniform and fused three-dimensional culture space for cells. This creates a microenvironment favorable to the survival, proliferation, migration, protein expression, secretion, and stem cell differentiation of two-dimensionally arranged cells, and is suitable for detecting the biological functions of arranged cells. The specific types of substrate materials that can be used are not particularly limited, and rigid substrate materials such as glass, silicon wafers, and plastics are also included within the scope of protection of this application.

[0220] In some embodiments, referring to Figure 32, the opening diameter of the containment chamber 202 is smaller than the bottom diameter of the containment chamber 202. This facilitates the capture and culture of single cells and prevents cell escape during the culture process. Specifically, the opening diameter of the containment chamber 202 is 80% or less of the bottom diameter of the containment chamber 202, preferably 50%. The term "diameter" is defined as the diameter of the circle with the maximum area that the opening or bottom can cover. The term "depth" of the containment chamber 202 refers to the shortest distance between the opening cross section and the bottom cross section of the containment chamber 202. In one embodiment, the opening diameter of the containment chamber 202 is 8 to 25 μm, and the depth is 15 to 35 μm. Note that different opening diameters can be set for different cells. For example, if the biomaterial is B cells, the opening diameter is 8 to 12 μm, and if the biomaterial is hybridoma cells or tumor cells, the opening diameter is 15 to 25 μm.

[0221] In some embodiments, the bioculture chip 200 may include a plurality of discretely distributed containment chambers 202. The containment chambers 202 form a predetermined pattern, with the distance between two adjacent containment chambers 202 being 10 to 100 μm. This allows for batch processing of multiple single-cell cultures. When the distance between containment chambers 202 is in the range of 10 to 100 μm, the cells cultured in the containment chambers 202 do not interfere with each other, thereby improving the uniformity of batch processing, improving the accuracy and efficiency of detecting the biological function of cells, and facilitating high-throughput collection and analysis of cell signal data. The containment chambers 202 on the bioculture chip 200 may form an array. For example, an array consisting of tens of thousands, hundreds of thousands, or even more containment chambers 202 may be formed on a single chip 200.

[0222] In some embodiments, the bioculture chip 200 may be further equipped with positioning markers formed on the substrate 201. This allows for the positioning of multiple containment chambers 202 by these positioning markers. The form of the positioning markers is not particularly limited, and according to embodiments of the present invention, fluorescent positioning markers can be installed to enable automatic and accurate positioning, or coordinate lines can be installed to position each containment chamber 202. When cells that meet a predetermined requirement are observed, each containment chamber 202 in which the cells that meet the predetermined requirement are placed can be quickly positioned and recorded. This makes it easier to subsequently separate the cells that meet the predetermined requirement or other components within the containment chamber 202.

[0223] In some examples, the method of using the bio-culture chip 200 is as follows:

[0224] The chip 200 is placed on the chip housing member 71. Since the bioculture chip 200 needs to be stored in a low-temperature environment, for example, 0-5°C, it is necessary to leave the bioculture chip 200 at room temperature for a certain period of time before use to allow its temperature to return to room temperature. Because the bioculture chip 200 is thinly formed and its material is soft and brittle, it is usually placed inside the chip housing member 71. After the chip housing member 71 on which the bioculture chip 200 is placed is disinfected and sterilized on the outside, it is transferred to a sterile environment, the chip storage solution is collected by pipette, and it is washed multiple times with sterile PBS buffer at a concentration of 0.01M. Care should be taken to avoid contact with the bioculture chip 200 during washing.

[0225] Cells are added. The cells to be selected, which have been prepared in advance, are added to the chip housing member 71 containing the bio-culture chip 200. To selectively ensure the efficiency of the optimal single-cell array, the ratio of cell number to chip throughput is set to a range of 5:1 to 10:1, and the volume of the cell suspension is set to 1.5 ml. For example, if the chip throughput is 100,000, the array is formed with a suspension containing 500,000 to 1,000,000 cells. The cells are left to stand in the cell culture incubator for a predetermined time, for example, 15 minutes, to allow the cells to be selected to form an array on the bio-culture chip 200, and the housing chamber 202 of the bio-culture chip 200 is used as the array.

[0226] Perform the first separation. After collecting the cell suspension after array formation using a pipette, wash it multiple times with 0.01 M PBS buffer to remove any remaining cells. Alternatively, the tip housing member 71 may be washed with the tip tilted appropriately. It is also necessary to carefully separate any cells that have not formed into the array. Observe the array state of the washed cell array-formed tip 200 under a microscope.

[0227] Prepare the screening medium. Prepare a complete cell medium in advance, add the specified types of fluorescent dyes to the complete medium at the required dilution ratio, and shake slowly to mix thoroughly. If necessary, add the labeled substance, such as an antigen that has been previously labeled with a fluorescent dye, to the thoroughly mixed complete medium at the specified dilution ratio, shake slowly to mix thoroughly, and obtain the screening medium.

[0228] The screening medium is added. After adding the pre-prepared screening medium to the chip 200 after cell array formation, the chip 200 is placed in a cell culture incubator and cultured. The substances in the screening medium specifically bind to the substances secreted by the target cells after array formation. The culture time can be set according to different projects; for example, 3 to 8 hours for cell line development projects and 8 hours or more for antibody discovery projects. In antibody discovery projects, an antigen pre-labeled with a fluorescent dye is added to the screening medium to specifically bind to the antibody secreted by the cells. The antigen pre-labeled with a fluorescent dye functions as a marker. In cell line development projects, a secondary antibody pre-labeled with a fluorescent dye is added to the screening medium to specifically bind to the antibody secreted by the cells. The secondary antibody pre-labeled with a fluorescent dye functions as a marker.

[0229] A second isolation is performed. After the culture is complete, the screening medium is collected and the chip is washed 3 to 5 times with 0.01 M PBS buffer until almost all suspension cells are removed. The cells to be selected after fluorescent staining are then obtained in Bio Culture Chip 200.

[0230] Figure 33 shows a schematic diagram of a tip fixing assembly in some embodiments of the present application, and Figure 34 shows a schematic diagram of a pressurizing member in some embodiments of the present application.

[0231] In some embodiments, referring to Figures 19 and 33, the bioculture chip 200 is thinly formed and made of a soft and brittle material, making it difficult to fix directly to the cell sorting device 100. Therefore, the cell sorting device 100 further includes a chip fixing assembly 70. The chip fixing assembly 70 is mountable on a mounting mechanism 30 and includes a chip housing member 71 for housing the bioculture chip 200. First, the bioculture chip 200 is fixed to the chip housing member 71, and then the chip housing member 71 is placed on the mounting assembly 31 of the cell sorting device 100.

[0232] In some embodiments, referring to Figure 33, the chip fixing assembly 70 further includes a pressurizing member 72 supported by the chip housing member 71 in order to fix the bioculture chip 200 to the chip housing member 71. The pressurizing member 72 can stably place the bioculture chip 200 on the chip housing member 71 by applying pressure to the bioculture chip 200 along a third direction S3. The pressurizing member 72 is cylindrical in shape with both ends open, and stably places the bioculture chip 200 on the chip housing member 71 by applying pressure to the corners of the bioculture chip 200. Applying pressure to the corners of the bioculture chip 200 can increase the number of effective containment chambers 202 within the bioculture chip 200, thereby increasing the number of cells to be sorted. It should be understood that the position of pressure applied by the pressurizing member 72 is not limited as long as the fixation of the bioculture chip 200 can be achieved, for example, by applying pressure to the edges of the bioculture chip 200. Furthermore, in order to more securely fix the bio-culture chip 200 to the chip housing member 71, the corners of the bio-culture chip 200 are brought into contact with the inner wall of the chip housing member 71.

[0233] Referring to Figure 34, the pressurizing member 72 includes a pressurizing section 722 and a removal section 721 in order to facilitate the placement and removal of the pressurizing member 72 and the bio-culture chip 200. Referring to Figure 33, the pressurizing section 722 is housed in the chip housing member 71 and is used to pressurize the bio-culture chip 200. The shape and dimensions of the pressurizing section 722 conform to the shape and dimensions of the chip housing member 71. The removal section 721 is used for placing and removing the pressurizing member 72. The end of the pressurizing section 722 away from the bio-culture chip 200 extends circumferentially to form the removal section 721. The removal section 721 protrudes beyond the chip housing member 71 along the circumferential direction, i.e., at least a portion of the dimensions of the removal section 721 is larger than the dimensions of the chip housing member 71, thereby facilitating the removal of the pressurizing member 72.

[0234] In some embodiments, the pressurizing member 72 can pressurize the bioculture chip 200 by itself. Specifically, the pressurizing member 72 is made of a metal material that is heavier than the bioculture chip 200, for example, copper or aluminum, and pressurizes the bioculture chip 200 by gravity. The mass of the pressurizing member 72 can be set according to actual requirements and is not limited thereto. The material of the pressurizing member 72 is set so as not to contaminate the biochip, and disinfection and sterilization treatment must be performed before and after use of the pressurizing member 72.

[0235] In some embodiments, the pressurizing member 72 may pressurize the bio-culture chip 200 by transmitting external pressure. Specifically, the pressurizing member 72 is made of a lightweight material. The pressurizing member 72 is provided with an engaging portion, and the chip housing member 71 is provided with an engaged portion. The connection between the engaging portion and the engaged portion connects the pressurizing member 72 to the chip housing member 71 and generates pressure on the bio-culture chip 200 in the pressurizing member 72. This fixes the bio-culture chip 200 to the chip housing member 71. It should be understood that the method of pressurizing the pressurizing member 72 is not limited as long as the fixation of the bio-culture chip 200 to the chip housing member 71 can be achieved.

[0236] In some embodiments, to ensure the proper use of the bioculture chip 200 in the cell sorting device 100, the cell sorting device 100 further includes a fixation mechanism. The fixation mechanism includes a chip fixation assembly 70 and a placement position limiting assembly 33. The chip fixation assembly 70 is used to fix the bioculture chip 200 to the chip housing member 71, and the placement position limiting assembly 33 is used to fix the chip housing member 71 to the placement mechanism 30. The use of the bioculture chip 200 improves the sorting efficiency and accuracy of the cell sorting device 100.

[0237] In some embodiments, when a bioculture chip 200 in which cells have been specifically screened is used as the target material for selection, first, the bioculture chip 200 is fixed, the metal pressure member 72 is disinfected and sterilized, the metal pressure member 72 is placed on the chip housing member 71, and the bioculture chip 200 in which the pre-prepared target cells have been cultured is fixed to the chip housing member 71. Then, the chip housing member 71 is fixed and placed on the mounting portion 3111 of the first mounting member 311. The mounting portion 3111 has through holes and position-restricting walls formed around the through holes. The elastic member 331 is installed on the outer surface of the chip housing member 71, and by pulling the elastic member 331 on one side of the mounting portion 3111 to the other side of the mounting portion 3111, the elastic member 331 eccentrically presses the outer surface of the housing member 71, and the outer surface of the chip housing member 71 is restricted in position by the position-restricting wall that is eccentrically pressed. As a result, the chip housing member 71 is fixed to the first mounting member 311, and the bio-culture chip 200 is fixed to the cell sorting device 100. Thereafter, the operation method of the cell sorting device 100 described above is repeated to achieve cell sorting.

[0238] In one embodiment, the mounting position limiting assembly further includes a position limiting member 333, such as a silicone rubber cover. The chip housing member 71 is placed on the position limiting member 333, and the position limiting member 333 is placed on the mounting portion 3111, and the elastic member 331 eccentrically presses the outer surface of the position limiting member 333 to fix the chip housing member 71 to the first mounting portion 3111. It should be understood that the position limiting member 333 may be placed on the mounting portion 3111 first, and then the chip housing member 71 may be placed on the position limiting member 333.

[0239] During aspiration, the placement and movement assembly 32 drives the containment chamber 202 of the bioculture chip 200 on which cells meeting a predetermined requirement are placed until the containment chamber 202 is positioned at a distance from the aspiration discharge member 21 along the third direction S3. The selection and movement assembly 23 drives the aspiration discharge member 21 along the third direction S3 until there is a predetermined distance between the aspiration discharge port 212 and the containment chamber 202. This predetermined distance is set so that the drive means 251 drives the aspiration discharge member 21 to draw cells meeting a predetermined requirement into the hollow conduit 211 of the aspiration discharge member 21. Selectively, cells meeting a predetermined requirement are single cells contained in the containment chamber 202 of the bioculture chip 200.

[0240] The technical features of the embodiments described above can be combined in any way. For the sake of brevity, not all possible combinations of the technical features of the embodiments described above have been explained. Any combination of these technical features is included within the scope of this specification, provided that there is no contradiction.

[0241] The embodiments described above specifically and in detail represent some embodiments of the present application, but this should not be interpreted as limiting the scope of the claims. A person skilled in the art may make some modifications and improvements without departing from the spirit of the present application, and all such modifications shall also fall within the scope of protection of the present application. Accordingly, the scope of protection of the present invention shall be subject to the attached claims.

Claims

1. The main unit and A mounting mechanism configured to be attached to the main body, comprising a first mounting member on which a sorting target member containing sorting target cells is placed, and which moves relative to the main body to move the sorting target member to a predetermined area relative to the main body, An imaging mechanism attached to the main body and for acquiring image information of cells to be sorted that are placed on the aforementioned placement mechanism, the imaging mechanism including an objective lens configured to form an image on the cells to be sorted in a predetermined region, A display mechanism for displaying image information of the cells to be sorted, acquired by the imaging mechanism, A selection mechanism configured to be attached to the main body for aspirating cells that meet predetermined requirements within the sorting target member, the selection mechanism including an aspiration / discharge member configured to move relative to the main body and aspirate and release cells that meet the predetermined requirements, A cell sorting device comprising a control mechanism configured to be attached to the main body, for controlling the movement of the mounting mechanism relative to the main body, the movement of the suction / discharge member relative to the main body, and the suction and discharge of cells that meet the predetermined requirements by the suction / discharge member.

2. The cell sorting apparatus according to claim 1, wherein the placement mechanism includes a placement assembly on which the members to be sorted are placed and a placement movement assembly, the placement assembly being positioned on the placement movement assembly, and the placement movement assembly being driven to move the placement assembly so that the members to be sorted are positioned between the suction discharge member and the objective lens.

3. The cell sorting apparatus according to claim 2, wherein the mounting assembly is placed in the mounting and moving assembly and includes a storage means for collecting cells that meet the predetermined requirements, and the mounting and moving assembly is driven to move the storage means so that the storage means is positioned between the suction and discharge member and the objective lens.

4. The cell sorting apparatus according to claim 2, further comprising a fixing mechanism for fixing a bioculture chip, which is configured as a sorting target member for containing the sorting target cells, to a mounting mechanism.

5. The cell sorting apparatus according to claim 4, wherein the fixing mechanism includes a chip fixing assembly and a placement position limiting assembly, the chip fixing assembly includes a chip receiving member for accommodating the bioculture chip and is placeable on the placement assembly, and the placement position limiting assembly is installed on the placement assembly and can fix the chip receiving member to the placement assembly.

6. The cell sorting apparatus according to claim 1, wherein the imaging mechanism includes an imaging illumination means, an optical path assembly, and a detection assembly, the imaging illumination means being connected to the selection mechanism and used to illuminate the sorting target member and the optical path assembly, the sorting target member being positioned between the imaging illumination means and the optical path assembly, the optical path assembly being used to image the sorting target cells and to transmit image information of the sorting target cells to the detection assembly, and the detection assembly being connected to the display mechanism and used to acquire image information of the sorting target cells acquired by the optical path assembly.

7. The cell sorting apparatus according to claim 6, wherein the imaging mechanism further includes a fluorescence assembly, the fluorescence assembly being located on the same side as the optical path assembly and the cells to be sorted, and including an excitation light source and at least one filter means for filtering excitation light emitted from the excitation light source, the light emitted from the excitation light source and passing through the filter means causing the cells to be sorted to emit fluorescence.

8. The cell sorting apparatus according to claim 1, wherein the selection mechanism further includes a mounting assembly, a selection movement assembly, and a retrieval assembly attached to the main body, the mounting assembly to which the suction discharge member is attached, the selection movement assembly is driven to move the suction discharge member so that the suction discharge member approaches or moves away from the mounting mechanism, and the retrieval assembly is connected to the suction discharge member so that the suction discharge member can aspirate cells that meet the predetermined requirements.

9. The cell sorting apparatus according to claim 8, wherein the selection mechanism is configured to be attached to the mounting assembly and further includes a clamping means for clamping and releasing the suction / discharge member, the clamping means includes at least one clamping member that moves between a clamping position and a release position, in which case the clamping member clamps at least a portion of the suction / discharge member, and in which case the clamping member releases the suction / discharge member.

10. The cell sorting apparatus according to claim 9, wherein the clamping means further includes a first clamping drive member and a clamping body configured to which the first clamping drive member and the clamping member are attached, and the first clamping drive member is configured to drive the clamping member to move between a clamping position and a release position.

11. The cell sorting apparatus according to claim 10, wherein the clamping member is configured as an elastic member located between the first clamping drive member and the clamping body, and the elastic member is configured to clamp or release the suction release member by changing the elastic deformation of the elastic member in the radial direction by adjusting the displacement between the first clamping drive member and the clamping body.

12. The cell sorting apparatus according to claim 9, wherein the mounting assembly includes a housing member for housing the clamping means and at least one fixing member supported by the housing member, the fixing member fixing the clamping means to the housing member.

13. The cell sorting apparatus according to claim 8, wherein the selection mechanism further includes a selection illumination means connected to the mounting assembly and located at the end of the suction discharge member away from the first mounting member, and light emitted from the selection illumination means is capable of passing through the suction discharge member.

14. The cell sorting apparatus according to claim 13, wherein the suction discharge member is provided with a hollow conduit, and the selection illumination means includes a selection illumination light source installed at one end of the suction discharge member so as to extend in the axial direction of the suction discharge member, and light emitted from the selection illumination light source is capable of passing through the hollow conduit of the suction discharge member to form a projection of the suction discharge member.

15. The cell sorting apparatus according to claim 1, further comprising a cleaning assembly including a cleaning solution means for cleaning a suction discharge member and a driving means, wherein a hollow conduit is formed in the suction discharge member, the cleaning solution means includes a first cleaning solution and a second cleaning solution different from the first cleaning solution, and the driving means is connected to the suction discharge member and configured to draw the first cleaning solution into the hollow conduit to clean the suction discharge member a first predetermined number of times, and to draw the second cleaning solution into the hollow conduit to clean the suction discharge member a second predetermined number of times.

Images